Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002791 | XP_013467912.2 | 93.913 | 230 | 14 | 0 | 1 | 230 | 1 | 230 | 3.54e-130 | 377 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002791 | sp|Q9FMC8|OPF13_ARATH | 33.597 | 253 | 138 | 9 | 3 | 236 | 4 | 245 | 2.89e-28 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002791 | tr|A0A396JMH3|A0A396JMH3_MEDTR | 93.913 | 230 | 14 | 0 | 1 | 230 | 1 | 230 | 1.69e-130 | 377 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG002791 | TF | OFP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002791 | MtrunA17_Chr1g0177911 | 93.913 | 230 | 14 | 0 | 1 | 230 | 1 | 230 | 3.25e-134 | 377 |
| MsaG002791 | MtrunA17_Chr7g0267101 | 46.269 | 201 | 92 | 7 | 3 | 195 | 5 | 197 | 1.46e-44 | 151 |
| MsaG002791 | MtrunA17_Chr1g0210351 | 40.284 | 211 | 93 | 6 | 3 | 195 | 4 | 199 | 8.25e-41 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002791 | AT5G04820.1 | 33.597 | 253 | 138 | 9 | 3 | 236 | 4 | 245 | 2.94e-29 | 110 |
| MsaG002791 | AT2G36050.1 | 36.416 | 173 | 76 | 8 | 27 | 195 | 29 | 171 | 7.69e-15 | 72.4 |
| MsaG002791 | AT3G52540.1 | 39.316 | 117 | 56 | 5 | 82 | 195 | 94 | 198 | 3.00e-11 | 62.4 |
Find 35 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAATATTAATTATTTCTT+TGG | 0.213180 | 1:+47590281 | None:intergenic |
| ATAAGAAAATTCAGTTAATA+TGG | 0.287857 | 1:+47590410 | None:intergenic |
| GAACAACTATGAATAGAAAA+AGG | 0.332701 | 1:+47589988 | None:intergenic |
| ACCTACAAAGGTTGGGTTGA+TGG | 0.345047 | 1:+47590467 | None:intergenic |
| CAAAGAATCTATTGTGGAAA+TGG | 0.346941 | 1:-47590160 | MsaT002791.1:CDS |
| ATGGAAAATCCTCTTCTTCA+TGG | 0.370442 | 1:-47590560 | MsaT002791.1:CDS |
| ATATGGAGTCAGAAGTTTCT+AGG | 0.382955 | 1:+47590427 | None:intergenic |
| TCCATCAACCCAACCTTTGT+AGG | 0.396189 | 1:-47590468 | MsaT002791.1:CDS |
| AATCCTCTTCTTCATGGTCT+TGG | 0.397416 | 1:-47590554 | MsaT002791.1:CDS |
| AATATCGAGCTTGTTTCATT+CGG | 0.425339 | 1:+47590309 | None:intergenic |
| AGTTTCTAGGATATCTTTGT+TGG | 0.429909 | 1:+47590440 | None:intergenic |
| AATGATTTGCGGTTTGTGTT+CGG | 0.436168 | 1:-47590349 | MsaT002791.1:CDS |
| GAGTATTTCAAAGGTAATAG+TGG | 0.437931 | 1:-47590387 | MsaT002791.1:CDS |
| GGCTCATGATATTAACGATT+GGG | 0.445267 | 1:-47590133 | MsaT002791.1:CDS |
| TAACAACTTATTATTACCAT+TGG | 0.449491 | 1:-47590232 | MsaT002791.1:CDS |
| TTCTAGGATATCTTTGTTGG+TGG | 0.454097 | 1:+47590443 | None:intergenic |
| GTTCTTCTCTTGATTCATTG+TGG | 0.463886 | 1:-47589969 | MsaT002791.1:CDS |
| GTTAATGAGAAAAGAAATCA+TGG | 0.484456 | 1:-47590075 | MsaT002791.1:CDS |
| TTGGTGGAACCTACAAAGGT+TGG | 0.485113 | 1:+47590459 | None:intergenic |
| TGGGTTGATGGATTTGAATA+CGG | 0.486750 | 1:+47590479 | None:intergenic |
| TCAATAGAGACAATGATTTG+CGG | 0.506903 | 1:-47590360 | MsaT002791.1:CDS |
| GCGAATCCATTGAAAACTCA+AGG | 0.518597 | 1:+47590202 | None:intergenic |
| TTTGCGGTTTGTGTTCGGAT+AGG | 0.524474 | 1:-47590344 | MsaT002791.1:CDS |
| TGGTGGAACCTACAAAGGTT+GGG | 0.527801 | 1:+47590460 | None:intergenic |
| ATCTATTGTGGAAATGGTGG+AGG | 0.538393 | 1:-47590154 | MsaT002791.1:CDS |
| TTTGTTGGTGGAACCTACAA+AGG | 0.547175 | 1:+47590455 | None:intergenic |
| AGGCTCATGATATTAACGAT+TGG | 0.552786 | 1:-47590134 | MsaT002791.1:CDS |
| AACTCAAGGGTGTTCTCCAA+TGG | 0.557424 | 1:+47590216 | None:intergenic |
| AGAATCTATTGTGGAAATGG+TGG | 0.559217 | 1:-47590157 | MsaT002791.1:CDS |
| TTTCATCTTATAGTTCACCT+TGG | 0.576082 | 1:-47589933 | MsaT002791.1:intron |
| TGGCCAAGACCATGAAGAAG+AGG | 0.591369 | 1:+47590551 | None:intergenic |
| AGATTTCAAAGAATCTATTG+TGG | 0.610205 | 1:-47590166 | MsaT002791.1:CDS |
| CGAATCCATTGAAAACTCAA+GGG | 0.622493 | 1:+47590203 | None:intergenic |
| GATTCTTTGAAATCTTCGCA+AGG | 0.641294 | 1:+47590174 | None:intergenic |
| GAACTATAAGATGAAATACA+AGG | 0.649755 | 1:+47589940 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 47589669 | 47590625 | 47589669 | ID=MsaG002791 |
| Chr1 | mRNA | 47589669 | 47590625 | 47589669 | ID=MsaT002791.1;Parent=MsaG002791 |
| Chr1 | exon | 47589669 | 47589705 | 47589669 | ID=MsaT002791.1.exon2;Parent=MsaT002791.1 |
| Chr1 | CDS | 47589669 | 47589705 | 47589669 | ID=cds.MsaT002791.1;Parent=MsaT002791.1 |
| Chr1 | exon | 47589934 | 47590625 | 47589934 | ID=MsaT002791.1.exon1;Parent=MsaT002791.1 |
| Chr1 | CDS | 47589934 | 47590625 | 47589934 | ID=cds.MsaT002791.1;Parent=MsaT002791.1 |
| Gene Sequence |
| Protein sequence |
