AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002142


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002142 MtrunA17_Chr1g0171291 87.879 198 8 2 1 198 1 182 6.07e-120 346
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002142 AT1G42990.1 56.250 64 27 1 136 198 155 218 6.24e-14 71.6

Find 86 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTCCCTGCTATGGTTCC+TGG 0.181734 1:+33016860 MsaT002142.1:CDS
CACCTTTGGACCAAAATAAA+AGG 0.232730 1:-33016570 None:intergenic
GAGAGATTCGAAATCGATTT+GGG 0.252626 1:-33014814 None:intergenic
CTAAGAAGGGTGGCTCTAAA+AGG 0.288472 1:+33016966 MsaT002142.1:CDS
AATACCTACTCTTCGTTTCA+AGG 0.332279 1:-33015455 None:intergenic
AGAGAGATTCGAAATCGATT+TGG 0.334822 1:-33014815 None:intergenic
TGTTGCAGAATCCCTGCTGC+TGG 0.361805 1:+33016839 MsaT002142.1:intron
GGAGTCTGCTGTGCTCTTGT+TGG 0.367032 1:+33015593 MsaT002142.1:CDS
GGAACCTCTTGAGGAGTTAC+TGG 0.369486 1:-33015080 None:intergenic
TGATCATCCGATTGAGGGAT+TGG 0.372571 1:-33015023 None:intergenic
TGAAGGGGAATGCAGAAGAT+TGG 0.381118 1:+33015479 MsaT002142.1:CDS
GGAACCTCTTGAGGAGTTAC+TGG 0.381189 1:-33015125 None:intergenic
TCAGAGATATTATTACTGTT+TGG 0.385576 1:-33016990 None:intergenic
TACATAAAGCTCAATAACTA+TGG 0.394517 1:-33016356 None:intergenic
TTGCAATCGCGTGGTGGCTT+TGG 0.414223 1:+33015552 MsaT002142.1:CDS
TTTCAGGCAGATGAGGAATA+GGG 0.426727 1:+33015386 MsaT002142.1:intron
AGCAAAAGGATGTTCACCTT+TGG 0.429049 1:-33016584 None:intergenic
GGTTCCCTGCTATGGTTCCT+GGG 0.429306 1:+33016861 MsaT002142.1:CDS
TGCTGCTGGGTTCCCTGCTA+TGG 0.441437 1:+33016853 MsaT002142.1:CDS
GAGTCTGCTGTGCTCTTGTT+GGG 0.447976 1:+33015594 MsaT002142.1:CDS
TGGTGCCCAGGAACCATAGC+AGG 0.462764 1:-33016866 None:intergenic
TCATTCTGACAAAGACCAAC+AGG 0.468319 1:-33016530 None:intergenic
CAAATCTGGATTACAGCAAA+AGG 0.469492 1:-33016598 None:intergenic
AACCTTGATCATCCGATTGA+GGG 0.476612 1:-33015028 None:intergenic
CCTCGACCCAATCCCTCAAT+CGG 0.479515 1:+33015016 MsaT002142.1:CDS
AGGTTCCTTCTGATGAAGGC+AGG 0.489060 1:+33015096 MsaT002142.1:CDS
GGGAATTGCGTCGTCATCAT+CGG 0.491346 1:+33014959 MsaT002142.1:CDS
GATCATCCGATTGAGGGATT+GGG 0.498027 1:-33015022 None:intergenic
AGCAGACTCCTGCATGGTCA+TGG 0.499805 1:-33015580 None:intergenic
CAAGAGGTTCCTTCTGATGA+AGG 0.500088 1:+33015092 MsaT002142.1:CDS
ATAGCAGGGAACCCAGCAGC+AGG 0.506596 1:-33016851 None:intergenic
ATGCTGAGAACCATGCTTTG+CGG 0.510527 1:+33015523 MsaT002142.1:CDS
AAACCTTGATCATCCGATTG+AGG 0.512112 1:-33015029 None:intergenic
CTGTTGTTAGCCACAGTGGA+AGG 0.514860 1:-33016908 None:intergenic
TGGAACACAAAGGTCTAAGA+AGG 0.519321 1:+33016952 MsaT002142.1:CDS
CCGATTGAGGGATTGGGTCG+AGG 0.520843 1:-33015016 None:intergenic
GGACCAAAATAAAAGGCTAC+AGG 0.521330 1:-33016563 None:intergenic
TGGTGCTCCCATGACCATGC+AGG 0.523079 1:+33015572 MsaT002142.1:CDS
GTTGCAGAATCCCTGCTGCT+GGG 0.537879 1:+33016840 MsaT002142.1:intron
TCTTCTCATGTCTGATGCCG+AGG 0.542387 1:+33014938 MsaT002142.1:CDS
AGGAGTTACTGGATCAACTC+TGG 0.544068 1:-33015069 None:intergenic
TGAGTTGGCACATGGTGCCC+AGG 0.544147 1:-33016878 None:intergenic
GTTGTTAGCCACAGTGGAAG+GGG 0.545341 1:-33016906 None:intergenic
ATCCCTCAATCGGATGATCA+AGG 0.545906 1:+33015026 MsaT002142.1:CDS
TTCGAGAAACTTATGATAGT+CGG 0.546362 1:-33014985 None:intergenic
TCAACTCTGGCTTCATCAGA+AGG 0.547608 1:-33015056 None:intergenic
CTTTGTTTGCAATCGCGTGG+TGG 0.550397 1:+33015546 MsaT002142.1:CDS
TCATCAGAAGGAACCTCTTG+AGG 0.553244 1:-33015089 None:intergenic
TAAGAAGGGTGGCTCTAAAA+GGG 0.555483 1:+33016967 MsaT002142.1:CDS
GCAGACTCCTGCATGGTCAT+GGG 0.561161 1:-33015579 None:intergenic
TCAACCCTGCCTTCATCAGA+AGG 0.569263 1:-33015101 None:intergenic
ATAATATTTCACCTTCTCAC+CGG 0.569829 1:+33016326 MsaT002142.1:CDS
GCAAACAAAGCCGCAAAGCA+TGG 0.573484 1:-33015533 None:intergenic
TGATGACGACGCAATTCCCT+CGG 0.574197 1:-33014955 None:intergenic
TTGGGGTTCTAAATCGTCCA+TGG 0.576660 1:-33014796 None:intergenic
TCAATCTCTGAGAGAGGCGA+GGG 0.577892 1:-33014912 None:intergenic
TGTTGTTAGCCACAGTGGAA+GGG 0.582265 1:-33016907 None:intergenic
CTCAATCTCTGAGAGAGGCG+AGG 0.584965 1:-33014913 None:intergenic
GGTTCAGGAGGAACCTCTTG+AGG 0.589392 1:-33015134 None:intergenic
CAATAACTATGGTGATAGAC+CGG 0.591350 1:-33016345 None:intergenic
GTTACCTCCAAGAGAAAACA+TGG 0.596124 1:+33016932 MsaT002142.1:CDS
GGTTCCTTCTGATGAAGGCA+GGG 0.598695 1:+33015097 MsaT002142.1:CDS
TGATCCAGTAACTCCTCAAG+AGG 0.602779 1:+33015076 MsaT002142.1:CDS
ACAATCACAAAACCACCTGT+TGG 0.603331 1:+33016515 MsaT002142.1:CDS
GTGGAAGGGGTAGACTGAGT+TGG 0.606292 1:-33016893 None:intergenic
CGGCTTTGTTTGCAATCGCG+TGG 0.608761 1:+33015543 MsaT002142.1:CDS
GTGTCTGCGACGGAAACAGA+AGG 0.621465 1:-33014888 None:intergenic
CTTCTCATGTCTGATGCCGA+GGG 0.622387 1:+33014939 MsaT002142.1:CDS
GGGTAGACTGAGTTGGCACA+TGG 0.627667 1:-33016886 None:intergenic
AACACTGTTGTTAGCCACAG+TGG 0.628137 1:-33016912 None:intergenic
AGGCGAGGGAGTGTCTGCGA+CGG 0.634385 1:-33014898 None:intergenic
CCAAAATAAAAGGCTACAGG+AGG 0.635451 1:-33016560 None:intergenic
TGATCCAGTAACTCCTCAAG+AGG 0.636811 1:+33015121 MsaT002142.1:CDS
AGATTCTCAATCTCTGAGAG+AGG 0.640144 1:-33014918 None:intergenic
ATGGTGATAGACCGGTGAGA+AGG 0.641259 1:-33016337 None:intergenic
CTGCTGTGAAATCAAGAGAG+AGG 0.642211 1:+33015412 MsaT002142.1:CDS
AGAGATTCGAAATCGATTTG+GGG 0.646761 1:-33014813 None:intergenic
AAAACCTTGAAACGAAGAGT+AGG 0.647793 1:+33015451 MsaT002142.1:CDS
TAGCAGGGAACCCAGCAGCA+GGG 0.658619 1:-33016850 None:intergenic
GGAACACAAAGGTCTAAGAA+GGG 0.664648 1:+33016953 MsaT002142.1:CDS
ACACAAAGGTCTAAGAAGGG+TGG 0.678689 1:+33016956 MsaT002142.1:CDS
TTCTGACAAAGACCAACAGG+TGG 0.685708 1:-33016527 None:intergenic
GAGCACAGCAGACTCCTGCA+TGG 0.688063 1:-33015586 None:intergenic
GGTGCCCAGGAACCATAGCA+GGG 0.695738 1:-33016865 None:intergenic
AGAGAAAACATGGAACACAA+AGG 0.721160 1:+33016942 MsaT002142.1:CDS
TCAGTCTACCCCTTCCACTG+TGG 0.742334 1:+33016898 MsaT002142.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 33014798 33017000 33014798 ID=MsaG002142
Chr1 mRNA 33014798 33017000 33014798 ID=MsaT002142.1;Parent=MsaG002142
Chr1 exon 33014798 33015177 33014798 ID=MsaT002142.1.exon1;Parent=MsaT002142.1
Chr1 CDS 33014798 33015177 33014798 ID=cds.MsaT002142.1;Parent=MsaT002142.1
Chr1 exon 33015392 33015615 33015392 ID=MsaT002142.1.exon2;Parent=MsaT002142.1
Chr1 CDS 33015392 33015615 33015392 ID=cds.MsaT002142.1;Parent=MsaT002142.1
Chr1 exon 33016293 33016375 33016293 ID=MsaT002142.1.exon3;Parent=MsaT002142.1
Chr1 CDS 33016293 33016375 33016293 ID=cds.MsaT002142.1;Parent=MsaT002142.1
Chr1 exon 33016464 33016606 33016464 ID=MsaT002142.1.exon4;Parent=MsaT002142.1
Chr1 CDS 33016464 33016606 33016464 ID=cds.MsaT002142.1;Parent=MsaT002142.1
Chr1 exon 33016847 33017000 33016847 ID=MsaT002142.1.exon5;Parent=MsaT002142.1
Chr1 CDS 33016847 33017000 33016847 ID=cds.MsaT002142.1;Parent=MsaT002142.1
Gene Sequence

>MsaT002142.1

ATGGACGATTTAGAACCCCAAATCGATTTCGAATCTCTCTTCGCAGAATACGAAGTCGCCGATTTTTTGCAGGAGGATGAGGATATCAATCCTTCTGTTTCCGTCGCAGACACTCCCTCGCCTCTCTCAGAGATTGAGAATCTTCTCATGTCTGATGCCGAGGGAATTGCGTCGTCATCATCGGATTCCGACTATCATAAGTTTCTCGAAGATATTCTCCTCGACCCAATCCCTCAATCGGATGATCAAGGTTTCGTTCCTTCTGATGAAGCCAGAGTTGATCCAGTAACTCCTCAAGAGGTTCCTTCTGATGAAGGCAGGGTTGATCCAGTAACTCCTCAAGAGGTTCCTCCTGAACCCGTTTCAAAAAAGCAAATTAGGCAGATGAGGAATAGGGATGCTGCTGTGAAATCAAGAGAGAGGAAGAAGATGTATGTGAAAAACCTTGAAACGAAGAGTAGGTATTTTGAAGGGGAATGCAGAAGATTGGAGCATTTGCTTCAATGCTGCTATGCTGAGAACCATGCTTTGCGGCTTTGTTTGCAATCGCGTGGTGGCTTTGGTGCTCCCATGACCATGCAGGAGTCTGCTGTGCTCTTGTTGGTGTTCAGGCATCTCATTCTGTCTCATTCAAGCTATAATATTTCACCTTCTCACCGGTCTATCACCATAGTTATTGAGCTTTATGTTTTTGCATCTAAATGGAAAAAATTTGTGTGGGTTGTATGGATAATAATAACAATCACAAAACCACCTGTTGGTCTTTGTCAGAATGAAAGTCTACCTCCTGTAGCCTTTTATTTTGGTCCAAAGGTGAACATCCTTTTGCTAATCCCTGCTGCTGGGTTCCCTGCTATGGTTCCTGGGCACCATGTGCCAACTCAGTCTACCCCTTCCACTGTGGCTAACAACAGTGTTACCTCCAAGAGAAAACATGGAACACAAAGGTCTAAGAAGGGTGGCTCTAAAAGGGCCAAACAGTAA

Protein sequence

>MsaT002142.1

MDDLEPQIDFESLFAEYEVADFLQEDEDINPSVSVADTPSPLSEIENLLMSDAEGIASSSSDSDYHKFLEDILLDPIPQSDDQGFVPSDEARVDPVTPQEVPSDEGRVDPVTPQEVPPEPVSKKQIRQMRNRDAAVKSRERKKMYVKNLETKSRYFEGECRRLEHLLQCCYAENHALRLCLQSRGGFGAPMTMQESAVLLLVFRHLILSHSSYNISPSHRSITIVIELYVFASKWKKFVWVVWIIITITKPPVGLCQNESLPPVAFYFGPKVNILLLIPAAGFPAMVPGHHVPTQSTPSTVANNSVTSKRKHGTQRSKKGGSKRAKQ*