AlfalfaGEDB | MsaG002077

M. sativa ssp. caerulea / MsaG002077

KEGG: -
GO: GO:0001101 GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009719 GO:0009725 GO:0009739 GO:0009743 GO:0009744 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033993 GO:0034285 GO:0042221 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002077	XP_024630705.1	73.684	152	11	1	1	152	1	123	8.72e-72	221

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002077	sp\|Q9FKF9\|M5162_ARATH	42.759	145	65	5	16	142	34	178	3.86e-23	95.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002077	tr\|A0A072VIR9\|A0A072VIR9_MEDTR	96.341	82	3	0	71	152	11	92	1.58e-50	166

TFs/TRs:

Gene ID	Type	Classification
MsaG002077	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002077	MtrunA17_Chr4g0055931	45.833	96	50	1	41	136	66	159	6.45e-22	89.4
MsaG002077	MtrunA17_Chr8g0389241	54.118	85	37	2	69	152	102	185	7.89e-22	88.6
MsaG002077	MtrunA17_Chr2g0331031	44.086	93	51	1	45	136	81	173	5.61e-20	84.3
MsaG002077	MtrunA17_Chr6g0488471	57.971	69	27	2	69	136	94	161	1.37e-19	82.8
MsaG002077	MtrunA17_Chr3g0135671	70.000	50	15	0	69	118	88	137	2.89e-19	80.9
MsaG002077	MtrunA17_Chr5g0440741	47.872	94	46	1	52	142	80	173	3.11e-19	81.6
MsaG002077	MtrunA17_Chr5g0415191	35.099	151	80	4	4	136	16	166	3.73e-19	81.6
MsaG002077	MtrunA17_Chr5g0440731	56.944	72	28	1	74	142	107	178	3.93e-19	81.6
MsaG002077	MtrunA17_Chr7g0241761	46.988	83	44	0	69	151	137	219	1.11e-18	80.5
MsaG002077	MtrunA17_Chr5g0429521	54.412	68	31	0	69	136	129	196	1.52e-18	80.1
MsaG002077	MtrunA17_Chr3g0104071	54.412	68	31	0	69	136	144	211	1.94e-18	80.1
MsaG002077	MtrunA17_Chr5g0409841	51.429	70	32	1	74	141	94	163	1.01e-17	76.6
MsaG002077	MtrunA17_Chr1g0210471	51.471	68	33	0	69	136	121	188	1.54e-17	76.3
MsaG002077	MtrunA17_Chr4g0059701	37.681	138	65	4	17	136	14	148	5.41e-17	75.5
MsaG002077	MtrunA17_Chr4g0062741	48.529	68	35	0	69	136	135	202	6.03e-17	75.5
MsaG002077	MtrunA17_Chr5g0436501	41.509	106	57	3	33	136	89	191	7.86e-17	75.1
MsaG002077	MtrunA17_Chr3g0092621	50.000	64	32	0	73	136	85	148	2.38e-16	72.0
MsaG002077	MtrunA17_Chr1g0162081	52.381	63	30	0	74	136	124	186	2.75e-16	73.9
MsaG002077	MtrunA17_Chr5g0409831	64.444	45	16	0	74	118	79	123	4.14e-15	69.7
MsaG002077	MtrunA17_Chr6g0452791	57.143	49	21	0	73	121	81	129	2.60e-13	63.9
MsaG002077	MtrunA17_Chr3g0113121	65.909	44	15	0	74	117	97	140	1.44e-12	62.8
MsaG002077	MtrunA17_Chr5g0432921	60.784	51	19	1	68	118	109	158	2.90e-12	62.0
MsaG002077	MtrunA17_Chr8g0365011	43.902	82	32	2	74	141	94	175	2.42e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002077	AT5G61620.1	42.759	145	65	5	16	142	34	178	3.93e-24	95.5
MsaG002077	AT1G70000.1	39.850	133	68	3	12	136	34	162	4.97e-22	89.0
MsaG002077	AT1G70000.2	39.850	133	68	3	12	136	34	162	4.97e-22	89.0
MsaG002077	AT5G47390.1	37.500	152	64	4	13	140	20	164	5.01e-22	90.5
MsaG002077	AT1G74840.2	37.410	139	71	1	14	136	22	160	1.69e-21	87.0
MsaG002077	AT5G56840.1	36.478	159	87	3	1	145	11	169	2.71e-21	86.7
MsaG002077	AT1G74840.1	37.410	139	71	1	14	136	22	160	2.93e-21	87.0
MsaG002077	AT5G58900.1	50.617	81	37	1	63	140	132	212	7.24e-19	80.9
MsaG002077	AT5G04760.1	51.471	68	33	0	69	136	96	163	8.57e-18	77.0
MsaG002077	AT3G11280.2	52.941	68	32	0	69	136	124	191	9.75e-18	77.8
MsaG002077	AT3G11280.1	52.941	68	32	0	69	136	124	191	9.75e-18	77.8
MsaG002077	AT2G38090.1	52.941	68	32	0	69	136	138	205	1.31e-17	77.8
MsaG002077	AT5G01200.1	54.412	68	31	0	69	136	143	210	1.83e-17	77.0
MsaG002077	AT1G19000.2	35.256	156	61	4	16	139	22	169	2.01e-17	77.0
MsaG002077	AT1G19000.1	35.256	156	61	4	16	139	22	169	2.01e-17	77.0
MsaG002077	AT1G49010.1	43.210	81	46	0	69	149	131	211	9.00e-17	75.9
MsaG002077	AT3G16350.1	42.857	91	52	0	50	140	115	205	1.01e-16	76.3
MsaG002077	AT5G05790.2	50.000	68	34	0	69	136	128	195	3.08e-16	73.9
MsaG002077	AT5G05790.1	50.000	68	34	0	69	136	128	195	3.08e-16	73.9
MsaG002077	AT5G08520.1	52.381	63	30	0	74	136	120	182	4.65e-16	73.6
MsaG002077	AT5G23650.1	47.887	71	37	0	73	143	121	191	2.87e-15	71.6
MsaG002077	AT3G10580.2	48.000	75	37	1	69	143	92	164	7.57e-15	69.7
MsaG002077	AT3G10580.1	48.000	75	37	1	69	143	92	164	8.44e-15	70.1
MsaG002077	AT4G09450.1	44.304	79	44	0	73	151	92	170	1.58e-14	68.2
MsaG002077	AT3G10590.1	47.436	78	36	2	73	148	112	186	2.05e-14	67.8

Find 26 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCTTTCTTGAAAGGTTTAA+AGG	0.205247	1:+31846410	MsaT002077.1:CDS
CAACCATGTACACGTGAAAA+AGG	0.250846	1:+31843377	MsaT002077.1:CDS
ATGTGGTCATCATCTCTAAT+AGG	0.394732	1:-31843267	None:intergenic
GATAGGTGTGTGATGTTGTT+TGG	0.400177	1:+31843225	MsaT002077.1:CDS
GGTTTAAAGGCTGTTGGAAA+AGG	0.435483	1:+31846423	MsaT002077.1:CDS
TTGAAAGGTTTAAAGGCTGT+TGG	0.453203	1:+31846417	MsaT002077.1:CDS
GAAAATCATGAATTTGATAC+TGG	0.454021	1:+31843345	MsaT002077.1:CDS
TGGTGCTCCGTCCTTGTCCA+TGG	0.462848	1:-31846387	None:intergenic
TTAAACCTTTCAAGAAAGCC+TGG	0.468661	1:-31846407	None:intergenic
GAGCACCAGGCTTTCTTGAA+AGG	0.474105	1:+31846402	MsaT002077.1:CDS
ATGGACAAGGACGGAGCACC+AGG	0.487460	1:+31846389	MsaT002077.1:CDS
GAAGAAAATGAAAATTCAAA+AGG	0.495823	1:+31846679	MsaT002077.1:CDS
AGGCTGTTGGAAAAGGCAAA+TGG	0.496236	1:+31846430	MsaT002077.1:CDS
AATGAAAATTCAAAAGGTGC+TGG	0.512395	1:+31846685	MsaT002077.1:CDS
CGTACGATGAGAATGGTGAT+AGG	0.529349	1:+31843208	MsaT002077.1:CDS
AGCAACCTGAGTAGGAGTCT+TGG	0.550878	1:-31846481	None:intergenic
CGTCTTTAAATTATTCGACA+TGG	0.554516	1:-31843187	None:intergenic
TTAAAGACGTACGATGAGAA+TGG	0.562378	1:+31843201	MsaT002077.1:CDS
GACTAAAACTCTTGTTCATG+TGG	0.564121	1:-31843284	None:intergenic
GGTGACCAAGACTCCTACTC+AGG	0.568809	1:+31846476	MsaT002077.1:CDS
TTTGCATTATAGTGAAACCA+TGG	0.571448	1:+31846370	MsaT002077.1:intron
CTGGTGCTTCATCTGTATAG+AGG	0.574360	1:+31846704	MsaT002077.1:CDS
CAAATGGAAGCGAATATCGA+AGG	0.631038	1:+31846446	MsaT002077.1:CDS
GCATGACTAGCAACCTGAGT+AGG	0.641227	1:-31846489	None:intergenic
AGTGAAACCATGGACAAGGA+CGG	0.651405	1:+31846380	MsaT002077.1:intron
TTATAGTGAAACCATGGACA+AGG	0.698291	1:+31846376	MsaT002077.1:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	31843189	31846723	31843189	ID=MsaG002077
Chr1	mRNA	31843189	31846723	31843189	ID=MsaT002077.1;Parent=MsaG002077
Chr1	exon	31843189	31843399	31843189	ID=MsaT002077.1.exon1;Parent=MsaT002077.1
Chr1	CDS	31843189	31843399	31843189	ID=cds.MsaT002077.1;Parent=MsaT002077.1
Chr1	exon	31846382	31846584	31846382	ID=MsaT002077.1.exon2;Parent=MsaT002077.1
Chr1	CDS	31846382	31846584	31846382	ID=cds.MsaT002077.1;Parent=MsaT002077.1
Chr1	exon	31846679	31846723	31846679	ID=MsaT002077.1.exon3;Parent=MsaT002077.1
Chr1	CDS	31846679	31846723	31846679	ID=cds.MsaT002077.1;Parent=MsaT002077.1

Gene Sequence

>MsaT002077.1

ATGTCGAATAATTTAAAGACGTACGATGAGAATGGTGATAGGTGTGTGATGTTGTTTGGTGTGAACATCGCAAAAAATCCTATTAGAGATGATGACCACATGAACAAGAGTTTTAGTCTGGAAAACTTCAATCATAGACCTGATGAGTATGAAAACGAAAATCATGAATTTGATACTGGTCAAAGTTCCAACCATGTACACGTGAAAAAGGTGAAACCATGGACAAGGACGGAGCACCAGGCTTTCTTGAAAGGTTTAAAGGCTGTTGGAAAAGGCAAATGGAAGCGAATATCGAAGGATTTTGTGGTGACCAAGACTCCTACTCAGGTTGCTAGTCATGCACAAAAGCATTTTAATTACAGACGTGGTACATATAATAACAAAAATCGTACAAGCGTCTTTGACATGAAACTTGAAGAAAATGAAAATTCAAAAGGTGCTGGTGCTTCATCTGTATAG

Protein sequence

>MsaT002077.1

MSNNLKTYDENGDRCVMLFGVNIAKNPIRDDDHMNKSFSLENFNHRPDEYENENHEFDTGQSSNHVHVKKVKPWTRTEHQAFLKGLKAVGKGKWKRISKDFVVTKTPTQVASHAQKHFNYRRGTYNNKNRTSVFDMKLEENENSKGAGASSV*