AlfalfaGEDB | MsaG001906

M. sativa ssp. caerulea / MsaG001906

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005777 GO:0006810 GO:0008150 GO:0031974 GO:0031981 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043574 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001906	XP_039682599.1	89.873	237	24	0	116	352	58	294	4.99e-151	439

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001906	sp\|Q8W4B2\|APEM9_ARATH	35.159	347	173	12	25	349	16	332	1.24e-40	149

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001906	tr\|A0A072UCE5\|A0A072UCE5_MEDTR	68.129	342	88	4	20	352	19	348	3.71e-151	440

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001906	MsaG004455	0.805606	2.082955e-49	3.202643e-47
MsaG001906	MsaG007269	0.820240	1.334156e-52	2.953219e-50
MsaG001906	MsaG007279	0.818146	3.977954e-52	8.334390e-50
MsaG001906	MsaG010993	0.856081	7.941198e-62	5.335437e-59
MsaG001906	MsaG015993	0.804484	3.565972e-49	5.341804e-47
MsaG001906	MsaG016372	0.800077	2.851568e-48	3.862239e-46
MsaG001906	MsaG017567	0.852888	6.592683e-61	3.950864e-58
MsaG001906	MsaG020262	0.828375	1.666275e-54	4.605082e-52
MsaG001906	MsaG021320	0.805030	2.746279e-49	4.166565e-47
MsaG001906	MsaG023634	0.825057	1.023011e-53	2.577479e-51
MsaG001906	MsaG023982	0.802189	1.059874e-48	1.505811e-46
MsaG001906	MsaG024877	0.835906	2.332462e-56	8.027841e-54
MsaG001906	MsaG030221	0.809234	3.570109e-50	5.984823e-48
MsaG001906	MsaG033589	0.813592	4.081353e-51	7.615571e-49
MsaG001906	MsaG034866	0.828864	1.270773e-54	3.560309e-52
MsaG001906	MsaG036338	0.814064	3.216715e-51	6.073388e-49
MsaG001906	MsaG040261	0.815546	1.515094e-51	2.969523e-49
MsaG001906	MsaG045413	0.810013	2.433406e-50	4.156835e-48
MsaG001906	MsaG047185	0.800367	2.491426e-48	3.396605e-46
MsaG001906	MsaG002644	0.845597	6.907319e-59	3.227979e-56
MsaG001906	MsaG002669	0.812229	8.091365e-51	1.459222e-48
MsaG001906	MsaG002355	0.838413	5.368009e-57	1.994027e-54
MsaG001906	MsaG003294	0.804286	3.918507e-49	5.843404e-47
MsaG001906	MsaG001868	0.813572	4.122598e-51	7.688613e-49
MsaG001906	MsaG001992	0.852647	7.723197e-61	4.589236e-58
MsaG001906	MsaG011548	0.801742	1.307883e-48	1.839404e-46
MsaG001906	MsaG023430	0.825584	7.690196e-54	1.965782e-51
MsaG001906	MsaG020286	0.812878	5.847140e-51	1.071692e-48
MsaG001906	MsaG018949	0.817366	5.955668e-52	1.222966e-49
MsaG001906	MsaG031243	0.805348	2.357201e-49	3.602713e-47
MsaG001906	MsaG036115	0.829611	8.389970e-55	2.401285e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001906	MtrunA17_Chr6g0488811	68.129	342	88	4	20	352	19	348	7.14e-155	440
MsaG001906	MtrunA17_Chr3g0127571	81.818	55	10	0	1	55	192	246	3.29e-21	95.5
MsaG001906	MtrunA17_Chr7g0276111	67.273	55	18	0	1	55	198	252	5.96e-16	79.7
MsaG001906	MtrunA17_Chr5g0402551	68.627	51	16	0	1	51	188	238	6.05e-16	79.7
MsaG001906	MtrunA17_Chr8g0374701	61.818	55	21	0	1	55	182	236	8.53e-16	79.0
MsaG001906	MtrunA17_Chr4g0045261	81.395	43	8	0	1	43	191	233	3.44e-15	77.4
MsaG001906	MtrunA17_Chr7g0256001	56.364	55	24	0	1	55	195	249	3.27e-14	74.3
MsaG001906	MtrunA17_Chr5g0405421	57.895	57	22	1	1	55	150	206	3.71e-13	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001906	AT3G10572.1	35.159	347	173	12	25	349	16	332	1.26e-41	149
MsaG001906	AT3G10572.3	37.363	273	142	7	88	349	10	264	3.01e-39	141
MsaG001906	AT3G10572.2	37.363	273	142	7	88	349	10	264	3.01e-39	141
MsaG001906	AT2G41140.1	79.070	43	9	0	1	43	177	219	1.10e-15	79.3
MsaG001906	AT1G49580.1	81.395	43	8	0	1	43	204	246	2.48e-15	78.2
MsaG001906	AT5G24430.1	76.744	43	10	0	1	43	197	239	2.70e-15	78.2
MsaG001906	AT3G56760.1	76.744	43	10	0	1	43	178	220	3.52e-15	77.8
MsaG001906	AT3G50530.1	68.627	51	16	0	1	51	202	252	6.18e-15	77.0
MsaG001906	AT3G19100.1	81.395	43	8	0	1	43	198	240	6.93e-15	76.6
MsaG001906	AT3G50530.2	68.627	51	16	0	1	51	202	252	6.94e-15	76.6
MsaG001906	AT2G17890.1	61.818	55	21	0	1	55	159	213	8.59e-15	76.3
MsaG001906	AT2G46700.1	76.744	43	10	0	1	43	197	239	1.71e-14	75.5
MsaG001906	AT2G46700.2	76.744	43	10	0	1	43	16	58	2.00e-14	74.7
MsaG001906	AT2G46700.3	76.744	43	10	0	1	43	16	58	2.00e-14	74.7
MsaG001906	AT3G49370.1	74.419	43	11	0	1	43	196	238	6.51e-14	73.9
MsaG001906	AT5G66210.6	60.000	55	22	0	1	55	113	167	3.71e-13	70.9
MsaG001906	AT5G66210.4	60.000	55	22	0	1	55	113	167	3.71e-13	70.9
MsaG001906	AT5G66210.1	60.000	55	22	0	1	55	113	167	4.35e-13	71.2
MsaG001906	AT5G66210.2	60.000	55	22	0	1	55	113	167	4.35e-13	71.2
MsaG001906	AT5G66210.5	60.000	55	22	0	1	55	113	167	4.36e-13	70.9
MsaG001906	AT5G66210.3	60.000	55	22	0	1	55	113	167	4.84e-13	70.9
MsaG001906	AT4G36070.1	54.386	57	24	1	1	55	122	178	2.08e-11	65.9
MsaG001906	AT4G36070.2	54.386	57	24	1	1	55	122	178	2.40e-11	65.9

Find 87 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGAAAGACACTTTGTTCT+TGG	0.213313	1:+28459485	MsaT001906.1:CDS
TCTTGGAATCGATGAATATT+TGG	0.252724	1:+28459502	MsaT001906.1:CDS
CTTATCTGGTCATAGGAATT+TGG	0.265283	1:+28450193	MsaT001906.1:CDS
AGGTAGATCTTGCAGTTTCC+TGG	0.283266	1:+28459573	MsaT001906.1:CDS
CTTTCTTCTCAAAACATAAC+AGG	0.297497	1:-28460498	None:intergenic
TTCTTAACGGATGGAGTCTT+GGG	0.349901	1:+28459408	MsaT001906.1:CDS
AAACTCACTCTCGCTCTTCT+CGG	0.351114	1:-28456287	None:intergenic
CTGCTAAAGGATTAACTTGA+TGG	0.359510	1:-28461169	None:intergenic
TTTGGTAAATTCACGTGATA+AGG	0.363911	1:-28456611	None:intergenic
CTCACTACTGCAACACTTCA+AGG	0.370762	1:+28461203	MsaT001906.1:CDS
GTCTTTCAAATCTACTTTCA+CGG	0.373205	1:-28459472	None:intergenic
AAAATGTTGAAGGCCTTATC+TGG	0.375299	1:+28450179	None:intergenic
TAAGTTCCATTGTTATTTGT+AGG	0.378097	1:-28460284	None:intergenic
TCACGTGATAAGGGGTAGTT+TGG	0.388288	1:-28456601	None:intergenic
GTGCGAGGGTGGAGAATTAC+TGG	0.397605	1:+28450504	MsaT001906.1:CDS
AACGAATAGAGGCATGTTCC+TGG	0.402411	1:+28460387	MsaT001906.1:CDS
AATCTGAAGCAGCGATACTT+TGG	0.407859	1:+28459361	MsaT001906.1:intron
TTGGTAAATTCACGTGATAA+GGG	0.408156	1:-28456610	None:intergenic
GTTTAGGATTGTAAGAAAAC+AGG	0.420521	1:+28461097	MsaT001906.1:intron
ATCTGAAGCAGCGATACTTT+GGG	0.421804	1:+28459362	MsaT001906.1:intron
AAAGAACTACATGAGTATAA+AGG	0.422651	1:+28460314	MsaT001906.1:CDS
GAGAATGTAATAATACCATT+TGG	0.425875	1:+28456247	MsaT001906.1:CDS
TGCTGTCATTGCAGAAGAAA+AGG	0.427057	1:+28459445	MsaT001906.1:CDS
GAAGGCTTTGGTCGATTTGT+GGG	0.436305	1:+28461133	MsaT001906.1:CDS
TTTCTTAACGGATGGAGTCT+TGG	0.440466	1:+28459407	MsaT001906.1:CDS
TTTCTCGAGGAGTTTCTTAA+CGG	0.453906	1:+28459395	MsaT001906.1:CDS
AGAAGGCTTTGGTCGATTTG+TGG	0.455559	1:+28461132	MsaT001906.1:CDS
ACATAGCACAAAGCGCCTGC+TGG	0.459884	1:-28461452	None:intergenic
GATTGTGACAAAGAAGGCTT+TGG	0.462047	1:+28461121	MsaT001906.1:CDS
AAAACTGTCTGAACGAATAG+AGG	0.462342	1:+28460376	MsaT001906.1:CDS
ATATCTTCAAATGTTTCAGT+TGG	0.463395	1:+28461518	MsaT001906.1:CDS
TGTTTGCTTGTCAAGGCTCT+AGG	0.468604	1:-28460341	None:intergenic
TGAAGGCCTTATCTGGTCAT+AGG	0.469096	1:+28450186	MsaT001906.1:CDS
CGTAGGGGAGCACACCCAGC+AGG	0.476900	1:+28461437	MsaT001906.1:CDS
CAAATTCCTATGACCAGATA+AGG	0.477339	1:-28450192	None:intergenic
CTTGGTGCGTGCGGTGGCTG+TGG	0.477360	1:+28456319	MsaT001906.1:CDS
GTATATAACCGCCAAGCAAA+AGG	0.483663	1:-28461481	None:intergenic
GGTAGATCTTGCAGTTTCCT+GGG	0.497662	1:+28459574	MsaT001906.1:CDS
GAAAGCAAGCTACTTTGAAA+AGG	0.498522	1:+28460516	MsaT001906.1:CDS
AGTGAGTTTCACGATCCTCT+TGG	0.498731	1:+28456301	MsaT001906.1:CDS
TCGAGGAGTTTCTTAACGGA+TGG	0.500211	1:+28459399	MsaT001906.1:CDS
AACTGCATGTTTGCTTGTCA+AGG	0.501094	1:-28460348	None:intergenic
GCTAAGATTGTTGTACCTAG+TGG	0.505751	1:+28460443	MsaT001906.1:CDS
GAGCTTAGAGAGATAGAAAG+AGG	0.510932	1:+28456549	MsaT001906.1:CDS
TCGGCTTCAATTCCTCCAAA+TGG	0.511196	1:-28456262	None:intergenic
TGAATATTTGGAAGTTGTTG+AGG	0.515007	1:+28459514	MsaT001906.1:CDS
ACTCTCGCTCTTCTCGGTCT+CGG	0.515942	1:-28456281	None:intergenic
CGGTTATATACTGATATCAA+TGG	0.522911	1:+28461493	MsaT001906.1:CDS
TACTGGACAGAATTCTAGAT+AGG	0.523977	1:+28450521	MsaT001906.1:CDS
CATAGCACAAAGCGCCTGCT+GGG	0.527219	1:-28461451	None:intergenic
TGCCATCACACTTCTTGCAA+CGG	0.530778	1:+28459541	MsaT001906.1:CDS
CTGCTGGGTGTGCTCCCCTA+CGG	0.537032	1:-28461436	None:intergenic
CGAACTTCAAGTTAATAGCA+TGG	0.540344	1:-28460415	None:intergenic
TATTCTTGTCAGGTTGTGCG+AGG	0.541516	1:+28450489	MsaT001906.1:intron
TGCTAAAGGATTAACTTGAT+GGG	0.546105	1:-28461168	None:intergenic
CTTGTCAGGTTGTGCGAGGG+TGG	0.550541	1:+28450493	MsaT001906.1:intron
AGGGGTTGAACAGCTGCTAA+AGG	0.554743	1:-28461182	None:intergenic
GAGAGAGGGGAAAATGTTGA+AGG	0.555863	1:+28450169	None:intergenic
ATTCTTGTCAGGTTGTGCGA+GGG	0.556563	1:+28450490	MsaT001906.1:intron
TTGAAGTGTTGCAGTAGTGA+GGG	0.558023	1:-28461201	None:intergenic
CTAAGATTGTTGTACCTAGT+GGG	0.566085	1:+28460444	MsaT001906.1:CDS
CACGATCCTCTTGGTGCGTG+CGG	0.569482	1:+28456310	MsaT001906.1:CDS
AATGTAATAATACCATTTGG+AGG	0.576078	1:+28456250	MsaT001906.1:CDS
CAGCCACCGCACGCACCAAG+AGG	0.577929	1:-28456316	None:intergenic
AACCGTTGCAAGAAGTGTGA+TGG	0.581734	1:-28459543	None:intergenic
ACAGGTGATTGTGACAAAGA+AGG	0.582094	1:+28461115	MsaT001906.1:CDS
GATCCTCTTGGTGCGTGCGG+TGG	0.585585	1:+28456313	MsaT001906.1:CDS
ATTGCCTGAAGAAAATCGAC+AGG	0.589892	1:+28459610	MsaT001906.1:CDS
ATTTGTAGGCAATTGTAGAG+AGG	0.597336	1:-28460270	None:intergenic
CCAAGCATGATCTTCCCACT+AGG	0.600167	1:-28460458	None:intergenic
TGGTAAATTCACGTGATAAG+GGG	0.601130	1:-28456609	None:intergenic
AGAAGATTATGTGATTGTGA+AGG	0.603293	1:+28456572	MsaT001906.1:CDS
CAATTGCCTACAAATAACAA+TGG	0.603302	1:+28460278	MsaT001906.1:CDS
AACGGATGGAGTCTTGGGGA+TGG	0.609998	1:+28459413	MsaT001906.1:CDS
CTTGAAGTGTTGCAGTAGTG+AGG	0.617065	1:-28461202	None:intergenic
ATTCATAGGAACTTCTGAGA+AGG	0.621853	1:+28460201	MsaT001906.1:intron
GGCTGTGGTTATGACCGTCA+TGG	0.627519	1:+28456334	MsaT001906.1:CDS
TTATGACCGTCATGGTGTAA+CGG	0.632533	1:+28456342	MsaT001906.1:CDS
GAATAACGTCTACATTGTGA+TGG	0.632991	1:+28450244	MsaT001906.1:CDS
CCTAGTGGGAAGATCATGCT+TGG	0.634227	1:+28460458	MsaT001906.1:CDS
GTCATTGCAGAAGAAAAGGA+AGG	0.637291	1:+28459449	MsaT001906.1:CDS
TCTTAACGGATGGAGTCTTG+GGG	0.642495	1:+28459409	MsaT001906.1:CDS
GGGTGTTCGAGAATTTCTCG+AGG	0.663732	1:+28459382	MsaT001906.1:CDS
GTTAATAGCATGGAAACACC+AGG	0.672445	1:-28460405	None:intergenic
AGAGGCAACTTGAGACGATG+TGG	0.683503	1:-28460252	None:intergenic
TGAAGTGTTGCAGTAGTGAG+GGG	0.688199	1:-28461200	None:intergenic
TGTCCACCGTTACACCATGA+CGG	0.690909	1:-28456348	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	28450182	28461553	28450182	ID=MsaG001906
Chr1	mRNA	28450182	28461553	28450182	ID=MsaT001906.1;Parent=MsaG001906
Chr1	exon	28450182	28450267	28450182	ID=MsaT001906.1.exon1;Parent=MsaT001906.1
Chr1	CDS	28450182	28450267	28450182	ID=cds.MsaT001906.1;Parent=MsaT001906.1
Chr1	exon	28450501	28450542	28450501	ID=MsaT001906.1.exon2;Parent=MsaT001906.1
Chr1	CDS	28450501	28450542	28450501	ID=cds.MsaT001906.1;Parent=MsaT001906.1
Chr1	exon	28456225	28456363	28456225	ID=MsaT001906.1.exon3;Parent=MsaT001906.1
Chr1	CDS	28456225	28456363	28456225	ID=cds.MsaT001906.1;Parent=MsaT001906.1
Chr1	exon	28456546	28456619	28456546	ID=MsaT001906.1.exon4;Parent=MsaT001906.1
Chr1	CDS	28456546	28456619	28456546	ID=cds.MsaT001906.1;Parent=MsaT001906.1
Chr1	exon	28459370	28459631	28459370	ID=MsaT001906.1.exon5;Parent=MsaT001906.1
Chr1	CDS	28459370	28459631	28459370	ID=cds.MsaT001906.1;Parent=MsaT001906.1
Chr1	exon	28460209	28460537	28460209	ID=MsaT001906.1.exon6;Parent=MsaT001906.1
Chr1	CDS	28460209	28460537	28460209	ID=cds.MsaT001906.1;Parent=MsaT001906.1
Chr1	exon	28461103	28461224	28461103	ID=MsaT001906.1.exon7;Parent=MsaT001906.1
Chr1	CDS	28461103	28461224	28461103	ID=cds.MsaT001906.1;Parent=MsaT001906.1
Chr1	exon	28461432	28461553	28461432	ID=MsaT001906.1.exon8;Parent=MsaT001906.1
Chr1	CDS	28461432	28461553	28461432	ID=cds.MsaT001906.1;Parent=MsaT001906.1

Gene Sequence

>MsaT001906.1

ATGTTGAAGGCCTTATCTGGTCATAGGAATTTGGTCAAGTTTTATGATGCATTTGAGGATGTGAATAACGTCTACATTGTGATGGAGTTGTGCGAGGGTGGAGAATTACTGGACAGAATTCTAGATAGAATCAACAACATAGAGAAAGAAGAGAATGTAATAATACCATTTGGAGGAATTGAAGCCGAGACCGAGAAGAGCGAGAGTGAGTTTCACGATCCTCTTGGTGCGTGCGGTGGCTGTGGTTATGACCGTCATGGTGTAACGAATGAGCTTAGAGAGATAGAAAGAGGAGAAGATTATGTGATTGTGAAGGAAACTACCAAACTACCCCTTATCACCAGCGATACTTTGGGTGTTCGAGAATTTCTCGAGGAGTTTCTTAACGGATGGAGTCTTGGGGATGGACAATATCATGCTGTCATTGCAGAAGAAAAGGAAGGCCGTGAAAGTAGATTTGAAAGACACTTTGTTCTTGGAATCGATGAATATTTGGAAGTTGTTGAGGTCTATGCCATCACACTTCTTGCAACGGTTTTAAAAGAGGTAGATCTTGCAGTTTCCTGGGTTGAAAATGCTCCATTGCCTGAAGAAAATCGACAGGAACTTCTGAGAAGGTTACGCTCTATACATTCTCTTAGAAGTACCACATCGTCTCAAGTTGCCTCTCTACAATTGCCTACAAATAACAATGGAACTTATTCTTCGAAAGAACTACATGAGTATAAAGGATCACCTAGAGCCTTGACAAGCAAACATGCAGTTGTCTCAAAACTGTCTGAACGAATAGAGGCATGTTCCTGGTGTTTCCATGCTATTAACTTGAAGTTCGCTAATGCTAAGATTGTTGTACCTAGTGGGAAGATCATGCTTGGTTGTTTGATCTTGTTCGCCTGTTATGTTTTGAGAAGAAAGCAAGCTACTTTGAAAAGGATTGTAAGAAAACAGGTGATTGTGACAAAGAAGGCTTTGGTCGATTTGTGGGAGCTTGCATTTTCCCATCAAGTTAATCCTTTAGCAGCTGTTCAACCCCTCACTACTGCAACACTTCAAGGCAGCCGTAGGGGAGCACACCCAGCAGGCGCTTTGTGCTATGTTGTAAACCTTTTGCTTGGCGGTTATATACTGATATCAATGGCGATATCTTCAAATGTTTCAGTTGGATTTATTGTGTGA

Protein sequence

>MsaT001906.1

MLKALSGHRNLVKFYDAFEDVNNVYIVMELCEGGELLDRILDRINNIEKEENVIIPFGGIEAETEKSESEFHDPLGACGGCGYDRHGVTNELREIERGEDYVIVKETTKLPLITSDTLGVREFLEEFLNGWSLGDGQYHAVIAEEKEGRESRFERHFVLGIDEYLEVVEVYAITLLATVLKEVDLAVSWVENAPLPEENRQELLRRLRSIHSLRSTTSSQVASLQLPTNNNGTYSSKELHEYKGSPRALTSKHAVVSKLSERIEACSWCFHAINLKFANAKIVVPSGKIMLGCLILFACYVLRRKQATLKRIVRKQVIVTKKALVDLWELAFSHQVNPLAAVQPLTTATLQGSRRGAHPAGALCYVVNLLLGGYILISMAISSNVSVGFIV*