AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002071

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002071 MtrunA17_Chr8g0389241 54.321 81 35 2 37 116 106 185 4.04e-21 85.1
MsaG002071 MtrunA17_Chr5g0440731 56.944 72 28 1 38 106 107 178 6.89e-20 82.0
MsaG002071 MtrunA17_Chr5g0440741 56.944 72 28 1 38 106 102 173 1.77e-19 80.9
MsaG002071 MtrunA17_Chr2g0331031 54.688 64 29 0 37 100 110 173 4.20e-19 80.5
MsaG002071 MtrunA17_Chr6g0488471 48.315 89 36 3 37 116 98 185 8.03e-19 79.3
MsaG002071 MtrunA17_Chr3g0135671 71.739 46 13 0 37 82 92 137 2.03e-18 77.4
MsaG002071 MtrunA17_Chr7g0241761 46.835 79 42 0 37 115 141 219 2.10e-18 78.6
MsaG002071 MtrunA17_Chr3g0104071 40.708 113 53 2 2 100 99 211 5.57e-18 77.4
MsaG002071 MtrunA17_Chr5g0429521 54.688 64 29 0 37 100 133 196 6.91e-18 77.0
MsaG002071 MtrunA17_Chr5g0409841 51.429 70 32 1 38 105 94 163 9.17e-18 75.5
MsaG002071 MtrunA17_Chr4g0055931 47.059 68 36 0 37 104 96 163 9.18e-18 76.6
MsaG002071 MtrunA17_Chr1g0210471 51.562 64 31 0 37 100 125 188 3.78e-17 73.9
MsaG002071 MtrunA17_Chr3g0092621 41.304 92 53 1 10 100 57 148 4.07e-17 72.8
MsaG002071 MtrunA17_Chr4g0062741 50.000 64 32 0 37 100 139 202 4.24e-17 74.7
MsaG002071 MtrunA17_Chr5g0415191 53.731 67 31 0 37 103 103 169 6.91e-17 74.3
MsaG002071 MtrunA17_Chr1g0162081 52.381 63 30 0 38 100 124 186 7.87e-17 73.9
MsaG002071 MtrunA17_Chr5g0436501 53.030 66 29 1 37 100 126 191 2.69e-16 72.0
MsaG002071 MtrunA17_Chr4g0059701 54.688 64 29 0 37 100 85 148 1.76e-15 70.1
MsaG002071 MtrunA17_Chr5g0409831 48.611 72 34 1 38 106 79 150 6.62e-15 67.8
MsaG002071 MtrunA17_Chr6g0452791 45.455 77 36 2 10 85 58 129 9.69e-14 63.9
MsaG002071 MtrunA17_Chr8g0365011 43.902 82 32 2 38 105 94 175 2.66e-12 61.6
MsaG002071 MtrunA17_Chr3g0113121 65.909 44 15 0 38 81 97 140 2.99e-12 60.5
MsaG002071 MtrunA17_Chr5g0432921 56.863 51 21 1 32 82 109 158 9.18e-12 59.3
MsaG002071 MtrunA17_Chr5g0432941 54.902 51 22 1 32 82 109 158 5.87e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002071 AT5G56840.1 55.128 78 30 2 37 109 92 169 1.13e-20 83.6
MsaG002071 AT5G47390.1 52.941 68 32 0 37 104 97 164 7.69e-19 80.5
MsaG002071 AT5G61620.1 55.072 69 31 0 36 104 108 176 1.34e-18 79.3
MsaG002071 AT5G58900.1 53.521 71 30 1 37 104 142 212 1.91e-18 78.6
MsaG002071 AT5G04760.1 53.125 64 30 0 37 100 100 163 7.72e-18 75.9
MsaG002071 AT3G11280.2 44.444 90 48 1 13 100 102 191 7.72e-18 76.6
MsaG002071 AT3G11280.1 44.444 90 48 1 13 100 102 191 7.72e-18 76.6
MsaG002071 AT2G38090.1 53.125 64 30 0 37 100 142 205 1.72e-17 76.3
MsaG002071 AT5G01200.1 54.688 64 29 0 37 100 147 210 3.13e-17 75.1
MsaG002071 AT1G49010.1 47.143 70 37 0 37 106 135 204 3.31e-17 75.5
MsaG002071 AT5G08520.1 52.381 63 30 0 38 100 120 182 1.14e-16 73.9
MsaG002071 AT1G70000.1 56.250 64 28 0 37 100 99 162 1.29e-16 73.2
MsaG002071 AT1G70000.2 56.250 64 28 0 37 100 99 162 1.29e-16 73.2
MsaG002071 AT1G19000.2 55.224 67 30 0 37 103 103 169 2.41e-16 72.8
MsaG002071 AT1G19000.1 55.224 67 30 0 37 103 103 169 2.41e-16 72.8
MsaG002071 AT5G05790.2 51.562 64 31 0 37 100 132 195 4.21e-16 72.0
MsaG002071 AT5G05790.1 51.562 64 31 0 37 100 132 195 4.21e-16 72.0
MsaG002071 AT3G16350.1 51.471 68 33 0 37 104 138 205 8.89e-16 72.0
MsaG002071 AT5G23650.1 48.571 70 36 0 37 106 121 190 1.12e-15 71.6
MsaG002071 AT1G74840.2 57.812 64 27 0 37 100 97 160 1.16e-15 70.5
MsaG002071 AT1G74840.1 57.812 64 27 0 37 100 97 160 1.63e-15 70.5
MsaG002071 AT4G09450.1 43.750 80 45 0 37 116 92 171 8.18e-15 67.4
MsaG002071 AT3G10580.2 47.887 71 35 1 37 107 96 164 1.90e-14 67.4
MsaG002071 AT3G10590.1 47.436 78 36 2 37 112 112 186 2.00e-14 66.6
MsaG002071 AT3G10580.1 47.887 71 35 1 37 107 96 164 2.57e-14 67.4

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTTCGGGGAGGATATA+TGG 0.165012 1:-31717513 MsaT002071.1:CDS
GGCTTTCTTGAAAGGTTTAA+AGG 0.205247 1:-31715445 MsaT002071.1:CDS
GTGGGATGTCAACTTCCTTC+GGG 0.362116 1:-31717525 MsaT002071.1:CDS
TTCCTTCGGGGAGGATATAT+GGG 0.400096 1:-31717512 MsaT002071.1:CDS
GTGCCACCTCCCCTTCAGAC+TGG 0.418497 1:-31717560 MsaT002071.1:CDS
GGTTTAAAGGCTGTTGGAAA+AGG 0.435483 1:-31715432 MsaT002071.1:CDS
TTGAAAGGTTTAAAGGCTGT+TGG 0.453203 1:-31715438 MsaT002071.1:CDS
GGTGGGATGTCAACTTCCTT+CGG 0.453466 1:-31717526 MsaT002071.1:CDS
TGGTGCTCCGTCCTTGTCCA+TGG 0.462848 1:+31715468 None:intergenic
TTAAACCTTTCAAGAAAGCC+TGG 0.468661 1:+31715448 None:intergenic
GAGCACCAGGCTTTCTTGAA+AGG 0.474105 1:-31715453 MsaT002071.1:CDS
ATGGACAAGGACGGAGCACC+AGG 0.487460 1:-31715466 MsaT002071.1:CDS
GAAGAAAATGAAAATTCAAA+AGG 0.495823 1:-31715176 MsaT002071.1:CDS
TAGTGTAACACACACGCAAA+AGG 0.496000 1:+31717479 None:intergenic
AGGCTGTTGGAAAAGGCAAA+TGG 0.496236 1:-31715425 MsaT002071.1:CDS
GGAGGATATATGGGTTGGTC+AGG 0.505477 1:-31717503 MsaT002071.1:CDS
AATGAAAATTCAAAAGGTGC+TGG 0.512395 1:-31715170 MsaT002071.1:CDS
AAACCAGTCTGAAGGGGAGG+TGG 0.533352 1:+31717557 None:intergenic
TGGGATGTCAACTTCCTTCG+GGG 0.545743 1:-31717524 MsaT002071.1:CDS
AGCAACCTGAGTAGGAGTCT+TGG 0.550878 1:+31715374 None:intergenic
TTCGGGGAGGATATATGGGT+TGG 0.553643 1:-31717508 MsaT002071.1:CDS
GGTGACCAAGACTCCTACTC+AGG 0.568809 1:-31715379 MsaT002071.1:CDS
TTTGCATTATAGTGAAACCA+TGG 0.571448 1:-31715485 MsaT002071.1:intron
CTGGTGCTTCATCTGTATAG+AGG 0.574360 1:-31715151 None:intergenic
CAAATGGAAGCGAATATCGA+AGG 0.631038 1:-31715409 MsaT002071.1:CDS
GCATGACTAGCAACCTGAGT+AGG 0.641227 1:+31715366 None:intergenic
AGTGAAACCATGGACAAGGA+CGG 0.651405 1:-31715475 MsaT002071.1:CDS
AACCCATATATCCTCCCCGA+AGG 0.653717 1:+31717510 None:intergenic
GATGTCAACTTCCTTCGGGG+AGG 0.697160 1:-31717521 MsaT002071.1:CDS
TTATAGTGAAACCATGGACA+AGG 0.698291 1:-31715479 MsaT002071.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 31715154 31717588 31715154 ID=MsaG002071
Chr1 mRNA 31715154 31717588 31715154 ID=MsaT002071.1;Parent=MsaG002071
Chr1 exon 31715154 31715198 31715154 ID=MsaT002071.1.exon4;Parent=MsaT002071.1
Chr1 CDS 31715154 31715198 31715154 ID=cds.MsaT002071.1;Parent=MsaT002071.1
Chr1 exon 31715293 31715495 31715293 ID=MsaT002071.1.exon3;Parent=MsaT002071.1
Chr1 CDS 31715293 31715495 31715293 ID=cds.MsaT002071.1;Parent=MsaT002071.1
Chr1 exon 31715783 31715786 31715783 ID=MsaT002071.1.exon2;Parent=MsaT002071.1
Chr1 CDS 31715783 31715786 31715783 ID=cds.MsaT002071.1;Parent=MsaT002071.1
Chr1 exon 31717490 31717588 31717490 ID=MsaT002071.1.exon1;Parent=MsaT002071.1
Chr1 CDS 31717490 31717588 31717490 ID=cds.MsaT002071.1;Parent=MsaT002071.1
Gene Sequence

>MsaT002071.1

GATTTGGTGCCACCTCCCCTTCAGACTGGTTTTTGGATGGGGTGGGATGTCAACTTCCTTCGGGGAGGATATATGGGTTGGTCAGGTCCTTTTGCGTGTTCTCTGAAACCATGGACAAGGACGGAGCACCAGGCTTTCTTGAAAGGTTTAAAGGCTGTTGGAAAAGGCAAATGGAAGCGAATATCGAAGGATTTTGTGGTGACCAAGACTCCTACTCAGGTTGCTAGTCATGCACAAAAGCATTTTAATTACAGACGTGGTACATATAATAACAAAAATCGTACAAGCGTCTTTGACATGAAACTTGAAGAAAATGAAAATTCAAAAGGTGCTGGTGCTTCATCTGTATAG
Protein sequence

>MsaT002071.1

DLVPPPLQTGFWMGWDVNFLRGGYMGWSGPFACSLKPWTRTEHQAFLKGLKAVGKGKWKRISKDFVVTKTPTQVASHAQKHFNYRRGTYNNKNRTSVFDMKLEENENSKGAGASSV*