AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002714

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002714 MtrunA17_Chr1g0177091 97.289 332 9 0 1 332 1 332 0.0 676
MsaG002714 MtrunA17_Chr7g0267581 70.517 329 88 6 1 324 1 325 1.96e-162 456
MsaG002714 MtrunA17_Chr8g0352251 50.400 125 49 4 128 241 109 231 5.78e-30 115
MsaG002714 MtrunA17_Chr2g0300261 49.624 133 49 4 116 231 14 145 1.51e-29 113
MsaG002714 MtrunA17_Chr2g0292411 50.467 107 51 1 133 237 80 186 1.44e-28 109
MsaG002714 MtrunA17_Chr3g0103391 50.000 116 44 4 127 231 125 237 4.77e-27 108
MsaG002714 MtrunA17_Chr8g0343451 47.414 116 46 4 120 222 110 223 5.61e-26 105
MsaG002714 MtrunA17_Chr2g0329811 36.723 177 100 4 95 260 108 283 5.17e-25 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002714 AT5G06510.1 44.518 301 109 13 29 325 13 259 1.67e-61 197
MsaG002714 AT5G06510.5 44.518 301 109 13 29 325 13 259 1.67e-61 197
MsaG002714 AT5G06510.2 44.518 301 109 13 29 325 13 259 1.67e-61 197
MsaG002714 AT5G06510.6 44.518 301 109 13 29 325 13 259 1.67e-61 197
MsaG002714 AT3G05690.1 44.237 321 110 17 27 326 12 284 1.22e-56 186
MsaG002714 AT3G05690.2 44.237 321 110 17 27 326 49 321 8.47e-56 185
MsaG002714 AT5G06510.3 48.511 235 76 9 93 325 19 210 1.01e-55 181
MsaG002714 AT5G06510.4 51.269 197 58 8 131 325 4 164 6.72e-49 162
MsaG002714 AT5G06510.7 51.269 197 58 8 131 325 4 164 6.72e-49 162
MsaG002714 AT1G30500.1 50.420 119 53 1 127 239 53 171 3.35e-32 119
MsaG002714 AT2G34720.1 40.462 173 86 5 97 256 19 187 3.44e-32 119
MsaG002714 AT1G30500.2 49.593 123 52 1 127 239 53 175 1.81e-31 117
MsaG002714 AT2G34720.2 46.377 138 58 4 132 256 18 152 2.64e-30 113
MsaG002714 AT2G34720.3 46.377 138 58 4 132 256 18 152 2.64e-30 113
MsaG002714 AT1G72830.1 42.515 167 74 6 132 284 135 293 1.03e-29 116
MsaG002714 AT5G12840.4 34.266 286 150 12 1 266 1 268 4.70e-29 113
MsaG002714 AT5G12840.2 34.266 286 150 12 1 266 1 268 4.70e-29 113
MsaG002714 AT5G12840.5 45.033 151 65 5 127 266 126 269 2.30e-28 111
MsaG002714 AT5G12840.3 45.033 151 65 5 127 266 126 269 2.30e-28 111
MsaG002714 AT5G12840.1 45.033 151 65 5 127 266 126 269 2.30e-28 111
MsaG002714 AT1G72830.2 42.262 168 74 7 132 284 135 294 4.22e-28 112
MsaG002714 AT1G17590.3 59.259 81 31 2 143 223 153 231 6.14e-26 105
MsaG002714 AT1G17590.2 59.259 81 31 2 143 223 153 231 6.14e-26 105
MsaG002714 AT1G17590.4 59.259 81 31 2 143 223 153 231 6.14e-26 105
MsaG002714 AT1G17590.1 59.259 81 31 2 143 223 153 231 6.14e-26 105
MsaG002714 AT1G54160.1 64.000 75 26 1 149 223 164 237 4.96e-25 103
MsaG002714 AT3G20910.1 48.760 121 41 5 133 239 126 239 1.49e-24 101
MsaG002714 AT3G14020.2 51.961 102 40 3 132 229 140 236 1.12e-23 99.4
MsaG002714 AT3G14020.1 51.961 102 40 3 132 229 140 236 1.12e-23 99.4
MsaG002714 AT1G72830.3 38.462 156 71 5 132 284 135 268 2.25e-21 93.2

Find 78 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGATCTCAACCTGTTTA+TGG 0.217575 1:+46090427 MsaT002714.1:CDS
TACGGGCTCTTCTCGACTTA+TGG 0.224539 1:+46091028 MsaT002714.1:CDS
AACACAAAAGTTTCTGCTAA+TGG 0.270945 1:+46091895 MsaT002714.1:CDS
CGGATCTACTGTCCACTCTT+TGG 0.272014 1:+46092128 MsaT002714.1:CDS
GTTGAACAATTGTTTGATAA+AGG 0.289283 1:+46090541 MsaT002714.1:CDS
TGACGCGATTCGTGCATATA+TGG 0.329752 1:-46091826 None:intergenic
CTGTGCAAAATATCCTTATA+TGG 0.336858 1:+46090997 MsaT002714.1:CDS
TTGGAAAGCTCCAATGAAAA+AGG 0.340104 1:-46090475 None:intergenic
ACCTCTGACCCCGAGACATT+TGG 0.360492 1:-46092054 None:intergenic
CCGTAAAATTGATCCATATA+AGG 0.372097 1:-46091010 None:intergenic
AACCAATTCACCATCTTTCC+AGG 0.379219 1:+46090571 MsaT002714.1:CDS
TCATGTTCTTTAAGATAAAC+AGG 0.380584 1:-46090337 None:intergenic
GGATCTACTGTCCACTCTTT+GGG 0.389114 1:+46092129 MsaT002714.1:CDS
TTGGGAGACATGATCGATAC+TGG 0.406979 1:+46092147 MsaT002714.1:CDS
GTATCGTCATGCCAACAAAA+TGG 0.414096 1:+46092175 MsaT002714.1:CDS
AACTTTACTGTCAATCATAT+CGG 0.418865 1:+46092108 MsaT002714.1:CDS
TACCTGGAAAGATGGTGAAT+TGG 0.421188 1:-46090573 None:intergenic
AGCAATTTGATCTATGTAGT+TGG 0.421523 1:-46090494 None:intergenic
TGCTTAGCATTCACGTATGT+TGG 0.430698 1:-46091615 None:intergenic
CAAGACTCTCCTCCATAAAC+AGG 0.453464 1:-46090439 None:intergenic
TTTATTCTGAAGCACAGCTT+TGG 0.466795 1:-46091673 None:intergenic
GATAAGGTTGCTTGATGATT+CGG 0.479300 1:-46090809 None:intergenic
GGAGGCAGTCCAAATGTCTC+GGG 0.483878 1:+46092045 MsaT002714.1:CDS
ACAAAATGGTTTGCAGCAGC+TGG 0.492247 1:+46092189 MsaT002714.1:CDS
AAACGACCACCGCATCCTCT+TGG 0.498137 1:-46091868 None:intergenic
TAAGAAGATTTACCATAGGC+TGG 0.500852 1:-46090886 None:intergenic
AAAGTTTCTGCTAATGGAAA+CGG 0.509349 1:+46091901 MsaT002714.1:CDS
CGGAGGCAGTCCAAATGTCT+CGG 0.512481 1:+46092044 MsaT002714.1:CDS
TGGAACTTTAAATTCGTCAA+AGG 0.512980 1:+46092014 MsaT002714.1:CDS
AGCAGCTGGTAGACAGCTGC+TGG 0.514662 1:+46092203 MsaT002714.1:CDS
CCATGGTGGAGTAATGCCTA+TGG 0.516782 1:+46090409 MsaT002714.1:CDS
CCTAGCTCAAAACGATTAAG+TGG 0.518018 1:-46090857 None:intergenic
AATTCGTCAAAGGAGACAAA+CGG 0.518779 1:+46092024 MsaT002714.1:CDS
TTCGGAACTCTGAGAACCAC+TGG 0.518971 1:-46091974 None:intergenic
CCGAGTAAGAACTTAGTTCG+AGG 0.522823 1:+46090517 MsaT002714.1:CDS
GCTAATGGAAACGGTAAAAG+CGG 0.528506 1:+46091910 MsaT002714.1:CDS
GGAAATGTCCACAATTCTGT+TGG 0.536104 1:+46090361 MsaT002714.1:CDS
GACTTATGGACCTCAAATCT+CGG 0.538137 1:+46091042 MsaT002714.1:CDS
CCTCGAACTAAGTTCTTACT+CGG 0.539423 1:-46090517 None:intergenic
ATAACAAGTACCTGGAAAGA+TGG 0.540599 1:-46090581 None:intergenic
CTCAAAACGATTAAGTGGCT+CGG 0.547492 1:-46090852 None:intergenic
TAAAGACAAACCGAGATTTG+AGG 0.547890 1:-46091052 None:intergenic
GGCCTACAGCTGCAGTCCAG+TGG 0.549836 1:+46091958 MsaT002714.1:CDS
GCTAGGGTTTAACCAGCCTA+TGG 0.550177 1:+46090874 MsaT002714.1:CDS
ATAAACAGGTTGAGATCCAT+AGG 0.553524 1:-46090425 None:intergenic
AACCTCAGATTGTAAGACTT+CGG 0.557052 1:-46091992 None:intergenic
TTCCGAAGTCTTACAATCTG+AGG 0.557924 1:+46091990 MsaT002714.1:CDS
AAATGATGATTGCAGTGATA+AGG 0.560313 1:-46090825 None:intergenic
TCCAAATGTCTCGGGGTCAG+AGG 0.561807 1:+46092053 MsaT002714.1:CDS
AAAACTGTCCAACAGAATTG+TGG 0.562402 1:-46090369 None:intergenic
GTTTATCTTAAAGAACATGA+AGG 0.564171 1:+46090340 MsaT002714.1:CDS
CTAATGGAAACGGTAAAAGC+GGG 0.566129 1:+46091911 MsaT002714.1:CDS
CATCTGTGACTTCAGCACCA+TGG 0.577095 1:+46090392 MsaT002714.1:CDS
GAAGTCTTACAATCTGAGGT+TGG 0.582591 1:+46091994 MsaT002714.1:CDS
TTGATGATTCGGTGTATCAA+CGG 0.586695 1:-46090798 None:intergenic
ATGCGTAGACCAAGAGGATG+CGG 0.604666 1:+46091859 MsaT002714.1:CDS
CGATCATGTCTCCCAAAGAG+TGG 0.606030 1:-46092140 None:intergenic
GTTTATGGAGGAGAGTCTTG+TGG 0.624514 1:+46090442 MsaT002714.1:CDS
TCGTCAGATGTCATGCTAAG+CGG 0.627347 1:-46091588 None:intergenic
CATGCAATGCGTAGACCAAG+AGG 0.640812 1:+46091853 MsaT002714.1:CDS
GGTGACTAGCATGTACACAC+AGG 0.643441 1:+46092074 MsaT002714.1:CDS
GGATCTCAACCTGTTTATGG+AGG 0.650456 1:+46090430 MsaT002714.1:CDS
TAAACTGATAAAGCGTAACA+AGG 0.655532 1:+46091692 MsaT002714.1:CDS
GACATGATCGATACTGGACA+CGG 0.660565 1:+46092153 MsaT002714.1:CDS
CGTAGACCAAGAGGATGCGG+TGG 0.670871 1:+46091862 MsaT002714.1:CDS
GTGAATGCTAAGCAATACCA+TGG 0.675463 1:+46091624 MsaT002714.1:CDS
CTGTGACTTCAGCACCATGG+TGG 0.675777 1:+46090395 MsaT002714.1:CDS
AACCACTGGACTGCAGCTGT+AGG 0.688049 1:-46091960 None:intergenic
ACCATGGTATCATCAGACGC+AGG 0.688525 1:+46091640 MsaT002714.1:CDS
AGTGAAGAGAACATAAACAC+TGG 0.692663 1:+46091934 MsaT002714.1:CDS
GCCTGCGTCTGATGATACCA+TGG 0.693395 1:-46091641 None:intergenic
CCATAGGCATTACTCCACCA+TGG 0.694421 1:-46090409 None:intergenic
GTGACTAGCATGTACACACA+GGG 0.698331 1:+46092075 MsaT002714.1:CDS
GAGGCAGTCCAAATGTCTCG+GGG 0.702648 1:+46092046 MsaT002714.1:CDS
GAAGAGAACATAAACACTGG+TGG 0.703232 1:+46091937 MsaT002714.1:CDS
ACTAGCATGTACACACAGGG+AGG 0.761185 1:+46092078 MsaT002714.1:CDS
CTTAGCATGACATCTGACGA+CGG 0.765545 1:+46091591 MsaT002714.1:CDS
TCGTCAAAGGAGACAAACGG+AGG 0.770276 1:+46092027 MsaT002714.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 46090325 46092233 46090325 ID=MsaG002714
Chr1 mRNA 46090325 46092233 46090325 ID=MsaT002714.1;Parent=MsaG002714
Chr1 exon 46090325 46090592 46090325 ID=MsaT002714.1.exon1;Parent=MsaT002714.1
Chr1 CDS 46090325 46090592 46090325 ID=cds.MsaT002714.1;Parent=MsaT002714.1
Chr1 exon 46090783 46090895 46090783 ID=MsaT002714.1.exon2;Parent=MsaT002714.1
Chr1 CDS 46090783 46090895 46090783 ID=cds.MsaT002714.1;Parent=MsaT002714.1
Chr1 exon 46090995 46091063 46090995 ID=MsaT002714.1.exon3;Parent=MsaT002714.1
Chr1 CDS 46090995 46091063 46090995 ID=cds.MsaT002714.1;Parent=MsaT002714.1
Chr1 exon 46091573 46091713 46091573 ID=MsaT002714.1.exon4;Parent=MsaT002714.1
Chr1 CDS 46091573 46091713 46091573 ID=cds.MsaT002714.1;Parent=MsaT002714.1
Chr1 exon 46091826 46092233 46091826 ID=MsaT002714.1.exon5;Parent=MsaT002714.1
Chr1 CDS 46091826 46092233 46091826 ID=cds.MsaT002714.1;Parent=MsaT002714.1
Gene Sequence

>MsaT002714.1

ATGGCTATGCAACCTGTTTATCTTAAAGAACATGAAGGAAATGTCCACAATTCTGTTGGACAGTTTTCATCTGTGACTTCAGCACCATGGTGGAGTAATGCCTATGGATCTCAACCTGTTTATGGAGGAGAGTCTTGTGGCCAAATGAAACCTTTTTCATTGGAGCTTTCCAACTACATAGATCAAATTGCTCCGAGTAAGAACTTAGTTCGAGGAGTTGAACAATTGTTTGATAAAGGACATACAAACCAATTCACCATCTTTCCAGATGACTGTAAGATGTCCGTTGATACACCGAATCATCAAGCAACCTTATCACTGCAATCATCATTTGCTGCCGAGCCACTTAATCGTTTTGAGCTAGGGTTTAACCAGCCTATGATCTGTGCAAAATATCCTTATATGGATCAATTTTACGGGCTCTTCTCGACTTATGGACCTCAAATCTCGGGACGTGTTATGCTTCCGCTTAGCATGACATCTGACGACGGACCAACATACGTGAATGCTAAGCAATACCATGGTATCATCAGACGCAGGCATTCTCGTGCCAAAGCTGTGCTTCAGAATAAACTGATAAAGCGTAACAAGCCATATATGCACGAATCGCGTCATCTGCATGCAATGCGTAGACCAAGAGGATGCGGTGGTCGTTTCTTGAACACAAAAGTTTCTGCTAATGGAAACGGTAAAAGCGGGAGTGAAGAGAACATAAACACTGGTGGCCTACAGCTGCAGTCCAGTGGTTCTCAGAGTTCCGAAGTCTTACAATCTGAGGTTGGAACTTTAAATTCGTCAAAGGAGACAAACGGAGGCAGTCCAAATGTCTCGGGGTCAGAGGTGACTAGCATGTACACACAGGGAGGTCTTGATAACTTTACTGTCAATCATATCGGATCTACTGTCCACTCTTTGGGAGACATGATCGATACTGGACACGGTATCGTCATGCCAACAAAATGGTTTGCAGCAGCTGGTAGACAGCTGCTGGAACCATAA
Protein sequence

>MsaT002714.1

MAMQPVYLKEHEGNVHNSVGQFSSVTSAPWWSNAYGSQPVYGGESCGQMKPFSLELSNYIDQIAPSKNLVRGVEQLFDKGHTNQFTIFPDDCKMSVDTPNHQATLSLQSSFAAEPLNRFELGFNQPMICAKYPYMDQFYGLFSTYGPQISGRVMLPLSMTSDDGPTYVNAKQYHGIIRRRHSRAKAVLQNKLIKRNKPYMHESRHLHAMRRPRGCGGRFLNTKVSANGNGKSGSEENINTGGLQLQSSGSQSSEVLQSEVGTLNSSKETNGGSPNVSGSEVTSMYTQGGLDNFTVNHIGSTVHSLGDMIDTGHGIVMPTKWFAAAGRQLLEP*