AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003163


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003163 MtrunA17_Chr1g0182181 95.122 164 8 0 5 168 2 165 3.62e-114 320
MsaG003163 MtrunA17_Chr7g0263121 64.327 171 53 4 1 165 1 169 5.51e-71 211
MsaG003163 MtrunA17_Chr1g0182161 63.429 175 47 8 1 166 2 168 8.18e-63 191
MsaG003163 MtrunA17_Chr7g0263131 58.896 163 57 5 1 162 3 156 9.55e-58 178
MsaG003163 MtrunA17_Chr2g0327901 49.677 155 49 6 1 153 1 128 1.10e-35 120
MsaG003163 MtrunA17_Chr1g0212561 41.262 206 77 8 1 167 1 201 2.00e-33 117
MsaG003163 MtrunA17_Chr7g0222831 52.101 119 49 3 1 115 1 115 4.67e-32 113
MsaG003163 MtrunA17_Chr2g0320311 32.314 229 61 7 7 154 68 283 3.09e-22 90.5
MsaG003163 MtrunA17_Chr1g0197281 34.826 201 65 7 10 146 98 296 2.46e-21 89.0
MsaG003163 MtrunA17_Chr1g0180311 38.095 147 45 3 13 113 80 226 1.84e-19 82.8
MsaG003163 MtrunA17_Chr1g0207451 41.584 101 54 2 14 112 60 157 3.19e-17 76.3
MsaG003163 MtrunA17_Chr4g0012691 46.429 84 35 2 38 111 220 303 4.55e-16 74.3
MsaG003163 MtrunA17_Chr4g0027991 31.707 164 56 3 38 154 113 267 6.44e-16 73.2
MsaG003163 MtrunA17_Chr7g0231181 38.983 118 54 6 38 152 240 342 1.87e-15 72.4
MsaG003163 MtrunA17_Chr3g0134711 36.620 142 66 5 2 119 37 178 6.99e-14 67.0
MsaG003163 MtrunA17_Chr1g0152661 36.066 122 58 4 36 146 79 191 2.72e-13 65.5
MsaG003163 MtrunA17_Chr1g0188911 34.783 115 34 3 38 112 44 157 7.98e-13 63.5
MsaG003163 MtrunA17_Chr1g0188901 40.000 95 42 3 38 117 60 154 1.10e-12 62.8
MsaG003163 MtrunA17_Chr4g0061301 33.333 126 45 3 38 124 348 473 7.75e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003163 AT2G37430.1 45.251 179 84 6 1 168 1 176 1.10e-42 140
MsaG003163 AT3G53600.1 43.956 182 79 7 1 168 1 173 1.04e-36 125
MsaG003163 AT2G28710.1 46.061 165 69 7 1 158 1 152 9.93e-33 114
MsaG003163 AT5G59820.1 45.455 154 71 5 11 158 12 158 5.73e-29 105
MsaG003163 AT2G28200.1 35.714 196 73 7 10 154 73 266 2.16e-26 101
MsaG003163 AT3G46090.1 43.293 164 67 6 9 158 11 162 2.45e-26 98.6
MsaG003163 AT3G46080.1 43.902 164 75 7 5 158 4 160 7.61e-26 97.1
MsaG003163 AT3G46070.1 40.491 163 79 7 1 151 1 157 1.75e-22 88.6
MsaG003163 AT5G03510.1 41.497 147 39 6 10 109 69 215 1.02e-21 89.4
MsaG003163 AT5G04390.1 36.471 170 47 5 1 112 89 255 1.31e-20 87.4
MsaG003163 AT3G10470.1 44.660 103 26 2 38 109 182 284 1.91e-18 81.6
MsaG003163 AT2G45120.1 36.196 163 74 5 13 152 150 305 1.02e-17 79.0
MsaG003163 AT3G60580.1 32.530 166 76 4 13 152 126 281 1.49e-16 75.5
MsaG003163 AT1G02030.1 48.718 78 39 1 38 114 160 237 1.04e-15 72.8
MsaG003163 AT1G27730.1 33.113 151 87 4 9 149 50 196 3.03e-15 70.9
MsaG003163 AT1G49900.1 36.667 120 70 3 25 140 735 852 9.17e-15 71.6
MsaG003163 AT1G49900.1 33.582 134 85 2 38 167 193 326 3.32e-13 67.0
MsaG003163 AT3G49930.1 37.931 116 47 5 14 108 60 171 4.25e-14 67.8
MsaG003163 AT5G43170.1 34.247 146 77 5 9 151 45 174 3.66e-13 64.7
MsaG003163 AT5G56200.1 44.444 90 29 2 40 108 344 433 6.68e-13 65.9
MsaG003163 AT4G35280.1 39.286 112 50 3 10 108 138 244 7.62e-13 65.1
MsaG003163 AT3G19580.1 34.646 127 61 5 4 108 61 187 1.38e-12 64.3
MsaG003163 AT3G19580.2 34.646 127 61 5 4 108 61 187 1.38e-12 64.3
MsaG003163 AT5G67450.1 37.363 91 38 2 37 108 96 186 1.68e-12 63.9
MsaG003163 AT5G04340.1 41.176 85 39 2 38 111 89 173 1.74e-11 60.8

Find 41 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTTGGTTTGGTTTCTT+TGG 0.133578 1:-52713481 None:intergenic
TTCTCTCTTGGCCAGGCTTT+GGG 0.201073 1:+52713693 MsaT003163.1:CDS
GAAGTTACTGTTCGGAAATA+TGG 0.265066 1:+52713869 MsaT003163.1:CDS
AAGCTACATGCCCAAACTTT+AGG 0.279584 1:+52713621 MsaT003163.1:CDS
TTCTTTGGTTTGGTTTCTTT+GGG 0.287528 1:-52713480 None:intergenic
ACAGGACTAAAATGTTTCTT+TGG 0.294036 1:-52713495 None:intergenic
TATTCAGCAAACCTAAAGTT+TGG 0.298957 1:-52713632 None:intergenic
ACTAAAATGTTTCTTTGGTT+TGG 0.313841 1:-52713490 None:intergenic
GAGTTTCACGGTATCTAGTT+TGG 0.314780 1:-52713598 None:intergenic
ATTCTCTCTTGGCCAGGCTT+TGG 0.331279 1:+52713692 MsaT003163.1:CDS
TGAAAAGATCGAATAGTAAG+AGG 0.344770 1:+52713802 MsaT003163.1:CDS
AGTTTCACGGTATCTAGTTT+GGG 0.350342 1:-52713597 None:intergenic
AATGTAGCACCCATGCGTTT+TGG 0.353287 1:-52713658 None:intergenic
ATTCAGCAAACCTAAAGTTT+GGG 0.384341 1:-52713631 None:intergenic
AAGAGTGGAGAATATAGATT+TGG 0.395790 1:+52713431 MsaT003163.1:CDS
CATATGAGAAGGCACAAAAT+TGG 0.403275 1:+52713720 MsaT003163.1:CDS
TGTGGTAAAGAATTCTCTCT+TGG 0.452482 1:+52713681 MsaT003163.1:CDS
CTGAATAACCCCAAAACGCA+TGG 0.471935 1:+52713648 MsaT003163.1:CDS
TTAATTTCGTAGCCATGAAA+AGG 0.477789 1:+52713397 None:intergenic
ACGCATGGGTGCTACATTTG+TGG 0.490183 1:+52713663 MsaT003163.1:CDS
GGCATGTAGCTTGAGTTTCA+CGG 0.491665 1:-52713610 None:intergenic
TCATATGTCCTCCCAAAGCC+TGG 0.497850 1:-52713704 None:intergenic
TAGTAAGAGGGTTATGTGCT+TGG 0.501643 1:+52713815 MsaT003163.1:CDS
TAAAGAATTCTCTCTTGGCC+AGG 0.519657 1:+52713686 MsaT003163.1:CDS
TTCTCTTCGTTTCAAGCCCT+AGG 0.530440 1:+52713549 MsaT003163.1:CDS
AATGACTTGAAGTTACTGTT+CGG 0.533779 1:+52713861 MsaT003163.1:CDS
GAAAAGATCGAATAGTAAGA+GGG 0.539509 1:+52713803 MsaT003163.1:CDS
TCTCTTGGCCAGGCTTTGGG+AGG 0.541746 1:+52713696 MsaT003163.1:CDS
GACTAGCTCTATGGCCACCT+AGG 0.545631 1:-52713566 None:intergenic
CACAAAATTGGCGTCAACGA+GGG 0.547186 1:+52713732 MsaT003163.1:CDS
TTTCTTTGGGATGAGAGAGT+AGG 0.548800 1:-52713467 None:intergenic
AAGGCAGAGAGACGAAAGAG+TGG 0.561042 1:+52713416 MsaT003163.1:CDS
ACTAGCTCTATGGCCACCTA+GGG 0.585030 1:-52713565 None:intergenic
TGAATAACCCCAAAACGCAT+GGG 0.610752 1:+52713649 MsaT003163.1:CDS
AACTTCAAGTCATTCTGCAA+AGG 0.613686 1:-52713852 None:intergenic
GTGGTGCACTCAAATTCCAC+AGG 0.615607 1:-52713513 None:intergenic
CTTTGGGAGGACATATGAGA+AGG 0.632803 1:+52713709 MsaT003163.1:CDS
TCTTCGTTTCAAGCCCTAGG+TGG 0.635082 1:+52713552 MsaT003163.1:CDS
CTTGAAACGAAGAGAACTTG+CGG 0.655759 1:-52713542 None:intergenic
AGAGAACTTGCGGTTGCATG+TGG 0.663988 1:-52713532 None:intergenic
GCACAAAATTGGCGTCAACG+AGG 0.669929 1:+52713731 MsaT003163.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 52713411 52713917 52713411 ID=MsaG003163
Chr1 mRNA 52713411 52713917 52713411 ID=MsaT003163.1;Parent=MsaG003163
Chr1 exon 52713411 52713917 52713411 ID=MsaT003163.1.exon1;Parent=MsaT003163.1
Chr1 CDS 52713411 52713917 52713411 ID=cds.MsaT003163.1;Parent=MsaT003163.1
Gene Sequence

>MsaT003163.1

ATGAAAAGGCAGAGAGACGAAAGAGTGGAGAATATAGATTTGGCAAATTGTCTAATCCTACTCTCTCATCCCAAAGAAACCAAACCAAAGAAACATTTTAGTCCTGTGGAATTTGAGTGCACCACATGCAACCGCAAGTTCTCTTCGTTTCAAGCCCTAGGTGGCCATAGAGCTAGTCACAAAAAGCCCAAACTAGATACCGTGAAACTCAAGCTACATGCCCAAACTTTAGGTTTGCTGAATAACCCCAAAACGCATGGGTGCTACATTTGTGGTAAAGAATTCTCTCTTGGCCAGGCTTTGGGAGGACATATGAGAAGGCACAAAATTGGCGTCAACGAGGGATTTTCTTTGGTGAAGAAAAATAAGGTTGTTGGAAAAATCCCAATATTGAAAAGATCGAATAGTAAGAGGGTTATGTGCTTGGACTTGAACTTAACACCTTTGCAGAATGACTTGAAGTTACTGTTCGGAAATATGGCACCTGAAGTTGATTCATTTTTTTGA

Protein sequence

>MsaT003163.1

MKRQRDERVENIDLANCLILLSHPKETKPKKHFSPVEFECTTCNRKFSSFQALGGHRASHKKPKLDTVKLKLHAQTLGLLNNPKTHGCYICGKEFSLGQALGGHMRRHKIGVNEGFSLVKKNKVVGKIPILKRSNSKRVMCLDLNLTPLQNDLKLLFGNMAPEVDSFF*