AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002467


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002467 MtrunA17_Chr1g0175071 91.304 161 14 0 3 163 160 320 6.42e-102 295
MsaG002467 MtrunA17_Chr7g0258531 60.432 139 50 2 20 156 213 348 1.45e-44 150
MsaG002467 MtrunA17_Chr1g0175091 52.593 135 63 1 16 150 254 387 1.75e-35 127
MsaG002467 MtrunA17_Chr7g0258551 50.758 132 65 0 19 150 211 342 3.72e-34 123
MsaG002467 MtrunA17_Chr1g0190301 45.139 144 78 1 8 150 163 306 7.54e-34 122
MsaG002467 MtrunA17_Chr7g0261911 47.445 137 69 2 16 150 258 393 1.93e-32 120
MsaG002467 MtrunA17_Chr2g0291021 47.445 137 63 2 20 150 49 182 1.39e-31 115
MsaG002467 MtrunA17_Chr7g0274471 43.939 132 73 1 20 150 218 349 8.54e-28 107
MsaG002467 MtrunA17_Chr7g0254981 38.889 144 76 2 15 150 45 184 3.28e-27 103
MsaG002467 MtrunA17_Chr2g0321461 41.509 159 72 6 9 146 34 192 4.86e-25 99.0
MsaG002467 MtrunA17_Chr8g0356501 37.975 158 86 2 9 158 26 179 1.20e-24 97.4
MsaG002467 MtrunA17_Chr2g0310011 40.141 142 73 2 9 142 24 161 1.53e-24 97.1
MsaG002467 MtrunA17_Chr6g0461641 38.217 157 72 3 15 150 37 189 1.54e-24 97.8
MsaG002467 MtrunA17_Chr5g0417521 39.041 146 76 3 13 145 8 153 9.32e-23 92.4
MsaG002467 MtrunA17_Chr4g0042451 37.931 145 74 3 15 145 12 154 2.25e-22 91.3
MsaG002467 MtrunA17_Chr5g0409731 36.943 157 80 4 20 160 25 178 4.61e-22 89.7
MsaG002467 MtrunA17_Chr7g0242521 42.308 130 70 3 16 141 9 137 1.02e-19 82.4
MsaG002467 MtrunA17_Chr2g0290991 34.266 143 81 3 15 144 34 176 2.32e-18 80.1
MsaG002467 MtrunA17_Chr1g0197171 34.351 131 84 2 9 138 33 162 1.78e-17 77.4
MsaG002467 MtrunA17_Chr1g0162931 62.264 53 20 0 22 74 20 72 7.12e-16 72.8
MsaG002467 MtrunA17_Chr1g0175081 55.172 58 26 0 16 73 18 75 2.35e-15 67.8
MsaG002467 MtrunA17_Chr8g0379121 51.429 70 26 1 22 91 113 174 2.70e-15 72.0
MsaG002467 MtrunA17_Chr1g0210911 41.304 92 40 2 22 101 24 113 3.20e-15 71.6
MsaG002467 MtrunA17_Chr2g0320231 51.429 70 26 1 22 91 99 160 1.48e-14 70.1
MsaG002467 MtrunA17_Chr3g0095611 60.377 53 21 0 22 74 21 73 1.81e-14 68.2
MsaG002467 MtrunA17_Chr7g0243081 56.604 53 23 0 22 74 73 125 9.85e-14 67.4
MsaG002467 MtrunA17_Chr6g0479431 47.059 68 34 1 22 89 204 269 1.70e-13 67.0
MsaG002467 MtrunA17_Chr1g0154261 49.206 63 32 0 22 84 128 190 1.97e-13 66.2
MsaG002467 MtrunA17_Chr3g0109981 38.532 109 51 3 22 130 276 368 2.85e-13 66.2
MsaG002467 MtrunA17_Chr2g0288601 57.143 49 21 0 22 70 73 121 4.37e-13 62.8
MsaG002467 MtrunA17_Chr8g0366281 54.717 53 24 0 22 74 175 227 4.80e-13 65.5
MsaG002467 MtrunA17_Chr2g0283951 50.000 74 25 2 22 91 91 156 7.07e-13 65.1
MsaG002467 MtrunA17_Chr5g0410741 52.830 53 25 0 22 74 221 273 1.32e-12 64.3
MsaG002467 MtrunA17_Chr7g0275401 50.877 57 27 1 20 76 192 247 3.09e-12 62.8
MsaG002467 MtrunA17_Chr4g0009901 53.704 54 24 1 20 73 168 220 1.42e-11 60.8
MsaG002467 MtrunA17_Chr2g0324891 48.529 68 32 2 9 74 148 214 4.73e-11 60.1
MsaG002467 MtrunA17_Chr1g0198601 52.459 61 27 1 18 78 209 267 5.51e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002467 AT5G06800.2 48.905 137 65 1 19 150 191 327 2.84e-34 124
MsaG002467 AT5G06800.1 50.000 132 66 0 19 150 191 322 3.12e-34 124
MsaG002467 AT2G01060.1 46.259 147 69 2 10 150 5 147 3.05e-31 114
MsaG002467 AT4G28610.1 45.775 142 72 2 9 148 217 355 1.06e-30 115
MsaG002467 AT5G29000.1 43.478 138 74 2 17 150 185 322 3.20e-29 110
MsaG002467 AT5G29000.3 43.478 138 74 2 17 150 185 322 3.20e-29 110
MsaG002467 AT5G29000.2 43.478 138 74 2 17 150 228 365 4.72e-29 110
MsaG002467 AT3G04450.4 39.735 151 85 2 4 150 184 332 3.60e-28 108
MsaG002467 AT3G04450.2 39.735 151 85 2 4 150 184 332 3.60e-28 108
MsaG002467 AT3G04450.1 41.007 139 78 1 16 150 234 372 5.78e-28 107
MsaG002467 AT4G13640.1 38.312 154 78 3 20 158 37 188 9.45e-25 97.1
MsaG002467 AT5G18240.1 36.585 164 85 3 2 146 24 187 1.91e-24 97.8
MsaG002467 AT5G18240.4 36.585 164 85 3 2 146 24 187 1.91e-24 97.8
MsaG002467 AT1G79430.2 38.217 157 85 2 9 157 23 175 5.02e-24 96.3
MsaG002467 AT3G13040.3 41.096 146 70 2 16 150 237 377 8.49e-24 96.7
MsaG002467 AT3G13040.1 41.096 146 70 2 16 150 237 377 8.49e-24 96.7
MsaG002467 AT3G13040.2 41.096 146 70 2 16 150 237 377 8.49e-24 96.7
MsaG002467 AT3G04030.3 37.805 164 83 3 2 146 24 187 8.66e-24 96.3
MsaG002467 AT3G24120.1 39.474 152 79 2 20 158 41 192 9.01e-24 94.7
MsaG002467 AT2G20400.2 41.667 132 75 1 20 149 231 362 4.39e-23 94.4
MsaG002467 AT2G20400.1 42.424 132 73 2 20 149 231 361 1.06e-22 93.2
MsaG002467 AT4G13640.2 37.580 157 78 4 20 158 37 191 1.11e-22 91.7
MsaG002467 AT5G18240.5 35.119 168 88 4 2 150 24 189 7.25e-22 90.9
MsaG002467 AT3G24120.2 38.710 155 79 3 20 158 41 195 1.06e-21 89.4
MsaG002467 AT1G69580.1 37.584 149 75 2 15 145 29 177 6.88e-21 87.4
MsaG002467 AT3G04030.2 36.585 164 84 4 2 146 24 186 8.15e-21 87.8
MsaG002467 AT1G69580.2 35.714 154 72 2 15 145 29 178 2.34e-20 86.3
MsaG002467 AT3G12730.1 34.211 152 91 2 5 147 8 159 2.85e-20 84.3
MsaG002467 AT5G18240.2 32.738 168 88 4 2 150 24 185 1.10e-19 84.7
MsaG002467 AT5G18240.3 32.738 168 88 4 2 150 24 185 1.10e-19 84.7
MsaG002467 AT3G04030.1 34.756 164 82 4 2 146 24 181 4.61e-19 83.2
MsaG002467 AT5G45580.1 32.680 153 78 3 20 150 7 156 7.94e-19 80.9
MsaG002467 AT5G45580.2 32.680 153 78 3 20 150 23 172 1.08e-18 80.9
MsaG002467 AT5G29000.4 59.322 59 24 0 17 75 228 286 3.89e-18 79.7
MsaG002467 AT3G04450.3 55.000 60 27 0 16 75 234 293 2.03e-17 77.8
MsaG002467 AT5G06800.3 64.815 54 19 0 20 73 192 245 2.44e-17 77.0
MsaG002467 AT2G42660.1 58.491 53 22 0 22 74 52 104 1.26e-15 72.4
MsaG002467 AT2G40260.1 60.377 53 21 0 22 74 84 136 3.17e-15 72.4
MsaG002467 AT4G04605.1 60.377 53 21 0 22 74 16 68 3.19e-15 70.5
MsaG002467 AT4G04580.1 58.491 53 22 0 22 74 16 68 5.20e-15 68.9
MsaG002467 AT5G42630.2 47.887 71 32 2 22 91 107 173 8.82e-15 69.3
MsaG002467 AT1G14600.1 61.111 54 21 0 22 75 25 78 1.12e-14 69.7
MsaG002467 AT5G16560.1 41.111 90 53 0 22 111 221 310 1.23e-14 70.5
MsaG002467 AT5G42630.1 47.887 71 32 2 22 91 107 173 1.45e-14 69.7
MsaG002467 AT1G32240.2 36.190 105 61 2 22 120 215 319 9.73e-14 67.8
MsaG002467 AT1G32240.1 36.190 105 61 2 22 120 215 319 1.05e-13 67.8
MsaG002467 AT2G38300.1 47.945 73 30 1 22 94 56 120 1.52e-13 67.4
MsaG002467 AT2G01060.2 45.098 102 46 2 55 150 1 98 1.57e-13 66.2
MsaG002467 AT2G02060.2 59.615 52 21 0 22 73 31 82 1.58e-13 66.6
MsaG002467 AT2G02060.1 59.615 52 21 0 22 73 31 82 1.58e-13 66.6
MsaG002467 AT2G06020.1 50.000 54 27 0 20 73 86 139 1.66e-13 67.0
MsaG002467 AT4G37180.1 43.333 90 45 2 5 89 196 284 4.24e-13 66.2
MsaG002467 AT4G17695.1 42.857 91 45 3 22 112 166 249 4.98e-13 65.9
MsaG002467 AT4G37180.2 43.333 90 45 2 5 89 203 291 5.74e-13 65.9
MsaG002467 AT4G04555.1 58.824 51 21 0 24 74 18 68 6.43e-13 62.0
MsaG002467 AT2G25180.1 46.667 75 36 2 17 91 192 262 9.94e-11 59.7

Find 25 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGATTCTTCTATTTGCATT+TGG 0.336254 1:+41826175 None:intergenic
TCATGTCAAAAGTCATTTGC+AGG 0.353917 1:-41827275 MsaT002467.1:intron
ATTATATAAACTTACTTTCT+GGG 0.366001 1:+41827429 None:intergenic
ACAAAAGAAAAGAACCAAAC+AGG 0.442809 1:-41825906 MsaT002467.1:CDS
ATCATTTGTAGTATAGCCTT+AGG 0.450838 1:+41827322 None:intergenic
TAAGGCTATACTACAAATGA+TGG 0.452684 1:-41827320 MsaT002467.1:CDS
TACTTTCTGGGCCACCAAGC+TGG 0.476548 1:+41827441 None:intergenic
ATTATTATTGTCAACCAGCT+TGG 0.483051 1:-41827455 MsaT002467.1:CDS
GATGCAGCGAAATTTGCAAC+TGG 0.519384 1:-41825972 MsaT002467.1:CDS
ATTATTGTCAACCAGCTTGG+TGG 0.523219 1:-41827452 MsaT002467.1:CDS
CACACGCACAAATGTTTGAA+AGG 0.526179 1:-41826984 MsaT002467.1:intron
TTTCTGAAAGGAAATTTCAT+GGG 0.532722 1:+41828474 None:intergenic
AGGGTAAAAGAAGATTAAGA+TGG 0.538823 1:-41827502 MsaT002467.1:CDS
GAGGAAGGGCAGATAACAGA+TGG 0.546455 1:-41826359 MsaT002467.1:CDS
GAGCAATAAACGACAATTCG+AGG 0.546674 1:-41826127 MsaT002467.1:intron
TTAATCAGAGGCAAAGCCTA+AGG 0.566249 1:-41827338 MsaT002467.1:intron
AACTACAACTTGAGGTTGAG+AGG 0.574473 1:-41826149 MsaT002467.1:CDS
GTTTCAGGAATATCTGAGGA+AGG 0.584827 1:-41826374 MsaT002467.1:intron
AATTTCCTTTCAGAAAACTC+AGG 0.594791 1:-41828467 MsaT002467.1:intron
AAGGCTATACTACAAATGAT+GGG 0.610409 1:-41827319 MsaT002467.1:CDS
ATCACGACAACTACAACTTG+AGG 0.638258 1:-41826157 MsaT002467.1:CDS
TTTCAGGAATATCTGAGGAA+GGG 0.639128 1:-41826373 MsaT002467.1:intron
TCGTGTTTCAGGAATATCTG+AGG 0.640339 1:-41826378 MsaT002467.1:intron
TATACTACAAATGATGGGTG+TGG 0.647886 1:-41827314 MsaT002467.1:CDS
AGGAAGGGCAGATAACAGAT+GGG 0.654049 1:-41826358 MsaT002467.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 41825877 41828493 41825877 ID=MsaG002467
Chr1 mRNA 41825877 41828493 41825877 ID=MsaT002467.1;Parent=MsaG002467
Chr1 exon 41825877 41825993 41825877 ID=MsaT002467.1.exon7;Parent=MsaT002467.1
Chr1 CDS 41825877 41825993 41825877 ID=cds.MsaT002467.1;Parent=MsaT002467.1
Chr1 exon 41826128 41826197 41826128 ID=MsaT002467.1.exon6;Parent=MsaT002467.1
Chr1 CDS 41826128 41826197 41826128 ID=cds.MsaT002467.1;Parent=MsaT002467.1
Chr1 exon 41826335 41826389 41826335 ID=MsaT002467.1.exon5;Parent=MsaT002467.1
Chr1 CDS 41826335 41826389 41826335 ID=cds.MsaT002467.1;Parent=MsaT002467.1
Chr1 exon 41826985 41827024 41826985 ID=MsaT002467.1.exon4;Parent=MsaT002467.1
Chr1 CDS 41826985 41827024 41826985 ID=cds.MsaT002467.1;Parent=MsaT002467.1
Chr1 exon 41827276 41827352 41827276 ID=MsaT002467.1.exon3;Parent=MsaT002467.1
Chr1 CDS 41827276 41827352 41827276 ID=cds.MsaT002467.1;Parent=MsaT002467.1
Chr1 exon 41827444 41827550 41827444 ID=MsaT002467.1.exon2;Parent=MsaT002467.1
Chr1 CDS 41827444 41827550 41827444 ID=cds.MsaT002467.1;Parent=MsaT002467.1
Chr1 exon 41828468 41828493 41828468 ID=MsaT002467.1.exon1;Parent=MsaT002467.1
Chr1 CDS 41828468 41828493 41828468 ID=cds.MsaT002467.1;Parent=MsaT002467.1
Gene Sequence

>MsaT002467.1

ATGAAATTTCCTTTCAGAAAACTCAGCAACTCTGATTTTAAGATAAGTGCAAAGGGTAAAAGAAGATTAAGATGGACAAAAGAATTGAATGAGTCATTTATTATTATTGTCAACCAGCTTGGTGGCCCAGAAAAGGCAAAGCCTAAGGCTATACTACAAATGATGGGTGTGGATGTGTTGACCATTTCTCATGTCAAAAGTCATTTGCAGAAATATCGTTCTACATTACACACGCACAAATGTTTGAAAGGAATATCTGAGGAAGGGCAGATAACAGATGGGATTAATGAGCTTCAAGTTAAAATCCAAATGCAAATAGAAGAATCACGACAACTACAACTTGAGGTTGAGAGGAGCAATAAACGACAATTCGAGATGCAGCGAAATTTGCAACTGGTGATTGAACAGCAGAAGAAGCAGCTCAAGTTAATGTTAGATCAACAAAAGAAAAGAACCAAACAGGAGAAAATGACCGATTTAAGACGAAAATGA

Protein sequence

>MsaT002467.1

MKFPFRKLSNSDFKISAKGKRRLRWTKELNESFIIIVNQLGGPEKAKPKAILQMMGVDVLTISHVKSHLQKYRSTLHTHKCLKGISEEGQITDGINELQVKIQMQIEESRQLQLEVERSNKRQFEMQRNLQLVIEQQKKQLKLMLDQQKKRTKQEKMTDLRRK*