Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002403 | RHN78770.1 | 69.632 | 326 | 62 | 3 | 1 | 299 | 1 | 316 | 2.71e-138 | 403 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002403 | tr|A0A396JKH3|A0A396JKH3_MEDTR | 69.632 | 326 | 62 | 3 | 1 | 299 | 1 | 316 | 1.29e-138 | 403 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG002403 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000613 | MsaG002403 | 0.800968 | 1.881426e-48 | 2.599930e-46 |
| MsaG002403 | MsaG002885 | 0.803036 | 7.101716e-49 | 1.028781e-46 |
| MsaG002403 | MsaG003335 | 0.802289 | 1.010933e-48 | 1.439620e-46 |
| MsaG002403 | MsaG007821 | 0.818280 | 3.711738e-52 | 7.803300e-50 |
| MsaG002403 | MsaG010644 | 0.801089 | 1.777260e-48 | 2.462721e-46 |
| MsaG002403 | MsaG013264 | 0.827381 | 2.880961e-54 | 7.742864e-52 |
| MsaG002403 | MsaG017136 | 0.804298 | 3.897296e-49 | 5.813306e-47 |
| MsaG002403 | MsaG028844 | 0.805777 | 1.917739e-49 | 2.960490e-47 |
| MsaG002403 | MsaG030519 | 0.824810 | 1.169613e-53 | 2.926910e-51 |
| MsaG002403 | MsaG031352 | 0.820447 | 1.196561e-52 | 2.663127e-50 |
| MsaG002403 | MsaG032180 | 0.815671 | 1.421692e-51 | 2.795340e-49 |
| MsaG002403 | MsaG035391 | 0.803836 | 4.858163e-49 | 7.168995e-47 |
| MsaG002403 | MsaG043850 | 0.813707 | 3.852332e-51 | 7.208704e-49 |
| MsaG002403 | MsaG044105 | 0.806166 | 1.590367e-49 | 2.477751e-47 |
| MsaG002403 | MsaG044571 | 0.832249 | 1.903435e-55 | 5.878410e-53 |
| MsaG002403 | MsaG019915 | 0.805939 | 1.774026e-49 | 2.749115e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002403 | MtrunA17_Chr1g0169561 | 69.632 | 326 | 62 | 3 | 1 | 299 | 1 | 316 | 2.49e-142 | 403 |
| MsaG002403 | MtrunA17_Chr1g0169521 | 68.098 | 326 | 67 | 3 | 1 | 299 | 1 | 316 | 5.47e-136 | 387 |
| MsaG002403 | MtrunA17_Chr1g0174001 | 88.718 | 195 | 19 | 1 | 108 | 299 | 1 | 195 | 9.01e-122 | 347 |
| MsaG002403 | MtrunA17_Chr7g0214511 | 54.065 | 246 | 75 | 9 | 67 | 299 | 67 | 287 | 4.42e-63 | 201 |
| MsaG002403 | MtrunA17_Chr1g0174861 | 38.776 | 245 | 103 | 11 | 36 | 276 | 38 | 239 | 7.98e-33 | 121 |
| MsaG002403 | MtrunA17_Chr1g0193731 | 39.419 | 241 | 68 | 9 | 67 | 298 | 51 | 222 | 3.21e-29 | 112 |
| MsaG002403 | MtrunA17_Chr1g0174871 | 39.910 | 223 | 101 | 11 | 90 | 298 | 81 | 284 | 4.54e-28 | 109 |
| MsaG002403 | MtrunA17_Chr1g0172861 | 34.835 | 333 | 113 | 17 | 5 | 299 | 12 | 278 | 1.40e-22 | 94.7 |
| MsaG002403 | MtrunA17_Chr1g0172961 | 32.770 | 296 | 139 | 13 | 5 | 282 | 12 | 265 | 6.23e-21 | 90.1 |
| MsaG002403 | MtrunA17_Chr1g0180581 | 33.906 | 233 | 85 | 10 | 67 | 299 | 57 | 220 | 3.36e-18 | 81.6 |
| MsaG002403 | MtrunA17_Chr4g0037041 | 52.273 | 88 | 32 | 5 | 74 | 157 | 58 | 139 | 8.92e-14 | 68.6 |
| MsaG002403 | MtrunA17_Chr1g0180571 | 50.667 | 75 | 21 | 2 | 219 | 293 | 13 | 71 | 1.78e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAAAAGAGAGACACTAAT+TGG | 0.250890 | 1:-40214802 | None:intergenic |
| CGAATCACTGTCATAGTAAA+AGG | 0.272104 | 1:+40214496 | MsaT002403.1:CDS |
| AGCGAAGGTGAAACTGAATT+AGG | 0.276698 | 1:+40214717 | MsaT002403.1:CDS |
| GCAAAAGATTTCCAGTAATA+TGG | 0.318067 | 1:+40214518 | MsaT002403.1:CDS |
| GCTATCATCCACCATATTAC+TGG | 0.352563 | 1:-40214529 | None:intergenic |
| CTCAAGATGGCTCTGTTTCA+AGG | 0.407872 | 1:-40214298 | None:intergenic |
| GTGATTCGCATCAAGATTAC+CGG | 0.407962 | 1:-40214481 | None:intergenic |
| CACAATCATAATCATGATGA+TGG | 0.409442 | 1:+40214138 | MsaT002403.1:CDS |
| GCGAAGGTGAAACTGAATTA+GGG | 0.418343 | 1:+40214718 | MsaT002403.1:CDS |
| TTTAGGTTTAAGAGGAATGT+TGG | 0.422630 | 1:-40214028 | None:intergenic |
| TCAATAAAATGTCTTCTCCT+TGG | 0.432037 | 1:-40214387 | None:intergenic |
| ATGTCCGTTCAAAGCATTGT+TGG | 0.440760 | 1:-40214208 | None:intergenic |
| CGTTATTCACAGCATGATCA+TGG | 0.444828 | 1:-40214065 | None:intergenic |
| GACTCTAACCGTGATATTGA+AGG | 0.464706 | 1:+40214681 | MsaT002403.1:CDS |
| GGCTCTGTTTCAAGGAAGAT+CGG | 0.474884 | 1:-40214290 | None:intergenic |
| AATGTTGGAGGAGTGGTGAC+TGG | 0.475721 | 1:-40214013 | None:intergenic |
| ATATCACGGTTAGAGTCTAT+AGG | 0.475889 | 1:-40214675 | None:intergenic |
| ACTCAAAAGGGTTCGAAGAA+TGG | 0.483594 | 1:+40214243 | MsaT002403.1:CDS |
| TATGAGATGGCATACTCAAA+AGG | 0.486406 | 1:+40214230 | MsaT002403.1:CDS |
| TTGCCGCAACGAAAAGAACT+TGG | 0.515791 | 1:+40214593 | MsaT002403.1:CDS |
| TTAGTCTTGTGAGATATTGG+TGG | 0.516257 | 1:-40214354 | None:intergenic |
| AACAAGGTGTGCTGCTAACA+AGG | 0.519240 | 1:+40214565 | MsaT002403.1:CDS |
| TACTTTGACAAGTCATTAGC+CGG | 0.522449 | 1:-40214327 | None:intergenic |
| ATGAGATGGCATACTCAAAA+GGG | 0.526510 | 1:+40214231 | MsaT002403.1:CDS |
| GCTCTGTTTCAAGGAAGATC+GGG | 0.528874 | 1:-40214289 | None:intergenic |
| CATGATCATGCTGTGAATAA+CGG | 0.530498 | 1:+40214066 | MsaT002403.1:CDS |
| AGGTTTAAGAGGAATGTTGG+AGG | 0.532088 | 1:-40214025 | None:intergenic |
| TCATCTTCAAAATTGTCACT+TGG | 0.534630 | 1:-40214777 | None:intergenic |
| TCATCCAACAATGCTTTGAA+CGG | 0.535361 | 1:+40214204 | MsaT002403.1:CDS |
| AAGATCGGGCAGTCGGTGGG+TGG | 0.537715 | 1:-40214275 | None:intergenic |
| AAGGAAGATCGGGCAGTCGG+TGG | 0.543693 | 1:-40214279 | None:intergenic |
| TCAAACGAATGGTGAGAAGA+AGG | 0.549246 | 1:-40213931 | None:intergenic |
| AGGAAGATCGGGCAGTCGGT+GGG | 0.550277 | 1:-40214278 | None:intergenic |
| TAAGAGGAATGTTGGAGGAG+TGG | 0.552193 | 1:-40214020 | None:intergenic |
| TCTTCTTGCCTTCAATATCA+CGG | 0.552226 | 1:-40214689 | None:intergenic |
| TACATGTGACGTTTGTAACA+AGG | 0.552611 | 1:+40214176 | MsaT002403.1:CDS |
| TGTTACAAACGTCACATGTA+CGG | 0.556278 | 1:-40214173 | None:intergenic |
| ACAATCATAATCATGATGAT+GGG | 0.567903 | 1:+40214139 | MsaT002403.1:CDS |
| GGATGATAGCGAGAAGATGA+AGG | 0.572753 | 1:+40214542 | MsaT002403.1:CDS |
| CACTGTTGTATATATCTCGA+GGG | 0.580481 | 1:+40214442 | MsaT002403.1:CDS |
| GAGCCATCTTGAGTTTCCAC+CGG | 0.591664 | 1:+40214308 | MsaT002403.1:CDS |
| AAAGATTTCCAGTAATATGG+TGG | 0.595472 | 1:+40214521 | MsaT002403.1:CDS |
| GGCAAGAAGAAGATTAGCGA+AGG | 0.606572 | 1:+40214702 | MsaT002403.1:CDS |
| TTCAAGGAAGATCGGGCAGT+CGG | 0.606633 | 1:-40214282 | None:intergenic |
| TCTGTTGGTGATCATGATCA+TGG | 0.608195 | 1:-40213969 | None:intergenic |
| CTTTGAACGGACATATGAGA+TGG | 0.613266 | 1:+40214217 | MsaT002403.1:CDS |
| ACGAAAAGAACTTGGTGTTG+AGG | 0.613989 | 1:+40214601 | MsaT002403.1:CDS |
| ACACTGTTGTATATATCTCG+AGG | 0.619081 | 1:+40214441 | MsaT002403.1:CDS |
| TGATTCGCATCAAGATTACC+GGG | 0.632927 | 1:-40214480 | None:intergenic |
| TAGCCGGTGGAAACTCAAGA+TGG | 0.650157 | 1:-40214311 | None:intergenic |
| ACTGTTGTATATATCTCGAG+GGG | 0.661339 | 1:+40214443 | MsaT002403.1:CDS |
| AGCGAGAAGATGAAGGAACA+AGG | 0.665288 | 1:+40214549 | MsaT002403.1:CDS |
| TTTGACAAGTCATTAGCCGG+TGG | 0.679594 | 1:-40214324 | None:intergenic |
| CTGTTGTATATATCTCGAGG+GGG | 0.727133 | 1:+40214444 | MsaT002403.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 40213910 | 40214809 | 40213910 | ID=MsaG002403 |
| Chr1 | mRNA | 40213910 | 40214809 | 40213910 | ID=MsaT002403.1;Parent=MsaG002403 |
| Chr1 | exon | 40213910 | 40214809 | 40213910 | ID=MsaT002403.1.exon1;Parent=MsaT002403.1 |
| Chr1 | CDS | 40213910 | 40214809 | 40213910 | ID=cds.MsaT002403.1;Parent=MsaT002403.1 |
| Gene Sequence |
| Protein sequence |