AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG001912

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001912 MtrunA17_Chr1g0169161 75.482 363 37 2 1 337 1 337 4.90e-171 478
MsaG001912 MtrunA17_Chr3g0125161 74.167 120 31 0 2 121 13 132 1.16e-57 184
MsaG001912 MtrunA17_Chr1g0169081 80.952 105 20 0 5 109 2 106 3.06e-54 180
MsaG001912 MtrunA17_Chr3g0125151 55.200 125 28 2 234 332 1 123 7.26e-42 142
MsaG001912 MtrunA17_Chr1g0169171 75.294 85 21 0 36 120 3 87 2.28e-37 135
MsaG001912 MtrunA17_Chr1g0154251 26.351 148 105 3 12 158 27 171 1.74e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG001912 AT3G06160.5 24.012 329 218 6 9 332 8 309 1.29e-17 82.8
MsaG001912 AT3G06160.4 24.012 329 218 6 9 332 8 309 1.29e-17 82.8
MsaG001912 AT3G06160.3 24.012 329 218 6 9 332 8 309 1.29e-17 82.8
MsaG001912 AT3G06160.1 24.012 329 218 6 9 332 23 324 1.66e-17 82.4
MsaG001912 AT3G06160.2 24.012 329 218 6 9 332 67 368 3.54e-17 82.0
MsaG001912 AT5G60140.1 23.653 334 220 7 11 331 15 326 1.10e-11 65.5
MsaG001912 AT3G18960.2 28.205 156 107 4 11 164 30 182 5.36e-11 61.6
MsaG001912 AT3G18960.1 35.294 102 63 2 11 111 30 129 6.24e-11 61.6

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCAGAGTGCTTATCTTTA+AGG 0.100384 1:-28555296 None:intergenic
TCAAAACTTACATGCTTTGC+AGG 0.294940 1:-28556192 None:intergenic
ACCAAGTTCACAAAAGCATT+TGG 0.331753 1:-28555642 None:intergenic
AAAGGATTATACCAATTCAT+TGG 0.334731 1:-28556436 None:intergenic
TGAGGAGGAAAATAATAATA+TGG 0.339726 1:+28555905 MsaT001912.1:CDS
TAGAGAAGAAAATTGTTTAG+TGG 0.355645 1:+28555785 MsaT001912.1:CDS
CATCAATTTCATCATCTTCA+AGG 0.360750 1:-28556353 None:intergenic
TGAACTTGGTGCGTTCAAAA+AGG 0.361173 1:+28555655 MsaT001912.1:CDS
TGATAAGGGTGCACATTGTT+AGG 0.364925 1:+28557019 MsaT001912.1:CDS
GATGGTGATGATGTTGATTA+TGG 0.366118 1:+28555948 MsaT001912.1:CDS
CTCTTCCTCTACGATCTCTT+AGG 0.380260 1:-28555698 None:intergenic
GCAAAGAAATGTGGATTTCC+TGG 0.382967 1:-28556393 None:intergenic
GGAAGATGAGGAAGAGGAAG+AGG 0.385448 1:+28555926 MsaT001912.1:CDS
CCAAGTTCACAAAAGCATTT+GGG 0.414978 1:-28555641 None:intergenic
GAGAAGCAATAGAAATGATA+AGG 0.417868 1:+28557004 MsaT001912.1:CDS
TCTTTACAGACCCAATGAAT+TGG 0.436856 1:+28556425 MsaT001912.1:CDS
AAGGATTATACCAATTCATT+GGG 0.448200 1:-28556435 None:intergenic
CATGTCAAAGCGCGTCGTAT+TGG 0.452247 1:+28555726 MsaT001912.1:CDS
TAATATGGAAGATGAGGAAG+AGG 0.461266 1:+28555920 MsaT001912.1:CDS
GACATCGGAAGCAGCTCTGC+AGG 0.476732 1:+28556324 MsaT001912.1:CDS
GAGATCGTAGAGGAAGAGAT+TGG 0.486399 1:+28555703 MsaT001912.1:CDS
ATCAATGTGTGAAAAGATGC+CGG 0.497263 1:+28555875 MsaT001912.1:CDS
TCAATGTGTGAAAAGATGCC+GGG 0.501346 1:+28555876 MsaT001912.1:CDS
TACAAGGAAAATAATAAAAG+TGG 0.503753 1:+28556152 MsaT001912.1:CDS
AATGGCCGAGTCTTGGTGCT+AGG 0.508826 1:+28556907 MsaT001912.1:CDS
AGATAAGCACTCTGAGAGAA+TGG 0.511759 1:+28555302 MsaT001912.1:CDS
GATGAGGAAGAGGAAGAGGA+TGG 0.519418 1:+28555930 MsaT001912.1:CDS
AAAGATGCCGGGGATGGATG+AGG 0.521214 1:+28555887 MsaT001912.1:CDS
AGAGAGGTCAAATACGTACT+TGG 0.526584 1:+28556881 MsaT001912.1:CDS
AGAAGCAATAGAAATGATAA+GGG 0.545719 1:+28557005 MsaT001912.1:CDS
ATGGCCGAGTCTTGGTGCTA+GGG 0.545802 1:+28556908 MsaT001912.1:CDS
AAGGGTGCACATTGTTAGGA+AGG 0.551559 1:+28557023 MsaT001912.1:CDS
AACAATGCAAAGGAGTATCC+TGG 0.558136 1:+28555258 MsaT001912.1:CDS
AAATAATAATATGGAAGATG+AGG 0.565051 1:+28555914 MsaT001912.1:CDS
ACAGAGTGAAAATGTGTACA+AGG 0.566220 1:+28556136 MsaT001912.1:CDS
GTGTGAAAAGATGCCGGGGA+TGG 0.569701 1:+28555881 MsaT001912.1:CDS
GACGATGAGGGTGATGATGA+GGG 0.579768 1:+28556014 MsaT001912.1:CDS
TGACGATGAGGGTGATGATG+AGG 0.595776 1:+28556013 MsaT001912.1:CDS
GCCACCCTAGCACCAAGACT+CGG 0.602379 1:-28556912 None:intergenic
GCCGAGTCTTGGTGCTAGGG+TGG 0.603546 1:+28556911 MsaT001912.1:CDS
GTATGTCTTGTGCTGATCTG+TGG 0.608903 1:-28556853 None:intergenic
TGATGATGATGATGCTGAAG+AGG 0.610168 1:+28555980 MsaT001912.1:CDS
GATGATGATGATGCTGAAGA+GGG 0.617100 1:+28555981 MsaT001912.1:CDS
GGTGATGATAGTGACGATGA+GGG 0.623737 1:+28556002 MsaT001912.1:CDS
TGATGATGATGTGATGGCAG+AGG 0.626963 1:+28556046 MsaT001912.1:CDS
AACTATGACTAACAATGCAA+AGG 0.632234 1:+28555248 None:intergenic
GATGCCGGGGATGGATGAGG+AGG 0.641755 1:+28555890 MsaT001912.1:CDS
GGGTGATGATAGTGACGATG+AGG 0.647049 1:+28556001 MsaT001912.1:CDS
GCTGAAATGTACATTCAACC+AGG 0.656893 1:+28556375 MsaT001912.1:CDS
TGTGAACTTGTAATGCGTAA+CGG 0.682308 1:+28556982 MsaT001912.1:CDS
ACAAGACATACCGAAAAGAG+AGG 0.689496 1:+28556865 MsaT001912.1:CDS
CAATGTGTGAAAAGATGCCG+GGG 0.703528 1:+28555877 MsaT001912.1:CDS
TTTATCCTAAGAGATCGTAG+AGG 0.735718 1:+28555693 MsaT001912.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 28555252 28557059 28555252 ID=MsaG001912
Chr1 mRNA 28555252 28557059 28555252 ID=MsaT001912.1;Parent=MsaG001912
Chr1 exon 28555252 28555323 28555252 ID=MsaT001912.1.exon1;Parent=MsaT001912.1
Chr1 CDS 28555252 28555323 28555252 ID=cds.MsaT001912.1;Parent=MsaT001912.1
Chr1 exon 28555636 28556203 28555636 ID=MsaT001912.1.exon2;Parent=MsaT001912.1
Chr1 CDS 28555636 28556203 28555636 ID=cds.MsaT001912.1;Parent=MsaT001912.1
Chr1 exon 28556322 28556446 28556322 ID=MsaT001912.1.exon3;Parent=MsaT001912.1
Chr1 CDS 28556322 28556446 28556322 ID=cds.MsaT001912.1;Parent=MsaT001912.1
Chr1 exon 28556811 28557059 28556811 ID=MsaT001912.1.exon4;Parent=MsaT001912.1
Chr1 CDS 28556811 28557059 28556811 ID=cds.MsaT001912.1;Parent=MsaT001912.1
Gene Sequence

>MsaT001912.1

ATGACTAACAATGCAAAGGAGTATCCTGGCTTTTTCAAAGTCTTCCTTAAAGATAAGCACTCTGAGAGAATGCTTATCCCAAATGCTTTTGTGAACTTGGTGCGTTCAAAAAGGAGGGTAATAAAGGATTTTATCCTAAGAGATCGTAGAGGAAGAGATTGGCATGTCAAAGCGCGTCGTATTGGTGACGAGTTTTATTTTGATGATGGTTGGAAGAAATTTAGAGAAGAAAATTGTTTAGTGGAGAATGACTTCCTTGTTTTTACTCATATTGAAAACAATATATTCAAGTTCAAAATTTTTGAACTATCATCAATGTGTGAAAAGATGCCGGGGATGGATGAGGAGGAAAATAATAATATGGAAGATGAGGAAGAGGAAGAGGATGGTGATGATGTTGATTATGGTGACAATGATGATGATGATGATGCTGAAGAGGGTGATGATAGTGACGATGAGGGTGATGATGAGGGTAATGATGATGATGATGATGTGATGGCAGAGGAAGAAAAAGATGATAACGATGATGAGATTAGTGACGATGATGTCTCTGAGAATATAATGAAAGAAAAACAGAGTGAAAATGTGTACAAGGAAAATAATAAAAGTGGACATCAACATTGTAGAACCTGCAAAGCATGGGACATCGGAAGCAGCTCTGCAGGTTCAAACCTTGAAGATGATGAAATTGATGCTGAAATGTACATTCAACCAGGAAATCCACATTTCTTTGCAAAACATAGTCTTTACAGACCCAATGAATTGGATATTAAAAGCAGTGACATAGCAGCGTATCATCACATCTTGCCACAGATCAGCACAAGACATACCGAAAAGAGAGGTCAAATACGTACTTGGAAAAATGGCCGAGTCTTGGTGCTAGGGTGGCAAGACTTTTGTAGAAAGTGCAAGATAACAGAAAATGATAGATGCTTGTGTGAACTTGTAATGCGTAACGGAGAAGCAATAGAAATGATAAGGGTGCACATTGTTAGGAAGGTTAACAAGAAATGA
Protein sequence

>MsaT001912.1

MTNNAKEYPGFFKVFLKDKHSERMLIPNAFVNLVRSKRRVIKDFILRDRRGRDWHVKARRIGDEFYFDDGWKKFREENCLVENDFLVFTHIENNIFKFKIFELSSMCEKMPGMDEEENNNMEDEEEEEDGDDVDYGDNDDDDDAEEGDDSDDEGDDEGNDDDDDVMAEEEKDDNDDEISDDDVSENIMKEKQSENVYKENNKSGHQHCRTCKAWDIGSSSAGSNLEDDEIDAEMYIQPGNPHFFAKHSLYRPNELDIKSSDIAAYHHILPQISTRHTEKRGQIRTWKNGRVLVLGWQDFCRKCKITENDRCLCELVMRNGEAIEMIRVHIVRKVNKK*