AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002184


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002184 MtrunA17_Chr1g0163181 61.620 284 90 5 26 292 11 292 1.88e-108 322
MsaG002184 MtrunA17_Chr1g0163181 81.250 80 15 0 217 296 354 433 4.60e-39 142
MsaG002184 MtrunA17_Chr1g0163201 61.538 273 94 5 35 296 18 290 2.07e-102 305
MsaG002184 MtrunA17_Chr1g0163201 91.250 80 7 0 217 296 317 396 5.01e-45 157
MsaG002184 MtrunA17_Chr1g0163251 48.639 294 111 7 26 296 11 287 7.86e-79 244
MsaG002184 MtrunA17_Chr1g0163191 81.333 150 27 1 26 175 11 159 8.24e-71 216
MsaG002184 MtrunA17_Chr1g0163231 41.007 278 133 4 29 292 18 278 1.16e-63 202
MsaG002184 MtrunA17_Chr1g0171611 41.697 271 129 6 29 292 18 266 1.64e-59 191
MsaG002184 MtrunA17_Chr1g0163241 62.733 161 43 4 13 172 4 148 2.50e-57 182
MsaG002184 MtrunA17_Chr1g0163261 38.043 276 148 5 29 292 18 282 6.44e-57 185
MsaG002184 MtrunA17_Chr1g0163131 38.966 290 145 9 29 294 27 308 3.85e-53 176
MsaG002184 MtrunA17_Chr1g0163111 79.747 79 16 0 216 294 152 230 5.14e-39 137
MsaG002184 MtrunA17_Chr1g0163111 40.870 115 60 3 181 292 1 110 2.37e-17 79.3
MsaG002184 MtrunA17_Chr1g0163151 77.922 77 17 0 217 293 146 222 6.98e-38 133
MsaG002184 MtrunA17_Chr1g0163151 39.130 115 65 3 181 292 1 113 5.93e-18 80.9
MsaG002184 MtrunA17_Chr3g0103181 29.617 287 159 11 26 291 14 278 2.42e-31 118
MsaG002184 MtrunA17_Chr1g0163101 43.333 150 66 4 29 174 26 160 3.89e-31 115
MsaG002184 MtrunA17_Chr3g0131891 33.688 282 160 9 29 291 28 301 5.05e-31 118
MsaG002184 MtrunA17_Chr3g0103171 30.108 279 154 9 35 291 5 264 1.26e-30 115
MsaG002184 MtrunA17_Chr7g0233521 30.741 270 163 8 36 291 2 261 2.48e-30 115
MsaG002184 MtrunA17_Chr7g0233561 29.592 294 156 9 34 291 32 310 2.58e-28 111
MsaG002184 MtrunA17_Chr1g0208771 26.855 283 175 8 35 291 12 288 3.89e-28 112
MsaG002184 MtrunA17_Chr7g0233541 29.514 288 160 10 36 291 2 278 5.63e-27 107
MsaG002184 MtrunA17_Chr8g0390841 26.871 294 172 8 29 291 48 329 6.26e-27 109
MsaG002184 MtrunA17_Chr1g0163121 46.789 109 55 2 34 142 9 114 6.69e-26 100
MsaG002184 MtrunA17_Chr1g0162161 38.849 139 76 3 36 174 4 133 9.25e-26 100
MsaG002184 MtrunA17_Chr3g0103191 29.478 268 148 11 16 259 1 251 6.77e-25 100
MsaG002184 MtrunA17_Chr6g0476181 42.188 128 62 4 36 162 2 118 1.12e-23 94.7
MsaG002184 MtrunA17_Chr1g0163081 38.525 122 61 2 36 157 6 113 2.94e-23 93.2
MsaG002184 MtrunA17_Chr7g0233531 29.151 271 166 8 36 291 2 261 2.09e-21 91.7
MsaG002184 MtrunA17_Chr1g0163141 30.964 197 118 4 32 227 7 186 2.84e-20 86.3
MsaG002184 MtrunA17_Chr4g0067431 28.621 290 162 11 26 279 17 297 1.82e-19 87.0
MsaG002184 MtrunA17_Chr5g0419201 31.138 167 107 3 23 186 19 180 2.13e-19 84.0
MsaG002184 MtrunA17_Chr7g0233571 29.703 202 122 6 29 213 19 217 1.32e-18 82.8
MsaG002184 MtrunA17_Chr1g0163221 28.788 264 168 9 29 279 20 276 1.32e-18 84.7
MsaG002184 MtrunA17_Chr1g0163071 24.497 298 169 8 36 291 2 285 3.02e-18 83.2
MsaG002184 MtrunA17_Chr1g0163161 42.105 95 53 2 48 142 1 93 1.37e-17 77.4
MsaG002184 MtrunA17_Chr3g0103151 33.884 121 69 3 26 142 14 127 3.07e-17 77.4
MsaG002184 MtrunA17_Chr6g0476171 55.738 61 27 0 232 292 185 245 4.33e-16 76.3
MsaG002184 MtrunA17_Chr3g0103201 27.586 232 132 8 79 291 5 219 2.85e-15 73.6
MsaG002184 MtrunA17_Chr6g0467351 31.897 116 76 2 23 135 18 133 3.01e-14 70.9
MsaG002184 MtrunA17_Chr3g0077461 22.794 272 152 6 17 283 240 458 2.50e-12 67.0
MsaG002184 MtrunA17_Chr4g0033961 34.884 129 71 4 29 145 15 142 1.01e-11 65.1
MsaG002184 MtrunA17_Chr3g0077411 24.138 290 166 11 17 291 265 515 1.58e-11 64.7
MsaG002184 MtrunA17_Chr1g0163061 31.731 104 68 2 16 116 11 114 6.56e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002184 AT3G18990.2 22.866 328 178 8 29 290 15 333 4.50e-18 84.0
MsaG002184 AT3G18990.1 22.866 328 178 8 29 290 15 333 5.71e-18 83.2

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACACCGTTTGGAAATTTA+AGG 0.151014 1:+33585184 None:intergenic
TTTGCAAAGCCTTTCAAATA+TGG 0.200041 1:-33585099 MsaT002184.1:CDS
CAAGCTCAAAGGACAATAAA+AGG 0.217300 1:-33584859 MsaT002184.1:CDS
AAATATTGTGGAGTTGAAAA+TGG 0.256652 1:-33583688 MsaT002184.1:CDS
TGGAGAGAAATGGTGATATT+TGG 0.267670 1:-33585143 MsaT002184.1:CDS
TATTTCTCCACATATATCTT+AGG 0.281171 1:+33585234 None:intergenic
TTTGGAAATTTAAGGAATAT+AGG 0.302976 1:+33585192 None:intergenic
TTACACCAATGGCTATTTCT+TGG 0.306445 1:-33584526 MsaT002184.1:intron
GTGTTTGATGTTTCAATTGT+AGG 0.309600 1:-33583519 MsaT002184.1:CDS
CTCTTAACTTTCAAATTCAT+TGG 0.314459 1:-33585072 MsaT002184.1:CDS
AGATATATGTGGAGAAATAT+TGG 0.326941 1:-33585230 MsaT002184.1:CDS
TTGGAAATTTAAGGAATATA+GGG 0.346499 1:+33585193 None:intergenic
ATAGTTTATTTCTAAAGTAT+TGG 0.406626 1:+33585017 None:intergenic
ACAGCTTCAGATTGTGCCAT+TGG 0.421914 1:+33584588 None:intergenic
TACCGAATTGCCACTGAGCT+TGG 0.437839 1:+33586552 None:intergenic
AATATTGTGGAGTTGAAAAT+GGG 0.451536 1:-33583687 MsaT002184.1:CDS
CAGCTTCAGATTGTGCCATT+GGG 0.460205 1:+33584589 None:intergenic
AGCCAAGCTCAGTGGCAATT+CGG 0.461438 1:-33586554 MsaT002184.1:intron
AAAGAATGCAATGTGGAGAT+TGG 0.464528 1:-33583582 MsaT002184.1:CDS
CAAAGTGGCCATGGTGAAGA+AGG 0.465563 1:-33584622 MsaT002184.1:CDS
GAGCTTGGCTGAACTGCAAT+CGG 0.467944 1:+33586567 None:intergenic
TGTCATTATAACTTCAAAAG+AGG 0.475259 1:+33584554 None:intergenic
TTAACTTTCAAATTCATTGG+TGG 0.475675 1:-33585069 MsaT002184.1:CDS
GGTGCTAAAGCCACTGAAGA+AGG 0.489506 1:-33584970 MsaT002184.1:CDS
ATGAGAGTATTCGTGGCTGC+AGG 0.493889 1:-33583632 MsaT002184.1:CDS
CCAAAGGAGTTTATGAAAAG+AGG 0.496159 1:-33583720 MsaT002184.1:CDS
GTCATTATAACTTCAAAAGA+GGG 0.507540 1:+33584555 None:intergenic
CGTGGCTGCAGGTTCAGTAA+AGG 0.507598 1:-33583621 MsaT002184.1:CDS
AATTATTATGAGAGTATTCG+TGG 0.512630 1:-33583639 MsaT002184.1:CDS
ACTTTCTTTACCTTCTTCAG+TGG 0.514754 1:+33584960 None:intergenic
AAGAGATATCCATATTTGAA+AGG 0.520085 1:+33585090 None:intergenic
AAGCTCAAAGGACAATAAAA+GGG 0.530769 1:-33584858 MsaT002184.1:CDS
AGGATGTCATGAAAATATTG+TGG 0.531604 1:-33583700 MsaT002184.1:CDS
ATATTCCTTAAATTTCCAAA+CGG 0.536219 1:-33585189 MsaT002184.1:CDS
AAAGGGAAGAGAAAATTCTC+AGG 0.557828 1:-33584841 MsaT002184.1:intron
TGAAAATGGGGAGGAAGTCA+TGG 0.563749 1:-33583674 MsaT002184.1:CDS
AACGACACTGCCATGTCTGT+TGG 0.574077 1:+33586593 None:intergenic
GCTGCAGGTTCAGTAAAGGT+TGG 0.575963 1:-33583617 MsaT002184.1:CDS
AGGTAGTAACAAAGTGGCCA+TGG 0.582904 1:-33584631 MsaT002184.1:CDS
GAGGGTTCCTAAGATATATG+TGG 0.586451 1:-33585241 MsaT002184.1:CDS
ACTTACCAAGAAATAGCCAT+TGG 0.587587 1:+33584521 None:intergenic
GTATTTGCAGAGTGCACCAA+AGG 0.590998 1:-33583736 MsaT002184.1:intron
ATGACACAATCTTACACCAA+TGG 0.609025 1:-33584537 MsaT002184.1:CDS
TATGTGGAGAAATATTGGAA+AGG 0.617787 1:-33585225 MsaT002184.1:CDS
AAATGTCAAAGCAAGCTCAA+AGG 0.625671 1:-33584870 MsaT002184.1:CDS
AAGTGATGAAGAGAGTGATG+AGG 0.629681 1:-33584896 MsaT002184.1:CDS
TTGTGGAGTTGAAAATGGGG+AGG 0.632079 1:-33583683 MsaT002184.1:CDS
CTTTACAGGTAGTAACAAAG+TGG 0.648804 1:-33584637 MsaT002184.1:intron
TCATCTTCATCCAACAGACA+TGG 0.668320 1:-33586603 MsaT002184.1:CDS
TGCAGTTCAGCCAAGCTCAG+TGG 0.680189 1:-33586562 MsaT002184.1:CDS
GAAGGCTAGAAAATACCCAA+TGG 0.687317 1:-33584604 MsaT002184.1:CDS
ATATTGTGGAGTTGAAAATG+GGG 0.692849 1:-33583686 MsaT002184.1:CDS
ATTCATGAAAGAATGCAATG+TGG 0.699756 1:-33583589 MsaT002184.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 33583506 33586631 33583506 ID=MsaG002184
Chr1 mRNA 33583506 33586631 33583506 ID=MsaT002184.1;Parent=MsaG002184
Chr1 exon 33583506 33583748 33583506 ID=MsaT002184.1.exon5;Parent=MsaT002184.1
Chr1 CDS 33583506 33583748 33583506 ID=cds.MsaT002184.1;Parent=MsaT002184.1
Chr1 exon 33584527 33584651 33584527 ID=MsaT002184.1.exon4;Parent=MsaT002184.1
Chr1 CDS 33584527 33584651 33584527 ID=cds.MsaT002184.1;Parent=MsaT002184.1
Chr1 exon 33584842 33585262 33584842 ID=MsaT002184.1.exon3;Parent=MsaT002184.1
Chr1 CDS 33584842 33585262 33584842 ID=cds.MsaT002184.1;Parent=MsaT002184.1
Chr1 exon 33585382 33585406 33585382 ID=MsaT002184.1.exon2;Parent=MsaT002184.1
Chr1 CDS 33585382 33585406 33585382 ID=cds.MsaT002184.1;Parent=MsaT002184.1
Chr1 exon 33586555 33586631 33586555 ID=MsaT002184.1.exon1;Parent=MsaT002184.1
Chr1 CDS 33586555 33586631 33586555 ID=cds.MsaT002184.1;Parent=MsaT002184.1
Gene Sequence

>MsaT002184.1

ATGTCTTCATCTTCATCCAACAGACATGGCAGTGTCGTTGCTCCGATTGCAGTTCAGCCAAGCTCAGTGGCAATTCGGATAATCTTGCAAGATAAGAAACTTAGGGTTCCTAAGATATATGTGGAGAAATATTGGAAAGGAATATCAAACCCTATATTCCTTAAATTTCCAAACGGTGTTGAACAAAAAATATTTTGGGTGGAGAGAAATGGTGATATTTGGTTTCAAAAAAACTGGGAAAGTTTTGCAAAGCCTTTCAAATATGGATATCTCTTAACTTTCAAATTCATTGGTGGATCATATTTTAAGGTTAAGATATATGGTGCCAATACTTTAGAAATAAACTATTCCAATATCAAATCAGTTTTTGAAGGTGCTAAAGCCACTGAAGAAGGTAAAGAAAGTGATGAATCAAGTGATGATGAACCCACAAAAGAAGATGAAGAAAGTGATGAAGAGAGTGATGAGGTTGAAATGTCAAAGCAAGCTCAAAGGACAATAAAAGGGAAGAGAAAATTCTCAGGTAGTAACAAAGTGGCCATGGTGAAGAAGGCTAGAAAATACCCAATGGCACAATCTGAAGCTGTTAATGAGAATCCCTCTTTTGAAGTTATAATGACACAATCTTACACCAATGGCTATTTCTTGAGTGCACCAAAGGAGTTTATGAAAAGAGGATGTCATGAAAATATTGTGGAGTTGAAAATGGGGAGGAAGTCATGGTTCGTAAAAGTGAATTATTATGAGAGTATTCGTGGCTGCAGGTTCAGTAAAGGTTGGATAAAATTCATGAAAGAATGCAATGTGGAGATTGGAGATACTTGTCTCTTTAAGTTGATTGATGAGAGCAAGTTTGTGTTTGATGTTTCAATTGTAGGAAAGAACCCATGA

Protein sequence

>MsaT002184.1

MSSSSSNRHGSVVAPIAVQPSSVAIRIILQDKKLRVPKIYVEKYWKGISNPIFLKFPNGVEQKIFWVERNGDIWFQKNWESFAKPFKYGYLLTFKFIGGSYFKVKIYGANTLEINYSNIKSVFEGAKATEEGKESDESSDDEPTKEDEESDEESDEVEMSKQAQRTIKGKRKFSGSNKVAMVKKARKYPMAQSEAVNENPSFEVIMTQSYTNGYFLSAPKEFMKRGCHENIVELKMGRKSWFVKVNYYESIRGCRFSKGWIKFMKECNVEIGDTCLFKLIDESKFVFDVSIVGKNP*