Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG002455 | KEH41670.1 | 71.600 | 250 | 43 | 4 | 20 | 260 | 16 | 246 | 1.74e-113 | 337 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG002455 | tr|A0A072VU80|A0A072VU80_MEDTR | 71.600 | 250 | 43 | 4 | 20 | 260 | 16 | 246 | 8.31e-114 | 337 |
Gene ID | Type | Classification |
---|---|---|
MsaG002455 | TF | C2H2 |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000680 | MsaG002455 | 0.821026 | 8.823323e-53 | 1.994043e-50 |
MsaG001208 | MsaG002455 | 0.861375 | 2.116708e-63 | 1.734107e-60 |
MsaG001328 | MsaG002455 | 0.844807 | 1.126551e-58 | 5.129725e-56 |
MsaG002495 | MsaG002455 | 0.821892 | 5.579551e-53 | 1.290191e-50 |
MsaG002620 | MsaG002455 | 0.807464 | 8.479319e-50 | 1.362255e-47 |
MsaG002966 | MsaG002455 | 0.835047 | 3.836676e-56 | 1.286974e-53 |
MsaG003289 | MsaG002455 | 0.822200 | 4.737958e-53 | 1.104650e-50 |
MsaG003444 | MsaG002455 | 0.824881 | 1.125345e-53 | 2.821624e-51 |
MsaG003715 | MsaG002455 | 0.803397 | 5.984860e-49 | 8.742404e-47 |
MsaG004441 | MsaG002455 | 0.883072 | 1.305390e-70 | 2.724135e-67 |
MsaG004757 | MsaG002455 | 0.830784 | 4.350691e-55 | 1.287677e-52 |
MsaG005214 | MsaG002455 | 0.807813 | 7.155698e-50 | 1.159279e-47 |
MsaG006122 | MsaG002455 | 0.813965 | 3.380697e-51 | 6.367426e-49 |
MsaG007149 | MsaG002455 | 0.820043 | 1.479385e-52 | 3.257529e-50 |
MsaG008515 | MsaG002455 | 0.808512 | 5.087552e-50 | 8.381824e-48 |
MsaG010252 | MsaG002455 | 0.876320 | 3.191993e-68 | 4.868961e-65 |
MsaG011050 | MsaG002455 | 0.809707 | 2.828748e-50 | 4.796667e-48 |
MsaG011098 | MsaG002455 | 0.853223 | 5.293106e-61 | 3.209786e-58 |
MsaG012389 | MsaG002455 | 0.822657 | 3.714126e-53 | 8.766778e-51 |
MsaG012633 | MsaG002455 | 0.812671 | 6.484845e-51 | 1.182452e-48 |
MsaG041919 | MsaG002455 | 0.807839 | 7.067180e-50 | 1.145626e-47 |
MsaG042071 | MsaG002455 | 0.813517 | 4.239133e-51 | 7.895029e-49 |
MsaG043171 | MsaG002455 | 0.807396 | 8.765580e-50 | 1.405932e-47 |
MsaG045952 | MsaG002455 | 0.869186 | 7.577810e-66 | 8.486765e-63 |
MsaG046614 | MsaG002455 | 0.808947 | 4.110055e-50 | 6.842932e-48 |
MsaG047119 | MsaG002455 | 0.827982 | 2.070233e-54 | 5.658410e-52 |
MsaG047139 | MsaG002455 | 0.829557 | 8.646010e-55 | 2.470702e-52 |
MsaG000818 | MsaG002455 | 0.878941 | 3.925626e-69 | 6.747809e-66 |
MsaG002455 | MsaG002323 | 0.813496 | 4.283022e-51 | 7.972350e-49 |
MsaG002455 | MsaG001949 | 0.818093 | 4.090124e-52 | 8.557448e-50 |
MsaG002455 | MsaG008516 | 0.814524 | 2.548504e-51 | 4.867594e-49 |
MsaG002455 | MsaG008811 | 0.812178 | 8.300758e-51 | 1.495130e-48 |
MsaG002455 | MsaG008232 | 0.806767 | 1.189400e-49 | 1.879583e-47 |
MsaG002455 | MsaG023010 | 0.867821 | 2.081470e-65 | 2.204186e-62 |
MsaG002455 | MsaG026065 | 0.803742 | 5.079950e-49 | 7.480175e-47 |
MsaG002455 | MsaG028049 | 0.807603 | 7.924091e-50 | 1.277274e-47 |
MsaG002455 | MsaG033146 | 0.807957 | 6.669576e-50 | 1.084269e-47 |
MsaG002455 | MsaG031186 | 0.818538 | 3.246729e-52 | 6.871460e-50 |
MsaG002455 | MsaG032445 | 0.822416 | 4.223916e-53 | 9.905529e-51 |
MsaG002455 | MsaG032718 | 0.810493 | 1.919190e-50 | 3.317111e-48 |
MsaG002455 | MsaG030836 | 0.838007 | 6.820690e-57 | 2.502061e-54 |
MsaG002455 | MsaG036754 | 0.885993 | 1.088404e-71 | 2.617715e-68 |
MsaG002455 | MsaG037511 | 0.803655 | 5.293486e-49 | 7.778852e-47 |
MsaG002455 | MsaG038026 | 0.806520 | 1.340092e-49 | 2.105430e-47 |
MsaG002455 | MsaG039898 | 0.804893 | 2.932594e-49 | 4.434942e-47 |
MsaG002455 | MsaG040780 | 0.800720 | 2.112066e-48 | 2.902542e-46 |
MsaG002455 | MsaG042416 | 0.819452 | 2.016315e-52 | 4.370969e-50 |
MsaG002455 | MsaG042876 | 0.862347 | 1.070252e-63 | 9.104084e-61 |
MsaG012671 | MsaG002455 | 0.808597 | 4.878895e-50 | 8.054559e-48 |
MsaG013280 | MsaG002455 | 0.807844 | 7.049606e-50 | 1.142930e-47 |
MsaG013779 | MsaG002455 | 0.817156 | 6.635353e-52 | 1.355211e-49 |
MsaG014451 | MsaG002455 | 0.801201 | 1.686683e-48 | 2.343099e-46 |
MsaG016772 | MsaG002455 | 0.802960 | 7.362597e-49 | 1.064690e-46 |
MsaG016980 | MsaG002455 | 0.857021 | 4.216357e-62 | 2.931608e-59 |
MsaG017035 | MsaG002455 | 0.846344 | 4.336225e-59 | 2.077339e-56 |
MsaG017311 | MsaG002455 | 0.851383 | 1.757207e-60 | 9.988688e-58 |
MsaG017382 | MsaG002455 | 0.803903 | 4.705295e-49 | 6.954166e-47 |
MsaG017773 | MsaG002455 | 0.837513 | 9.123602e-57 | 3.296714e-54 |
MsaG018269 | MsaG002455 | 0.828080 | 1.960881e-54 | 5.374209e-52 |
MsaG019105 | MsaG002455 | 0.801308 | 1.603896e-48 | 2.233535e-46 |
MsaG019351 | MsaG002455 | 0.831780 | 2.483252e-55 | 7.565890e-53 |
MsaG020130 | MsaG002455 | 0.805791 | 1.905336e-49 | 2.942276e-47 |
MsaG020161 | MsaG002455 | 0.844815 | 1.121228e-58 | 5.106750e-56 |
MsaG020747 | MsaG002455 | 0.818964 | 2.600992e-52 | 5.566651e-50 |
MsaG023535 | MsaG002455 | 0.801625 | 1.382187e-48 | 1.938701e-46 |
MsaG023626 | MsaG002455 | 0.808761 | 4.503497e-50 | 7.464388e-48 |
MsaG023588 | MsaG002455 | 0.825144 | 9.759823e-54 | 2.464788e-51 |
MsaG023816 | MsaG002455 | 0.806341 | 1.461060e-49 | 2.285829e-47 |
MsaG024453 | MsaG002455 | 0.811460 | 1.187568e-50 | 2.101692e-48 |
MsaG026903 | MsaG002455 | 0.853168 | 5.487501e-61 | 3.321151e-58 |
MsaG027055 | MsaG002455 | 0.802803 | 7.931146e-49 | 1.142757e-46 |
MsaG027863 | MsaG002455 | 0.834177 | 6.332926e-56 | 2.069653e-53 |
MsaG028161 | MsaG002455 | 0.832732 | 1.446437e-55 | 4.530555e-53 |
MsaG028388 | MsaG002455 | 0.866367 | 6.028018e-65 | 6.015664e-62 |
MsaG029421 | MsaG002455 | 0.806147 | 1.604627e-49 | 2.498833e-47 |
MsaG031124 | MsaG002455 | 0.826953 | 3.643497e-54 | 9.675686e-52 |
MsaG031248 | MsaG002455 | 0.812545 | 6.909216e-51 | 1.255919e-48 |
MsaG033214 | MsaG002455 | 0.820972 | 9.076664e-53 | 2.048364e-50 |
MsaG033771 | MsaG002455 | 0.829673 | 8.103546e-55 | 2.323339e-52 |
MsaG034047 | MsaG002455 | 0.821295 | 7.652559e-53 | 1.741759e-50 |
MsaG039468 | MsaG002455 | 0.814248 | 2.930862e-51 | 5.559351e-49 |
MsaG040733 | MsaG002455 | 0.836028 | 2.172569e-56 | 7.504881e-54 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG002455 | MtrunA17_Chr1g0174861 | 71.600 | 250 | 43 | 4 | 20 | 260 | 16 | 246 | 1.60e-117 | 337 |
MsaG002455 | MtrunA17_Chr1g0174871 | 60.364 | 275 | 84 | 7 | 20 | 278 | 20 | 285 | 1.40e-92 | 275 |
MsaG002455 | MtrunA17_Chr1g0169521 | 36.486 | 296 | 131 | 9 | 29 | 277 | 30 | 315 | 1.22e-41 | 145 |
MsaG002455 | MtrunA17_Chr1g0169561 | 34.576 | 295 | 136 | 8 | 29 | 276 | 30 | 314 | 1.83e-38 | 137 |
MsaG002455 | MtrunA17_Chr1g0174001 | 39.500 | 200 | 87 | 7 | 106 | 277 | 1 | 194 | 3.02e-27 | 104 |
MsaG002455 | MtrunA17_Chr1g0172961 | 32.636 | 239 | 129 | 9 | 29 | 238 | 23 | 258 | 5.39e-25 | 100 |
MsaG002455 | MtrunA17_Chr1g0172861 | 31.868 | 273 | 129 | 13 | 29 | 268 | 23 | 271 | 1.27e-23 | 97.1 |
MsaG002455 | MtrunA17_Chr7g0214511 | 33.600 | 250 | 111 | 10 | 49 | 277 | 71 | 286 | 1.10e-22 | 94.7 |
MsaG002455 | MtrunA17_Chr4g0037041 | 41.270 | 126 | 57 | 5 | 28 | 144 | 22 | 139 | 5.92e-19 | 82.4 |
MsaG002455 | MtrunA17_Chr5g0448221 | 34.177 | 158 | 73 | 8 | 33 | 166 | 54 | 204 | 1.60e-11 | 63.2 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 50 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TTCTTGGTCTCGTATCTTAT+TGG | 0.208428 | 1:+41595647 | None:intergenic |
AGCACCAACAACTTTCTTAA+AGG | 0.236987 | 1:-41595869 | MsaT002455.1:CDS |
TACCCTTCTTCGAATCATTT+TGG | 0.242767 | 1:+41595726 | None:intergenic |
AAACAAAATTAAACACTAAA+TGG | 0.270394 | 1:+41595223 | None:intergenic |
ATAATCCCAGCTCCGTTTCT+TGG | 0.287583 | 1:+41595631 | None:intergenic |
GTTCTCTACATTGTTATCAT+TGG | 0.335954 | 1:+41595361 | None:intergenic |
GTTTGAGTAATAAGTCCTTT+TGG | 0.343516 | 1:+41595410 | None:intergenic |
TGAATCACCGTGACTGTAAA+AGG | 0.346155 | 1:-41595532 | MsaT002455.1:CDS |
TGTTAACATTGCTGAAATAA+TGG | 0.391363 | 1:-41595591 | MsaT002455.1:CDS |
TTTACCTTTAAGAAAGTTGT+TGG | 0.419418 | 1:+41595865 | None:intergenic |
AACAAACACAAACATGCTAA+TGG | 0.425038 | 1:-41595839 | MsaT002455.1:CDS |
TAAGAAAGTTGTTGGTGCTA+TGG | 0.440297 | 1:+41595873 | None:intergenic |
CTCTACATTGTTATCATTGG+AGG | 0.445625 | 1:+41595364 | None:intergenic |
CTTTGAATGGACACATGAAA+TGG | 0.459517 | 1:-41595754 | MsaT002455.1:CDS |
TTGGTCTCGTATCTTATTGG+TGG | 0.473127 | 1:+41595650 | None:intergenic |
ACGGTGATTCACATTAAGAT+TGG | 0.473501 | 1:+41595544 | None:intergenic |
CAATGGATGGAAACTGATCT+AGG | 0.474117 | 1:+41595690 | None:intergenic |
ATCCGTTTCATCACCTTCAT+CGG | 0.483860 | 1:+41595334 | None:intergenic |
TGGTGCTATGGAATTGTTTG+TGG | 0.484496 | 1:+41595885 | None:intergenic |
TGTGCTAGAGAATTCACAAC+TGG | 0.492819 | 1:-41595923 | MsaT002455.1:CDS |
CAAATACTTTGTCAGATCAA+TGG | 0.494617 | 1:+41595673 | None:intergenic |
GAATTGTTTGTGGCCGCCGA+GGG | 0.497780 | 1:+41595895 | None:intergenic |
TGAATAGAATTATGAGAAGA+AGG | 0.504138 | 1:+41596025 | None:intergenic |
TTCATGTGTTCTTTGCAGCA+AGG | 0.509354 | 1:-41595795 | MsaT002455.1:CDS |
CAGCCAAAATGATTCGAAGA+AGG | 0.519454 | 1:-41595729 | MsaT002455.1:CDS |
TACCGATGAAGGTGATGAAA+CGG | 0.519602 | 1:-41595336 | MsaT002455.1:CDS |
CATGCTAATGGTATTAAGAT+CGG | 0.524324 | 1:-41595827 | MsaT002455.1:CDS |
GTGAGATTAAAGATTCCAAA+AGG | 0.530313 | 1:-41595425 | MsaT002455.1:CDS |
ACATTGTTATCATTGGAGGC+AGG | 0.530674 | 1:+41595368 | None:intergenic |
ACGAGACCAAGAAACGGAGC+TGG | 0.539882 | 1:-41595637 | MsaT002455.1:CDS |
GGAATTGTTTGTGGCCGCCG+AGG | 0.556033 | 1:+41595894 | None:intergenic |
AGCCAAAATGATTCGAAGAA+GGG | 0.558347 | 1:-41595728 | MsaT002455.1:CDS |
TTCACAACTGGTAATGCCCT+CGG | 0.558819 | 1:-41595911 | MsaT002455.1:CDS |
ATGAAGGTGATGAAACGGAT+GGG | 0.561647 | 1:-41595331 | MsaT002455.1:CDS |
GCAAAGAACACATGAATGGC+TGG | 0.562308 | 1:+41595802 | None:intergenic |
GTAGAGAACAATACCGATGA+AGG | 0.568109 | 1:-41595347 | MsaT002455.1:CDS |
CGAGACCAAGAAACGGAGCT+GGG | 0.594941 | 1:-41595636 | MsaT002455.1:CDS |
CAATGAAAAGAACAGGTTGG+TGG | 0.599420 | 1:-41595447 | MsaT002455.1:CDS |
CAAAATTAAACACTAAATGG+TGG | 0.600626 | 1:+41595226 | None:intergenic |
TACTTTGTCAGATCAATGGA+TGG | 0.603001 | 1:+41595677 | None:intergenic |
TGAAGGTGATGAAACGGATG+GGG | 0.603593 | 1:-41595330 | MsaT002455.1:CDS |
TAAGATACGAGACCAAGAAA+CGG | 0.610128 | 1:-41595643 | MsaT002455.1:CDS |
GATGAAGGTGATGAAACGGA+TGG | 0.616100 | 1:-41595332 | MsaT002455.1:CDS |
AAACAATGAAAAGAACAGGT+TGG | 0.620052 | 1:-41595450 | MsaT002455.1:CDS |
TGCTGCAAAGAACACATGAA+TGG | 0.640126 | 1:+41595798 | None:intergenic |
TAACATTGCTGAAATAATGG+TGG | 0.655107 | 1:-41595588 | MsaT002455.1:CDS |
ACAACTGGTAATGCCCTCGG+CGG | 0.704742 | 1:-41595908 | MsaT002455.1:CDS |
GAAAATGAGAAACATCAACA+AGG | 0.726705 | 1:-41595971 | MsaT002455.1:CDS |
GATGTGAAGATCAATGAACA+AGG | 0.760498 | 1:-41595479 | MsaT002455.1:CDS |
GTGAAGATCAATGAACAAGG+TGG | 0.773369 | 1:-41595476 | MsaT002455.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr1 | gene | 41595238 | 41596077 | 41595238 | ID=MsaG002455 |
Chr1 | mRNA | 41595238 | 41596077 | 41595238 | ID=MsaT002455.1;Parent=MsaG002455 |
Chr1 | exon | 41595238 | 41596077 | 41595238 | ID=MsaT002455.1.exon1;Parent=MsaT002455.1 |
Chr1 | CDS | 41595238 | 41596077 | 41595238 | ID=cds.MsaT002455.1;Parent=MsaT002455.1 |
Gene Sequence |
Protein sequence |