Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003193 | GAU26649.1 | 84.706 | 85 | 13 | 0 | 1 | 85 | 1 | 85 | 4.22e-43 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003193 | sp|Q9LXT3|MBF1B_ARATH | 65.882 | 85 | 29 | 0 | 1 | 85 | 1 | 85 | 8.50e-36 | 121 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003193 | tr|A0A2Z6N563|A0A2Z6N563_TRISU | 84.706 | 85 | 13 | 0 | 1 | 85 | 1 | 85 | 2.01e-43 | 147 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG003193 | TR | MBF1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003193 | MtrunA17_Chr2g0320211 | 86.420 | 81 | 11 | 0 | 5 | 85 | 3 | 83 | 1.70e-44 | 140 |
| MsaG003193 | MtrunA17_Chr4g0041301 | 77.647 | 85 | 19 | 0 | 1 | 85 | 1 | 85 | 3.84e-36 | 119 |
| MsaG003193 | MtrunA17_Chr4g0057871 | 81.579 | 38 | 7 | 0 | 32 | 69 | 9 | 46 | 9.34e-14 | 61.6 |
| MsaG003193 | MtrunA17_Chr6g0485281 | 42.424 | 66 | 38 | 0 | 4 | 69 | 9 | 74 | 2.35e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003193 | AT3G58680.1 | 65.882 | 85 | 29 | 0 | 1 | 85 | 1 | 85 | 8.64e-37 | 121 |
| MsaG003193 | AT2G42680.1 | 65.882 | 85 | 29 | 0 | 1 | 85 | 1 | 85 | 5.62e-35 | 116 |
Find 24 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTTCATGGTTTCAATAT+CGG | 0.263054 | 1:-53263242 | None:intergenic |
| CAAAGAATTTAGCTCATGTT+TGG | 0.307412 | 1:+53263335 | MsaT003193.1:CDS |
| CCCAACTGAACTCAAGAAAA+AGG | 0.324733 | 1:+53263360 | MsaT003193.1:CDS |
| CGCAGCATAATGTTTCTTCA+TGG | 0.338481 | 1:-53263254 | None:intergenic |
| TCGGGCCTGTTTCACAAGAT+TGG | 0.356885 | 1:+53263134 | MsaT003193.1:CDS |
| GGTTCCCAATCTTGTGAAAC+AGG | 0.382368 | 1:-53263139 | None:intergenic |
| AAAGAATTTAGCTCATGTTT+GGG | 0.398502 | 1:+53263336 | MsaT003193.1:CDS |
| CGGGCCTGTTTCACAAGATT+GGG | 0.408200 | 1:+53263135 | MsaT003193.1:CDS |
| CATTCAACCATGTCAGGAAT+CGG | 0.422042 | 1:+53263115 | None:intergenic |
| GAGCTAAATTCTTTGTATCC+TGG | 0.425930 | 1:-53263327 | None:intergenic |
| GCACTTCATTAAACACTAAG+AGG | 0.463082 | 1:+53263299 | MsaT003193.1:CDS |
| TCAAATCATTCAACCATGTC+AGG | 0.463950 | 1:+53263109 | None:intergenic |
| GCTTTCTTGCGGAGAACGAC+GGG | 0.490830 | 1:-53263160 | None:intergenic |
| GTCAACGCCACTCGCCGTGC+CGG | 0.496886 | 1:+53263217 | MsaT003193.1:CDS |
| ATTCAACCATGTCAGGAATC+GGG | 0.510589 | 1:+53263116 | None:intergenic |
| CATGGTTTCAATATCGGCAC+CGG | 0.541173 | 1:-53263236 | None:intergenic |
| ATCGGCACCGGCACGGCGAG+TGG | 0.547151 | 1:-53263224 | None:intergenic |
| AGCTTTCTTGCGGAGAACGA+CGG | 0.554482 | 1:-53263161 | None:intergenic |
| AACAGGCCCGATTCCTGACA+TGG | 0.561106 | 1:-53263122 | None:intergenic |
| AAGCGATGGGAGCTTTCTTG+CGG | 0.583947 | 1:-53263171 | None:intergenic |
| AAACACTAAGAGGCTAAACC+AGG | 0.598430 | 1:+53263309 | MsaT003193.1:CDS |
| TTTCAATATCGGCACCGGCA+CGG | 0.609948 | 1:-53263231 | None:intergenic |
| TAATGAAGTGCTGCTAGAGG+CGG | 0.621547 | 1:-53263287 | None:intergenic |
| GTTTAATGAAGTGCTGCTAG+AGG | 0.639627 | 1:-53263290 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 53263124 | 53263399 | 53263124 | ID=MsaG003193 |
| Chr1 | mRNA | 53263124 | 53263399 | 53263124 | ID=MsaT003193.1;Parent=MsaG003193 |
| Chr1 | exon | 53263124 | 53263399 | 53263124 | ID=MsaT003193.1.exon1;Parent=MsaT003193.1 |
| Chr1 | CDS | 53263124 | 53263399 | 53263124 | ID=cds.MsaT003193.1;Parent=MsaT003193.1 |
| Gene Sequence |
| Protein sequence |
