AlfalfaGEDB | MsaG001913

M. sativa ssp. caerulea / MsaG001913

KEGG: K21554
GO: -
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001913	XP_039686178.1	72.903	155	42	0	308	462	179	333	1.01e-74	258
MsaG001913	XP_039686178.1	79.793	386	41	3	78	463	328	676	0.0	562

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001913	sp\|Q9FHH1\|Y5772_ARATH	21.693	378	209	13	91	461	2	299	9.67e-11	66.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001913	tr\|A0A072VGK8\|A0A072VGK8_MEDTR	81.720	372	31	3	92	463	1	335	0.0	553

TFs/TRs:

Gene ID	Type	Classification
MsaG001913	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001233	MsaG001913	0.805873	1.830922e-49	2.832891e-47
MsaG001913	MsaG021558	0.800997	1.855931e-48	2.566397e-46
MsaG001913	MsaG028831	0.813204	4.962959e-51	9.170742e-49
MsaG001913	MsaG032509	0.804741	3.153601e-49	4.752393e-47
MsaG001913	MsaG025090	0.801184	1.700183e-48	2.360989e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001913	MtrunA17_Chr1g0169171	79.056	339	34	3	125	463	1	302	1.29e-172	486
MsaG001913	MtrunA17_Chr1g0169081	57.568	370	106	5	96	462	2	323	8.99e-128	373
MsaG001913	MtrunA17_Chr1g0169161	72.903	155	42	0	308	462	179	333	2.98e-82	257
MsaG001913	MtrunA17_Chr3g0125151	80.000	125	23	1	337	461	1	123	1.09e-68	214
MsaG001913	MtrunA17_Chr3g0125161	77.941	136	30	0	89	224	9	144	1.40e-64	205

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG001913	AT5G57720.1	21.693	378	209	13	91	461	2	299	9.83e-12	66.2

Find 85 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGAAAATTCAACATAAAA+TGG	0.171412	1:-28565444	None:intergenic
TATAAATAACATACATGTTT+TGG	0.264573	1:-28567102	None:intergenic
TTTATTTACTTGCCCGGTTT+TGG	0.272955	1:-28565291	None:intergenic
ATTTCATCATCTTCAAGATT+TGG	0.287026	1:-28567261	None:intergenic
AGGAAGACTTTGAAGAAGTT+AGG	0.307444	1:-28566273	None:intergenic
TGCTAAGGGTGCACGTTGTT+AGG	0.311521	1:+28567964	MsaT001913.1:CDS
ACCTTTGGAATATTGAGTTT+TGG	0.323910	1:-28565528	None:intergenic
AGAGAGGAGAAATACGTAAA+TGG	0.332365	1:+28567826	MsaT001913.1:CDS
GCATAGAAGTATGGATTTCC+AGG	0.335888	1:-28567306	None:intergenic
CACTTCCTCTATGATCTCTT+AGG	0.351749	1:-28566569	None:intergenic
AATGTTTGTTGGAAGGTTCT+AGG	0.355424	1:-28565716	None:intergenic
TGACAACAGACCAAACATAT+TGG	0.391564	1:+28567338	MsaT001913.1:CDS
AGGAAGAACAAAATGTTTGT+TGG	0.400016	1:-28565727	None:intergenic
TGTGAATAGCATTATAGCTC+TGG	0.401494	1:+28565906	MsaT001913.1:CDS
TCAGAATGAGTTTCCAAAAC+CGG	0.415772	1:+28565278	None:intergenic
GCTAAGGGTGCACGTTGTTA+GGG	0.434379	1:+28567965	MsaT001913.1:CDS
TTGCGGTAATACATGATGAT+AGG	0.439512	1:-28567781	None:intergenic
TACTAATACGATGGAGGATG+AGG	0.463591	1:+28566788	MsaT001913.1:CDS
TACGATGGAGGATGAGGAAG+AGG	0.464381	1:+28566794	MsaT001913.1:CDS
GCAAATCAATTGAAATGCTA+AGG	0.464591	1:+28567949	MsaT001913.1:CDS
CCTTGCAGTGGCAACATACA+AGG	0.465539	1:-28567609	None:intergenic
TTCAAAACAAAGGCTATTGA+AGG	0.469441	1:+28566539	MsaT001913.1:CDS
AATAAAGTTTGTTGCCGATA+GGG	0.473847	1:-28566611	None:intergenic
GGAAGACTTTGAAGAAGTTA+GGG	0.478785	1:-28566272	None:intergenic
GTGAATAGCATTATAGCTCT+GGG	0.481377	1:+28565907	MsaT001913.1:CDS
CATGTCAAAGTGCGCCCTAT+CGG	0.483624	1:+28566597	MsaT001913.1:CDS
TGGCATTGTTAGTCATAACT+TGG	0.483952	1:-28566242	None:intergenic
CACACATTGAGGATAGTTCA+AGG	0.489279	1:-28566734	None:intergenic
AGAACAAAATGTTTGTTGGA+AGG	0.492978	1:-28565723	None:intergenic
GCATTATAGCTCTGGGGCCA+AGG	0.498210	1:+28565914	MsaT001913.1:CDS
GATATTGGTTTAGGGAGCAT+TGG	0.500691	1:+28567222	MsaT001913.1:intron
GAGGATGAGGAAGAGGAAGA+TGG	0.501826	1:+28566801	MsaT001913.1:CDS
TGATGTATTGAAGTGAGAAA+AGG	0.502412	1:-28565747	None:intergenic
GAGATCATAGAGGAAGTGAT+TGG	0.510228	1:+28566574	MsaT001913.1:CDS
AAAGAAGCACTCTGATAGAA+TGG	0.512843	1:+28566299	MsaT001913.1:CDS
AGGAATAAAGGAGATGGAAA+GGG	0.516562	1:+28567041	MsaT001913.1:CDS
GAAATACGTAAATGGAAAGA+TGG	0.523151	1:+28567834	MsaT001913.1:CDS
AAGGAATAAAGGAGATGGAA+AGG	0.528969	1:+28567040	MsaT001913.1:CDS
CGATGACGTTGATGAGGAGA+AGG	0.531028	1:+28566893	MsaT001913.1:CDS
CAGAATGAGTTTCCAAAACC+GGG	0.533035	1:+28565279	None:intergenic
GAAGAAGTTAGGGAACTCCT+TGG	0.540851	1:-28566262	None:intergenic
GTCGAGTATTTGTGCGAGGG+TGG	0.540995	1:+28567856	MsaT001913.1:CDS
ATCAGCTTCACAAAAGCCGT+TGG	0.545718	1:-28566513	None:intergenic
CGATGATGTTGATGAGGAGG+AGG	0.548787	1:+28566983	MsaT001913.1:CDS
GCTGAAATGTATATTCAGCC+TGG	0.549138	1:+28567288	MsaT001913.1:CDS
TCAGCTTCACAAAAGCCGTT+GGG	0.551363	1:-28566512	None:intergenic
TATCAGAGTGCTTCTTTGTG+AGG	0.557625	1:-28566293	None:intergenic
AAATAAAGTTTGTTGCCGAT+AGG	0.559250	1:-28566612	None:intergenic
AAAGATGAAGGTGATGGACG+AGG	0.565087	1:+28566758	MsaT001913.1:CDS
CTCAATGTGTGAAAAGATGA+AGG	0.571790	1:+28566746	MsaT001913.1:CDS
TATTTCGAAGAATGTCATCA+AGG	0.573351	1:+28567555	MsaT001913.1:CDS
TGTGAACTTGTGTTGCGTAA+AGG	0.574931	1:+28567927	MsaT001913.1:CDS
CCTTGTATGTTGCCACTGCA+AGG	0.576526	1:+28567609	MsaT001913.1:CDS
GGAGGAAAATACTAATACGA+TGG	0.576880	1:+28566779	MsaT001913.1:CDS
CGTTGATGAGGAGAAGGATG+AGG	0.589997	1:+28566899	MsaT001913.1:CDS
ATGGTAGATGAAGAAGAAGA+TGG	0.592976	1:+28566951	MsaT001913.1:CDS
CAAATCAATTGAAATGCTAA+GGG	0.593705	1:+28567950	MsaT001913.1:CDS
TGATGATTACACAATGGTAG+AGG	0.597750	1:+28566851	MsaT001913.1:CDS
TCGCAGGATGTTCAAAGTAA+CGG	0.598432	1:+28567750	MsaT001913.1:intron
ACAAAACATATTGAAAAGAG+AGG	0.601836	1:+28567810	MsaT001913.1:CDS
TGATATGCTTTGTAAAGCAA+AGG	0.607441	1:-28567585	None:intergenic
TCGAGTATTTGTGCGAGGGT+GGG	0.609634	1:+28567857	MsaT001913.1:CDS
AGATGCTGAGGGTTCTGTGT+TGG	0.612042	1:-28565490	None:intergenic
TTTCAATGTGTGTGAAAACA+AGG	0.612200	1:-28566689	None:intergenic
TAAGGAAGATGCAGATGCTG+AGG	0.615464	1:-28565502	None:intergenic
ACCAAAACTCAATATTCCAA+AGG	0.616253	1:+28565527	MsaT001913.1:CDS
GGAAAATACTAATACGATGG+AGG	0.618407	1:+28566782	MsaT001913.1:CDS
ATAACAATTATACCTTGCAG+TGG	0.625581	1:-28567621	None:intergenic
AAGGAAGATGCAGATGCTGA+GGG	0.629715	1:-28565501	None:intergenic
AGCTGATGCATTCAAAACAA+AGG	0.631482	1:+28566529	MsaT001913.1:CDS
TAACGATGATGTTGATGAGG+AGG	0.631678	1:+28566980	MsaT001913.1:CDS
TTTATCCTAAGAGATCATAG+AGG	0.632452	1:+28566564	MsaT001913.1:CDS
TCTACTGCAGCTTATCCCAA+CGG	0.637580	1:+28566497	MsaT001913.1:intron
TGATAACGATGATGTTGATG+AGG	0.638893	1:+28566977	MsaT001913.1:CDS
GATGAAGGTGATGGACGAGG+AGG	0.642889	1:+28566761	MsaT001913.1:CDS
AGTTATGACTAACAATGCCA+AGG	0.645282	1:+28566245	MsaT001913.1:CDS
GTGTGAAAAGATGAAGGTGA+TGG	0.648653	1:+28566752	MsaT001913.1:CDS
GATGGTCGAGTATTTGTGCG+AGG	0.649148	1:+28567852	MsaT001913.1:CDS
ATGGTCGAGTATTTGTGCGA+GGG	0.655823	1:+28567853	MsaT001913.1:CDS
CTTGCAGTGGCAACATACAA+GGG	0.670891	1:-28567608	None:intergenic
ACAATGGTAGAGGAAGAAGA+TGG	0.676224	1:+28566861	MsaT001913.1:CDS
TGATAACGATGACGTTGATG+AGG	0.684754	1:+28566887	MsaT001913.1:CDS
TGAATAGCATTATAGCTCTG+GGG	0.713754	1:+28565908	MsaT001913.1:CDS
TGATGATGATGATTACACAA+TGG	0.769323	1:+28566845	MsaT001913.1:CDS
TGATGATGATGATTACACAA+TGG	0.769323	1:+28566932	MsaT001913.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	28565283	28567995	28565283	ID=MsaG001913
Chr1	mRNA	28565283	28567995	28565283	ID=MsaT001913.1;Parent=MsaG001913
Chr1	exon	28565283	28565304	28565283	ID=MsaT001913.1.exon1;Parent=MsaT001913.1
Chr1	CDS	28565283	28565304	28565283	ID=cds.MsaT001913.1;Parent=MsaT001913.1
Chr1	exon	28565427	28565548	28565427	ID=MsaT001913.1.exon2;Parent=MsaT001913.1
Chr1	CDS	28565427	28565548	28565427	ID=cds.MsaT001913.1;Parent=MsaT001913.1
Chr1	exon	28565713	28565777	28565713	ID=MsaT001913.1.exon3;Parent=MsaT001913.1
Chr1	CDS	28565713	28565777	28565713	ID=cds.MsaT001913.1;Parent=MsaT001913.1
Chr1	exon	28565885	28565937	28565885	ID=MsaT001913.1.exon4;Parent=MsaT001913.1
Chr1	CDS	28565885	28565937	28565885	ID=cds.MsaT001913.1;Parent=MsaT001913.1
Chr1	exon	28566238	28566320	28566238	ID=MsaT001913.1.exon5;Parent=MsaT001913.1
Chr1	CDS	28566238	28566320	28566238	ID=cds.MsaT001913.1;Parent=MsaT001913.1
Chr1	exon	28566507	28567110	28566507	ID=MsaT001913.1.exon6;Parent=MsaT001913.1
Chr1	CDS	28566507	28567110	28566507	ID=cds.MsaT001913.1;Parent=MsaT001913.1
Chr1	exon	28567235	28567359	28567235	ID=MsaT001913.1.exon7;Parent=MsaT001913.1
Chr1	CDS	28567235	28567359	28567235	ID=cds.MsaT001913.1;Parent=MsaT001913.1
Chr1	exon	28567553	28567630	28567553	ID=MsaT001913.1.exon8;Parent=MsaT001913.1
Chr1	CDS	28567553	28567630	28567553	ID=cds.MsaT001913.1;Parent=MsaT001913.1
Chr1	exon	28567756	28567995	28567756	ID=MsaT001913.1.exon9;Parent=MsaT001913.1
Chr1	CDS	28567756	28567995	28567756	ID=cds.MsaT001913.1;Parent=MsaT001913.1

Gene Sequence

>MsaT001913.1

ATGAGTTTCCAAAACCGGGCAAACATAGCATCATCAACTCCATTTTATGTTGAATTTTCAATCAAAGCTCATCAATATTTCTACTCCAACACAGAACCCTCAGCATCTGCATCTTCCTTAACACCAAAACTCAATATTCCAAAGGAACCTAGAACCTTCCAACAAACATTTTGTTCTTCCTTTTCTCACTTCAATACATCAGATAAAATGTACAAAATATTTGTTAGCTTTGTGAATAGCATTATAGCTCTGGGGCCAAGGTATAACCAAGTTATGACTAACAATGCCAAGGAGTTCCCTAACTTCTTCAAAGTCTTCCTCACAAAGAAGCACTCTGATAGAATGCTTATCCCAACGGCTTTTGTGAAGCTGATGCATTCAAAACAAAGGCTATTGAAGGATTTTATCCTAAGAGATCATAGAGGAAGTGATTGGCATGTCAAAGTGCGCCCTATCGGCAACAAACTTTATTTTGATGATGGTTGGAAACTATTTAGAGAAGAAAATTCTTTAGAGAACAATGACTTCCTTGTTTTCACACACATTGAAAACAATGTATTCAAGTTCAAAATCCTTGAACTATCCTCAATGTGTGAAAAGATGAAGGTGATGGACGAGGAGGAAAATACTAATACGATGGAGGATGAGGAAGAGGAAGATGGTGATGATGATTATGATAATCATGATGATGATGATTACACAATGGTAGAGGAAGAAGATGGTGATGATAACGATGACGTTGATGAGGAGAAGGATGAGGATGATGTTCATGATGATGATGATTACACAATGGTAGATGAAGAAGAAGATGGTGATGATAACGATGATGTTGATGAGGAGGAGGACGAGAATGTTGCTGATATAATAATGAAAGAAAAAAGGAATAAAGGAGATGGAAAGGGAATCAGCAGAAGTTGTGAACATCAACATTGTAGAAGCTCCAAAACATGGAGCATTGGAAGTAGCTCTGCAGCTCCAAATCTTGAAGATGATGAAATTGATGCTGAAATGTATATTCAGCCTGGAAATCCATACTTCTATGCAAAACAATATGACAACAGACCAAACATATTGAATATTTCGAAGAATGTCATCAAGGATTTCTGCCTTTGCTTTACAAAGCATATCACCCTTGTATGTTGCCACTGCAAGGATGTTCAAAGTAACGGAATAGCAGCCTATCATCATGTATTACCGCAAATGAGCACAAAACATATTGAAAAGAGAGGAGAAATACGTAAATGGAAAGATGGTCGAGTATTTGTGCGAGGGTGGGAAGATTTCTGCAGAAAGAGCAAGATCGCAGAAAATGATAGATGCTTGTGTGAACTTGTGTTGCGTAAAGGCAAATCAATTGAAATGCTAAGGGTGCACGTTGTTAGGGAAAAATGA

Protein sequence

>MsaT001913.1

MSFQNRANIASSTPFYVEFSIKAHQYFYSNTEPSASASSLTPKLNIPKEPRTFQQTFCSSFSHFNTSDKMYKIFVSFVNSIIALGPRYNQVMTNNAKEFPNFFKVFLTKKHSDRMLIPTAFVKLMHSKQRLLKDFILRDHRGSDWHVKVRPIGNKLYFDDGWKLFREENSLENNDFLVFTHIENNVFKFKILELSSMCEKMKVMDEEENTNTMEDEEEEDGDDDYDNHDDDDYTMVEEEDGDDNDDVDEEKDEDDVHDDDDYTMVDEEEDGDDNDDVDEEEDENVADIIMKEKRNKGDGKGISRSCEHQHCRSSKTWSIGSSSAAPNLEDDEIDAEMYIQPGNPYFYAKQYDNRPNILNISKNVIKDFCLCFTKHITLVCCHCKDVQSNGIAAYHHVLPQMSTKHIEKRGEIRKWKDGRVFVRGWEDFCRKSKIAENDRCLCELVLRKGKSIEMLRVHVVREK*