AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002187

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002187 MtrunA17_Chr1g0163181 79.048 105 16 1 36 134 334 438 7.70e-52 169
MsaG002187 MtrunA17_Chr1g0163201 79.048 105 16 1 34 132 295 399 1.62e-50 165
MsaG002187 MtrunA17_Chr1g0163151 59.200 125 40 4 12 126 99 222 6.67e-41 135
MsaG002187 MtrunA17_Chr1g0163151 32.800 125 72 4 1 125 1 113 3.80e-11 58.5
MsaG002187 MtrunA17_Chr1g0163111 74.359 78 14 1 56 127 153 230 8.79e-34 117
MsaG002187 MtrunA17_Chr1g0163131 50.617 81 34 2 53 127 228 308 2.46e-17 76.3
MsaG002187 MtrunA17_Chr6g0476171 41.837 98 46 4 37 125 150 245 2.88e-14 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATATCTTGGAGTTGAAAA+TGG 0.201158 1:+33612783 MsaT002187.1:CDS
TTAGCTGTGCTTCTTTATTA+AGG 0.220445 1:+33612967 MsaT002187.1:CDS
CATTAACATCTTCAGATCTT+AGG 0.260861 1:-33611530 None:intergenic
GCATAACACATTCAAGGTTT+TGG 0.294441 1:+33612621 MsaT002187.1:CDS
AATATCTTGGAGTTGAAAAT+GGG 0.359463 1:+33612784 MsaT002187.1:CDS
GTGTTTGATGTTTCAATTGT+TGG 0.364550 1:+33612934 MsaT002187.1:CDS
TAATAAAGAAGCACAGCTAA+TGG 0.396679 1:-33612964 None:intergenic
GGGATATAATGAAAATATCT+TGG 0.418536 1:+33612771 MsaT002187.1:CDS
AAAGAATGCAAAGTGGAGAT+TGG 0.426567 1:+33612871 MsaT002187.1:CDS
ACCAAAGGAGTTTATGAAAA+GGG 0.442898 1:+33612750 MsaT002187.1:CDS
GATGAAGCATAACACATTCA+AGG 0.455053 1:+33612615 MsaT002187.1:CDS
GTGTTATGCTTCATCACATC+AGG 0.468230 1:-33612607 None:intergenic
CGACATTATAACTTCAAAAG+AGG 0.473079 1:-33611559 None:intergenic
TACCAAAGGAGTTTATGAAA+AGG 0.473211 1:+33612749 MsaT002187.1:CDS
TAACAAAGTGGCCATGGTAA+AGG 0.523660 1:+33611494 None:intergenic
GACATTATAACTTCAAAAGA+GGG 0.533539 1:-33611558 None:intergenic
TTTGGTGAAGAACAATGCCC+CGG 0.564050 1:+33612639 MsaT002187.1:CDS
GTATTTGCAGAGTGTACCAA+AGG 0.570521 1:+33612735 MsaT002187.1:intron
AGTGGCTGCAAGTTTAGTAA+AGG 0.571811 1:+33612832 MsaT002187.1:CDS
GCTGCAAGTTTAGTAAAGGA+TGG 0.582172 1:+33612836 MsaT002187.1:CDS
CCAAAGGAGTTTATGAAAAG+GGG 0.584375 1:+33612751 MsaT002187.1:CDS
ATTTATGAAAGAATGCAAAG+TGG 0.597056 1:+33612864 MsaT002187.1:CDS
ATGTCGCAATCTTACGCCAA+TGG 0.602928 1:+33611576 MsaT002187.1:CDS
ACTTACAAAGAAATAGCCAT+TGG 0.605595 1:-33611592 None:intergenic
CAAAGTGGCCATGGTAAAGG+CGG 0.622218 1:+33611497 None:intergenic
TGAAAATGGGGAGAAAGTCA+TGG 0.631530 1:+33612797 MsaT002187.1:CDS
ATATCTTGGAGTTGAAAATG+GGG 0.639227 1:+33612785 MsaT002187.1:CDS
TCATGGTTCGTAAAAGTCAG+TGG 0.652056 1:+33612814 MsaT002187.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 33611507 33612987 33611507 ID=MsaG002187
Chr1 mRNA 33611507 33612987 33611507 ID=MsaT002187.1;Parent=MsaG002187
Chr1 exon 33611507 33611612 33611507 ID=MsaT002187.1.exon1;Parent=MsaT002187.1
Chr1 CDS 33611507 33611612 33611507 ID=cds.MsaT002187.1;Parent=MsaT002187.1
Chr1 exon 33612602 33612660 33612602 ID=MsaT002187.1.exon2;Parent=MsaT002187.1
Chr1 CDS 33612602 33612660 33612602 ID=cds.MsaT002187.1;Parent=MsaT002187.1
Chr1 exon 33612745 33612987 33612745 ID=MsaT002187.1.exon3;Parent=MsaT002187.1
Chr1 CDS 33612745 33612987 33612745 ID=cds.MsaT002187.1;Parent=MsaT002187.1
Gene Sequence

>MsaT002187.1

ATGGTAAAGGCGGCTAGAAAATGCCTAAGATCTGAAGATGTTAATGAGAATCCCTCTTTTGAAGTTATAATGTCGCAATCTTACGCCAATGGCTATTTCTTTGTAAAAGGTCCTGATGTGATGAAGCATAACACATTCAAGGTTTTGGTGAAGAACAATGCCCCGAGTGTACCAAAGGAGTTTATGAAAAGGGGATATAATGAAAATATCTTGGAGTTGAAAATGGGGAGAAAGTCATGGTTCGTAAAAGTCAGTGGCTGCAAGTTTAGTAAAGGATGGATAAAATTTATGAAAGAATGCAAAGTGGAGATTGGAGATATTTGTCTCTTCAAGTTAATTGATGAGAGCAAGTTTGTGTTTGATGTTTCAATTGTTGGAAAGAATCCATTAGCTGTGCTTCTTTATTAA
Protein sequence

>MsaT002187.1

MVKAARKCLRSEDVNENPSFEVIMSQSYANGYFFVKGPDVMKHNTFKVLVKNNAPSVPKEFMKRGYNENILELKMGRKSWFVKVSGCKFSKGWIKFMKECKVEIGDICLFKLIDESKFVFDVSIVGKNPLAVLLY*