AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003223

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003223 MtrunA17_Chr1g0198761 73.117 770 191 5 1 765 1 759 0.0 1150
MsaG003223 MtrunA17_Chr5g0431001 75.052 481 110 2 1 471 1 481 0.0 745
MsaG003223 MtrunA17_Chr2g0276841 48.036 662 320 7 19 672 42 687 0.0 654
MsaG003223 MtrunA17_Chr2g0294841 42.507 734 381 10 34 739 25 745 0.0 600
MsaG003223 MtrunA17_Chr2g0290391 46.772 635 299 9 53 666 50 666 0.0 564
MsaG003223 MtrunA17_Chr1g0202341 30.795 604 395 10 19 614 20 608 2.02e-86 288
MsaG003223 MtrunA17_Chr5g0448761 31.552 580 363 13 54 614 60 624 1.59e-81 277
MsaG003223 MtrunA17_Chr3g0080351 29.202 589 351 14 54 616 76 624 3.78e-76 263
MsaG003223 MtrunA17_Chr2g0296781 29.472 682 429 20 60 716 3 657 1.23e-74 257
MsaG003223 MtrunA17_Chr5g0441581 29.635 685 421 22 52 711 30 678 8.96e-72 248
MsaG003223 MtrunA17_Chr1g0203051 27.636 550 368 10 66 603 91 622 2.35e-69 241
MsaG003223 MtrunA17_Chr2g0329031 27.221 709 439 20 60 733 20 686 4.26e-69 242
MsaG003223 MtrunA17_Chr6g0458391 29.361 579 372 9 46 616 217 766 8.31e-69 242
MsaG003223 MtrunA17_Chr1g0207551 30.261 575 371 12 54 616 34 590 1.15e-68 242
MsaG003223 MtrunA17_Chr2g0287651 28.722 571 371 13 60 614 81 631 1.98e-66 237
MsaG003223 MtrunA17_Chr2g0287061 28.547 578 336 15 54 614 183 700 2.72e-66 235
MsaG003223 MtrunA17_Chr8g0363841 28.348 575 381 12 55 614 18 576 9.47e-66 231
MsaG003223 MtrunA17_Chr6g0477261 31.397 551 340 16 184 710 4 540 2.03e-65 230
MsaG003223 MtrunA17_Chr5g0440451 26.170 684 425 17 54 697 43 686 4.36e-64 231
MsaG003223 MtrunA17_Chr3g0082121 29.479 614 374 23 43 636 32 606 9.80e-63 223
MsaG003223 MtrunA17_Chr2g0324011 27.491 582 393 14 46 614 2 567 1.44e-59 212
MsaG003223 MtrunA17_Chr1g0196541 27.310 725 451 22 60 750 3 685 6.18e-58 211
MsaG003223 MtrunA17_Chr1g0203721 26.170 577 391 11 57 616 66 624 6.26e-57 209
MsaG003223 MtrunA17_Chr5g0448511 32.011 378 250 3 239 610 16 392 1.23e-55 200
MsaG003223 MtrunA17_Chr3g0118611 30.189 424 284 6 193 610 79 496 1.13e-54 202
MsaG003223 MtrunA17_Chr2g0291581 23.449 661 424 14 53 674 70 687 2.19e-49 185
MsaG003223 MtrunA17_Chr3g0081281 87.097 93 12 0 199 291 1 93 2.52e-48 165
MsaG003223 MtrunA17_Chr5g0430921 79.545 88 15 1 669 756 1 85 4.31e-41 145
MsaG003223 MtrunA17_Chr2g0291591 24.953 533 347 14 54 563 64 566 2.72e-40 157
MsaG003223 MtrunA17_Chr1g0164591 32.524 206 137 2 250 454 104 308 3.75e-34 133
MsaG003223 MtrunA17_Chr7g0258861 25.253 297 207 6 46 337 2 288 2.30e-23 101
MsaG003223 MtrunA17_Chr2g0308841 26.126 222 154 5 408 623 14 231 2.25e-17 83.2
MsaG003223 MtrunA17_Chr1g0171741 32.203 118 68 3 60 174 139 247 6.18e-14 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003223 AT1G10240.2 49.206 630 309 4 48 672 42 665 0.0 642
MsaG003223 AT1G10240.1 49.206 630 309 4 48 672 42 665 0.0 642
MsaG003223 AT5G28530.3 43.407 675 338 11 25 678 28 679 0.0 547
MsaG003223 AT5G28530.2 43.407 675 338 11 25 678 28 679 0.0 547
MsaG003223 AT5G28530.1 43.407 675 342 11 25 678 28 683 0.0 547
MsaG003223 AT5G28530.4 38.797 665 324 12 25 678 28 620 3.99e-148 448
MsaG003223 AT5G28530.5 38.797 665 320 12 25 678 28 616 4.01e-148 448
MsaG003223 AT4G38180.1 30.743 592 386 10 38 617 59 638 6.35e-84 284
MsaG003223 AT4G15090.1 28.716 693 443 16 54 721 51 717 2.66e-81 278
MsaG003223 AT4G15090.5 28.716 693 443 16 54 721 51 717 2.66e-81 278
MsaG003223 AT4G15090.3 28.716 693 443 16 54 721 51 717 2.66e-81 278
MsaG003223 AT4G15090.2 28.716 693 443 16 54 721 51 717 2.66e-81 278
MsaG003223 AT4G15090.4 28.716 693 443 16 54 721 51 717 2.66e-81 278
MsaG003223 AT1G76320.3 28.850 565 360 11 60 610 3 539 3.89e-67 236
MsaG003223 AT1G76320.2 28.850 565 360 11 60 610 3 539 8.85e-67 236
MsaG003223 AT1G76320.1 28.850 565 360 11 60 610 3 539 9.52e-67 236
MsaG003223 AT1G76320.4 28.850 565 360 11 60 610 3 539 9.52e-67 236
MsaG003223 AT3G22170.2 25.547 685 429 20 54 705 70 706 7.00e-66 235
MsaG003223 AT3G22170.1 25.547 685 429 20 54 705 70 706 7.00e-66 235
MsaG003223 AT5G18960.2 26.014 592 364 13 39 616 198 729 5.33e-59 215
MsaG003223 AT5G18960.1 26.014 592 364 13 39 616 198 729 5.33e-59 215
MsaG003223 AT3G06250.2 25.997 577 352 12 54 616 190 705 3.09e-58 212
MsaG003223 AT3G06250.1 25.997 577 352 12 54 616 190 705 3.09e-58 212
MsaG003223 AT3G06250.3 25.997 577 352 12 54 616 190 705 3.09e-58 212
MsaG003223 AT2G27110.2 25.896 614 403 13 54 658 49 619 5.36e-55 204
MsaG003223 AT2G27110.1 25.896 614 403 13 54 658 49 619 5.36e-55 204
MsaG003223 AT2G27110.3 28.637 433 291 9 235 658 51 474 1.03e-50 190
MsaG003223 AT2G32250.5 24.518 571 361 11 57 615 41 553 6.03e-49 185
MsaG003223 AT4G19990.5 26.451 586 354 14 56 614 23 558 7.35e-49 185
MsaG003223 AT4G19990.6 26.451 586 354 14 56 614 23 558 8.32e-49 185
MsaG003223 AT4G19990.2 26.451 586 354 14 56 614 23 558 8.32e-49 185
MsaG003223 AT4G19990.3 26.451 586 354 14 56 614 23 558 9.04e-49 185
MsaG003223 AT4G19990.4 26.451 586 354 14 56 614 23 558 9.04e-49 185
MsaG003223 AT4G19990.7 26.451 586 354 14 56 614 37 572 1.12e-48 184
MsaG003223 AT2G32250.2 24.518 571 361 11 57 615 41 553 1.21e-48 185
MsaG003223 AT2G32250.4 24.518 571 361 11 57 615 41 553 1.21e-48 185
MsaG003223 AT2G32250.6 24.518 571 361 11 57 615 41 553 1.25e-48 185
MsaG003223 AT2G32250.1 24.518 571 361 11 57 615 41 553 1.25e-48 185
MsaG003223 AT2G32250.3 24.518 571 361 11 57 615 41 553 1.25e-48 185
MsaG003223 AT1G52520.1 25.685 584 380 17 53 613 84 636 1.67e-48 183
MsaG003223 AT2G32250.8 24.472 568 359 11 60 615 3 512 1.69e-48 184
MsaG003223 AT2G32250.7 24.472 568 359 11 60 615 3 512 2.05e-48 184
MsaG003223 AT4G38170.1 27.027 481 318 10 267 726 45 513 6.55e-46 173
MsaG003223 AT1G80010.1 24.876 603 372 18 54 613 95 659 8.36e-46 176
MsaG003223 AT4G19990.1 26.248 541 337 12 86 614 11 501 1.58e-44 171
MsaG003223 AT3G59470.3 37.273 110 61 3 54 163 70 171 2.12e-13 71.2
MsaG003223 AT3G59470.1 37.273 110 61 3 54 163 70 171 2.12e-13 71.2
MsaG003223 AT3G59470.2 37.273 110 61 3 54 163 82 183 2.79e-13 71.2

Find 113 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGATAATTTGATTTATT+TGG 0.093166 1:+53623925 MsaT003223.1:CDS
TCTACTCTCATCGTCAATTT+TGG 0.121901 1:+53623122 MsaT003223.1:CDS
TACAGGTAACTTACCATTTC+TGG 0.139830 1:+53622350 MsaT003223.1:CDS
TTGACTATATGACTGCATTT+TGG 0.141368 1:-53623761 None:intergenic
ACATCATAACCATCATTATT+TGG 0.153794 1:-53623968 None:intergenic
CTAAAGGCTTTGAAGGAAAA+TGG 0.187093 1:+53622148 MsaT003223.1:CDS
TTCATTCAGATGGGCTTTAA+AGG 0.197395 1:+53622704 MsaT003223.1:CDS
ACAAACTAGAGCATATTATT+TGG 0.199763 1:+53622511 MsaT003223.1:CDS
ACAATCAGAACTTACTTATT+AGG 0.237831 1:-53624169 None:intergenic
TGAACGAGAATGGGAATTAA+TGG 0.241089 1:+53623057 MsaT003223.1:CDS
ATCAAATTATCTTGACTTGC+AGG 0.274591 1:-53623917 None:intergenic
GAGCATATTATTTGGGCATT+TGG 0.276151 1:+53622519 MsaT003223.1:CDS
ATTCGAGCATATGAATCTTT+TGG 0.301566 1:+53622549 MsaT003223.1:CDS
TTGTCCTAATTAGAGAATTA+TGG 0.303893 1:-53623418 None:intergenic
AGTACCATAATTCTCTAATT+AGG 0.305229 1:+53623414 MsaT003223.1:CDS
TGATTGGTTCCGCCGCAATT+TGG 0.306685 1:-53624088 None:intergenic
GTGACAACGTCGAAGCTTCT+TGG 0.321956 1:-53624062 None:intergenic
AATCATCTATAGATTCTAAA+TGG 0.332504 1:-53623033 None:intergenic
GAATTAATGGTTGCACAATT+TGG 0.333830 1:+53623070 MsaT003223.1:CDS
TTTGCAGCATGTTCCTCAAT+AGG 0.334456 1:-53623446 None:intergenic
GCAGCTTCAACTCCACCTTT+TGG 0.341287 1:-53624040 None:intergenic
AAATTCCTCCGACAGATAAA+AGG 0.354413 1:+53623890 MsaT003223.1:CDS
CATATAGTCAAATGCAATAA+TGG 0.354919 1:+53623773 MsaT003223.1:CDS
ACTTTAAACAGGAGTTTCAT+AGG 0.356852 1:+53623005 MsaT003223.1:CDS
CTAATATCCTTCTCCAGAAA+TGG 0.365717 1:-53622363 None:intergenic
TGCCACTTGGAATGTGGATT+GGG 0.366378 1:+53622619 MsaT003223.1:CDS
CAAACTAGAGCATATTATTT+GGG 0.370107 1:+53622512 MsaT003223.1:CDS
TCAGTCTCTAATTCGATGTT+TGG 0.382120 1:+53623811 MsaT003223.1:CDS
TTGGTTTCTAAAGGCTTTGA+AGG 0.384106 1:+53622141 MsaT003223.1:CDS
TTCAACTTAAGAACAATTGT+AGG 0.397129 1:-53624321 None:intergenic
GAGATATATTGACTTTAAAC+AGG 0.399521 1:+53622994 MsaT003223.1:CDS
TTTGCTGGGATGACGACAAC+TGG 0.403764 1:+53623172 MsaT003223.1:CDS
ATGTAAAAGTCTGAAAGATA+TGG 0.416400 1:+53622449 MsaT003223.1:CDS
TAAAGGCTTTGAAGGAAAAT+GGG 0.419353 1:+53622149 MsaT003223.1:CDS
AAGTTGAAGATAATGAATAT+TGG 0.420264 1:+53624336 MsaT003223.1:CDS
TCATGAACAGTACTAGTTCC+AGG 0.424449 1:-53621853 None:intergenic
AAATACATTGTAAAATTCAT+AGG 0.433492 1:-53621920 None:intergenic
TTTGGAACATCGGGTACATT+GGG 0.437206 1:-53623950 None:intergenic
TTGGGGCCTAGCTTATTTGA+AGG 0.438270 1:+53623141 MsaT003223.1:CDS
GCAAAGTTGTTGGTTTCTAA+AGG 0.440350 1:+53622132 MsaT003223.1:CDS
GAACTAGTACTGTTCATGAA+TGG 0.450674 1:+53621857 MsaT003223.1:CDS
CCATGAGTTATTAGATGATA+AGG 0.455510 1:+53622200 MsaT003223.1:CDS
TGGATTAACTCAAACGCATA+TGG 0.457746 1:-53623479 None:intergenic
CCATCATTATTTGGAACATC+GGG 0.457944 1:-53623959 None:intergenic
ACAATTCCGCGAGTCATGAT+TGG 0.463296 1:-53624104 None:intergenic
TTGTTGCAAAGTTGTCTAGT+TGG 0.463573 1:+53622951 MsaT003223.1:CDS
TAGACGACCACCATCAACTT+TGG 0.465253 1:-53623573 None:intergenic
TAGTTGTAGCGCAAAGTTGT+TGG 0.465803 1:+53622122 MsaT003223.1:CDS
CTTTCTCTTCTCCATCGAAA+AGG 0.467284 1:-53623728 None:intergenic
AGAAGAAATCTTCATTCAGA+TGG 0.469575 1:+53622694 MsaT003223.1:CDS
AACTAGTACTGTTCATGAAT+GGG 0.472834 1:+53621858 MsaT003223.1:CDS
AAATATGCTGCCATTCATAC+AGG 0.475969 1:+53623524 MsaT003223.1:CDS
TTAGAGAAATCAATAGATAC+AGG 0.477570 1:+53622333 MsaT003223.1:CDS
CGACATCATACCAAAGTTGA+TGG 0.478069 1:+53623563 MsaT003223.1:CDS
ATTTGGAACATCGGGTACAT+TGG 0.482649 1:-53623951 None:intergenic
TAAGAAATAGTGCTAGTGAT+TGG 0.489143 1:+53621737 MsaT003223.1:CDS
ATAATTGTTACCTGTATGAA+TGG 0.491038 1:-53623534 None:intergenic
AAATCCAAAGGTCGGCCAAA+AGG 0.496241 1:+53624010 MsaT003223.1:CDS
CCAAAAGGTTCAAGGCCAAA+AGG 0.499841 1:+53624025 MsaT003223.1:CDS
GAGTCTGAAGATAATCCATC+AGG 0.500480 1:+53622000 MsaT003223.1:CDS
ATATTATTGTTATCAAAAGC+AGG 0.503849 1:+53622276 MsaT003223.1:CDS
ATGCCACTTGGAATGTGGAT+TGG 0.511770 1:+53622618 MsaT003223.1:CDS
ACCATCATTATTTGGAACAT+CGG 0.512682 1:-53623960 None:intergenic
TGATCCGTAAGGATTGTTTG+TGG 0.512910 1:-53622857 None:intergenic
GAATTTGTTTGTCATCGAGC+TGG 0.517742 1:+53622036 MsaT003223.1:CDS
CCAGGCTGAGGTTCTTGATG+TGG 0.518896 1:-53621835 None:intergenic
TGGATTGGGGTTGATAATCA+TGG 0.518964 1:+53622633 MsaT003223.1:CDS
CCCGATGTTCCAAATAATGA+TGG 0.520874 1:+53623959 MsaT003223.1:CDS
ATTTGGCAATTCCTTTGCAA+TGG 0.524413 1:-53622900 None:intergenic
CATCTAATAACTCATGGTTG+TGG 0.528510 1:-53622194 None:intergenic
TAGACAATTCAATCTCATGT+TGG 0.528559 1:-53623499 None:intergenic
CTTTAAACAGGAGTTTCATA+GGG 0.528837 1:+53623006 MsaT003223.1:CDS
AAGGTCGGCCAAAAGGTTCA+AGG 0.530766 1:+53624017 MsaT003223.1:CDS
ACTCTCATTATGAGACTCAC+AGG 0.531933 1:-53622303 None:intergenic
TAACTTACCATTTCTGGAGA+AGG 0.534997 1:+53622356 MsaT003223.1:CDS
TTCCAAGTGGCATATCATAA+CGG 0.535191 1:-53622608 None:intergenic
AATAGTCCTTCAAATAAGCT+AGG 0.536211 1:-53623147 None:intergenic
ATGGTGGTCGTCTAGTAAGC+TGG 0.537867 1:+53623582 MsaT003223.1:CDS
TGGATAGGATCACAAAGGGT+TGG 0.538952 1:+53624256 MsaT003223.1:intron
CCACATCAAGAACCTCAGCC+TGG 0.539330 1:+53621835 MsaT003223.1:CDS
CCTTATCATCTAATAACTCA+TGG 0.545462 1:-53622200 None:intergenic
ATATCCACAAACAATCCTTA+CGG 0.547845 1:+53622853 MsaT003223.1:CDS
TGAAGATAATGAATATTGGA+TGG 0.550060 1:+53624340 MsaT003223.1:CDS
AACCGTTATGATATGCCACT+TGG 0.555362 1:+53622606 MsaT003223.1:CDS
CGTTGTCACGTTCCAAATTG+CGG 0.556435 1:+53624076 MsaT003223.1:CDS
GAAGAAATCTTCATTCAGAT+GGG 0.559436 1:+53622695 MsaT003223.1:CDS
ATTGGGATGTTACGATAAGC+GGG 0.561005 1:-53622237 None:intergenic
TAGACATCAAGCAGGAGAAG+AGG 0.565504 1:+53623376 MsaT003223.1:CDS
GTCAAAATTAGACATCAAGC+AGG 0.568777 1:+53623368 MsaT003223.1:CDS
TGCACAATTTGGTCTAAGCA+AGG 0.581324 1:+53623081 MsaT003223.1:CDS
TTGTTTCAAAATCCAAAGGT+CGG 0.583293 1:+53624002 MsaT003223.1:CDS
CCTGTTGTTTCAAAATCCAA+AGG 0.583885 1:+53623998 MsaT003223.1:CDS
AATTGGGATGTTACGATAAG+CGG 0.587373 1:-53622238 None:intergenic
TTCCTGGATAGGATCACAAA+GGG 0.596754 1:+53624252 MsaT003223.1:intron
ATCATTAAAGACAAAAGAGA+GGG 0.596933 1:+53623841 MsaT003223.1:CDS
GTGCAAGATCTTGATCCGTA+AGG 0.602894 1:-53622868 None:intergenic
ATGATATGCCACTTGGAATG+TGG 0.611489 1:+53622613 MsaT003223.1:CDS
ACAGTACTAGTTCCAGGCTG+AGG 0.619979 1:-53621847 None:intergenic
TGAGTTATTAGATGATAAGG+AGG 0.622448 1:+53622203 MsaT003223.1:CDS
AGGCTTTGAAGGAAAATGGG+TGG 0.631084 1:+53622152 MsaT003223.1:CDS
AAAGGTTCAAGGCCAAAAGG+TGG 0.631199 1:+53624028 MsaT003223.1:CDS
AATCATTAAAGACAAAAGAG+AGG 0.632966 1:+53623840 MsaT003223.1:CDS
TCAATATATCTCGAACCCAA+CGG 0.634921 1:-53622983 None:intergenic
TACTTATTAGGAGATTGAGA+AGG 0.635742 1:-53624157 None:intergenic
GCCACTTGGAATGTGGATTG+GGG 0.646236 1:+53622620 MsaT003223.1:CDS
ACCCCAATCCACATTCCAAG+TGG 0.654914 1:-53622621 None:intergenic
ACTTACAGAAGCCATTGCAA+AGG 0.657210 1:+53622889 MsaT003223.1:CDS
CATCTTCAAATGTGTGTGAG+TGG 0.665385 1:+53624286 MsaT003223.1:CDS
GCGGAACCAATCATGACTCG+CGG 0.676119 1:+53624098 MsaT003223.1:CDS
CTCTCATTATGAGACTCACA+GGG 0.677592 1:-53622302 None:intergenic
GTTCCTGGATAGGATCACAA+AGG 0.678518 1:+53624251 MsaT003223.1:intron
CATCATACCAAAGTTGATGG+TGG 0.683172 1:+53623566 MsaT003223.1:CDS
TGTCACGTTCCAAATTGCGG+CGG 0.703136 1:+53624079 MsaT003223.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 53621724 53624374 53621724 ID=MsaG003223
Chr1 mRNA 53621724 53624374 53621724 ID=MsaT003223.1;Parent=MsaG003223
Chr1 exon 53621724 53622725 53621724 ID=MsaT003223.1.exon1;Parent=MsaT003223.1
Chr1 CDS 53621724 53622725 53621724 ID=cds.MsaT003223.1;Parent=MsaT003223.1
Chr1 exon 53622827 53623270 53622827 ID=MsaT003223.1.exon2;Parent=MsaT003223.1
Chr1 CDS 53622827 53623270 53622827 ID=cds.MsaT003223.1;Parent=MsaT003223.1
Chr1 exon 53623356 53624176 53623356 ID=MsaT003223.1.exon3;Parent=MsaT003223.1
Chr1 CDS 53623356 53624176 53623356 ID=cds.MsaT003223.1;Parent=MsaT003223.1
Chr1 exon 53624263 53624374 53624263 ID=MsaT003223.1.exon4;Parent=MsaT003223.1
Chr1 CDS 53624263 53624374 53624263 ID=cds.MsaT003223.1;Parent=MsaT003223.1
Gene Sequence

>MsaT003223.1

ATGACATCTGAAGTAAGAAATAGTGCTAGTGATTGGCACTTAGAGCTTCTTAAAGATTACACTTCTACATATGAAGATGATGAATATAGAAATTCATTTAGCTTTCAAGAGCCACATCAAGAACCTCAGCCTGGAACTAGTACTGTTCATGAATGGGTTCCTTTTGAAGGACAAATTTTTAACACTGATGATGATGCCTATGAATTTTACAATGTATTTGCACGAAAAAATGGATTTTCGATTAGGCGTGATCGCATTTGCAAATCTCATAAAAATGAGTCTGAAGATAATCCATCAGGAGTTTATAAAAGAGAATTTGTTTGTCATCGAGCTGGTATTGTTAAGCAACGCAAAGTTGATGAAGTGAAAAATCAAAGGAAACGGAAATCTTCGCGATGTAGTTGTAGCGCAAAGTTGTTGGTTTCTAAAGGCTTTGAAGGAAAATGGGTGGTTAAATATTTTAATAATAACCACAACCATGAGTTATTAGATGATAAGGAGGTTCAATTTCTTCCCGCTTATCGTAACATCCCAATTGACGATCAAAATCGTATATTATTGTTATCAAAAGCAGGTTGCCCTGTGAGTCTCATAATGAGAGTGCTTGAGTTAGAGAAATCAATAGATACAGGTAACTTACCATTTCTGGAGAAGGATATTAGAAATTTTATTCAATCACACAGTGGTATTGATAAAGAAAATGATGCTTCAGATGTTCTCAAATTATGTAAAAGTCTGAAAGATATGGATAGTGCATTTGAATATGATTTCACAATAGATGAAAGTAACAAACTAGAGCATATTATTTGGGCATTTGGTGATTCAATTCGAGCATATGAATCTTTTGGTGATGTGGTTGTTTTCGATACTACATATCGAATAAACCGTTATGATATGCCACTTGGAATGTGGATTGGGGTTGATAATCATGGAAATTCAATTTTCTTTGGCTGTGTTCTATTGCGAAATGAGAAGAAATCTTCATTCAGATGGGCTTTAAAGAGTTTTCTAACTTTTGTTGAAGGAAAATATCCACAAACAATCCTTACGGATCAAGATCTTGCACTTACAGAAGCCATTGCAAAGGAATTGCCAAATACAAAACATGCATTTTGCATATGGCATATTGTTGCAAAGTTGTCTAGTTGGTTTTCTTTTCCGTTGGGTTCGAGATATATTGACTTTAAACAGGAGTTTCATAGGGTGTACCATTTAGAATCTATAGATGATTTTGAACGAGAATGGGAATTAATGGTTGCACAATTTGGTCTAAGCAAGGACGATCATATTGACTTGCTCTACTCTCATCGTCAATTTTGGGGCCTAGCTTATTTGAAGGACTATTTTTTTGCTGGGATGACGACAACTGGACGTTCTGAATCAATCAACTCGTATGTAAAAAGATTCTTAGATGTGAAAACAAGTTTGACTGATTTTGTAAATCAGGTTGGTGTTGCTGTCAAAATTAGACATCAAGCAGGAGAAGAGGCAAGAATGCGTCATAAGTACCATAATTCTCTAATTAGGACAACTTTTCCTATTGAGGAACATGCTGCAAAAATACTCACTCCATATGCGTTTGAGTTAATCCAACATGAGATTGAATTGTCTACAAAATATGCTGCCATTCATACAGGTAACAATTATATCGTGCGACATCATACCAAAGTTGATGGTGGTCGTCTAGTAAGCTGGATAGAAGAAGAAGAATCAATTTCTTGTTCTTGTAAAGAATTTGAGTTTTCTGGAATATTGTGCAGACATGCCATTCGAGTGCTACTTGTAAAAAATTATTTTCACTTCCCATCAAAATATCTTCCTTTTCGATGGAGAAGAGAAAGTTCATTGATCCCAAAATGCAGTCATATAGTCAAATGCAATAATGGTTCTCATGTTGAGTTTCAGTCTCTAATTCGATGTTTGGAAGTTGAATCATTAAAGACAAAAGAGAGGGAACAAGTTGCTACTGCAGAGTTAGAGAAATTCCTCCGACAGATAAAAGGCATGCCTGCAAGTCAAGATAATTTGATTTATTTGGAACCCAATGTACCCGATGTTCCAAATAATGATGGTTATGATGTTGAAAATCCTGTTGTTTCAAAATCCAAAGGTCGGCCAAAAGGTTCAAGGCCAAAAGGTGGAGTTGAAGCTGCCAAGAAGCTTCGACGTTGTCACGTTCCAAATTGCGGCGGAACCAATCATGACTCGCGGAATTGTCCAAACAAAAAGAAAAATATTGAAGTATTGCCTTCTCAATCTCCTAATAAGATCACAAAGGGTTGGAGACAGTCATCTTCAAATGTGTGTGAGTGGCGTAAGAAAATGCCTACAATTGTTCTTAAGTTGAAGATAATGAATATTGGATGGTTCTTGCTGTAG
Protein sequence

>MsaT003223.1

MTSEVRNSASDWHLELLKDYTSTYEDDEYRNSFSFQEPHQEPQPGTSTVHEWVPFEGQIFNTDDDAYEFYNVFARKNGFSIRRDRICKSHKNESEDNPSGVYKREFVCHRAGIVKQRKVDEVKNQRKRKSSRCSCSAKLLVSKGFEGKWVVKYFNNNHNHELLDDKEVQFLPAYRNIPIDDQNRILLLSKAGCPVSLIMRVLELEKSIDTGNLPFLEKDIRNFIQSHSGIDKENDASDVLKLCKSLKDMDSAFEYDFTIDESNKLEHIIWAFGDSIRAYESFGDVVVFDTTYRINRYDMPLGMWIGVDNHGNSIFFGCVLLRNEKKSSFRWALKSFLTFVEGKYPQTILTDQDLALTEAIAKELPNTKHAFCIWHIVAKLSSWFSFPLGSRYIDFKQEFHRVYHLESIDDFEREWELMVAQFGLSKDDHIDLLYSHRQFWGLAYLKDYFFAGMTTTGRSESINSYVKRFLDVKTSLTDFVNQVGVAVKIRHQAGEEARMRHKYHNSLIRTTFPIEEHAAKILTPYAFELIQHEIELSTKYAAIHTGNNYIVRHHTKVDGGRLVSWIEEEESISCSCKEFEFSGILCRHAIRVLLVKNYFHFPSKYLPFRWRRESSLIPKCSHIVKCNNGSHVEFQSLIRCLEVESLKTKEREQVATAELEKFLRQIKGMPASQDNLIYLEPNVPDVPNNDGYDVENPVVSKSKGRPKGSRPKGGVEAAKKLRRCHVPNCGGTNHDSRNCPNKKKNIEVLPSQSPNKITKGWRQSSSNVCEWRKKMPTIVLKLKIMNIGWFLL*