AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003023

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003023 MtrunA17_Chr1g0181811 96.087 690 26 1 12 701 22 710 0.0 1375
MsaG003023 MtrunA17_Chr4g0030271 42.136 693 360 12 46 705 63 747 3.43e-134 416
MsaG003023 MtrunA17_Chr3g0127941 31.424 681 361 22 63 705 44 656 7.64e-59 211
MsaG003023 MtrunA17_Chr7g0276361 58.140 129 54 0 68 196 7 135 3.98e-45 171
MsaG003023 MtrunA17_Chr3g0091641 42.188 128 74 0 69 196 18 145 1.16e-25 112
MsaG003023 MtrunA17_Chr4g0061021 41.406 128 75 0 69 196 27 154 1.35e-25 112
MsaG003023 MtrunA17_Chr2g0304841 38.525 122 72 2 75 196 22 140 1.39e-22 103
MsaG003023 MtrunA17_Chr1g0161431 35.366 164 91 3 75 237 15 164 4.73e-22 101
MsaG003023 MtrunA17_Chr3g0134481 38.400 125 74 2 75 199 12 133 8.41e-21 90.9
MsaG003023 MtrunA17_Chr3g0137101 36.885 122 74 2 75 196 12 130 2.67e-20 95.9
MsaG003023 MtrunA17_Chr4g0074411 37.705 122 73 1 75 196 22 140 2.85e-20 95.5
MsaG003023 MtrunA17_Chr4g0067981 32.484 157 103 1 75 231 35 188 3.41e-20 95.5
MsaG003023 MtrunA17_Chr2g0294911 38.525 122 72 1 75 196 33 151 3.96e-20 95.1
MsaG003023 MtrunA17_Chr1g0149841 34.586 133 84 2 75 207 9 138 3.88e-19 91.3
MsaG003023 MtrunA17_Chr4g0074401 36.066 122 75 1 75 196 21 139 7.36e-19 90.9
MsaG003023 MtrunA17_Chr7g0275401 34.848 132 83 2 75 206 9 137 2.96e-18 86.3
MsaG003023 MtrunA17_Chr3g0123131 36.066 122 75 1 75 196 17 135 3.69e-18 88.6
MsaG003023 MtrunA17_Chr1g0149851 35.246 122 76 2 75 196 9 127 2.52e-17 85.9
MsaG003023 MtrunA17_Chr8g0376881 35.246 122 76 1 75 196 29 147 1.16e-16 84.0
MsaG003023 MtrunA17_Chr7g0275891 32.331 133 86 3 75 206 9 138 9.66e-15 76.3
MsaG003023 MtrunA17_Chr7g0256551 30.935 139 78 2 75 195 9 147 1.22e-14 74.3
MsaG003023 MtrunA17_Chr8g0383961 32.520 123 78 2 75 196 18 136 1.78e-13 71.2
MsaG003023 MtrunA17_Chr3g0128691 35.200 125 68 2 77 189 20 143 3.56e-13 67.8
MsaG003023 MtrunA17_Chr5g0414931 27.717 184 104 4 73 242 14 182 5.97e-13 69.3
MsaG003023 MtrunA17_Chr4g0074421 40.789 76 42 1 121 196 2 74 1.04e-12 67.8
MsaG003023 MtrunA17_Chr3g0134461 30.769 143 95 2 75 216 12 151 2.54e-12 66.6
MsaG003023 MtrunA17_Chr3g0082861 29.197 137 79 1 77 195 6 142 2.85e-12 65.5
MsaG003023 MtrunA17_Chr8g0352871 30.147 136 78 2 77 195 11 146 1.86e-11 63.5
MsaG003023 MtrunA17_Chr4g0059571 31.538 130 80 3 75 195 26 155 1.87e-11 64.3
MsaG003023 MtrunA17_Chr1g0170741 31.496 127 75 2 75 189 8 134 3.56e-11 63.2
MsaG003023 MtrunA17_Chr3g0118211 28.477 151 93 4 72 213 20 164 6.38e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003023 AT5G02810.1 47.724 725 269 22 26 705 36 695 2.42e-155 468
MsaG003023 AT5G60100.5 46.804 438 162 13 63 485 52 433 4.16e-91 294
MsaG003023 AT5G60100.3 46.804 438 162 13 63 485 52 433 4.16e-91 294
MsaG003023 AT5G60100.1 46.804 438 162 13 63 485 52 433 4.16e-91 294
MsaG003023 AT5G02810.2 44.667 600 229 21 151 705 10 551 1.82e-90 295
MsaG003023 AT5G60100.7 48.462 390 137 11 63 438 52 391 2.53e-89 286
MsaG003023 AT5G60100.6 48.462 390 137 11 63 438 52 391 2.53e-89 286
MsaG003023 AT5G60100.4 48.462 390 137 11 63 438 52 391 2.53e-89 286
MsaG003023 AT5G60100.2 44.086 465 162 14 63 485 52 460 2.07e-85 280
MsaG003023 AT2G46790.3 56.716 134 58 0 63 196 25 158 4.58e-50 182
MsaG003023 AT2G46790.1 56.716 134 58 0 63 196 25 158 5.72e-50 182
MsaG003023 AT5G24470.1 58.955 134 55 0 63 196 38 171 8.05e-49 181
MsaG003023 AT5G61380.1 40.625 128 76 0 69 196 13 140 1.39e-23 106
MsaG003023 AT3G16857.1 34.659 176 104 3 75 244 37 207 2.04e-21 99.8
MsaG003023 AT3G16857.2 34.659 176 104 3 75 244 37 207 2.10e-21 99.8
MsaG003023 AT2G25180.1 33.149 181 88 5 75 251 17 168 8.33e-21 97.8
MsaG003023 AT4G31920.1 38.017 121 72 2 75 195 17 134 3.04e-19 92.4
MsaG003023 AT2G01760.1 37.705 122 74 1 75 196 11 130 5.54e-18 87.0
MsaG003023 AT1G67710.1 35.252 139 83 2 75 209 11 146 1.54e-17 87.0
MsaG003023 AT3G16857.3 35.461 141 80 3 110 244 2 137 1.10e-15 81.6
MsaG003023 AT2G46790.2 70.732 41 12 0 156 196 1 41 5.08e-15 77.8
MsaG003023 AT3G62670.1 34.524 168 101 6 67 232 34 194 1.81e-14 76.6
MsaG003023 AT2G01760.2 37.168 113 68 2 84 196 49 158 2.43e-14 76.3
MsaG003023 AT1G10470.1 31.034 145 85 2 70 202 29 170 4.84e-14 73.2
MsaG003023 AT5G58080.1 31.967 122 79 1 75 196 18 135 1.05e-13 75.1
MsaG003023 AT1G59940.2 33.588 131 72 2 77 195 127 254 5.79e-13 71.2
MsaG003023 AT5G62920.1 35.772 123 64 4 67 189 41 148 6.50e-13 68.6
MsaG003023 AT1G19050.1 32.576 132 76 2 75 195 24 153 9.08e-13 68.6
MsaG003023 AT3G56380.1 33.077 130 77 2 75 194 20 149 1.92e-12 66.2
MsaG003023 AT1G10470.3 31.250 144 88 2 70 203 15 157 2.47e-12 68.2
MsaG003023 AT3G48100.1 35.772 123 65 4 67 189 41 149 4.02e-12 65.9
MsaG003023 AT1G59940.1 41.333 75 44 0 121 195 88 162 4.06e-12 67.0
MsaG003023 AT3G57040.2 28.986 138 79 2 77 195 11 148 1.19e-11 65.9
MsaG003023 AT3G57040.1 28.986 138 79 2 77 195 11 148 1.19e-11 65.9
MsaG003023 AT1G74890.1 32.353 136 77 3 67 202 34 154 7.94e-11 62.8

Find 174 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGATTTAATAGATTTC+TGG 0.104631 1:+50666718 None:intergenic
TGCACTTCTATACAAGATTA+TGG 0.158802 1:-50667359 MsaT003023.1:CDS
AGAAGTTGGGTCTTCTATTT+AGG 0.167561 1:+50663688 None:intergenic
CCAGATTGATTCTCACAATT+AGG 0.182320 1:+50663795 None:intergenic
ATTGCAAGCTTGGAAGATTT+TGG 0.231070 1:-50667446 MsaT003023.1:CDS
GATGGTTCCTCCAGTCTTAA+AGG 0.244142 1:-50664864 MsaT003023.1:CDS
TGCTAGCATCATTTGAGTTC+TGG 0.274365 1:+50663284 None:intergenic
AATGGAAGCAATGGACAAAA+TGG 0.284536 1:-50662839 MsaT003023.1:CDS
CTCTCTTATAACTGCAGATT+TGG 0.286096 1:+50663222 None:intergenic
TGCTAGCAACAACATTGATA+TGG 0.296871 1:-50663269 MsaT003023.1:CDS
AAAGGAGTGTCTAGAGAAAA+AGG 0.305223 1:-50664846 MsaT003023.1:CDS
CAGATTGATTCTCACAATTA+GGG 0.310638 1:+50663796 None:intergenic
AAAACCTGTGGCAGCATGTT+TGG 0.312351 1:-50667099 MsaT003023.1:CDS
CCTTGATTTAATAGATTTCT+GGG 0.316876 1:+50666719 None:intergenic
GAAGTTGGGTCTTCTATTTA+GGG 0.323812 1:+50663689 None:intergenic
AGAAGCTGGTCCTGCAATTC+AGG 0.327601 1:-50663583 MsaT003023.1:CDS
GTGGCAATGCCTGGTTTATC+AGG 0.332763 1:-50667385 MsaT003023.1:CDS
TGAAAAGTTTGATAACAATT+CGG 0.339114 1:-50666691 MsaT003023.1:CDS
GAAAAGTTTGATAACAATTC+GGG 0.346605 1:-50666690 MsaT003023.1:CDS
CAGCAAATGGATTGCAAGCT+TGG 0.350061 1:-50667456 MsaT003023.1:CDS
GATGTCATCTCATGACTCTA+TGG 0.359807 1:-50667194 MsaT003023.1:CDS
GACAGCAACAATGGACTTGC+TGG 0.359847 1:-50662779 MsaT003023.1:CDS
TTTACATATTAGATCCCTTA+AGG 0.361725 1:-50669535 MsaT003023.1:CDS
GAGCAAGATAATGGAAGTTT+AGG 0.362759 1:-50666657 MsaT003023.1:CDS
GTAACTTCTAAGCTGTTATT+AGG 0.363149 1:+50663361 None:intergenic
TCCCACTGAGAGCCTGATTT+AGG 0.367118 1:+50664959 None:intergenic
TGACAAGGGTTCTGCTACTC+AGG 0.372499 1:-50663056 MsaT003023.1:CDS
AATTTGCTCATTGAATTTAG+AGG 0.373603 1:+50663836 None:intergenic
TTGGTTGTTGATGCCTCTGC+TGG 0.385063 1:+50663199 None:intergenic
CTCAAGGGGCAATTATTTGT+TGG 0.386253 1:-50669566 MsaT003023.1:CDS
GCACTTCTATACAAGATTAT+GGG 0.386285 1:-50667358 MsaT003023.1:CDS
TTTAAGCTCATTCTTGCGTA+TGG 0.388945 1:+50667120 None:intergenic
CACTACCATCACCATTGTTC+AGG 0.390653 1:+50666634 None:intergenic
TTCCGTTCATCCTGAATTGC+AGG 0.392365 1:+50663573 None:intergenic
ACTCCACAAGCAAGACCTTA+AGG 0.392485 1:+50669520 None:intergenic
GTTGTCAATATCTGAGAAGT+TGG 0.394245 1:+50663674 None:intergenic
AGCAATGATGAGCAAGATAA+TGG 0.400711 1:-50666666 MsaT003023.1:CDS
CTTCACAATTGTAGTTATGA+AGG 0.400722 1:-50669462 MsaT003023.1:intron
TTCCCTGCATGTTTCAGCAT+TGG 0.406470 1:+50664889 None:intergenic
GCGGTTCCAGTCAAAATTGC+AGG 0.406686 1:-50663891 MsaT003023.1:intron
TGGAGTGACTGATGGAGTTA+AGG 0.413525 1:-50669649 MsaT003023.1:CDS
GCATCATTTGAGTTCTGGTT+AGG 0.415598 1:+50663289 None:intergenic
GTCAACTTCTACAGCTCGTT+TGG 0.415675 1:+50664985 None:intergenic
TCAAGGGGCAATTATTTGTT+GGG 0.420288 1:-50669565 MsaT003023.1:CDS
CATCATGCTCAGGTGGCAAC+TGG 0.423674 1:+50662984 None:intergenic
GTCTTTAAGTGTTTGTCAAA+GGG 0.426456 1:-50667166 MsaT003023.1:CDS
GAAGATTCGGCATCATGCTC+AGG 0.431544 1:+50662974 None:intergenic
GAAGCAACGCAGTGACAATA+TGG 0.433512 1:+50669481 None:intergenic
CTTTCTTTCCTTCAGTCTAG+TGG 0.435646 1:-50666768 MsaT003023.1:intron
ATCCCTTAAGGTCTTGCTTG+TGG 0.436804 1:-50669523 MsaT003023.1:CDS
TTAACTGAGGTGGCAATGCC+TGG 0.438214 1:-50667394 MsaT003023.1:CDS
TAAAATGAGATCAATATGAT+TGG 0.443706 1:+50667414 None:intergenic
GGAGTTAAGGTTGGTGAGGG+AGG 0.444636 1:-50669636 MsaT003023.1:CDS
ATAGGAGGTTGGATCTCCTT+AGG 0.445359 1:-50662028 MsaT003023.1:intron
TTCTACAGCTCGTTTGGTCC+AGG 0.448107 1:+50664991 None:intergenic
AACTTAAATGAAGGAGTAGC+TGG 0.450112 1:-50669696 MsaT003023.1:CDS
AATGGACTTGCTGGGAATAG+TGG 0.453499 1:-50662770 MsaT003023.1:CDS
TGGGCTGGAAAGCAGACGTT+TGG 0.453554 1:+50663167 None:intergenic
TTTCTTTCCTTCAGTCTAGT+GGG 0.456206 1:-50666767 MsaT003023.1:intron
TCTAGACACTCCTTTAAGAC+TGG 0.457847 1:+50664854 None:intergenic
TTCCTTCAGTCTAGTGGGAG+TGG 0.461821 1:-50666762 MsaT003023.1:intron
GAAGTTGACAGTCCTAAATC+AGG 0.462528 1:-50664971 MsaT003023.1:CDS
CTCCACAAGCAAGACCTTAA+GGG 0.463153 1:+50669521 None:intergenic
TCAGTTATTGAAGCAGCAAA+TGG 0.463336 1:-50667469 MsaT003023.1:intron
AGTATCAATAGAAGTGCTTC+AGG 0.463935 1:-50662869 MsaT003023.1:CDS
CCTAATTGTGAGAATCAATC+TGG 0.463977 1:-50663795 MsaT003023.1:CDS
AGTGCCAGTTGAAGGTAATA+CGG 0.464184 1:-50662900 MsaT003023.1:CDS
CTCTTGAGCTCAGTTTAAAG+AGG 0.474120 1:-50663620 MsaT003023.1:CDS
AGTGGTGATGCTAGTGGAAA+TGG 0.475297 1:-50662746 MsaT003023.1:CDS
TTCAATGAGCAAATTAATAG+AGG 0.478804 1:-50663828 MsaT003023.1:CDS
AGTCTTTAAGTGTTTGTCAA+AGG 0.479846 1:-50667167 MsaT003023.1:CDS
AAGGTTGGTGAGGGAGGGAA+TGG 0.483694 1:-50669630 MsaT003023.1:CDS
TTGTCAATATCTGAGAAGTT+GGG 0.484449 1:+50663675 None:intergenic
TTAAGCTCATTCTTGCGTAT+GGG 0.487300 1:+50667121 None:intergenic
TTTCCCGTATTACCTTCAAC+TGG 0.488361 1:+50662896 None:intergenic
AGTGGGAGTGGCAGTGAAAG+TGG 0.491905 1:-50666750 MsaT003023.1:CDS
ATTCCAATGCTGAAACATGC+AGG 0.495052 1:-50664892 MsaT003023.1:CDS
AGAGAGGTGCTTTCATAAGC+AGG 0.496751 1:-50662640 MsaT003023.1:intron
TCAAAACAAGAATTCTCAAA+GGG 0.500542 1:-50662705 MsaT003023.1:CDS
CCCAGAAATCTATTAAATCA+AGG 0.500800 1:-50666719 MsaT003023.1:CDS
AAAGCTCAAGCTCAAATACT+CGG 0.502200 1:+50661976 None:intergenic
AGCAGCGCTGCAATTAATGC+TGG 0.502976 1:-50662815 MsaT003023.1:CDS
GCTAGCAACAACATTGATAT+GGG 0.503896 1:-50663268 MsaT003023.1:CDS
GGGAGACTTCTTAGCATTGA+AGG 0.505204 1:+50663411 None:intergenic
TCCTCCAAACATGCTGCCAC+AGG 0.506809 1:+50667095 None:intergenic
GTGGTCAAAGTGCCAGTTGA+AGG 0.508886 1:-50662908 MsaT003023.1:CDS
ATTTGCTCATTGAATTTAGA+GGG 0.509233 1:+50663837 None:intergenic
GGTAAAAGCATTATTAGCAG+TGG 0.510774 1:+50663243 None:intergenic
ATGGAAGCAATGGACAAAAT+GGG 0.515017 1:-50662838 MsaT003023.1:CDS
ATGTAAGAGAGTAGAACCTA+AGG 0.517154 1:+50662012 None:intergenic
GCAAATTAATAGAGGACAGA+TGG 0.519442 1:-50663820 MsaT003023.1:CDS
TGAAACATGCAGGGAAAAGA+TGG 0.519924 1:-50664882 MsaT003023.1:CDS
AGAGTTGCTTCCGACACATC+CGG 0.520860 1:+50663387 None:intergenic
GGGCAATTATTTGTTGGGAG+AGG 0.522794 1:-50669560 MsaT003023.1:CDS
TCATTTGAGTTCTGGTTAGG+AGG 0.525127 1:+50663292 None:intergenic
GATGATGAAGTGAAATGTAA+TGG 0.525442 1:-50669669 MsaT003023.1:CDS
AAAGAACTTGGTCAGCGCTA+TGG 0.526757 1:-50669730 MsaT003023.1:CDS
AAAAGAAAGGAGAGAAAGAG+AGG 0.526805 1:-50662656 MsaT003023.1:CDS
GGAAGCAACAATGGAAGCAA+TGG 0.529487 1:-50662848 MsaT003023.1:CDS
GGGAAGAACAACTTAAATGA+AGG 0.530123 1:-50669705 MsaT003023.1:CDS
GAGTTAAGGTTGGTGAGGGA+GGG 0.530743 1:-50669635 MsaT003023.1:CDS
AACTTGAGCACAAGTGCTGT+CGG 0.532765 1:+50664919 None:intergenic
ATGTCATCTCATGACTCTAT+GGG 0.536687 1:-50667193 MsaT003023.1:CDS
CTGATCAGTCTGCTTTCTCA+AGG 0.536827 1:-50663536 MsaT003023.1:intron
CTTTGCATTTGAGAGTCAGA+AGG 0.538723 1:+50663726 None:intergenic
GTGCCAGTTGAAGGTAATAC+GGG 0.540221 1:-50662899 MsaT003023.1:CDS
CTGATTGTATCATAGGAGGT+TGG 0.540436 1:-50662039 MsaT003023.1:intron
AACTTGGTCAGCGCTATGGA+TGG 0.541522 1:-50669726 MsaT003023.1:CDS
TACCGCAGCAACAGTCTCAA+GGG 0.542656 1:-50669581 MsaT003023.1:CDS
ACTTGAGCACAAGTGCTGTC+GGG 0.543651 1:+50664920 None:intergenic
CCTAGAAAAGTTGAAGAAGC+TGG 0.545226 1:-50663597 MsaT003023.1:CDS
ACCTGTGGCAGCATGTTTGG+AGG 0.552529 1:-50667096 MsaT003023.1:CDS
ACATGTCAACAGACTTGATG+TGG 0.557814 1:-50663862 MsaT003023.1:CDS
GTACCGCAGCAACAGTCTCA+AGG 0.568297 1:-50669582 MsaT003023.1:CDS
ATCAAAACAAGAATTCTCAA+AGG 0.571810 1:-50662706 MsaT003023.1:CDS
AAATGTAATGGAGTGACTGA+TGG 0.573227 1:-50669657 MsaT003023.1:CDS
AAGCTCAAGCTCAAATACTC+GGG 0.581119 1:+50661977 None:intergenic
ACTTGGTCAGCGCTATGGAT+GGG 0.582844 1:-50669725 MsaT003023.1:CDS
TGACAATGGTAGTGGCACTC+AGG 0.582901 1:-50666613 MsaT003023.1:intron
GTCCTGCAATTCAGGATGAA+CGG 0.586027 1:-50663575 MsaT003023.1:CDS
GATGGAGTTAAGGTTGGTGA+GGG 0.589139 1:-50669639 MsaT003023.1:CDS
GTGACTGATGGAGTTAAGGT+TGG 0.591130 1:-50669645 MsaT003023.1:CDS
TCCTAAATCAGGCTCTCAGT+GGG 0.591373 1:-50664960 MsaT003023.1:CDS
AAGTCTCCCACCGGATGTGT+CGG 0.592788 1:-50663397 MsaT003023.1:CDS
AGTGCTTCAGGAAGCAACAA+TGG 0.594073 1:-50662857 MsaT003023.1:CDS
AGATACGACAACAACAATCC+TGG 0.594626 1:-50663092 MsaT003023.1:CDS
AGCGCTGCAATTAATGCTGG+AGG 0.596114 1:-50662812 MsaT003023.1:CDS
GCATGTTAGCAACATTGCTG+TGG 0.597791 1:+50663011 None:intergenic
AGTGCAATGCCTGATAAACC+AGG 0.599268 1:+50667376 None:intergenic
CTGTTGTTGCTCCACACTGT+GGG 0.599543 1:-50662940 MsaT003023.1:CDS
ACTGTTGTTGCTCCACACTG+TGG 0.604864 1:-50662941 MsaT003023.1:CDS
TTTGCATTTGAGAGTCAGAA+GGG 0.605063 1:+50663727 None:intergenic
TACTTGCTGCTGAACAGAAG+AGG 0.605168 1:+50669602 None:intergenic
GATGCTAGTGGAAATGGAAG+TGG 0.606551 1:-50662740 MsaT003023.1:CDS
TGATGGAGTTAAGGTTGGTG+AGG 0.607226 1:-50669640 MsaT003023.1:CDS
ACAAACATAGACAGCAACAA+TGG 0.609596 1:-50662788 MsaT003023.1:CDS
ACAGCAACAATGGACTTGCT+GGG 0.609766 1:-50662778 MsaT003023.1:CDS
CTTGCTGGGAATAGTGGCAG+TGG 0.611874 1:-50662764 MsaT003023.1:CDS
TTCCAATGCTGAAACATGCA+GGG 0.612716 1:-50664891 MsaT003023.1:CDS
GATATTCAATCTCATTCTAG+TGG 0.613980 1:-50663319 MsaT003023.1:CDS
TTTAAACTGAGCTCAAGAGA+TGG 0.614604 1:+50663624 None:intergenic
CAACGCAGTGACAATATGGC+GGG 0.615660 1:+50669485 None:intergenic
AATGGTGACTGTAAAGAACT+TGG 0.617086 1:-50669742 MsaT003023.1:CDS
GCCCCTTGAGACTGTTGCTG+CGG 0.622409 1:+50669579 None:intergenic
GATTCGGCATCATGCTCAGG+TGG 0.622530 1:+50662977 None:intergenic
ATGTGAGACAACTTGTTGAG+AGG 0.623924 1:+50663123 None:intergenic
AAATGAAGGAGTAGCTGGAG+AGG 0.626419 1:-50669691 MsaT003023.1:CDS
AGTGGCAGTGGTGATGCTAG+TGG 0.626932 1:-50662752 MsaT003023.1:CDS
GATGGTAGTGACAATGGTAG+TGG 0.629804 1:-50666621 MsaT003023.1:CDS
GTTGCTTCCGACACATCCGG+TGG 0.630609 1:+50663390 None:intergenic
ACTTCTAAGCTGTTATTAGG+CGG 0.632200 1:+50663364 None:intergenic
TTAGGCCTGAACAATGGTGA+TGG 0.633257 1:-50666639 MsaT003023.1:CDS
AGATTATGGGCCACCCAACA+CGG 0.633500 1:-50667345 MsaT003023.1:CDS
GGCACCACCTAAAATTGACA+AGG 0.635166 1:-50663071 MsaT003023.1:CDS
CAAAGGTACTAATAATGATG+AGG 0.638016 1:-50663652 MsaT003023.1:CDS
ACACTGTGGGTCATCAAATG+TGG 0.640190 1:-50662927 MsaT003023.1:CDS
AGACACTCCTTTAAGACTGG+AGG 0.640992 1:+50664857 None:intergenic
GCGCTGCAATTAATGCTGGA+GGG 0.641325 1:-50662811 MsaT003023.1:CDS
TTTGCTCATTGAATTTAGAG+GGG 0.646894 1:+50663838 None:intergenic
GTCCTAAATCAGGCTCTCAG+TGG 0.647285 1:-50664961 MsaT003023.1:CDS
AATGGTGATGGTAGTGACAA+TGG 0.650813 1:-50666627 MsaT003023.1:CDS
AGACTTCTTAGCATTGAAGG+TGG 0.657516 1:+50663414 None:intergenic
AGTTATAAGAGAGCCAGCAG+AGG 0.658218 1:-50663212 MsaT003023.1:CDS
TTCTCAGATATTGACAACAA+AGG 0.660645 1:-50663669 MsaT003023.1:CDS
AATGCTAAGAAGTCTCCCAC+CGG 0.662726 1:-50663406 MsaT003023.1:CDS
ACCGCAGCAACAGTCTCAAG+GGG 0.669084 1:-50669580 MsaT003023.1:CDS
GCAACGCAGTGACAATATGG+CGG 0.670206 1:+50669484 None:intergenic
GGAAGTTTAGGCCTGAACAA+TGG 0.671051 1:-50666645 MsaT003023.1:CDS
TTGCTTCCGACACATCCGGT+GGG 0.683631 1:+50663391 None:intergenic
CATGTCAACAGACTTGATGT+GGG 0.686791 1:-50663861 MsaT003023.1:CDS
ACATTTGATGACCCACAGTG+TGG 0.694810 1:+50662929 None:intergenic
TCTGACTCTCAAATGCAAAG+CGG 0.707947 1:-50663723 MsaT003023.1:CDS
CTTGTTGAGAGGTACACATG+AGG 0.720635 1:+50663134 None:intergenic
GCACCACCTAAAATTGACAA+GGG 0.725107 1:-50663070 MsaT003023.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 50661947 50669763 50661947 ID=MsaG003023
Chr1 mRNA 50661947 50669763 50661947 ID=MsaT003023.1;Parent=MsaG003023
Chr1 exon 50661947 50662046 50661947 ID=MsaT003023.1.exon8;Parent=MsaT003023.1
Chr1 CDS 50661947 50662046 50661947 ID=cds.MsaT003023.1;Parent=MsaT003023.1
Chr1 exon 50662641 50663444 50662641 ID=MsaT003023.1.exon7;Parent=MsaT003023.1
Chr1 CDS 50662641 50663444 50662641 ID=cds.MsaT003023.1;Parent=MsaT003023.1
Chr1 exon 50663537 50663903 50663537 ID=MsaT003023.1.exon6;Parent=MsaT003023.1
Chr1 CDS 50663537 50663903 50663537 ID=cds.MsaT003023.1;Parent=MsaT003023.1
Chr1 exon 50664837 50665017 50664837 ID=MsaT003023.1.exon5;Parent=MsaT003023.1
Chr1 CDS 50664837 50665017 50664837 ID=cds.MsaT003023.1;Parent=MsaT003023.1
Chr1 exon 50666614 50666775 50666614 ID=MsaT003023.1.exon4;Parent=MsaT003023.1
Chr1 CDS 50666614 50666775 50666614 ID=cds.MsaT003023.1;Parent=MsaT003023.1
Chr1 exon 50667084 50667217 50667084 ID=MsaT003023.1.exon3;Parent=MsaT003023.1
Chr1 CDS 50667084 50667217 50667084 ID=cds.MsaT003023.1;Parent=MsaT003023.1
Chr1 exon 50667326 50667487 50667326 ID=MsaT003023.1.exon2;Parent=MsaT003023.1
Chr1 CDS 50667326 50667487 50667326 ID=cds.MsaT003023.1;Parent=MsaT003023.1
Chr1 exon 50669463 50669763 50669463 ID=MsaT003023.1.exon1;Parent=MsaT003023.1
Chr1 CDS 50669463 50669763 50669463 ID=cds.MsaT003023.1;Parent=MsaT003023.1
Gene Sequence

>MsaT003023.1

ATGGTGACTGTAAAGAACTTGGTCAGCGCTATGGATGGGAAGAACAACTTAAATGAAGGAGTAGCTGGAGAGGATGATGAAGTGAAATGTAATGGAGTGACTGATGGAGTTAAGGTTGGTGAGGGAGGGAATGGCGAATCCTCTTCTGTTCAGCAGCAAGTACCGCAGCAACAGTCTCAAGGGGCAATTATTTGTTGGGAGAGGTTTTTACATATTAGATCCCTTAAGGTCTTGCTTGTGGAGTATGATGACTGTACCCGCCATATTGTCACTGCGTTGCTTCACAATTGTAGTTATGAAGTTATTGAAGCAGCAAATGGATTGCAAGCTTGGAAGATTTTGGAGGATTTAACCAATCATATTGATCTCATTTTAACTGAGGTGGCAATGCCTGGTTTATCAGGCATTGCACTTCTATACAAGATTATGGGCCACCCAACACGGAAAAACATTCCAGTAATTATGATGTCATCTCATGACTCTATGGGTTTAGTCTTTAAGTGTTTGTCAAAGGGTGCTGTCGACTTTCTAGTAAAACCCATACGCAAGAATGAGCTTAAAAACCTGTGGCAGCATGTTTGGAGGAGATGTCATAGTTCTAGTGGGAGTGGCAGTGAAAGTGGAACACAAACCCAGAAATCTATTAAATCAAGGAGTCTTGAAAAGTTTGATAACAATTCGGGAAGCAATGATGAGCAAGATAATGGAAGTTTAGGCCTGAACAATGGTGATGGTAGTGACAATGGTAGTGGCACTCAGAGCTCCTGGACCAAACGAGCTGTAGAAGTTGACAGTCCTAAATCAGGCTCTCAGTGGGATCAGATAGCTGAGTGTCCCGACAGCACTTGTGCTCAAGTTGTTCATTCCAATGCTGAAACATGCAGGGAAAAGATGGTTCCTCCAGTCTTAAAGGAGTGTCTAGAGAAAAAGGAACAAATAGTCAAAATTGCAGGTTCAAAACATGTCAACAGACTTGATGTGGGCCCCTCTAAATTCAATGAGCAAATTAATAGAGGACAGATGGACCCTAATTGTGAGAATCAATCTGGCAAACTAAGATGCAAAGTGCTTTCATTATCTGATGCAGTTACTAGCCCTTCTGACTCTCAAATGCAAAGCGGAGAAATTGAAGCCCTAAATAGAAGACCCAACTTCTCAGATATTGACAACAAAGGTACTAATAATGATGAGGAGTTGCCATCTCTTGAGCTCAGTTTAAAGAGGCCTAGAAAAGTTGAAGAAGCTGGTCCTGCAATTCAGGATGAACGGAACATTTTAAGACGTTCTGATCAGTCTGCTTTCTCAAGGTACAATGCCACCTTCAATGCTAAGAAGTCTCCCACCGGATGTGTCGGAAGCAACTCTCCGCCTAATAACAGCTTAGAAGTTACAAAGAAAGATTCATCTCGCGATATTCAATCTCATTCTAGTGGCAATCCTCCTAACCAGAACTCAAATGATGCTAGCAACAACATTGATATGGGTTCCACTGCTAATAATGCTTTTACCAAATCTGCAGTTATAAGAGAGCCAGCAGAGGCATCAACAACCAAATTTTTGTACCAAACGTCTGCTTTCCAGCCCATAAAAAACAACCTCATGTGTACCTCTCAACAAGTTGTCTCACATACTAATGAAGATACGACAACAACAATCCTGGCACCACCTAAAATTGACAAGGGTTCTGCTACTCAGGATTTGCATCACCATTATGAAAACCACAGCAATGTTGCTAACATGCAGCACCAGTTGCCACCTGAGCATGATGCCGAATCTTCAAAGAAAATGACTGTTGTTGCTCCACACTGTGGGTCATCAAATGTGGTCAAAGTGCCAGTTGAAGGTAATACGGGAAATTACAGTATCAATAGAAGTGCTTCAGGAAGCAACAATGGAAGCAATGGACAAAATGGGAGCAGCGCTGCAATTAATGCTGGAGGGACAAACATAGACAGCAACAATGGACTTGCTGGGAATAGTGGCAGTGGTGATGCTAGTGGAAATGGAAGTGGCAACAGAGTAGATCAAAACAAGAATTCTCAAAGGGAAGCAGCCCTAATTAAATTTCGCCAAAAAAGAAAGGAGAGAAAGAGAGGTGCTTTCATAAGCAGGAGGTTGGATCTCCTTAGGTTCTACTCTCTTACATATAAAAACTATTCCCGAGTATTTGAGCTTGAGCTTTTAGAAGAGTTGGAGATTGACAGAGTCTAA
Protein sequence

>MsaT003023.1

MVTVKNLVSAMDGKNNLNEGVAGEDDEVKCNGVTDGVKVGEGGNGESSSVQQQVPQQQSQGAIICWERFLHIRSLKVLLVEYDDCTRHIVTALLHNCSYEVIEAANGLQAWKILEDLTNHIDLILTEVAMPGLSGIALLYKIMGHPTRKNIPVIMMSSHDSMGLVFKCLSKGAVDFLVKPIRKNELKNLWQHVWRRCHSSSGSGSESGTQTQKSIKSRSLEKFDNNSGSNDEQDNGSLGLNNGDGSDNGSGTQSSWTKRAVEVDSPKSGSQWDQIAECPDSTCAQVVHSNAETCREKMVPPVLKECLEKKEQIVKIAGSKHVNRLDVGPSKFNEQINRGQMDPNCENQSGKLRCKVLSLSDAVTSPSDSQMQSGEIEALNRRPNFSDIDNKGTNNDEELPSLELSLKRPRKVEEAGPAIQDERNILRRSDQSAFSRYNATFNAKKSPTGCVGSNSPPNNSLEVTKKDSSRDIQSHSSGNPPNQNSNDASNNIDMGSTANNAFTKSAVIREPAEASTTKFLYQTSAFQPIKNNLMCTSQQVVSHTNEDTTTTILAPPKIDKGSATQDLHHHYENHSNVANMQHQLPPEHDAESSKKMTVVAPHCGSSNVVKVPVEGNTGNYSINRSASGSNNGSNGQNGSSAAINAGGTNIDSNNGLAGNSGSGDASGNGSGNRVDQNKNSQREAALIKFRQKRKERKRGAFISRRLDLLRFYSLTYKNYSRVFELELLEELEIDRV*