AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003098


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003098 MtrunA17_Chr1g0180911 93.720 207 10 3 1 206 1 205 4.81e-102 293
MsaG003098 MtrunA17_Chr7g0224581 60.577 104 36 2 15 118 20 118 1.15e-36 129
MsaG003098 MtrunA17_Chr8g0350941 78.378 74 16 0 49 122 79 152 2.83e-35 127
MsaG003098 MtrunA17_Chr2g0277361 60.000 100 29 1 14 113 105 193 2.85e-35 129
MsaG003098 MtrunA17_Chr4g0060601 73.171 82 19 1 31 112 81 159 4.30e-35 128
MsaG003098 MtrunA17_Chr5g0416581 73.684 76 20 0 49 124 66 141 8.25e-35 126
MsaG003098 MtrunA17_Chr1g0211941 72.500 80 17 2 34 112 20 95 1.58e-34 123
MsaG003098 MtrunA17_Chr1g0164881 68.235 85 21 2 39 121 25 105 4.01e-34 121
MsaG003098 MtrunA17_Chr1g0204001 73.077 78 21 0 45 122 59 136 1.31e-33 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003098 AT2G37000.1 55.556 144 46 4 50 192 43 169 5.41e-40 135
MsaG003098 AT1G58100.1 55.932 118 41 1 48 154 58 175 8.80e-36 130
MsaG003098 AT1G69690.1 67.816 87 25 2 28 113 31 115 1.21e-35 128
MsaG003098 AT1G72010.1 72.973 74 20 0 39 112 52 125 7.09e-35 127
MsaG003098 AT5G08330.1 76.316 76 14 1 50 121 31 106 8.34e-35 123
MsaG003098 AT3G47620.1 76.000 75 17 1 39 112 105 179 1.11e-34 128
MsaG003098 AT3G27010.2 62.626 99 30 3 19 111 42 139 4.45e-34 123
MsaG003098 AT3G27010.1 62.626 99 30 3 19 111 42 139 4.45e-34 123
MsaG003098 AT2G45680.1 77.027 74 17 0 49 122 74 147 5.49e-34 124
MsaG003098 AT5G23280.1 65.556 90 30 1 51 140 40 128 1.50e-33 120
MsaG003098 AT5G51910.1 70.370 81 23 1 43 122 49 129 4.41e-32 118
MsaG003098 AT5G51910.2 70.370 81 23 1 43 122 49 129 4.41e-32 118
MsaG003098 AT1G35560.1 78.462 65 14 0 48 112 53 117 8.67e-32 118
MsaG003098 AT5G41030.1 59.302 86 32 1 47 132 65 147 7.58e-31 113
MsaG003098 AT3G45150.1 45.455 99 50 2 48 142 15 113 3.31e-22 89.0
MsaG003098 AT1G58100.2 88.571 35 4 0 48 82 58 92 2.74e-15 73.6

Find 87 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTGGTGTGACGGTCTT+TGG 0.326298 1:+51793773 None:intergenic
TGGAAGGTTTGGTTTCTGAT+TGG 0.336354 1:+51793880 None:intergenic
CGACTGTTGGTCCCGGCGGT+GGG 0.370781 1:-51793512 MsaT003098.1:CDS
CCTGTCTCAGCCGGCGGGGA+TGG 0.373484 1:-51793436 MsaT003098.1:CDS
ACGACTGTTGGTCCCGGCGG+TGG 0.375769 1:-51793513 MsaT003098.1:CDS
GGAAGGTTTGGTTTCTGATT+GGG 0.382038 1:+51793881 None:intergenic
ACTGTTGGTCCCGGCGGTGG+GGG 0.398278 1:-51793510 MsaT003098.1:CDS
CGGTCCGGCGGGGACAGTTC+CGG 0.401737 1:+51793602 None:intergenic
TGTTACGGCGGTGGAAGGTT+TGG 0.414434 1:+51793869 None:intergenic
TCGGACGGTGAGTTTGGTGT+TGG 0.415353 1:+51793818 None:intergenic
CGGACGGTGAGTTTGGTGTT+GGG 0.422384 1:+51793819 None:intergenic
AGGTCTTCGGACGGTGAGTT+TGG 0.426516 1:+51793812 None:intergenic
CAGCCGGCGGGGATGGATTA+CGG 0.432604 1:-51793429 MsaT003098.1:CDS
GGTGGCGACGGTGGTTGTTA+CGG 0.437898 1:+51793854 None:intergenic
GACTGTTGGTCCCGGCGGTG+GGG 0.441550 1:-51793511 MsaT003098.1:CDS
TTTGGTGTTGGGATCGGAAG+AGG 0.447134 1:+51793830 None:intergenic
GACCGTCACACCAAGGTTAA+CGG 0.448472 1:-51793768 MsaT003098.1:CDS
GCATACGGACACGGCGGCCA+CGG 0.450952 1:+51793745 None:intergenic
GTTGGGATCGGAAGAGGTGG+TGG 0.463601 1:+51793836 None:intergenic
CGGCAGCTCGGCTGTTGCAT+CGG 0.466946 1:+51793465 None:intergenic
CCATCCCCGCCGGCTGAGAC+AGG 0.467844 1:+51793436 None:intergenic
CCGTCGATCATCGCCGCTAC+AGG 0.469019 1:-51793627 MsaT003098.1:CDS
ATCGAGAGGTGGAAGGTCTT+CGG 0.469473 1:+51793799 None:intergenic
CTTGACCTGTCTCAGCCGGC+GGG 0.470366 1:-51793441 MsaT003098.1:CDS
TTCTCCGTTTAAGTCTTCCT+CGG 0.470391 1:-51793568 MsaT003098.1:CDS
CGGAACTGTCCCCGCCGGAC+CGG 0.473172 1:-51793601 MsaT003098.1:CDS
AACGGAGAAGAGGTGGAAAC+CGG 0.477385 1:+51793582 None:intergenic
GAGGTGGAAACCGGTCCGGC+GGG 0.478968 1:+51793591 None:intergenic
CGGCGCAGAGTGGCGGCATA+CGG 0.487397 1:+51793730 None:intergenic
GGCCGTTAACCTTGGTGTGA+CGG 0.487689 1:+51793766 None:intergenic
GACCTAGCTCACGTGTGAGC+TGG 0.488472 1:+51793697 None:intergenic
TGGATTACGGCACGGCGAGC+AGG 0.491585 1:-51793416 MsaT003098.1:CDS
ATCGGAAGAGGTGGTGGCGA+CGG 0.493308 1:+51793842 None:intergenic
CACACCAAGGTTAACGGCCG+TGG 0.500084 1:-51793762 MsaT003098.1:CDS
GGTGAGTTTGGTGTTGGGAT+CGG 0.500790 1:+51793824 None:intergenic
CCTGTGACGACTGTTGGTCC+CGG 0.512876 1:-51793519 MsaT003098.1:CDS
GGTGTTGGGATCGGAAGAGG+TGG 0.520667 1:+51793833 None:intergenic
CTGTTGGTCCCGGCGGTGGG+GGG 0.522672 1:-51793509 MsaT003098.1:CDS
AGAAGAGGTGGAAACCGGTC+CGG 0.523967 1:+51793587 None:intergenic
ATCATCGCCGCTACAGGTAC+CGG 0.527492 1:-51793621 MsaT003098.1:CDS
GTGGTTGTTACGGCGGTGGA+AGG 0.530957 1:+51793864 None:intergenic
CTTGGTGTGACGGTCTTTGG+TGG 0.533419 1:+51793776 None:intergenic
GCGGCCACGGCCGTTAACCT+TGG 0.533810 1:+51793758 None:intergenic
TAAGTCTTCCTCGGTGCCGT+CGG 0.539714 1:-51793559 MsaT003098.1:CDS
CCGTCTTGACCTGTCTCAGC+CGG 0.539844 1:-51793445 MsaT003098.1:CDS
AGAGGTGGAAACCGGTCCGG+CGG 0.541236 1:+51793590 None:intergenic
GACGGTGGTTGTTACGGCGG+TGG 0.541430 1:+51793860 None:intergenic
TTCCAGCTCACACGTGAGCT+AGG 0.544068 1:-51793699 MsaT003098.1:CDS
GGCACCGAGGAAGACTTAAA+CGG 0.544702 1:+51793564 None:intergenic
CAGCTCGGCTGTTGCATCGG+CGG 0.554896 1:+51793468 None:intergenic
ATTCTAGCGGCGCAGAGTGG+CGG 0.558629 1:+51793723 None:intergenic
CCGGGACCAACAGTCGTCAC+AGG 0.561674 1:+51793519 None:intergenic
TCTTGACCTGTCTCAGCCGG+CGG 0.565908 1:-51793442 MsaT003098.1:CDS
GGAAGAGGTGGTGGCGACGG+TGG 0.571531 1:+51793845 None:intergenic
GACAGTTCCGGTACCTGTAG+CGG 0.580616 1:+51793614 None:intergenic
CCTGTAGCGGCGATGATCGA+CGG 0.581417 1:+51793627 None:intergenic
GTCTTTGGTGGATCGAGAGG+TGG 0.588804 1:+51793788 None:intergenic
TGCAAAAGCAGCGACGTGAA+CGG 0.596315 1:+51793381 None:intergenic
AGACTTAAACGGAGAAGAGG+TGG 0.603538 1:+51793575 None:intergenic
GTGCCGTAATCCATCCCCGC+CGG 0.610684 1:+51793426 None:intergenic
GACAGGTCAAGACGGCAGCT+CGG 0.611079 1:+51793453 None:intergenic
TACAACAATGTCTTCATCAG+AGG 0.612302 1:-51793928 None:intergenic
CGATCGTTTCACCGTCGGAA+CGG 0.615737 1:+51793673 None:intergenic
GGAAGACTTAAACGGAGAAG+AGG 0.618830 1:+51793572 None:intergenic
GTTCAGCCTGTGACGACTGT+TGG 0.621754 1:-51793525 MsaT003098.1:CDS
CCGGCTGAGACAGGTCAAGA+CGG 0.630042 1:+51793445 None:intergenic
AGAGTGGCGGCATACGGACA+CGG 0.630769 1:+51793736 None:intergenic
ATTGGGTTTGAGAGAATGTG+TGG 0.635459 1:+51793898 None:intergenic
AGGTGGAAACCGGTCCGGCG+GGG 0.639096 1:+51793592 None:intergenic
GGTACCGGAACTGTCCCCGC+CGG 0.641499 1:-51793606 MsaT003098.1:CDS
TTGGTGGATCGAGAGGTGGA+AGG 0.641652 1:+51793792 None:intergenic
GGCGACGGTGGTTGTTACGG+CGG 0.645530 1:+51793857 None:intergenic
CCACTCGATCGTTTCACCGT+CGG 0.646740 1:+51793668 None:intergenic
GAGCTAGGTCACCGTTCCGA+CGG 0.649168 1:-51793684 MsaT003098.1:CDS
ACGGTCTTTGGTGGATCGAG+AGG 0.651900 1:+51793785 None:intergenic
GGCGGGGATGGATTACGGCA+CGG 0.663996 1:-51793424 MsaT003098.1:CDS
CACCAAAGACCGTCACACCA+AGG 0.680883 1:-51793775 MsaT003098.1:CDS
ACAAGAAACCGACGGCACCG+AGG 0.684600 1:+51793551 None:intergenic
AGAGGTGGAAGGTCTTCGGA+CGG 0.686301 1:+51793803 None:intergenic
TTGACCTGTCTCAGCCGGCG+GGG 0.687922 1:-51793440 MsaT003098.1:CDS
TGAACATGACAAGAAACCGA+CGG 0.690502 1:+51793543 None:intergenic
AAGATTCTAGCGGCGCAGAG+TGG 0.698200 1:+51793720 None:intergenic
CCGACGGTGAAACGATCGAG+TGG 0.701087 1:-51793668 MsaT003098.1:CDS
TGTGAGCTGGAAGATTCTAG+CGG 0.731020 1:+51793710 None:intergenic
TTTGCAACCGTCGACCACGG+GGG 0.740183 1:-51793364 MsaT003098.1:CDS
GTGACGACTGTTGGTCCCGG+CGG 0.740863 1:-51793516 MsaT003098.1:CDS
GTGGCGGCATACGGACACGG+CGG 0.797562 1:+51793739 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 51793323 51793943 51793323 ID=MsaG003098
Chr1 mRNA 51793323 51793943 51793323 ID=MsaT003098.1;Parent=MsaG003098
Chr1 exon 51793323 51793943 51793323 ID=MsaT003098.1.exon1;Parent=MsaT003098.1
Chr1 CDS 51793323 51793943 51793323 ID=cds.MsaT003098.1;Parent=MsaT003098.1
Gene Sequence

>MsaT003098.1

ATGTCTTCATCAGAGGTAGTTATCCACACATTCTCTCAAACCCAATCAGAAACCAAACCTTCCACCGCCGTAACAACCACCGTCGCCACCACCTCTTCCGATCCCAACACCAAACTCACCGTCCGAAGACCTTCCACCTCTCGATCCACCAAAGACCGTCACACCAAGGTTAACGGCCGTGGCCGCCGTGTCCGTATGCCGCCACTCTGCGCCGCTAGAATCTTCCAGCTCACACGTGAGCTAGGTCACCGTTCCGACGGTGAAACGATCGAGTGGCTTCTTCGTCATGCTGAGCCGTCGATCATCGCCGCTACAGGTACCGGAACTGTCCCCGCCGGACCGGTTTCCACCTCTTCTCCGTTTAAGTCTTCCTCGGTGCCGTCGGTTTCTTGTCATGTTCAGCCTGTGACGACTGTTGGTCCCGGCGGTGGGGGGATTTTCGGTATGGCTTTTCCGCCGATGCAACAGCCGAGCTGCCGTCTTGACCTGTCTCAGCCGGCGGGGATGGATTACGGCACGGCGAGCAGGTATCGTCATATGCCGTTCACGTCGCTGCTTTTGCAACCGTCGACCACGGGGGAGGAGAATCAACAAGAAGAAGAAGCTCTTAACGAACAATGA

Protein sequence

>MsaT003098.1

MSSSEVVIHTFSQTQSETKPSTAVTTTVATTSSDPNTKLTVRRPSTSRSTKDRHTKVNGRGRRVRMPPLCAARIFQLTRELGHRSDGETIEWLLRHAEPSIIAATGTGTVPAGPVSTSSPFKSSSVPSVSCHVQPVTTVGPGGGGIFGMAFPPMQQPSCRLDLSQPAGMDYGTASRYRHMPFTSLLLQPSTTGEENQQEEEALNEQ*