AlfalfaGEDB | MsaG002097

M. sativa ssp. caerulea / MsaG002097

KEGG: K14492
GO: GO:0000003 GO:0000156 GO:0000160 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009987 GO:0010017 GO:0010033 GO:0010114 GO:0010154 GO:0010161 GO:0010468 GO:0010556 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0023052 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048316 GO:0048511 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071214 GO:0071310 GO:0071368 GO:0071478 GO:0071482 GO:0071489 GO:0071491 GO:0071495 GO:0071704 GO:0080090 GO:0104004 GO:0140096 GO:1901564 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00072.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002097	RHN78877.1	88.889	198	18	1	1	194	1	198	1.56e-109	321

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002097	sp\|Q9ZWS9\|ARR3_ARATH	59.406	202	59	5	4	186	29	226	7.36e-71	217

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002097	tr\|A0A396JVZ4\|A0A396JVZ4_MEDTR	88.889	198	18	1	1	194	1	198	7.47e-110	321

TFs/TRs:

Gene ID	Type	Classification
MsaG002097	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG003532	MsaG002097	0.804874	2.959188e-49	4.473270e-47
MsaG010773	MsaG002097	0.859665	6.935552e-63	5.323924e-60
MsaG017721	MsaG002097	0.848103	1.434747e-59	7.290993e-57
MsaG018541	MsaG002097	0.860162	4.919979e-63	3.849370e-60
MsaG028110	MsaG002097	0.868402	1.355522e-65	1.470158e-62
MsaG030709	MsaG002097	0.806207	1.559276e-49	2.431783e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002097	MtrunA17_Chr1g0170741	88.889	198	18	1	1	194	1	198	1.44e-113	321
MsaG002097	MtrunA17_Chr5g0414931	66.667	237	36	4	1	194	1	237	5.82e-91	266
MsaG002097	MtrunA17_Chr4g0059571	61.538	169	62	1	3	171	21	186	4.46e-64	197
MsaG002097	MtrunA17_Chr3g0118211	61.006	159	56	2	7	162	22	177	8.89e-61	188
MsaG002097	MtrunA17_Chr7g0256551	62.319	138	46	1	7	138	8	145	1.46e-55	176
MsaG002097	MtrunA17_Chr3g0082861	56.129	155	59	2	7	152	3	157	8.96e-53	166
MsaG002097	MtrunA17_Chr8g0352871	59.155	142	53	2	7	143	8	149	4.40e-51	162
MsaG002097	MtrunA17_Chr3g0128691	58.696	138	50	3	4	138	14	147	9.66e-50	158
MsaG002097	MtrunA17_Chr3g0124861	50.676	148	57	3	6	138	20	166	7.77e-43	143
MsaG002097	MtrunA17_Chr4g0021731	59.756	82	30	2	7	88	10	88	4.85e-25	93.2
MsaG002097	MtrunA17_Chr4g0030271	35.075	134	75	2	8	141	89	210	2.36e-17	79.3
MsaG002097	MtrunA17_Chr3g0127941	29.932	147	88	2	8	151	56	190	7.86e-17	77.8
MsaG002097	MtrunA17_Chr7g0276361	30.921	152	93	2	10	161	16	155	5.82e-16	75.5
MsaG002097	MtrunA17_Chr8g0345901	30.882	136	82	1	8	143	36	159	5.37e-14	69.7
MsaG002097	MtrunA17_Chr1g0181811	28.788	132	82	1	10	141	87	206	2.78e-13	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002097	AT1G59940.1	59.406	202	59	5	4	186	29	226	7.48e-72	217
MsaG002097	AT1G59940.2	59.406	202	59	5	4	186	121	318	2.45e-70	216
MsaG002097	AT1G10470.3	70.199	151	40	3	6	155	18	164	2.81e-69	211
MsaG002097	AT1G10470.1	70.199	151	40	3	6	155	32	178	7.17e-69	211
MsaG002097	AT3G48100.1	64.744	156	53	1	8	163	25	178	1.81e-64	197
MsaG002097	AT5G62920.1	72.656	128	32	1	8	135	25	149	2.37e-61	189
MsaG002097	AT1G74890.1	71.318	129	34	1	7	135	17	142	3.10e-59	184
MsaG002097	AT1G19050.1	65.441	136	44	1	7	142	23	155	1.75e-58	182
MsaG002097	AT2G40670.1	59.259	135	55	0	4	138	25	159	9.92e-55	171
MsaG002097	AT3G57040.2	58.993	139	50	2	7	138	8	146	1.57e-53	171
MsaG002097	AT3G57040.1	58.993	139	50	2	7	138	8	146	1.57e-53	171
MsaG002097	AT3G56380.1	61.194	134	48	2	6	138	18	148	2.02e-53	168
MsaG002097	AT2G41310.1	59.441	143	52	2	1	138	3	144	3.72e-53	169
MsaG002097	AT2G40670.2	58.824	136	55	1	4	138	25	160	5.85e-53	167
MsaG002097	AT3G56380.2	60.741	135	48	3	6	138	18	149	1.34e-51	163
MsaG002097	AT1G10470.2	56.044	91	30	6	70	155	17	102	1.39e-23	92.8
MsaG002097	AT2G46790.3	31.343	134	80	2	8	141	37	158	2.76e-15	73.6
MsaG002097	AT5G24470.1	30.216	139	83	3	8	146	52	176	3.21e-15	73.6
MsaG002097	AT2G46790.1	31.343	134	80	2	8	141	37	158	3.81e-15	73.2
MsaG002097	AT5G60100.7	34.848	132	74	2	10	141	66	185	1.61e-14	71.2
MsaG002097	AT5G60100.6	34.848	132	74	2	10	141	66	185	1.61e-14	71.2
MsaG002097	AT5G60100.4	34.848	132	74	2	10	141	66	185	1.61e-14	71.2
MsaG002097	AT5G60100.2	34.848	132	74	2	10	141	66	185	2.62e-14	70.9
MsaG002097	AT5G60100.5	34.848	132	74	2	10	141	66	185	3.26e-14	70.5
MsaG002097	AT5G60100.3	34.848	132	74	2	10	141	66	185	3.26e-14	70.5
MsaG002097	AT5G60100.1	34.848	132	74	2	10	141	66	185	3.26e-14	70.5
MsaG002097	AT2G07440.1	60.000	50	20	0	89	138	9	58	1.66e-13	65.1
MsaG002097	AT5G02810.1	28.358	134	84	1	8	141	78	199	1.21e-12	66.2

Find 38 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGTGATTACATGCTTCTTC+TGG	0.290924	1:+32251842	None:intergenic
ATTGAAAATATTCAGATGTT+TGG	0.307010	1:-32251978	MsaT002097.1:intron
AACTCATAACCAGTCATTTC+TGG	0.328147	1:+32252809	None:intergenic
TTTGTTGCCTTAGCCTTTGA+AGG	0.366124	1:-32252861	MsaT002097.1:intron
CTCAAAATTTCTGCTTGTAA+AGG	0.386939	1:-32253267	MsaT002097.1:intron
TTCTGAAGAGGTTCATGTAT+TGG	0.402386	1:-32253334	MsaT002097.1:CDS
TACTGGAATTTCCTTAAAAG+TGG	0.438337	1:+32252652	None:intergenic
TTAGTTACTGCTGTGGATAG+TGG	0.438893	1:-32253040	MsaT002097.1:intron
TGATGAAGGTGATGATGAAA+TGG	0.459647	1:+32251805	None:intergenic
TTCACATCACATAACTTCAC+TGG	0.475573	1:+32251926	None:intergenic
ATAACAGTACCTGTCTATGC+GGG	0.487586	1:+32252601	None:intergenic
TCAGAAGACATAATCACTAC+TGG	0.489512	1:+32252635	None:intergenic
ATATTCTGCCCCGCATAGAC+AGG	0.510615	1:-32252610	MsaT002097.1:intron
ATATTCAGATGTTTGGAAGA+AGG	0.511460	1:-32251971	MsaT002097.1:intron
TATTGCATGCCAGAAATGAC+TGG	0.520879	1:-32252818	MsaT002097.1:CDS
TTGATCGGAAAGTTATTGAA+AGG	0.524082	1:-32253293	MsaT002097.1:CDS
TGACAACCCAAATAGTCATT+CGG	0.532453	1:+32251721	None:intergenic
ATAACAAGATCCACCTTCAA+AGG	0.539532	1:+32252848	None:intergenic
GTTGCCTTAGCCTTTGAAGG+TGG	0.539999	1:-32252858	MsaT002097.1:intron
TGAAGGGTTACATGACAACT+AGG	0.542782	1:-32251898	MsaT002097.1:CDS
TACATGACAACTAGGGAGGT+TGG	0.550651	1:-32251890	MsaT002097.1:CDS
TGCTTGAGTCTCCAATCAGA+CGG	0.583623	1:-32251760	MsaT002097.1:CDS
CATCACCTTCATCATCTCCA+TGG	0.584093	1:-32251796	MsaT002097.1:CDS
ACAACTAGGGAGGTTGGAGT+AGG	0.598300	1:-32251884	MsaT002097.1:CDS
ATGTTTGGAAGAAGGTGCAA+AGG	0.598645	1:-32251963	MsaT002097.1:CDS
AGAGAGAAGTCTGAATCTGA+TGG	0.604808	1:-32252980	MsaT002097.1:CDS
GTGAACTTTAGTTACTGCTG+TGG	0.606977	1:-32253047	MsaT002097.1:intron
GATGGAGACAAATTCTGAAG+AGG	0.610289	1:-32253346	MsaT002097.1:CDS
TTGATGATAGTCTCATTGAT+CGG	0.612021	1:-32253308	MsaT002097.1:CDS
AGCACACTAGATGCAGACCA+TGG	0.620424	1:+32251779	None:intergenic
GCAGACCATGGAGATGATGA+AGG	0.631715	1:+32251791	None:intergenic
ATGTGATGTGAAACGACTGA+AGG	0.663812	1:-32251915	MsaT002097.1:CDS
GAAGGGTTACATGACAACTA+GGG	0.665755	1:-32251897	MsaT002097.1:CDS
TGTGATGTGAAACGACTGAA+GGG	0.670059	1:-32251914	MsaT002097.1:CDS
TATAACAGTACCTGTCTATG+CGG	0.670864	1:+32252600	None:intergenic
GAAGGTGATGATGAAATGGA+AGG	0.675343	1:+32251809	None:intergenic
TAACAGTACCTGTCTATGCG+GGG	0.697429	1:+32252602	None:intergenic
GGGTTACATGACAACTAGGG+AGG	0.704136	1:-32251894	MsaT002097.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	32251736	32253367	32251736	ID=MsaG002097
Chr1	mRNA	32251736	32253367	32251736	ID=MsaT002097.1;Parent=MsaG002097
Chr1	exon	32251736	32251985	32251736	ID=MsaT002097.1.exon5;Parent=MsaT002097.1
Chr1	CDS	32251736	32251985	32251736	ID=cds.MsaT002097.1;Parent=MsaT002097.1
Chr1	exon	32252611	32252681	32252611	ID=MsaT002097.1.exon4;Parent=MsaT002097.1
Chr1	CDS	32252611	32252681	32252611	ID=cds.MsaT002097.1;Parent=MsaT002097.1
Chr1	exon	32252793	32252870	32252793	ID=MsaT002097.1.exon3;Parent=MsaT002097.1
Chr1	CDS	32252793	32252870	32252793	ID=cds.MsaT002097.1;Parent=MsaT002097.1
Chr1	exon	32252973	32253058	32252973	ID=MsaT002097.1.exon2;Parent=MsaT002097.1
Chr1	CDS	32252973	32253058	32252973	ID=cds.MsaT002097.1;Parent=MsaT002097.1
Chr1	exon	32253268	32253367	32253268	ID=MsaT002097.1.exon1;Parent=MsaT002097.1
Chr1	CDS	32253268	32253367	32253268	ID=cds.MsaT002097.1;Parent=MsaT002097.1

Gene Sequence

>MsaT002097.1

ATGGAGACAAATTCTGAAGAGGTTCATGTATTGGCTGTTGATGATAGTCTCATTGATCGGAAAGTTATTGAAAGGTTGCTCAAAATTTCTGCTTGTAAAGTTACTGCTGTGGATAGTGGAATCAGAGCACTGCAATTTTTGGGGTTGGATGAGCAGAGAGAGAAGTCTGAATCTGATGGTTCTATTCCTTTGAAGGTGGATCTTGTTATTACAGATTATTGCATGCCAGAAATGACTGGTTATGAGTTGCTCAAAAAAATTAAGGAATCGTCCACTTTTAAGGAAATTCCAGTAGTGATTATGTCTTCTGAAAATATTCTGCCCCGCATAGACAGATGTTTGGAAGAAGGTGCAAAGGATTTTATAATAAAGCCAGTGAAGTTATGTGATGTGAAACGACTGAAGGGTTACATGACAACTAGGGAGGTTGGAGTAGGAATCAACAATAAACAGAAGCCAGAAGAAGCATGTAATCACTCATCATCACAACCTTCCATTTCATCATCACCTTCATCATCTCCATGGTCTGCATCTAGTGTGCTTGAGTCTCCAATCAGACGGTTTAAAATGACTGACACCGAATGA

Protein sequence

>MsaT002097.1

METNSEEVHVLAVDDSLIDRKVIERLLKISACKVTAVDSGIRALQFLGLDEQREKSESDGSIPLKVDLVITDYCMPEMTGYELLKKIKESSTFKEIPVVIMSSENILPRIDRCLEEGAKDFIIKPVKLCDVKRLKGYMTTREVGVGINNKQKPEEACNHSSSQPSISSSPSSSPWSASSVLESPIRRFKMTDTE*