AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002722


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002722 MtrunA17_Chr1g0177151 94.340 265 15 0 1 265 1 265 0.0 509
MsaG002722 MtrunA17_Chr7g0224601 68.304 224 67 2 34 253 28 251 8.27e-107 311
MsaG002722 MtrunA17_Chr3g0099561 68.182 220 70 0 34 253 26 245 2.72e-105 307
MsaG002722 MtrunA17_Chr8g0392781 51.645 304 106 3 1 263 1 304 1.54e-97 290
MsaG002722 MtrunA17_Chr6g0477171 62.814 199 74 0 58 256 48 246 1.24e-85 256
MsaG002722 MtrunA17_Chr5g0444621 59.574 188 76 0 66 253 52 239 2.26e-77 235
MsaG002722 MtrunA17_Chr3g0083821 56.303 119 52 0 52 170 12 130 3.56e-40 135
MsaG002722 MtrunA17_Chr3g0083811 58.333 72 30 0 182 253 1 72 3.71e-23 91.3
MsaG002722 MtrunA17_Chr5g0444631 66.038 53 18 0 108 160 4 56 5.22e-17 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002722 AT3G20800.1 62.548 259 90 3 1 253 1 258 5.84e-106 310
MsaG002722 AT5G12980.1 61.176 255 96 2 1 253 1 254 8.35e-102 299
MsaG002722 AT2G32550.2 36.111 216 124 4 49 253 67 279 1.79e-37 134
MsaG002722 AT2G32550.3 36.098 205 117 4 60 253 39 240 3.81e-36 130
MsaG002722 AT2G32550.1 35.294 187 107 4 78 253 2 185 4.49e-31 115

Find 49 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCTTCATTTGCTCTCTA+AGG 0.219918 1:-46184261 MsaT002722.1:intron
CTAACTTACTTGATGTATTA+AGG 0.245270 1:+46181695 None:intergenic
TGCCTACGCAATATGGAAAT+TGG 0.348006 1:-46181441 MsaT002722.1:CDS
GCCGTTGTGCCTACGCAATA+TGG 0.352059 1:-46181448 MsaT002722.1:CDS
CAATACTCTTGCTCTACTTC+AGG 0.377796 1:-46182741 MsaT002722.1:intron
AGCAGTTAAGATGGTGGAGT+TGG 0.389913 1:+46181071 None:intergenic
AGGAAGGGATACAAATATAT+AGG 0.402029 1:+46181715 None:intergenic
GATTTAAGACTTGCCAGTCT+TGG 0.402589 1:-46181661 MsaT002722.1:CDS
ATGAATGAAAACTTTGTGTC+AGG 0.407209 1:+46182571 None:intergenic
TAAGATGGTGGAGTTGGGTT+CGG 0.419375 1:+46181077 None:intergenic
CCGTTATTGTGGAATTCAAT+TGG 0.431635 1:-46183255 MsaT002722.1:CDS
TTCTTCAAGGAGGGTGATTG+AGG 0.449123 1:+46184389 None:intergenic
TTTGTCAGATGATGATGGTT+TGG 0.451681 1:-46181252 MsaT002722.1:CDS
GAGGAAGGAAGCAGTTAAGA+TGG 0.457991 1:+46181062 None:intergenic
GTGCTGGAGGCAGATGTAAC+AGG 0.465456 1:+46184353 None:intergenic
CATCTTGTTGCCAATGGTGC+TGG 0.465484 1:+46184337 None:intergenic
CAGTTGGTCGAGCTTTGGAC+AGG 0.472419 1:-46181196 MsaT002722.1:CDS
GCAAGTCTTAAATCTTCAAA+TGG 0.486164 1:+46181670 None:intergenic
ACATCTGCCTCCAGCACCAT+TGG 0.494495 1:-46184347 MsaT002722.1:CDS
GCAGTTAAGATGGTGGAGTT+GGG 0.497274 1:+46181072 None:intergenic
AAGAACTCGCACCGTTATTG+TGG 0.506453 1:-46183266 MsaT002722.1:CDS
CTTACTTGATGTATTAAGGA+AGG 0.509839 1:+46181699 None:intergenic
TCTTCAAGGAGGGTGATTGA+GGG 0.516540 1:+46184390 None:intergenic
CATATTCGAGTTGATTGTGT+AGG 0.521680 1:+46182764 None:intergenic
CCAATTGAATTCCACAATAA+CGG 0.522825 1:+46183255 None:intergenic
GGCAGCAATGACTCCAAGAC+TGG 0.526684 1:+46181648 None:intergenic
TCGAGCTTTGGACAGGATGT+TGG 0.529344 1:-46181189 MsaT002722.1:CDS
ATATTCACAGGTTAAGACTA+AGG 0.533680 1:-46181505 MsaT002722.1:intron
TTACTTGATGTATTAAGGAA+GGG 0.537664 1:+46181700 None:intergenic
AGTCATTGCTGCCATGGTAA+AGG 0.543352 1:-46181638 MsaT002722.1:intron
GAAGGAAGCAGTTAAGATGG+TGG 0.550389 1:+46181065 None:intergenic
TCTTGGAGTCATTGCTGCCA+TGG 0.555856 1:-46181644 MsaT002722.1:CDS
AATTCATTATTCTTCAAGGA+GGG 0.558130 1:+46184380 None:intergenic
TTCAAGGAGGGTGATTGAGG+GGG 0.561905 1:+46184392 None:intergenic
AGCTTTAAAAGACGAGGTGA+AGG 0.570450 1:+46181149 None:intergenic
TCAAGGAGGGTGATTGAGGG+GGG 0.591467 1:+46184393 None:intergenic
GCATTACTTCAGTTGCGAGA+AGG 0.595531 1:+46181469 None:intergenic
TGAATGAAAACTTTGTGTCA+GGG 0.602907 1:+46182572 None:intergenic
TGCCAATTTCCATATTGCGT+AGG 0.604311 1:+46181439 None:intergenic
ATACAACATCTTGTTGCCAA+TGG 0.605197 1:+46184331 None:intergenic
CTTGTTGCCAATGGTGCTGG+AGG 0.622571 1:+46184340 None:intergenic
TCCATATTGCGTAGGCACAA+CGG 0.628181 1:+46181447 None:intergenic
GTTAAGACTAAGGAAGCGAT+AGG 0.628788 1:-46181495 MsaT002722.1:CDS
AAATTCATTATTCTTCAAGG+AGG 0.632490 1:+46184379 None:intergenic
CTTCAAGGAGGGTGATTGAG+GGG 0.647804 1:+46184391 None:intergenic
ATCATAAGCTTTAAAAGACG+AGG 0.660763 1:+46181143 None:intergenic
TTTGTGAGACTTGTATAACA+CGG 0.661280 1:+46181119 None:intergenic
TGAAAACTTTGTGTCAGGGT+GGG 0.675917 1:+46182576 None:intergenic
ATGAAAACTTTGTGTCAGGG+TGG 0.707370 1:+46182575 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 46181076 46184426 46181076 ID=MsaG002722
Chr1 mRNA 46181076 46184426 46181076 ID=MsaT002722.1;Parent=MsaG002722
Chr1 exon 46181076 46181300 46181076 ID=MsaT002722.1.exon7;Parent=MsaT002722.1
Chr1 CDS 46181076 46181300 46181076 ID=cds.MsaT002722.1;Parent=MsaT002722.1
Chr1 exon 46181422 46181517 46181422 ID=MsaT002722.1.exon6;Parent=MsaT002722.1
Chr1 CDS 46181422 46181517 46181422 ID=cds.MsaT002722.1;Parent=MsaT002722.1
Chr1 exon 46181639 46181745 46181639 ID=MsaT002722.1.exon5;Parent=MsaT002722.1
Chr1 CDS 46181639 46181745 46181639 ID=cds.MsaT002722.1;Parent=MsaT002722.1
Chr1 exon 46182566 46182608 46182566 ID=MsaT002722.1.exon4;Parent=MsaT002722.1
Chr1 CDS 46182566 46182608 46182566 ID=cds.MsaT002722.1;Parent=MsaT002722.1
Chr1 exon 46182742 46182831 46182742 ID=MsaT002722.1.exon3;Parent=MsaT002722.1
Chr1 CDS 46182742 46182831 46182742 ID=cds.MsaT002722.1;Parent=MsaT002722.1
Chr1 exon 46183233 46183304 46183233 ID=MsaT002722.1.exon2;Parent=MsaT002722.1
Chr1 CDS 46183233 46183304 46183233 ID=cds.MsaT002722.1;Parent=MsaT002722.1
Chr1 exon 46184262 46184426 46184262 ID=MsaT002722.1.exon1;Parent=MsaT002722.1
Chr1 CDS 46184262 46184426 46184262 ID=cds.MsaT002722.1;Parent=MsaT002722.1
Gene Sequence

>MsaT002722.1

ATGGCGAATTTCCCCCCTCAATCACCCTCCTTGAAGAATAATGAATTTTTTCCTGTTACATCTGCCTCCAGCACCATTGGCAACAAGATGTTGTATGCTGAGCGTCTTGTTATTGAACTGAGCAACCCCGATCTACGAGAAAATGCTCTTCATTTGCTCTCTAAGATGACAGATTTGTTTCAAGAACTCGCACCGTTATTGTGGAATTCAATTGGTACTATTGCAATATTTTTACAGGAAATAATTACAATATATCCTGCTCTTTCACCAGAAAATCTTACTCCTACACAATCAACTCGAATATGCAATACTCTTGCTCTACTTCAGTGTGTGGCTTCCCACCCTGACACAAAGTTTTCATTCATGAAAGCTAACATACCTATATATTTGTATCCCTTCCTTAATACATCAAGTAAGTTAGCTCCATTTGAAGATTTAAGACTTGCCAGTCTTGGAGTCATTGCTGCCATGGTAAAGGTTAAGACTAAGGAAGCGATAGGCTTCCTTCTCGCAACTGAAGTAATGCCGTTGTGCCTACGCAATATGGAAATTGGCAAAGAATTGTCAAAAACTGTTGCAACATTTATCGTTGAGAAAATTTTGTCAGATGATGATGGTTTGGCGTATGTTTGTGCTACACCAGATCGTTTTTTTGCAGTTGGTCGAGCTTTGGACAGGATGTTGGCAAGTGTTGACAATCAACCTTCACCTCGTCTTTTAAAGCTTATGATTCCGTGTTATACAAGTCTCACAAAAAATGTAGGGTTGTTTAAGCCGAACCCAACTCCACCATCTTAA

Protein sequence

>MsaT002722.1

MANFPPQSPSLKNNEFFPVTSASSTIGNKMLYAERLVIELSNPDLRENALHLLSKMTDLFQELAPLLWNSIGTIAIFLQEIITIYPALSPENLTPTQSTRICNTLALLQCVASHPDTKFSFMKANIPIYLYPFLNTSSKLAPFEDLRLASLGVIAAMVKVKTKEAIGFLLATEVMPLCLRNMEIGKELSKTVATFIVEKILSDDDGLAYVCATPDRFFAVGRALDRMLASVDNQPSPRLLKLMIPCYTSLTKNVGLFKPNPTPPS*