AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002614

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002614 MtrunA17_Chr8g0370121 67.133 143 31 1 1 127 99 241 2.99e-65 197
MsaG002614 MtrunA17_Chr8g0370131 62.791 129 39 5 2 128 103 224 1.20e-45 147
MsaG002614 MtrunA17_Chr5g0436501 39.744 78 42 2 2 79 125 197 2.66e-13 64.7
MsaG002614 MtrunA17_Chr5g0429521 36.538 104 49 5 2 104 132 219 8.60e-13 63.5
MsaG002614 MtrunA17_Chr5g0409841 40.541 74 41 1 2 75 92 162 1.24e-12 62.4
MsaG002614 MtrunA17_Chr3g0104071 51.064 47 23 0 2 48 147 193 1.46e-12 62.8
MsaG002614 MtrunA17_Chr4g0062741 37.179 78 40 2 2 74 138 211 3.40e-12 61.6
MsaG002614 MtrunA17_Chr2g0331031 33.010 103 55 4 2 97 109 204 4.07e-12 61.6
MsaG002614 MtrunA17_Chr7g0241761 48.936 47 24 0 2 48 140 186 1.31e-11 60.1
MsaG002614 MtrunA17_Chr1g0162081 46.000 50 27 0 2 51 122 171 1.66e-11 59.7
MsaG002614 MtrunA17_Chr8g0389241 38.806 67 36 1 2 68 105 166 1.69e-11 59.7
MsaG002614 MtrunA17_Chr1g0210471 39.437 71 41 1 2 72 124 192 4.39e-11 58.2
MsaG002614 MtrunA17_Chr5g0415191 46.809 47 25 0 2 48 102 148 8.69e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002614 AT5G58900.1 53.191 47 22 0 2 48 141 187 5.03e-13 64.3
MsaG002614 AT2G38090.1 42.424 66 34 1 2 67 141 202 1.36e-12 63.2
MsaG002614 AT3G11280.2 47.917 48 25 0 2 49 127 174 1.72e-12 62.8
MsaG002614 AT3G11280.1 47.917 48 25 0 2 49 127 174 1.72e-12 62.8
MsaG002614 AT5G05790.2 47.917 48 25 0 2 49 131 178 3.56e-12 62.0
MsaG002614 AT5G05790.1 47.917 48 25 0 2 49 131 178 3.56e-12 62.0
MsaG002614 AT1G49010.1 46.809 47 25 0 2 48 134 180 5.01e-12 61.6
MsaG002614 AT5G01200.1 49.206 63 27 2 2 63 146 204 6.21e-12 61.2
MsaG002614 AT5G56840.1 34.615 78 51 0 2 79 91 168 2.42e-11 59.3
MsaG002614 AT5G08520.1 46.000 50 27 0 2 51 118 167 3.05e-11 59.3
MsaG002614 AT3G16350.1 46.809 47 25 0 2 48 137 183 6.22e-11 58.9
MsaG002614 AT5G04760.1 39.437 71 41 1 2 72 99 167 6.79e-11 57.8

Find 27 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATAAAATGTTGGAACTTC+TGG 0.186491 1:+43887368 None:intergenic
ACACATATGTGCATGGTTTA+AGG 0.242121 1:-43887454 MsaT002614.1:CDS
AGTTGATGTGGAACTTCGTT+TGG 0.343928 1:-43887258 MsaT002614.1:CDS
CCTGTTGTTTCTGTTGATGA+TGG 0.376916 1:-43887284 MsaT002614.1:CDS
GTGATGGGGATGATTAAGTA+TGG 0.403039 1:-43887575 MsaT002614.1:CDS
TGTCTTAGCTTATTAGTGAT+GGG 0.419172 1:-43887590 MsaT002614.1:intron
GTTGATGTGGAACTTCGTTT+GGG 0.430893 1:-43887257 MsaT002614.1:CDS
TTAAGTATGGAAAAGGGAAA+TGG 0.433708 1:-43887562 MsaT002614.1:CDS
GGGATGATTAAGTATGGAAA+AGG 0.439099 1:-43887569 MsaT002614.1:CDS
GCTATGCTAATAGCTTCTTC+AGG 0.441719 1:-43887487 MsaT002614.1:CDS
ATGTGCATGGTTTAAGGAAA+AGG 0.449777 1:-43887448 MsaT002614.1:CDS
GATCTAGGCGCATGGTTCCT+TGG 0.456488 1:-43888217 None:intergenic
CCATCATCAACAGAAACAAC+AGG 0.464747 1:+43887284 None:intergenic
TTGTCTTAGCTTATTAGTGA+TGG 0.474565 1:-43887591 MsaT002614.1:intron
AGTGCTTGCTTCATAGTTGT+TGG 0.477998 1:+43887319 None:intergenic
AAACCATGCACATATGTGTC+TGG 0.497935 1:+43887458 None:intergenic
GTCTTAGCTTATTAGTGATG+GGG 0.500061 1:-43887589 MsaT002614.1:intron
ATGGTTTAAGGAAAAGGAAA+AGG 0.505645 1:-43887442 MsaT002614.1:CDS
GCATAGCTTTGAACTTGTTG+TGG 0.507199 1:+43887503 None:intergenic
GGATGATTAAGTATGGAAAA+GGG 0.561038 1:-43887568 MsaT002614.1:CDS
GCTTCTCCTCCATAAAATGT+TGG 0.561715 1:+43887359 None:intergenic
TGTTAACAATGATCTTGCAA+AGG 0.578401 1:-43887408 MsaT002614.1:CDS
AGAAACAACAGGAAGAGTCA+TGG 0.595597 1:+43887295 None:intergenic
TCATGCTCTAACTGACTCCA+AGG 0.609370 1:+43888200 None:intergenic
CATGCACATATGTGTCTGGT+AGG 0.610902 1:+43887462 None:intergenic
TGATGATGGAAAAGTTGATG+TGG 0.639976 1:-43887270 MsaT002614.1:CDS
CTACCAGACACATATGTGCA+TGG 0.648995 1:-43887461 MsaT002614.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 43887247 43888228 43887247 ID=MsaG002614
Chr1 mRNA 43887247 43888228 43887247 ID=MsaT002614.1;Parent=MsaG002614
Chr1 exon 43887247 43887604 43887247 ID=MsaT002614.1.exon2;Parent=MsaT002614.1
Chr1 CDS 43887247 43887604 43887247 ID=cds.MsaT002614.1;Parent=MsaT002614.1
Chr1 exon 43888200 43888228 43888200 ID=MsaT002614.1.exon1;Parent=MsaT002614.1
Chr1 CDS 43888200 43888228 43888200 ID=cds.MsaT002614.1;Parent=MsaT002614.1
Gene Sequence

>MsaT002614.1

ATGGTTCCTTGGAGTCAGTTAGAGCATGACTTATTAGTGATGGGGATGATTAAGTATGGAAAAGGGAAATGGAGTGAAATTGCTAAGAACTTTGTGTGCAACAAGACTCCACAACAAGTTCAAAGCTATGCTAATAGCTTCTTCAGGTACCTACCAGACACATATGTGCATGGTTTAAGGAAAAGGAAAAGGAACAACATGATTGTTAACAATGATCTTGCAAAGGAAACTCTCACTCTTTTTCCAGAAGTTCCAACATTTTATGGAGGAGAAGCTTGCAGTAGCAAGAACACCAACAACTATGAAGCAAGCACTTCCATGACTCTTCCTGTTGTTTCTGTTGATGATGGAAAAGTTGATGTGGAACTTCGTTTGGGTTTAAATTAG
Protein sequence

>MsaT002614.1

MVPWSQLEHDLLVMGMIKYGKGKWSEIAKNFVCNKTPQQVQSYANSFFRYLPDTYVHGLRKRKRNNMIVNNDLAKETLTLFPEVPTFYGGEACSSKNTNNYEASTSMTLPVVSVDDGKVDVELRLGLN*