AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002920 MtrunA17_Chr7g0265521 56.327 245 82 11 1 233 1 232 3.46e-77 232
MsaG002920 MtrunA17_Chr1g0179581 98.198 111 1 1 32 142 1 110 5.16e-73 217
MsaG002920 MtrunA17_Chr1g0179591 68.182 132 37 1 102 233 3 129 2.90e-62 191
MsaG002920 MtrunA17_Chr6g0450991 56.863 102 37 1 59 153 66 167 3.45e-30 113
MsaG002920 MtrunA17_Chr4g0060031 50.420 119 52 1 49 160 84 202 1.30e-29 112
MsaG002920 MtrunA17_Chr6g0453021 55.670 97 43 0 54 150 90 186 3.26e-28 108
MsaG002920 MtrunA17_Chr8g0362151 55.670 97 36 1 57 146 74 170 6.60e-28 107
MsaG002920 MtrunA17_Chr3g0123691 38.202 178 88 5 55 221 55 221 3.50e-27 105
MsaG002920 MtrunA17_Chr8g0381741 40.645 155 79 4 48 189 39 193 1.71e-26 104
MsaG002920 MtrunA17_Chr5g0415941 54.128 109 39 2 58 155 85 193 2.35e-26 103
MsaG002920 MtrunA17_Chr5g0416391 56.180 89 39 0 59 147 76 164 2.75e-26 103
MsaG002920 MtrunA17_Chr3g0127511 49.038 104 45 2 62 158 40 142 2.72e-25 99.8
MsaG002920 MtrunA17_Chr8g0362751 53.608 97 38 1 60 149 89 185 3.12e-25 100
MsaG002920 MtrunA17_Chr3g0119291 41.722 151 79 3 59 207 58 201 4.37e-25 99.4
MsaG002920 MtrunA17_Chr7g0217111 54.639 97 37 1 58 147 78 174 2.60e-24 97.4
MsaG002920 MtrunA17_Chr8g0346251 42.308 130 56 3 64 183 45 165 4.72e-24 96.3
MsaG002920 MtrunA17_Chr5g0403701 44.340 106 59 0 55 160 48 153 8.29e-21 86.7
MsaG002920 MtrunA17_Chr4g0054421 48.315 89 46 0 60 148 127 215 1.90e-18 81.6
MsaG002920 MtrunA17_Chr7g0258831 45.161 93 51 0 60 152 160 252 2.48e-18 82.0
MsaG002920 MtrunA17_Chr5g0403691 42.478 113 58 1 55 160 48 160 2.58e-18 80.5
MsaG002920 MtrunA17_Chr4g0055851 38.971 136 66 3 60 187 120 246 2.69e-18 81.6
MsaG002920 MtrunA17_Chr1g0151971 43.119 109 55 2 47 148 110 218 1.37e-17 79.7
MsaG002920 MtrunA17_Chr1g0175651 44.944 89 49 0 60 148 154 242 1.89e-17 79.3
MsaG002920 MtrunA17_Chr5g0399491 47.191 89 47 0 60 148 136 224 6.81e-17 77.8
MsaG002920 MtrunA17_Chr8g0335131 46.067 89 48 0 60 148 146 234 9.31e-17 77.8
MsaG002920 MtrunA17_Chr2g0308611 43.820 89 50 0 60 148 200 288 1.29e-16 77.4
MsaG002920 MtrunA17_Chr4g0044091 44.681 94 52 0 55 148 60 153 1.87e-16 75.1
MsaG002920 MtrunA17_Chr5g0400781 43.820 89 50 0 60 148 123 211 2.18e-15 73.2
MsaG002920 MtrunA17_Chr4g0071411 44.186 86 48 0 63 148 91 176 2.76e-15 72.4
MsaG002920 MtrunA17_Chr3g0135331 43.820 89 50 0 60 148 145 233 1.23e-14 71.6
MsaG002920 MtrunA17_Chr2g0299031 42.697 89 51 0 60 148 82 170 3.91e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002920 AT5G03790.1 50.769 195 78 6 1 178 1 194 4.02e-49 161
MsaG002920 AT5G03790.2 53.704 162 58 5 1 145 1 162 1.27e-42 143
MsaG002920 AT2G36610.1 49.162 179 65 7 7 162 5 180 1.79e-41 140
MsaG002920 AT4G40060.1 41.875 160 82 3 27 177 28 185 1.08e-31 117
MsaG002920 AT1G69780.1 44.755 143 66 1 57 199 82 211 1.27e-30 115
MsaG002920 AT5G15150.1 62.366 93 35 0 57 149 112 204 1.01e-29 113
MsaG002920 AT2G22430.1 54.455 101 39 1 56 149 58 158 1.02e-28 110
MsaG002920 AT5G65310.1 41.667 144 72 4 12 151 28 163 5.42e-28 108
MsaG002920 AT5G65310.2 41.667 144 72 4 12 151 10 145 5.57e-28 108
MsaG002920 AT3G01220.1 53.211 109 44 1 57 158 84 192 1.88e-27 106
MsaG002920 AT3G01470.1 38.562 153 84 2 57 202 65 214 4.13e-27 105
MsaG002920 AT2G46680.1 49.541 109 48 1 45 146 17 125 5.15e-27 104
MsaG002920 AT2G46680.2 47.826 115 53 1 45 152 17 131 3.21e-26 102
MsaG002920 AT3G61890.1 38.650 163 71 3 63 214 33 177 3.71e-26 102
MsaG002920 AT1G26960.1 42.958 142 60 3 57 193 68 193 4.09e-25 99.8
MsaG002920 AT2G18550.1 40.476 126 71 1 55 176 56 181 1.40e-24 97.4
MsaG002920 AT4G36740.1 50.526 95 47 0 55 149 50 144 3.20e-24 96.3
MsaG002920 AT4G36740.2 50.526 95 47 0 55 149 51 145 3.34e-24 96.3
MsaG002920 AT1G27045.5 49.020 102 45 1 60 154 25 126 1.69e-22 90.9
MsaG002920 AT1G27045.3 49.020 102 45 1 60 154 25 126 1.69e-22 90.9
MsaG002920 AT1G27045.1 49.020 102 45 1 60 154 68 169 1.78e-22 92.0
MsaG002920 AT1G27045.2 49.020 102 45 1 60 154 17 118 2.00e-22 90.5
MsaG002920 AT5G66700.1 48.913 92 47 0 60 151 71 162 1.20e-21 89.7
MsaG002920 AT1G27045.4 43.636 110 58 1 60 169 68 173 8.31e-21 88.2
MsaG002920 AT5G53980.1 44.211 95 53 0 60 154 11 105 3.09e-17 76.3
MsaG002920 AT4G16780.1 43.617 94 53 0 55 148 124 217 4.53e-17 78.6
MsaG002920 AT5G06710.1 44.944 89 49 0 60 148 190 278 1.35e-16 77.8
MsaG002920 AT2G01430.1 48.315 89 46 0 60 148 139 227 2.69e-16 76.3
MsaG002920 AT4G17460.1 41.489 94 55 0 55 148 130 223 3.29e-16 76.3
MsaG002920 AT3G60390.1 46.067 89 48 0 60 148 162 250 3.39e-16 76.6
MsaG002920 AT2G22800.1 42.553 94 54 0 55 148 108 201 9.02e-16 74.7
MsaG002920 AT5G47370.1 31.973 147 91 2 11 148 72 218 1.52e-15 74.3
MsaG002920 AT4G37790.1 42.553 94 54 0 55 148 121 214 1.59e-15 74.3
MsaG002920 AT2G44910.1 42.697 89 51 0 60 148 163 251 3.89e-15 73.6
MsaG002920 AT1G70920.2 41.489 94 55 0 55 148 34 127 2.12e-12 63.9
MsaG002920 AT1G70920.1 41.489 94 55 0 55 148 64 157 2.33e-12 64.3
MsaG002920 AT5G06710.4 53.846 52 24 0 60 111 190 241 9.90e-12 63.5
MsaG002920 AT2G01430.2 56.604 53 23 0 60 112 139 191 2.10e-11 61.6

Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACCTAGCACGCCTATTT+TGG 0.156053 1:-49312239 None:intergenic
TTGCTATTTGGTTCCAAAAT+AGG 0.158232 1:+49312226 MsaT002920.1:CDS
AGCCTCGTCAAATTGCTATT+TGG 0.235729 1:+49312214 MsaT002920.1:CDS
CCTTGGACACAACCTCAAAT+TGG 0.274196 1:-49312302 None:intergenic
ATGAAGCTTTCCAAGGAGTT+AGG 0.302805 1:+49312186 MsaT002920.1:CDS
AAACTGTTGTTTCAATTTAG+AGG 0.320208 1:+49314158 MsaT002920.1:CDS
CTTTCCCTTCACCTCTCTGT+TGG 0.348637 1:-49314106 None:intergenic
GAAAGCTTCATCTTCCTTTG+AGG 0.362313 1:-49312174 None:intergenic
AATTAAGGGTGATAGTTATA+AGG 0.365223 1:-49314234 None:intergenic
AGATACAATGTTCCCAGAAA+TGG 0.367312 1:+49312047 MsaT002920.1:CDS
AAAGCTTCATCTTCCTTTGA+GGG 0.383872 1:-49312173 None:intergenic
GCAGGCCAGTAGGGCAATAG+TGG 0.385106 1:-49314204 None:intergenic
GTAGTTGTAGAGGAAGCTTA+AGG 0.434188 1:-49311617 None:intergenic
GTGTTCCACTATTGCCCTAC+TGG 0.465761 1:+49314199 MsaT002920.1:CDS
TGAAGCTTTCCAAGGAGTTA+GGG 0.476348 1:+49312187 MsaT002920.1:CDS
CCAATTTGAGGTTGTGTCCA+AGG 0.478928 1:+49312302 MsaT002920.1:CDS
TGGAAGTGAAACAGCAAGAC+TGG 0.490760 1:+49312022 MsaT002920.1:CDS
TTCACTTAGACACCAATTTG+AGG 0.493024 1:+49312290 MsaT002920.1:CDS
ATACAAGTGTTCAAGTTGCT+TGG 0.493063 1:-49312265 None:intergenic
GGTACAGAAGCAGGCCAGTA+GGG 0.495028 1:-49314213 None:intergenic
AGGCTGTAGCCCTAACTCCT+TGG 0.496406 1:-49312196 None:intergenic
TGTTCAGGTTGTGAAGTTGA+AGG 0.499837 1:+49313969 MsaT002920.1:intron
GTGTGTTTGTTAAGGAATGA+TGG 0.508746 1:+49312002 MsaT002920.1:intron
GGTTCCAAAATAGGCGTGCT+AGG 0.516451 1:+49312235 MsaT002920.1:CDS
TACAACCAATGCTCATATCA+AGG 0.516637 1:+49311637 MsaT002920.1:CDS
TAGTTATAAGGTACAGAAGC+AGG 0.517499 1:-49314222 None:intergenic
CAACAGTTGAACATTGATGA+AGG 0.518077 1:+49314135 MsaT002920.1:CDS
AAAACAGCAGCTTCAGGATG+AGG 0.518698 1:+49312326 MsaT002920.1:CDS
AGCATTGGTTGTAGTTGTAG+AGG 0.525273 1:-49311627 None:intergenic
TAGATAATTATGGAATGGAA+TGG 0.526946 1:+49311559 None:intergenic
AACTCTTGTGGTAGAATGCA+AGG 0.527714 1:+49314012 MsaT002920.1:CDS
GAGTGAACAGATGGAATCAC+TGG 0.535577 1:+49312119 MsaT002920.1:CDS
AGGTACAGAAGCAGGCCAGT+AGG 0.540474 1:-49314214 None:intergenic
GCGATTAACGAGTGAACAGA+TGG 0.542500 1:+49312110 MsaT002920.1:CDS
ACAGATAGATAATTATGGAA+TGG 0.549629 1:+49311554 None:intergenic
CTTAAGGAAGACTCTGGTTG+AGG 0.556116 1:-49311601 None:intergenic
TTGCTCCAACAGAGAGGTGA+AGG 0.556685 1:+49314101 MsaT002920.1:CDS
AGATCAAACTAGACCCTCAA+AGG 0.558248 1:+49312160 MsaT002920.1:CDS
AACAGTAGAAAGCACATCAG+AGG 0.578567 1:+49314068 MsaT002920.1:CDS
AAAGAACAAGGTAACTCTTG+TGG 0.599344 1:+49314000 MsaT002920.1:CDS
AGGAAGCTTAAGGAAGACTC+TGG 0.609695 1:-49311607 None:intergenic
AAGGAAGATGAAGCTTTCCA+AGG 0.615641 1:+49312179 MsaT002920.1:CDS
AAGGCTATGTTGAAAGAACA+AGG 0.632753 1:+49313988 MsaT002920.1:CDS
TCCAAAATAGGCGTGCTAGG+TGG 0.637372 1:+49312238 MsaT002920.1:CDS
TTACTATACAGAAATGTCAG+TGG 0.650416 1:+49314035 MsaT002920.1:CDS
TGCTCCAACAGAGAGGTGAA+GGG 0.655501 1:+49314102 MsaT002920.1:CDS
AACCAAATAGCAATTTGACG+AGG 0.698239 1:-49312216 None:intergenic
TCTACGTTGCTCCAACAGAG+AGG 0.709736 1:+49314095 MsaT002920.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 49311568 49314254 49311568 ID=MsaG002920
Chr1 mRNA 49311568 49314254 49311568 ID=MsaT002920.1;Parent=MsaG002920
Chr1 exon 49311568 49311658 49311568 ID=MsaT002920.1.exon1;Parent=MsaT002920.1
Chr1 CDS 49311568 49311658 49311568 ID=cds.MsaT002920.1;Parent=MsaT002920.1
Chr1 exon 49312016 49312347 49312016 ID=MsaT002920.1.exon2;Parent=MsaT002920.1
Chr1 CDS 49312016 49312347 49312016 ID=cds.MsaT002920.1;Parent=MsaT002920.1
Chr1 exon 49313976 49314254 49313976 ID=MsaT002920.1.exon3;Parent=MsaT002920.1
Chr1 CDS 49313976 49314254 49313976 ID=cds.MsaT002920.1;Parent=MsaT002920.1
Gene Sequence

>MsaT002920.1

ATGGAATGGAATGGAAACACAAGAAGCTTTGTTCCTCAACCAGAGTCTTCCTTAAGCTTCCTCTACAACTACAACCAATGCTCATATCAAGGAATGATGGAAGTGAAACAGCAAGACTGGACAGATACAATGTTCCCAGAAATGGAAAAAATGATGAAGTATGGGAACTTGCAAGAAAAGAAAAAGCGATTAACGAGTGAACAGATGGAATCACTGGAGAGTAGTTTTCAAGAGGAGATCAAACTAGACCCTCAAAGGAAGATGAAGCTTTCCAAGGAGTTAGGGCTACAGCCTCGTCAAATTGCTATTTGGTTCCAAAATAGGCGTGCTAGGTGGAAGACCAAGCAACTTGAACACTTGTATGATTCACTTAGACACCAATTTGAGGTTGTGTCCAAGGAAAAACAGCAGCTTCAGGATGAGGTTGTGAAGTTGAAGGCTATGTTGAAAGAACAAGGTAACTCTTGTGGTAGAATGCAAGGTTACTATACAGAAATGTCAGTGGAAGAAACAGAAACAGTAGAAAGCACATCAGAGGCAGCTCTACGTTGCTCCAACAGAGAGGTGAAGGGAAAGATTCATCAACAGTTGAACATTGATGAAGGAAACTGTTGTTTCAATTTAGAGGAGTATAACATTAATAACAGTGTTCCACTATTGCCCTACTGGCCTGCTTCTGTACCTTATAACTATCACCCTTAA

Protein sequence

>MsaT002920.1

MEWNGNTRSFVPQPESSLSFLYNYNQCSYQGMMEVKQQDWTDTMFPEMEKMMKYGNLQEKKKRLTSEQMESLESSFQEEIKLDPQRKMKLSKELGLQPRQIAIWFQNRRARWKTKQLEHLYDSLRHQFEVVSKEKQQLQDEVVKLKAMLKEQGNSCGRMQGYYTEMSVEETETVESTSEAALRCSNREVKGKIHQQLNIDEGNCCFNLEEYNINNSVPLLPYWPASVPYNYHP*