AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002469


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002469 MtrunA17_Chr1g0175091 96.273 322 10 2 1 322 97 416 0.0 636
MsaG002469 MtrunA17_Chr7g0258551 60.059 338 105 8 1 325 50 370 7.54e-119 347
MsaG002469 MtrunA17_Chr7g0261911 59.441 143 56 2 154 294 252 394 7.68e-56 188
MsaG002469 MtrunA17_Chr1g0190301 60.563 142 50 3 159 294 166 307 1.44e-51 175
MsaG002469 MtrunA17_Chr2g0291021 50.296 169 66 3 164 314 49 217 6.26e-50 169
MsaG002469 MtrunA17_Chr7g0274471 52.174 161 73 3 163 319 217 377 1.85e-48 168
MsaG002469 MtrunA17_Chr7g0254981 50.323 155 64 3 155 296 33 187 1.17e-47 161
MsaG002469 MtrunA17_Chr6g0461641 45.562 169 66 3 160 306 38 202 1.38e-39 143
MsaG002469 MtrunA17_Chr2g0321461 43.011 186 75 4 142 305 27 203 1.19e-38 140
MsaG002469 MtrunA17_Chr1g0175081 71.111 90 26 0 146 235 4 93 1.21e-38 132
MsaG002469 MtrunA17_Chr1g0175071 42.857 203 108 4 100 300 118 314 5.85e-37 134
MsaG002469 MtrunA17_Chr7g0242521 50.365 137 63 3 152 284 2 137 1.67e-36 131
MsaG002469 MtrunA17_Chr5g0409731 46.154 143 63 2 164 292 25 167 1.13e-35 130
MsaG002469 MtrunA17_Chr4g0042451 42.953 149 70 3 160 294 13 160 6.57e-34 127
MsaG002469 MtrunA17_Chr5g0417521 39.073 151 78 1 160 296 11 161 1.23e-33 126
MsaG002469 MtrunA17_Chr2g0290991 42.038 157 76 4 145 287 21 176 3.39e-31 119
MsaG002469 MtrunA17_Chr8g0356501 44.853 136 70 2 164 294 37 172 5.02e-31 119
MsaG002469 MtrunA17_Chr2g0310011 43.165 139 58 2 160 285 31 161 1.33e-30 118
MsaG002469 MtrunA17_Chr7g0258531 36.522 230 119 6 68 294 139 344 7.35e-30 116
MsaG002469 MtrunA17_Chr1g0197171 40.000 135 70 2 164 287 44 178 2.91e-26 105
MsaG002469 MtrunA17_Chr7g0243081 52.055 73 34 1 166 238 73 144 3.56e-16 78.2
MsaG002469 MtrunA17_Chr8g0379121 58.065 62 25 1 166 227 113 173 4.42e-16 78.6
MsaG002469 MtrunA17_Chr2g0320231 59.677 62 24 1 166 227 99 159 5.62e-16 77.8
MsaG002469 MtrunA17_Chr2g0288601 55.738 61 27 0 154 214 61 121 8.20e-16 73.2
MsaG002469 MtrunA17_Chr6g0479431 60.000 55 22 0 166 220 204 258 1.08e-15 77.0
MsaG002469 MtrunA17_Chr3g0109981 60.000 55 22 0 166 220 276 330 1.88e-15 76.6
MsaG002469 MtrunA17_Chr8g0366281 60.000 55 22 0 166 220 175 229 1.89e-15 76.3
MsaG002469 MtrunA17_Chr5g0410741 60.000 55 22 0 166 220 221 275 1.95e-15 76.6
MsaG002469 MtrunA17_Chr1g0162931 62.069 58 21 1 161 217 14 71 3.32e-15 74.7
MsaG002469 MtrunA17_Chr1g0154261 52.381 63 30 0 166 228 128 190 6.82e-15 73.9
MsaG002469 MtrunA17_Chr1g0210911 63.462 52 19 0 166 217 24 75 7.18e-15 74.3
MsaG002469 MtrunA17_Chr3g0095611 54.237 59 27 0 162 220 17 75 1.38e-14 71.6
MsaG002469 MtrunA17_Chr2g0283951 61.224 49 19 0 166 214 91 139 3.88e-13 69.7
MsaG002469 MtrunA17_Chr7g0275401 54.098 61 27 1 157 217 185 244 7.70e-13 67.8
MsaG002469 MtrunA17_Chr4g0062921 43.820 89 45 1 135 223 211 294 1.02e-12 68.6
MsaG002469 MtrunA17_Chr1g0198601 51.667 60 29 0 164 223 211 270 1.35e-12 67.8
MsaG002469 MtrunA17_Chr4g0009901 50.877 57 27 1 162 218 166 221 3.61e-12 65.9
MsaG002469 MtrunA17_Chr4g0045361 53.448 58 27 0 161 218 271 328 7.85e-12 65.9
MsaG002469 MtrunA17_Chr5g0400241 44.444 81 41 2 143 220 287 366 1.39e-11 65.1
MsaG002469 MtrunA17_Chr5g0402631 54.386 57 26 0 162 218 212 268 1.65e-11 64.3
MsaG002469 MtrunA17_Chr5g0422621 41.304 92 48 2 132 218 162 252 2.03e-11 64.3
MsaG002469 MtrunA17_Chr2g0304841 54.545 55 24 1 164 218 213 266 2.64e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002469 AT5G06800.1 81.679 131 23 1 164 293 192 322 1.22e-70 224
MsaG002469 AT5G06800.2 78.676 136 23 2 164 293 192 327 3.54e-68 218
MsaG002469 AT4G28610.1 56.522 161 63 5 147 304 212 368 2.21e-52 178
MsaG002469 AT5G29000.1 45.833 216 91 6 95 297 124 326 2.42e-52 177
MsaG002469 AT5G29000.3 45.833 216 91 6 95 297 124 326 2.42e-52 177
MsaG002469 AT5G29000.2 45.833 216 91 6 95 297 167 369 5.40e-52 177
MsaG002469 AT2G01060.1 53.086 162 70 4 159 317 10 168 7.94e-51 170
MsaG002469 AT3G04450.4 46.078 204 92 6 116 314 157 347 1.08e-50 173
MsaG002469 AT3G04450.2 46.078 204 92 6 116 314 157 347 1.08e-50 173
MsaG002469 AT3G04450.1 46.078 204 92 6 116 314 197 387 2.29e-50 173
MsaG002469 AT3G13040.3 46.597 191 90 5 137 316 211 400 1.88e-46 163
MsaG002469 AT3G13040.1 46.597 191 90 5 137 316 211 400 1.88e-46 163
MsaG002469 AT3G13040.2 46.597 191 90 5 137 316 211 400 1.88e-46 163
MsaG002469 AT2G20400.1 56.923 130 54 2 164 291 231 360 1.57e-43 154
MsaG002469 AT2G20400.2 56.489 131 54 3 164 291 231 361 8.51e-42 150
MsaG002469 AT5G18240.1 45.181 166 74 3 160 308 41 206 1.82e-39 144
MsaG002469 AT5G18240.4 45.181 166 74 3 160 308 41 206 1.82e-39 144
MsaG002469 AT3G04030.3 49.080 163 61 4 160 305 41 198 2.93e-38 140
MsaG002469 AT5G06800.3 90.769 65 6 0 164 228 192 256 5.19e-37 134
MsaG002469 AT1G69580.1 45.806 155 63 3 160 294 30 183 5.52e-37 135
MsaG002469 AT5G18240.5 44.578 166 73 4 160 308 41 204 2.06e-36 135
MsaG002469 AT1G69580.2 44.516 155 66 2 160 294 30 184 2.51e-36 134
MsaG002469 AT4G13640.1 41.667 168 82 3 143 294 14 181 1.17e-35 131
MsaG002469 AT3G24120.1 42.515 167 75 3 149 294 26 192 1.18e-35 131
MsaG002469 AT3G04030.2 47.853 163 62 5 160 305 41 197 3.59e-35 132
MsaG002469 AT1G79430.2 41.379 174 84 4 127 294 8 169 4.19e-35 131
MsaG002469 AT5G18240.2 42.771 166 72 4 160 308 41 200 5.69e-34 129
MsaG002469 AT5G18240.3 42.771 166 72 4 160 308 41 200 5.69e-34 129
MsaG002469 AT4G13640.2 41.176 170 81 4 143 293 14 183 1.17e-33 125
MsaG002469 AT3G24120.2 41.765 170 75 4 149 294 26 195 1.37e-33 125
MsaG002469 AT5G45580.1 43.590 156 58 4 164 294 7 157 2.21e-33 124
MsaG002469 AT5G45580.2 43.590 156 58 4 164 294 23 173 2.81e-33 124
MsaG002469 AT3G04030.1 46.626 163 59 5 160 305 41 192 7.17e-33 125
MsaG002469 AT2G01060.2 51.639 122 53 4 199 317 1 119 7.54e-31 117
MsaG002469 AT3G12730.1 39.106 179 92 7 164 325 23 201 1.23e-26 105
MsaG002469 AT3G04450.3 65.672 67 23 0 158 224 232 298 1.92e-26 107
MsaG002469 AT5G29000.4 63.768 69 25 0 159 227 226 294 1.36e-25 104
MsaG002469 AT3G04030.5 44.882 127 48 4 196 305 1 122 7.69e-19 85.9
MsaG002469 AT1G79430.1 42.857 105 53 3 196 294 1 104 1.45e-18 84.7
MsaG002469 AT4G04605.1 54.412 68 31 0 153 220 3 70 6.60e-17 79.0
MsaG002469 AT2G42660.1 53.731 67 31 0 162 228 48 114 1.57e-16 78.6
MsaG002469 AT2G06020.1 46.053 76 40 1 145 220 68 142 5.88e-16 77.4
MsaG002469 AT2G38300.1 55.556 63 27 1 166 228 56 117 9.96e-16 77.4
MsaG002469 AT2G02060.2 49.383 81 33 1 145 217 2 82 1.07e-15 76.3
MsaG002469 AT2G02060.1 49.383 81 33 1 145 217 2 82 1.07e-15 76.3
MsaG002469 AT5G16560.1 60.000 55 22 0 166 220 221 275 2.16e-15 76.6
MsaG002469 AT1G32240.2 55.738 61 27 0 166 226 215 275 2.40e-15 76.3
MsaG002469 AT1G32240.1 55.738 61 27 0 166 226 215 275 2.52e-15 76.6
MsaG002469 AT1G14600.1 60.000 55 22 0 166 220 25 79 2.73e-15 75.1
MsaG002469 AT5G18240.6 36.923 130 59 4 196 308 1 124 4.38e-15 75.1
MsaG002469 AT5G42630.2 52.174 69 31 1 166 232 107 175 5.65e-15 73.6
MsaG002469 AT4G17695.1 60.000 55 22 0 166 220 166 220 5.98e-15 74.7
MsaG002469 AT2G40260.1 56.522 69 25 2 157 220 70 138 8.09e-15 75.1
MsaG002469 AT5G42630.1 52.174 69 31 1 166 232 107 175 8.56e-15 73.9
MsaG002469 AT3G04030.4 41.732 127 46 5 196 305 1 116 7.91e-14 71.6
MsaG002469 AT4G04580.1 49.231 65 33 0 153 217 3 67 8.07e-14 68.9
MsaG002469 AT4G37180.1 62.264 53 20 0 166 218 212 264 8.31e-14 71.6
MsaG002469 AT4G37180.2 62.264 53 20 0 166 218 219 271 1.06e-13 71.6
MsaG002469 AT2G03500.1 50.000 70 32 1 164 230 233 302 1.15e-12 68.9
MsaG002469 AT4G04555.1 50.746 67 28 1 156 217 1 67 1.16e-12 63.9
MsaG002469 AT5G58080.1 53.226 62 28 1 157 218 187 247 2.32e-12 68.2
MsaG002469 AT3G16857.3 58.182 55 22 1 164 218 167 220 7.45e-12 66.6
MsaG002469 AT3G16857.1 58.182 55 22 1 164 218 237 290 1.00e-11 66.2
MsaG002469 AT3G16857.2 58.182 55 22 1 164 218 237 290 1.02e-11 66.2
MsaG002469 AT4G37180.3 61.224 49 19 0 166 214 212 260 2.84e-11 63.5
MsaG002469 AT3G25790.1 50.000 58 29 0 169 226 201 258 4.77e-11 63.5
MsaG002469 AT1G49190.1 45.070 71 33 2 164 228 215 285 7.70e-11 63.2
MsaG002469 AT1G25550.1 46.667 75 36 1 164 238 209 279 8.67e-11 62.8

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTGAACACTCAAAATT+TGG 0.140920 1:-41839728 MsaT002469.1:CDS
TCAATATCTTTAGGACTAAT+TGG 0.223277 1:+41839682 None:intergenic
TTCTAATAAGGTTAATGATT+TGG 0.230770 1:-41842554 MsaT002469.1:CDS
TTTCCATTAGAATGAATTAA+TGG 0.240080 1:+41842313 None:intergenic
TCCATTACAAGCAGGGTTTC+CGG 0.269736 1:+41842070 None:intergenic
TACTACTTGTCTTCTGTTGT+TGG 0.302160 1:+41839755 None:intergenic
AGTCCATTAATTCATTCTAA+TGG 0.307810 1:-41842316 MsaT002469.1:CDS
AAATTACCTTGAGCTTGTTC+TGG 0.349173 1:+41841530 None:intergenic
GGAAATGTCTCAACGAATTC+CGG 0.350677 1:-41842089 MsaT002469.1:CDS
CAACACCGAAAGCGATATTA+AGG 0.350678 1:-41841628 MsaT002469.1:CDS
ACATTTCCTGTTGACATAGT+TGG 0.376397 1:+41842104 None:intergenic
ATGGTAACCTCAATATCTTT+AGG 0.378705 1:+41839673 None:intergenic
ATCAGCCTTAATATCGCTTT+CGG 0.385906 1:+41841623 None:intergenic
GAAAATTGAGATATCAAAAG+AGG 0.399023 1:+41842577 None:intergenic
ATTGATTTGTTGTTGAATCA+AGG 0.420361 1:+41842500 None:intergenic
CCACAATATGATTACCAAGT+TGG 0.422118 1:-41842616 MsaT002469.1:CDS
GGAATTCCCTTTGTATCAAA+GGG 0.429673 1:-41842533 MsaT002469.1:CDS
CTGTCTCCAACTATGTCAAC+AGG 0.441628 1:-41842110 MsaT002469.1:CDS
ATTTGAAATTACATGGATGA+TGG 0.447971 1:+41839400 None:intergenic
TACTACTTTCATTTCCAACT+TGG 0.455279 1:+41842602 None:intergenic
GAGAAATTCAGCTGTGAACC+TGG 0.457308 1:+41842149 None:intergenic
TGGAATTCCCTTTGTATCAA+AGG 0.460488 1:-41842534 MsaT002469.1:CDS
TCCGGAAACCCTGCTTGTAA+TGG 0.462303 1:-41842071 MsaT002469.1:CDS
TGAAGGAATTTGAAATTACA+TGG 0.479915 1:+41839393 None:intergenic
AAAATTACAGTTGCGAATAG+AGG 0.499177 1:-41839809 MsaT002469.1:CDS
CCAACTTGGTAATCATATTG+TGG 0.502891 1:+41842616 None:intergenic
AATTAGTCCTAAAGATATTG+AGG 0.504863 1:-41839680 MsaT002469.1:intron
CCCTGCTTGTAATGGATCAT+CGG 0.507035 1:-41842063 MsaT002469.1:CDS
GTTGAGTGTGTGAATCGCCT+TGG 0.507842 1:-41841990 MsaT002469.1:CDS
CTCTAGGAAAATTCCCACAT+TGG 0.519236 1:+41842401 None:intergenic
ACCGATGATCCATTACAAGC+AGG 0.527035 1:+41842062 None:intergenic
CAAGTAAAACACGAATAAGA+TGG 0.534054 1:-41842037 MsaT002469.1:CDS
CAGTGGCTTGCAAATTAGAG+AGG 0.550038 1:-41840646 MsaT002469.1:CDS
CAACAGAAGACAAGTAGTAA+TGG 0.550074 1:-41839751 MsaT002469.1:CDS
CCGATGATCCATTACAAGCA+GGG 0.564544 1:+41842063 None:intergenic
AATTTGACAACTCAAAATTG+TGG 0.568956 1:+41842473 None:intergenic
AAAATTGAGATATCAAAAGA+GGG 0.579511 1:+41842578 None:intergenic
AGCTGCAGCTAGACGTTCAG+AGG 0.587380 1:-41840617 MsaT002469.1:CDS
GAAAGCGATATTAAGGCTGA+TGG 0.589838 1:-41841621 MsaT002469.1:CDS
GAGTGTGTGAATCGCCTTGG+CGG 0.593858 1:-41841987 MsaT002469.1:CDS
TTAAGGCTGATGGATTCAGA+TGG 0.601499 1:-41841611 MsaT002469.1:CDS
AATCCAATAAAATCCAATGT+GGG 0.615499 1:-41842414 MsaT002469.1:CDS
AAATCCAATAAAATCCAATG+TGG 0.616777 1:-41842415 MsaT002469.1:CDS
ACTTACTCTCGGCACCGCCA+AGG 0.616888 1:+41841973 None:intergenic
GCGTCTTCATGAACAACTAG+AGG 0.636358 1:-41840595 MsaT002469.1:intron
TATATGCCAGAACAAGCTCA+AGG 0.643937 1:-41841536 MsaT002469.1:intron
CAGTTGCGAATAGAGGAACA+AGG 0.704497 1:-41839802 MsaT002469.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 41839408 41842647 41839408 ID=MsaG002469
Chr1 mRNA 41839408 41842647 41839408 ID=MsaT002469.1;Parent=MsaG002469
Chr1 exon 41839408 41839425 41839408 ID=MsaT002469.1.exon7;Parent=MsaT002469.1
Chr1 CDS 41839408 41839425 41839408 ID=cds.MsaT002469.1;Parent=MsaT002469.1
Chr1 exon 41839681 41839839 41839681 ID=MsaT002469.1.exon6;Parent=MsaT002469.1
Chr1 CDS 41839681 41839839 41839681 ID=cds.MsaT002469.1;Parent=MsaT002469.1
Chr1 exon 41840596 41840665 41840596 ID=MsaT002469.1.exon5;Parent=MsaT002469.1
Chr1 CDS 41840596 41840665 41840596 ID=cds.MsaT002469.1;Parent=MsaT002469.1
Chr1 exon 41840776 41840827 41840776 ID=MsaT002469.1.exon4;Parent=MsaT002469.1
Chr1 CDS 41840776 41840827 41840776 ID=cds.MsaT002469.1;Parent=MsaT002469.1
Chr1 exon 41841537 41841653 41841537 ID=MsaT002469.1.exon3;Parent=MsaT002469.1
Chr1 CDS 41841537 41841653 41841537 ID=cds.MsaT002469.1;Parent=MsaT002469.1
Chr1 exon 41841979 41842195 41841979 ID=MsaT002469.1.exon2;Parent=MsaT002469.1
Chr1 CDS 41841979 41842195 41841979 ID=cds.MsaT002469.1;Parent=MsaT002469.1
Chr1 exon 41842300 41842647 41842300 ID=MsaT002469.1.exon1;Parent=MsaT002469.1
Chr1 CDS 41842300 41842647 41842300 ID=cds.MsaT002469.1;Parent=MsaT002469.1
Gene Sequence

>MsaT002469.1

ATGGGTTTTCCACAATATGATTACCAAGTTGGAAATGAAAGTAGTAACCCTCTTTTGATATCTCAATTTTCTTCTAATAAGGTTAATGATTTGGAATTCCCTTTGTATCAAAGGGAAAATCATTACCTTGATTCAACAACAAATCAATCTAGCCACAATTTTGAGTTGTCAAATTTAAACACTTTGCAACCAATTCTTAGATCTCCTGAAAAATCCAATAAAATCCAATGTGGGAATTTTCCTAGAGAAAATTTTCTCTCAGTTGAGCAACATAAATTCTTCATTGATGATGCTGCATCAGTTAGTATGAGTCCATTAATTCATTCTAATGGAAATCAAGATCATAAAGTTTCTTGTGGTTCATATGATTTTCCAGGTTCACAGCTGAATTTCTCTTATCAGCAAGATAAACTGTCTCCAACTATGTCAACAGGAAATGTCTCAACGAATTCCGGAAACCCTGCTTGTAATGGATCATCGGTTTCAAGTAAAACACGAATAAGATGGACTCAAGATCTTCATGAGAAGTTTGTTGAGTGTGTGAATCGCCTTGGCGGTGCCGAGAAGGCAACACCGAAAGCGATATTAAGGCTGATGGATTCAGATGGATTGACAATTTTCCATGTTAAAAGTCATTTGCAGAAGTATAGAATTGCAAAATATATGCCAGAACAAGCTCAAGGGAAACCCGAGAAAAGAACTCATGTAGAGAATGTGAATCTTGATGCCAAATCTGGCTTGCAAATTAGAGAGGCACTTCAGCTGCAGCTAGACGTTCAGAGGCGTCTTCATGAACAACTAGAGATTCAGCGAAAATTACAGTTGCGAATAGAGGAACAAGGAAAGCAGTTGAAGATGATGTTTGACCAACAACAGAAGACAAGTAGTAATGGCCAATTGAACACTCAAAATTTGGACAATACAACAAACAATGATATACCAATTAGTCCTAAAGATATTGAGGTTCCATCATCCATGTAA

Protein sequence

>MsaT002469.1

MGFPQYDYQVGNESSNPLLISQFSSNKVNDLEFPLYQRENHYLDSTTNQSSHNFELSNLNTLQPILRSPEKSNKIQCGNFPRENFLSVEQHKFFIDDAASVSMSPLIHSNGNQDHKVSCGSYDFPGSQLNFSYQQDKLSPTMSTGNVSTNSGNPACNGSSVSSKTRIRWTQDLHEKFVECVNRLGGAEKATPKAILRLMDSDGLTIFHVKSHLQKYRIAKYMPEQAQGKPEKRTHVENVNLDAKSGLQIREALQLQLDVQRRLHEQLEIQRKLQLRIEEQGKQLKMMFDQQQKTSSNGQLNTQNLDNTTNNDIPISPKDIEVPSSM*