AlfalfaGEDB | MsaG002456

M. sativa ssp. caerulea / MsaG002456

KEGG: -
GO: -
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002456	RHN79225.1	75.000	316	46	4	1	313	1	286	1.85e-137	400

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002456	tr\|A0A396JSM8\|A0A396JSM8_MEDTR	75.000	316	46	4	1	313	1	286	8.83e-138	400

TFs/TRs:

Gene ID	Type	Classification
MsaG002456	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000157	MsaG002456	0.805631	2.057353e-49	3.165245e-47
MsaG001357	MsaG002456	0.824619	1.296728e-53	3.228052e-51
MsaG001413	MsaG002456	0.820574	1.119326e-52	2.499484e-50
MsaG002292	MsaG002456	0.817556	5.400191e-52	1.114328e-49
MsaG003793	MsaG002456	0.806352	1.453396e-49	2.274427e-47
MsaG003946	MsaG002456	0.855799	9.594170e-62	6.379713e-59
MsaG005401	MsaG002456	0.822994	3.103265e-53	7.391974e-51
MsaG005508	MsaG002456	0.814969	2.032244e-51	3.925548e-49
MsaG005705	MsaG002456	0.810121	2.306701e-50	3.950803e-48
MsaG006023	MsaG002456	0.813471	4.338374e-51	8.070452e-49
MsaG006084	MsaG002456	0.803965	4.568434e-49	6.761796e-47
MsaG006175	MsaG002456	0.836020	2.183587e-56	7.541194e-54
MsaG006561	MsaG002456	0.827032	3.489710e-54	9.287278e-52
MsaG006867	MsaG002456	0.825278	9.077246e-54	2.300873e-51
MsaG007328	MsaG002456	0.835885	2.360749e-56	8.120199e-54
MsaG007716	MsaG002456	0.846523	3.876847e-59	1.868325e-56
MsaG010533	MsaG002456	0.848704	9.803872e-60	5.083719e-57
MsaG010835	MsaG002456	0.816685	8.455968e-52	1.706158e-49
MsaG010900	MsaG002456	0.830853	4.184962e-55	1.241123e-52
MsaG011974	MsaG002456	0.816253	1.055315e-51	2.105924e-49
MsaG012418	MsaG002456	0.843341	2.774590e-58	1.204548e-55
MsaG041899	MsaG002456	0.808459	5.221661e-50	8.591813e-48
MsaG041901	MsaG002456	0.801152	1.725396e-48	2.394297e-46
MsaG045452	MsaG002456	0.824216	1.610865e-53	3.966295e-51
MsaG045487	MsaG002456	0.833437	9.677927e-56	3.094706e-53
MsaG046383	MsaG002456	0.811725	1.040925e-50	1.854151e-48
MsaG046512	MsaG002456	0.864570	2.205941e-64	2.048607e-61
MsaG002331	MsaG002456	0.811218	1.339502e-50	2.356555e-48
MsaG002456	MsaG009542	0.819537	1.928663e-52	4.190049e-50
MsaG002456	MsaG014346	0.800112	2.806113e-48	3.803576e-46
MsaG002456	MsaG019142	0.801825	1.258152e-48	1.772808e-46
MsaG002456	MsaG022111	0.802542	8.973858e-49	1.285334e-46
MsaG002456	MsaG030315	0.801629	1.379280e-48	1.934834e-46
MsaG002456	MsaG033321	0.801789	1.279505e-48	1.801388e-46
MsaG013371	MsaG002456	0.835996	2.213893e-56	7.640299e-54
MsaG013845	MsaG002456	0.803851	4.822271e-49	7.118559e-47
MsaG017058	MsaG002456	0.857937	2.264766e-62	1.629313e-59
MsaG017173	MsaG002456	0.823818	1.995887e-53	4.861635e-51
MsaG017820	MsaG002456	0.809903	2.568880e-50	4.376686e-48
MsaG018248	MsaG002456	0.802757	8.102836e-49	1.166293e-46
MsaG019180	MsaG002456	0.819890	1.603082e-52	3.515473e-50
MsaG019382	MsaG002456	0.824448	1.421876e-53	3.523262e-51
MsaG019410	MsaG002456	0.827423	2.814884e-54	7.574023e-52
MsaG019925	MsaG002456	0.840435	1.611711e-57	6.378153e-55
MsaG020143	MsaG002456	0.800516	2.324093e-48	3.179114e-46
MsaG020540	MsaG002456	0.808447	5.250395e-50	8.636693e-48
MsaG020580	MsaG002456	0.807140	9.923459e-50	1.582011e-47
MsaG021043	MsaG002456	0.800907	1.935583e-48	2.671096e-46
MsaG021668	MsaG002456	0.801144	1.732233e-48	2.403322e-46
MsaG021863	MsaG002456	0.808218	5.873885e-50	9.609022e-48
MsaG023971	MsaG002456	0.808706	4.626399e-50	7.658114e-48
MsaG023986	MsaG002456	0.859099	1.023901e-62	7.693032e-60
MsaG025484	MsaG002456	0.811881	9.630214e-51	1.722006e-48
MsaG028034	MsaG002456	0.807457	8.510460e-50	1.367014e-47
MsaG028444	MsaG002456	0.876621	2.514901e-68	3.887944e-65
MsaG028715	MsaG002456	0.811566	1.126464e-50	1.998750e-48
MsaG034827	MsaG002456	0.807003	1.060525e-49	1.685263e-47
MsaG034829	MsaG002456	0.802994	7.244286e-49	1.048429e-46
MsaG035148	MsaG002456	0.817499	5.559235e-52	1.145480e-49
MsaG037719	MsaG002456	0.869131	7.896869e-66	8.824240e-63
MsaG038016	MsaG002456	0.806302	1.488790e-49	2.327088e-47
MsaG040072	MsaG002456	0.838351	5.569666e-57	2.064922e-54
MsaG040324	MsaG002456	0.811721	1.042785e-50	1.857298e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002456	MtrunA17_Chr1g0174871	75.000	316	46	4	1	313	1	286	1.70e-141	400
MsaG002456	MtrunA17_Chr1g0174861	54.251	247	82	9	17	258	16	236	1.70e-67	211
MsaG002456	MtrunA17_Chr1g0169521	31.498	327	135	12	26	311	37	315	3.76e-30	116
MsaG002456	MtrunA17_Chr1g0169561	31.402	328	132	12	26	310	37	314	3.59e-28	110
MsaG002456	MtrunA17_Chr1g0172861	30.189	318	159	15	5	309	11	278	2.51e-19	85.9
MsaG002456	MtrunA17_Chr1g0172961	31.330	233	134	9	27	241	30	254	1.31e-17	81.3
MsaG002456	MtrunA17_Chr7g0214511	30.108	279	126	11	39	311	71	286	6.37e-17	79.3
MsaG002456	MtrunA17_Chr4g0037041	38.298	141	66	5	5	136	11	139	8.87e-16	74.3
MsaG002456	MtrunA17_Chr1g0174001	32.051	234	101	10	96	311	1	194	6.67e-15	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 61 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATCTGTCAATGCTCTAAA+TGG	0.239919	1:-41601173	MsaT002456.1:CDS
ATAATTCCAGCTCCGTTTCT+TGG	0.269877	1:+41601031	None:intergenic
GAAGAAAGTTGTTGCCTCTA+TGG	0.295075	1:+41601279	None:intergenic
AGAGGCAACAACTTTCTTCT+AGG	0.323367	1:-41601275	MsaT002456.1:CDS
TCATCTTCACCAGGGTTGTT+TGG	0.341135	1:+41600618	None:intergenic
TGAGGAACCAACTCATGAAT+TGG	0.350528	1:-41600586	MsaT002456.1:CDS
ACTCTCATCACAAACCAAAA+AGG	0.357704	1:-41601394	MsaT002456.1:CDS
GTTCTCTTCATTATCATCAT+TGG	0.362989	1:+41600716	None:intergenic
TCTTCGAAGTCGAACCATAT+TGG	0.367921	1:+41601132	None:intergenic
ATGAAATCGCACAGCCAATA+TGG	0.368027	1:-41601146	MsaT002456.1:CDS
TCATCTTCACCAGTGTTGTT+TGG	0.374435	1:+41600657	None:intergenic
ATCATTGGAAGCAGATTGAT+TGG	0.381296	1:+41600731	None:intergenic
GAGGAACCAACTCATGAATT+GGG	0.382420	1:-41600585	MsaT002456.1:CDS
AAATACTTTCTCAAATCAAT+TGG	0.400225	1:+41601074	None:intergenic
TGATTCCCAACAAGGTTGTT+AGG	0.405927	1:+41600951	None:intergenic
TTCTTGGTCACATACCTCAT+TGG	0.415556	1:+41601047	None:intergenic
TGCAGAAATGTGGGTGGGAA+TGG	0.425504	1:-41600903	MsaT002456.1:CDS
CGATCACCTAACAACCTTGT+TGG	0.429642	1:-41600957	MsaT002456.1:CDS
TGCCTCTATGGAATGCTTTG+TGG	0.438332	1:+41601291	None:intergenic
GATCACCTAACAACCTTGTT+GGG	0.460149	1:-41600956	MsaT002456.1:CDS
TACTTTCTCAAATCAATTGG+TGG	0.486384	1:+41601077	None:intergenic
TCATGTTGCTGAAGCAATGC+TGG	0.487886	1:-41600991	MsaT002456.1:CDS
GCAGAAATGTGGGTGGGAAT+GGG	0.488317	1:-41600902	MsaT002456.1:CDS
TGTGACAGAGAATTCACAAC+TGG	0.492142	1:-41601329	MsaT002456.1:CDS
GAATGCTTTGTGGCCGCCGA+GGG	0.493083	1:+41601301	None:intergenic
TTTGCTTCATCATCTTCACC+AGG	0.497903	1:+41600609	None:intergenic
TTTAACCCCAATTCATGAGT+TGG	0.505846	1:+41600579	None:intergenic
CAATTGGTGGAAATTGATCC+AGG	0.509751	1:+41601090	None:intergenic
GGAATCACCGTGTCACGAAA+AGG	0.512728	1:-41600935	MsaT002456.1:CDS
TTGGTCACATACCTCATTGG+TGG	0.513533	1:+41601050	None:intergenic
GATCAAACTTTGCAGAAATG+TGG	0.524856	1:-41600913	MsaT002456.1:CDS
ATATGGTTCGACTTCGAAGA+AGG	0.528018	1:-41601129	MsaT002456.1:CDS
ATCAAACTTTGCAGAAATGT+GGG	0.554714	1:-41600912	MsaT002456.1:CDS
AAAAGGAGTGTTGTTGATCA+TGG	0.562216	1:-41601377	MsaT002456.1:CDS
TTCACAACTGGTAACGCCCT+CGG	0.563147	1:-41601317	MsaT002456.1:CDS
TGAGGTATGTGACCAAGAAA+CGG	0.563267	1:-41601043	MsaT002456.1:CDS
AAACAATGAAAAGAACAAGT+TGG	0.564169	1:-41600805	MsaT002456.1:CDS
GGGTAGTAAGATGTATGACC+TGG	0.568308	1:-41601108	MsaT002456.1:CDS
ATGTGACCAAGAAACGGAGC+TGG	0.575391	1:-41601037	MsaT002456.1:CDS
TCATCTGCATCATCATCCAC+TGG	0.575486	1:+41600525	None:intergenic
AACTTTGCAGAAATGTGGGT+GGG	0.585412	1:-41600908	MsaT002456.1:CDS
GGAATGCTTTGTGGCCGCCG+AGG	0.588007	1:+41601300	None:intergenic
TGAAGATGATGAAGCAAATG+AGG	0.593094	1:-41600604	MsaT002456.1:CDS
AAAAGAACAAGTTGGTGGTG+AGG	0.597114	1:-41600797	MsaT002456.1:CDS
CGATTTGAATGAGCTGCCAG+TGG	0.597243	1:-41600541	MsaT002456.1:CDS
AAGATGAAGATCAGAGAGCA+AGG	0.598147	1:-41600879	MsaT002456.1:CDS
TATGGTTCGACTTCGAAGAA+GGG	0.600043	1:-41601128	MsaT002456.1:CDS
TTGCTTCATCATCTTCACCA+GGG	0.604583	1:+41600610	None:intergenic
TGACACGGTGATTCCCAACA+AGG	0.616814	1:+41600943	None:intergenic
CAATGAAAAGAACAAGTTGG+TGG	0.617009	1:-41600802	MsaT002456.1:CDS
AAACTTTGCAGAAATGTGGG+TGG	0.628923	1:-41600909	MsaT002456.1:CDS
AGGAACCAACTCATGAATTG+GGG	0.629510	1:-41600584	MsaT002456.1:CDS
GAAAGTATTTGCCACCAATG+AGG	0.638748	1:-41601061	MsaT002456.1:CDS
GGCCACAAAGCATTCCATAG+AGG	0.645608	1:-41601293	MsaT002456.1:CDS
TGAAGATGATGAAGCAGACG+AGG	0.647378	1:-41600682	MsaT002456.1:CDS
TGCTGCAACGAACACATGAA+TGG	0.655011	1:+41601204	None:intergenic
GACGAGGAACCAAACAACAC+TGG	0.659138	1:-41600666	MsaT002456.1:CDS
TGAAGATGATGAAGCAGATG+AGG	0.659995	1:-41600643	MsaT002456.1:CDS
AATGAAGAGAACAACAACAC+TGG	0.663304	1:-41600705	MsaT002456.1:CDS
GATGAGGAACCAAACAACCC+TGG	0.684317	1:-41600627	MsaT002456.1:CDS
ACAACTGGTAACGCCCTCGG+CGG	0.717230	1:-41601314	MsaT002456.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	41600512	41601453	41600512	ID=MsaG002456
Chr1	mRNA	41600512	41601453	41600512	ID=MsaT002456.1;Parent=MsaG002456
Chr1	exon	41600512	41601453	41600512	ID=MsaT002456.1.exon1;Parent=MsaT002456.1
Chr1	CDS	41600512	41601453	41600512	ID=cds.MsaT002456.1;Parent=MsaT002456.1

Gene Sequence

>MsaT002456.1

ATGAAGCATTTTTTTTCTTCTGCTTCTTCTTCATCTCACTCTCATCACAAACCAAAAAGGAGTGTTGTTGATCATGGTGCAAAACTTCACAAATGTGATTTGTGTGACAGAGAATTCACAACTGGTAACGCCCTCGGCGGCCACAAAGCATTCCATAGAGGCAACAACTTTCTTCTAGGAAAAATCAACAAAGAAAAACAAGCTGATGGTATTAAGATCGAAAACAACCATTCATGTGTTCGTTGCAGCAAGACTTTTTCATCTGTCAATGCTCTAAATGGACACATGAAATCGCACAGCCAATATGGTTCGACTTCGAAGAAGGGTAGTAAGATGTATGACCTGGATCAATTTCCACCAATTGATTTGAGAAAGTATTTGCCACCAATGAGGTATGTGACCAAGAAACGGAGCTGGAATTATGTTATTAATGTTGAAGCTCATGTTGCTGAAGCAATGCTGGATATGTCACAGCGATCACCTAACAACCTTGTTGGGAATCACCGTGTCACGAAAAGGATCAAACTTTGCAGAAATGTGGGTGGGAATGGGAAGATGAAGATCAGAGAGCAAGGTATTGTGATAAAACATTGTGTTGATGAAACAATTGTGAGAGAGAATGATAAAAACAATGAAAAGAACAAGTTGGTGGTGAGGTTAAAAATTCCGAATAGCCTTGTCATTCAAAAATCACAAGCTTCCAATCAATCTGCTTCCAATGATGATAATGAAGAGAACAACAACACTGGTGAAGATGATGAAGCAGACGAGGAACCAAACAACACTGGTGAAGATGATGAAGCAGATGAGGAACCAAACAACCCTGGTGAAGATGATGAAGCAAATGAGGAACCAACTCATGAATTGGGGTTAAATGTTGTTCGAGATTTCGATTTGAATGAGCTGCCAGTGGATGATGATGCAGATGAAACCGCCCATTAG

Protein sequence

>MsaT002456.1

MKHFFSSASSSSHSHHKPKRSVVDHGAKLHKCDLCDREFTTGNALGGHKAFHRGNNFLLGKINKEKQADGIKIENNHSCVRCSKTFSSVNALNGHMKSHSQYGSTSKKGSKMYDLDQFPPIDLRKYLPPMRYVTKKRSWNYVINVEAHVAEAMLDMSQRSPNNLVGNHRVTKRIKLCRNVGGNGKMKIREQGIVIKHCVDETIVRENDKNNEKNKLVVRLKIPNSLVIQKSQASNQSASNDDNEENNNTGEDDEADEEPNNTGEDDEADEEPNNPGEDDEANEEPTHELGLNVVRDFDLNELPVDDDADETAH*