Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002794 | KEH41952.1 | 92.308 | 91 | 5 | 1 | 1 | 89 | 1 | 91 | 5.95e-54 | 172 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG002794 | tr|A0A072VJI7|A0A072VJI7_MEDTR | 92.308 | 91 | 5 | 1 | 1 | 89 | 1 | 91 | 2.84e-54 | 172 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG002794 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG002793 | MsaG002794 | 0.878481 | 5.688175e-69 | 9.570115e-66 |
| MsaG002794 | MsaG002795 | 0.874782 | 1.067532e-67 | 1.520765e-64 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAGTGATCATTTCTTTCA+TGG | 0.293801 | 1:-47620676 | None:intergenic |
| AGGAGGAGAAGAAATTGATT+TGG | 0.338250 | 1:+47620752 | MsaT002794.1:CDS |
| TCTCCTCCTTGACTTGGACT+TGG | 0.356989 | 1:-47620738 | None:intergenic |
| ATTTCTTCTCCTCCTTGACT+TGG | 0.410406 | 1:-47620744 | None:intergenic |
| TCCTTGACTTGGACTTGGAT+TGG | 0.422024 | 1:-47620733 | None:intergenic |
| GACTCTCATGTGACCATTCA+AGG | 0.426545 | 1:-47620631 | None:intergenic |
| AGACTTATAACTTGAGGTCT+AGG | 0.432547 | 1:-47620821 | None:intergenic |
| TCAAGAGTGAATGCCTTGAA+TGG | 0.452902 | 1:+47620618 | MsaT002794.1:CDS |
| GTGCTAGGAGTAGCCTTGGT+TGG | 0.456915 | 1:-47620789 | None:intergenic |
| GGCTACTCCTAGCACTAAGT+TGG | 0.507989 | 1:+47620797 | MsaT002794.1:CDS |
| TTTGGTTCTTCGCTTCATGT+TGG | 0.519903 | 1:-47620704 | None:intergenic |
| CTTCATAGACTTATAACTTG+AGG | 0.541664 | 1:-47620827 | None:intergenic |
| CTTAGTGCTAGGAGTAGCCT+TGG | 0.543078 | 1:-47620793 | None:intergenic |
| ATGAATTTCCCTATGATCCA+TGG | 0.571015 | 1:-47620568 | None:intergenic |
| ATTGATTTGGAACTAAGACT+TGG | 0.574413 | 1:+47620765 | MsaT002794.1:CDS |
| TCTAGGTCCAACTTAGTGCT+AGG | 0.576797 | 1:-47620804 | None:intergenic |
| ATGGTCACATGAGAGTCCAC+AGG | 0.587507 | 1:+47620637 | MsaT002794.1:CDS |
| ACCAATCCAAGTCCAAGTCA+AGG | 0.611700 | 1:+47620732 | MsaT002794.1:CDS |
| TGGTCACATGAGAGTCCACA+GGG | 0.650216 | 1:+47620638 | MsaT002794.1:CDS |
| ACTAAGACTTGGTCCAACCA+AGG | 0.676612 | 1:+47620776 | MsaT002794.1:CDS |
| AATCCAAGTCCAAGTCAAGG+AGG | 0.729846 | 1:+47620735 | MsaT002794.1:CDS |
| TCACATGAGAGTCCACAGGG+AGG | 0.780305 | 1:+47620641 | MsaT002794.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 47620570 | 47620839 | 47620570 | ID=MsaG002794 |
| Chr1 | mRNA | 47620570 | 47620839 | 47620570 | ID=MsaT002794.1;Parent=MsaG002794 |
| Chr1 | exon | 47620570 | 47620839 | 47620570 | ID=MsaT002794.1.exon1;Parent=MsaT002794.1 |
| Chr1 | CDS | 47620570 | 47620839 | 47620570 | ID=cds.MsaT002794.1;Parent=MsaT002794.1 |
| Gene Sequence |
| Protein sequence |
