AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002588

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002588 MtrunA17_Chr1g0203051 56.893 457 172 6 1 439 1 450 3.27e-174 504
MsaG002588 MtrunA17_Chr2g0287651 35.797 433 247 10 17 422 3 431 5.83e-72 244
MsaG002588 MtrunA17_Chr8g0389811 47.101 138 71 1 60 195 58 195 8.97e-43 150
MsaG002588 MtrunA17_Chr5g0448761 29.282 362 221 13 92 421 66 424 7.89e-33 132
MsaG002588 MtrunA17_Chr8g0340801 50.467 107 53 0 195 301 1 107 1.21e-32 120
MsaG002588 MtrunA17_Chr1g0198761 29.808 312 189 10 98 381 59 368 1.28e-30 125
MsaG002588 MtrunA17_Chr1g0202341 24.937 397 250 12 98 459 63 446 2.37e-30 125
MsaG002588 MtrunA17_Chr8g0363841 25.255 392 261 11 98 462 29 415 3.76e-29 121
MsaG002588 MtrunA17_Chr2g0331371 40.625 128 74 1 67 192 36 163 1.76e-27 109
MsaG002588 MtrunA17_Chr1g0171741 34.518 197 120 4 22 214 71 262 2.69e-27 110
MsaG002588 MtrunA17_Chr2g0324011 25.479 365 244 9 98 437 22 383 4.41e-27 114
MsaG002588 MtrunA17_Chr5g0431001 28.076 317 200 8 92 381 67 382 4.59e-27 114
MsaG002588 MtrunA17_Chr2g0290391 26.776 366 228 10 98 425 63 426 6.30e-27 114
MsaG002588 MtrunA17_Chr2g0291591 27.577 388 238 12 98 455 76 450 3.53e-26 112
MsaG002588 MtrunA17_Chr2g0287061 24.694 409 219 15 77 444 165 525 8.20e-24 105
MsaG002588 MtrunA17_Chr7g0258861 26.022 269 180 4 98 348 22 289 2.26e-23 100
MsaG002588 MtrunA17_Chr2g0276841 24.297 391 258 14 97 454 81 466 3.18e-23 103
MsaG002588 MtrunA17_Chr2g0294841 24.450 409 271 13 92 464 51 457 3.90e-23 103
MsaG002588 MtrunA17_Chr6g0473421 42.105 95 53 1 67 159 36 130 7.27e-23 94.7
MsaG002588 MtrunA17_Chr5g0441581 25.000 300 191 7 101 381 47 331 2.96e-22 100
MsaG002588 MtrunA17_Chr2g0296781 25.828 302 195 7 100 381 11 303 1.12e-20 95.5
MsaG002588 MtrunA17_Chr3g0080351 23.844 411 239 14 92 459 82 461 1.34e-20 95.5
MsaG002588 MtrunA17_Chr1g0207551 23.913 368 238 13 92 432 40 392 1.18e-19 92.4
MsaG002588 MtrunA17_Chr3g0082121 24.804 383 233 12 77 421 28 393 2.26e-19 91.7
MsaG002588 MtrunA17_Chr1g0172931 41.818 110 62 1 59 166 49 158 3.30e-19 84.7
MsaG002588 MtrunA17_Chr2g0329031 24.422 389 235 14 92 444 20 385 4.34e-19 90.5
MsaG002588 MtrunA17_Chr6g0477261 28.796 191 120 3 207 381 2 192 3.46e-17 84.7
MsaG002588 MtrunA17_Chr6g0458391 26.432 227 138 7 60 260 66 289 2.58e-15 79.0
MsaG002588 MtrunA17_Chr5g0440451 23.418 316 192 10 98 378 55 355 6.47e-15 77.8
MsaG002588 MtrunA17_Chr1g0203721 23.102 303 213 6 98 381 75 376 5.78e-14 74.7
MsaG002588 MtrunA17_Chr2g0291581 22.513 382 233 12 98 444 83 436 8.72e-14 73.9
MsaG002588 MtrunA17_Chr7g0228051 51.471 68 30 1 31 95 18 85 4.18e-12 62.4
MsaG002588 MtrunA17_Chr5g0448511 27.027 185 110 5 261 422 15 197 6.17e-12 67.8
MsaG002588 MtrunA17_Chr3g0118611 25.106 235 150 5 249 460 110 341 6.42e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002588 AT4G38180.1 30.141 355 215 11 92 417 79 429 3.97e-34 137
MsaG002588 AT5G28530.3 27.793 367 223 11 98 425 67 430 6.06e-34 135
MsaG002588 AT5G28530.2 27.793 367 223 11 98 425 67 430 6.06e-34 135
MsaG002588 AT5G28530.1 27.793 367 223 11 98 425 67 430 6.11e-34 135
MsaG002588 AT1G10240.2 26.010 396 245 15 97 454 59 444 2.63e-25 110
MsaG002588 AT1G10240.1 26.010 396 245 15 97 454 59 444 2.63e-25 110
MsaG002588 AT5G28530.4 25.513 341 201 11 98 425 67 367 1.77e-21 98.2
MsaG002588 AT5G28530.5 25.513 341 201 11 98 425 67 367 1.77e-21 98.2
MsaG002588 AT4G15090.1 26.384 307 202 10 92 378 57 359 9.41e-21 96.3
MsaG002588 AT4G15090.5 26.384 307 202 10 92 378 57 359 9.41e-21 96.3
MsaG002588 AT4G15090.3 26.384 307 202 10 92 378 57 359 9.41e-21 96.3
MsaG002588 AT4G15090.2 26.384 307 202 10 92 378 57 359 9.41e-21 96.3
MsaG002588 AT4G15090.4 26.384 307 202 10 92 378 57 359 9.41e-21 96.3
MsaG002588 AT1G80010.1 26.005 373 210 16 98 425 107 458 9.81e-21 96.3
MsaG002588 AT3G06250.2 23.833 407 235 12 98 476 202 561 1.57e-20 95.5
MsaG002588 AT3G06250.1 23.833 407 235 12 98 476 202 561 1.57e-20 95.5
MsaG002588 AT3G06250.3 23.833 407 235 12 98 476 202 561 1.57e-20 95.5
MsaG002588 AT1G52520.1 23.670 376 238 12 98 444 97 452 1.56e-19 92.4
MsaG002588 AT5G18960.2 24.282 383 222 12 98 454 223 563 1.85e-19 92.4
MsaG002588 AT5G18960.1 24.282 383 222 12 98 454 223 563 1.85e-19 92.4
MsaG002588 AT1G76320.3 25.266 376 237 13 98 444 9 369 2.12e-18 89.0
MsaG002588 AT1G76320.2 25.131 382 242 13 92 444 3 369 3.09e-18 88.6
MsaG002588 AT1G76320.1 25.131 382 242 13 92 444 3 369 3.90e-18 88.2
MsaG002588 AT1G76320.4 25.131 382 242 13 92 444 3 369 3.90e-18 88.2
MsaG002588 AT3G22170.2 24.224 322 184 10 92 380 76 370 3.48e-16 82.0
MsaG002588 AT3G22170.1 24.224 322 184 10 92 380 76 370 3.48e-16 82.0
MsaG002588 AT2G27110.2 21.705 387 254 12 81 444 43 403 5.97e-14 75.1
MsaG002588 AT2G27110.1 21.705 387 254 12 81 444 43 403 5.97e-14 75.1

Find 105 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAACTTGGGACAAATTT+TGG 0.083842 1:-43522729 None:intergenic
TCATGAAATTCTAGAAATTT+TGG 0.094124 1:+43521401 MsaT002588.1:CDS
GAACACTTCGACATTTATTT+TGG 0.173268 1:+43522367 MsaT002588.1:CDS
GATAAAATCTACCACGGATA+TGG 0.184158 1:-43522881 None:intergenic
GAAGGTCTTCACTCTGAATT+TGG 0.235885 1:+43522930 MsaT002588.1:CDS
TGTTACCGTCTTGTCGAATT+TGG 0.240334 1:+43522959 MsaT002588.1:CDS
CTCGGTGTTTGGAGATGTTT+TGG 0.257555 1:+43522416 MsaT002588.1:CDS
GGATTTGTTGGTGCAGAGTT+TGG 0.279208 1:+43522798 MsaT002588.1:CDS
TCCCAATGTGTTTACATAAT+CGG 0.282947 1:+43521427 MsaT002588.1:CDS
CAACTAAACGATCCTGAAAA+TGG 0.285819 1:-43521780 None:intergenic
CCCAATGTGTTTACATAATC+GGG 0.288280 1:+43521428 MsaT002588.1:CDS
CTCATACTTCCCATCCAAAA+AGG 0.333792 1:-43522067 None:intergenic
GATGCAATTGATTTCCTTAA+GGG 0.353772 1:+43522291 MsaT002588.1:CDS
GAACATACACCGTTTGAACT+TGG 0.355777 1:-43522741 None:intergenic
ATTCCTACGTCACGCATCTA+TGG 0.380981 1:+43522162 MsaT002588.1:CDS
GGATGCAATTGATTTCCTTA+AGG 0.395881 1:+43522290 MsaT002588.1:CDS
TTTGTCATAGAGAAGGTTAC+AGG 0.397074 1:+43521905 MsaT002588.1:CDS
GATTTGTTGGTGCAGAGTTT+GGG 0.404198 1:+43522799 MsaT002588.1:CDS
GTACGGATGATATGATGTTT+TGG 0.413634 1:+43522322 MsaT002588.1:CDS
TCACCGCTTGTGCCCGCACT+TGG 0.442341 1:+43522678 MsaT002588.1:CDS
CGTATGGACTACTCGGTGTT+TGG 0.442365 1:+43522405 MsaT002588.1:CDS
AACATACACCGTTTGAACTT+GGG 0.446441 1:-43522740 None:intergenic
ACAACGAGCAATTCAAGAAC+AGG 0.446586 1:+43522244 MsaT002588.1:CDS
AAAGCCGTGTTGTTAAGAAC+AGG 0.447436 1:+43521854 MsaT002588.1:CDS
CAACAATTTCTTGAGGCAAT+GGG 0.447655 1:+43522576 MsaT002588.1:CDS
TGAAACTATGTGGGATTTGT+TGG 0.448927 1:+43522786 MsaT002588.1:CDS
CCCGATTATGTAAACACATT+GGG 0.450180 1:-43521428 None:intergenic
CCTTAAGGGTTTGTGTAGTA+CGG 0.450651 1:+43522305 MsaT002588.1:CDS
CCGTACTACACAAACCCTTA+AGG 0.451554 1:-43522305 None:intergenic
GACATGTACAGTTCGTTGTA+TGG 0.455744 1:+43521664 MsaT002588.1:CDS
ATAAAATCTACCACGGATAT+GGG 0.462618 1:-43522880 None:intergenic
TACCGTCTTGTCGAATTTGG+TGG 0.463178 1:+43522962 MsaT002588.1:CDS
TTGGGTTGGAAAATAATAAG+TGG 0.477062 1:+43522817 MsaT002588.1:CDS
GCAACAATTTCTTGAGGCAA+TGG 0.488678 1:+43522575 MsaT002588.1:CDS
GAGAGCAGAAGGCAACTAGT+AGG 0.491224 1:+43521956 MsaT002588.1:CDS
TTGAAGAGTTTGAAACTATG+TGG 0.492334 1:+43522776 MsaT002588.1:CDS
AACTAGTAGGTGTGGTTGCA+TGG 0.492607 1:+43521969 MsaT002588.1:CDS
ATGAAAATGATGAGGAAAGC+TGG 0.493711 1:+43522138 MsaT002588.1:CDS
CATGGACAATAGCTCTTGTT+CGG 0.498690 1:+43521606 MsaT002588.1:CDS
TTGGAGGCACACAGTTAAAG+AGG 0.502132 1:+43522341 MsaT002588.1:CDS
TGCGGAAGGTTCAGAGGTAG+AGG 0.504723 1:+43521464 MsaT002588.1:CDS
CATAGAGAAGGTTACAGGGT+TGG 0.507010 1:+43521910 MsaT002588.1:CDS
TGAAGAGTTTGAAACTATGT+GGG 0.510185 1:+43522777 MsaT002588.1:CDS
AAATTTGTCCCAAGTTCAAA+CGG 0.514539 1:+43522732 MsaT002588.1:CDS
TCGCTCATCTTCCTATGTGC+AGG 0.519995 1:-43522099 None:intergenic
GCGACCTGCGTAAGTGTCCT+CGG 0.521720 1:-43522551 None:intergenic
GTGTGATGGGGTTAGCCGTA+TGG 0.524800 1:+43522389 MsaT002588.1:CDS
CCTTAAAATTAATTCTGTCA+AGG 0.525060 1:-43521729 None:intergenic
TTGCATTTCTAGCCAAGTGC+GGG 0.532211 1:-43522690 None:intergenic
TAGCCAAGTGCGGGCACAAG+CGG 0.536580 1:-43522681 None:intergenic
TGTTGGTGCAGAGTTTGGGT+TGG 0.541975 1:+43522803 MsaT002588.1:CDS
ACCTTTGTTTGTCATAGAGA+AGG 0.542041 1:+43521898 MsaT002588.1:CDS
ATATTTGGTAGTGCGATTGT+TGG 0.542911 1:+43522520 MsaT002588.1:intron
GTGCAACTTTGACGATGAAA+CGG 0.547511 1:+43521687 MsaT002588.1:CDS
CCTCTGAACCTTCCGCACTA+AGG 0.548938 1:-43521458 None:intergenic
TTCTTGAGGCAATGGGCGGA+AGG 0.549926 1:+43522583 MsaT002588.1:CDS
ACATGTACAGTTCGTTGTAT+GGG 0.550841 1:+43521665 MsaT002588.1:CDS
CGATAGGAAAAGAGAGCAGA+AGG 0.554726 1:+43521945 MsaT002588.1:CDS
CCCCGATTATGTAAACACAT+TGG 0.555220 1:-43521429 None:intergenic
AATTTGTACGGTTGTGTACG+TGG 0.562187 1:+43521817 MsaT002588.1:CDS
GAGGAAGAGTACAGATGCAG+AGG 0.570853 1:+43522269 MsaT002588.1:CDS
GTTGCATTTCTAGCCAAGTG+CGG 0.576735 1:-43522691 None:intergenic
GGAGAGTGATGTATGTTCCT+CGG 0.577170 1:+43521627 MsaT002588.1:CDS
TGAAACGGCGGTTGATTGCA+TGG 0.577332 1:+43521702 MsaT002588.1:CDS
AGCAATTCAAGAACAGGGAG+AGG 0.577733 1:+43522250 MsaT002588.1:CDS
CAGTATGATAAGCGGAGCAA+TGG 0.586661 1:+43522850 MsaT002588.1:CDS
CAGAAGGCAACTAGTAGGTG+TGG 0.587238 1:+43521961 MsaT002588.1:CDS
GTACACGTTGACGCTGGTAG+CGG 0.591900 1:+43522003 MsaT002588.1:CDS
TTGACGCTGGTAGCGGTCGA+TGG 0.592302 1:+43522010 MsaT002588.1:CDS
CCTTAGTGCGGAAGGTTCAG+AGG 0.595778 1:+43521458 MsaT002588.1:CDS
GTGAAGACCTTCACACCTTG+AGG 0.596000 1:-43522919 None:intergenic
GGATGCAACCTTAGTGCGGA+AGG 0.596973 1:+43521450 MsaT002588.1:CDS
AGGCACACAGTTAAAGAGGA+TGG 0.598221 1:+43522345 MsaT002588.1:CDS
CGTGTTGTTAAGAACAGGGA+TGG 0.599865 1:+43521859 MsaT002588.1:CDS
TTGTCATAGAGAAGGTTACA+GGG 0.601328 1:+43521906 MsaT002588.1:CDS
TTTGGTTTCGAACAACCTCA+AGG 0.606051 1:+43522904 MsaT002588.1:CDS
CATCCCTGTTCTTAACAACA+CGG 0.608258 1:-43521858 None:intergenic
ATCCACCAAATTCGACAAGA+CGG 0.610939 1:-43522964 None:intergenic
ACCTTCTCTATGACAAACAA+AGG 0.615884 1:-43521899 None:intergenic
CAATTTCTTGAGGCAATGGG+CGG 0.622996 1:+43522579 MsaT002588.1:CDS
CACAAGCGGTGATGAGCATG+TGG 0.624762 1:-43522667 None:intergenic
AGGGAATGCTACCTGCACAT+AGG 0.625271 1:+43522088 MsaT002588.1:CDS
TTGGAAGTAATGCTGCCGAT+AGG 0.626637 1:+43521929 MsaT002588.1:CDS
GGTTAGCCGTATGGACTACT+CGG 0.633294 1:+43522398 MsaT002588.1:CDS
AATACCAAATGAAAATGATG+AGG 0.636392 1:+43522130 MsaT002588.1:CDS
CGAACAACCTCAAGGTGTGA+AGG 0.638864 1:+43522912 MsaT002588.1:CDS
CCAATGTGTTTACATAATCG+GGG 0.641860 1:+43521429 MsaT002588.1:CDS
AAACCATAGATGCGTGACGT+AGG 0.642746 1:-43522165 None:intergenic
CAAACACCGAGTAGTCCATA+CGG 0.644760 1:-43522404 None:intergenic
CAACGAGCAATTCAAGAACA+GGG 0.647223 1:+43522245 MsaT002588.1:CDS
GTCTTCGACATCTTCATCCG+AGG 0.652680 1:-43521644 None:intergenic
TGGTCAACCGCCCATATCCG+TGG 0.653866 1:+43522870 MsaT002588.1:CDS
ACCAAAGATAAAATCTACCA+CGG 0.656197 1:-43522887 None:intergenic
GTCGCCGCAACAATTTCTTG+AGG 0.660431 1:+43522569 MsaT002588.1:CDS
ATTGCCTCAAGAAATTGTTG+CGG 0.662569 1:-43522573 None:intergenic
ATTCAAGTACACGTTGACGC+TGG 0.666323 1:+43521997 MsaT002588.1:CDS
TCGTGAGTCAGTATGATAAG+CGG 0.680593 1:+43522842 MsaT002588.1:CDS
TCGGGGATGCAACCTTAGTG+CGG 0.681157 1:+43521446 MsaT002588.1:CDS
AAGCCGTGTTGTTAAGAACA+GGG 0.681295 1:+43521855 MsaT002588.1:CDS
AAATCTACCACGGATATGGG+CGG 0.686814 1:-43522877 None:intergenic
GAAACCGAGGACACTTACGC+AGG 0.690903 1:+43522547 MsaT002588.1:CDS
ACTGTGTCGATTATAATCGA+CGG 0.692028 1:+43522607 MsaT002588.1:CDS
CAACTTTGACGATGAAACGG+CGG 0.704083 1:+43521690 MsaT002588.1:CDS
TAGGGTCGGATGAAATCATG+CGG 0.716997 1:+43521755 MsaT002588.1:CDS
GATTGTTGGTGATGAAACCG+AGG 0.769466 1:+43522534 MsaT002588.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 43521393 43523014 43521393 ID=MsaG002588
Chr1 mRNA 43521393 43523014 43521393 ID=MsaT002588.1;Parent=MsaG002588
Chr1 exon 43521393 43521485 43521393 ID=MsaT002588.1.exon1;Parent=MsaT002588.1
Chr1 CDS 43521393 43521485 43521393 ID=cds.MsaT002588.1;Parent=MsaT002588.1
Chr1 exon 43521604 43522493 43521604 ID=MsaT002588.1.exon2;Parent=MsaT002588.1
Chr1 CDS 43521604 43522493 43521604 ID=cds.MsaT002588.1;Parent=MsaT002588.1
Chr1 exon 43522531 43523014 43522531 ID=MsaT002588.1.exon3;Parent=MsaT002588.1
Chr1 CDS 43522531 43523014 43522531 ID=cds.MsaT002588.1;Parent=MsaT002588.1
Gene Sequence

>MsaT002588.1

ATGGATATTCATGAAATTCTAGAAATTTTGGAATTCCCAATGTGTTTACATAATCGGGGATGCAACCTTAGTGCGGAAGGTTCAGAGGTAGAGGACATGGACAATAGCTCTTGTTCGGAGAGTGATGTATGTTCCTCGGATGAAGATGTCGAAGACATGTACAGTTCGTTGTATGGGTGCAACTTTGACGATGAAACGGCGGTTGATTGCATGGCTGACCTTGACAGAATTAATTTTAAGGAAATAGGGTCGGATGAAATCATGCGGTACCATTTTCAGGATCGTTTAGTTGCATTCAAGTTTTACAATTTGTACGGTTGTGTACGTGGATTTTCCGGTAGAAAAAGCCGTGTTGTTAAGAACAGGGATGGCGATGTTGTTCAACAAACCTTTGTTTGTCATAGAGAAGGTTACAGGGTTGGAAGTAATGCTGCCGATAGGAAAAGAGAGCAGAAGGCAACTAGTAGGTGTGGTTGCATGGCAAAAATTCAAGTACACGTTGACGCTGGTAGCGGTCGATGGTACATAAAATCTGTTGACGATATTCACAATCACACCTTTTTGGATGGGAAGTATGAGGGAATGCTACCTGCACATAGGAAGATGAGCGATTATGACAAATACCAAATGAAAATGATGAGGAAAGCTGGCATTCCTACGTCACGCATCTATGGTTTTTTTGCTTCACAGGCTGGTGGATTTAAAAATCTTGGATATACAAGAAGAGACATGTACAACGAGCAATTCAAGAACAGGGAGAGGAAGAGTACAGATGCAGAGGATGCAATTGATTTCCTTAAGGGTTTGTGTAGTACGGATGATATGATGTTTTGGAGGCACACAGTTAAAGAGGATGGAACACTTCGACATTTATTTTGGTGTGATGGGGTTAGCCGTATGGACTACTCGGTGTTTGGAGATGTTTTGGCATTTGATGCTACATATCGAAAAATTAAGTACAACACACCGTTAGTTATTTTTCCTGCGATTGTTGGTGATGAAACCGAGGACACTTACGCAGGTCGCCGCAACAATTTCTTGAGGCAATGGGCGGAAGGCACTGTGTCGATTATAATCGACGGAGATCTATCAATGAGAAATGCAATTAGAAGAGTATTTCCACATGCTCATCACCGCTTGTGCCCGCACTTGGCTAGAAATGCAACGAGCAATATAAAAATCCAAAATTTGTCCCAAGTTCAAACGGTGTATGTTCGCGATTTTGATATTGAAGAGTTTGAAACTATGTGGGATTTGTTGGTGCAGAGTTTGGGTTGGAAAATAATAAGTGGATTCGTGAGTCAGTATGATAAGCGGAGCAATGGTCAACCGCCCATATCCGTGGTAGATTTTATCTTTGGTTTCGAACAACCTCAAGGTGTGAAGGTCTTCACTCTGAATTTGGAAAATATGTTACCGTCTTGTCGAATTTGGTGGATTTTTTACAGCATTTTTTGCGGTGGCTGA
Protein sequence

>MsaT002588.1

MDIHEILEILEFPMCLHNRGCNLSAEGSEVEDMDNSSCSESDVCSSDEDVEDMYSSLYGCNFDDETAVDCMADLDRINFKEIGSDEIMRYHFQDRLVAFKFYNLYGCVRGFSGRKSRVVKNRDGDVVQQTFVCHREGYRVGSNAADRKREQKATSRCGCMAKIQVHVDAGSGRWYIKSVDDIHNHTFLDGKYEGMLPAHRKMSDYDKYQMKMMRKAGIPTSRIYGFFASQAGGFKNLGYTRRDMYNEQFKNRERKSTDAEDAIDFLKGLCSTDDMMFWRHTVKEDGTLRHLFWCDGVSRMDYSVFGDVLAFDATYRKIKYNTPLVIFPAIVGDETEDTYAGRRNNFLRQWAEGTVSIIIDGDLSMRNAIRRVFPHAHHRLCPHLARNATSNIKIQNLSQVQTVYVRDFDIEEFETMWDLLVQSLGWKIISGFVSQYDKRSNGQPPISVVDFIFGFEQPQGVKVFTLNLENMLPSCRIWWIFYSIFCGG*