AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002285

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002285 MtrunA17_Chr1g0172861 80.000 245 36 3 1 233 32 275 9.93e-134 377
MsaG002285 MtrunA17_Chr1g0169561 37.329 292 109 12 1 236 40 313 1.68e-34 125
MsaG002285 MtrunA17_Chr1g0172961 40.323 248 83 11 1 221 32 241 1.19e-33 122
MsaG002285 MtrunA17_Chr1g0169521 34.932 292 116 11 1 236 40 313 1.28e-29 112
MsaG002285 MtrunA17_Chr4g0037041 52.252 111 36 3 1 97 32 139 1.72e-27 103
MsaG002285 MtrunA17_Chr1g0174871 33.333 264 135 9 1 236 32 282 1.25e-18 82.4
MsaG002285 MtrunA17_Chr7g0214511 35.600 250 101 15 11 236 71 284 4.21e-15 72.8
MsaG002285 MtrunA17_Chr1g0193731 33.333 213 71 9 35 233 62 217 4.25e-14 70.1
MsaG002285 MtrunA17_Chr1g0174861 32.653 147 82 4 20 149 40 186 6.14e-13 66.2
MsaG002285 MtrunA17_Chr1g0174001 35.644 202 91 11 56 236 9 192 5.80e-12 62.8
MsaG002285 MtrunA17_Chr1g0207451 45.070 71 34 2 2 67 83 153 6.59e-12 63.5
MsaG002285 MtrunA17_Chr2g0331601 30.872 149 73 5 2 120 138 286 7.00e-11 61.2
MsaG002285 MtrunA17_Chr1g0182161 38.636 88 42 3 2 83 39 120 7.42e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002285 AT3G19580.1 40.244 82 33 2 2 67 106 187 6.20e-12 64.3
MsaG002285 AT3G19580.2 40.244 82 33 2 2 67 106 187 6.20e-12 64.3
MsaG002285 AT3G49930.1 46.835 79 28 3 2 67 94 171 9.39e-12 62.8
MsaG002285 AT5G43170.1 44.286 70 39 0 2 71 75 144 2.09e-11 61.6
MsaG002285 AT2G37430.1 42.254 71 37 1 2 68 47 117 3.29e-11 60.8
MsaG002285 AT3G53600.1 41.176 68 39 1 2 68 49 116 3.94e-11 60.5
MsaG002285 AT1G27730.1 39.535 86 39 1 2 74 80 165 6.92e-11 60.5

Find 44 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCTCTTGCTACCTTTAT+TGG 0.117903 1:-36532565 None:intergenic
TTGACTTCAATTCATAATTA+TGG 0.207039 1:-36532911 None:intergenic
ACACAATCATGATGTTAATA+AGG 0.285362 1:+36532383 MsaT002285.1:CDS
TCAAGCAATTCTGAAGATAT+TGG 0.296091 1:+36532801 MsaT002285.1:CDS
GAAACTTTCCTGCGGTGATA+TGG 0.327630 1:+36532662 MsaT002285.1:CDS
GTGGCCACCGAGTGCGTTGC+CGG 0.328183 1:-36532319 None:intergenic
AAAGATGACAGCGGAAATAA+AGG 0.333169 1:+36532768 MsaT002285.1:CDS
TTATTGGTCTGATGCGATAT+TGG 0.368016 1:-36532549 None:intergenic
CTTCAGAATTGCTTGAAAGT+TGG 0.379821 1:-36532794 None:intergenic
ATATCGCATCAGACCAATAA+AGG 0.383036 1:+36532552 MsaT002285.1:CDS
TGCTAACTGCGAGTCATGTT+AGG 0.385892 1:+36532715 MsaT002285.1:CDS
ATATCCTTTACCTTGGTCTC+TGG 0.394011 1:-36532840 None:intergenic
CTCTTCAATATCCTTTACCT+TGG 0.428483 1:-36532847 None:intergenic
TTCAATTCATAATTATGGCT+TGG 0.451006 1:-36532906 None:intergenic
CCACACAAAGCCTGCTTCGA+TGG 0.481719 1:-36532444 None:intergenic
ATATCTCATGTTGATAATCA+TGG 0.491426 1:+36532624 MsaT002285.1:CDS
TGATTTGAATGAGCTTCTGA+TGG 0.509964 1:+36532953 MsaT002285.1:CDS
ATGAGATGTCACAAAAGAGA+TGG 0.516344 1:+36532471 MsaT002285.1:CDS
ATAATCATGGTGACAGTAAG+AGG 0.520851 1:+36532637 MsaT002285.1:CDS
TTCAACTCCGGCAACGCACT+CGG 0.530443 1:+36532312 MsaT002285.1:CDS
GCTAACTGCGAGTCATGTTA+GGG 0.531070 1:+36532716 MsaT002285.1:CDS
GAGAAGAAGAACAATAATCA+AGG 0.543568 1:+36532867 MsaT002285.1:CDS
CAATAGATGATGAAGTTGGC+AGG 0.546568 1:-36532506 None:intergenic
CCATCGAAGCAGGCTTTGTG+TGG 0.551200 1:+36532444 MsaT002285.1:CDS
ATCACCAGAGACCAAGGTAA+AGG 0.555674 1:+36532836 MsaT002285.1:CDS
CTCATTAACCATATCACCGC+AGG 0.561714 1:-36532670 None:intergenic
TGAGATGTCACAAAAGAGAT+GGG 0.575280 1:+36532472 MsaT002285.1:CDS
AGTGTGAGTTGTGCGGCAAA+AGG 0.579769 1:+36532289 MsaT002285.1:CDS
CGGTGATATGGTTAATGAGA+CGG 0.580945 1:+36532674 MsaT002285.1:CDS
ATTATGGCTTGGTTCCCTCA+CGG 0.584200 1:-36532895 None:intergenic
TAGTTGTCATGTTTGCAACA+AGG 0.593032 1:+36532416 MsaT002285.1:CDS
AAGTCAATAGATGATGAAGT+TGG 0.601075 1:-36532510 None:intergenic
TGCGGCAAAAGGTTCAACTC+CGG 0.613826 1:+36532300 MsaT002285.1:CDS
AGACCAATAAAGGTAGCAAG+AGG 0.628089 1:+36532562 MsaT002285.1:CDS
TATGGTTAATGAGACGGTCA+AGG 0.630287 1:+36532680 MsaT002285.1:CDS
ATAATCAAGGTGAAACCGTG+AGG 0.659754 1:+36532880 MsaT002285.1:CDS
TAATCAAGGTGAAACCGTGA+GGG 0.660486 1:+36532881 MsaT002285.1:CDS
TGATGAATCACCAGAGACCA+AGG 0.664340 1:+36532830 MsaT002285.1:CDS
AACTCCGGCAACGCACTCGG+TGG 0.667301 1:+36532315 MsaT002285.1:CDS
ATCATGGTGACAGTAAGAGG+CGG 0.693631 1:+36532640 MsaT002285.1:CDS
ATGTATGAGTGTGAGTTGTG+CGG 0.703631 1:+36532282 MsaT002285.1:CDS
TAGGGTTAATGATGAAACAG+TGG 0.707642 1:+36532734 MsaT002285.1:CDS
ATCAAAGAGAAAGATGACAG+CGG 0.723705 1:+36532759 MsaT002285.1:CDS
AAGAGGCGGAAACTTTCCTG+CGG 0.732867 1:+36532654 MsaT002285.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 36532282 36532992 36532282 ID=MsaG002285
Chr1 mRNA 36532282 36532992 36532282 ID=MsaT002285.1;Parent=MsaG002285
Chr1 exon 36532282 36532992 36532282 ID=MsaT002285.1.exon1;Parent=MsaT002285.1
Chr1 CDS 36532282 36532992 36532282 ID=cds.MsaT002285.1;Parent=MsaT002285.1
Gene Sequence

>MsaT002285.1

ATGTATGAGTGTGAGTTGTGCGGCAAAAGGTTCAACTCCGGCAACGCACTCGGTGGCCACAAAACATCTCACCGCTCACAACCTCAAAACAAGAAACAAAAACACAATCATGATGTTAATAAGGATGACAAACATAGTTGTCATGTTTGCAACAAGGTTTTTCCATCGAAGCAGGCTTTGTGTGGACACATGAGATGTCACAAAAGAGATGGGAAGAATATTCACCTGCCAACTTCATCATCTATTGACTTATCAAAATATATGCTACCAATATCGCATCAGACCAATAAAGGTAGCAAGAGGAATATCATTGATGAAGATGTTGCTCGAACAATCATGCAAATATCTCATGTTGATAATCATGGTGACAGTAAGAGGCGGAAACTTTCCTGCGGTGATATGGTTAATGAGACGGTCAAGGAACAAACTGTGATGCTAACTGCGAGTCATGTTAGGGTTAATGATGAAACAGTGGTGATCAAAGAGAAAGATGACAGCGGAAATAAAGGACTCCAACTTTCAAGCAATTCTGAAGATATTGGTGACGTTGATGAATCACCAGAGACCAAGGTAAAGGATATTGAAGAGAAGAAGAACAATAATCAAGGTGAAACCGTGAGGGAACCAAGCCATAATTATGAATTGAAGTCAAATAGAGTTGTTATGAATTCTGATTTGAATGAGCTTCTGATGGAAGATGTTGCAGATTAG
Protein sequence

>MsaT002285.1

MYECELCGKRFNSGNALGGHKTSHRSQPQNKKQKHNHDVNKDDKHSCHVCNKVFPSKQALCGHMRCHKRDGKNIHLPTSSSIDLSKYMLPISHQTNKGSKRNIIDEDVARTIMQISHVDNHGDSKRRKLSCGDMVNETVKEQTVMLTASHVRVNDETVVIKEKDDSGNKGLQLSSNSEDIGDVDESPETKVKDIEEKKNNNQGETVREPSHNYELKSNRVVMNSDLNELLMEDVAD*