AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002403


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002403 MtrunA17_Chr1g0169561 69.632 326 62 3 1 299 1 316 2.49e-142 403
MsaG002403 MtrunA17_Chr1g0169521 68.098 326 67 3 1 299 1 316 5.47e-136 387
MsaG002403 MtrunA17_Chr1g0174001 88.718 195 19 1 108 299 1 195 9.01e-122 347
MsaG002403 MtrunA17_Chr7g0214511 54.065 246 75 9 67 299 67 287 4.42e-63 201
MsaG002403 MtrunA17_Chr1g0174861 38.776 245 103 11 36 276 38 239 7.98e-33 121
MsaG002403 MtrunA17_Chr1g0193731 39.419 241 68 9 67 298 51 222 3.21e-29 112
MsaG002403 MtrunA17_Chr1g0174871 39.910 223 101 11 90 298 81 284 4.54e-28 109
MsaG002403 MtrunA17_Chr1g0172861 34.835 333 113 17 5 299 12 278 1.40e-22 94.7
MsaG002403 MtrunA17_Chr1g0172961 32.770 296 139 13 5 282 12 265 6.23e-21 90.1
MsaG002403 MtrunA17_Chr1g0180581 33.906 233 85 10 67 299 57 220 3.36e-18 81.6
MsaG002403 MtrunA17_Chr4g0037041 52.273 88 32 5 74 157 58 139 8.92e-14 68.6
MsaG002403 MtrunA17_Chr1g0180571 50.667 75 21 2 219 293 13 71 1.78e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAAAAGAGAGACACTAAT+TGG 0.250890 1:-40214802 None:intergenic
CGAATCACTGTCATAGTAAA+AGG 0.272104 1:+40214496 MsaT002403.1:CDS
AGCGAAGGTGAAACTGAATT+AGG 0.276698 1:+40214717 MsaT002403.1:CDS
GCAAAAGATTTCCAGTAATA+TGG 0.318067 1:+40214518 MsaT002403.1:CDS
GCTATCATCCACCATATTAC+TGG 0.352563 1:-40214529 None:intergenic
CTCAAGATGGCTCTGTTTCA+AGG 0.407872 1:-40214298 None:intergenic
GTGATTCGCATCAAGATTAC+CGG 0.407962 1:-40214481 None:intergenic
CACAATCATAATCATGATGA+TGG 0.409442 1:+40214138 MsaT002403.1:CDS
GCGAAGGTGAAACTGAATTA+GGG 0.418343 1:+40214718 MsaT002403.1:CDS
TTTAGGTTTAAGAGGAATGT+TGG 0.422630 1:-40214028 None:intergenic
TCAATAAAATGTCTTCTCCT+TGG 0.432037 1:-40214387 None:intergenic
ATGTCCGTTCAAAGCATTGT+TGG 0.440760 1:-40214208 None:intergenic
CGTTATTCACAGCATGATCA+TGG 0.444828 1:-40214065 None:intergenic
GACTCTAACCGTGATATTGA+AGG 0.464706 1:+40214681 MsaT002403.1:CDS
GGCTCTGTTTCAAGGAAGAT+CGG 0.474884 1:-40214290 None:intergenic
AATGTTGGAGGAGTGGTGAC+TGG 0.475721 1:-40214013 None:intergenic
ATATCACGGTTAGAGTCTAT+AGG 0.475889 1:-40214675 None:intergenic
ACTCAAAAGGGTTCGAAGAA+TGG 0.483594 1:+40214243 MsaT002403.1:CDS
TATGAGATGGCATACTCAAA+AGG 0.486406 1:+40214230 MsaT002403.1:CDS
TTGCCGCAACGAAAAGAACT+TGG 0.515791 1:+40214593 MsaT002403.1:CDS
TTAGTCTTGTGAGATATTGG+TGG 0.516257 1:-40214354 None:intergenic
AACAAGGTGTGCTGCTAACA+AGG 0.519240 1:+40214565 MsaT002403.1:CDS
TACTTTGACAAGTCATTAGC+CGG 0.522449 1:-40214327 None:intergenic
ATGAGATGGCATACTCAAAA+GGG 0.526510 1:+40214231 MsaT002403.1:CDS
GCTCTGTTTCAAGGAAGATC+GGG 0.528874 1:-40214289 None:intergenic
CATGATCATGCTGTGAATAA+CGG 0.530498 1:+40214066 MsaT002403.1:CDS
AGGTTTAAGAGGAATGTTGG+AGG 0.532088 1:-40214025 None:intergenic
TCATCTTCAAAATTGTCACT+TGG 0.534630 1:-40214777 None:intergenic
TCATCCAACAATGCTTTGAA+CGG 0.535361 1:+40214204 MsaT002403.1:CDS
AAGATCGGGCAGTCGGTGGG+TGG 0.537715 1:-40214275 None:intergenic
AAGGAAGATCGGGCAGTCGG+TGG 0.543693 1:-40214279 None:intergenic
TCAAACGAATGGTGAGAAGA+AGG 0.549246 1:-40213931 None:intergenic
AGGAAGATCGGGCAGTCGGT+GGG 0.550277 1:-40214278 None:intergenic
TAAGAGGAATGTTGGAGGAG+TGG 0.552193 1:-40214020 None:intergenic
TCTTCTTGCCTTCAATATCA+CGG 0.552226 1:-40214689 None:intergenic
TACATGTGACGTTTGTAACA+AGG 0.552611 1:+40214176 MsaT002403.1:CDS
TGTTACAAACGTCACATGTA+CGG 0.556278 1:-40214173 None:intergenic
ACAATCATAATCATGATGAT+GGG 0.567903 1:+40214139 MsaT002403.1:CDS
GGATGATAGCGAGAAGATGA+AGG 0.572753 1:+40214542 MsaT002403.1:CDS
CACTGTTGTATATATCTCGA+GGG 0.580481 1:+40214442 MsaT002403.1:CDS
GAGCCATCTTGAGTTTCCAC+CGG 0.591664 1:+40214308 MsaT002403.1:CDS
AAAGATTTCCAGTAATATGG+TGG 0.595472 1:+40214521 MsaT002403.1:CDS
GGCAAGAAGAAGATTAGCGA+AGG 0.606572 1:+40214702 MsaT002403.1:CDS
TTCAAGGAAGATCGGGCAGT+CGG 0.606633 1:-40214282 None:intergenic
TCTGTTGGTGATCATGATCA+TGG 0.608195 1:-40213969 None:intergenic
CTTTGAACGGACATATGAGA+TGG 0.613266 1:+40214217 MsaT002403.1:CDS
ACGAAAAGAACTTGGTGTTG+AGG 0.613989 1:+40214601 MsaT002403.1:CDS
ACACTGTTGTATATATCTCG+AGG 0.619081 1:+40214441 MsaT002403.1:CDS
TGATTCGCATCAAGATTACC+GGG 0.632927 1:-40214480 None:intergenic
TAGCCGGTGGAAACTCAAGA+TGG 0.650157 1:-40214311 None:intergenic
ACTGTTGTATATATCTCGAG+GGG 0.661339 1:+40214443 MsaT002403.1:CDS
AGCGAGAAGATGAAGGAACA+AGG 0.665288 1:+40214549 MsaT002403.1:CDS
TTTGACAAGTCATTAGCCGG+TGG 0.679594 1:-40214324 None:intergenic
CTGTTGTATATATCTCGAGG+GGG 0.727133 1:+40214444 MsaT002403.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 40213910 40214809 40213910 ID=MsaG002403
Chr1 mRNA 40213910 40214809 40213910 ID=MsaT002403.1;Parent=MsaG002403
Chr1 exon 40213910 40214809 40213910 ID=MsaT002403.1.exon1;Parent=MsaT002403.1
Chr1 CDS 40213910 40214809 40213910 ID=cds.MsaT002403.1;Parent=MsaT002403.1
Gene Sequence

>MsaT002403.1

ATGAACAAACACTACTCTTCACCTTCTTCTCACCATTCGTTTGATAAACCGAAAAACAGCCATGATCATGATCACCAACAGATTCTTGAAAATGATAAGAAAACCAGTCACCACTCCTCCAACATTCCTCTTAAACCTAAAACAAACAAACAGAGCCATGATCATGCTGTGAATAACGGAACGTTGAAGAAGACAGCAAAAATCACTTGGGTTTTTTCTTCTTCTCTTCACAATCATAATCATGATGATGGGAAACTGAAGAACCGTACATGTGACGTTTGTAACAAGGTTTTTTCATCCAACAATGCTTTGAACGGACATATGAGATGGCATACTCAAAAGGGTTCGAAGAATGGTGTTATTTTCCACCCACCGACTGCCCGATCTTCCTTGAAACAGAGCCATCTTGAGTTTCCACCGGCTAATGACTTGTCAAAGTACTTACCACCAATATCTCACAAGACTAAAAAGCGGAGTCCAAGGAGAAGACATTTTATTGATAATGATGAAGAACTTAATATTGCTGCAGAAACACTGTTGTATATATCTCGAGGGGGTTGTGAGGGATTGCCCGGTAATCTTGATGCGAATCACTGTCATAGTAAAAGGCAAAAGATTTCCAGTAATATGGTGGATGATAGCGAGAAGATGAAGGAACAAGGTGTGCTGCTAACAAGGTGTGTTTGCCGCAACGAAAAGAACTTGGTGTTGAGGTTGAAGATTCCTAAAGACAAGATTTTGCAAAAATCACAAGATTGCAAGGAACCTATAGACTCTAACCGTGATATTGAAGGCAAGAAGAAGATTAGCGAAGGTGAAACTGAATTAGGGTCAAGAGTTGTTATGAATTTTGATTTGAATGAGATTCCAAGTGACAATTTTGAAGATGAAACCAATTAG

Protein sequence

>MsaT002403.1

MNKHYSSPSSHHSFDKPKNSHDHDHQQILENDKKTSHHSSNIPLKPKTNKQSHDHAVNNGTLKKTAKITWVFSSSLHNHNHDDGKLKNRTCDVCNKVFSSNNALNGHMRWHTQKGSKNGVIFHPPTARSSLKQSHLEFPPANDLSKYLPPISHKTKKRSPRRRHFIDNDEELNIAAETLLYISRGGCEGLPGNLDANHCHSKRQKISSNMVDDSEKMKEQGVLLTRCVCRNEKNLVLRLKIPKDKILQKSQDCKEPIDSNRDIEGKKKISEGETELGSRVVMNFDLNEIPSDNFEDETN*