AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002189

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002189 MtrunA17_Chr1g0163101 78.788 99 21 0 76 174 1 99 1.38e-53 171
MsaG002189 MtrunA17_Chr1g0163131 67.368 95 31 0 80 174 6 100 1.24e-41 144
MsaG002189 MtrunA17_Chr1g0163131 95.652 69 3 0 2 70 229 297 2.70e-39 138
MsaG002189 MtrunA17_Chr6g0476181 80.882 68 13 0 107 174 1 68 1.83e-36 127
MsaG002189 MtrunA17_Chr1g0162161 82.353 68 12 0 107 174 3 70 3.29e-36 126
MsaG002189 MtrunA17_Chr1g0163141 79.710 69 14 0 106 174 9 77 9.09e-36 125
MsaG002189 MtrunA17_Chr1g0163081 80.882 68 13 0 107 174 5 72 1.78e-34 121
MsaG002189 MtrunA17_Chr1g0163121 75.362 69 17 0 106 174 9 77 6.96e-34 119
MsaG002189 MtrunA17_Chr1g0163161 72.727 55 15 0 120 174 1 55 6.59e-23 90.9
MsaG002189 MtrunA17_Chr1g0171611 37.984 129 62 3 92 202 9 137 3.48e-21 90.1
MsaG002189 MtrunA17_Chr1g0163231 49.398 83 41 1 92 173 9 91 3.03e-20 87.8
MsaG002189 MtrunA17_Chr1g0163261 49.398 83 41 1 92 173 9 91 5.53e-20 87.0
MsaG002189 MtrunA17_Chr1g0163251 52.703 74 34 1 101 173 14 87 9.04e-20 87.8
MsaG002189 MtrunA17_Chr1g0163181 47.126 87 43 2 90 173 1 87 2.62e-18 83.6
MsaG002189 MtrunA17_Chr1g0163181 52.113 71 30 2 2 70 352 420 8.29e-16 76.6
MsaG002189 MtrunA17_Chr1g0163111 58.824 68 24 2 5 70 154 219 7.22e-18 80.1
MsaG002189 MtrunA17_Chr1g0163151 54.412 68 27 2 5 70 147 212 5.25e-17 77.4
MsaG002189 MtrunA17_Chr1g0163241 50.746 67 32 1 108 173 23 89 1.65e-16 75.1
MsaG002189 MtrunA17_Chr1g0163191 45.946 74 39 1 101 173 14 87 4.47e-16 73.6
MsaG002189 MtrunA17_Chr1g0163201 53.521 71 29 2 2 70 315 383 6.11e-16 76.6
MsaG002189 MtrunA17_Chr1g0163201 43.284 67 37 1 108 173 19 85 6.76e-13 67.8
MsaG002189 MtrunA17_Chr6g0467351 41.304 92 50 3 86 173 9 100 1.03e-15 74.3
MsaG002189 MtrunA17_Chr1g0163221 40.860 93 51 2 86 174 5 97 2.86e-15 74.3
MsaG002189 MtrunA17_Chr3g0077411 30.508 177 115 4 5 173 171 347 4.13e-15 74.7
MsaG002189 MtrunA17_Chr1g0163061 43.333 90 47 2 89 174 12 101 1.16e-14 72.0
MsaG002189 MtrunA17_Chr4g0067431 42.391 92 49 3 87 174 10 101 2.19e-14 71.6
MsaG002189 MtrunA17_Chr3g0077461 32.022 178 110 5 5 173 146 321 2.66e-13 69.3
MsaG002189 MtrunA17_Chr7g0233571 38.298 94 55 2 84 174 2 95 9.03e-13 65.9
MsaG002189 MtrunA17_Chr6g0476171 49.231 65 31 1 9 71 173 237 2.88e-12 64.7
MsaG002189 MtrunA17_Chr3g0131891 38.462 91 53 2 87 174 14 104 9.70e-12 63.9
MsaG002189 MtrunA17_Chr1g0162151 51.613 62 21 1 9 70 146 198 1.02e-11 62.8
MsaG002189 MtrunA17_Chr5g0419201 34.951 103 60 3 74 173 2 100 1.39e-11 62.0
MsaG002189 MtrunA17_Chr3g0103151 37.931 87 49 3 90 173 10 94 2.60e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGGTTGGAGAAAGTTTC+AGG 0.242187 1:+33626607 MsaT002189.1:CDS
TTGAATTTGTCGTGTAAATT+CGG 0.303125 1:-33631423 None:intergenic
TGGACGTGGGAGTGAATCTA+AGG 0.326231 1:+33628715 MsaT002189.1:CDS
GAAGAATGCAAATTGAAGTT+TGG 0.337203 1:+33626629 MsaT002189.1:CDS
TACAACGCGGTGAGGATGTT+TGG 0.356017 1:+33629849 MsaT002189.1:CDS
TACCAAGCGAGCTGTTAAAA+AGG 0.361507 1:+33631562 MsaT002189.1:CDS
GGTCCGAGTTCAAAATGAAT+TGG 0.368459 1:+33629825 MsaT002189.1:CDS
TGTTTACTCAAGAACTCATT+TGG 0.374164 1:-33626491 None:intergenic
TTATAACCTTCAATGTGATA+TGG 0.376198 1:-33630544 None:intergenic
AATCTGACCCTCCTTTGTAT+TGG 0.385935 1:-33629928 None:intergenic
AGGATGTTTGGCTTCAGAAT+TGG 0.404471 1:+33629861 MsaT002189.1:CDS
CATTGAAGGTTATAATAGAT+TGG 0.414338 1:+33630552 MsaT002189.1:CDS
ACTCACAAAGTTGAATGGTT+TGG 0.428976 1:-33628751 None:intergenic
TTGCAACTCACAAAGTTGAA+TGG 0.450979 1:-33628756 None:intergenic
AGATTTACATGGAAAATTTG+TGG 0.454668 1:+33626517 MsaT002189.1:CDS
TTATCGCCATATCACATTGA+AGG 0.463256 1:+33630538 MsaT002189.1:CDS
TCAATGTCGAGTGTATACTA+TGG 0.466232 1:+33628695 MsaT002189.1:CDS
TAAAAGAAACTGTGGAGGCC+GGG 0.469744 1:+33630420 MsaT002189.1:CDS
GAATTTACACGACAAATTCA+AGG 0.470517 1:+33631425 MsaT002189.1:CDS
CTGCATAAAATCTACTGCCT+TGG 0.484079 1:-33626589 None:intergenic
CTAAAAGAAACTGTGGAGGC+CGG 0.494656 1:+33630419 MsaT002189.1:CDS
GTCCGAGTTCAAAATGAATT+GGG 0.504162 1:+33629826 MsaT002189.1:CDS
TTTGTGGAGTTGAAAGTTGG+TGG 0.507850 1:+33626533 MsaT002189.1:CDS
GATAATTTGACGGTGAAACT+AGG 0.514346 1:-33630520 None:intergenic
ACATCGCTTAGACTTCCAAA+TGG 0.515725 1:+33629803 MsaT002189.1:CDS
GAAAATTCGTAGAGAGACAT+TGG 0.515869 1:+33629762 MsaT002189.1:CDS
AGTAAACATGAAGATTTACA+TGG 0.517051 1:+33626506 MsaT002189.1:CDS
GCAACCAAGCAAAATGTCTC+AGG 0.523287 1:+33625370 None:intergenic
AAATTTGTGGAGTTGAAAGT+TGG 0.530932 1:+33626530 MsaT002189.1:CDS
TTTGGAAGTCTAAGCGATGT+TGG 0.531226 1:-33629800 None:intergenic
GCACAAATTATCATCCCAAT+GGG 0.541386 1:+33630494 MsaT002189.1:CDS
TGCACAAATTATCATCCCAA+TGG 0.551525 1:+33630493 MsaT002189.1:CDS
ATTTCTTAGCTTTCTTACCC+CGG 0.551754 1:-33630438 None:intergenic
TGAAAGTTGGTGGAAAGTCG+TGG 0.557106 1:+33626543 MsaT002189.1:CDS
CATCGCTTAGACTTCCAAAT+GGG 0.557934 1:+33629804 MsaT002189.1:CDS
AAAAGAAACTGTGGAGGCCG+GGG 0.560094 1:+33630421 MsaT002189.1:CDS
GTGAAACTAGGACTCCCATT+GGG 0.575881 1:-33630508 None:intergenic
GGTGAAACTAGGACTCCCAT+TGG 0.578470 1:-33630509 None:intergenic
TTTGCGCTCTCTCTCAGATG+TGG 0.586381 1:+33629890 MsaT002189.1:CDS
TCGAGTGTATACTATGGACG+TGG 0.590809 1:+33628701 MsaT002189.1:CDS
AAATTGAATTATTATCTCCA+AGG 0.594943 1:+33626572 MsaT002189.1:CDS
GTGATATGGCGATAATTTGA+CGG 0.598386 1:-33630530 None:intergenic
TTTGGCTTCAGAATTGGAAG+CGG 0.599388 1:+33629867 MsaT002189.1:CDS
GGACGTGGGAGTGAATCTAA+GGG 0.633202 1:+33628716 MsaT002189.1:CDS
GCAGAGGCTAAAAGAAACTG+TGG 0.652399 1:+33630412 MsaT002189.1:intron
GAATTGGGTACAACGCGGTG+AGG 0.673958 1:+33629841 MsaT002189.1:CDS
GAGGCTAAAAGAAACTGTGG+AGG 0.695999 1:+33630415 MsaT002189.1:intron
CGAGTGTATACTATGGACGT+GGG 0.696374 1:+33628702 MsaT002189.1:CDS
AAAATGAATTGGGTACAACG+CGG 0.771281 1:+33629836 MsaT002189.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 33625383 33631589 33625383 ID=MsaG002189
Chr1 mRNA 33625383 33631589 33625383 ID=MsaT002189.1;Parent=MsaG002189
Chr1 exon 33625383 33625391 33625383 ID=MsaT002189.1.exon1;Parent=MsaT002189.1
Chr1 CDS 33625383 33625391 33625383 ID=cds.MsaT002189.1;Parent=MsaT002189.1
Chr1 exon 33626485 33626688 33626485 ID=MsaT002189.1.exon2;Parent=MsaT002189.1
Chr1 CDS 33626485 33626688 33626485 ID=cds.MsaT002189.1;Parent=MsaT002189.1
Chr1 exon 33628686 33628790 33628686 ID=MsaT002189.1.exon3;Parent=MsaT002189.1
Chr1 CDS 33628686 33628790 33628686 ID=cds.MsaT002189.1;Parent=MsaT002189.1
Chr1 exon 33629752 33629955 33629752 ID=MsaT002189.1.exon4;Parent=MsaT002189.1
Chr1 CDS 33629752 33629955 33629752 ID=cds.MsaT002189.1;Parent=MsaT002189.1
Chr1 exon 33630416 33630573 33630416 ID=MsaT002189.1.exon5;Parent=MsaT002189.1
Chr1 CDS 33630416 33630573 33630416 ID=cds.MsaT002189.1;Parent=MsaT002189.1
Chr1 exon 33631409 33631470 33631409 ID=MsaT002189.1.exon6;Parent=MsaT002189.1
Chr1 CDS 33631409 33631470 33631409 ID=cds.MsaT002189.1;Parent=MsaT002189.1
Chr1 exon 33631552 33631589 33631552 ID=MsaT002189.1.exon7;Parent=MsaT002189.1
Chr1 CDS 33631552 33631589 33631552 ID=cds.MsaT002189.1;Parent=MsaT002189.1
Gene Sequence

>MsaT002189.1

ATGTCTCAGCGTGTACCAAATGAGTTCTTGAGTAAACATGAAGATTTACATGGAAAATTTGTGGAGTTGAAAGTTGGTGGAAAGTCGTGGTTTGTAAAATTGAATTATTATCTCCAAGGCAGTAGATTTTATGCAGGTTGGAGAAAGTTTCAGGAAGAATGCAAATTGAAGTTTGGAGATATTTGTTTGTTTGAATTAATTGATGAGAAAAATAAGCAAGCATCAATGTCGAGTGTATACTATGGACGTGGGAGTGAATCTAAGGGAATGCATTTTTACCAAACCATTCAACTTTGTGAGTTGCAAGATGATGAGCTTATGATTCCAAGAAAATTCGTAGAGAGACATTGGAAAGATGTATCAAATCCAACATCGCTTAGACTTCCAAATGGGTCCGAGTTCAAAATGAATTGGGTACAACGCGGTGAGGATGTTTGGCTTCAGAATTGGAAGCGGTTTGCGCTCTCTCTCAGATGTGGAGATCTTTTAGTTTTCCAATACAAAGGAGGGTCAGATTTTCATAGGCTAAAAGAAACTGTGGAGGCCGGGGTAAGAAAGCTAAGAAATGCACAAAAACTAAAGATAGGAATGAGAAAAGTTGCACAAATTATCATCCCAATGGGAGTCCTAGTTTCACCGTCAAATTATCGCCATATCACATTGAAGGTTATAATAGATTGCAGATTCTGTAAGTCCGAATTTACACGACAAATTCAAGGTTTTTGTAACATCATTAGACAAGTTGGTTGTACTACCAAGCGAGCTGTTAAAAAGGCATAA
Protein sequence

>MsaT002189.1

MSQRVPNEFLSKHEDLHGKFVELKVGGKSWFVKLNYYLQGSRFYAGWRKFQEECKLKFGDICLFELIDEKNKQASMSSVYYGRGSESKGMHFYQTIQLCELQDDELMIPRKFVERHWKDVSNPTSLRLPNGSEFKMNWVQRGEDVWLQNWKRFALSLRCGDLLVFQYKGGSDFHRLKETVEAGVRKLRNAQKLKIGMRKVAQIIIPMGVLVSPSNYRHITLKVIIDCRFCKSEFTRQIQGFCNIIRQVGCTTKRAVKKA*