AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002183

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002183 MtrunA17_Chr1g0163181 66.554 296 91 6 1 292 1 292 1.84e-128 372
MsaG002183 MtrunA17_Chr1g0163181 82.051 78 14 0 218 295 355 432 1.23e-38 140
MsaG002183 MtrunA17_Chr1g0163201 59.364 283 89 6 20 292 18 284 7.20e-96 288
MsaG002183 MtrunA17_Chr1g0163201 72.222 108 25 1 188 295 293 395 6.93e-46 159
MsaG002183 MtrunA17_Chr1g0163251 49.667 300 122 4 1 292 1 279 2.39e-93 281
MsaG002183 MtrunA17_Chr1g0163191 76.786 168 32 2 1 168 1 161 6.18e-81 241
MsaG002183 MtrunA17_Chr1g0163241 62.857 175 42 4 4 177 10 162 3.77e-65 201
MsaG002183 MtrunA17_Chr1g0163231 37.987 308 145 7 1 292 1 278 1.61e-62 199
MsaG002183 MtrunA17_Chr1g0163261 37.013 308 152 5 1 292 1 282 1.37e-61 197
MsaG002183 MtrunA17_Chr1g0171611 38.033 305 137 7 1 292 1 266 8.07e-59 190
MsaG002183 MtrunA17_Chr1g0163131 39.590 293 154 9 14 294 27 308 1.97e-54 179
MsaG002183 MtrunA17_Chr1g0163111 76.543 81 19 0 216 296 152 232 2.67e-37 132
MsaG002183 MtrunA17_Chr1g0163111 41.964 112 59 3 183 291 1 109 7.34e-17 78.2
MsaG002183 MtrunA17_Chr1g0163151 77.632 76 17 0 218 293 147 222 1.08e-35 128
MsaG002183 MtrunA17_Chr1g0163151 39.286 112 65 2 183 291 1 112 5.39e-19 83.6
MsaG002183 MtrunA17_Chr3g0131891 32.759 290 167 9 14 291 28 301 5.38e-34 126
MsaG002183 MtrunA17_Chr7g0233521 30.496 282 163 9 21 291 2 261 5.47e-31 117
MsaG002183 MtrunA17_Chr3g0103181 28.522 291 176 9 9 291 12 278 5.70e-31 117
MsaG002183 MtrunA17_Chr7g0233541 29.352 293 169 9 21 291 2 278 3.56e-30 115
MsaG002183 MtrunA17_Chr1g0163101 45.763 118 61 2 14 131 26 140 1.95e-29 110
MsaG002183 MtrunA17_Chr3g0103171 26.829 287 168 8 20 291 5 264 3.17e-29 112
MsaG002183 MtrunA17_Chr7g0233561 30.592 304 181 10 8 291 17 310 2.16e-28 111
MsaG002183 MtrunA17_Chr8g0390841 27.211 294 186 8 14 291 48 329 3.18e-28 113
MsaG002183 MtrunA17_Chr3g0103191 29.924 264 148 9 9 259 12 251 1.80e-27 107
MsaG002183 MtrunA17_Chr1g0208771 27.148 291 179 8 20 291 12 288 1.98e-27 110
MsaG002183 MtrunA17_Chr1g0162161 34.395 157 86 3 21 177 4 143 1.77e-25 99.4
MsaG002183 MtrunA17_Chr6g0476181 37.037 162 72 4 21 179 2 136 5.76e-25 98.2
MsaG002183 MtrunA17_Chr7g0233531 28.169 284 167 8 21 291 2 261 1.15e-24 100
MsaG002183 MtrunA17_Chr1g0163121 44.954 109 57 2 19 127 9 114 1.33e-24 96.7
MsaG002183 MtrunA17_Chr1g0163071 25.000 292 190 7 21 291 2 285 1.99e-22 94.7
MsaG002183 MtrunA17_Chr1g0163081 40.187 107 61 2 21 127 6 109 3.30e-22 90.5
MsaG002183 MtrunA17_Chr7g0233571 31.780 236 102 7 9 213 10 217 3.70e-22 92.4
MsaG002183 MtrunA17_Chr3g0103151 31.818 154 93 4 9 158 12 157 1.24e-19 84.0
MsaG002183 MtrunA17_Chr1g0163141 34.356 163 90 4 17 179 7 152 2.25e-19 84.0
MsaG002183 MtrunA17_Chr1g0163221 26.207 290 157 10 14 279 20 276 2.44e-19 87.0
MsaG002183 MtrunA17_Chr4g0067431 27.241 290 185 10 9 279 15 297 2.45e-19 86.7
MsaG002183 MtrunA17_Chr5g0419201 30.201 149 98 2 14 160 25 169 4.23e-19 83.2
MsaG002183 MtrunA17_Chr1g0163161 39.394 99 57 2 33 131 1 96 6.73e-18 78.6
MsaG002183 MtrunA17_Chr6g0467351 35.514 107 66 2 13 116 23 129 7.91e-16 75.1
MsaG002183 MtrunA17_Chr3g0103201 26.360 239 141 8 64 291 5 219 1.84e-14 71.2
MsaG002183 MtrunA17_Chr6g0476171 54.098 61 28 0 232 292 185 245 1.87e-13 68.9
MsaG002183 MtrunA17_Chr4g0033961 35.644 101 59 2 7 101 4 104 8.38e-13 68.2
MsaG002183 MtrunA17_Chr1g0154131 24.415 299 188 9 16 295 193 472 2.30e-12 67.0
MsaG002183 MtrunA17_Chr1g0162441 32.432 111 68 3 7 110 5 115 1.04e-11 65.1
MsaG002183 MtrunA17_Chr1g0163061 35.165 91 56 2 14 101 24 114 3.61e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002183 AT3G18990.1 26.036 338 200 12 7 296 4 339 2.60e-22 95.5
MsaG002183 AT3G18990.2 26.205 332 195 12 7 290 4 333 4.31e-22 95.1

Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACACCGTTTGGAAATTTA+AGG 0.151014 1:+33579283 None:intergenic
TTTGCAAAGCCTTTCAAATA+TGG 0.200041 1:-33579198 MsaT002183.1:CDS
TATTTCTCCACATATTTCTT+AGG 0.222742 1:+33579333 None:intergenic
AAATATTGTGGAATTGAAAA+TGG 0.233869 1:-33577546 MsaT002183.1:CDS
GTGTTTAATGTTTCAATTGT+AGG 0.249847 1:-33577377 MsaT002183.1:CDS
AGAAATATGTGGAGAAATAT+TGG 0.283749 1:-33579329 MsaT002183.1:CDS
TGGAGAGTAATGGTGATATT+TGG 0.287546 1:-33579242 MsaT002183.1:CDS
TTTGGAAATTTAAGGAATAT+AGG 0.302976 1:+33579291 None:intergenic
TTCATCAACAGATTTGATAT+TGG 0.305884 1:+33579092 None:intergenic
TTACACCAATGGCTATTTCT+TGG 0.306445 1:-33578428 MsaT002183.1:intron
CATTAACAGCTTCAGATGTT+AGG 0.318603 1:+33578485 None:intergenic
TCCTCTCATCAATCAACTTA+AGG 0.325864 1:+33577405 None:intergenic
TTGGAAATTTAAGGAATATA+GGG 0.346499 1:+33579292 None:intergenic
TTAACTTTCAAATTCATTAG+TGG 0.350653 1:-33579168 MsaT002183.1:CDS
TGGCAAAAGAAAAGCGAGTA+TGG 0.368217 1:-33578920 MsaT002183.1:CDS
AAATGCCAAACCAAGCTAAA+AGG 0.394610 1:-33578951 MsaT002183.1:CDS
ATAATTTATTTCTAAAGTAT+TGG 0.398912 1:+33579116 None:intergenic
AATATTGTGGAATTGAAAAT+GGG 0.408122 1:-33577545 MsaT002183.1:CDS
AATTATTATCAGAGTCTTTG+TGG 0.412362 1:-33577497 MsaT002183.1:CDS
AAAGAAGGCAAAGTGGAGAT+TGG 0.415515 1:-33577440 MsaT002183.1:CDS
AAGATTATCCTATGAAAATC+TGG 0.419859 1:+33579504 None:intergenic
TGGAGAAAATTTATGAAAGA+AGG 0.431214 1:-33577455 MsaT002183.1:CDS
CAAGCTAAAAGGACAAGAAA+TGG 0.443867 1:-33578940 MsaT002183.1:CDS
CAAAGTAGCAATGGTGAAGA+AGG 0.457353 1:-33578518 MsaT002183.1:CDS
TGTCATTATAACTTCAAAAG+AGG 0.475259 1:+33578456 None:intergenic
CCGAAGGAGTTTATGAAAAG+AGG 0.504433 1:-33577578 MsaT002183.1:CDS
GTCATTATAACTTCAAAAGA+GGG 0.507540 1:+33578457 None:intergenic
AATATCAAATCTGTTGATGA+AGG 0.508408 1:-33579090 MsaT002183.1:CDS
ACCACCCAACCAAAATTCTC+AGG 0.510316 1:-33578889 MsaT002183.1:intron
AAGAGATATCCATATTTGAA+AGG 0.520085 1:+33579189 None:intergenic
AGGATGTCATGAAAATATTG+TGG 0.531604 1:-33577558 MsaT002183.1:CDS
ATATTCCTTAAATTTCCAAA+CGG 0.536219 1:-33579288 MsaT002183.1:CDS
TGTGGCTGCAGATTCAGTAA+AGG 0.542328 1:-33577479 MsaT002183.1:CDS
AGCTGAAGACACAGAAGAAG+AGG 0.546039 1:-33579067 MsaT002183.1:CDS
GTATTTGCAGTGTGTACCGA+AGG 0.559027 1:-33577594 MsaT002183.1:intron
GCTGCAGATTCAGTAAAGGT+TGG 0.573667 1:-33577475 MsaT002183.1:CDS
TGATGACAGTTCAGATGAGG+TGG 0.583984 1:-33578974 MsaT002183.1:CDS
ACTTACCAAGAAATAGCCAT+TGG 0.587587 1:+33578423 None:intergenic
GAGGGTTCCTAAGAAATATG+TGG 0.594786 1:-33579340 MsaT002183.1:CDS
ATTTATGAAAGAAGGCAAAG+TGG 0.595070 1:-33577447 MsaT002183.1:CDS
TCCTTAAGTTGATTGATGAG+AGG 0.601248 1:-33577406 MsaT002183.1:CDS
AGGTAGTAACAAAGTAGCAA+TGG 0.603454 1:-33578527 MsaT002183.1:CDS
ATGACACAATCTTACACCAA+TGG 0.609025 1:-33578439 MsaT002183.1:CDS
TGAAAATGGGGAGAAAGTCA+TGG 0.612079 1:-33577532 MsaT002183.1:CDS
TATGTGGAGAAATATTGGAA+AGG 0.617787 1:-33579324 MsaT002183.1:CDS
ATATTGTGGAATTGAAAATG+GGG 0.634099 1:-33577544 MsaT002183.1:CDS
GCTGAAGACACAGAAGAAGA+GGG 0.643743 1:-33579066 MsaT002183.1:CDS
AAGTGATGACAGTTCAGATG+AGG 0.649994 1:-33578977 MsaT002183.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 33577364 33579544 33577364 ID=MsaG002183
Chr1 mRNA 33577364 33579544 33577364 ID=MsaT002183.1;Parent=MsaG002183
Chr1 exon 33577364 33577606 33577364 ID=MsaT002183.1.exon4;Parent=MsaT002183.1
Chr1 CDS 33577364 33577606 33577364 ID=cds.MsaT002183.1;Parent=MsaT002183.1
Chr1 exon 33578429 33578547 33578429 ID=MsaT002183.1.exon3;Parent=MsaT002183.1
Chr1 CDS 33578429 33578547 33578429 ID=cds.MsaT002183.1;Parent=MsaT002183.1
Chr1 exon 33578890 33579361 33578890 ID=MsaT002183.1.exon2;Parent=MsaT002183.1
Chr1 CDS 33578890 33579361 33578890 ID=cds.MsaT002183.1;Parent=MsaT002183.1
Chr1 exon 33579488 33579544 33579488 ID=MsaT002183.1.exon1;Parent=MsaT002183.1
Chr1 CDS 33579488 33579544 33579488 ID=cds.MsaT002183.1;Parent=MsaT002183.1
Gene Sequence

>MsaT002183.1

ATGTTAGTTCAACATGTTCCAGATTTTCATAGGATAATCTTGCAAGATAAGAAACTTAGGGTTCCTAAGAAATATGTGGAGAAATATTGGAAAGGAATATCAAACCCTATATTCCTTAAATTTCCAAACGGTGTTGAACAAAAAATATTTTGGGTGGAGAGTAATGGTGATATTTGGTTTCAAAAAAATTGGGAAAGTTTTGCAAAGCCTTTCAAATATGGATATCTCTTAACTTTCAAATTCATTAGTGGATCATATTTTAAGGTGAAGATATTTGGTGCCAATACTTTAGAAATAAATTATGCCAATATCAAATCTGTTGATGAAGGAGCTGAAGACACAGAAGAAGAGGGTAAAGAAAGTGATGAATCAAGTGATAATGAAGCCACAGAAGAAAATCATGAAGAGAGTGATGAATCAAGTGATGACAGTTCAGATGAGGTGGAAATGCCAAACCAAGCTAAAAGGACAAGAAATGGCAAAAGAAAAGCGAGTATGGATTTTGACACCACCCAACCAAAATTCTCAGGTAGTAACAAAGTAGCAATGGTGAAGAAGGCTAGAAAATACCTAACATCTGAAGCTGTTAATGAGAATCCCTCTTTTGAAGTTATAATGACACAATCTTACACCAATGGCTATTTCTTGTGTGTACCGAAGGAGTTTATGAAAAGAGGATGTCATGAAAATATTGTGGAATTGAAAATGGGGAGAAAGTCATGGTTCGTAAAAGTCAATTATTATCAGAGTCTTTGTGGCTGCAGATTCAGTAAAGGTTGGAGAAAATTTATGAAAGAAGGCAAAGTGGAGATTGGAGATACTTGTCTCCTTAAGTTGATTGATGAGAGGAAATTTGTGTTTAATGTTTCAATTGTAGGAAAGAACCAATAA
Protein sequence

>MsaT002183.1

MLVQHVPDFHRIILQDKKLRVPKKYVEKYWKGISNPIFLKFPNGVEQKIFWVESNGDIWFQKNWESFAKPFKYGYLLTFKFISGSYFKVKIFGANTLEINYANIKSVDEGAEDTEEEGKESDESSDNEATEENHEESDESSDDSSDEVEMPNQAKRTRNGKRKASMDFDTTQPKFSGSNKVAMVKKARKYLTSEAVNENPSFEVIMTQSYTNGYFLCVPKEFMKRGCHENIVELKMGRKSWFVKVNYYQSLCGCRFSKGWRKFMKEGKVEIGDTCLLKLIDERKFVFNVSIVGKNQ*