AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002191

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002191 MtrunA17_Chr1g0171711 96.692 786 9 2 1 769 1 786 0.0 1569
MsaG002191 MtrunA17_Chr7g0237271 62.643 787 265 12 2 769 8 784 0.0 919
MsaG002191 MtrunA17_Chr7g0237241 61.450 786 267 11 2 769 8 775 0.0 887
MsaG002191 MtrunA17_Chr3g0138311 54.695 426 158 7 369 769 482 897 1.26e-146 452
MsaG002191 MtrunA17_Chr3g0138311 76.136 88 21 0 1 88 1 88 4.12e-39 155
MsaG002191 MtrunA17_Chr2g0282401 50.904 332 137 6 460 769 461 788 6.69e-106 342
MsaG002191 MtrunA17_Chr4g0062881 42.581 465 206 10 324 769 35 457 1.64e-101 320
MsaG002191 MtrunA17_Chr4g0047051 41.971 274 140 4 514 769 36 308 1.67e-63 214
MsaG002191 MtrunA17_Chr1g0148791 39.953 428 138 19 1 340 1 397 9.49e-56 206
MsaG002191 MtrunA17_Chr4g0047071 39.223 283 142 8 514 769 3 282 2.13e-55 192
MsaG002191 MtrunA17_Chr4g0047091 39.298 285 146 6 507 769 87 366 7.37e-52 184
MsaG002191 MtrunA17_Chr4g0047061 41.736 242 134 5 531 769 93 330 5.20e-51 181
MsaG002191 MtrunA17_Chr4g0047101 38.247 251 119 6 495 722 11 248 4.68e-40 148
MsaG002191 MtrunA17_Chr4g0047111 38.514 148 67 3 576 722 7 131 1.27e-27 109
MsaG002191 MtrunA17_Chr4g0062891 38.667 150 85 4 12 155 22 170 1.45e-26 108
MsaG002191 MtrunA17_Chr1g0172501 26.351 296 188 11 496 768 264 552 5.68e-20 94.7
MsaG002191 MtrunA17_Chr3g0093681 26.351 296 188 11 496 768 264 552 7.60e-20 94.0
MsaG002191 MtrunA17_Chr5g0405851 25.806 310 187 13 496 768 146 449 1.11e-17 86.7
MsaG002191 MtrunA17_Chr6g0448921 25.728 206 146 5 533 734 58 260 1.57e-17 87.4
MsaG002191 MtrunA17_Chr6g0448921 23.529 204 151 3 570 768 9 212 1.99e-12 70.9
MsaG002191 MtrunA17_Chr1g0163021 50.000 68 29 2 662 724 21 88 3.76e-16 75.1
MsaG002191 MtrunA17_Chr8g0352821 25.123 203 142 7 574 768 264 464 1.10e-15 81.3
MsaG002191 MtrunA17_Chr5g0401661 32.044 181 107 7 539 706 484 661 2.08e-15 80.5
MsaG002191 MtrunA17_Chr5g0401661 23.077 247 170 9 537 768 399 640 2.23e-11 67.4
MsaG002191 MtrunA17_Chr4g0053651 31.492 181 108 7 539 706 377 554 1.97e-14 77.0
MsaG002191 MtrunA17_Chr4g0053651 23.774 265 182 10 519 768 274 533 2.85e-13 73.2
MsaG002191 MtrunA17_Chr4g0053651 24.900 249 154 8 516 735 297 541 5.26e-11 66.2
MsaG002191 MtrunA17_Chr5g0401671 28.177 181 114 6 539 706 394 571 2.69e-14 76.6
MsaG002191 MtrunA17_Chr2g0281451 24.390 205 148 5 570 768 8 211 3.01e-14 77.0
MsaG002191 MtrunA17_Chr2g0281451 24.468 188 136 4 533 716 57 242 2.45e-12 70.9
MsaG002191 MtrunA17_Chr4g0067151 25.517 290 176 12 516 768 35 321 3.34e-14 74.7
MsaG002191 MtrunA17_Chr3g0136971 24.286 280 200 9 497 768 339 614 3.23e-13 73.2
MsaG002191 MtrunA17_Chr1g0185501 23.265 245 177 8 533 768 26 268 7.88e-13 70.5
MsaG002191 MtrunA17_Chr1g0168391 29.310 174 111 6 520 687 256 423 2.64e-12 70.1
MsaG002191 MtrunA17_Chr4g0059031 26.178 191 125 6 592 768 34 222 7.96e-12 68.9
MsaG002191 MtrunA17_Chr2g0295791 25.352 213 151 5 560 767 155 364 9.97e-12 68.2
MsaG002191 MtrunA17_Chr7g0253861 21.986 282 188 8 516 767 63 342 4.08e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002191 AT2G32700.2 71.465 792 198 13 1 769 1 787 0.0 1055
MsaG002191 AT2G32700.5 71.465 792 198 13 1 769 1 787 0.0 1055
MsaG002191 AT2G32700.1 71.465 792 198 13 1 769 1 787 0.0 1055
MsaG002191 AT2G32700.4 71.465 792 198 13 1 769 1 787 0.0 1055
MsaG002191 AT2G32700.3 71.465 792 198 13 1 769 1 787 0.0 1055
MsaG002191 AT2G32700.6 71.212 792 198 14 1 769 1 785 0.0 1046
MsaG002191 AT2G32700.7 69.790 811 198 14 1 769 1 806 0.0 1044
MsaG002191 AT4G32551.1 57.583 422 147 7 369 769 521 931 4.38e-157 481
MsaG002191 AT4G32551.1 77.273 88 20 0 1 88 1 88 7.31e-40 159
MsaG002191 AT4G32551.1 35.096 208 98 10 107 291 186 379 5.37e-13 73.2
MsaG002191 AT4G32551.2 57.583 422 147 7 369 769 559 969 1.28e-156 480
MsaG002191 AT4G32551.2 77.273 88 20 0 1 88 1 88 8.08e-40 159
MsaG002191 AT4G32551.2 32.627 236 106 11 106 291 185 417 1.66e-11 68.6
MsaG002191 AT2G41500.1 27.857 280 172 11 514 768 261 535 6.41e-20 94.7
MsaG002191 AT2G41500.2 27.857 280 172 11 514 768 261 535 6.41e-20 94.7
MsaG002191 AT3G49660.1 25.342 292 184 11 508 768 24 312 1.46e-15 79.0
MsaG002191 AT1G61210.1 26.341 205 144 5 570 768 8 211 2.86e-15 80.9
MsaG002191 AT1G61210.1 25.381 197 139 5 555 745 37 231 6.01e-11 67.0
MsaG002191 AT1G61210.3 26.341 205 144 5 570 768 8 211 3.32e-15 80.5
MsaG002191 AT1G61210.3 25.381 197 139 5 555 745 37 231 7.61e-11 66.6
MsaG002191 AT1G61210.2 26.341 205 144 5 570 768 8 211 3.36e-15 80.5
MsaG002191 AT1G61210.2 25.381 197 139 5 555 745 37 231 7.25e-11 66.6
MsaG002191 AT4G02730.1 25.000 300 195 11 498 769 33 330 4.63e-15 77.8
MsaG002191 AT5G13480.3 26.108 203 140 7 574 768 192 392 1.05e-14 78.6
MsaG002191 AT5G13480.4 26.108 203 140 7 574 768 192 392 1.08e-14 78.6
MsaG002191 AT5G13480.1 26.108 203 140 7 574 768 204 404 1.13e-14 78.6
MsaG002191 AT5G13480.2 26.108 203 140 7 574 768 215 415 1.38e-14 78.2
MsaG002191 AT1G11160.1 25.366 205 146 5 570 768 8 211 2.71e-14 77.8
MsaG002191 AT1G11160.4 25.366 205 146 5 570 768 8 211 3.24e-14 77.4
MsaG002191 AT1G11160.2 25.366 205 146 5 570 768 8 211 3.24e-14 77.4
MsaG002191 AT5G08390.1 24.020 204 150 3 570 768 9 212 5.01e-13 73.6
MsaG002191 AT5G08390.1 26.425 193 132 8 543 729 26 214 1.10e-11 68.9
MsaG002191 AT1G11160.3 23.404 188 138 4 533 716 15 200 5.63e-13 73.2
MsaG002191 AT5G25150.1 23.529 238 139 6 533 768 417 613 1.04e-12 72.0
MsaG002191 AT2G33340.2 28.409 176 112 6 520 687 254 423 1.32e-12 71.6
MsaG002191 AT2G33340.1 28.409 176 112 6 520 687 254 423 1.32e-12 71.6
MsaG002191 AT5G25150.2 23.529 238 139 6 533 768 417 613 1.36e-12 71.6
MsaG002191 AT5G67320.1 23.746 299 210 8 484 768 274 568 1.62e-12 71.2
MsaG002191 AT2G33340.3 28.409 176 112 6 520 687 254 423 1.64e-12 70.9
MsaG002191 AT5G23430.3 24.390 205 148 5 570 768 9 212 3.24e-12 70.9
MsaG002191 AT5G23430.2 24.390 205 148 5 570 768 9 212 3.24e-12 70.9
MsaG002191 AT5G23430.1 24.390 205 148 5 570 768 9 212 3.30e-12 70.9
MsaG002191 AT1G29260.1 25.000 228 158 8 555 769 82 309 4.81e-12 68.2
MsaG002191 AT2G43770.1 20.906 287 195 8 511 767 56 340 3.67e-11 65.9

Find 195 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAGGGTTTGATGATTTA+TGG 0.148294 1:-33681811 MsaT002191.1:CDS
CAGGATGGCTTGGAACATTT+TGG 0.166410 1:-33681593 MsaT002191.1:CDS
TGTTTACATATATGATTATT+TGG 0.180095 1:-33686567 MsaT002191.1:CDS
AAACTATGGCGCAGAGTAAT+TGG 0.185140 1:-33686706 None:intergenic
CCTGTGGATCCTTCAATTTA+TGG 0.198445 1:-33685596 MsaT002191.1:CDS
GGATACAAATGGAGATTATT+TGG 0.212936 1:-33673719 MsaT002191.1:CDS
GGTGATGGAAAGGACTTATT+TGG 0.218195 1:-33681518 MsaT002191.1:CDS
GCAGCCAAGCCTTTCTTTGC+AGG 0.229828 1:-33674226 MsaT002191.1:intron
AGAACAAAACTCACAGTTAA+AGG 0.231736 1:+33685219 None:intergenic
GAACAAATTCGCCCAGGTTT+TGG 0.251224 1:-33685130 MsaT002191.1:CDS
TTGATACAACTGTGCGGCTT+TGG 0.280722 1:-33677106 MsaT002191.1:CDS
CACCTTCTGGCCGCAGCATC+TGG 0.284735 1:-33673942 MsaT002191.1:CDS
TATTATAGAATAACCGAAAA+AGG 0.301943 1:-33681981 MsaT002191.1:intron
CTGTGGATCCTTCAATTTAT+GGG 0.314347 1:-33685595 MsaT002191.1:CDS
TACTCCAACCTCTTGGTTAT+CGG 0.325693 1:-33673586 MsaT002191.1:CDS
CCCTTCTTTGCATCTGAGCT+TGG 0.331207 1:+33686135 None:intergenic
GCCAAGGAGTGGACACCTTC+TGG 0.333667 1:-33673955 MsaT002191.1:CDS
GTTGCCAGCTGAGTTGAATT+TGG 0.340739 1:+33677137 None:intergenic
CAGGCAAATGCACTCATTAC+AGG 0.344223 1:-33673886 MsaT002191.1:intron
GCATTCACAGCAACTGGTTC+AGG 0.351607 1:-33682639 MsaT002191.1:intron
AGGAAAGGGCCTACATCTTC+AGG 0.354520 1:-33681961 MsaT002191.1:CDS
AATGCAATCTACTTCATCTA+AGG 0.355132 1:-33684885 MsaT002191.1:intron
TCCTATATCAGGATTAAATC+AGG 0.359023 1:-33685271 MsaT002191.1:intron
GAAACTGATTTGCACTTTGA+AGG 0.360965 1:+33685086 None:intergenic
CGCTATATGCCTGCAAAGAA+AGG 0.364543 1:+33674217 None:intergenic
GGGAGGTACATTGTGTATGC+TGG 0.368192 1:-33673741 MsaT002191.1:CDS
TGCTGTCATTTCTCTTCAGA+TGG 0.369775 1:-33681328 MsaT002191.1:CDS
GTACATGCACAAGGAAATAT+TGG 0.369935 1:-33685022 MsaT002191.1:CDS
TCCTTTGCATTCACAGCAAC+TGG 0.371305 1:-33682645 MsaT002191.1:CDS
GTTCCAATGGGAGGATGATT+AGG 0.373960 1:+33686110 None:intergenic
GCAATCTACTGCCACTGCTT+TGG 0.375891 1:-33686048 MsaT002191.1:CDS
TGATTCCACATTATCATCTA+AGG 0.380642 1:+33681559 None:intergenic
TGATACAACTGTGCGGCTTT+GGG 0.391565 1:-33677105 MsaT002191.1:CDS
AGCAGCTTTGCAGCAAATGC+AGG 0.394616 1:-33685793 MsaT002191.1:CDS
GAATCTGTAGCATGCTCGGC+AGG 0.396589 1:+33681479 None:intergenic
ATAACAATGAGATTCGCTTC+TGG 0.401253 1:-33674125 MsaT002191.1:CDS
GATGATTTGTTGATTTCAAA+AGG 0.402681 1:+33685937 None:intergenic
GTCAAGAATCTGTCAAAGTT+TGG 0.406905 1:-33673687 MsaT002191.1:CDS
TGAACTTGAGCTCCAAAACC+TGG 0.407070 1:+33685118 None:intergenic
CTGTCACTGGTGATTGTGCT+AGG 0.409504 1:+33673011 None:intergenic
ATGATAAGTCGGTAAAGATA+TGG 0.410149 1:-33672967 MsaT002191.1:CDS
GGAGGATTGCAGTCAAAGTC+TGG 0.414299 1:-33685569 MsaT002191.1:CDS
TTCACAAGTGTATGGAGATA+TGG 0.414553 1:-33684996 MsaT002191.1:CDS
GGCAACTTGCAGAATGTTGC+TGG 0.416007 1:-33681838 MsaT002191.1:CDS
TCACCAGGAGTATGAATAGA+TGG 0.426192 1:+33681877 None:intergenic
ATTTCCTGTACCAGTACTAT+TGG 0.429601 1:+33681933 None:intergenic
ACCAAGAGGTTGGAGTAACT+TGG 0.432164 1:+33673592 None:intergenic
AAAGTTTGGTCACTTGCCTC+TGG 0.432801 1:-33673673 MsaT002191.1:CDS
TTGATTTACCAGCTGGTTTG+TGG 0.439336 1:+33681759 None:intergenic
TAATGCCGCAGCATATTTAG+AGG 0.440510 1:-33686291 MsaT002191.1:intron
ATTGACCCATCGTTTGCAAT+TGG 0.440613 1:+33684917 None:intergenic
GTTGACGGTTGAGAATTAGA+AGG 0.441908 1:+33681901 None:intergenic
ATGTATTTCTAGGGACACTC+TGG 0.444218 1:-33673763 MsaT002191.1:intron
GCCAGAAGGTGTCCACTCCT+TGG 0.444368 1:+33673954 None:intergenic
ATTGCAAACGATGGGTCAAT+AGG 0.445275 1:-33684914 MsaT002191.1:CDS
AACTATGGCGCAGAGTAATT+GGG 0.449762 1:-33686705 None:intergenic
CCTCCCTGCCCATAAATTGA+AGG 0.450930 1:+33685587 None:intergenic
TTGAAATCAACAAATCATCC+TGG 0.452186 1:-33685933 MsaT002191.1:CDS
GTGATGGAAAGGACTTATTT+GGG 0.454425 1:-33681517 MsaT002191.1:CDS
CATGCTACAGATTCTTCAAA+AGG 0.454429 1:-33681470 MsaT002191.1:intron
GCAAAGGATCATGTTGTTGC+TGG 0.454859 1:+33682660 None:intergenic
ATTGGCAATTCACAAGTGTA+TGG 0.462672 1:-33685004 MsaT002191.1:CDS
GATGATGTCTCTGTATCCAT+AGG 0.464780 1:+33685987 None:intergenic
GGATCCTTCAATTTATGGGC+AGG 0.467209 1:-33685591 MsaT002191.1:CDS
TCTGTAGTGATAGCATTTAG+AGG 0.468147 1:+33686083 None:intergenic
CTTCTTGTCATGTCCAGCAC+TGG 0.468790 1:+33681294 None:intergenic
CCCAAGTTACTCCAACCTCT+TGG 0.475839 1:-33673593 MsaT002191.1:CDS
ATGGATCCTCAACGATTAAG+AGG 0.479813 1:-33684977 MsaT002191.1:CDS
ACTTATTTGGGACGTTGAAA+CGG 0.485085 1:-33681505 MsaT002191.1:CDS
ACCATAAATCATCAAACCCT+TGG 0.489033 1:+33681810 None:intergenic
TACACCCTCATGAGCTGGTA+TGG 0.493721 1:+33673042 None:intergenic
GCCAAGCTCAGATGCAAAGA+AGG 0.495289 1:-33686136 MsaT002191.1:CDS
CTAAACAGTCAGATGGTTCA+AGG 0.495740 1:-33685834 MsaT002191.1:intron
TTCTAGCATCTAAAAGTGGT+TGG 0.500545 1:+33685964 None:intergenic
CTTGGTTATCGGAGGATACC+AGG 0.501060 1:-33673575 MsaT002191.1:intron
TCTAGCATCTAAAAGTGGTT+GGG 0.502456 1:+33685965 None:intergenic
ACTCACTCACCTACTGGATC+AGG 0.504721 1:+33686488 None:intergenic
TCTTTGATGTTGAGACTGAC+AGG 0.507574 1:-33673905 MsaT002191.1:CDS
TGAAGAATCTGTAGCATGCT+CGG 0.510849 1:+33681475 None:intergenic
GCTGTCATTTCTCTTCAGAT+GGG 0.513862 1:-33681327 MsaT002191.1:CDS
CAGACCAAATTCAACTCAGC+TGG 0.516977 1:-33677141 MsaT002191.1:CDS
GGATTAAATCAGGGAGTTGG+TGG 0.517163 1:-33685261 MsaT002191.1:CDS
GTCATTTATGACAGAAGGGA+AGG 0.518397 1:-33686516 MsaT002191.1:CDS
ATCATGTTGTTGCTGGGAGA+TGG 0.518697 1:+33682667 None:intergenic
TCAGGAGCTGCCAATAGTAC+TGG 0.519807 1:-33681943 MsaT002191.1:CDS
TTGATGATTTATGGTACTGA+AGG 0.523817 1:-33681802 MsaT002191.1:CDS
TTAGATGAAGTAGATTGCAT+TGG 0.524357 1:+33684887 None:intergenic
GCTAAGTCATTTATGACAGA+AGG 0.525516 1:-33686521 MsaT002191.1:CDS
TGCCAGATGCTGCGGCCAGA+AGG 0.525651 1:+33673940 None:intergenic
AAGTCTGGAATCACAAATGC+AGG 0.526168 1:-33685554 MsaT002191.1:intron
CTTTACCGACTTATCATGAC+TGG 0.527534 1:+33672973 None:intergenic
TCCTGGTGATGGAGTTGCTA+TGG 0.529700 1:-33681863 MsaT002191.1:CDS
TGCATCTTCCACAAACCAGC+TGG 0.530085 1:-33681767 MsaT002191.1:intron
ATGATTTGTTGATTTCAAAA+GGG 0.530464 1:+33685938 None:intergenic
AATCTCATTGTTATCATCAC+AGG 0.531071 1:+33674134 None:intergenic
GGGAAATTATTAGCCAGTGC+TGG 0.533775 1:-33681307 MsaT002191.1:CDS
CTAGCACAATCACCAGTGAC+AGG 0.536452 1:-33673010 MsaT002191.1:CDS
ACAACAACAGCTCCTTGCTC+AGG 0.536955 1:-33685044 MsaT002191.1:CDS
GAAGCAACCATCCCTGTCAC+TGG 0.538448 1:+33672998 None:intergenic
TGATTTGCACTTTGAAGGAG+AGG 0.539847 1:+33685091 None:intergenic
GTATGAATAGATGGAGTTGA+CGG 0.541616 1:+33681886 None:intergenic
TGTGTATGCTGGGATACAAA+TGG 0.541891 1:-33673730 MsaT002191.1:CDS
GCTGCCAATAGTACTGGTAC+AGG 0.543266 1:-33681937 MsaT002191.1:CDS
TGAAATCAACAAATCATCCT+GGG 0.545342 1:-33685932 MsaT002191.1:intron
ACTTACAGTCATTGGAGCTA+TGG 0.548084 1:-33673090 MsaT002191.1:intron
TTTAGAGGAGGAGTTCCAAT+GGG 0.550042 1:+33686098 None:intergenic
ATCACTACAGAAGGAATGCT+AGG 0.550461 1:-33686071 MsaT002191.1:CDS
TCTATTCATACTCCTGGTGA+TGG 0.551044 1:-33681874 MsaT002191.1:CDS
GTGGTCTCACATTGAAAGGA+TGG 0.551071 1:-33685242 MsaT002191.1:CDS
TCATTTCTCTCACAAGATGA+TGG 0.552374 1:-33681539 MsaT002191.1:CDS
GATCCTAATCATCCTCCCAT+TGG 0.553131 1:-33686113 MsaT002191.1:CDS
ATTTAGAGGAGGAGTTCCAA+TGG 0.557133 1:+33686097 None:intergenic
AGAATGAAACATGTTTCCAC+TGG 0.557652 1:+33673627 None:intergenic
GGAGGTACATTGTGTATGCT+GGG 0.558903 1:-33673740 MsaT002191.1:CDS
TTTCTTAGGGTGGAAATCAA+GGG 0.559171 1:+33674173 None:intergenic
TCCTCCGATAACCAAGAGGT+TGG 0.560162 1:+33673582 None:intergenic
TCAGGATTAAATCAGGGAGT+TGG 0.560797 1:-33685264 MsaT002191.1:intron
TATGCCTGCAAAGAAAGGCT+TGG 0.561064 1:+33674222 None:intergenic
TATCATCACAGGAACAGAAA+AGG 0.561084 1:+33674145 None:intergenic
TGTATTTCTAGGGACACTCT+GGG 0.561169 1:-33673762 MsaT002191.1:intron
ACTCCATCTATTCATACTCC+TGG 0.561698 1:-33681880 MsaT002191.1:CDS
CCAAGAGGTTGGAGTAACTT+GGG 0.561716 1:+33673593 None:intergenic
GAACTTGAGCTCCAAAACCT+GGG 0.565296 1:+33685119 None:intergenic
TATGACCATACCAGCTCATG+AGG 0.566508 1:-33673047 MsaT002191.1:CDS
GATATTACACCCTCATGAGC+TGG 0.567462 1:+33673037 None:intergenic
CTAAATGCTATCACTACAGA+AGG 0.567631 1:-33686080 MsaT002191.1:CDS
TGTCACTGGTGATTGTGCTA+GGG 0.568713 1:+33673012 None:intergenic
GTCAACCAATTGCAAACGAT+GGG 0.570901 1:-33684922 MsaT002191.1:CDS
AATTGAAGGATCCACAGGTA+AGG 0.571880 1:+33685601 None:intergenic
CTCTCACAAGATGATGGTGA+TGG 0.571976 1:-33681533 MsaT002191.1:CDS
GTCATTGGAGCTATGGCACA+TGG 0.572018 1:-33673083 MsaT002191.1:CDS
ATTTATGGTACTGAAGGAGC+AGG 0.572820 1:-33681796 MsaT002191.1:CDS
GGCATAGAACAAATTCGCCC+AGG 0.572846 1:-33685136 MsaT002191.1:CDS
CCATAAATTGAAGGATCCAC+AGG 0.574667 1:+33685596 None:intergenic
GAGATGGATTGTTGCATCTG+TGG 0.577117 1:+33682683 None:intergenic
TATCAAAAGCGAAGTCAACA+TGG 0.577237 1:-33685639 MsaT002191.1:CDS
CACAACATTGCCAGATGCTG+CGG 0.578567 1:+33673932 None:intergenic
ACAAGATGATGGTGATGGAA+AGG 0.579632 1:-33681528 MsaT002191.1:CDS
TTAAGAGGATTAGCTAGAGG+AGG 0.579927 1:-33684962 MsaT002191.1:CDS
GAATGTTGCTGGTGTATCCA+AGG 0.582149 1:-33681827 MsaT002191.1:CDS
ATTCATGAACTTAATTCCAG+TGG 0.584603 1:-33673643 MsaT002191.1:CDS
TTGGCAGCTCCTGAAGATGT+AGG 0.588055 1:+33681952 None:intergenic
GATCCTTCAATTTATGGGCA+GGG 0.588175 1:-33685590 MsaT002191.1:CDS
CTTGTGCATGTACCTGAGCA+AGG 0.588469 1:+33685032 None:intergenic
CACAGGTGAGGTTTCAGCCA+AGG 0.590433 1:-33673971 MsaT002191.1:CDS
CCAAGCTCAGATGCAAAGAA+GGG 0.590704 1:-33686135 MsaT002191.1:CDS
ATGATGTCTCTGTATCCATA+GGG 0.590798 1:+33685988 None:intergenic
CCTATATCAGGATTAAATCA+GGG 0.591636 1:-33685270 MsaT002191.1:intron
GTTGGTGGTCTCACATTGAA+AGG 0.592499 1:-33685246 MsaT002191.1:CDS
AATGCAGCAATTGATGCTCC+TGG 0.595005 1:-33686386 MsaT002191.1:intron
TCCAACCTCTTGGTTATCGG+AGG 0.596783 1:-33673583 MsaT002191.1:CDS
GCCATAGCAACTCCATCACC+AGG 0.598806 1:+33681862 None:intergenic
CTAAGTCATTTATGACAGAA+GGG 0.600445 1:-33686520 MsaT002191.1:CDS
TTGACGGTTGAGAATTAGAA+GGG 0.600579 1:+33681902 None:intergenic
AATGTTGCTGGTGTATCCAA+GGG 0.601732 1:-33681826 MsaT002191.1:CDS
GTGAGGTTTCAGCCAAGGAG+TGG 0.601900 1:-33673966 MsaT002191.1:CDS
TATAGAACTAACTTCACTGA+AGG 0.604171 1:+33681375 None:intergenic
GCCATTCTAGCATCTAAAAG+TGG 0.604873 1:+33685960 None:intergenic
CTTTCTTTGCAGGCATATAG+CGG 0.607957 1:-33674216 MsaT002191.1:CDS
GGTGATGGAGTTGCTATGGC+GGG 0.608534 1:-33681859 MsaT002191.1:CDS
AGTTCTATACGCAAAAGCAA+TGG 0.612132 1:-33681361 MsaT002191.1:CDS
CAAAGGATCATGTTGTTGCT+GGG 0.617599 1:+33682661 None:intergenic
TGGTGATGGAGTTGCTATGG+CGG 0.619692 1:-33681860 MsaT002191.1:CDS
GCTAATCCTCTTAATCGTTG+AGG 0.619752 1:+33684971 None:intergenic
TTCTGCCAGTCATGATAAGT+CGG 0.620023 1:-33672978 MsaT002191.1:CDS
CAGTGCTGGACATGACAAGA+AGG 0.620953 1:-33681293 MsaT002191.1:intron
CTTGCTCAGGTACATGCACA+AGG 0.621214 1:-33685031 MsaT002191.1:CDS
CACTCGAAAAGAAAACCACC+AGG 0.623943 1:+33686368 None:intergenic
CGATTAAGAGGATTAGCTAG+AGG 0.624323 1:-33684965 MsaT002191.1:CDS
CCTTCAATTTATGGGCAGGG+AGG 0.628421 1:-33685587 MsaT002191.1:CDS
ATCAAAAGCGAAGTCAACAT+GGG 0.635563 1:-33685638 MsaT002191.1:CDS
ATTTGGTCTGAAGCGAACGT+CGG 0.638102 1:+33677154 None:intergenic
AGTCAACCAATTGCAAACGA+TGG 0.639225 1:-33684923 MsaT002191.1:CDS
TCACTACAGAAGGAATGCTA+GGG 0.640516 1:-33686070 MsaT002191.1:CDS
AGAGGAGGAGTTCCAATGGG+AGG 0.644588 1:+33686101 None:intergenic
AAGGTTTCTCCTGATCCAGT+AGG 0.649090 1:-33686497 MsaT002191.1:intron
AATCTGTAGCATGCTCGGCA+GGG 0.649746 1:+33681480 None:intergenic
TAGCACAATCACCAGTGACA+GGG 0.654726 1:-33673009 MsaT002191.1:CDS
GTAGTGATAGCATTTAGAGG+AGG 0.657190 1:+33686086 None:intergenic
ACTGGTACAGGAAATACACT+TGG 0.661183 1:-33681925 MsaT002191.1:CDS
AAGTACCTCTAAATATGCTG+CGG 0.661658 1:+33686286 None:intergenic
ACAATCACCAGTGACAGGGA+TGG 0.671107 1:-33673005 MsaT002191.1:CDS
ACCAGTTGCTGTGAATGCAA+AGG 0.674838 1:+33682644 None:intergenic
TTCATGAATGCAATCACCAG+AGG 0.679527 1:+33673657 None:intergenic
TGGCTCCTTAGATGATAATG+TGG 0.680795 1:-33681564 MsaT002191.1:CDS
AGGGAGGATCTACACAGGTG+AGG 0.687544 1:-33673983 MsaT002191.1:CDS
CATGCAGAGATCCTTACCTG+TGG 0.696101 1:-33685612 MsaT002191.1:CDS
TATGGTACTGAAGGAGCAGG+CGG 0.702719 1:-33681793 MsaT002191.1:CDS
ATGACCATACCAGCTCATGA+GGG 0.705801 1:-33673046 MsaT002191.1:CDS
CAAGCAGGGAGGATCTACAC+AGG 0.707367 1:-33673988 MsaT002191.1:intron
CTTCGTTTGATACAACTGTG+CGG 0.708138 1:-33677112 MsaT002191.1:CDS
TTTCTAGGGACACTCTGGGG+AGG 0.715436 1:-33673758 MsaT002191.1:intron
GGTATCCTCCGATAACCAAG+AGG 0.726567 1:+33673578 None:intergenic
GCAGCAATTGATGCTCCTGG+TGG 0.739437 1:-33686383 MsaT002191.1:intron
GTATTTCTAGGGACACTCTG+GGG 0.781503 1:-33673761 MsaT002191.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 33672961 33686723 33672961 ID=MsaG002191
Chr1 mRNA 33672961 33686723 33672961 ID=MsaT002191.1;Parent=MsaG002191
Chr1 exon 33672961 33673104 33672961 ID=MsaT002191.1.exon17;Parent=MsaT002191.1
Chr1 CDS 33672961 33673104 33672961 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33673576 33673773 33673576 ID=MsaT002191.1.exon16;Parent=MsaT002191.1
Chr1 CDS 33673576 33673773 33673576 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33673887 33674003 33673887 ID=MsaT002191.1.exon15;Parent=MsaT002191.1
Chr1 CDS 33673887 33674003 33673887 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33674089 33674244 33674089 ID=MsaT002191.1.exon14;Parent=MsaT002191.1
Chr1 CDS 33674089 33674244 33674089 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33677094 33677195 33677094 ID=MsaT002191.1.exon13;Parent=MsaT002191.1
Chr1 CDS 33677094 33677195 33677094 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33681294 33681403 33681294 ID=MsaT002191.1.exon12;Parent=MsaT002191.1
Chr1 CDS 33681294 33681403 33681294 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33681471 33681618 33681471 ID=MsaT002191.1.exon11;Parent=MsaT002191.1
Chr1 CDS 33681471 33681618 33681471 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33681768 33681995 33681768 ID=MsaT002191.1.exon10;Parent=MsaT002191.1
Chr1 CDS 33681768 33681995 33681768 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33682640 33682714 33682640 ID=MsaT002191.1.exon9;Parent=MsaT002191.1
Chr1 CDS 33682640 33682714 33682640 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33684886 33685158 33684886 ID=MsaT002191.1.exon8;Parent=MsaT002191.1
Chr1 CDS 33684886 33685158 33684886 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33685233 33685282 33685233 ID=MsaT002191.1.exon7;Parent=MsaT002191.1
Chr1 CDS 33685233 33685282 33685233 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33685555 33685663 33685555 ID=MsaT002191.1.exon6;Parent=MsaT002191.1
Chr1 CDS 33685555 33685663 33685555 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33685773 33685848 33685773 ID=MsaT002191.1.exon5;Parent=MsaT002191.1
Chr1 CDS 33685773 33685848 33685773 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33685933 33686216 33685933 ID=MsaT002191.1.exon4;Parent=MsaT002191.1
Chr1 CDS 33685933 33686216 33685933 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33686292 33686401 33686292 ID=MsaT002191.1.exon3;Parent=MsaT002191.1
Chr1 CDS 33686292 33686401 33686292 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33686498 33686595 33686498 ID=MsaT002191.1.exon2;Parent=MsaT002191.1
Chr1 CDS 33686498 33686595 33686498 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Chr1 exon 33686692 33686723 33686692 ID=MsaT002191.1.exon1;Parent=MsaT002191.1
Chr1 CDS 33686692 33686723 33686692 ID=cds.MsaT002191.1;Parent=MsaT002191.1
Gene Sequence

>MsaT002191.1

ATGGCGCAGAGTAATTGGGAAGCAGATAAGATGCTTGATGTTTACATATATGATTATTTGGTTAAGAAAAAGTTGCACAATACTGCTAAGTCATTTATGACAGAAGGGAAGGTTTCTCCTGATCCAGTAGCAATTGATGCTCCTGGTGGTTTTCTTTTCGAGTGGTGGTCTGTTTTCTGGGACATTTTTATCGCGAGGACAAATGAGAAACATTCTGATAATGCCGCAGCATATTTAGAGGCTCAACAGCTTAAAGCGAAAGAACAACAATTGCAAATGCAACAGTTGCAATTAATGCGCCAAGCTCAGATGCAAAGAAGGGATCCTAATCATCCTCCCATTGGAACTCCTCCTCTAAATGCTATCACTACAGAAGGAATGCTAGGGCAATCTACTGCCACTGCTTTGGCTGCAAAAATGTATGAGGATCGGATGAAAAACTCTAACCCTATGGATACAGAGACATCATCCCAACCACTTTTAGATGCTAGAATGGCCCTTTTGAAATCAACAAATCATCCTGGTCAGATGGTTCAAGGAAATTCAGTAAGTGTGACAGCAGCTTTGCAGCAAATGCAGGCTCGAACTCAGCAAACCCCTGATATCAAAAGCGAAGTCAACATGGGAAACATGCAGAGATCCTTACCTGTGGATCCTTCAATTTATGGGCAGGGAGGATTGCAGTCAAAGTCTGGAATCACAAATGCAGGATTAAATCAGGGAGTTGGTGGTCTCACATTGAAAGGATGGCCTTTAACTGGCATAGAACAAATTCGCCCAGGTTTTGGAGCTCAAGTTCAAAAGCCTCTCCTTCAAAGTGCAAATCAGTTTCAGCTTTTGCCACAACAACAACAACAACAGCTCCTTGCTCAGGTACATGCACAAGGAAATATTGGCAATTCACAAGTGTATGGAGATATGGATCCTCAACGATTAAGAGGATTAGCTAGAGGAGGTTTGAATGTGAAAGACAGTCAACCAATTGCAAACGATGGGTCAATAGGTTCTCCAATGCAATCTACTTCATCTAAGATCAACATGCCACAGATGCAACAATCCATCTCCCAGCAACAACATGATCCTTTGCATTCACAGCAACTGGTTCAGAATAACCGAAAAAGGAAAGGGCCTACATCTTCAGGAGCTGCCAATAGTACTGGTACAGGAAATACACTTGGCCCTTCTAATTCTCAACCGTCAACTCCATCTATTCATACTCCTGGTGATGGAGTTGCTATGGCGGGCAACTTGCAGAATGTTGCTGGTGTATCCAAGGGTTTGATGATTTATGGTACTGAAGGAGCAGGCGGTCTTGCATCTTCCACAAACCAGCTGTTGCAGGATGGCTTGGAACATTTTGGAGATGTTGGCTCCTTAGATGATAATGTGGAATCATTTCTCTCACAAGATGATGGTGATGGAAAGGACTTATTTGGGACGTTGAAACGGAACCCTGCCGAGCATGCTACAGATTCTTCAAAAGGCTTTTCCTTCAGTGAAGTTAGTTCTATACGCAAAAGCAATGGAAAAGTTGTTTGCTGTCATTTCTCTTCAGATGGGAAATTATTAGCCAGTGCTGGACATGACAAGAAGGAACACACTGTTATTATTACCGACGTTCGCTTCAGACCAAATTCAACTCAGCTGGCAACATCTTCGTTTGATACAACTGTGCGGCTTTGGGATGCTGCTGATCCAAGCCTTTCTTTGCAGGCATATAGCGGTCATACTTCACATGTTGCATCCCTTGATTTCCACCCTAAGAAAAATGACCTTTTCTGTTCCTGTGATGATAACAATGAGATTCGCTTCTGGAATATTAGTCAATATTCTTGTACTCGTGTTTTTAAGGGAGGATCTACACAGGTGAGGTTTCAGCCAAGGAGTGGACACCTTCTGGCCGCAGCATCTGGCAATGTTGTGTCTCTCTTTGATGTTGAGACTGACAGGCAAATGCACTCATTACAGGGACACTCTGGGGAGGTACATTGTGTATGCTGGGATACAAATGGAGATTATTTGGCATCTGTGAGTCAAGAATCTGTCAAAGTTTGGTCACTTGCCTCTGGTGATTGCATTCATGAACTTAATTCCAGTGGAAACATGTTTCATTCTTGTGTTTTTCACCCAAGTTACTCCAACCTCTTGGTTATCGGAGGATACCAGTCATTGGAGCTATGGCACATGGCCGAAAACAAATGTATGACCATACCAGCTCATGAGGGTGTAATATCAGCCCTAGCACAATCACCAGTGACAGGGATGGTTGCTTCTGCCAGTCATGATAAGTCGGTAAAGATATGGAAATAA
Protein sequence

>MsaT002191.1

MAQSNWEADKMLDVYIYDYLVKKKLHNTAKSFMTEGKVSPDPVAIDAPGGFLFEWWSVFWDIFIARTNEKHSDNAAAYLEAQQLKAKEQQLQMQQLQLMRQAQMQRRDPNHPPIGTPPLNAITTEGMLGQSTATALAAKMYEDRMKNSNPMDTETSSQPLLDARMALLKSTNHPGQMVQGNSVSVTAALQQMQARTQQTPDIKSEVNMGNMQRSLPVDPSIYGQGGLQSKSGITNAGLNQGVGGLTLKGWPLTGIEQIRPGFGAQVQKPLLQSANQFQLLPQQQQQQLLAQVHAQGNIGNSQVYGDMDPQRLRGLARGGLNVKDSQPIANDGSIGSPMQSTSSKINMPQMQQSISQQQHDPLHSQQLVQNNRKRKGPTSSGAANSTGTGNTLGPSNSQPSTPSIHTPGDGVAMAGNLQNVAGVSKGLMIYGTEGAGGLASSTNQLLQDGLEHFGDVGSLDDNVESFLSQDDGDGKDLFGTLKRNPAEHATDSSKGFSFSEVSSIRKSNGKVVCCHFSSDGKLLASAGHDKKEHTVIITDVRFRPNSTQLATSSFDTTVRLWDAADPSLSLQAYSGHTSHVASLDFHPKKNDLFCSCDDNNEIRFWNISQYSCTRVFKGGSTQVRFQPRSGHLLAAASGNVVSLFDVETDRQMHSLQGHSGEVHCVCWDTNGDYLASVSQESVKVWSLASGDCIHELNSSGNMFHSCVFHPSYSNLLVIGGYQSLELWHMAENKCMTIPAHEGVISALAQSPVTGMVASASHDKSVKIWK*