AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002087

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002087 MtrunA17_Chr1g0170651 89.964 558 32 1 1 558 14 547 0.0 996
MsaG002087 MtrunA17_Chr5g0399801 39.609 563 258 11 28 557 576 1089 1.86e-113 362
MsaG002087 MtrunA17_Chr4g0003501 38.779 557 261 11 32 557 448 955 6.47e-109 347
MsaG002087 MtrunA17_Chr4g0003511 38.819 559 260 11 32 557 543 1052 1.09e-107 346
MsaG002087 MtrunA17_Chr7g0255181 32.117 548 297 16 34 554 203 702 1.77e-72 245
MsaG002087 MtrunA17_Chr8g0354821 31.150 565 299 10 34 554 167 685 6.34e-72 243
MsaG002087 MtrunA17_Chr3g0080761 33.205 518 255 15 85 556 191 663 1.48e-71 241
MsaG002087 MtrunA17_Chr6g0476121 31.760 551 286 15 56 557 134 643 9.28e-71 239
MsaG002087 MtrunA17_Chr6g0476111 31.579 551 287 15 56 557 185 694 4.85e-70 238
MsaG002087 MtrunA17_Chr7g0252211 31.444 547 276 15 65 557 256 757 7.18e-69 236
MsaG002087 MtrunA17_Chr7g0232811 30.339 590 249 16 18 557 62 539 2.13e-64 219
MsaG002087 MtrunA17_Chr7g0232981 30.756 582 247 17 9 557 58 516 9.25e-64 217
MsaG002087 MtrunA17_Chr8g0368911 31.000 500 270 14 67 531 289 748 1.39e-63 222
MsaG002087 MtrunA17_Chr1g0163051 31.380 529 281 13 44 531 157 644 1.55e-63 220
MsaG002087 MtrunA17_Chr8g0368901 32.186 494 260 13 73 531 302 755 1.31e-61 216
MsaG002087 MtrunA17_Chr8g0369301 31.944 504 266 15 64 531 273 735 2.21e-61 216
MsaG002087 MtrunA17_Chr6g0467381 31.762 488 234 15 88 531 130 562 2.07e-56 198
MsaG002087 MtrunA17_Chr6g0467421 31.557 488 235 15 88 531 130 562 2.84e-56 198
MsaG002087 MtrunA17_Chr6g0467531 31.967 488 233 15 88 531 130 562 3.28e-56 198
MsaG002087 MtrunA17_Chr6g0467401 31.677 483 241 15 88 531 130 562 8.11e-55 194
MsaG002087 MtrunA17_Chr1g0163041 30.966 507 262 17 66 531 104 563 2.30e-53 191
MsaG002087 MtrunA17_Chr7g0232841 29.244 595 235 22 18 557 65 528 1.52e-51 184
MsaG002087 MtrunA17_Chr6g0467411 29.193 483 232 16 88 531 69 480 2.75e-46 169
MsaG002087 MtrunA17_Chr3g0094881 28.243 478 262 13 88 531 191 621 1.34e-44 167
MsaG002087 MtrunA17_Chr2g0306961 28.931 477 212 16 88 531 79 461 1.51e-40 153
MsaG002087 MtrunA17_Chr5g0403241 31.778 343 178 10 268 557 1168 1507 1.77e-34 139
MsaG002087 MtrunA17_Chr8g0373821 32.313 294 154 9 299 552 245 533 7.74e-33 132
MsaG002087 MtrunA17_Chr4g0075321 37.811 201 104 6 373 557 869 1064 2.45e-30 126
MsaG002087 MtrunA17_Chr1g0183321 29.299 314 157 10 304 552 428 741 3.51e-26 113
MsaG002087 MtrunA17_Chr1g0190311 29.693 293 140 10 304 531 325 616 6.00e-25 109
MsaG002087 MtrunA17_Chr7g0262001 25.750 400 191 15 244 552 313 697 4.59e-20 94.4
MsaG002087 MtrunA17_Chr7g0232751 43.478 92 47 1 471 557 28 119 9.84e-20 85.1
MsaG002087 MtrunA17_Chr1g0201311 36.000 175 84 7 392 553 61 220 4.63e-19 90.1
MsaG002087 MtrunA17_Chr1g0201621 35.429 175 85 7 392 553 61 220 2.39e-18 88.2
MsaG002087 MtrunA17_Chr6g0460921 29.075 227 123 8 347 556 214 419 2.19e-17 84.7
MsaG002087 MtrunA17_Chr3g0125251 33.333 168 96 4 396 556 1306 1464 5.23e-16 82.0
MsaG002087 MtrunA17_Chr7g0232861 34.225 187 99 8 18 200 61 227 7.20e-16 77.4
MsaG002087 MtrunA17_Chr2g0305371 30.342 234 124 10 328 554 268 469 1.48e-15 79.3
MsaG002087 MtrunA17_Chr7g0232871 46.667 75 37 2 396 469 4 76 3.03e-14 69.3
MsaG002087 MtrunA17_Chr7g0232761 33.333 195 105 9 33 225 94 265 3.43e-14 73.2
MsaG002087 MtrunA17_Chr1g0149611 35.862 145 79 5 416 552 365 503 3.78e-14 75.1
MsaG002087 MtrunA17_Chr6g0477001 29.480 173 100 5 389 552 87 246 5.75e-14 73.6
MsaG002087 MtrunA17_Chr1g0149581 33.793 145 82 5 416 552 1523 1661 4.38e-12 69.3
MsaG002087 MtrunA17_Chr2g0297191 30.405 148 87 4 415 556 1033 1170 6.63e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002087 AT2G35160.5 40.449 534 262 15 34 557 271 758 4.66e-113 353
MsaG002087 AT2G35160.2 40.449 534 262 15 34 557 307 794 1.39e-112 353
MsaG002087 AT2G35160.3 40.449 534 262 15 34 557 307 794 1.39e-112 353
MsaG002087 AT2G35160.1 40.449 534 262 15 34 557 307 794 1.39e-112 353
MsaG002087 AT2G35160.4 40.449 534 262 15 34 557 307 794 1.39e-112 353
MsaG002087 AT2G22740.2 35.025 611 292 15 3 557 229 790 7.16e-95 306
MsaG002087 AT2G22740.1 35.025 611 292 15 3 557 229 790 7.16e-95 306
MsaG002087 AT1G73100.1 32.685 514 257 14 85 556 202 668 2.03e-70 239
MsaG002087 AT5G04940.2 33.008 512 256 14 85 556 205 669 1.35e-68 234
MsaG002087 AT5G04940.1 33.008 512 256 14 85 556 205 669 1.35e-68 234
MsaG002087 AT2G33290.1 31.569 548 295 15 34 547 149 650 6.15e-64 221
MsaG002087 AT2G33290.3 31.569 548 295 15 34 547 149 650 6.15e-64 221
MsaG002087 AT2G33290.4 31.569 548 295 15 34 547 149 650 6.15e-64 221
MsaG002087 AT2G33290.2 31.569 548 295 15 34 547 149 650 6.15e-64 221
MsaG002087 AT5G13960.2 31.214 519 268 16 88 557 12 490 2.57e-61 210
MsaG002087 AT5G13960.1 30.823 571 296 19 36 557 104 624 1.28e-60 211
MsaG002087 AT4G13460.1 30.241 539 288 15 34 531 145 636 4.26e-59 208
MsaG002087 AT4G13460.2 30.241 539 288 15 34 531 145 636 4.26e-59 208
MsaG002087 AT1G17770.1 30.174 517 271 17 85 556 221 692 1.15e-55 199
MsaG002087 AT2G24740.1 30.059 509 261 16 85 556 304 754 3.16e-51 187
MsaG002087 AT2G23740.3 33.439 314 163 10 280 554 1073 1379 4.18e-35 141
MsaG002087 AT2G23740.6 33.439 314 163 10 280 554 1073 1379 4.18e-35 141
MsaG002087 AT2G23740.2 33.439 314 163 10 280 554 1073 1379 4.18e-35 141
MsaG002087 AT2G23740.1 33.439 314 163 10 280 554 1066 1372 4.41e-35 141
MsaG002087 AT5G47150.1 38.889 216 121 4 34 247 118 324 2.99e-30 121
MsaG002087 AT5G47160.1 35.833 240 134 4 10 243 182 407 1.92e-27 115
MsaG002087 AT5G47160.3 35.833 240 134 4 10 243 182 407 1.92e-27 115
MsaG002087 AT5G47160.2 35.833 240 134 4 10 243 182 407 1.92e-27 115
MsaG002087 AT3G04380.3 26.744 430 213 19 201 552 36 441 1.08e-23 104
MsaG002087 AT3G04380.1 26.744 430 213 19 201 552 52 457 1.28e-23 104
MsaG002087 AT3G04380.4 26.744 430 213 19 201 552 52 457 1.57e-23 104
MsaG002087 AT3G04380.2 28.529 333 169 13 287 552 100 430 6.35e-23 102
MsaG002087 AT3G04380.6 28.529 333 169 13 287 552 100 430 6.55e-23 102
MsaG002087 AT3G04380.5 28.529 333 169 13 287 552 84 414 6.66e-23 102
MsaG002087 AT2G23740.4 32.530 249 123 9 280 490 1073 1314 8.39e-23 103
MsaG002087 AT2G23740.5 32.530 249 123 9 280 490 1073 1314 8.39e-23 103
MsaG002087 AT3G03750.2 33.696 184 100 4 392 556 169 349 6.56e-21 94.7
MsaG002087 AT5G43990.16 34.973 183 104 7 384 552 500 681 1.42e-20 96.3
MsaG002087 AT5G43990.5 34.973 183 104 7 384 552 500 681 1.42e-20 96.3
MsaG002087 AT5G43990.17 34.973 183 104 7 384 552 500 681 1.42e-20 96.3
MsaG002087 AT5G43990.14 34.973 183 104 7 384 552 500 681 1.42e-20 96.3
MsaG002087 AT5G43990.15 34.973 183 104 7 384 552 523 704 1.46e-20 96.3
MsaG002087 AT5G43990.10 34.973 183 104 7 384 552 523 704 1.46e-20 96.3
MsaG002087 AT5G43990.3 34.973 183 104 7 384 552 520 701 1.51e-20 96.3
MsaG002087 AT5G43990.11 34.973 183 104 7 384 552 520 701 1.51e-20 96.3
MsaG002087 AT5G43990.1 34.973 183 104 7 384 552 520 701 1.51e-20 96.3
MsaG002087 AT5G43990.4 34.973 183 104 7 384 552 520 701 1.51e-20 96.3
MsaG002087 AT5G43990.9 34.973 183 104 7 384 552 543 724 1.63e-20 96.3
MsaG002087 AT5G43990.2 34.973 183 104 7 384 552 543 724 1.63e-20 96.3
MsaG002087 AT5G43990.8 34.973 183 104 7 384 552 451 632 1.67e-20 95.9
MsaG002087 AT5G43990.12 34.973 183 104 7 384 552 451 632 1.67e-20 95.9
MsaG002087 AT5G43990.6 34.973 183 104 7 384 552 451 632 1.67e-20 95.9
MsaG002087 AT5G43990.13 34.973 183 104 7 384 552 451 632 1.67e-20 95.9
MsaG002087 AT5G43990.7 34.973 183 104 7 384 552 431 612 1.96e-20 95.5
MsaG002087 AT1G04050.2 32.447 188 109 6 384 555 536 721 1.11e-18 90.5
MsaG002087 AT1G04050.1 32.447 188 109 6 384 555 536 721 1.11e-18 90.5
MsaG002087 AT1G04050.3 32.447 188 109 6 384 555 432 617 1.31e-18 90.1
MsaG002087 AT1G77300.1 30.952 168 100 4 396 556 1008 1166 1.74e-15 80.9
MsaG002087 AT1G77300.2 30.952 168 100 4 396 556 1008 1166 1.82e-15 80.5
MsaG002087 AT3G03750.1 33.728 169 104 4 392 556 169 333 2.46e-15 77.8
MsaG002087 AT4G30860.1 28.631 241 130 8 321 556 261 464 1.42e-13 73.6
MsaG002087 AT1G76710.2 31.395 172 98 7 392 554 66 226 2.48e-13 72.8
MsaG002087 AT1G76710.1 31.395 172 98 7 392 554 66 226 2.48e-13 72.8
MsaG002087 AT1G76710.4 31.395 172 98 7 392 554 66 226 2.71e-13 72.8
MsaG002087 AT1G76710.3 31.395 172 98 7 392 554 66 226 2.83e-13 72.8
MsaG002087 AT3G59960.3 30.539 167 94 4 399 556 96 249 5.67e-11 64.3
MsaG002087 AT3G59960.2 30.539 167 94 4 399 556 96 249 5.67e-11 64.3
MsaG002087 AT3G59960.4 30.539 167 94 4 399 556 96 249 5.67e-11 64.3
MsaG002087 AT5G42400.7 30.201 149 86 5 415 556 1250 1387 6.46e-11 65.9
MsaG002087 AT5G42400.2 30.201 149 86 5 415 556 1250 1387 6.46e-11 65.9
MsaG002087 AT5G42400.8 30.201 149 86 5 415 556 1250 1387 6.46e-11 65.9
MsaG002087 AT5G42400.5 30.201 149 86 5 415 556 1250 1387 6.46e-11 65.9
MsaG002087 AT5G42400.4 30.201 149 86 5 415 556 1250 1387 6.46e-11 65.9
MsaG002087 AT5G42400.1 30.201 149 86 5 415 556 1250 1387 6.46e-11 65.9
MsaG002087 AT5G42400.3 30.201 149 86 5 415 556 1250 1387 6.46e-11 65.9
MsaG002087 AT5G42400.6 30.201 149 86 5 415 556 1250 1387 6.46e-11 65.9

Find 131 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATGTGGTATCGATTATT+TGG 0.102559 1:+32064479 MsaT002087.1:CDS
TACATGCCCTATTCTCTTTC+CGG 0.210201 1:-32064385 None:intergenic
ATGCAAAGTTCAAAATCTTT+TGG 0.219974 1:+32064129 MsaT002087.1:CDS
TAAAATTGAAAGCAAAATTA+AGG 0.224504 1:+32064310 MsaT002087.1:CDS
GCGACACCGTCCCTTATATT+AGG 0.242119 1:-32064606 None:intergenic
CTTCAGAATTGTGTCTCTTC+TGG 0.280628 1:-32064908 None:intergenic
GGTATCTCCACATAGCTTAT+AGG 0.280802 1:-32065089 None:intergenic
TCCTGTTAGGGTTATATTGC+AGG 0.288637 1:+32064692 MsaT002087.1:CDS
GTTTGTGCTTAAGAAAATTC+AGG 0.305686 1:+32064842 MsaT002087.1:CDS
GTTCGGCCCTATATTGAAAT+TGG 0.307938 1:-32064428 None:intergenic
AGTATTGAACGATTCGTCTT+TGG 0.310118 1:-32065146 None:intergenic
GTATCGATTATTTGGGTAAA+GGG 0.327727 1:+32064487 MsaT002087.1:CDS
TGTTCGAATCAATGATATTT+CGG 0.328396 1:+32065031 MsaT002087.1:CDS
ACTAGGTAACCGAAGGGTTT+AGG 0.329178 1:-32065110 None:intergenic
TGTTTCCCCTCGAGATGTTA+TGG 0.333335 1:+32064878 MsaT002087.1:CDS
CGCGCCTCATGATCAGAAAT+TGG 0.347176 1:+32064626 MsaT002087.1:CDS
ACTCGAGTAAGAGGACAATT+TGG 0.352436 1:+32064804 MsaT002087.1:CDS
TGGTGACCAATTTCAATATA+GGG 0.354159 1:+32064422 MsaT002087.1:CDS
CGCGCGGAAATCTTGCTTTG+TGG 0.366478 1:+32064649 MsaT002087.1:CDS
ATATAAAGGATGATTTCATT+TGG 0.370033 1:-32065277 None:intergenic
ATGGTGGTAACCCTAATATA+AGG 0.370183 1:+32064595 MsaT002087.1:CDS
ATAGGTGTCACCACCCGAAT+AGG 0.370547 1:-32065071 None:intergenic
TATAAAGGATGATTTCATTT+GGG 0.376053 1:-32065276 None:intergenic
ACCTGCAATATAACCCTAAC+AGG 0.380684 1:-32064693 None:intergenic
GGGTAAAGGGGAGGGTTCTT+TGG 0.380696 1:+32064500 MsaT002087.1:CDS
TCTGGTTGTTTGCTTTATGA+AGG 0.381509 1:+32064570 MsaT002087.1:CDS
AATTTGCTACTGTGGTTCAT+TGG 0.383046 1:+32065846 MsaT002087.1:CDS
TGAATGCGGGGAGTCCTGTT+AGG 0.386175 1:+32064679 MsaT002087.1:CDS
GCAAAGAAAAGTTCCCTATT+CGG 0.386328 1:+32065057 MsaT002087.1:CDS
CGACACCGTCCCTTATATTA+GGG 0.395566 1:-32064605 None:intergenic
TCTTATTCATACATATATGA+TGG 0.403540 1:+32064759 MsaT002087.1:CDS
TTGGTGACCAATTTCAATAT+AGG 0.405625 1:+32064421 MsaT002087.1:CDS
CAAAGAAAAGTTCCCTATTC+GGG 0.406699 1:+32065058 MsaT002087.1:CDS
GTTTCCCCTCGAGATGTTAT+GGG 0.409974 1:+32064879 MsaT002087.1:CDS
GCGAATGTTATGAAGTCTTC+TGG 0.411419 1:+32064552 MsaT002087.1:CDS
AAAATTGAAAGCAAAATTAA+GGG 0.414139 1:+32064311 MsaT002087.1:CDS
GATGAGTATACAATTAATAT+AGG 0.418381 1:+32065512 MsaT002087.1:CDS
AGGGCCACACTCATATATAA+AGG 0.421860 1:-32065291 None:intergenic
CTTCATAAACCTTGTAGCTT+CGG 0.427305 1:-32064340 None:intergenic
TCATGTGGTATCGATTATTT+GGG 0.431263 1:+32064480 MsaT002087.1:CDS
AAGACGAATCGTTCAATACT+AGG 0.431282 1:+32065149 MsaT002087.1:CDS
ACATCTTTCACAACTAGGTT+AGG 0.431941 1:-32065697 None:intergenic
CAAAATGTTGTTTGCTACAA+AGG 0.440548 1:+32065753 MsaT002087.1:CDS
GAGCTCACACACAAAGCTTC+CGG 0.447492 1:-32065435 None:intergenic
GAAAACACCGGAAAGAGAAT+AGG 0.449177 1:+32064378 MsaT002087.1:CDS
AGGGCCGAACTTATTGTAAT+CGG 0.452930 1:+32064441 MsaT002087.1:CDS
AAAACACCGGAAAGAGAATA+GGG 0.465297 1:+32064379 MsaT002087.1:CDS
GTGTTTGCATAGCTAAGAAT+GGG 0.467332 1:+32065216 MsaT002087.1:CDS
GATTATTTGGGTAAAGGGGA+GGG 0.467741 1:+32064492 MsaT002087.1:CDS
AATCTTCAAAACAAAATTGA+AGG 0.468829 1:+32065379 MsaT002087.1:CDS
AAGAGAATAGGGCATGTAGA+TGG 0.472138 1:+32064390 MsaT002087.1:CDS
GGTATCGATTATTTGGGTAA+AGG 0.478346 1:+32064486 MsaT002087.1:CDS
GACAATGTGACTCGAGTAAG+AGG 0.478502 1:+32064795 MsaT002087.1:CDS
TTATGAAGGCGAAACGTCAA+TGG 0.484134 1:+32064355 MsaT002087.1:CDS
TTCAAGTTGGAACTGAATAC+CGG 0.487311 1:-32065357 None:intergenic
TAGGTGTCACCACCCGAATA+GGG 0.488457 1:-32065070 None:intergenic
TACCACATGAGTGTTGTTGA+TGG 0.494297 1:-32064467 None:intergenic
TAAGAAAATTCAGGACCAAC+CGG 0.497017 1:+32064851 MsaT002087.1:CDS
CTTATTCATACATATATGAT+GGG 0.498680 1:+32064760 MsaT002087.1:CDS
ATTGATTCGAACAACATCCT+TGG 0.502629 1:-32065021 None:intergenic
CGATTATTTGGGTAAAGGGG+AGG 0.503266 1:+32064491 MsaT002087.1:CDS
AGCTTTGTGTGTGAGCTCAT+TGG 0.503294 1:+32065440 MsaT002087.1:CDS
TTCATTGATGCAACGCGACA+TGG 0.503420 1:+32065640 MsaT002087.1:CDS
ACATAACATCTTTCACAACT+AGG 0.508844 1:-32065702 None:intergenic
CAAAACAAAATTGAAGGGGT+GGG 0.513316 1:+32065385 MsaT002087.1:CDS
GGGCTTTAGCCGAAGCTACA+AGG 0.516710 1:+32064331 MsaT002087.1:CDS
ATGGACAAATCTACATTTGA+AGG 0.517049 1:+32065875 MsaT002087.1:CDS
ACGAGGAACACAAGCAAGAA+GGG 0.521209 1:-32065310 None:intergenic
ATCAGCCGAGTGAGTCAATC+CGG 0.522206 1:+32065338 MsaT002087.1:CDS
TTGGGAGAAAGCGTGAAGAA+TGG 0.523029 1:+32064148 MsaT002087.1:CDS
GACGAGGAACACAAGCAAGA+AGG 0.525389 1:-32065311 None:intergenic
ACACGCTCTTTCATTCCATC+CGG 0.526296 1:+32065416 MsaT002087.1:CDS
TGGTGGTAACCCTAATATAA+GGG 0.526528 1:+32064596 MsaT002087.1:CDS
AAACATTCTTTGGGAGAACA+TGG 0.531170 1:-32064932 None:intergenic
CATGTAGATGGAATTGAAGT+TGG 0.532327 1:+32064402 MsaT002087.1:CDS
TTGAGTAAAATTTGCTACTG+TGG 0.533623 1:+32065838 MsaT002087.1:CDS
GAATGCGGGGAGTCCTGTTA+GGG 0.541817 1:+32064680 MsaT002087.1:CDS
GAGACCGATTACAATAAGTT+CGG 0.543201 1:-32064445 None:intergenic
GCAACGCGACATGGAAACAT+TGG 0.543624 1:+32065649 MsaT002087.1:CDS
TCAAAACAAAATTGAAGGGG+TGG 0.543766 1:+32065384 MsaT002087.1:CDS
CGAAGCTACAAGGTTTATGA+AGG 0.543860 1:+32064341 MsaT002087.1:CDS
ATCTTCAAAACAAAATTGAA+GGG 0.544882 1:+32065380 MsaT002087.1:CDS
CGCGCCAATTTCTGATCATG+AGG 0.548143 1:-32064630 None:intergenic
TATGAAGGCGAAACGTCAAT+GGG 0.552062 1:+32064356 MsaT002087.1:CDS
AAAACAAAATTGAAGGGGTG+GGG 0.557230 1:+32065386 MsaT002087.1:CDS
TGTGTTTGCATAGCTAAGAA+TGG 0.557259 1:+32065215 MsaT002087.1:CDS
TAGAGGTCTTGTTCTCTCCA+AGG 0.558562 1:+32065004 MsaT002087.1:CDS
TCTATGAGTTACAACAATGC+TGG 0.561265 1:-32064526 None:intergenic
GTGGAGATACCTAAACCCTT+CGG 0.568688 1:+32065101 MsaT002087.1:CDS
TTATCTTGTCAGAAGATGAA+AGG 0.571853 1:-32064185 None:intergenic
CGCGACATGGAAACATTGGA+AGG 0.575552 1:+32065653 MsaT002087.1:CDS
TGTTTGCTTTATGAAGGTCA+TGG 0.576682 1:+32064576 MsaT002087.1:CDS
TTGGTTTCCATAACATCTCG+AGG 0.577098 1:-32064957 None:intergenic
CGAATCAATGATATTTCGGA+GGG 0.577253 1:+32065035 MsaT002087.1:CDS
CAGAAGAGACACAATTCTGA+AGG 0.581158 1:+32064909 MsaT002087.1:CDS
TTGCTGCAAGGTGAACATCA+AGG 0.582980 1:+32065813 MsaT002087.1:CDS
TAACTCAGCTCTCTACCTGC+GGG 0.583390 1:-32065781 None:intergenic
AATCGTTCAATACTAGGTGG+TGG 0.591568 1:+32065155 MsaT002087.1:CDS
TTGTGGAATAGTATGAATGC+GGG 0.595742 1:+32064666 MsaT002087.1:CDS
CATATTGACTAGGTAACCGA+AGG 0.601280 1:-32065117 None:intergenic
TCTCGAGGGGAAACACTAGC+CGG 0.615076 1:-32064870 None:intergenic
TGTGGTTCATTGGAATGCAA+TGG 0.618637 1:+32065856 MsaT002087.1:CDS
TTGGATAAGTCATATTGACT+AGG 0.621126 1:-32065127 None:intergenic
AAAGTTCCCATAACATCTCG+AGG 0.623289 1:-32064885 None:intergenic
TATCGATTATTTGGGTAAAG+GGG 0.626149 1:+32064488 MsaT002087.1:CDS
TCTTCAAAACAAAATTGAAG+GGG 0.626316 1:+32065381 MsaT002087.1:CDS
TCGAATCAATGATATTTCGG+AGG 0.627900 1:+32065034 MsaT002087.1:CDS
GAGGGGAAACACTAGCCGGT+TGG 0.631704 1:-32064866 None:intergenic
ATAACTCAGCTCTCTACCTG+CGG 0.634593 1:-32065782 None:intergenic
CACTCGGCTGATGCAAGACG+AGG 0.637041 1:-32065327 None:intergenic
AAGTTCCCATAACATCTCGA+GGG 0.638178 1:-32064884 None:intergenic
TCATCCTTTATATATGAGTG+TGG 0.641509 1:+32065287 MsaT002087.1:CDS
GGTGACACCTATAAGCTATG+TGG 0.646281 1:+32065082 MsaT002087.1:CDS
GCTACAAAGGACATACCCGC+AGG 0.652394 1:+32065766 MsaT002087.1:CDS
TGTTTGCATAGCTAAGAATG+GGG 0.664275 1:+32065217 MsaT002087.1:CDS
GGTAACCCTAATATAAGGGA+CGG 0.664705 1:+32064600 MsaT002087.1:CDS
CTCCATCAACAACACTCATG+TGG 0.665071 1:+32064465 MsaT002087.1:CDS
GAATACCGGATTGACTCACT+CGG 0.672661 1:-32065343 None:intergenic
AGTTCCCATAACATCTCGAG+GGG 0.674068 1:-32064883 None:intergenic
TTTGCATAGCTAAGAATGGG+GGG 0.675484 1:+32065219 MsaT002087.1:CDS
ATATTGACTAGGTAACCGAA+GGG 0.676746 1:-32065116 None:intergenic
TCACACACAAAGCTTCCGGA+TGG 0.681981 1:-32065431 None:intergenic
AGAAAAGTTCCCTATTCGGG+TGG 0.682829 1:+32065061 MsaT002087.1:CDS
AAACGTCAATGGGAAAACAC+CGG 0.685237 1:+32064366 MsaT002087.1:CDS
TGTGGAATAGTATGAATGCG+GGG 0.700340 1:+32064667 MsaT002087.1:CDS
CATGATCAGAAATTGGCGCG+CGG 0.700635 1:+32064633 MsaT002087.1:CDS
TTTGTGGAATAGTATGAATG+CGG 0.704358 1:+32064665 MsaT002087.1:CDS
TTGCTTTATGAAGGTCATGG+TGG 0.708605 1:+32064579 MsaT002087.1:CDS
GTTTGCATAGCTAAGAATGG+GGG 0.714167 1:+32065218 MsaT002087.1:CDS
TTATGATTACAATTGCTGCA+AGG 0.726923 1:+32065801 MsaT002087.1:CDS
ACGAATCGTTCAATACTAGG+TGG 0.736474 1:+32065152 MsaT002087.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 32064129 32065894 32064129 ID=MsaG002087
Chr1 mRNA 32064129 32065894 32064129 ID=MsaT002087.1;Parent=MsaG002087
Chr1 exon 32064129 32065533 32064129 ID=MsaT002087.1.exon1;Parent=MsaT002087.1
Chr1 CDS 32064129 32065533 32064129 ID=cds.MsaT002087.1;Parent=MsaT002087.1
Chr1 exon 32065623 32065894 32065623 ID=MsaT002087.1.exon2;Parent=MsaT002087.1
Chr1 CDS 32065623 32065894 32065623 ID=cds.MsaT002087.1;Parent=MsaT002087.1
Gene Sequence

>MsaT002087.1

ATGCAAAGTTCAAAATCTTTTGGGAGAAAGCGTGAAGAATGGTCATATACTGAGAACCTTTCATCTTCTGACAAGATAAGTAGTGACACTGTTTTGAAACGGAGAAAGGTGAATAAAGCATTAACAATTTTTAGGGACTTATCACACAAACTCTATTGTGAATACAAGTCAAACCAACCTGTAAAATTGAAAGCAAAATTAAGGGCTTTAGCCGAAGCTACAAGGTTTATGAAGGCGAAACGTCAATGGGAAAACACCGGAAAGAGAATAGGGCATGTAGATGGAATTGAAGTTGGTGACCAATTTCAATATAGGGCCGAACTTATTGTAATCGGTCTCCATCAACAACACTCATGTGGTATCGATTATTTGGGTAAAGGGGAGGGTTCTTTGGCAACCAGCATTGTTGTAACTCATAGATATGCGAATGTTATGAAGTCTTCTGGTTGTTTGCTTTATGAAGGTCATGGTGGTAACCCTAATATAAGGGACGGTGTCGCGCCTCATGATCAGAAATTGGCGCGCGGAAATCTTGCTTTGTGGAATAGTATGAATGCGGGGAGTCCTGTTAGGGTTATATTGCAGGTTTCTGAAAAATCTGAAGTGATGGAAGAGTTGAGCAACAATGTTTCTTATTCATACATATATGATGGGTTGTATTTTGTGGACAATGTGACTCGAGTAAGAGGACAATTTGGAAAACTTGTTTTCAAGTTTGTGCTTAAGAAAATTCAGGACCAACCGGCTAGTGTTTCCCCTCGAGATGTTATGGGAACTTTCCAGAAGAGACACAATTCTGAAGGCCATGTTCTCCCAAAGAATGTTTTTCCTCGAGATGTTATGGAAACCAAAACTTTCCGAAAAAGACAAATGTCTAGAGGTCTTGTTCTCTCCAAGGATGTTGTTCGAATCAATGATATTTCGGAGGGCAAAGAAAAGTTCCCTATTCGGGTGGTGACACCTATAAGCTATGTGGAGATACCTAAACCCTTCGGTTACCTAGTCAATATGACTTATCCAAAGACGAATCGTTCAATACTAGGTGGTGGATGTAATTGTAAGAATGCATGTGTTGATTGTCTTGAATGTGTTTGCATAGCTAAGAATGGGGGGACATTAGCCTATGATGGTAACAGAAAACTTGTTTCCCAAATGAAATCATCCTTTATATATGAGTGTGGCCCTTCTTGCTTGTGTTCCTCGTCTTGCATCAGCCGAGTGAGTCAATCCGGTATTCAGTTCCAACTTGAAATCTTCAAAACAAAATTGAAGGGGTGGGGTGTTCGAACACGCTCTTTCATTCCATCCGGAAGCTTTGTGTGTGAGCTCATTGGAGAAGTTGTTCATCTCAACAACAAAAAAGTTGGATCAAATCTACATGTTGATGAGTATACAATTAATATAGGTGCTTCTGGCAAGGTCTTCATTGATGCAACGCGACATGGAAACATTGGAAGGTTTATAAATCATAGTTGTTCTCCTAACCTAGTTGTGAAAGATGTTATGTATGATCACAGTGATAAGAGTTTACCCCACAAAATGTTGTTTGCTACAAAGGACATACCCGCAGGTAGAGAGCTGAGTTATGATTACAATTGCTGCAAGGTGAACATCAAGGTTTTGAGTAAAATTTGCTACTGTGGTTCATTGGAATGCAATGGACAAATCTACATTTGA
Protein sequence

>MsaT002087.1

MQSSKSFGRKREEWSYTENLSSSDKISSDTVLKRRKVNKALTIFRDLSHKLYCEYKSNQPVKLKAKLRALAEATRFMKAKRQWENTGKRIGHVDGIEVGDQFQYRAELIVIGLHQQHSCGIDYLGKGEGSLATSIVVTHRYANVMKSSGCLLYEGHGGNPNIRDGVAPHDQKLARGNLALWNSMNAGSPVRVILQVSEKSEVMEELSNNVSYSYIYDGLYFVDNVTRVRGQFGKLVFKFVLKKIQDQPASVSPRDVMGTFQKRHNSEGHVLPKNVFPRDVMETKTFRKRQMSRGLVLSKDVVRINDISEGKEKFPIRVVTPISYVEIPKPFGYLVNMTYPKTNRSILGGGCNCKNACVDCLECVCIAKNGGTLAYDGNRKLVSQMKSSFIYECGPSCLCSSSCISRVSQSGIQFQLEIFKTKLKGWGVRTRSFIPSGSFVCELIGEVVHLNNKKVGSNLHVDEYTINIGASGKVFIDATRHGNIGRFINHSCSPNLVVKDVMYDHSDKSLPHKMLFATKDIPAGRELSYDYNCCKVNIKVLSKICYCGSLECNGQIYI*