AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002459


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002459 MtrunA17_Chr1g0174911 87.097 248 22 2 1 248 1 238 2.75e-153 429
MsaG002459 MtrunA17_Chr1g0168571 60.784 255 72 8 1 245 1 237 1.17e-88 265
MsaG002459 MtrunA17_Chr7g0274971 59.615 208 51 6 1 183 1 200 1.48e-76 231
MsaG002459 MtrunA17_Chr7g0214071 32.143 168 91 9 11 170 19 171 1.48e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGAATCCTTGGGATATT+TGG 0.040443 1:-41635315 MsaT002459.1:CDS
CCACCATTTGATTCGTTTCT+TGG 0.076491 1:+41634591 None:intergenic
TACAAATTTGTTGGCTTCTT+CGG 0.206819 1:+41634470 None:intergenic
AAATATCCCAAGGATTCTTT+AGG 0.262491 1:+41635317 None:intergenic
TCAAGAATGGCGATATAATT+TGG 0.272332 1:+41635358 None:intergenic
ACAAATTTGTTGGCTTCTTC+GGG 0.289766 1:+41634471 None:intergenic
AGTGGTAGATTTCTTCTCTA+AGG 0.296212 1:+41635231 None:intergenic
TCAAAAGTAGTTCGTTCTAT+TGG 0.321604 1:-41635205 MsaT002459.1:CDS
AAAGAATCCTTGGGATATTT+GGG 0.338135 1:-41635314 MsaT002459.1:CDS
TGGGATATTTGGGAAGAGTT+TGG 0.341365 1:-41635304 MsaT002459.1:CDS
AATTTGGCAGAGATTTGTTA+TGG 0.353680 1:+41635374 None:intergenic
TAATACAAAGTCTCATTATA+AGG 0.356324 1:-41634547 MsaT002459.1:CDS
GTAGTTCGTTCTATTGGAAT+TGG 0.365191 1:-41635199 MsaT002459.1:CDS
ATCTTTGATAAGAAATCCTT+TGG 0.365896 1:-41635029 MsaT002459.1:intron
TCTCAATTCTTCTTCAAGTA+AGG 0.366602 1:-41635452 MsaT002459.1:CDS
AATATCCCAAGGATTCTTTA+GGG 0.371252 1:+41635318 None:intergenic
CTAAGAATGGTTGTCTCTCT+TGG 0.381735 1:+41634495 None:intergenic
ATTCTTGAATACGATTTGTT+TGG 0.392427 1:-41635343 MsaT002459.1:CDS
TGGACACAATAGCACAAATA+TGG 0.393645 1:-41634571 MsaT002459.1:CDS
TGTAGGACTTACAAATTTGT+TGG 0.396611 1:+41634461 None:intergenic
TTAAGGTAGTGAAGAAATAT+AGG 0.411633 1:+41635254 None:intergenic
GGCTTCTTCGGGTCTAAGAA+TGG 0.413104 1:+41634482 None:intergenic
GAAAGAAAGACCATTGTTCC+TGG 0.417974 1:-41634868 MsaT002459.1:CDS
TTGGTACCCTAAAGAATCCT+TGG 0.424102 1:-41635324 MsaT002459.1:CDS
AATCAAGTAACTTACAGATA+TGG 0.426262 1:+41634831 None:intergenic
AGATTTCTTCTCTAAGGTTA+AGG 0.432842 1:+41635237 None:intergenic
TGGTACCCTAAAGAATCCTT+GGG 0.489046 1:-41635323 MsaT002459.1:CDS
GAACCAAGAAACGAATCAAA+TGG 0.503929 1:-41634594 MsaT002459.1:CDS
GTTCTATTGGAATTGGTGCA+TGG 0.508402 1:-41635192 MsaT002459.1:CDS
AAACAAATCGTATTCAAGAA+TGG 0.511320 1:+41635345 None:intergenic
TATTATACAACAACAAATGT+AGG 0.527932 1:+41634444 None:intergenic
TTCTTATCAAAGATTTGTCA+AGG 0.536486 1:+41635038 None:intergenic
ATGGTGGTTTCTGCACGTCC+AGG 0.545124 1:+41634850 None:intergenic
TTCTATTGGAATTGGTGCAT+GGG 0.546914 1:-41635191 MsaT002459.1:CDS
GCAAGATCCATGCACCATCA+TGG 0.566204 1:+41635071 None:intergenic
AACTCTTCCCAAATATCCCA+AGG 0.585203 1:+41635307 None:intergenic
CAAGTAACTTACAGATATGG+TGG 0.592493 1:+41634834 None:intergenic
TTCTGCACGTCCAGGAACAA+TGG 0.595867 1:+41634858 None:intergenic
ATTGGAATTGGTGCATGGGA+AGG 0.630255 1:-41635187 MsaT002459.1:CDS
GCACTTACCATGATGGTGCA+TGG 0.633065 1:-41635078 MsaT002459.1:CDS
AATAGTGGCACTTACCATGA+TGG 0.645072 1:-41635085 MsaT002459.1:CDS
AGAAAGAATATTGATGCACA+AGG 0.650579 1:-41634895 MsaT002459.1:CDS
TGGGAAGGTGAAGAAGACAC+TGG 0.669846 1:-41635172 MsaT002459.1:CDS
CCAAGAAACGAATCAAATGG+TGG 0.672770 1:-41634591 MsaT002459.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 41634446 41635479 41634446 ID=MsaG002459
Chr1 mRNA 41634446 41635479 41634446 ID=MsaT002459.1;Parent=MsaG002459
Chr1 exon 41634446 41634661 41634446 ID=MsaT002459.1.exon3;Parent=MsaT002459.1
Chr1 CDS 41634446 41634661 41634446 ID=cds.MsaT002459.1;Parent=MsaT002459.1
Chr1 exon 41634846 41634944 41634846 ID=MsaT002459.1.exon2;Parent=MsaT002459.1
Chr1 CDS 41634846 41634944 41634846 ID=cds.MsaT002459.1;Parent=MsaT002459.1
Chr1 exon 41635030 41635479 41635030 ID=MsaT002459.1.exon1;Parent=MsaT002459.1
Chr1 CDS 41635030 41635479 41635030 ID=cds.MsaT002459.1;Parent=MsaT002459.1
Gene Sequence

>MsaT002459.1

ATGGCTCTCAATTCTTCTTCAAGTAAGGATTTCAAATTCACACTCAGAGATGATGAACTTCTACGTTTCCTCTACAACAAAATCCATAACAAATCTCTGCCAAATTATATCGCCATTCTTGAATACGATTTGTTTGGTACCCTAAAGAATCCTTGGGATATTTGGGAAGAGTTTGGAGCTTCTCGCTCATATTGTGAAAAAGACCTATATTTCTTCACTACCTTAACCTTAGAGAAGAAATCTACCACTAGCTCAAAAGTAGTTCGTTCTATTGGAATTGGTGCATGGGAAGGTGAAGAAGACACTGGAAAAATTATAGTTGCAAAAAACAAGAACCAACTCCTTGGTGTGAAAAAGTGTTTTCGATTTGAGAATAGTGGCACTTACCATGATGGTGCATGGATCTTGCATGAATATAGCCTTGACAAATCTTTGATAAGAAATCCTTTGGCCAAAAATTATGTTCTATGCAGATTTAGAAAGAATATTGATGCACAAGGTCAAGAAAGAAAGACCATTGTTCCTGGACGTGCAGAAACCACCATATCTGGAAATAAAGATAAAGATTCGTTAACGAAATATATAATTTATAAAGAACCAAGAAACGAATCAAATGGTGGACACAATAGCACAAATATGGTTAATACAAAGTCTCATTATAAGGAAGATGAAAAAGTTGTGAGAAATATTTGGCCAAGAGAGACAACCATTCTTAGACCCGAAGAAGCCAACAAATTTGTAAGTCCTACATTTGTTGTTGTATAA

Protein sequence

>MsaT002459.1

MALNSSSSKDFKFTLRDDELLRFLYNKIHNKSLPNYIAILEYDLFGTLKNPWDIWEEFGASRSYCEKDLYFFTTLTLEKKSTTSSKVVRSIGIGAWEGEEDTGKIIVAKNKNQLLGVKKCFRFENSGTYHDGAWILHEYSLDKSLIRNPLAKNYVLCRFRKNIDAQGQERKTIVPGRAETTISGNKDKDSLTKYIIYKEPRNESNGGHNSTNMVNTKSHYKEDEKVVRNIWPRETTILRPEEANKFVSPTFVVV*