AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002420 MtrunA17_Chr1g0174271 91.821 379 19 4 2 372 4 378 0.0 694
MsaG002420 MtrunA17_Chr2g0329041 41.745 424 182 12 1 372 1 411 5.27e-85 264
MsaG002420 MtrunA17_Chr7g0258181 45.213 376 133 8 1 364 1 315 4.26e-82 253
MsaG002420 MtrunA17_Chr4g0032951 50.958 261 107 8 121 369 181 432 6.42e-76 241
MsaG002420 MtrunA17_Chr3g0087771 37.129 404 185 15 7 369 24 399 5.30e-56 188
MsaG002420 MtrunA17_Chr8g0351881 38.830 376 193 9 7 364 32 388 5.18e-52 177
MsaG002420 MtrunA17_Chr4g0014771 70.787 89 24 1 276 364 104 190 1.69e-38 136
MsaG002420 MtrunA17_Chr1g0213791 42.353 255 124 9 120 364 96 337 2.10e-38 140
MsaG002420 MtrunA17_Chr4g0074161 59.420 69 27 1 291 359 205 272 3.51e-18 85.5
MsaG002420 MtrunA17_Chr1g0190731 53.333 75 34 1 285 359 186 259 5.16e-17 81.6
MsaG002420 MtrunA17_Chr1g0152131 53.425 73 34 0 290 362 258 330 8.59e-17 81.3
MsaG002420 MtrunA17_Chr3g0131771 56.716 67 29 0 292 358 147 213 6.43e-16 77.8
MsaG002420 MtrunA17_Chr5g0421111 53.521 71 33 0 292 362 365 435 1.31e-15 78.2
MsaG002420 MtrunA17_Chr3g0144641 55.072 69 31 0 292 360 191 259 1.89e-15 77.0
MsaG002420 MtrunA17_Chr7g0234761 53.623 69 32 0 292 360 317 385 3.21e-15 77.0
MsaG002420 MtrunA17_Chr8g0365941 53.623 69 32 0 292 360 305 373 4.06e-15 76.6
MsaG002420 MtrunA17_Chr8g0339091 53.731 67 31 0 292 358 291 357 8.86e-15 75.5
MsaG002420 MtrunA17_Chr4g0035101 50.000 76 36 1 285 360 138 211 1.32e-14 74.3
MsaG002420 MtrunA17_Chr8g0385911 52.174 69 33 0 292 360 145 213 1.53e-14 73.9
MsaG002420 MtrunA17_Chr8g0363921 50.746 67 33 0 292 358 151 217 8.73e-14 70.9
MsaG002420 MtrunA17_Chr5g0415291 49.254 67 34 0 292 358 112 178 1.28e-12 67.0
MsaG002420 MtrunA17_Chr7g0258161 46.377 69 37 0 292 360 173 241 3.90e-12 66.6
MsaG002420 MtrunA17_Chr1g0152651 54.545 66 29 1 291 356 273 337 4.67e-12 66.6
MsaG002420 MtrunA17_Chr3g0134001 42.697 89 46 2 274 358 149 236 5.81e-12 65.5
MsaG002420 MtrunA17_Chr3g0134741 54.545 66 29 1 291 356 228 292 6.64e-12 65.9
MsaG002420 MtrunA17_Chr1g0174161 44.928 69 38 0 292 360 160 228 1.43e-11 65.1
MsaG002420 MtrunA17_Chr6g0484271 46.269 67 36 0 292 358 155 221 2.81e-11 63.5
MsaG002420 MtrunA17_Chr1g0184841 53.333 75 34 1 285 359 113 186 5.58e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002420 AT2G42280.1 47.558 389 151 7 1 369 1 356 2.48e-99 299
MsaG002420 AT2G42280.3 47.185 373 144 7 1 353 1 340 6.03e-91 278
MsaG002420 AT2G42280.2 41.337 329 140 7 1 309 1 296 6.10e-58 191
MsaG002420 AT1G51140.1 36.504 389 189 13 7 364 15 376 2.41e-57 191
MsaG002420 AT1G05805.1 42.510 247 107 10 123 364 139 355 5.41e-44 156
MsaG002420 AT1G35460.1 70.115 87 24 1 278 364 169 253 1.41e-39 141
MsaG002420 AT2G43140.1 62.857 105 38 1 261 364 102 206 2.96e-38 137
MsaG002420 AT2G43140.2 42.353 255 111 10 123 369 82 308 3.48e-38 139
MsaG002420 AT4G09180.1 67.045 88 27 1 278 365 172 257 7.32e-36 132
MsaG002420 AT1G35460.2 79.245 53 9 1 278 330 169 219 1.43e-23 98.2
MsaG002420 AT2G24260.2 60.870 69 26 1 291 359 34 101 6.42e-19 85.5
MsaG002420 AT2G24260.1 60.870 69 26 1 291 359 137 204 2.12e-18 85.9
MsaG002420 AT4G30980.1 57.333 75 30 2 285 359 124 196 4.93e-18 84.3
MsaG002420 AT4G30980.2 57.333 75 30 2 285 359 172 244 1.00e-17 84.0
MsaG002420 AT5G58010.1 55.072 69 30 1 291 359 98 165 4.97e-17 81.3
MsaG002420 AT4G34530.1 56.522 69 30 0 292 360 172 240 2.37e-16 79.7
MsaG002420 AT1G10120.2 54.930 71 32 0 292 362 206 276 5.98e-16 79.0
MsaG002420 AT1G10120.1 54.930 71 32 0 292 362 206 276 5.98e-16 79.0
MsaG002420 AT5G50915.3 56.716 67 29 0 292 358 136 202 8.79e-16 77.0
MsaG002420 AT5G50915.4 56.716 67 29 0 292 358 136 202 8.79e-16 77.0
MsaG002420 AT5G50915.1 56.716 67 29 0 292 358 136 202 1.06e-15 77.0
MsaG002420 AT5G50915.2 56.716 67 29 0 292 358 136 202 1.06e-15 77.0
MsaG002420 AT1G68920.3 52.113 71 34 0 292 362 302 372 2.23e-15 77.8
MsaG002420 AT1G68920.1 52.113 71 34 0 292 362 303 373 2.39e-15 77.8
MsaG002420 AT1G68920.4 52.113 71 34 0 292 362 303 373 2.39e-15 77.8
MsaG002420 AT1G68920.2 52.113 71 34 0 292 362 303 373 2.39e-15 77.8
MsaG002420 AT1G25330.1 50.000 76 37 1 283 358 96 170 5.47e-15 73.9
MsaG002420 AT1G26260.5 55.224 67 30 0 292 358 201 267 1.05e-14 75.1
MsaG002420 AT3G07340.2 53.731 67 31 0 292 358 258 324 1.21e-14 75.5
MsaG002420 AT3G07340.1 53.731 67 31 0 292 358 258 324 1.26e-14 75.5
MsaG002420 AT1G26260.4 55.224 67 30 0 292 358 223 289 1.34e-14 75.1
MsaG002420 AT1G26260.1 55.224 67 30 0 292 358 223 289 1.34e-14 75.1
MsaG002420 AT1G26260.6 55.224 67 30 0 292 358 223 289 1.34e-14 75.1
MsaG002420 AT1G26260.2 55.224 67 30 0 292 358 223 289 1.34e-14 75.1
MsaG002420 AT1G26260.3 55.224 67 30 0 292 358 172 238 1.36e-14 74.7
MsaG002420 AT5G48560.1 47.368 76 40 0 292 367 301 376 1.84e-14 75.1
MsaG002420 AT5G48560.2 47.368 76 40 0 292 367 301 376 1.91e-14 74.7
MsaG002420 AT3G23690.1 50.725 69 34 0 292 360 191 259 1.06e-13 72.0
MsaG002420 AT1G59640.1 50.746 67 33 0 292 358 136 202 2.51e-13 69.7
MsaG002420 AT2G42300.1 46.053 76 40 1 286 360 178 253 2.81e-13 70.5
MsaG002420 AT2G42300.4 46.053 76 40 1 286 360 178 253 3.10e-13 69.7
MsaG002420 AT1G18400.2 49.254 67 34 0 292 358 145 211 3.19e-13 69.3
MsaG002420 AT1G73830.2 49.254 67 34 0 292 358 147 213 3.95e-13 69.3
MsaG002420 AT1G73830.1 49.254 67 34 0 292 358 147 213 4.23e-13 69.3
MsaG002420 AT1G18400.1 49.254 67 34 0 292 358 145 211 4.82e-13 68.9
MsaG002420 AT2G18300.1 47.826 69 36 0 292 360 185 253 6.53e-13 69.3
MsaG002420 AT2G18300.2 47.826 69 36 0 292 360 185 253 6.62e-13 69.3
MsaG002420 AT4G36540.2 45.455 77 40 1 282 358 133 207 6.93e-13 68.9
MsaG002420 AT4G36540.1 45.455 77 40 1 282 358 133 207 6.99e-13 68.9
MsaG002420 AT1G59640.2 50.746 67 33 0 292 358 136 202 8.98e-13 69.3
MsaG002420 AT3G57800.2 46.479 71 38 0 290 360 202 272 1.04e-12 69.3
MsaG002420 AT2G18300.3 47.826 69 36 0 292 360 185 253 1.04e-12 68.9
MsaG002420 AT1G27740.1 52.857 70 32 1 287 356 162 230 5.11e-12 65.9
MsaG002420 AT4G33880.1 54.545 66 29 1 291 356 265 329 5.70e-12 66.6
MsaG002420 AT2G14760.1 54.545 66 29 1 291 356 236 300 5.95e-12 66.6
MsaG002420 AT5G62610.2 46.269 67 36 0 292 358 153 219 1.01e-11 65.5
MsaG002420 AT5G62610.1 46.269 67 36 0 292 358 153 219 1.01e-11 65.5
MsaG002420 AT4G02590.2 53.333 75 34 1 285 359 139 212 5.50e-11 63.5
MsaG002420 AT4G02590.1 53.333 75 34 1 285 359 139 212 5.50e-11 63.5
MsaG002420 AT4G02590.3 53.333 75 34 1 285 359 76 149 6.18e-11 62.8

Find 69 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGCTATGGATGGTTCTTT+TGG 0.221242 1:+40714992 MsaT002420.1:CDS
CACAACCAATCCAATTCTTC+TGG 0.235104 1:+40714734 MsaT002420.1:CDS
GGAAACTATGCCACGGTAAA+TGG 0.245415 1:+40716177 MsaT002420.1:CDS
TGTTCTAAAGAAATGAATTC+TGG 0.255357 1:+40714911 MsaT002420.1:CDS
TCCAACATTTCCTTTCAAAA+TGG 0.262412 1:+40715112 MsaT002420.1:CDS
CTGGATTCCCCTATAGTTCT+TGG 0.264196 1:+40716376 MsaT002420.1:CDS
ACATCACATGATAAGAGTTT+TGG 0.326645 1:+40715046 MsaT002420.1:CDS
ATTTGTACCTTATCCATGTT+TGG 0.331656 1:-40716992 None:intergenic
TTTGGATTGGTTGGTTCATT+GGG 0.337311 1:+40715010 MsaT002420.1:CDS
ACGAAGCAACCCAGAAGAAT+TGG 0.359803 1:-40714744 None:intergenic
CCATTGCTACCTCCGTCATC+AGG 0.366384 1:-40716333 None:intergenic
AAAGAAATGAATTCTGGTTA+TGG 0.376238 1:+40714917 MsaT002420.1:CDS
ATAGTTTCCAACACCTTTCA+TGG 0.387270 1:-40716163 None:intergenic
TCAAGAAATGCTTCTTCGTT+GGG 0.389766 1:+40716258 MsaT002420.1:CDS
ACTCATCAGCGTGGAGGGCT+TGG 0.396464 1:+40716568 MsaT002420.1:intron
GTGTCGTTCCAAGAACTATA+GGG 0.397091 1:-40716384 None:intergenic
ATTGGTTGGTTCATTGGGAA+TGG 0.419938 1:+40715015 MsaT002420.1:CDS
CATTATCCTAGACATGGTTC+TGG 0.422292 1:+40714956 MsaT002420.1:CDS
TGGTTCAAATCTTCTTAGAC+AGG 0.423641 1:+40715066 MsaT002420.1:CDS
AGTGTCGTTCCAAGAACTAT+AGG 0.434493 1:-40716385 None:intergenic
TTCTACTTCAAGTTCTGCTA+TGG 0.441169 1:+40714979 MsaT002420.1:CDS
TTCAAGAAATGCTTCTTCGT+TGG 0.443867 1:+40716257 MsaT002420.1:CDS
ACAACCAATCCAATTCTTCT+GGG 0.461387 1:+40714735 MsaT002420.1:CDS
AACGAAGAAGCATTTCTTGA+AGG 0.468589 1:-40716255 None:intergenic
ACCCTCTCGGCAATGCTTCG+AGG 0.468775 1:-40716727 None:intergenic
ATCATCTCAGATGAAGTGTT+TGG 0.470848 1:-40716628 None:intergenic
ACTTCAAGTTCTGCTATGGA+TGG 0.477883 1:+40714983 MsaT002420.1:CDS
ATGAATTCTGGTTATGGTGC+TGG 0.478438 1:+40714923 MsaT002420.1:CDS
TCCTCGAAGCATTGCCGAGA+GGG 0.481258 1:+40716726 MsaT002420.1:CDS
AATGGCGATTCTACTCACTA+TGG 0.489783 1:+40716351 MsaT002420.1:CDS
GCTTGTTCCAAACATGGATA+AGG 0.495622 1:+40716985 MsaT002420.1:CDS
TCACCAGAAGAAGGAAAAGA+AGG 0.496661 1:-40714806 None:intergenic
ACGAAGAAGCATTTCTTGAA+GGG 0.507126 1:-40716254 None:intergenic
GATTCTACTCACTATGGCTC+TGG 0.511407 1:+40716357 MsaT002420.1:CDS
CACATCAGACATGTTGGACT+TGG 0.512180 1:+40717125 MsaT002420.1:CDS
ATGTTGGAGAAAAGACCAGC+AGG 0.517169 1:-40715097 None:intergenic
ATCTCAGTGGTTTGAAAAGA+GGG 0.517303 1:+40716424 MsaT002420.1:CDS
AATCTCAGTGGTTTGAAAAG+AGG 0.526692 1:+40716423 MsaT002420.1:CDS
CCACCTCATTATCCTAGACA+TGG 0.529372 1:+40714950 MsaT002420.1:CDS
TTATTAGAATCACCAGAAGA+AGG 0.532913 1:-40714815 None:intergenic
GCAACCCAGAAGAATTGGAT+TGG 0.541202 1:-40714739 None:intergenic
AGACAGGGTAGTTCCCCTGC+TGG 0.543236 1:+40715082 MsaT002420.1:CDS
AACTAACACATCAGACATGT+TGG 0.546138 1:+40717119 MsaT002420.1:CDS
GCAAGAGCTTGTTCCAAACA+TGG 0.548846 1:+40716979 MsaT002420.1:CDS
GCCACGGTAAATGGTAGTAA+TGG 0.558605 1:+40716186 MsaT002420.1:CDS
CTAAGATCAGTGAAAGAATG+AGG 0.559063 1:+40716951 MsaT002420.1:CDS
AGCTGAGTTGAGTGTTCAGT+CGG 0.564670 1:-40716230 None:intergenic
TGTAAAATTCGAGCGAAACG+AGG 0.565652 1:+40716691 MsaT002420.1:CDS
TTCATCTGAGATGATTGCTA+TGG 0.566845 1:+40716636 MsaT002420.1:CDS
GAGCAAACTGCAAATGTATG+AGG 0.578835 1:+40717845 MsaT002420.1:CDS
CCTGATGACGGAGGTAGCAA+TGG 0.590547 1:+40716333 MsaT002420.1:CDS
CATTGCTACCTCCGTCATCA+GGG 0.595144 1:-40716332 None:intergenic
GTAGAACCAGAACCATGTCT+AGG 0.597203 1:-40714962 None:intergenic
GAACCATGTCTAGGATAATG+AGG 0.601649 1:-40714953 None:intergenic
TAAACAAAACCATACCCTCT+CGG 0.609076 1:-40716740 None:intergenic
GTGTTCAGTCGGTTAATAGA+CGG 0.618315 1:-40716219 None:intergenic
CATCTCAGAAATAGATAGTG+AGG 0.622320 1:+40716293 MsaT002420.1:CDS
GCCATTACTACCATTTACCG+TGG 0.622329 1:-40716187 None:intergenic
GGTTCAAATCTTCTTAGACA+GGG 0.631058 1:+40715067 MsaT002420.1:CDS
GAAGCAACTAGCCCTGATGA+CGG 0.637823 1:+40716321 MsaT002420.1:CDS
TGTCGTTCCAAGAACTATAG+GGG 0.638078 1:-40716383 None:intergenic
TGTTGGAGAAAAGACCAGCA+GGG 0.641864 1:-40715096 None:intergenic
GTTGGAGAAAAGACCAGCAG+GGG 0.676212 1:-40715095 None:intergenic
AGGTGTTGGAAACTATGCCA+CGG 0.680755 1:+40716170 MsaT002420.1:CDS
ATCCTCGAAGCATTGCCGAG+AGG 0.702830 1:+40716725 MsaT002420.1:CDS
CAAATGTATGAGGATGCAGA+AGG 0.707448 1:+40717855 MsaT002420.1:CDS
GCAACTAGCCCTGATGACGG+AGG 0.713476 1:+40716324 MsaT002420.1:CDS
CCATGTCTAGGATAATGAGG+TGG 0.720480 1:-40714950 None:intergenic
TCATTCACAGAAAATCTCAG+TGG 0.729969 1:+40716411 MsaT002420.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 40714710 40717894 40714710 ID=MsaG002420
Chr1 mRNA 40714710 40717894 40714710 ID=MsaT002420.1;Parent=MsaG002420
Chr1 exon 40714710 40715133 40714710 ID=MsaT002420.1.exon1;Parent=MsaT002420.1
Chr1 CDS 40714710 40715133 40714710 ID=cds.MsaT002420.1;Parent=MsaT002420.1
Chr1 exon 40716154 40716482 40716154 ID=MsaT002420.1.exon2;Parent=MsaT002420.1
Chr1 CDS 40716154 40716482 40716154 ID=cds.MsaT002420.1;Parent=MsaT002420.1
Chr1 exon 40716577 40716747 40716577 ID=MsaT002420.1.exon3;Parent=MsaT002420.1
Chr1 CDS 40716577 40716747 40716577 ID=cds.MsaT002420.1;Parent=MsaT002420.1
Chr1 exon 40716941 40717006 40716941 ID=MsaT002420.1.exon4;Parent=MsaT002420.1
Chr1 CDS 40716941 40717006 40716941 ID=cds.MsaT002420.1;Parent=MsaT002420.1
Chr1 exon 40717117 40717185 40717117 ID=MsaT002420.1.exon5;Parent=MsaT002420.1
Chr1 CDS 40717117 40717185 40717117 ID=cds.MsaT002420.1;Parent=MsaT002420.1
Chr1 exon 40717829 40717894 40717829 ID=MsaT002420.1.exon6;Parent=MsaT002420.1
Chr1 CDS 40717829 40717894 40717829 ID=cds.MsaT002420.1;Parent=MsaT002420.1
Gene Sequence

>MsaT002420.1

ATGGATTCTAATACTCATCAACAACACAACCAATCCAATTCTTCTGGGTTGCTTCGTTTTCGTTCTGCACCTAGTTCACTGTTTCAAAATCTCACACCTTCTTTTCCTTCTTCTGGTGATTCTAATAAAGTTACTAAACATTTTTGGGATGGTTCTGAATCAGAGAGATTTGTGAGTAACGATAATATTTCTAGTTCATCTTGTTCTAAAGAAATGAATTCTGGTTATGGTGCTGGTTTGCCACCTCATTATCCTAGACATGGTTCTGGTTCTACTTCAAGTTCTGCTATGGATGGTTCTTTTGGATTGGTTGGTTCATTGGGAATGGATCAAGAAACATCACATGATAAGAGTTTTGGTTCAAATCTTCTTAGACAGGGTAGTTCCCCTGCTGGTCTTTTCTCCAACATTTCCTTTCAAAATGGGTTTTCTACCATGAAAGGTGTTGGAAACTATGCCACGGTAAATGGTAGTAATGGCGAACTTAGTCCGTCTATTAACCGACTGAACACTCAACTCAGCTTCCCTTCAAGAAATGCTTCTTCGTTGGGAATATTGTCACACATCTCAGAAATAGATAGTGAGGACATTGAAGCAACTAGCCCTGATGACGGAGGTAGCAATGGCGATTCTACTCACTATGGCTCTGGATTCCCCTATAGTTCTTGGAACGACACTCAATCATTCACAGAAAATCTCAGTGGTTTGAAAAGAGGGCGAAGCAGTAATGAAAAAATGTTTTCTGATATTCAGCGTGGAGGGCTTGGAAATCAAGTTCATACATTATCGCATCAGTTGAGTTTACCAAACACTTCATCTGAGATGATTGCTATGGAGAAGTTGTTTCAGTTTCCAGATTCTGTTCCTTGTAAAATTCGAGCGAAACGAGGTTGCGCTACTCATCCTCGAAGCATTGCCGAGAGGGTAAGAAGAACTAAGATCAGTGAAAGAATGAGGAAATTGCAAGAGCTTGTTCCAAACATGGATAAGCAAACTAACACATCAGACATGTTGGACTTGGCTGTTGATTACATCAAAAATCTTCAAAAACAATTCAAGTCCCTAAGTGAAAAACGAGCAAACTGCAAATGTATGAGGATGCAGAAGGCAGAACAAAGAAGCTAA

Protein sequence

>MsaT002420.1

MDSNTHQQHNQSNSSGLLRFRSAPSSLFQNLTPSFPSSGDSNKVTKHFWDGSESERFVSNDNISSSSCSKEMNSGYGAGLPPHYPRHGSGSTSSSAMDGSFGLVGSLGMDQETSHDKSFGSNLLRQGSSPAGLFSNISFQNGFSTMKGVGNYATVNGSNGELSPSINRLNTQLSFPSRNASSLGILSHISEIDSEDIEATSPDDGGSNGDSTHYGSGFPYSSWNDTQSFTENLSGLKRGRSSNEKMFSDIQRGGLGNQVHTLSHQLSLPNTSSEMIAMEKLFQFPDSVPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQELVPNMDKQTNTSDMLDLAVDYIKNLQKQFKSLSEKRANCKCMRMQKAEQRS*