AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003076

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003076 MtrunA17_Chr1g0181211 97.395 691 14 2 1 689 1 689 0.0 1394
MsaG003076 MtrunA17_Chr2g0326761 58.055 689 242 13 20 687 4 666 0.0 754
MsaG003076 MtrunA17_Chr4g0028971 59.130 690 223 13 24 687 9 665 0.0 737
MsaG003076 MtrunA17_Chr7g0264171 54.892 603 239 11 20 609 24 606 0.0 622
MsaG003076 MtrunA17_Chr1g0199681 50.000 644 241 12 1 602 76 680 0.0 568
MsaG003076 MtrunA17_Chr7g0264161 55.233 344 111 3 36 379 1 301 5.86e-125 373
MsaG003076 MtrunA17_Chr3g0115271 41.397 587 289 16 24 584 16 573 9.69e-118 365
MsaG003076 MtrunA17_Chr5g0424931 42.765 463 212 14 22 470 14 437 1.65e-103 326
MsaG003076 MtrunA17_Chr5g0417281 38.821 407 185 7 21 422 5 352 7.70e-87 282
MsaG003076 MtrunA17_Chr5g0436761 37.882 425 194 10 24 439 9 372 5.17e-80 262
MsaG003076 MtrunA17_Chr1g0178011 36.190 420 203 10 22 433 7 369 1.14e-79 261
MsaG003076 MtrunA17_Chr4g0009751 30.073 685 389 18 11 671 3 621 8.20e-79 263
MsaG003076 MtrunA17_Chr2g0289221 41.144 367 143 9 24 379 17 321 1.14e-78 255
MsaG003076 MtrunA17_Chr2g0282961 36.446 439 214 11 23 450 51 435 3.66e-77 259
MsaG003076 MtrunA17_Chr2g0276781 31.471 680 367 19 27 683 23 626 3.73e-76 258
MsaG003076 MtrunA17_Chr1g0156321 37.209 387 190 6 20 399 36 376 5.41e-76 261
MsaG003076 MtrunA17_Chr8g0385791 35.492 417 219 6 6 417 38 409 4.73e-75 258
MsaG003076 MtrunA17_Chr2g0326281 37.063 429 198 11 27 428 56 439 1.13e-72 251
MsaG003076 MtrunA17_Chr4g0029671 36.867 415 204 8 27 425 54 426 7.04e-72 248
MsaG003076 MtrunA17_Chr4g0046371 35.455 440 222 10 23 448 36 427 8.84e-72 246
MsaG003076 MtrunA17_Chr5g0417211 37.867 375 165 7 24 389 9 324 9.41e-72 238
MsaG003076 MtrunA17_Chr2g0277461 34.414 401 194 11 26 417 12 352 2.23e-71 239
MsaG003076 MtrunA17_Chr5g0433301 35.038 391 204 7 21 405 18 364 2.93e-70 245
MsaG003076 MtrunA17_Chr8g0376771 36.000 400 206 8 18 412 14 368 8.52e-70 244
MsaG003076 MtrunA17_Chr4g0070181 37.530 413 208 7 5 412 1 368 1.07e-69 246
MsaG003076 MtrunA17_Chr2g0285461 36.409 401 204 9 18 412 14 369 1.71e-68 241
MsaG003076 MtrunA17_Chr8g0389081 38.342 386 186 6 24 402 29 369 3.45e-68 242
MsaG003076 MtrunA17_Chr3g0109391 34.271 391 207 8 21 405 20 366 1.78e-65 232
MsaG003076 MtrunA17_Chr8g0346711 37.221 403 192 10 27 420 29 379 2.90e-64 225
MsaG003076 MtrunA17_Chr7g0239061 34.529 446 220 14 15 451 4 386 7.95e-64 224
MsaG003076 MtrunA17_Chr2g0301251 47.059 221 110 2 15 230 11 229 5.43e-58 212
MsaG003076 MtrunA17_Chr2g0301251 40.777 103 60 1 300 402 257 358 7.75e-17 85.1
MsaG003076 MtrunA17_Chr5g0424801 35.749 414 194 15 24 425 9 362 4.50e-57 198
MsaG003076 MtrunA17_Chr5g0425261 35.749 414 194 15 24 425 9 362 1.43e-56 197
MsaG003076 MtrunA17_Chr2g0277531 31.781 365 177 11 24 379 17 318 2.67e-55 191
MsaG003076 MtrunA17_Chr5g0424741 29.771 524 245 18 22 475 7 477 9.85e-50 182
MsaG003076 MtrunA17_Chr5g0425661 41.667 192 106 4 27 215 37 225 7.75e-47 166
MsaG003076 MtrunA17_Chr8g0356211 30.255 314 158 9 135 448 2 254 4.31e-37 141
MsaG003076 MtrunA17_Chr5g0425651 41.071 112 61 2 293 401 1 110 5.62e-19 83.6
MsaG003076 MtrunA17_Chr5g0424791 33.117 154 88 5 72 223 11 151 5.78e-17 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003076 AT4G30080.1 55.507 681 259 13 20 686 12 662 0.0 696
MsaG003076 AT2G28350.1 53.152 698 257 18 19 686 2 659 0.0 652
MsaG003076 AT2G28350.2 53.009 698 258 18 19 686 2 659 0.0 651
MsaG003076 AT1G77850.2 42.358 458 233 11 22 469 14 450 1.60e-113 354
MsaG003076 AT1G77850.1 42.576 458 232 12 22 469 14 450 2.58e-113 353
MsaG003076 AT1G59750.4 31.454 674 366 16 27 683 22 616 5.92e-80 268
MsaG003076 AT1G59750.1 31.642 670 375 14 27 683 22 621 1.02e-79 267
MsaG003076 AT1G59750.3 31.642 670 375 14 27 683 22 621 1.02e-79 267
MsaG003076 AT1G59750.2 31.493 670 373 15 27 683 22 618 7.56e-79 265
MsaG003076 AT1G19850.2 37.859 383 186 6 24 399 40 377 7.72e-76 261
MsaG003076 AT1G19850.1 37.859 383 186 6 24 399 51 388 7.73e-76 261
MsaG003076 AT2G33860.1 35.792 461 233 11 23 468 52 464 2.23e-75 254
MsaG003076 AT2G46530.1 29.872 703 335 22 2 683 3 568 1.21e-74 252
MsaG003076 AT5G60450.2 37.838 407 189 11 27 417 41 399 1.45e-74 256
MsaG003076 AT4G23980.1 29.985 677 367 18 27 683 12 601 2.30e-74 252
MsaG003076 AT5G60450.1 37.838 407 189 11 27 417 65 423 2.90e-74 255
MsaG003076 AT2G46530.3 30.236 678 322 21 27 683 42 589 6.58e-74 251
MsaG003076 AT5G37020.2 36.041 394 200 6 19 405 14 362 5.30e-73 251
MsaG003076 AT5G37020.1 36.041 394 200 6 19 405 14 362 1.52e-72 251
MsaG003076 AT4G23980.2 29.690 677 367 18 27 683 12 599 1.86e-72 247
MsaG003076 AT5G62000.2 34.346 428 223 7 2 417 30 411 1.00e-71 249
MsaG003076 AT5G62000.5 34.346 428 223 7 2 417 30 411 1.00e-71 249
MsaG003076 AT5G62000.3 34.346 428 223 7 2 417 30 411 1.00e-71 249
MsaG003076 AT5G62000.1 34.346 428 223 7 2 417 30 411 1.00e-71 249
MsaG003076 AT5G62000.4 34.346 428 223 7 2 417 30 411 1.34e-71 249
MsaG003076 AT3G61830.1 35.147 441 224 12 9 442 7 392 1.69e-70 241
MsaG003076 AT1G30330.1 35.910 401 206 9 18 412 14 369 1.09e-68 242
MsaG003076 AT1G30330.3 35.930 398 204 9 21 412 19 371 1.38e-68 242
MsaG003076 AT1G30330.2 35.930 398 204 9 21 412 19 371 1.38e-68 242
MsaG003076 AT5G20730.2 36.609 407 208 6 15 416 12 373 1.23e-66 238
MsaG003076 AT1G19220.1 36.855 407 207 6 15 416 11 372 1.38e-66 238
MsaG003076 AT5G20730.1 36.520 408 208 7 15 416 12 374 2.41e-65 234
MsaG003076 AT5G20730.3 36.520 408 208 7 15 416 12 374 2.63e-65 234
MsaG003076 AT1G35520.1 28.846 676 349 21 27 683 27 589 5.70e-62 218
MsaG003076 AT1G34410.1 28.251 669 362 19 27 683 27 589 7.09e-62 218
MsaG003076 AT1G34390.1 28.209 670 361 22 27 684 27 588 3.41e-59 210
MsaG003076 AT3G61830.2 35.925 373 183 10 71 442 24 341 1.99e-57 204
MsaG003076 AT1G34310.1 34.988 403 196 11 27 420 27 372 2.34e-57 205
MsaG003076 AT1G35240.1 34.048 420 210 11 27 437 27 388 2.67e-57 205
MsaG003076 AT1G35540.1 34.336 399 199 10 27 417 27 370 6.52e-57 204
MsaG003076 AT2G33860.2 36.467 351 169 8 127 468 2 307 5.02e-56 197
MsaG003076 AT2G46530.2 29.649 570 265 18 126 683 36 481 1.90e-54 195
MsaG003076 AT1G34170.1 32.587 402 204 11 27 418 25 369 1.87e-53 192
MsaG003076 AT5G37020.3 36.429 280 134 4 127 405 31 267 2.95e-48 181
MsaG003076 AT1G34170.2 31.095 402 184 11 27 418 25 343 5.57e-47 173
MsaG003076 AT1G34170.3 31.095 402 184 11 27 418 25 343 2.55e-46 173
MsaG003076 AT1G43950.1 41.346 208 102 7 27 226 27 222 3.56e-38 141
MsaG003076 ATMG00940.1 37.500 168 90 3 214 380 1 154 1.07e-23 98.6
MsaG003076 AT2G46870.1 34.862 109 64 2 116 224 24 125 2.80e-11 65.9
MsaG003076 AT3G25730.1 36.458 96 59 1 127 220 183 278 6.08e-11 65.1
MsaG003076 AT3G61970.1 27.670 206 120 7 119 303 4 201 7.35e-11 64.3

Find 184 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAAGATGAATATATTTA+TGG 0.118268 1:-51452728 None:intergenic
GTCTCGAATAAGTTGGTTTA+TGG 0.152750 1:-51451678 MsaT003076.1:CDS
AGATGGAAATACCAATAAAA+TGG 0.189014 1:-51450736 MsaT003076.1:CDS
GCATAAACCTCATCTGTTTC+AGG 0.203134 1:+51452466 None:intergenic
CCCTCCTCTTCAGAACATTT+TGG 0.208126 1:-51452239 MsaT003076.1:CDS
TGAGAAAGGTGAAAGGTTTA+TGG 0.219953 1:+51451196 None:intergenic
CGAAGAAACGGAAGCAAGTT+TGG 0.233645 1:-51450619 MsaT003076.1:CDS
TAAGCTTAAGCTTGTTCCTT+TGG 0.291537 1:-51452443 MsaT003076.1:CDS
TGGTTTATGGGAATTGTATC+TGG 0.292586 1:-51451665 MsaT003076.1:CDS
GAAACGATGGCACTGAAATT+TGG 0.305036 1:+51451120 None:intergenic
GGACAATTCATGAAGTTAAC+AGG 0.315804 1:+51452544 None:intergenic
TCGTTTCCTAGTAACCTTCT+AGG 0.317283 1:-51451104 MsaT003076.1:CDS
AGTTCAGGCAGGTCTATTTC+CGG 0.319570 1:-51450976 MsaT003076.1:CDS
TTCACCTTGGAGGCTTCTTC+AGG 0.328238 1:-51451609 MsaT003076.1:intron
AAGCTTAAGCTTGTTCCTTT+GGG 0.328422 1:-51452442 MsaT003076.1:CDS
AGAAGCCTCCAAGGTGAATT+AGG 0.335922 1:+51451614 None:intergenic
CTTGTTCCTTTGGGTGGAAA+TGG 0.343258 1:-51452433 MsaT003076.1:CDS
ATGCAGATTCGATGGTGTTC+TGG 0.343933 1:-51451737 MsaT003076.1:CDS
ACAATTGACCTTTCTTTACT+TGG 0.344982 1:-51450546 MsaT003076.1:CDS
GACAATCTCAATTTCTTCCT+TGG 0.347743 1:+51451170 None:intergenic
ACCGCTTGAAGGACCGCTTC+TGG 0.350997 1:+51451857 None:intergenic
TCTCGAATAAGTTGGTTTAT+GGG 0.357035 1:-51451677 MsaT003076.1:CDS
AATGTCTTTGCAAATGAAAC+AGG 0.359372 1:+51452352 None:intergenic
ATGGCGAGACGTGGAAGTTT+AGG 0.367612 1:-51452204 MsaT003076.1:CDS
CATGCCAGCAGGAGTACTTT+CGG 0.368965 1:+51451055 None:intergenic
TCATTATGGTCTTCCGATAT+CGG 0.371359 1:-51451015 MsaT003076.1:CDS
CATGAGGATCCATTACCATT+AGG 0.376847 1:-51452016 MsaT003076.1:CDS
CTCTCACTAGAGGAGCGTTT+TGG 0.379568 1:+51450666 None:intergenic
CTGGCTCCCTGCATGCCAGC+AGG 0.380532 1:+51451044 None:intergenic
TTCTTCCTTGGTTGTGAGAA+AGG 0.380986 1:+51451182 None:intergenic
CGCGGTTATCCTTATACCAT+TGG 0.383301 1:+51450640 None:intergenic
TGAGAGTTTCCAATGGTATA+AGG 0.391430 1:-51450649 MsaT003076.1:CDS
CCGCTAAAGTTACCGCTTGA+AGG 0.392870 1:+51451846 None:intergenic
CTGCTGGCATGCAGGGAGCC+AGG 0.396598 1:-51451043 MsaT003076.1:CDS
AACTTGCTTCCGTTTCTTCG+CGG 0.399377 1:+51450622 None:intergenic
AGTTTGTTGATCCCCTTCTC+TGG 0.400220 1:-51451637 MsaT003076.1:CDS
AACTCAGGCGCGAATATGAC+TGG 0.403386 1:-51451896 MsaT003076.1:CDS
AATCACAAGAAGCTTCTTGC+CGG 0.403468 1:-51452121 MsaT003076.1:CDS
AAGGAGGTTGACTATTCTAA+TGG 0.418815 1:-51450211 MsaT003076.1:CDS
GGAGCCAGGCATGCTCATTA+TGG 0.423939 1:-51451029 MsaT003076.1:CDS
TTTCGGGTAGACAACGGAAT+AGG 0.433921 1:+51451072 None:intergenic
TGGTATTTCCATCTGATGAA+TGG 0.434165 1:+51450745 None:intergenic
GGCTAATAAGCAACCGTTTG+AGG 0.435944 1:-51451825 MsaT003076.1:CDS
CCAACAGGATTCCACCCTAA+TGG 0.436228 1:+51452001 None:intergenic
AGAAAGCTGGCAGACATGTT+TGG 0.443426 1:-51450504 MsaT003076.1:CDS
GTGCTCTACCGTGATATATC+TGG 0.445579 1:-51450453 MsaT003076.1:CDS
GGAATCAAAGGTGAAACCTT+TGG 0.447087 1:+51452523 None:intergenic
TACACAGAATGAGAATCAAA+TGG 0.448127 1:-51451954 MsaT003076.1:CDS
ATGAGGATCCATTACCATTA+GGG 0.450034 1:-51452015 MsaT003076.1:CDS
TCTGGCCTAATTCACCTTGG+AGG 0.451766 1:-51451619 MsaT003076.1:CDS
TCTCCACCTCAGTAAAGTTC+AGG 0.453129 1:-51450991 MsaT003076.1:CDS
ACACAGAATGAGAATCAAAT+GGG 0.454771 1:-51451953 MsaT003076.1:CDS
TAAGGATAACCGCGAAGAAA+CGG 0.457301 1:-51450631 MsaT003076.1:CDS
ATGATCAAAAGGCACGAAAC+CGG 0.458029 1:+51450953 None:intergenic
GTCATTAGAGATTTGTTGCT+CGG 0.460103 1:+51450767 None:intergenic
GTTTGTGATTCTTACCTGAA+TGG 0.461365 1:+51450405 None:intergenic
GATGGAAATACCAATAAAAT+GGG 0.463679 1:-51450735 MsaT003076.1:CDS
CTGAGGTGGAGATCCGATAT+CGG 0.464440 1:+51451002 None:intergenic
ATGCCAGCAGGAGTACTTTC+GGG 0.465859 1:+51451056 None:intergenic
TGGGATTCGACGAGCGAAGA+AGG 0.469310 1:-51452053 MsaT003076.1:CDS
CACTGAAATTTGGCCGTCGA+GGG 0.472008 1:+51451130 None:intergenic
ATAGGTTGCTCTGACCTAGA+AGG 0.479580 1:+51451090 None:intergenic
TGCATTGCCGCCTCTACTAA+AGG 0.482510 1:+51451755 None:intergenic
CAACTATGGCATGCTTGTGC+AGG 0.483052 1:-51452643 MsaT003076.1:CDS
GGATTCCACCCTAATGGTAA+TGG 0.485472 1:+51452007 None:intergenic
ATCACAAGAAGCTTCTTGCC+GGG 0.486243 1:-51452120 MsaT003076.1:CDS
AAGTACTCCTGCTGGCATGC+AGG 0.486614 1:-51451051 MsaT003076.1:CDS
AAAATGGTGATCTTTGTGTT+GGG 0.488407 1:-51452072 MsaT003076.1:CDS
TTAATAGATAAAACTTTGCA+TGG 0.488914 1:+51452502 None:intergenic
AGACGAATCCTCGGTTTCAA+AGG 0.490672 1:+51451697 None:intergenic
CGACGAGCGAAGAAGGGTAT+TGG 0.491306 1:-51452046 MsaT003076.1:CDS
TTCTCTGGCCTAATTCACCT+TGG 0.492130 1:-51451622 MsaT003076.1:CDS
CCTTTGGGTGGAAATGGTAA+TGG 0.496376 1:-51452427 MsaT003076.1:CDS
AAAACTTTGCATGGAATCAA+AGG 0.504526 1:+51452511 None:intergenic
CTTAAGCTTGTTCCTTTGGG+TGG 0.509650 1:-51452439 MsaT003076.1:CDS
ATTTACTGCAAAATGTAAGA+AGG 0.509927 1:-51451259 MsaT003076.1:CDS
AGTTTAGCAGCATGATCAAA+AGG 0.510146 1:+51450942 None:intergenic
CTCTGACCTAGAAGGTTACT+AGG 0.513995 1:+51451098 None:intergenic
AAGTTTAGGCATATTTATAG+AGG 0.517507 1:-51452190 MsaT003076.1:CDS
TGTCTCTTGCTTACTATCCA+TGG 0.518182 1:-51450868 MsaT003076.1:CDS
CCATTAGGGTGGAATCCTGT+TGG 0.519653 1:-51452001 MsaT003076.1:CDS
GGAAGCAAGTTTGGAGAGTA+CGG 0.521728 1:-51450610 MsaT003076.1:CDS
TGACTGGGAGGGAAAAGGTT+AGG 0.521859 1:-51451880 MsaT003076.1:CDS
TAAACTTTACTGTTCACTGT+TGG 0.522539 1:+51452604 None:intergenic
TGATGAACTGTACAGAAAGC+TGG 0.523736 1:-51450517 MsaT003076.1:CDS
GGTGTTTAGATCCTCAACTA+TGG 0.524660 1:-51452657 MsaT003076.1:CDS
GGGTAGTAAATAACCTCAAA+CGG 0.525195 1:+51451812 None:intergenic
CATAAGATCCAAGTAAAGAA+AGG 0.525657 1:+51450538 None:intergenic
ACGAGGAGTTCCCGAACCAT+CGG 0.527122 1:+51450704 None:intergenic
GCCAAAATGTTCTGAAGAGG+AGG 0.527805 1:+51452238 None:intergenic
TTCTGTGTAAACCAAGGAAG+AGG 0.532226 1:+51451968 None:intergenic
GAATATGACTGGGAGGGAAA+AGG 0.532595 1:-51451885 MsaT003076.1:CDS
ATGTAAGAAGGGTGAGTCCA+TGG 0.533350 1:-51451247 MsaT003076.1:CDS
AGGATTCGTCTCGAATAAGT+TGG 0.533362 1:-51451685 MsaT003076.1:CDS
GAAAATGGTGATCTTTGTGT+TGG 0.533797 1:-51452073 MsaT003076.1:CDS
ACATTGGAGAGGAACCATTC+AGG 0.535436 1:-51450419 MsaT003076.1:intron
TCTAATGACCATTCATCAGA+TGG 0.537635 1:-51450753 MsaT003076.1:CDS
AGTACTCCTGCTGGCATGCA+GGG 0.540145 1:-51451050 MsaT003076.1:CDS
AAGACCATAATGAGCATGCC+TGG 0.540953 1:+51451025 None:intergenic
GCATGCTTGTGCAGGTGGAA+TGG 0.542227 1:-51452635 MsaT003076.1:CDS
CCTTCAAGCGGTAACTTTAG+CGG 0.549169 1:-51451846 MsaT003076.1:CDS
GCGAAGAAGGGTATTGGTGG+TGG 0.550842 1:-51452040 MsaT003076.1:CDS
GGGATTCGACGAGCGAAGAA+GGG 0.551807 1:-51452052 MsaT003076.1:CDS
AAAGTTTATTACTTTCCTCA+AGG 0.553230 1:-51452589 MsaT003076.1:CDS
CTATGGCATGCTTGTGCAGG+TGG 0.553241 1:-51452640 MsaT003076.1:CDS
CTACCCGAAAGTACTCCTGC+TGG 0.554091 1:-51451059 MsaT003076.1:CDS
TGTGAAGGCGCCTTTAGTAG+AGG 0.555008 1:-51451765 MsaT003076.1:CDS
AAGGTGAATTAGGCCAGAGA+AGG 0.557096 1:+51451624 None:intergenic
TAACTTCATGAATTGTCCAA+AGG 0.561840 1:-51452539 MsaT003076.1:CDS
AATAACAATGGTTTGAACTC+AGG 0.561882 1:-51451911 MsaT003076.1:CDS
AACTCTGGCGTACTTGCGCG+AGG 0.565767 1:+51451791 None:intergenic
TTTACTGCAAAATGTAAGAA+GGG 0.567494 1:-51451258 MsaT003076.1:CDS
TTAGCCAAAATGTTCTGAAG+AGG 0.569408 1:+51452235 None:intergenic
GCACTGAAATTTGGCCGTCG+AGG 0.570322 1:+51451129 None:intergenic
CCATTACCATTTCCACCCAA+AGG 0.573446 1:+51452427 None:intergenic
CAAGATGGCCTTTGAAACCG+AGG 0.577088 1:-51451705 MsaT003076.1:CDS
CGAGCGAAGAAGGGTATTGG+TGG 0.577713 1:-51452043 MsaT003076.1:CDS
GCTACTAATTCAACCAACCA+TGG 0.579195 1:+51451230 None:intergenic
ATTCATCAGAAGAAGTTGAA+AGG 0.580416 1:-51452678 MsaT003076.1:CDS
AGGTGAATTAGGCCAGAGAA+GGG 0.583510 1:+51451625 None:intergenic
ATTAACAACAACAATAACAA+TGG 0.588569 1:-51451923 MsaT003076.1:CDS
CACCTCAGTAAAGTTCAGGC+AGG 0.589149 1:-51450987 MsaT003076.1:CDS
CTGCCTGAACTTTACTGAGG+TGG 0.589800 1:+51450988 None:intergenic
AAAGGTGAAAGGTTTATGGA+TGG 0.590026 1:+51451200 None:intergenic
CTGAAATTTGGCCGTCGAGG+GGG 0.590791 1:+51451132 None:intergenic
TCAAAAGGCACGAAACCGGC+CGG 0.591651 1:+51450957 None:intergenic
GGCGCCTTCACACAAAACTC+TGG 0.592521 1:+51451776 None:intergenic
CAGGCAGGTCTATTTCCGGC+CGG 0.592530 1:-51450972 MsaT003076.1:CDS
GTAGTCAAAAGATGCCGCCT+CGG 0.596640 1:+51452163 None:intergenic
AAGATGTTCATGGCGAGACG+TGG 0.596686 1:-51452213 MsaT003076.1:CDS
AGGCGGCAATGCAGATTCGA+TGG 0.597819 1:-51451745 MsaT003076.1:CDS
TCTGATGCTAACAATGGTGG+AGG 0.600675 1:-51452322 MsaT003076.1:CDS
GTTACCTGAAGAAGCCTCCA+AGG 0.601497 1:+51451605 None:intergenic
GCTGCAGATTATGACACTGT+TGG 0.602356 1:-51452397 MsaT003076.1:CDS
TTCTGGAATAAGATTCAAGA+TGG 0.603114 1:-51451720 MsaT003076.1:CDS
GGGTATTGGTGGTGGACATG+AGG 0.604425 1:-51452032 MsaT003076.1:CDS
TTCTCATTCTGTGTAAACCA+AGG 0.607875 1:+51451962 None:intergenic
GCAGATTATGACACTGTTGG+AGG 0.608851 1:-51452394 MsaT003076.1:CDS
TGGCCGAAAAGAACAAGCTG+AGG 0.609518 1:+51450801 None:intergenic
TTGGTTGTGAGAAAGGTGAA+AGG 0.610407 1:+51451189 None:intergenic
CAATCTGATGCTAACAATGG+TGG 0.611120 1:-51452325 MsaT003076.1:CDS
GCATGTTCAGCATGACCTTG+AGG 0.617891 1:+51452574 None:intergenic
TCACAAGAAGCTTCTTGCCG+GGG 0.620934 1:-51452119 MsaT003076.1:CDS
TCTGGAGCAGTCAAACACAT+TGG 0.623032 1:-51450435 MsaT003076.1:CDS
ACTTATTCGAGACGAATCCT+CGG 0.623357 1:+51451688 None:intergenic
AATGTTCTGAAGAGGAGGGT+CGG 0.624657 1:+51452243 None:intergenic
AGACTGAGTAGAACTTGCCA+TGG 0.625027 1:+51450851 None:intergenic
GCACAAGCATGCCATAGTTG+AGG 0.626456 1:+51452646 None:intergenic
GGTAAGACTTGTTGATGACG+AGG 0.626525 1:+51450687 None:intergenic
ACTGAAATTTGGCCGTCGAG+GGG 0.629416 1:+51451131 None:intergenic
TTTCCCTCGTTTGGATTACG+CGG 0.631927 1:-51452266 MsaT003076.1:CDS
AGGATCCATTACCATTAGGG+TGG 0.632365 1:-51452012 MsaT003076.1:CDS
GCCAGAAGCGGTCCTTCAAG+CGG 0.635688 1:-51451858 MsaT003076.1:CDS
TTTCTCGAATCAGAAGATGT+CGG 0.638567 1:-51450573 MsaT003076.1:CDS
GGCTGATCCTGAAACAGATG+AGG 0.642209 1:-51452473 MsaT003076.1:CDS
TCGAATCAGAAGATGTCGGT+CGG 0.645828 1:-51450569 MsaT003076.1:CDS
ACTCAGGCGCGAATATGACT+GGG 0.647010 1:-51451895 MsaT003076.1:CDS
AGGCGCGAATATGACTGGGA+GGG 0.648683 1:-51451891 MsaT003076.1:CDS
TTTCACCTTTCTCACAACCA+AGG 0.649147 1:-51451187 MsaT003076.1:CDS
GGAAAAGGTTAGGCCAGAAG+CGG 0.650274 1:-51451870 MsaT003076.1:CDS
TGACTGCTCCAGATATATCA+CGG 0.657225 1:+51450445 None:intergenic
CCAAAATGTTCTGAAGAGGA+GGG 0.662547 1:+51452239 None:intergenic
ATCGTCTCAGCACAATACCT+AGG 0.664599 1:+51452289 None:intergenic
CCATTGGAAACTCTCACTAG+AGG 0.665637 1:+51450656 None:intergenic
CCTCTAGTGAGAGTTTCCAA+TGG 0.667381 1:-51450656 MsaT003076.1:CDS
ACTCAATCTGATGCTAACAA+TGG 0.675509 1:-51452328 MsaT003076.1:CDS
ACTCTGGCGTACTTGCGCGA+GGG 0.681238 1:+51451792 None:intergenic
AAGAAGGGTGAGTCCATGGT+TGG 0.682554 1:-51451243 MsaT003076.1:CDS
CAGGCGCGAATATGACTGGG+AGG 0.684044 1:-51451892 MsaT003076.1:CDS
GAGTACTTTCGGGTAGACAA+CGG 0.687017 1:+51451066 None:intergenic
ATTAGGTTATTAGAGACACT+AGG 0.687638 1:+51450918 None:intergenic
GGTGAATTAGGCCAGAGAAG+GGG 0.693414 1:+51451626 None:intergenic
AGCAGTCAAACACATTGGAG+AGG 0.695616 1:-51450430 MsaT003076.1:CDS
CGGCCGCGTAATCCAAACGA+GGG 0.702235 1:+51452263 None:intergenic
AGAAGGTTACTAGGAAACGA+TGG 0.704217 1:+51451107 None:intergenic
GAAGGCGCCTTTAGTAGAGG+CGG 0.708805 1:-51451762 MsaT003076.1:CDS
CACAGAATGAGAATCAAATG+GGG 0.710437 1:-51451952 MsaT003076.1:CDS
GGTCATGCTGAACATGCATG+TGG 0.714823 1:-51452568 MsaT003076.1:CDS
TTTATAGAGGAACACCGAGG+CGG 0.715637 1:-51452177 MsaT003076.1:CDS
GACCTGCCTGAACTTTACTG+AGG 0.725533 1:+51450985 None:intergenic
TCGGCCGCGTAATCCAAACG+AGG 0.730598 1:+51452262 None:intergenic
GTAACTTCACAAGAACAGCA+AGG 0.734687 1:-51450230 MsaT003076.1:CDS
ACTTCACAAGAACAGCAAGG+AGG 0.777122 1:-51450227 MsaT003076.1:CDS
ATATTTATAGAGGAACACCG+AGG 0.862150 1:-51452180 MsaT003076.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 51450197 51452743 51450197 ID=MsaG003076
Chr1 mRNA 51450197 51452743 51450197 ID=MsaT003076.1;Parent=MsaG003076
Chr1 exon 51450197 51450251 51450197 ID=MsaT003076.1.exon3;Parent=MsaT003076.1
Chr1 CDS 51450197 51450251 51450197 ID=cds.MsaT003076.1;Parent=MsaT003076.1
Chr1 exon 51450420 51451300 51450420 ID=MsaT003076.1.exon2;Parent=MsaT003076.1
Chr1 CDS 51450420 51451300 51450420 ID=cds.MsaT003076.1;Parent=MsaT003076.1
Chr1 exon 51451610 51452743 51451610 ID=MsaT003076.1.exon1;Parent=MsaT003076.1
Chr1 CDS 51451610 51452743 51451610 ID=cds.MsaT003076.1;Parent=MsaT003076.1
Gene Sequence

>MsaT003076.1

ATGAATATATTTATGGATTCAAGAGATAATAACAATAATAACAATTCATCAGAAGAAGTTGAAAGGTGTTTAGATCCTCAACTATGGCATGCTTGTGCAGGTGGAATGGTTCAGATGCCAACAGTGAACAGTAAAGTTTATTACTTTCCTCAAGGTCATGCTGAACATGCATGTGGACCTGTTAACTTCATGAATTGTCCAAAGGTTTCACCTTTGATTCCATGCAAAGTTTTATCTATTAAGTATATGGCTGATCCTGAAACAGATGAGGTTTATGCTAAGCTTAAGCTTGTTCCTTTGGGTGGAAATGGTAATGGAAATGATGCTGCAGATTATGACACTGTTGGAGGAAATGAAAATCAAGATAAGCCTGTTTCATTTGCAAAGACATTGACTCAATCTGATGCTAACAATGGTGGAGGTTTTTCTGTTCCTAGGTATTGTGCTGAGACGATTTTCCCTCGTTTGGATTACGCGGCCGACCCTCCTCTTCAGAACATTTTGGCTAAAGATGTTCATGGCGAGACGTGGAAGTTTAGGCATATTTATAGAGGAACACCGAGGCGGCATCTTTTGACTACTGGTTGGAGTACTTTTGTTAATCACAAGAAGCTTCTTGCCGGGGATTCGATTGTGTTTTTGAGAGCGGAAAATGGTGATCTTTGTGTTGGGATTCGACGAGCGAAGAAGGGTATTGGTGGTGGACATGAGGATCCATTACCATTAGGGTGGAATCCTGTTGGAGAAAATTTTCCTCTTCCTTGGTTTACACAGAATGAGAATCAAATGGGGAGAAACATTAACAACAACAATAACAATGGTTTGAACTCAGGCGCGAATATGACTGGGAGGGAAAAGGTTAGGCCAGAAGCGGTCCTTCAAGCGGTAACTTTAGCGGCTAATAAGCAACCGTTTGAGGTTATTTACTACCCTCGCGCAAGTACGCCAGAGTTTTGTGTGAAGGCGCCTTTAGTAGAGGCGGCAATGCAGATTCGATGGTGTTCTGGAATAAGATTCAAGATGGCCTTTGAAACCGAGGATTCGTCTCGAATAAGTTGGTTTATGGGAATTGTATCTGGAGTTCAGTTTGTTGATCCCCTTCTCTGGCCTAATTCACCTTGGAGGCTTCTTCAGGTTAAATGGGATGAACCAGATTTACTGCAAAATGTAAGAAGGGTGAGTCCATGGTTGGTTGAATTAGTAGCAAACATTCCATCCATAAACCTTTCACCTTTCTCACAACCAAGGAAGAAATTGAGATTGTCTCCACACCACGATTTCCCCCTCGACGGCCAAATTTCAGTGCCATCGTTTCCTAGTAACCTTCTAGGTCAGAGCAACCTATTCCGTTGTCTACCCGAAAGTACTCCTGCTGGCATGCAGGGAGCCAGGCATGCTCATTATGGTCTTCCGATATCGGATCTCCACCTCAGTAAAGTTCAGGCAGGTCTATTTCCGGCCGGTTTCGTGCCTTTTGATCATGCTGCTAAACTACCTAGTGTCTCTAATAACCTAATGATGCAAAAATCTAGCACGAGTGAGAATGTCTCTTGCTTACTATCCATGGCAAGTTCTACTCAGTCTTCAAAGAAATTCAACGATGAGAAGAAACCTCAGCTTGTTCTTTTCGGCCAAAAAATTCTCACCGAGCAACAAATCTCTAATGACCATTCATCAGATGGAAATACCAATAAAATGGGTAATTTTTCCGATGGTTCGGGAACTCCTCGTCATCAACAAGTCTTACCAAAACGCTCCTCTAGTGAGAGTTTCCAATGGTATAAGGATAACCGCGAAGAAACGGAAGCAAGTTTGGAGAGTACGGATCACTGTAAAGTTTTTCTCGAATCAGAAGATGTCGGTCGGACAATTGACCTTTCTTTACTTGGATCTTATGATGAACTGTACAGAAAGCTGGCAGACATGTTTGGCATAGAAAAATATGAGAAGGTAAGTCGCGTGCTCTACCGTGATATATCTGGAGCAGTCAAACACATTGGAGAGGAACCATTCAGTAACTTCACAAGAACAGCAAGGAGGTTGACTATTCTAATGGATTCAAACAAATAA
Protein sequence

>MsaT003076.1

MNIFMDSRDNNNNNNSSEEVERCLDPQLWHACAGGMVQMPTVNSKVYYFPQGHAEHACGPVNFMNCPKVSPLIPCKVLSIKYMADPETDEVYAKLKLVPLGGNGNGNDAADYDTVGGNENQDKPVSFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYAADPPLQNILAKDVHGETWKFRHIYRGTPRRHLLTTGWSTFVNHKKLLAGDSIVFLRAENGDLCVGIRRAKKGIGGGHEDPLPLGWNPVGENFPLPWFTQNENQMGRNINNNNNNGLNSGANMTGREKVRPEAVLQAVTLAANKQPFEVIYYPRASTPEFCVKAPLVEAAMQIRWCSGIRFKMAFETEDSSRISWFMGIVSGVQFVDPLLWPNSPWRLLQVKWDEPDLLQNVRRVSPWLVELVANIPSINLSPFSQPRKKLRLSPHHDFPLDGQISVPSFPSNLLGQSNLFRCLPESTPAGMQGARHAHYGLPISDLHLSKVQAGLFPAGFVPFDHAAKLPSVSNNLMMQKSSTSENVSCLLSMASSTQSSKKFNDEKKPQLVLFGQKILTEQQISNDHSSDGNTNKMGNFSDGSGTPRHQQVLPKRSSSESFQWYKDNREETEASLESTDHCKVFLESEDVGRTIDLSLLGSYDELYRKLADMFGIEKYEKVSRVLYRDISGAVKHIGEEPFSNFTRTARRLTILMDSNK*