Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002549 | XP_039691223.1 | 96.809 | 94 | 3 | 0 | 1 | 94 | 193 | 286 | 4.62e-58 | 190 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002549 | sp|Q8VZ42|SAP11_ARATH | 54.255 | 94 | 38 | 1 | 1 | 94 | 190 | 278 | 1.13e-29 | 109 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002549 | tr|B7FKF1|B7FKF1_MEDTR | 96.809 | 94 | 3 | 0 | 1 | 94 | 193 | 286 | 2.21e-58 | 190 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG002549 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002549 | MtrunA17_Chr1g0173421 | 96.809 | 94 | 3 | 0 | 1 | 94 | 193 | 286 | 4.25e-62 | 190 |
| MsaG002549 | MtrunA17_Chr7g0257681 | 63.380 | 71 | 24 | 1 | 25 | 93 | 198 | 268 | 1.41e-25 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002549 | AT2G41835.1 | 54.255 | 94 | 38 | 1 | 1 | 94 | 190 | 278 | 1.15e-30 | 109 |
| MsaG002549 | AT3G57480.2 | 50.000 | 46 | 23 | 0 | 49 | 94 | 203 | 248 | 1.76e-12 | 61.2 |
| MsaG002549 | AT3G57480.1 | 50.000 | 46 | 23 | 0 | 49 | 94 | 203 | 248 | 1.76e-12 | 61.2 |
Find 26 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAGTGATTGAGGAAAACT+TGG | 0.473429 | 1:+42992464 | None:intergenic |
| CACAGATCCCGTGTCCCTTG+TGG | 0.520415 | 1:-42992346 | MsaT002549.1:CDS |
| GCGTGTCCTAAGTGTAGTAA+AGG | 0.526636 | 1:-42992372 | MsaT002549.1:CDS |
| CCCTCTCAACATGCTCCACA+AGG | 0.529390 | 1:+42992331 | None:intergenic |
| AAAACTTGGCACCACATTGA+GGG | 0.531828 | 1:+42992478 | None:intergenic |
| GTGAATCCTTTACTACACTT+AGG | 0.538962 | 1:+42992366 | None:intergenic |
| ACACTTAGGACACGCATCAA+TGG | 0.539521 | 1:+42992380 | None:intergenic |
| ATCGATTAAAGTAGTGATTG+AGG | 0.539536 | 1:+42992455 | None:intergenic |
| GTGGTGAAATTAACCAAGCA+TGG | 0.560055 | 1:-42992557 | MsaT002549.1:CDS |
| TGGTGAAATTAACCAAGCAT+GGG | 0.560973 | 1:-42992556 | MsaT002549.1:CDS |
| GAAAACTTGGCACCACATTG+AGG | 0.568886 | 1:+42992477 | None:intergenic |
| AACATGCTCCACAAGGGACA+CGG | 0.573079 | 1:+42992338 | None:intergenic |
| TGGTGGCACTTCTAGAACAT+AGG | 0.575559 | 1:-42992304 | None:intergenic |
| GAGCAACGACAGTGGTCAAG+TGG | 0.579833 | 1:-42992508 | MsaT002549.1:CDS |
| ACATGCTCCACAAGGGACAC+GGG | 0.604172 | 1:+42992339 | None:intergenic |
| TGGTAACATTCGCAACAGAT+CGG | 0.605303 | 1:+42992400 | None:intergenic |
| GAGCATGTTGAGAGGGAACA+TGG | 0.625868 | 1:-42992324 | MsaT002549.1:CDS |
| CCCTTGTGGAGCATGTTGAG+AGG | 0.626858 | 1:-42992332 | MsaT002549.1:CDS |
| CCTTGTGGAGCATGTTGAGA+GGG | 0.637426 | 1:-42992331 | MsaT002549.1:CDS |
| GGGAGAAAGAGCAACGACAG+TGG | 0.676386 | 1:-42992516 | MsaT002549.1:CDS |
| GGTGAAATTAACCAAGCATG+GGG | 0.687152 | 1:-42992555 | MsaT002549.1:CDS |
| CCTCTCAACATGCTCCACAA+GGG | 0.702395 | 1:+42992332 | None:intergenic |
| GCTGGAATGTCAAAGTTGAG+TGG | 0.723894 | 1:-42992576 | None:intergenic |
| GTGAAATTAACCAAGCATGG+GGG | 0.745364 | 1:-42992554 | MsaT002549.1:CDS |
| CATGTTGAGAGGGAACATGG+TGG | 0.749762 | 1:-42992321 | MsaT002549.1:CDS |
| GTGGAGCAATGCCCTCAATG+TGG | 0.769864 | 1:-42992489 | MsaT002549.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 42992305 | 42992592 | 42992305 | ID=MsaG002549 |
| Chr1 | mRNA | 42992305 | 42992592 | 42992305 | ID=MsaT002549.1;Parent=MsaG002549 |
| Chr1 | exon | 42992305 | 42992592 | 42992305 | ID=MsaT002549.1.exon1;Parent=MsaT002549.1 |
| Chr1 | CDS | 42992305 | 42992592 | 42992305 | ID=cds.MsaT002549.1;Parent=MsaT002549.1 |
| Gene Sequence |
| Protein sequence |
