AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002409

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002409 MtrunA17_Chr1g0174161 97.771 314 7 0 1 314 1 314 0.0 632
MsaG002409 MtrunA17_Chr7g0258161 67.791 326 79 8 1 306 1 320 1.01e-129 381
MsaG002409 MtrunA17_Chr4g0035101 56.934 137 52 3 115 245 93 228 9.66e-40 147
MsaG002409 MtrunA17_Chr1g0152131 43.500 200 84 6 104 279 209 403 3.92e-38 145
MsaG002409 MtrunA17_Chr8g0385911 60.163 123 38 5 151 268 134 250 1.63e-37 140
MsaG002409 MtrunA17_Chr7g0234761 46.196 184 79 5 116 285 259 436 1.76e-37 145
MsaG002409 MtrunA17_Chr3g0144641 52.778 144 49 3 129 253 141 284 2.61e-37 142
MsaG002409 MtrunA17_Chr8g0365941 55.224 134 51 3 112 242 260 387 1.10e-36 141
MsaG002409 MtrunA17_Chr5g0421111 70.787 89 26 0 157 245 362 450 4.73e-36 141
MsaG002409 MtrunA17_Chr8g0339091 53.719 121 50 1 144 258 269 389 5.24e-35 137
MsaG002409 MtrunA17_Chr3g0131771 56.198 121 42 3 153 268 138 252 5.68e-35 134
MsaG002409 MtrunA17_Chr8g0363921 69.412 85 26 0 157 241 148 232 3.71e-34 130
MsaG002409 MtrunA17_Chr5g0415291 55.118 127 51 2 108 231 60 183 1.49e-33 127
MsaG002409 MtrunA17_Chr6g0484271 43.396 159 71 3 82 237 90 232 2.25e-29 117
MsaG002409 MtrunA17_Chr4g0074161 49.485 97 39 3 156 248 202 292 1.58e-17 84.7
MsaG002409 MtrunA17_Chr1g0190731 39.200 125 68 4 160 282 193 311 1.18e-16 81.6
MsaG002409 MtrunA17_Chr5g0441151 48.750 80 38 1 439 515 78 157 3.56e-16 80.5
MsaG002409 MtrunA17_Chr7g0271301 44.444 81 45 0 435 515 124 204 1.52e-15 77.8
MsaG002409 MtrunA17_Chr1g0171991 44.595 74 41 0 441 514 37 110 4.13e-15 77.8
MsaG002409 MtrunA17_Chr5g0439091 44.156 77 43 0 439 515 6 82 6.40e-15 71.2
MsaG002409 MtrunA17_Chr1g0178241 44.156 77 43 0 439 515 141 217 3.62e-14 75.5
MsaG002409 MtrunA17_Chr4g0014771 45.833 72 38 1 153 224 112 182 4.42e-12 65.1
MsaG002409 MtrunA17_Chr4g0032951 45.833 72 38 1 153 224 349 419 6.32e-12 67.8
MsaG002409 MtrunA17_Chr1g0182791 29.412 187 104 5 162 339 319 486 7.96e-12 67.8
MsaG002409 MtrunA17_Chr1g0184841 49.425 87 38 3 160 241 120 205 2.09e-11 64.7
MsaG002409 MtrunA17_Chr7g0235231 45.333 75 41 0 441 515 70 144 2.57e-11 65.5
MsaG002409 MtrunA17_Chr1g0193321 44.444 63 34 1 163 225 465 526 5.25e-11 65.5
MsaG002409 MtrunA17_Chr3g0095391 31.132 106 71 2 162 267 311 414 6.51e-11 64.7
MsaG002409 MtrunA17_Chr1g0174271 43.421 76 42 1 153 228 292 366 7.51e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002409 AT3G57800.2 49.591 367 128 13 2 320 19 376 5.13e-84 266
MsaG002409 AT2G42300.1 51.282 312 105 10 6 295 20 306 3.95e-78 249
MsaG002409 AT3G57800.1 43.961 414 128 14 2 320 19 423 7.73e-75 243
MsaG002409 AT2G42300.4 52.239 268 83 7 6 245 20 270 3.16e-68 221
MsaG002409 AT3G07340.2 39.858 281 112 8 71 320 138 392 5.26e-43 159
MsaG002409 AT3G07340.1 39.858 281 112 8 71 320 138 392 1.71e-42 158
MsaG002409 AT1G26260.4 37.647 255 104 8 68 268 77 330 1.12e-41 154
MsaG002409 AT1G26260.1 37.647 255 104 8 68 268 77 330 1.12e-41 154
MsaG002409 AT1G26260.6 37.647 255 104 8 68 268 77 330 1.12e-41 154
MsaG002409 AT1G26260.2 37.647 255 104 8 68 268 77 330 1.12e-41 154
MsaG002409 AT1G26260.3 49.032 155 69 3 121 266 124 277 2.63e-40 149
MsaG002409 AT2G42300.5 45.946 222 75 7 6 199 20 224 4.11e-40 145
MsaG002409 AT2G42300.3 45.946 222 75 7 6 199 20 224 4.11e-40 145
MsaG002409 AT2G42300.2 45.946 222 75 7 6 199 20 224 4.15e-40 145
MsaG002409 AT1G26260.5 48.101 158 72 3 120 268 152 308 4.61e-40 149
MsaG002409 AT3G23690.1 48.454 194 83 6 117 295 144 335 4.55e-39 146
MsaG002409 AT1G68920.1 60.684 117 40 2 157 268 300 415 7.34e-39 148
MsaG002409 AT1G68920.4 60.684 117 40 2 157 268 300 415 7.34e-39 148
MsaG002409 AT1G68920.2 60.684 117 40 2 157 268 300 415 7.34e-39 148
MsaG002409 AT1G68920.3 60.684 117 40 2 157 268 299 414 7.52e-39 148
MsaG002409 AT1G10120.2 51.748 143 61 1 113 255 167 301 4.07e-38 144
MsaG002409 AT1G10120.1 51.748 143 61 1 113 255 167 301 4.07e-38 144
MsaG002409 AT4G34530.1 56.800 125 47 2 157 281 169 286 1.21e-36 139
MsaG002409 AT5G50915.3 45.055 182 85 8 114 285 89 265 9.40e-36 134
MsaG002409 AT5G50915.4 45.055 182 85 8 114 285 89 265 9.40e-36 134
MsaG002409 AT5G48560.2 68.478 92 28 1 157 247 298 389 1.48e-35 139
MsaG002409 AT2G18300.1 43.636 165 68 3 122 267 130 288 2.28e-35 135
MsaG002409 AT5G48560.1 68.478 92 28 1 157 247 298 389 2.32e-35 139
MsaG002409 AT5G50915.1 48.171 164 70 8 114 267 89 247 2.58e-35 134
MsaG002409 AT5G50915.2 48.171 164 70 8 114 267 89 247 2.58e-35 134
MsaG002409 AT4G36540.2 47.482 139 66 2 115 246 89 227 4.55e-34 131
MsaG002409 AT4G36540.1 47.826 138 65 2 115 245 89 226 4.66e-34 131
MsaG002409 AT2G18300.2 46.715 137 56 1 122 241 130 266 5.68e-34 132
MsaG002409 AT1G59640.1 78.667 75 16 0 157 231 133 207 7.30e-34 129
MsaG002409 AT1G59640.2 65.979 97 33 0 157 253 133 229 1.01e-33 131
MsaG002409 AT2G18300.3 46.715 137 56 1 122 241 130 266 1.38e-33 131
MsaG002409 AT1G18400.2 68.750 80 25 0 158 237 143 222 1.72e-30 119
MsaG002409 AT1G18400.1 68.750 80 25 0 158 237 143 222 3.21e-30 119
MsaG002409 AT5G62610.2 72.000 75 21 0 157 231 150 224 3.58e-30 119
MsaG002409 AT5G62610.1 72.000 75 21 0 157 231 150 224 3.58e-30 119
MsaG002409 AT1G73830.2 68.354 79 25 0 159 237 146 224 8.56e-30 118
MsaG002409 AT1G73830.1 68.354 79 25 0 159 237 146 224 8.67e-30 118
MsaG002409 AT1G25330.1 62.637 91 30 1 151 237 91 181 3.47e-29 115
MsaG002409 AT2G24260.2 60.606 66 25 1 160 225 35 99 1.04e-16 80.5
MsaG002409 AT4G30980.1 62.121 66 24 1 160 225 130 194 1.38e-16 81.3
MsaG002409 AT4G30980.2 62.121 66 24 1 160 225 178 242 2.17e-16 81.3
MsaG002409 AT2G24260.1 60.606 66 25 1 160 225 138 202 3.24e-16 80.5
MsaG002409 AT5G58010.1 61.194 67 25 1 159 225 98 163 3.38e-16 79.7
MsaG002409 AT2G20180.7 39.286 112 53 3 117 225 172 271 2.31e-14 75.5
MsaG002409 AT2G20180.1 39.286 112 53 3 117 225 172 271 2.31e-14 75.5
MsaG002409 AT2G20180.5 39.286 112 53 3 117 225 172 271 2.31e-14 75.5
MsaG002409 AT2G20180.4 39.286 112 53 3 117 225 243 342 3.52e-14 75.5
MsaG002409 AT2G20180.6 39.286 112 53 3 117 225 243 342 3.52e-14 75.5
MsaG002409 AT2G20180.2 39.286 112 53 3 117 225 243 342 3.52e-14 75.5
MsaG002409 AT2G20180.3 39.286 112 53 3 117 225 243 342 3.52e-14 75.5
MsaG002409 AT2G20180.8 38.532 109 52 3 117 222 243 339 4.50e-13 70.9
MsaG002409 AT4G28800.1 42.466 73 41 1 163 235 252 323 6.04e-12 68.2
MsaG002409 AT4G28815.2 36.082 97 57 2 163 255 71 166 1.28e-11 65.1
MsaG002409 AT1G09530.3 46.032 63 33 1 163 225 340 401 2.44e-11 66.6
MsaG002409 AT1G09530.2 46.032 63 33 1 163 225 340 401 2.44e-11 66.6
MsaG002409 AT1G09530.5 46.032 63 33 1 163 225 340 401 2.44e-11 66.6
MsaG002409 AT1G09530.1 46.032 63 33 1 163 225 340 401 2.44e-11 66.6
MsaG002409 AT1G09530.4 46.032 63 33 1 163 225 340 401 2.44e-11 66.6
MsaG002409 AT1G09530.6 46.032 63 33 1 163 225 340 401 2.44e-11 66.6
MsaG002409 AT2G42280.1 38.889 90 54 1 134 223 254 342 5.76e-11 64.7
MsaG002409 AT4G28790.1 41.667 72 41 1 163 234 274 344 6.30e-11 65.1

Find 130 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCATTGTGTGGAATTTC+TGG 0.119338 1:+40417709 None:intergenic
TATTCTATGAAATGGGTTTC+TGG 0.135748 1:-40415641 MsaT002409.1:CDS
TCAAGGTTGAAATCAATTAT+TGG 0.224458 1:+40418551 None:intergenic
AAACTTATTATTCTATGAAA+TGG 0.268930 1:-40415649 MsaT002409.1:CDS
TGCATTCCACCAACCACTTT+GGG 0.284445 1:-40417653 MsaT002409.1:CDS
ATCAGGAACAGCATTGGTTT+TGG 0.289474 1:-40419205 MsaT002409.1:CDS
TTCTGGTCTTGAACCATGTT+TGG 0.291157 1:-40415624 MsaT002409.1:CDS
CAAGGTTGAAATCAATTATT+GGG 0.303962 1:+40418552 None:intergenic
CTTCACTCGTCGGAGATTAC+CGG 0.318302 1:-40424671 MsaT002409.1:CDS
AAATTTCCTTTACAGATATC+AGG 0.320893 1:-40419222 MsaT002409.1:intron
TATGACTCATCGGCAAATTC+AGG 0.337138 1:-40417577 MsaT002409.1:intron
ACAACTTTGTTTGAGATTGC+AGG 0.358176 1:-40414902 MsaT002409.1:CDS
TTTCTGGGGTCATGAAATTA+GGG 0.358922 1:+40417614 None:intergenic
AAAAGAGCACCCTTGTTATC+AGG 0.360366 1:+40415515 None:intergenic
CGGAAGCTCATCGCCGTTGC+CGG 0.381962 1:+40424260 None:intergenic
TTTCAACCTTGACAGCATAT+TGG 0.387389 1:-40418540 MsaT002409.1:CDS
TCCGGCGAAAACGGAGAATC+TGG 0.391498 1:+40424565 None:intergenic
AAAGATTGATGATACAAGTA+TGG 0.394847 1:+40415597 None:intergenic
ATGCATTCCACCAACCACTT+TGG 0.397314 1:-40417654 MsaT002409.1:CDS
AACTTATTATTCTATGAAAT+GGG 0.403765 1:-40415648 MsaT002409.1:CDS
ATCGCCGCCGACGAGAATTC+CGG 0.408348 1:-40424279 MsaT002409.1:CDS
TCAGAGTCGGTTGGAAAACC+TGG 0.413514 1:-40417091 MsaT002409.1:CDS
GGAAGGGAAAGTTAAATTAC+CGG 0.419559 1:+40424613 None:intergenic
TTGATAAGGCCACTAAGAAT+AGG 0.420130 1:-40414856 MsaT002409.1:CDS
CGAATTCCAAGAGTGCAAAA+AGG 0.420797 1:-40424445 MsaT002409.1:CDS
CGGTAAGTTCAGCGAGTTTG+TGG 0.421680 1:+40424535 None:intergenic
GAGTTCGCTGTTTCCGTCGC+CGG 0.429642 1:+40424740 None:intergenic
CAAGTCAGTGGATAGGGTTT+CGG 0.429991 1:+40424641 None:intergenic
ACTGAAAGTCCTATTCTTAG+TGG 0.443475 1:+40414847 None:intergenic
CGAAGAAATTCAAGGAATCA+TGG 0.453331 1:-40424765 MsaT002409.1:CDS
GACCAAACCTTGTTGCAACC+TGG 0.459346 1:+40420811 None:intergenic
CTGTTGGCTGAATAAAATCC+AGG 0.459891 1:+40417073 None:intergenic
AGATGAAGATGGAGCTATGA+AGG 0.464102 1:-40417237 MsaT002409.1:CDS
CATCCACCTTCTTCATTCTC+CGG 0.464454 1:+40424494 None:intergenic
CTGTCTGCTTGCAGCCACTC+TGG 0.465417 1:-40415692 MsaT002409.1:intron
AATGAAACTGTTGCAAGAGC+TGG 0.476800 1:-40420836 MsaT002409.1:CDS
ACAGCGGGTGAAACTGCGTT+AGG 0.478912 1:+40417736 None:intergenic
TGCTGTCTGTTTCCATTGTG+TGG 0.479482 1:+40417700 None:intergenic
CTTCTTCCATGCTAGCCTGT+TGG 0.482708 1:+40417057 None:intergenic
CAACTTTGTTTGAGATTGCA+GGG 0.485138 1:-40414901 MsaT002409.1:CDS
CAATTCAACGAAGAAATTCA+AGG 0.489910 1:-40424773 MsaT002409.1:CDS
GTGTTGCAAGTCAGTGGATA+GGG 0.490731 1:+40424635 None:intergenic
TGTCCTCTGTTTGTCAGAGT+CGG 0.492707 1:-40417104 MsaT002409.1:CDS
GGACTTTCAGTCACTTCGCT+AGG 0.493268 1:-40414835 MsaT002409.1:CDS
TTACCCTTTCTGCTAAGCTA+TGG 0.495150 1:+40424196 None:intergenic
ACCAAACCTTGTTGCAACCT+GGG 0.495296 1:+40420812 None:intergenic
TTCAAGATTCCATGATCCTA+TGG 0.496985 1:+40415042 None:intergenic
GCACTCAAATCAGATGAAGA+TGG 0.500237 1:-40417248 MsaT002409.1:CDS
GAGCAGGCACAACATTCAGC+AGG 0.501059 1:-40417134 MsaT002409.1:intron
TTGTGGACCGCGAATACTCA+TGG 0.507116 1:+40414921 None:intergenic
TCAACCAACGCGGCGTTGGA+AGG 0.508170 1:+40424596 None:intergenic
CTCTGTTTGTCAGAGTCGGT+TGG 0.512545 1:-40417100 MsaT002409.1:CDS
CTGTTGCAAGAGCTGGTCCC+AGG 0.513301 1:-40420829 MsaT002409.1:CDS
AGGAATCATGGCTCCGGCGA+CGG 0.514735 1:-40424753 MsaT002409.1:CDS
TCGATGCGCCATGCTTAAAG+AGG 0.517812 1:-40415570 MsaT002409.1:CDS
TAGCCATAGCTTAGCAGAAA+GGG 0.520877 1:-40424199 MsaT002409.1:intron
TGACGGTGCTAGTGTGCCTC+GGG 0.522536 1:-40414179 MsaT002409.1:intron
AGCTCGACGACCTGCGTCGG+TGG 0.526023 1:+40424695 None:intergenic
AAGGAAGGAACGAGAAAAGA+AGG 0.526931 1:-40424426 MsaT002409.1:intron
GGAATTTCTGGCCACACAGC+GGG 0.527591 1:+40417721 None:intergenic
CGCCATGCTTAAAGAGGCGT+TGG 0.527613 1:-40415564 MsaT002409.1:CDS
ATGACGGTGCTAGTGTGCCT+CGG 0.533157 1:-40414180 MsaT002409.1:CDS
CAGGAAGATCCATAGGATCA+TGG 0.542587 1:-40415051 MsaT002409.1:CDS
ACAGATATCAGGAACAGCAT+TGG 0.547071 1:-40419211 MsaT002409.1:intron
GGTGTTGCAAGTCAGTGGAT+AGG 0.551839 1:+40424634 None:intergenic
AATTCAAGGAATCATGGCTC+CGG 0.553332 1:-40424759 MsaT002409.1:CDS
CAACCAACGCGGCGTTGGAA+GGG 0.553760 1:+40424597 None:intergenic
CTGCAGCCAATATGCTGTCA+AGG 0.553773 1:+40418534 None:intergenic
AACATACCTATGCTCAAAGA+TGG 0.555740 1:-40414964 MsaT002409.1:CDS
TTTCCCTTCCAACGCCGCGT+TGG 0.556664 1:-40424600 MsaT002409.1:CDS
GTCTTACCCATGAGTATTCG+CGG 0.560460 1:-40414928 MsaT002409.1:CDS
ATTCAGCCAACAGGCTAGCA+TGG 0.560599 1:-40417063 MsaT002409.1:CDS
TGCAATCTCAAACAAAGTTG+TGG 0.564873 1:+40414904 None:intergenic
TTACTTATTAGCATGTCTCT+CGG 0.566454 1:+40417421 None:intergenic
CAGCGGGTGAAACTGCGTTA+GGG 0.566488 1:+40417737 None:intergenic
GCCGACGAGAATTCCGGCAA+CGG 0.567888 1:-40424273 MsaT002409.1:CDS
GAGCTACCGGAGAATGAAGA+AGG 0.569193 1:-40424500 MsaT002409.1:CDS
GATGAAGATGGAGCTATGAA+GGG 0.570787 1:-40417236 MsaT002409.1:CDS
GACTTTCAGTCACTTCGCTA+GGG 0.571195 1:-40414834 MsaT002409.1:intron
GCATATCCATCTTTGAGCAT+AGG 0.572316 1:+40414958 None:intergenic
TGAGGGATAAACAAATTGAA+AGG 0.572817 1:-40417455 MsaT002409.1:intron
GAAAGTTCTACCTGATAACA+AGG 0.573754 1:-40415525 MsaT002409.1:CDS
TTAAGTGCAGGAAGATCCAT+AGG 0.574773 1:-40415058 MsaT002409.1:intron
GCAGGTGAAGGATGAGCTAC+CGG 0.582554 1:-40424513 MsaT002409.1:CDS
CATTCTGTAGAGATTGCACA+TGG 0.585158 1:+40419169 None:intergenic
GTCAGTGGATAGGGTTTCGG+CGG 0.587492 1:+40424644 None:intergenic
ACTTCTAAGTTATGACTCAT+CGG 0.587921 1:-40417587 MsaT002409.1:CDS
GACCAACGCCTCTTTAAGCA+TGG 0.590815 1:+40415562 None:intergenic
GCCGTTGCCGGAATTCTCGT+CGG 0.594568 1:+40424272 None:intergenic
GCGTTCAACCAACGCGGCGT+TGG 0.594819 1:+40424592 None:intergenic
CAAGTATGGTGATCCAAACA+TGG 0.598714 1:+40415611 None:intergenic
ATTGTAGGGAATGTCTCTCA+TGG 0.599541 1:-40417770 MsaT002409.1:intron
GACAGCAATTTCAGCAATCG+TGG 0.599757 1:-40417684 MsaT002409.1:CDS
ATAGCCATAGCTTAGCAGAA+AGG 0.599793 1:-40424200 MsaT002409.1:CDS
TATCCACTGACTTGCAACAC+CGG 0.603238 1:-40424632 MsaT002409.1:CDS
CATTCCACCAACCACTTTGG+GGG 0.604196 1:-40417651 MsaT002409.1:CDS
CGAACTCGAACGTGAACGTA+CGG 0.605120 1:+40424240 None:intergenic
TGTGGACCGCGAATACTCAT+GGG 0.606633 1:+40414922 None:intergenic
CGTCGAGCTTCTTCACTCGT+CGG 0.607615 1:-40424681 MsaT002409.1:CDS
GCATTCCACCAACCACTTTG+GGG 0.608496 1:-40417652 MsaT002409.1:CDS
GAATTGAATCGACTCGAACG+TGG 0.610167 1:+40424790 None:intergenic
TCTTGAACTTCCACCGACGC+AGG 0.611122 1:-40424705 MsaT002409.1:CDS
TGGAATTTCTGGCCACACAG+CGG 0.611227 1:+40417720 None:intergenic
TTACCGGTGTTGCAAGTCAG+TGG 0.611762 1:+40424629 None:intergenic
GCAATCTCTACAGAATGAAG+TGG 0.617385 1:-40419163 MsaT002409.1:CDS
ATCTCTACAGAATGAAGTGG+AGG 0.623403 1:-40419160 MsaT002409.1:intron
GCTGAACTTACCGCAGGTGA+AGG 0.628100 1:-40424525 MsaT002409.1:CDS
TGGATGTGTCTCTGATCCAA+CGG 0.628322 1:-40424477 MsaT002409.1:CDS
ACGCAGTTTCACCCGCTGTG+TGG 0.639303 1:-40417732 MsaT002409.1:CDS
AAACTCGCTGAACTTACCGC+AGG 0.646946 1:-40424531 MsaT002409.1:CDS
AGAAGCTCGACGACCTGCGT+CGG 0.647459 1:+40424692 None:intergenic
GGCGAAAACGGAGAATCTGG+CGG 0.648507 1:+40424568 None:intergenic
AACTTTGTTTGAGATTGCAG+GGG 0.651392 1:-40414900 MsaT002409.1:CDS
CTACCGGAGAATGAAGAAGG+TGG 0.653905 1:-40424497 MsaT002409.1:CDS
GGATCATGGAATCTTGAACC+CGG 0.654002 1:-40415037 MsaT002409.1:CDS
CAAAGATGGATATGCATCCG+TGG 0.656445 1:-40414950 MsaT002409.1:CDS
TGGCCAGAAATTCCACACAA+TGG 0.658395 1:-40417712 MsaT002409.1:CDS
GCTGGTCCCAGGTTGCAACA+AGG 0.662336 1:-40420818 MsaT002409.1:intron
ATGAAGATGGAGCTATGAAG+GGG 0.663574 1:-40417235 MsaT002409.1:intron
CAACCGACTCTGACAAACAG+AGG 0.673114 1:+40417101 None:intergenic
GGTAGCTCATCCTTCACCTG+CGG 0.677655 1:+40424515 None:intergenic
TTGAGCATAGGTATGTTGCA+TGG 0.677840 1:+40414970 None:intergenic
CGGTGGAAGTTCAAGAAGTG+CGG 0.677977 1:+40424712 None:intergenic
GTTGCCGGAATTCTCGTCGG+CGG 0.688782 1:+40424275 None:intergenic
TAGGTATGTTGCATGGTGTG+AGG 0.706289 1:+40414977 None:intergenic
AAAGTTCTACCTGATAACAA+GGG 0.711936 1:-40415524 MsaT002409.1:intron
AATACTCATGGGTAAGACCA+CGG 0.728456 1:+40414933 None:intergenic
GGCGGCGCGTTCAACCAACG+CGG 0.742023 1:+40424586 None:intergenic
TTTCACTAACAAGACCAGAG+TGG 0.755614 1:+40415678 None:intergenic
GTTCACGTTCGAGTTCGACG+AGG 0.757130 1:-40424234 MsaT002409.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 40413830 40424822 40413830 ID=MsaG002409
Chr1 mRNA 40413830 40424822 40413830 ID=MsaT002409.1;Parent=MsaG002409
Chr1 exon 40413830 40413839 40413830 ID=MsaT002409.1.exon13;Parent=MsaT002409.1
Chr1 CDS 40413830 40413839 40413830 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40414180 40414220 40414180 ID=MsaT002409.1.exon12;Parent=MsaT002409.1
Chr1 CDS 40414180 40414220 40414180 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40414835 40415070 40414835 ID=MsaT002409.1.exon11;Parent=MsaT002409.1
Chr1 CDS 40414835 40415070 40414835 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40415525 40415700 40415525 ID=MsaT002409.1.exon10;Parent=MsaT002409.1
Chr1 CDS 40415525 40415700 40415525 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40417052 40417150 40417052 ID=MsaT002409.1.exon9;Parent=MsaT002409.1
Chr1 CDS 40417052 40417150 40417052 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40417236 40417277 40417236 ID=MsaT002409.1.exon8;Parent=MsaT002409.1
Chr1 CDS 40417236 40417277 40417236 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40417425 40417473 40417425 ID=MsaT002409.1.exon7;Parent=MsaT002409.1
Chr1 CDS 40417425 40417473 40417425 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40417578 40417785 40417578 ID=MsaT002409.1.exon6;Parent=MsaT002409.1
Chr1 CDS 40417578 40417785 40417578 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40418532 40418603 40418532 ID=MsaT002409.1.exon5;Parent=MsaT002409.1
Chr1 CDS 40418532 40418603 40418532 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40419161 40419229 40419161 ID=MsaT002409.1.exon4;Parent=MsaT002409.1
Chr1 CDS 40419161 40419229 40419161 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40420819 40420884 40420819 ID=MsaT002409.1.exon3;Parent=MsaT002409.1
Chr1 CDS 40420819 40420884 40420819 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40424200 40424331 40424200 ID=MsaT002409.1.exon2;Parent=MsaT002409.1
Chr1 CDS 40424200 40424331 40424200 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Chr1 exon 40424427 40424822 40424427 ID=MsaT002409.1.exon1;Parent=MsaT002409.1
Chr1 CDS 40424427 40424822 40424427 ID=cds.MsaT002409.1;Parent=MsaT002409.1
Gene Sequence

>MsaT002409.1

ATGGAACAAACCACGTTCGAGTCGATTCAATTCAACGAAGAAATTCAAGGAATCATGGCTCCGGCGACGGAAACAGCGAACTCTTTCACCGCACTTCTTGAACTTCCACCGACGCAGGTCGTCGAGCTTCTTCACTCGTCGGAGATTACCGGAAAACCGCCGAAACCCTATCCACTGACTTGCAACACCGGTAATTTAACTTTCCCTTCCAACGCCGCGTTGGTTGAACGCGCCGCCAGATTCTCCGTTTTCGCCGGAGAAAATTCCACAAACTCGCTGAACTTACCGCAGGTGAAGGATGAGCTACCGGAGAATGAAGAAGGTGGATGTGTCTCTGATCCAACGGTCGAAAACACGAATTCCAAGAGTGCAAAAAGGAAGGAACGAGAAAAGAAGGTGAAATTGTCGTCTAAGAAGAGTAAGAGTATCGCCGCCGACGAGAATTCCGGCAACGGCGATGAGCTTCCGTACGTTCACGTTCGAGTTCGACGAGGTCAAGCTACTGATAGCCATAGCTTAGCAGAAAGGGCTAGGAGAGAGAAGATAAATGCTCGAATGAAACTGTTGCAAGAGCTGGTCCCAGGTTGCAACAAGATATCAGGAACAGCATTGGTTTTGGATAAAATCATCAACCATGTGCAATCTCTACAGAATGAAGTGGAGATTTTATCAATGAAACTGGCAGCAGTTAACCCAATAATTGATTTCAACCTTGACAGCATATTGGCTGCAGAAGGAATGTCTCTCATGGACAGTAACTTCCCTAACGCAGTTTCACCCGCTGTGTGGCCAGAAATTCCACACAATGGAAACAGACAGCAATTTCAGCAATCGTGGCAATCTGATGCATTCCACCAACCACTTTGGGGGAGAGAAGAAAACAACCCTAATTTCATGACCCCAGAAAACTCACTTCTAAGTTATGACTCATCGGCAAATTCAGGGATAAACAAATTGAAAGGCATAAAGAAATCCGAGAGACATGCTAATAATATCTCTGCACTCAAATCAGATGAAGATGGAGCTATGAAGGGGCACAACATTCAGCAGGATTTCATTGTCCTCTGTTTGTCAGAGTCGGTTGGAAAACCTGGATTTTATTCAGCCAACAGGCTAGCATGGAAGAAGAATTCCCACTCTGGTCTTGTTAGTGAAATCTGCAAAACTTATTATTCTATGAAATGGGTTTCTGGTCTTGAACCATGTTTGGATCACCATACTTGTATCATCAATCTTTTTAGTCGATGCGCCATGCTTAAAGAGGCGTTGGTCGTAATATTGAAAATGAAAGTTCTACCTGATAACAAGGGAAGATCCATAGGATCATGGAATCTTGAACCCGGTTTGTTGAAAACTTTTGCTTGGACTACTGATTTCAATCCTCACACCATGCAACATACCTATGCTCAAAGATGGATATGCATCCGTGGTCTTACCCATGAGTATTCGCGGTCCACAACTTTGTTTGAGATTGCAGGGGCATTAGACACTTCTTTGACTTTTGATAAGGCCACTAAGAATAGGACTTTCAGTCACTTCGCTAGGTCGTTTTGGAAGACAAAGATGACGGTGCTAGTGTGCCTCGGGGTGTTGTAG
Protein sequence

>MsaT002409.1

MEQTTFESIQFNEEIQGIMAPATETANSFTALLELPPTQVVELLHSSEITGKPPKPYPLTCNTGNLTFPSNAALVERAARFSVFAGENSTNSLNLPQVKDELPENEEGGCVSDPTVENTNSKSAKRKEREKKVKLSSKKSKSIAADENSGNGDELPYVHVRVRRGQATDSHSLAERARREKINARMKLLQELVPGCNKISGTALVLDKIINHVQSLQNEVEILSMKLAAVNPIIDFNLDSILAAEGMSLMDSNFPNAVSPAVWPEIPHNGNRQQFQQSWQSDAFHQPLWGREENNPNFMTPENSLLSYDSSANSGINKLKGIKKSERHANNISALKSDEDGAMKGHNIQQDFIVLCLSESVGKPGFYSANRLAWKKNSHSGLVSEICKTYYSMKWVSGLEPCLDHHTCIINLFSRCAMLKEALVVILKMKVLPDNKGRSIGSWNLEPGLLKTFAWTTDFNPHTMQHTYAQRWICIRGLTHEYSRSTTLFEIAGALDTSLTFDKATKNRTFSHFARSFWKTKMTVLVCLGVL*