AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG002922

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002922 MtrunA17_Chr1g0179611 98.352 546 7 1 1 544 1 546 0.0 1122
MsaG002922 MtrunA17_Chr4g0027671 67.170 530 166 2 12 533 7 536 0.0 758
MsaG002922 MtrunA17_Chr2g0320631 68.550 531 148 5 11 530 7 529 0.0 749
MsaG002922 MtrunA17_Chr5g0407121 53.509 456 154 4 23 477 17 415 8.25e-172 493
MsaG002922 MtrunA17_Chr8g0370321 52.203 454 157 4 23 476 17 410 9.01e-169 485
MsaG002922 MtrunA17_Chr3g0116701 38.608 158 90 4 29 186 80 230 3.95e-27 114
MsaG002922 MtrunA17_Chr3g0116701 30.899 178 88 6 317 476 259 419 2.14e-17 84.7
MsaG002922 MtrunA17_Chr3g0139371 36.250 160 95 4 27 186 58 210 7.81e-27 112
MsaG002922 MtrunA17_Chr3g0139371 32.955 176 91 3 317 478 238 400 2.59e-21 96.3
MsaG002922 MtrunA17_Chr4g0058241 37.580 157 91 4 29 185 84 233 1.57e-26 112
MsaG002922 MtrunA17_Chr4g0058241 30.857 175 86 6 317 473 263 420 7.05e-16 80.1
MsaG002922 MtrunA17_Chr3g0123491 32.941 170 104 5 19 185 66 228 4.72e-24 104
MsaG002922 MtrunA17_Chr3g0123491 33.654 104 63 1 317 420 260 357 1.98e-13 72.4
MsaG002922 MtrunA17_Chr8g0369241 40.136 147 82 3 41 186 136 277 4.27e-23 103
MsaG002922 MtrunA17_Chr8g0369241 38.333 120 64 3 308 423 273 386 9.62e-16 80.9
MsaG002922 MtrunA17_Chr6g0480541 29.775 178 109 5 306 476 1032 1200 2.50e-19 92.4
MsaG002922 MtrunA17_Chr6g0480541 33.125 160 96 4 30 185 886 1038 3.73e-15 79.0
MsaG002922 MtrunA17_Chr5g0437131 29.944 177 110 5 306 476 537 705 5.73e-18 87.4
MsaG002922 MtrunA17_Chr5g0437131 33.548 155 94 3 35 185 394 543 7.80e-15 77.8
MsaG002922 MtrunA17_Chr1g0210061 32.500 160 97 5 29 183 596 749 3.36e-17 85.5
MsaG002922 MtrunA17_Chr1g0210061 31.720 186 102 5 308 476 748 925 3.02e-15 79.3
MsaG002922 MtrunA17_Chr4g0065851 34.254 181 92 9 306 477 155 317 2.96e-12 69.3
MsaG002922 MtrunA17_Chr4g0045841 32.292 192 107 9 289 476 168 340 7.27e-12 67.4
MsaG002922 MtrunA17_Chr8g0348481 33.898 177 87 9 308 476 169 323 4.43e-11 64.7
MsaG002922 MtrunA17_Chr8g0387401 28.870 239 132 9 289 521 165 371 5.83e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG002922 AT3G53030.1 72.368 532 124 6 10 535 8 522 0.0 786
MsaG002922 AT3G44850.1 69.392 526 145 5 9 524 10 529 0.0 753
MsaG002922 AT5G22840.1 69.349 522 147 4 10 528 11 522 0.0 751
MsaG002922 AT4G35500.1 53.304 454 158 4 25 478 19 418 6.09e-172 493
MsaG002922 AT2G17530.1 51.101 454 167 3 25 478 19 417 1.99e-163 472
MsaG002922 AT2G17530.3 51.101 454 167 3 25 478 19 417 1.99e-163 472
MsaG002922 AT4G35500.2 51.429 455 166 5 25 478 19 419 9.03e-163 470
MsaG002922 AT2G17530.2 49.868 379 135 3 25 403 19 342 2.47e-124 369
MsaG002922 AT4G24740.4 37.209 172 100 5 29 200 84 247 2.93e-28 115
MsaG002922 AT4G24740.3 37.209 172 100 5 29 200 84 247 4.70e-28 115
MsaG002922 AT4G24740.3 35.833 120 70 2 284 403 231 343 2.02e-14 75.1
MsaG002922 AT4G24740.7 37.209 172 100 5 29 200 46 209 7.94e-28 115
MsaG002922 AT4G24740.7 29.858 211 112 7 284 476 193 385 8.63e-18 85.9
MsaG002922 AT4G24740.1 37.209 172 100 5 29 200 84 247 1.13e-27 115
MsaG002922 AT4G24740.1 29.858 211 112 7 284 476 231 423 9.37e-18 86.3
MsaG002922 AT4G32660.2 35.625 160 96 3 27 186 55 207 2.43e-26 110
MsaG002922 AT4G32660.2 42.574 101 51 3 317 416 235 329 2.49e-15 78.2
MsaG002922 AT4G32660.3 35.625 160 96 3 27 186 47 199 3.33e-26 110
MsaG002922 AT4G32660.3 34.706 170 93 5 317 476 227 388 8.30e-18 85.9
MsaG002922 AT4G32660.1 35.625 160 96 3 27 186 55 207 3.67e-26 110
MsaG002922 AT4G32660.1 34.706 170 93 5 317 476 235 396 9.34e-18 85.9
MsaG002922 AT3G53570.1 36.471 170 98 5 20 186 89 251 6.92e-26 111
MsaG002922 AT3G53570.1 31.844 179 88 5 316 476 281 443 1.42e-17 85.9
MsaG002922 AT3G53570.4 36.471 170 98 5 20 186 89 251 6.92e-26 111
MsaG002922 AT3G53570.4 31.844 179 88 5 316 476 281 443 1.42e-17 85.9
MsaG002922 AT3G53570.2 36.471 170 98 5 20 186 89 251 6.92e-26 111
MsaG002922 AT3G53570.2 31.844 179 88 5 316 476 281 443 1.42e-17 85.9
MsaG002922 AT3G53570.3 36.471 170 98 5 20 186 75 237 7.44e-26 110
MsaG002922 AT3G53570.3 31.844 179 88 5 316 476 267 429 1.67e-17 85.5
MsaG002922 AT3G53570.5 36.471 170 98 5 20 186 75 237 7.44e-26 110
MsaG002922 AT3G53570.5 31.844 179 88 5 316 476 267 429 1.67e-17 85.5
MsaG002922 AT4G24740.5 37.748 151 87 4 50 200 1 144 1.32e-24 103
MsaG002922 AT4G24740.5 35.833 120 70 2 284 403 128 240 1.23e-14 74.3
MsaG002922 AT4G24740.6 37.748 151 87 4 50 200 1 144 3.18e-24 103
MsaG002922 AT4G24740.6 29.858 211 112 7 284 476 128 320 6.65e-18 85.5
MsaG002922 AT4G24740.9 37.748 151 87 4 50 200 1 144 3.18e-24 103
MsaG002922 AT4G24740.9 29.858 211 112 7 284 476 128 320 6.65e-18 85.5
MsaG002922 AT4G24740.2 37.748 151 87 4 50 200 1 144 3.18e-24 103
MsaG002922 AT4G24740.2 29.858 211 112 7 284 476 128 320 6.65e-18 85.5
MsaG002922 AT5G35980.1 40.000 150 84 3 43 191 121 265 4.74e-24 107
MsaG002922 AT5G35980.1 38.525 122 65 4 306 423 253 368 3.09e-15 79.7
MsaG002922 AT5G35980.3 38.415 164 91 4 33 191 72 230 4.78e-24 107
MsaG002922 AT5G35980.3 38.525 122 65 4 306 423 218 333 4.08e-15 79.3
MsaG002922 AT5G35980.2 38.415 164 91 4 33 191 107 265 7.70e-24 106
MsaG002922 AT5G35980.2 38.525 122 65 4 306 423 253 368 4.08e-15 79.0
MsaG002922 AT1G73460.2 30.726 179 108 5 306 477 992 1161 6.94e-21 97.8
MsaG002922 AT1G73460.2 32.886 149 91 3 41 185 855 998 3.29e-13 73.2
MsaG002922 AT1G73460.1 30.726 179 108 5 306 477 992 1161 6.94e-21 97.8
MsaG002922 AT1G73460.1 32.886 149 91 3 41 185 855 998 3.29e-13 73.2
MsaG002922 AT1G73450.1 30.726 179 108 5 306 477 975 1144 8.67e-21 97.4
MsaG002922 AT1G73450.1 32.886 149 91 3 41 185 838 981 3.69e-13 73.2
MsaG002922 AT4G24740.10 29.858 211 112 7 284 476 25 217 4.36e-19 86.7
MsaG002922 AT4G24740.8 29.858 211 112 7 284 476 25 217 4.36e-19 86.7
MsaG002922 AT2G40120.1 31.073 177 110 5 306 477 395 564 9.94e-19 90.1
MsaG002922 AT2G40120.1 33.548 155 94 3 35 185 252 401 1.47e-14 77.0
MsaG002922 AT4G32660.4 34.706 170 93 5 317 476 123 284 4.74e-18 85.1
MsaG002922 AT4G32660.4 36.364 99 57 2 88 186 3 95 7.47e-14 72.8
MsaG002922 AT3G17750.1 29.545 176 112 4 306 476 961 1129 7.15e-18 88.2
MsaG002922 AT3G17750.1 32.500 160 97 4 30 185 815 967 5.97e-14 75.5
MsaG002922 AT3G25840.1 31.250 160 99 5 29 183 603 756 3.11e-15 79.7
MsaG002922 AT3G25840.1 32.571 175 97 5 317 476 764 932 4.26e-14 75.9
MsaG002922 AT3G53640.1 33.146 178 93 7 317 476 470 639 3.82e-15 79.0
MsaG002922 AT3G53640.1 30.625 160 100 5 29 183 309 462 5.18e-13 72.0
MsaG002922 AT1G13350.1 30.625 160 100 5 29 183 428 581 6.28e-15 78.6
MsaG002922 AT1G13350.1 30.682 176 100 5 317 476 589 758 1.85e-14 77.0
MsaG002922 AT3G25840.2 31.250 160 99 5 29 183 341 494 7.40e-15 78.2
MsaG002922 AT3G25840.2 32.571 175 97 5 317 476 502 670 4.36e-14 75.5
MsaG002922 AT1G13350.2 30.625 160 100 5 29 183 455 608 8.86e-15 78.2
MsaG002922 AT1G13350.2 30.682 176 100 5 317 476 616 785 2.68e-14 76.6
MsaG002922 AT1G13350.3 30.625 160 100 5 29 183 501 654 1.57e-14 77.4
MsaG002922 AT1G13350.3 30.682 176 100 5 317 476 662 831 3.85e-14 75.9
MsaG002922 AT1G73670.1 30.526 190 106 9 306 486 220 392 3.32e-12 69.7
MsaG002922 AT3G59790.1 29.333 225 126 9 289 510 173 367 7.42e-12 67.8
MsaG002922 AT2G30980.1 31.148 183 101 8 315 494 211 371 4.97e-11 65.5

Find 138 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCATAAGCACTTTATTTA+AGG 0.062791 1:-49326609 None:intergenic
AGAGATATGCTTTCATATTT+TGG 0.133189 1:+49322686 MsaT002922.1:CDS
CATCTTTGTCCTTGTTCTTC+TGG 0.137125 1:-49326826 None:intergenic
CGGGATTTCTTCAATAGATA+TGG 0.256557 1:+49326551 MsaT002922.1:CDS
GCCCTTAAATAAAGTGCTTA+TGG 0.273324 1:+49326607 MsaT002922.1:CDS
GAATGTAGGTCCCCGTCTTT+TGG 0.274592 1:+49326748 MsaT002922.1:CDS
GCATTAATGATGGAACTTCT+TGG 0.282888 1:+49326407 MsaT002922.1:CDS
TATACTTAATAAGAGTTAAT+AGG 0.290620 1:-49322629 None:intergenic
TCTCCACTGTTTGGCTCGCT+TGG 0.295410 1:+49321391 MsaT002922.1:CDS
CCTTGGAATACTTTCTTTGA+TGG 0.309821 1:-49326920 None:intergenic
TTAGACCATTTCAAGCATTC+TGG 0.313545 1:+49322558 MsaT002922.1:CDS
TCCATAAGCACTTTATTTAA+GGG 0.317347 1:-49326608 None:intergenic
ATTCTACATCTGCGGATCTT+TGG 0.322906 1:+49323282 MsaT002922.1:CDS
TCACGGATTGCCTATCAATA+TGG 0.323581 1:+49322659 MsaT002922.1:CDS
TTGACTTTGGTAATGCTTGC+TGG 0.327850 1:+49323186 MsaT002922.1:CDS
ATAGTCTTGTCTTTACTGTT+AGG 0.332521 1:-49322837 None:intergenic
CGTTCAGAGCAAGCTTGGTT+GGG 0.339812 1:+49321362 MsaT002922.1:CDS
GGGGTCATTTCTCCACTGTT+TGG 0.341167 1:+49321382 MsaT002922.1:CDS
ATATCCGTCGGCTGCGATTC+TGG 0.344849 1:+49326585 MsaT002922.1:CDS
CTCCACTGTTTGGCTCGCTT+GGG 0.347766 1:+49321392 MsaT002922.1:CDS
ATCTTCTGTGCCTTCGTCTT+CGG 0.358366 1:-49321277 None:intergenic
AAGAGGAGCACCAGATTTCC+TGG 0.363522 1:-49322808 None:intergenic
GAGGCCATGGAGGCTGGAAT+TGG 0.365219 1:+49326854 MsaT002922.1:CDS
ATCAACAATTGATCCGTCTA+AGG 0.368658 1:+49322782 MsaT002922.1:CDS
ATACAAACATGCTGTCCATT+TGG 0.376683 1:-49322582 None:intergenic
ATAGTGGTGACAACTTTGAT+AGG 0.377741 1:+49323360 MsaT002922.1:CDS
ATCTCTTTAACCATATTGAT+AGG 0.403343 1:-49322669 None:intergenic
GAGAGGGAGGCCATGGAGGC+TGG 0.404591 1:+49326848 MsaT002922.1:CDS
TGATTAGAAGACGGCATTGA+TGG 0.409832 1:-49326773 None:intergenic
TCTAAGGATCCCAGGAAATC+TGG 0.413304 1:+49322798 MsaT002922.1:CDS
GGAGGTGAAATCGCTGCTTT+CGG 0.420256 1:-49321256 None:intergenic
AAATGGACAGCATGTTTGTA+TGG 0.420622 1:+49322584 MsaT002922.1:CDS
TTCAAGCATTCTGGTCCAAA+TGG 0.428400 1:+49322567 MsaT002922.1:CDS
AGAGACACTAAAACATCAAA+TGG 0.428919 1:+49322879 MsaT002922.1:CDS
CTAGTTCTGCTGGCACTAGT+AGG 0.430061 1:+49323057 MsaT002922.1:CDS
ACATCCATGAGCTGCTTGCT+TGG 0.434213 1:-49322943 None:intergenic
TGAACTACTTGCGGCTGCCT+TGG 0.440976 1:-49326937 None:intergenic
TTGGACCAGAATGCTTGAAA+TGG 0.452574 1:-49322563 None:intergenic
CGGTGCCCAGAGGTTATCCT+TGG 0.454662 1:+49323251 MsaT002922.1:CDS
GGCTACCACGCCGTTCGAAT+CGG 0.458904 1:+49321312 MsaT002922.1:CDS
TCTCTTGCAGAACTTGCATT+CGG 0.467917 1:-49323026 None:intergenic
GCATTACACAGAGGCTGCCC+TGG 0.469898 1:+49322467 MsaT002922.1:CDS
CCGGCTGTAGGTCGTTTCTC+AGG 0.474020 1:-49326701 None:intergenic
GAAGTAGCAGTTTCAGCACC+AGG 0.474460 1:-49326800 None:intergenic
CATATCTATTGAAGAAATCC+CGG 0.482137 1:-49326550 None:intergenic
CAAAGATGAGAGGGAGGCCA+TGG 0.484725 1:+49326841 MsaT002922.1:CDS
CATTGATGGCTCCAAAAGAC+GGG 0.487038 1:-49326759 None:intergenic
TCGTTCAGAGCAAGCTTGGT+TGG 0.487627 1:+49321361 MsaT002922.1:CDS
AGCTCATGGATGTGCGGAGA+AGG 0.490342 1:+49322953 MsaT002922.1:CDS
GAAATCGCTGCTTTCGGTGG+TGG 0.490996 1:-49321250 None:intergenic
GCAAAATCGTTATCTCATCC+AGG 0.491981 1:-49322485 None:intergenic
AAGTGCAAGTTAGTTGACTT+TGG 0.494805 1:+49323173 MsaT002922.1:CDS
TTTGATCCAAGGATAACCTC+TGG 0.500060 1:-49323257 None:intergenic
CGGATGTAGCAGGCATTGAC+CGG 0.500642 1:-49326720 None:intergenic
TGGAATGAAGTCAAAAGAGC+AGG 0.504504 1:+49323094 MsaT002922.1:CDS
AAAGCCAAGCAAGCAGCTCA+TGG 0.505034 1:+49322939 MsaT002922.1:CDS
GTTCAGAGCAAGCTTGGTTG+GGG 0.505953 1:+49321363 MsaT002922.1:CDS
CAGTAAAGACAAGACTATGC+TGG 0.508183 1:+49322842 MsaT002922.1:CDS
AAGAAGAACGAACAGAATGA+TGG 0.508503 1:-49321419 None:intergenic
TATAATTCTCAGATCGCACT+AGG 0.509453 1:+49326515 MsaT002922.1:intron
GAACAAGCTTCTAGTTCTGC+TGG 0.516084 1:+49323047 MsaT002922.1:CDS
CATTCATCCACGGATGTAGC+AGG 0.517627 1:-49326730 None:intergenic
TATGTCGTTCAGAGCAAGCT+TGG 0.518360 1:+49321357 MsaT002922.1:CDS
GTTTCCAATTCCAGCCTCCA+TGG 0.523041 1:-49326858 None:intergenic
TTACTGTTAGGAAGAATAAG+AGG 0.526154 1:-49322825 None:intergenic
TTCTGTGCCTTCGTCTTCGG+AGG 0.532445 1:-49321274 None:intergenic
GACATAACGACCGGAGCTGA+AGG 0.539436 1:-49321340 None:intergenic
AAAGAGCAGGGCAACAGAAG+AGG 0.541507 1:+49323107 MsaT002922.1:CDS
ATCGGCGATACCTTCAGCTC+CGG 0.545934 1:+49321330 MsaT002922.1:CDS
GTCAATGCCTGCTACATCCG+TGG 0.547156 1:+49326723 MsaT002922.1:CDS
GAACAAGGACAAAGATGAGA+GGG 0.551701 1:+49326832 MsaT002922.1:CDS
GAGAAGGAGGCATCTGAAGA+TGG 0.552642 1:+49322969 MsaT002922.1:CDS
CTACATCCGTGGATGAATGT+AGG 0.555309 1:+49326734 MsaT002922.1:CDS
GCATTGATGGCTCCAAAAGA+CGG 0.555544 1:-49326760 None:intergenic
GGACCACTTAGCATTAATGA+TGG 0.555688 1:+49326397 MsaT002922.1:intron
GATTTCCTGGGATCCTTAGA+CGG 0.556902 1:-49322795 None:intergenic
GATCAATTGTTGATAAGAGA+AGG 0.558128 1:-49322773 None:intergenic
TCAAAGTTGTCACCACTATG+AGG 0.558782 1:-49323356 None:intergenic
GTTCCATCATTAATGCTAAG+TGG 0.559588 1:-49326400 None:intergenic
ATATCTATTGAAGAAATCCC+GGG 0.566218 1:-49326549 None:intergenic
TTGATCCGTCTAAGGATCCC+AGG 0.566867 1:+49322790 MsaT002922.1:CDS
GTATTATTTGATCCTCATAG+TGG 0.567179 1:+49323344 MsaT002922.1:CDS
ATTAAGTATAGTGATTATCA+CGG 0.569731 1:+49322642 MsaT002922.1:CDS
AAGAAATTTATTACCTTCCG+CGG 0.571931 1:-49326437 None:intergenic
CACTAGTAGGTTATCAGATG+CGG 0.576983 1:+49323070 MsaT002922.1:CDS
CAAGGACAAAGATGAGAGGG+AGG 0.580802 1:+49326835 MsaT002922.1:CDS
TGGGTCAAACAAATGGGTGG+CGG 0.584188 1:+49321216 None:intergenic
TCTTGGAATGATGCCGCGGA+AGG 0.584206 1:+49326424 MsaT002922.1:CDS
CTATCAAGATGAACTACTTG+CGG 0.587404 1:-49326946 None:intergenic
TTGATCCAAGGATAACCTCT+GGG 0.587839 1:-49323256 None:intergenic
AGAAGAACGAACAGAATGAT+GGG 0.588817 1:-49321418 None:intergenic
GAAGGTATCGCCGATTCGAA+CGG 0.589832 1:-49321322 None:intergenic
GGTGAAATCGCTGCTTTCGG+TGG 0.592150 1:-49321253 None:intergenic
TCATGGATGTGCGGAGAAGG+AGG 0.595831 1:+49322956 MsaT002922.1:CDS
CCTGAGAAACGACCTACAGC+CGG 0.605154 1:+49326701 MsaT002922.1:CDS
GATGGTAATCATGAAACCTC+TGG 0.606715 1:+49322987 MsaT002922.1:CDS
ATAATTCTCAGATCGCACTA+GGG 0.611148 1:+49326516 MsaT002922.1:intron
AGAGGAGCACCAGATTTCCT+GGG 0.614355 1:-49322807 None:intergenic
AGATGATTCTACAGCACCAG+AGG 0.614399 1:-49323003 None:intergenic
TAGTGGTGACAACTTTGATA+GGG 0.616977 1:+49323361 MsaT002922.1:CDS
AAGTAGCAGTTTCAGCACCA+GGG 0.617453 1:-49326799 None:intergenic
ATCCCAAGCGAGCCAAACAG+TGG 0.619472 1:-49321394 None:intergenic
TTTGATGGAGAATGCATGAG+TGG 0.621786 1:-49326905 None:intergenic
CAAAATCGTTATCTCATCCA+GGG 0.625815 1:-49322484 None:intergenic
CCGTCTTCTAATCACAACCC+TGG 0.626806 1:+49326782 MsaT002922.1:CDS
CCATCAAAGAAAGTATTCCA+AGG 0.627091 1:+49326920 MsaT002922.1:CDS
ATATTCAGACAAGACAGTAT+CGG 0.630386 1:+49323231 MsaT002922.1:CDS
TACTTCTGACCAGAAGAACA+AGG 0.631653 1:+49326817 MsaT002922.1:CDS
AGGGCCAGAATCGCAGCCGA+CGG 0.635004 1:-49326589 None:intergenic
AGAACAAGGACAAAGATGAG+AGG 0.640119 1:+49326831 MsaT002922.1:CDS
GCTCTGAACGACATAACGAC+CGG 0.640589 1:-49321349 None:intergenic
ATCAAAATATTCTACATCTG+CGG 0.641197 1:+49323274 MsaT002922.1:CDS
AGATGAGAGGGAGGCCATGG+AGG 0.643015 1:+49326844 MsaT002922.1:CDS
AACTTCTTGGAATGATGCCG+CGG 0.644175 1:+49326420 MsaT002922.1:CDS
CCAGGGTTGTGATTAGAAGA+CGG 0.645495 1:-49326782 None:intergenic
AGTAGGTTATCAGATGCGGA+TGG 0.646147 1:+49323074 MsaT002922.1:CDS
CGGGGACCTACATTCATCCA+CGG 0.647503 1:-49326740 None:intergenic
GTGATTTGAGACATATCCGT+CGG 0.649441 1:+49326573 MsaT002922.1:CDS
GTTTCTCAGGAACAAAGTCA+AGG 0.651456 1:-49326688 None:intergenic
TAATTCTCAGATCGCACTAG+GGG 0.655674 1:+49326517 MsaT002922.1:intron
GGAATGAAGTCAAAAGAGCA+GGG 0.655804 1:+49323095 MsaT002922.1:CDS
GCAAGCAGCTCATGGATGTG+CGG 0.656226 1:+49322947 MsaT002922.1:CDS
ACAGAAGATTACCGTCGCGG+TGG 0.665098 1:+49321291 MsaT002922.1:CDS
GAGTTAATAGGTTATCCCCA+AGG 0.667992 1:-49322617 None:intergenic
GCAGTTTCTGTCTCACTGTG+CGG 0.668245 1:-49323133 None:intergenic
ATTCTCAGATCGCACTAGGG+GGG 0.670913 1:+49326519 MsaT002922.1:intron
AGCAGGCATTGACCGGCTGT+AGG 0.674560 1:-49326713 None:intergenic
AATTCTCAGATCGCACTAGG+GGG 0.677507 1:+49326518 MsaT002922.1:intron
GATTTCACCTCCGAAGACGA+AGG 0.680908 1:+49321267 MsaT002922.1:CDS
CGGCGTGGTAGCCACCGCGA+CGG 0.681696 1:-49321302 None:intergenic
TGGAGATTTGCAGAGATACG+TGG 0.683349 1:+49322419 MsaT002922.1:intron
CTTGCACTTGACATCAGCTG+AGG 0.686469 1:-49323159 None:intergenic
TGACAACTTTGATAGGGACG+AGG 0.691000 1:+49323367 MsaT002922.1:CDS
GGCACAGAAGATTACCGTCG+CGG 0.695054 1:+49321288 MsaT002922.1:CDS
GAGTGCTCAGCATTACACAG+AGG 0.702864 1:+49322458 MsaT002922.1:CDS
TATCGCCGATTCGAACGGCG+TGG 0.712115 1:-49321317 None:intergenic
ATTGATGGCTCCAAAAGACG+GGG 0.727950 1:-49326758 None:intergenic
AAGACAGTATCGGTGCCCAG+AGG 0.753096 1:+49323241 MsaT002922.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 49321228 49326955 49321228 ID=MsaG002922
Chr1 mRNA 49321228 49326955 49321228 ID=MsaT002922.1;Parent=MsaG002922
Chr1 exon 49321228 49321428 49321228 ID=MsaT002922.1.exon1;Parent=MsaT002922.1
Chr1 CDS 49321228 49321428 49321228 ID=cds.MsaT002922.1;Parent=MsaT002922.1
Chr1 exon 49322432 49323388 49322432 ID=MsaT002922.1.exon2;Parent=MsaT002922.1
Chr1 CDS 49322432 49323388 49322432 ID=cds.MsaT002922.1;Parent=MsaT002922.1
Chr1 exon 49326398 49326445 49326398 ID=MsaT002922.1.exon3;Parent=MsaT002922.1
Chr1 CDS 49326398 49326445 49326398 ID=cds.MsaT002922.1;Parent=MsaT002922.1
Chr1 exon 49326527 49326955 49326527 ID=MsaT002922.1.exon4;Parent=MsaT002922.1
Chr1 CDS 49326527 49326955 49326527 ID=cds.MsaT002922.1;Parent=MsaT002922.1
Gene Sequence

>MsaT002922.1

ATGGGTGGCGGACACGACGATACCACCACCGAAAGCAGCGATTTCACCTCCGAAGACGAAGGCACAGAAGATTACCGTCGCGGTGGCTACCACGCCGTTCGAATCGGCGATACCTTCAGCTCCGGTCGTTATGTCGTTCAGAGCAAGCTTGGTTGGGGTCATTTCTCCACTGTTTGGCTCGCTTGGGATTCCCATCATTCTAGATACGTGGCTTTGAAAGTTCAGAAGAGTGCTCAGCATTACACAGAGGCTGCCCTGGATGAGATAACGATTTTGCAACAGATTGCGGAGGGAGACACTGATGATAAAAAATGTGTGGTCAAGCTTTTAGACCATTTCAAGCATTCTGGTCCAAATGGACAGCATGTTTGTATGGTTTTTGAGTACCTTGGGGATAACCTATTAACTCTTATTAAGTATAGTGATTATCACGGATTGCCTATCAATATGGTTAAAGAGATATGCTTTCATATTTTGGTTGGATTGGATTACTTACATAAGCAGCTTTCTATCATACATACTGACTTAAAACCCGAAAATATCCTTCTCTTATCAACAATTGATCCGTCTAAGGATCCCAGGAAATCTGGTGCTCCTCTTATTCTTCCTAACAGTAAAGACAAGACTATGCTGGAGTCAACGACTGCAAGAGACACTAAAACATCAAATGGAGATTTTATTAAGAATCATAAGAAAAATATTAAGAGAAAAGCCAAGCAAGCAGCTCATGGATGTGCGGAGAAGGAGGCATCTGAAGATGGTAATCATGAAACCTCTGGTGCTGTAGAATCATCTCCGAATGCAAGTTCTGCAAGAGAACAAGCTTCTAGTTCTGCTGGCACTAGTAGGTTATCAGATGCGGATGGAATGAAGTCAAAAGAGCAGGGCAACAGAAGAGGAAGCCGCACAGTGAGACAGAAACTGCTTGCCTCAGCTGATGTCAAGTGCAAGTTAGTTGACTTTGGTAATGCTTGCTGGACATATAAACAGTTTACAAATGATATTCAGACAAGACAGTATCGGTGCCCAGAGGTTATCCTTGGATCAAAATATTCTACATCTGCGGATCTTTGGTCATTTGCTTGCATATGTTTTGAGCTTGCAACTGGAGATGTATTATTTGATCCTCATAGTGGTGACAACTTTGATAGGGACGAGGACCACTTAGCATTAATGATGGAACTTCTTGGAATGATGCCGCGGAAGATCGCACTAGGGGGGCGTTATTCCCGGGATTTCTTCAATAGATATGGTGATTTGAGACATATCCGTCGGCTGCGATTCTGGCCCTTAAATAAAGTGCTTATGGAGAAGTACGATTTTAGTGAGAAAGATGCAAGTGACATGAGTGACTTTTTGGTTTCTATCCTTGACTTTGTTCCTGAGAAACGACCTACAGCCGGTCAATGCCTGCTACATCCGTGGATGAATGTAGGTCCCCGTCTTTTGGAGCCATCAATGCCGTCTTCTAATCACAACCCTGGTGCTGAAACTGCTACTTCTGACCAGAAGAACAAGGACAAAGATGAGAGGGAGGCCATGGAGGCTGGAATTGGAAACATTGCTATCAATTCAGATTCTAAACCACTCATGCATTCTCCATCAAAGAAAGTATTCCAAGGCAGCCGCAAGTAG
Protein sequence

>MsaT002922.1

MGGGHDDTTTESSDFTSEDEGTEDYRRGGYHAVRIGDTFSSGRYVVQSKLGWGHFSTVWLAWDSHHSRYVALKVQKSAQHYTEAALDEITILQQIAEGDTDDKKCVVKLLDHFKHSGPNGQHVCMVFEYLGDNLLTLIKYSDYHGLPINMVKEICFHILVGLDYLHKQLSIIHTDLKPENILLLSTIDPSKDPRKSGAPLILPNSKDKTMLESTTARDTKTSNGDFIKNHKKNIKRKAKQAAHGCAEKEASEDGNHETSGAVESSPNASSAREQASSSAGTSRLSDADGMKSKEQGNRRGSRTVRQKLLASADVKCKLVDFGNACWTYKQFTNDIQTRQYRCPEVILGSKYSTSADLWSFACICFELATGDVLFDPHSGDNFDRDEDHLALMMELLGMMPRKIALGGRYSRDFFNRYGDLRHIRRLRFWPLNKVLMEKYDFSEKDASDMSDFLVSILDFVPEKRPTAGQCLLHPWMNVGPRLLEPSMPSSNHNPGAETATSDQKNKDKDEREAMEAGIGNIAINSDSKPLMHSPSKKVFQGSRK*