AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002455


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002455 MtrunA17_Chr1g0174861 71.600 250 43 4 20 260 16 246 1.60e-117 337
MsaG002455 MtrunA17_Chr1g0174871 60.364 275 84 7 20 278 20 285 1.40e-92 275
MsaG002455 MtrunA17_Chr1g0169521 36.486 296 131 9 29 277 30 315 1.22e-41 145
MsaG002455 MtrunA17_Chr1g0169561 34.576 295 136 8 29 276 30 314 1.83e-38 137
MsaG002455 MtrunA17_Chr1g0174001 39.500 200 87 7 106 277 1 194 3.02e-27 104
MsaG002455 MtrunA17_Chr1g0172961 32.636 239 129 9 29 238 23 258 5.39e-25 100
MsaG002455 MtrunA17_Chr1g0172861 31.868 273 129 13 29 268 23 271 1.27e-23 97.1
MsaG002455 MtrunA17_Chr7g0214511 33.600 250 111 10 49 277 71 286 1.10e-22 94.7
MsaG002455 MtrunA17_Chr4g0037041 41.270 126 57 5 28 144 22 139 5.92e-19 82.4
MsaG002455 MtrunA17_Chr5g0448221 34.177 158 73 8 33 166 54 204 1.60e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGGTCTCGTATCTTAT+TGG 0.208428 1:+41595647 None:intergenic
AGCACCAACAACTTTCTTAA+AGG 0.236987 1:-41595869 MsaT002455.1:CDS
TACCCTTCTTCGAATCATTT+TGG 0.242767 1:+41595726 None:intergenic
AAACAAAATTAAACACTAAA+TGG 0.270394 1:+41595223 None:intergenic
ATAATCCCAGCTCCGTTTCT+TGG 0.287583 1:+41595631 None:intergenic
GTTCTCTACATTGTTATCAT+TGG 0.335954 1:+41595361 None:intergenic
GTTTGAGTAATAAGTCCTTT+TGG 0.343516 1:+41595410 None:intergenic
TGAATCACCGTGACTGTAAA+AGG 0.346155 1:-41595532 MsaT002455.1:CDS
TGTTAACATTGCTGAAATAA+TGG 0.391363 1:-41595591 MsaT002455.1:CDS
TTTACCTTTAAGAAAGTTGT+TGG 0.419418 1:+41595865 None:intergenic
AACAAACACAAACATGCTAA+TGG 0.425038 1:-41595839 MsaT002455.1:CDS
TAAGAAAGTTGTTGGTGCTA+TGG 0.440297 1:+41595873 None:intergenic
CTCTACATTGTTATCATTGG+AGG 0.445625 1:+41595364 None:intergenic
CTTTGAATGGACACATGAAA+TGG 0.459517 1:-41595754 MsaT002455.1:CDS
TTGGTCTCGTATCTTATTGG+TGG 0.473127 1:+41595650 None:intergenic
ACGGTGATTCACATTAAGAT+TGG 0.473501 1:+41595544 None:intergenic
CAATGGATGGAAACTGATCT+AGG 0.474117 1:+41595690 None:intergenic
ATCCGTTTCATCACCTTCAT+CGG 0.483860 1:+41595334 None:intergenic
TGGTGCTATGGAATTGTTTG+TGG 0.484496 1:+41595885 None:intergenic
TGTGCTAGAGAATTCACAAC+TGG 0.492819 1:-41595923 MsaT002455.1:CDS
CAAATACTTTGTCAGATCAA+TGG 0.494617 1:+41595673 None:intergenic
GAATTGTTTGTGGCCGCCGA+GGG 0.497780 1:+41595895 None:intergenic
TGAATAGAATTATGAGAAGA+AGG 0.504138 1:+41596025 None:intergenic
TTCATGTGTTCTTTGCAGCA+AGG 0.509354 1:-41595795 MsaT002455.1:CDS
CAGCCAAAATGATTCGAAGA+AGG 0.519454 1:-41595729 MsaT002455.1:CDS
TACCGATGAAGGTGATGAAA+CGG 0.519602 1:-41595336 MsaT002455.1:CDS
CATGCTAATGGTATTAAGAT+CGG 0.524324 1:-41595827 MsaT002455.1:CDS
GTGAGATTAAAGATTCCAAA+AGG 0.530313 1:-41595425 MsaT002455.1:CDS
ACATTGTTATCATTGGAGGC+AGG 0.530674 1:+41595368 None:intergenic
ACGAGACCAAGAAACGGAGC+TGG 0.539882 1:-41595637 MsaT002455.1:CDS
GGAATTGTTTGTGGCCGCCG+AGG 0.556033 1:+41595894 None:intergenic
AGCCAAAATGATTCGAAGAA+GGG 0.558347 1:-41595728 MsaT002455.1:CDS
TTCACAACTGGTAATGCCCT+CGG 0.558819 1:-41595911 MsaT002455.1:CDS
ATGAAGGTGATGAAACGGAT+GGG 0.561647 1:-41595331 MsaT002455.1:CDS
GCAAAGAACACATGAATGGC+TGG 0.562308 1:+41595802 None:intergenic
GTAGAGAACAATACCGATGA+AGG 0.568109 1:-41595347 MsaT002455.1:CDS
CGAGACCAAGAAACGGAGCT+GGG 0.594941 1:-41595636 MsaT002455.1:CDS
CAATGAAAAGAACAGGTTGG+TGG 0.599420 1:-41595447 MsaT002455.1:CDS
CAAAATTAAACACTAAATGG+TGG 0.600626 1:+41595226 None:intergenic
TACTTTGTCAGATCAATGGA+TGG 0.603001 1:+41595677 None:intergenic
TGAAGGTGATGAAACGGATG+GGG 0.603593 1:-41595330 MsaT002455.1:CDS
TAAGATACGAGACCAAGAAA+CGG 0.610128 1:-41595643 MsaT002455.1:CDS
GATGAAGGTGATGAAACGGA+TGG 0.616100 1:-41595332 MsaT002455.1:CDS
AAACAATGAAAAGAACAGGT+TGG 0.620052 1:-41595450 MsaT002455.1:CDS
TGCTGCAAAGAACACATGAA+TGG 0.640126 1:+41595798 None:intergenic
TAACATTGCTGAAATAATGG+TGG 0.655107 1:-41595588 MsaT002455.1:CDS
ACAACTGGTAATGCCCTCGG+CGG 0.704742 1:-41595908 MsaT002455.1:CDS
GAAAATGAGAAACATCAACA+AGG 0.726705 1:-41595971 MsaT002455.1:CDS
GATGTGAAGATCAATGAACA+AGG 0.760498 1:-41595479 MsaT002455.1:CDS
GTGAAGATCAATGAACAAGG+TGG 0.773369 1:-41595476 MsaT002455.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 41595238 41596077 41595238 ID=MsaG002455
Chr1 mRNA 41595238 41596077 41595238 ID=MsaT002455.1;Parent=MsaG002455
Chr1 exon 41595238 41596077 41595238 ID=MsaT002455.1.exon1;Parent=MsaT002455.1
Chr1 CDS 41595238 41596077 41595238 ID=cds.MsaT002455.1;Parent=MsaT002455.1
Gene Sequence

>MsaT002455.1

ATGATGAAGAATTCTTTTTCTTCTTCTTCACCTTCTTCTCATAATTCTATTCATCATAAGCAAAAAAGCAATGTTGTTGATCATGAAAATGAGAAACATCAACAAGGTGCAAGACTTCACAAATGTGATTTGTGTGCTAGAGAATTCACAACTGGTAATGCCCTCGGCGGCCACAAACAATTCCATAGCACCAACAACTTTCTTAAAGGTAAAATCAACAAACACAAACATGCTAATGGTATTAAGATCGGAACCAGCCATTCATGTGTTCTTTGCAGCAAGGCTTTTTCATCGAAAAAAGCTTTGAATGGACACATGAAATGGCACAGCCAAAATGATTCGAAGAAGGGTAGTAACAATTACGACCTAGATCAGTTTCCATCCATTGATCTGACAAAGTATTTGCCACCAATAAGATACGAGACCAAGAAACGGAGCTGGGATTATATTCTTGATGTTGAAACTGTTAACATTGCTGAAATAATGGTGGATATGTCTCAGCTATCATCTGCCAATCTTAATGTGAATCACCGTGACTGTAAAAGGATGAAACTTTGCAGCAATGTTGATGATGATGATGTGAAGATCAATGAACAAGGTGGTAAAAACAATGAAAAGAACAGGTTGGTGGTGAGATTAAAGATTCCAAAAGGACTTATTACTCAAACATCTCAAGCTAGCAAGCAACCTGCCTCCAATGATAACAATGTAGAGAACAATACCGATGAAGGTGATGAAACGGATGGGGAATCAACTCTTGAATTAGAGTCACACATTTTTCGGGATTTTGATTTGAATGAGCTACTGGAAGATGATGTTGTAGATGAAACCACCATTTAG

Protein sequence

>MsaT002455.1

MMKNSFSSSSPSSHNSIHHKQKSNVVDHENEKHQQGARLHKCDLCAREFTTGNALGGHKQFHSTNNFLKGKINKHKHANGIKIGTSHSCVLCSKAFSSKKALNGHMKWHSQNDSKKGSNNYDLDQFPSIDLTKYLPPIRYETKKRSWDYILDVETVNIAEIMVDMSQLSSANLNVNHRDCKRMKLCSNVDDDDVKINEQGGKNNEKNRLVVRLKIPKGLITQTSQASKQPASNDNNVENNTDEGDETDGESTLELESHIFRDFDLNELLEDDVVDETTI*