AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003075


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003075 MtrunA17_Chr3g0082121 32.075 477 275 11 65 526 57 499 9.12e-71 240
MsaG003075 MtrunA17_Chr2g0324011 29.783 507 317 10 47 538 3 485 1.12e-70 238
MsaG003075 MtrunA17_Chr1g0172971 76.515 132 16 1 1 132 1 117 1.74e-57 201
MsaG003075 MtrunA17_Chr7g0258861 34.139 331 190 7 46 371 2 309 7.84e-55 187
MsaG003075 MtrunA17_Chr1g0202341 28.755 466 296 9 53 506 53 494 7.20e-52 188
MsaG003075 MtrunA17_Chr8g0363841 27.328 494 321 9 56 536 22 490 4.67e-51 186
MsaG003075 MtrunA17_Chr2g0296781 27.695 538 322 15 55 558 1 505 3.30e-49 181
MsaG003075 MtrunA17_Chr1g0203721 24.441 581 359 15 21 555 11 557 7.30e-49 182
MsaG003075 MtrunA17_Chr1g0164591 31.579 323 190 7 147 454 1 307 4.14e-48 170
MsaG003075 MtrunA17_Chr5g0448761 26.626 492 325 10 53 529 62 532 1.53e-47 177
MsaG003075 MtrunA17_Chr6g0458391 26.070 514 312 12 57 551 231 695 5.30e-45 169
MsaG003075 MtrunA17_Chr5g0441581 25.731 513 321 13 39 536 25 492 1.53e-43 165
MsaG003075 MtrunA17_Chr6g0477261 31.562 320 204 5 196 504 7 322 4.64e-43 163
MsaG003075 MtrunA17_Chr6g0475591 95.890 73 3 0 204 276 1 73 6.24e-43 147
MsaG003075 MtrunA17_Chr2g0287651 25.755 497 325 11 21 502 45 512 6.96e-41 158
MsaG003075 MtrunA17_Chr2g0291591 25.726 482 283 14 53 505 66 501 4.33e-39 150
MsaG003075 MtrunA17_Chr1g0196541 25.647 464 300 12 55 504 1 433 1.41e-37 147
MsaG003075 MtrunA17_Chr1g0198761 25.981 535 344 16 37 546 33 540 9.87e-37 145
MsaG003075 MtrunA17_Chr1g0203051 22.887 485 342 6 66 542 94 554 1.06e-34 139
MsaG003075 MtrunA17_Chr5g0440451 24.476 572 354 18 1 542 1 524 1.72e-34 139
MsaG003075 MtrunA17_Chr2g0329031 25.311 482 308 15 33 502 3 444 1.92e-34 138
MsaG003075 MtrunA17_Chr2g0276841 24.547 497 330 13 65 542 84 554 3.16e-34 137
MsaG003075 MtrunA17_Chr2g0291581 24.609 447 286 9 54 488 74 481 6.59e-32 130
MsaG003075 MtrunA17_Chr5g0448511 29.720 286 184 7 232 505 1 281 1.59e-31 128
MsaG003075 MtrunA17_Chr1g0207551 23.967 484 321 11 44 513 32 482 2.44e-31 129
MsaG003075 MtrunA17_Chr3g0118611 28.358 335 217 10 237 558 111 435 2.11e-30 126
MsaG003075 MtrunA17_Chr5g0431001 25.482 467 307 13 29 475 39 484 2.86e-30 124
MsaG003075 MtrunA17_Chr2g0290391 24.151 530 342 16 45 546 44 541 3.63e-30 125
MsaG003075 MtrunA17_Chr2g0287061 25.251 499 293 16 57 542 189 620 4.56e-30 125
MsaG003075 MtrunA17_Chr3g0080351 23.670 545 341 15 15 535 44 537 1.01e-26 115
MsaG003075 MtrunA17_Chr2g0294841 24.283 453 286 14 48 473 42 464 1.80e-23 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003075 AT4G38180.1 26.371 474 300 11 52 505 74 518 9.82e-48 178
MsaG003075 AT4G38170.1 29.452 292 184 7 251 529 24 306 3.09e-37 145
MsaG003075 AT3G06250.2 24.948 477 280 11 39 504 178 587 5.97e-34 137
MsaG003075 AT3G06250.1 24.948 477 280 11 39 504 178 587 5.97e-34 137
MsaG003075 AT3G06250.3 24.948 477 280 11 39 504 178 587 5.97e-34 137
MsaG003075 AT4G15090.1 26.180 466 297 12 66 513 66 502 3.81e-32 132
MsaG003075 AT4G15090.5 26.180 466 297 12 66 513 66 502 3.81e-32 132
MsaG003075 AT4G15090.3 26.180 466 297 12 66 513 66 502 3.81e-32 132
MsaG003075 AT4G15090.2 26.180 466 297 12 66 513 66 502 3.81e-32 132
MsaG003075 AT4G15090.4 26.180 466 297 12 66 513 66 502 3.81e-32 132
MsaG003075 AT3G22170.2 22.978 544 355 13 1 529 32 526 7.17e-31 128
MsaG003075 AT3G22170.1 22.978 544 355 13 1 529 32 526 7.17e-31 128
MsaG003075 AT2G27110.3 28.472 288 188 7 229 504 36 317 1.52e-29 124
MsaG003075 AT2G27110.2 28.472 288 188 7 229 504 181 462 1.62e-29 124
MsaG003075 AT2G27110.1 28.472 288 188 7 229 504 181 462 1.62e-29 124
MsaG003075 AT1G10240.2 22.718 515 351 14 48 542 45 532 1.22e-28 121
MsaG003075 AT1G10240.1 22.718 515 351 14 48 542 45 532 1.22e-28 121
MsaG003075 AT5G28530.1 22.736 519 344 12 56 546 60 549 1.58e-28 120
MsaG003075 AT5G18960.2 23.810 441 258 13 37 463 197 573 1.61e-28 121
MsaG003075 AT5G18960.1 23.810 441 258 13 37 463 197 573 1.61e-28 121
MsaG003075 AT1G76320.3 24.026 462 303 12 66 513 12 439 3.44e-28 119
MsaG003075 AT5G28530.3 24.091 440 280 11 56 467 60 473 6.34e-28 119
MsaG003075 AT5G28530.2 24.091 440 280 11 56 467 60 473 6.34e-28 119
MsaG003075 AT1G76320.1 24.026 462 303 12 66 513 12 439 7.07e-28 119
MsaG003075 AT1G76320.4 24.026 462 303 12 66 513 12 439 7.07e-28 119
MsaG003075 AT1G76320.2 23.261 460 309 10 66 513 12 439 7.37e-28 119
MsaG003075 AT1G52520.1 24.667 450 282 14 57 490 91 499 1.51e-26 114
MsaG003075 AT4G19990.5 23.767 446 281 13 57 489 27 426 2.00e-24 108
MsaG003075 AT4G19990.6 27.612 268 177 6 235 489 163 426 2.07e-24 108
MsaG003075 AT4G19990.2 27.612 268 177 6 235 489 163 426 2.07e-24 108
MsaG003075 AT4G19990.1 27.612 268 177 6 235 489 106 369 2.12e-24 108
MsaG003075 AT4G19990.3 23.767 446 281 13 57 489 27 426 2.19e-24 108
MsaG003075 AT4G19990.4 23.767 446 281 13 57 489 27 426 2.19e-24 108
MsaG003075 AT4G19990.7 23.767 446 281 13 57 489 41 440 2.27e-24 108
MsaG003075 AT1G80010.1 25.000 468 292 17 57 499 101 534 4.36e-24 107
MsaG003075 AT2G32250.5 23.800 500 297 15 15 501 6 434 8.14e-24 106
MsaG003075 AT2G32250.2 23.800 500 297 15 15 501 6 434 1.30e-23 105
MsaG003075 AT2G32250.4 23.800 500 297 15 15 501 6 434 1.30e-23 105
MsaG003075 AT2G32250.6 23.800 500 297 15 15 501 6 434 1.42e-23 105
MsaG003075 AT2G32250.1 23.800 500 297 15 15 501 6 434 1.42e-23 105
MsaG003075 AT2G32250.3 23.800 500 297 15 15 501 6 434 1.42e-23 105
MsaG003075 AT2G32250.8 24.053 449 261 13 66 501 12 393 1.99e-23 105
MsaG003075 AT2G32250.7 24.053 449 261 13 66 501 12 393 2.06e-23 105

Find 65 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATTTCAGCTGCCTTTAT+TGG 0.156227 1:+51429019 MsaT003075.1:intron
CTTTAGTCATTTCAACATTA+TGG 0.194287 1:+51429565 MsaT003075.1:CDS
TAAATCGTCTGGTTTCTTAT+TGG 0.220826 1:-51429146 None:intergenic
TCTGATGTTGTCCTGTTGTT+AGG 0.288908 1:-51427849 None:intergenic
GGTAATTATGCATATAAAAC+TGG 0.302948 1:+51427705 MsaT003075.1:CDS
TTACTTCTCGAAATGATCTA+AGG 0.311482 1:-51428040 None:intergenic
CAACTAGATTATTCTCTATT+CGG 0.323831 1:+51428356 MsaT003075.1:CDS
GCCGATATTTATTGTTTGTT+CGG 0.341758 1:-51428406 None:intergenic
ATGATTGTCAAAACCAATAA+AGG 0.364908 1:-51429032 None:intergenic
GAAGGCCTACTTCAGACTTT+AGG 0.375686 1:+51429390 MsaT003075.1:CDS
GAGGGATAATCATGCTGAAT+TGG 0.376108 1:+51429598 MsaT003075.1:CDS
AACAATAAATATCGGCCATT+AGG 0.388497 1:+51428413 MsaT003075.1:CDS
ACCGAACAAACAATAAATAT+CGG 0.389395 1:+51428405 MsaT003075.1:CDS
GAGAACGTCTTATGAAGTTA+TGG 0.394356 1:+51428115 MsaT003075.1:CDS
TCATGCTGAATTGGACTTCA+AGG 0.399206 1:+51429607 MsaT003075.1:CDS
TTCCTTCAAATACTATTATG+AGG 0.399583 1:+51429739 MsaT003075.1:CDS
GCTCTTATAAATTCTAAAGT+TGG 0.400120 1:+51428083 MsaT003075.1:CDS
ATGTGTATAAGATATATAGA+TGG 0.407680 1:+51427970 MsaT003075.1:CDS
CGGTCTGTGTATAAATCGTC+TGG 0.420871 1:-51429157 None:intergenic
GAAGTTATGGGTCAAGGAGT+TGG 0.428685 1:+51428128 MsaT003075.1:CDS
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 1:+51428053 MsaT003075.1:CDS
ACAAAATAAGAAATTTGATA+AGG 0.431852 1:+51427767 MsaT003075.1:CDS
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 1:+51427918 MsaT003075.1:CDS
AACCGCATCAGCTGACTGTT+AGG 0.450316 1:-51429659 None:intergenic
TATGCATTGGTCTTCAGCTT+GGG 0.450920 1:+51429439 MsaT003075.1:CDS
TCCATGATACTTTATTGCTA+GGG 0.464004 1:-51427638 None:intergenic
AGAACGTCTTATGAAGTTAT+GGG 0.466325 1:+51428116 MsaT003075.1:CDS
GTTTGCTTAATCCTAACAAC+AGG 0.467124 1:+51427838 MsaT003075.1:CDS
GTATGCATTGGTCTTCAGCT+TGG 0.479582 1:+51429438 MsaT003075.1:CDS
CTTGCTTTGCATTTATTCAC+AGG 0.480587 1:+51429705 MsaT003075.1:CDS
TTTAAGTCTGAAAACCGAAA+AGG 0.483010 1:-51428167 None:intergenic
CTCCTCATAATAGTATTTGA+AGG 0.485089 1:-51429741 None:intergenic
TTCCATGATACTTTATTGCT+AGG 0.489989 1:-51427639 None:intergenic
ACATTATGGTTCAGAGAATG+AGG 0.494022 1:+51429579 MsaT003075.1:CDS
CGTTATGTTTGTAACAAACA+AGG 0.494754 1:+51427801 MsaT003075.1:CDS
AGCTGGAATATCTTTGAAGC+TGG 0.496270 1:+51427935 MsaT003075.1:CDS
CCCCTAGCAATAAAGTATCA+TGG 0.498381 1:+51427637 MsaT003075.1:CDS
TATGAGGAGGACACGACAAA+AGG 0.501557 1:+51429755 MsaT003075.1:CDS
CTAAGGTAACATGAATGCTC+TGG 0.504903 1:-51428023 None:intergenic
TAACAAACAAGGTTTCAAGA+AGG 0.511682 1:+51427812 MsaT003075.1:CDS
GAATGCTCTGGTTTATGTAA+TGG 0.517705 1:-51428011 None:intergenic
TTACAACCAACCCTTTCCTC+CGG 0.518116 1:-51427912 None:intergenic
TGTCTTCTGTAGCATCAACT+TGG 0.520245 1:-51428301 None:intergenic
TGTGTATAAGATATATAGAT+GGG 0.523615 1:+51427971 MsaT003075.1:CDS
ATGAGGAGGACACGACAAAA+GGG 0.528774 1:+51429756 MsaT003075.1:CDS
CATAATAGTATTTGAAGGAA+AGG 0.529686 1:-51429736 None:intergenic
TGGGCTTTGTTCATGGCACA+TGG 0.532868 1:+51429228 MsaT003075.1:CDS
GGCCTACTTCAGACTTTAGG+TGG 0.536366 1:+51429393 MsaT003075.1:CDS
AACAAACAAGGTTTCAAGAA+GGG 0.554561 1:+51427813 MsaT003075.1:CDS
TTCACAAACTCAATAACCAA+TGG 0.554620 1:-51427876 None:intergenic
TAAAGAATAGAACAAGTGAA+TGG 0.555459 1:-51429547 None:intergenic
GGAGTAACATTGAGAACTAT+CGG 0.556951 1:-51427616 None:intergenic
CTTACGAAAATCGTATGCAT+TGG 0.559732 1:+51429426 MsaT003075.1:CDS
CCATGATACTTTATTGCTAG+GGG 0.563054 1:-51427637 None:intergenic
CACGACAAAAGGGTCTGCGT+TGG 0.568588 1:+51429766 MsaT003075.1:CDS
GTTATGGGTCAAGGAGTTGG+AGG 0.578191 1:+51428131 MsaT003075.1:CDS
TTACTGATTCATGCTGAGAG+TGG 0.579535 1:+51427558 MsaT003075.1:CDS
TCTTATGAAGTTATGGGTCA+AGG 0.580120 1:+51428122 MsaT003075.1:CDS
TGAATAAATGCAAAGCAAGC+TGG 0.591761 1:-51429701 None:intergenic
ATCCTAACAGTCAGCTGATG+CGG 0.594920 1:+51429657 MsaT003075.1:CDS
GACAACATCAGACAGTCCAT+TGG 0.604607 1:+51427860 MsaT003075.1:CDS
CTTCAAATACTATTATGAGG+AGG 0.607664 1:+51429742 MsaT003075.1:CDS
ATATCAACTTCATCTGAGAG+TGG 0.614980 1:+51427513 MsaT003075.1:CDS
AGCCACCTAAAGTCTGAAGT+AGG 0.632459 1:-51429395 None:intergenic
CATTATGGTTCAGAGAATGA+GGG 0.643278 1:+51429580 MsaT003075.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 51427504 51429802 51427504 ID=MsaG003075
Chr1 mRNA 51427504 51429802 51427504 ID=MsaT003075.1;Parent=MsaG003075
Chr1 exon 51427504 51428434 51427504 ID=MsaT003075.1.exon1;Parent=MsaT003075.1
Chr1 CDS 51427504 51428434 51427504 ID=cds.MsaT003075.1;Parent=MsaT003075.1
Chr1 exon 51429029 51429181 51429029 ID=MsaT003075.1.exon2;Parent=MsaT003075.1
Chr1 CDS 51429029 51429181 51429029 ID=cds.MsaT003075.1;Parent=MsaT003075.1
Chr1 exon 51429210 51429802 51429210 ID=MsaT003075.1.exon3;Parent=MsaT003075.1
Chr1 CDS 51429210 51429802 51429210 ID=cds.MsaT003075.1;Parent=MsaT003075.1
Gene Sequence

>MsaT003075.1

ATGGATTTGATATCAACTTCATCTGAGAGTGGACAACTGAGTGTTGAAACTTCATTACTGATTCATGCTGAGAGTGGACAACTGAGTGTTGAAACTTCTTCAATGATTCATGCCGATAGTTCTCAATGTTACTCCCCTAGCAATAAAGTATCATGGAAGTCTTTAGTCTTTCATTCTATTAAAGAGATTGAAGATTTTTATGGTAATTATGCATATAAAACTGGATTTTCTATTAGGACTATGTTGAAGTCGAAGAATAATTCACAAAATAAGAAATTTGATAAGGTACACTATGTGCGTTATGTTTGTAACAAACAAGGTTTCAAGAAGGGAAGTTTGCTTAATCCTAACAACAGGACAACATCAGACAGTCCATTGGTTATTGAGTTTGTGAAAGTGAAAGAAAAACCGGAGGAAAGGGTTGGTTGTAAAGCTGGAATATCTTTGAAGCTGGATGAAGTTTTCAATGTGTATAAGATATATAGATGGGATGTAGCTCACTGTCATCCATTACATAAACCAGAGCATTCATGTTACCTTAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTAGCTCTTATAAATTCTAAAGTTGGAATGTCGATGAGAACGTCTTATGAAGTTATGGGTCAAGGAGTTGGAGGCACGAAGAATCTTCCTTTTCGGTTTTCAGACTTAAAGAACTATCTAATGACAATTCGTCAAAAAGAGATGGTGGTTGGTGAAGCAACTGTAATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCATCTTTTTACTATGATATCCAAGTTGATGCTACAGAAGACATAGCTAGTATTTTTTGGGCAGATGAAATTATGCAACTAGATTATTCTCTATTCGGCGATGTCATAAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGCTGCCTTTATTGGTTTTGACAATCATCGTAAGAGTGTTTTATTTGGTGTTGCGCTATTGTACGATGAGATAGCAGCTACTTTTGATTGGTTGTTTAAAACATTTTTGAAGTGTATGTCCAATAAGAAACCAGACGATTTATACACAGACCGAATTTTCCAGGTGTTTTTCATGGGCTTTGTTCATGGCACATGGCAGAGAATGCAAAGAAAAAGCTTGGCTCTCGTGCAAACAGTGCATTTTTTTGACGAGGTAACTAATTTGGTTTCAAATGTAGACGATGAATCAGATTTTGACTATAATTGGGATCAGATGATGAAAAATTGTTTTAATGGAAGGCCTACTTCAGACTTTAGGTGGCTTGCCCAAACTTACGAAAATCGTATGCATTGGTCTTCAGCTTGGGTCAAGTCACATTTTACAGCTGGTCTGAAAACGACTCAGTTAAGTGAGTCTTTCAATGCTTTTCTTTGTGGATTTCTGCAGTCAGACCATTCACTTGTTCTATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGAATTGGACTTCAAGGCTGCAAACAATAGAACAAAAAAAAATTATCCTAACAGTCAGCTGATGCGGTTCGTTGTAAACAAGTACACACCAGCTTGCTTTGCATTTATTCACAGGCAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACACGACAAAAGGGTCTGCGTTGGAGAAGTTTTTCTAA

Protein sequence

>MsaT003075.1

MDLISTSSESGQLSVETSLLIHAESGQLSVETSSMIHADSSQCYSPSNKVSWKSLVFHSIKEIEDFYGNYAYKTGFSIRTMLKSKNNSQNKKFDKVHYVRYVCNKQGFKKGSLLNPNNRTTSDSPLVIEFVKVKEKPEERVGCKAGISLKLDEVFNVYKIYRWDVAHCHPLHKPEHSCYLRSFREVNEVQGQLALINSKVGMSMRTSYEVMGQGVGGTKNLPFRFSDLKNYLMTIRQKEMVVGEATVIQEFFRNEALSKPSFYYDIQVDATEDIASIFWADEIMQLDYSLFGDVISFDTTYRTNNKYRPLAAFIGFDNHRKSVLFGVALLYDEIAATFDWLFKTFLKCMSNKKPDDLYTDRIFQVFFMGFVHGTWQRMQRKSLALVQTVHFFDEVTNLVSNVDDESDFDYNWDQMMKNCFNGRPTSDFRWLAQTYENRMHWSSAWVKSHFTAGLKTTQLSESFNAFLCGFLQSDHSLVLFFSHFNIMVQRMRDNHAELDFKAANNRTKKNYPNSQLMRFVVNKYTPACFAFIHRQYDLSFKYYYEEDTTKGSALEKFF*