AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002185

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002185 MtrunA17_Chr1g0163201 76.699 309 60 3 2 298 87 395 1.44e-162 458
MsaG002185 MtrunA17_Chr1g0163181 65.318 346 69 5 2 298 89 432 1.15e-148 424
MsaG002185 MtrunA17_Chr1g0163151 72.523 222 61 0 75 296 1 222 2.65e-118 339
MsaG002185 MtrunA17_Chr1g0163111 68.776 237 57 4 75 299 1 232 7.54e-109 315
MsaG002185 MtrunA17_Chr1g0163251 41.152 243 103 4 2 228 89 307 5.31e-48 165
MsaG002185 MtrunA17_Chr6g0476171 40.553 217 124 4 84 295 29 245 3.07e-45 153
MsaG002185 MtrunA17_Chr1g0162151 39.912 228 113 5 75 297 1 209 1.60e-42 145
MsaG002185 MtrunA17_Chr1g0163261 37.198 207 116 2 2 199 93 294 1.75e-36 132
MsaG002185 MtrunA17_Chr1g0163231 41.837 196 98 8 2 189 93 280 3.43e-34 126
MsaG002185 MtrunA17_Chr1g0171611 43.284 201 76 7 2 189 93 268 2.38e-31 118
MsaG002185 MtrunA17_Chr1g0163091 40.559 143 79 2 75 212 1 142 1.53e-29 110
MsaG002185 MtrunA17_Chr1g0163131 39.394 132 73 4 170 297 180 308 2.01e-20 89.4
MsaG002185 MtrunA17_Chr1g0163191 81.690 71 10 1 2 69 89 159 1.74e-18 80.9
MsaG002185 MtrunA17_Chr8g0390841 32.394 142 81 7 61 199 214 343 2.91e-13 70.1
MsaG002185 MtrunA17_Chr3g0131891 27.805 205 124 7 2 188 105 303 1.07e-11 64.7
MsaG002185 MtrunA17_Chr1g0208771 33.333 141 77 5 68 205 181 307 1.83e-11 64.3
MsaG002185 MtrunA17_Chr1g0163241 56.061 66 20 2 2 66 91 148 1.99e-11 61.6
MsaG002185 MtrunA17_Chr7g0233521 30.500 200 120 8 1 188 71 263 9.09e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGGGATTGCTTCGTTTC+CGG 0.165431 1:+33593692 None:intergenic
TTAAAACCTTGTAGTTTCTT+TGG 0.217113 1:+33593675 None:intergenic
ATTGTCCTTTGAGCTTTCTT+TGG 0.254871 1:+33594532 None:intergenic
TTAATGGAACTGTAACAATA+AGG 0.273629 1:-33593893 MsaT002185.1:CDS
TTCATCAACTGATTTGATAT+TGG 0.273749 1:+33594648 None:intergenic
GCATAACACATTCAAGGTTT+TGG 0.294441 1:-33593228 MsaT002185.1:CDS
AAATATTGTGGAGTTGAAAA+TGG 0.298192 1:-33593056 MsaT002185.1:CDS
AAAGCTCAAAGGACAATAAA+TGG 0.303877 1:-33594526 MsaT002185.1:CDS
TAAAACCTTGTAGTTTCTTT+GGG 0.320506 1:+33593676 None:intergenic
CATTAACAGCTTCAGATGTT+TGG 0.351304 1:+33594086 None:intergenic
TAATGGAACTGTAACAATAA+GGG 0.353376 1:-33593892 MsaT002185.1:CDS
GTGGTGGTTGGAAAATATTC+AGG 0.360505 1:-33593803 MsaT002185.1:CDS
GAGGTTAGTTTGAGGTATTA+TGG 0.363534 1:-33593849 MsaT002185.1:CDS
ATAGTGACAGTGAATGAAAA+AGG 0.401426 1:+33593723 None:intergenic
ATAGTTTATTTCTAAAGTAT+TGG 0.406626 1:+33594672 None:intergenic
AGTATTCGTGGCTGCAAGTT+CGG 0.421831 1:-33592995 MsaT002185.1:CDS
TTACAAAGAAAGATCTTGTA+TGG 0.431631 1:-33593826 MsaT002185.1:CDS
AATATTGTGGAGTTGAAAAT+GGG 0.451536 1:-33593055 MsaT002185.1:CDS
CAAAGTCGCCGTGGTGAAGA+AGG 0.454749 1:-33594119 MsaT002185.1:CDS
AAGTGATGAAGAGAGTGATA+AGG 0.461668 1:-33594563 MsaT002185.1:CDS
AAAGAATGCAATGTGGAGAT+TGG 0.464528 1:-33592950 MsaT002185.1:CDS
CGTGGCTGCAAGTTCGGTAA+AGG 0.465915 1:-33592989 MsaT002185.1:CDS
TGTCATTATAACTTCAAAAG+AGG 0.475259 1:+33594057 None:intergenic
GGTGCTGAAGCCACTGAAGA+AGG 0.489506 1:-33594625 MsaT002185.1:CDS
GATGAAGCATAACACATTCA+AGG 0.490908 1:-33593234 MsaT002185.1:CDS
CCAAAGGAATTTATGAAAAG+AGG 0.492978 1:-33593088 MsaT002185.1:CDS
TACTGTACAGAAGGTCATGA+TGG 0.501012 1:-33593257 MsaT002185.1:intron
GTCATTATAACTTCAAAAGA+GGG 0.507540 1:+33594058 None:intergenic
CATGAGGAAGCCACTGAAGA+AGG 0.509106 1:-33594592 MsaT002185.1:CDS
AATTATTATGAGAGTATTCG+TGG 0.512630 1:-33593007 MsaT002185.1:CDS
GGAGTGTGGAGGTTAGTTTG+AGG 0.513468 1:-33593857 MsaT002185.1:CDS
ACTTTCTTTACCTTCTTCAG+TGG 0.514754 1:+33594615 None:intergenic
ACTTTCTTCACCTTCTTCAG+TGG 0.520796 1:+33594582 None:intergenic
AGGATGTCATGGAAATATTG+TGG 0.534496 1:-33593068 MsaT002185.1:CDS
TTTATGAAAAGAGGATGTCA+TGG 0.534842 1:-33593079 MsaT002185.1:CDS
CAATAAGGGTTGGTAATGAA+AGG 0.535875 1:-33593878 MsaT002185.1:CDS
AATGGCAAGAGAAAATTCTC+AGG 0.536390 1:-33594508 MsaT002185.1:intron
AATATCAAATCAGTTGATGA+AGG 0.540855 1:-33594646 MsaT002185.1:CDS
GATCTTGTATGGGTGGTGGT+TGG 0.557083 1:-33593815 MsaT002185.1:CDS
GCTGCAAGTTCGGTAAAGGT+TGG 0.563561 1:-33592985 MsaT002185.1:CDS
TTTGGTGAAGAACACAGCCC+CGG 0.573521 1:-33593210 MsaT002185.1:intron
TGAAAATGGGGAGGAAGCCA+TGG 0.575061 1:-33593042 MsaT002185.1:CDS
GTATTTGCAGAGTGTACCAA+AGG 0.583029 1:-33593104 MsaT002185.1:intron
ACTGTCACTATTACTCGTGC+CGG 0.586569 1:-33593711 MsaT002185.1:CDS
ACTTACAAAGAAATAGCCAT+TGG 0.588354 1:+33594024 None:intergenic
TACAAAGAAAGATCTTGTAT+GGG 0.597334 1:-33593825 MsaT002185.1:CDS
GCAATCCCAAAGAAACTACA+AGG 0.598347 1:-33593681 MsaT002185.1:intron
AAAGAAAGATCTTGTATGGG+TGG 0.602320 1:-33593822 MsaT002185.1:CDS
TGGTGGTTGGAAAATATTCA+GGG 0.603185 1:-33593802 MsaT002185.1:CDS
ATGACACAATCTTACACCAA+TGG 0.609025 1:-33594040 MsaT002185.1:CDS
GGTTGGTAATGAAAGGAGTG+TGG 0.615653 1:-33593871 MsaT002185.1:CDS
GGAACTGTAACAATAAGGGT+TGG 0.627229 1:-33593888 MsaT002185.1:CDS
GAAAGATCTTGTATGGGTGG+TGG 0.630466 1:-33593819 MsaT002185.1:CDS
TTGTGGAGTTGAAAATGGGG+AGG 0.632079 1:-33593051 MsaT002185.1:CDS
TGGTAATGAAAGGAGTGTGG+AGG 0.642373 1:-33593868 MsaT002185.1:CDS
AAATGCCAAAGAAAGCTCAA+AGG 0.663329 1:-33594537 MsaT002185.1:CDS
AGAAGGTAAAGAAAGTCATG+AGG 0.669212 1:-33594608 MsaT002185.1:CDS
ACTGTACAGAAGGTCATGAT+GGG 0.685690 1:-33593256 MsaT002185.1:intron
ATATTGTGGAGTTGAAAATG+GGG 0.692849 1:-33593054 MsaT002185.1:CDS
ATTCATGAAAGAATGCAATG+TGG 0.698838 1:-33592957 MsaT002185.1:CDS
AGGTTGTAACAAAGTCGCCG+TGG 0.726423 1:-33594128 MsaT002185.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 33592874 33594811 33592874 ID=MsaG002185
Chr1 mRNA 33592874 33594811 33592874 ID=MsaT002185.1;Parent=MsaG002185
Chr1 exon 33592874 33593116 33592874 ID=MsaT002185.1.exon6;Parent=MsaT002185.1
Chr1 CDS 33592874 33593116 33592874 ID=cds.MsaT002185.1;Parent=MsaT002185.1
Chr1 exon 33593211 33593269 33593211 ID=MsaT002185.1.exon5;Parent=MsaT002185.1
Chr1 CDS 33593211 33593269 33593211 ID=cds.MsaT002185.1;Parent=MsaT002185.1
Chr1 exon 33593682 33593955 33593682 ID=MsaT002185.1.exon4;Parent=MsaT002185.1
Chr1 CDS 33593682 33593955 33593682 ID=cds.MsaT002185.1;Parent=MsaT002185.1
Chr1 exon 33594030 33594148 33594030 ID=MsaT002185.1.exon3;Parent=MsaT002185.1
Chr1 CDS 33594030 33594148 33594030 ID=cds.MsaT002185.1;Parent=MsaT002185.1
Chr1 exon 33594509 33594710 33594509 ID=MsaT002185.1.exon2;Parent=MsaT002185.1
Chr1 CDS 33594509 33594710 33594509 ID=cds.MsaT002185.1;Parent=MsaT002185.1
Chr1 exon 33594809 33594811 33594809 ID=MsaT002185.1.exon1;Parent=MsaT002185.1
Chr1 CDS 33594809 33594811 33594809 ID=cds.MsaT002185.1;Parent=MsaT002185.1
Gene Sequence

>MsaT002185.1

ATGGTTAAGATATTTGGTGCCAATACTTTAGAAATAAACTATGCCAATATCAAATCAGTTGATGAAGGTGCTGAAGCCACTGAAGAAGGTAAAGAAAGTCATGAGGAAGCCACTGAAGAAGGTGAAGAAAGTGATGAAGAGAGTGATAAGGTTGAAATGCCAAAGAAAGCTCAAAGGACAATAAATGGCAAGAGAAAATTCTCAGGTTGTAACAAAGTCGCCGTGGTGAAGAAGGCTAGAAAATACCAAACATCTGAAGCTGTTAATGAGAATCCCTCTTTTGAAGTTATAATGACACAATCTTACACCAATGGCTATTTCTTTTGGTTACCAAGTGAGTTTTCAAGAGAAAACTTGAAAGATTTTAATGGAACTGTAACAATAAGGGTTGGTAATGAAAGGAGTGTGGAGGTTAGTTTGAGGTATTATGGTTACAAAGAAAGATCTTGTATGGGTGGTGGTTGGAAAATATTCAGGGAAAAATATAATTTGCAAGTTGACGATGTTTGTAAATTTGAGATGATTCGACAGAGACCTTTTTCATTCACTGTCACTATTACTCGTGCCGGAAACGAAGCAATCCCAAAGAAACTACAAGAAGGTCATGATGGGATGAAGCATAACACATTCAAGGTTTTGGTGAAGAACACAGCCCCGAGTGTACCAAAGGAATTTATGAAAAGAGGATGTCATGGAAATATTGTGGAGTTGAAAATGGGGAGGAAGCCATGGTTCGTAAAAGTGAATTATTATGAGAGTATTCGTGGCTGCAAGTTCGGTAAAGGTTGGACAAAATTCATGAAAGAATGCAATGTGGAGATTGGAGATACTTGTCTCTTTAAGTTGATTGATGAGAGAAAATTTGTGTTTGATGTTTCAATTGTTGCAAGAAACCATTAG
Protein sequence

>MsaT002185.1

MVKIFGANTLEINYANIKSVDEGAEATEEGKESHEEATEEGEESDEESDKVEMPKKAQRTINGKRKFSGCNKVAVVKKARKYQTSEAVNENPSFEVIMTQSYTNGYFFWLPSEFSRENLKDFNGTVTIRVGNERSVEVSLRYYGYKERSCMGGGWKIFREKYNLQVDDVCKFEMIRQRPFSFTVTITRAGNEAIPKKLQEGHDGMKHNTFKVLVKNTAPSVPKEFMKRGCHGNIVELKMGRKPWFVKVNYYESIRGCKFGKGWTKFMKECNVEIGDTCLFKLIDERKFVFDVSIVARNH*