AlfalfaGEDB | MsaG002876

M. sativa ssp. caerulea / MsaG002876

KEGG: -
GO: GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002876	KEH42071.1	93.930	313	15	3	1	312	178	487	0.0	595

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002876	sp\|Q9SA82\|BH052_ARATH	41.520	171	97	2	135	305	95	262	3.25e-28	112

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002876	tr\|A0A072VKB3\|A0A072VKB3_MEDTR	93.930	313	15	3	1	312	178	487	0.0	595

TFs/TRs:

Gene ID	Type	Classification
MsaG002876	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002876	MtrunA17_Chr1g0179061	93.802	242	12	2	1	241	1	240	1.89e-166	462
MsaG002876	MtrunA17_Chr2g0311101	81.056	322	27	10	1	312	13	310	1.58e-161	452
MsaG002876	MtrunA17_Chr8g0384141	58.491	53	22	0	182	234	125	177	2.02e-13	69.3
MsaG002876	MtrunA17_Chr3g0134001	58.491	53	22	0	182	234	182	234	1.42e-12	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG002876	AT1G30670.1	41.520	171	97	2	135	305	95	262	3.30e-29	112
MsaG002876	AT2G34820.1	42.254	213	115	4	97	305	81	289	2.65e-28	111
MsaG002876	AT3G21330.1	60.377	53	21	0	182	234	280	332	1.93e-13	70.5
MsaG002876	AT5G01305.1	50.000	58	29	0	182	239	48	105	1.32e-12	65.5
MsaG002876	AT4G00120.1	52.830	53	25	0	182	234	123	175	6.33e-12	63.9

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTTGGAATGCTTCAACTTA+TGG	0.195386	1:-48645479	MsaT002876.1:CDS
ATCTCTGCTGTGTTCATCTT+TGG	0.238213	1:+48645544	None:intergenic
TTGGTGAAATCTTCCTCATT+TGG	0.290806	1:+48645865	None:intergenic
ATCAAAACCTACCATTCTTA+AGG	0.300143	1:-48644449	MsaT002876.1:CDS
TCAAATAGTTAGAGGGAAAA+TGG	0.318116	1:+48646040	None:intergenic
AAATAAAACTATTCTTGCTT+TGG	0.325467	1:+48644388	None:intergenic
TGGTGAAATCTTCCTCATTT+GGG	0.332786	1:+48645866	None:intergenic
GAAGCAAAAGGGTTAGGGTT+AGG	0.345524	1:+48645730	None:intergenic
AAGCAAAAGGGTTAGGGTTA+GGG	0.345934	1:+48645731	None:intergenic
TCTTCTCGTAGCATAGCTTT+TGG	0.365802	1:+48645821	None:intergenic
AGTTGATCTTGACAAGGGTT+AGG	0.369040	1:+48645949	None:intergenic
TCAAAGGAAGCAAAAGGGTT+AGG	0.369513	1:+48645724	None:intergenic
AGTAGTGATTTCTAAGTTGT+TGG	0.372241	1:+48646104	None:intergenic
GTAGTTTGAATAGGTTGATA+AGG	0.378786	1:+48646126	None:intergenic
TACAGTTTCTCTTGAACAAA+TGG	0.383908	1:+48644538	None:intergenic
AAGGGTCTGAAACAGCTTGC+TGG	0.388228	1:-48644430	MsaT002876.1:CDS
AATGTGGTGACAAACTCTTT+TGG	0.390869	1:+48644493	None:intergenic
GAGCTTGGGAAGTTGGTTCC+TGG	0.392352	1:-48645571	MsaT002876.1:CDS
GACACAAGAGCTTGGGAAGT+TGG	0.397999	1:-48645578	MsaT002876.1:CDS
AAGTTGTTGGTAGTTTGAAT+AGG	0.402079	1:+48646117	None:intergenic
TTGATTATTTCAGGAAGAAA+AGG	0.406675	1:-48644602	MsaT002876.1:intron
TAGTTTGAATAGGTTGATAA+GGG	0.429054	1:+48646127	None:intergenic
AGTTTCATTCAAATAGTTAG+AGG	0.435549	1:+48646032	None:intergenic
GTTGATCTTGACAAGGGTTA+GGG	0.453473	1:+48645950	None:intergenic
TCAAAACCTACCATTCTTAA+GGG	0.456430	1:-48644448	MsaT002876.1:CDS
CAAAGGAAGCAAAAGGGTTA+GGG	0.461232	1:+48645725	None:intergenic
AGAGTATCGCGGCGAGAGAA+AGG	0.466876	1:-48645621	MsaT002876.1:CDS
TCTACCTCAAAGGAAGCAAA+AGG	0.468900	1:+48645718	None:intergenic
TTGTGTAGATCTTCACTTGG+AGG	0.473071	1:+48644574	None:intergenic
GTTCAATGTGAGAAGAAAGT+TGG	0.488552	1:-48645664	MsaT002876.1:CDS
TGTAGATCTTCACTTGGAGG+AGG	0.494574	1:+48644577	None:intergenic
GTGTTCATCTTTGGTCCACC+AGG	0.499817	1:+48645553	None:intergenic
ACTGAGAAGACACAAGAGCT+TGG	0.499993	1:-48645586	MsaT002876.1:CDS
CTGAGAAGACACAAGAGCTT+GGG	0.500383	1:-48645585	MsaT002876.1:CDS
CTGTTTCAGACCCTTAAGAA+TGG	0.516519	1:+48644438	None:intergenic
AGGGTCTGAAACAGCTTGCT+GGG	0.522554	1:-48644429	MsaT002876.1:CDS
GTTTCATTCAAATAGTTAGA+GGG	0.549802	1:+48646033	None:intergenic
CTTGGGAAGTTGGTTCCTGG+TGG	0.551828	1:-48645568	MsaT002876.1:CDS
GTGAGAAGAAAGTTGGTGAA+AGG	0.566992	1:-48645657	MsaT002876.1:CDS
AAGTTTCTTCAAGCTCAAGT+TGG	0.567049	1:-48645496	MsaT002876.1:CDS
AGCAAAGGGTTCTACCTCAA+AGG	0.572532	1:+48645708	None:intergenic
AGTTTGTGTAGATCTTCACT+TGG	0.582386	1:+48644571	None:intergenic
TTCAGACCCTTAAGAATGGT+AGG	0.583734	1:+48644442	None:intergenic
GAGTTCAAAAGCTCTTGTTG+TGG	0.586358	1:+48645787	None:intergenic
TCGCGGCGAGAGAAAGGAGA+AGG	0.587504	1:-48645615	MsaT002876.1:CDS
ACTTATGGAAACATTTAACA+AGG	0.595324	1:-48645464	MsaT002876.1:intron
CTACCTCAAAGGAAGCAAAA+GGG	0.620756	1:+48645719	None:intergenic
AATAGTTAGAGGGAAAATGG+TGG	0.625963	1:+48646043	None:intergenic
ACAAGTAGTTGATCTTGACA+AGG	0.633389	1:+48645943	None:intergenic
CAAGTAGTTGATCTTGACAA+GGG	0.656926	1:+48645944	None:intergenic
ATGCTACGAGAAGAAAAGAG+AGG	0.663245	1:-48645812	MsaT002876.1:CDS
ATCATTATGATTGCTCAATG+TGG	0.666694	1:+48644477	None:intergenic
TATCTCGTCGCAGAGTATCG+CGG	0.686396	1:-48645632	MsaT002876.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	48644396	48646187	48644396	ID=MsaG002876
Chr1	mRNA	48644396	48646187	48644396	ID=MsaT002876.1;Parent=MsaG002876
Chr1	exon	48644396	48644611	48644396	ID=MsaT002876.1.exon2;Parent=MsaT002876.1
Chr1	CDS	48644396	48644611	48644396	ID=cds.MsaT002876.1;Parent=MsaT002876.1
Chr1	exon	48645465	48646187	48645465	ID=MsaT002876.1.exon1;Parent=MsaT002876.1
Chr1	CDS	48645465	48646187	48645465	ID=cds.MsaT002876.1;Parent=MsaT002876.1

Gene Sequence

>MsaT002876.1

ATGGGAGCACTTAGCACATACTTCAACTATAATTGTTACCCTTATCAACCTATTCAAACTACCAACAACTTAGAAATCACTACTTTTCAACATCATGAACAAGAACAATTTGTGCAACAAAACCACCATTTTCCCTCTAACTATTTGAATGAAACTTCTCATTTAGATGAAAGCTTCATTTTTCAAACTCCTTGTTTTTACTCAAATGAAACTTACCCTAACCCTTGTCAAGATCAACTACTTGTTGACTCCACTTTTTCTTCTCAAAATGATGGCTTTGTTTCAATGAATGAAATTTTCCCAAATGAGGAAGATTTCACCAATTACCTTACATGTCCAAAACGCCAAAAGCTATGCTACGAGAAGAAAAGAGAGGAACCACAACAAGAGCTTTTGAACTCAACCAATTTTTTTGTGGATGAGTTTATGACTAACCCTAACCCTAACCCTTTTGCTTCCTTTGAGGTAGAACCCTTTGCTGCTTCAAAAATTGTTGATGTTGTTCAATGTGAGAAGAAAGTTGGTGAAAGGACTATCTCGTCGCAGAGTATCGCGGCGAGAGAAAGGAGAAGGAAGATAACTGAGAAGACACAAGAGCTTGGGAAGTTGGTTCCTGGTGGACCAAAGATGAACACAGCAGAGATGCTTAATGCTGCTGCTAATTATGTCAAGTTTCTTCAAGCTCAAGTTGGAATGCTTCAACTTATGGAAACATTTAACAAGGAAGAAAAGGAACCTCCTCCAAGTGAAGATCTACACAAACTTCTTCTTTCTCCATTTGTTCAAGAGAAACTGTATTCAGAAGAAAAGTGCTTTGTTCCAAAAGAGTTTGTCACCACATTGAGCAATCATAATGATGTTCAATCAAAACCTACCATTCTTAAGGGTCTGAAACAGCTTGCTGGGACTGAGATTGAGAAGAAACCAAAGCAAGAATAG

Protein sequence

>MsaT002876.1

MGALSTYFNYNCYPYQPIQTTNNLEITTFQHHEQEQFVQQNHHFPSNYLNETSHLDESFIFQTPCFYSNETYPNPCQDQLLVDSTFSSQNDGFVSMNEIFPNEEDFTNYLTCPKRQKLCYEKKREEPQQELLNSTNFFVDEFMTNPNPNPFASFEVEPFAASKIVDVVQCEKKVGERTISSQSIAARERRRKITEKTQELGKLVPGGPKMNTAEMLNAAANYVKFLQAQVGMLQLMETFNKEEKEPPPSEDLHKLLLSPFVQEKLYSEEKCFVPKEFVTTLSNHNDVQSKPTILKGLKQLAGTEIEKKPKQE*