AlfalfaGEDB | MsaG003287

M. sativa ssp. caerulea / MsaG003287

KEGG: K00924 K08959 K08960
GO: GO:0000226 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005911 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0008092 GO:0008150 GO:0008152 GO:0008360 GO:0009506 GO:0009987 GO:0012505 GO:0015630 GO:0015631 GO:0016043 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0023052 GO:0030054 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0036211 GO:0040008 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0046777 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0051510 GO:0051716 GO:0055028 GO:0055044 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0097435 GO:0098657 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003287	XP_039683963.1	98.884	448	5	0	1	448	1	448	0.0	925

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003287	sp\|Q8LPJ1\|CKL6_ARATH	72.594	478	98	11	1	448	5	479	0.0	679

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003287	tr\|A0A072VLK3\|A0A072VLK3_MEDTR	97.577	454	5	1	1	448	1	454	0.0	919

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000034	MsaG003287	0.816331	1.014297e-51	2.028080e-49
MsaG001156	MsaG003287	0.821381	7.314482e-53	1.668576e-50
MsaG001178	MsaG003287	0.816270	1.046534e-51	2.089276e-49
MsaG001179	MsaG003287	0.832687	1.484134e-55	4.642222e-53
MsaG002016	MsaG003287	0.852872	6.664714e-61	3.991675e-58
MsaG002218	MsaG003287	0.818584	3.168919e-52	6.714966e-50
MsaG003083	MsaG003287	0.821436	7.103293e-53	1.622751e-50
MsaG003276	MsaG003287	0.870408	3.039730e-66	3.583278e-63
MsaG003287	MsaG003288	0.876396	3.005144e-68	4.599061e-65
MsaG003287	MsaG003394	0.801303	1.607423e-48	2.238228e-46
MsaG003287	MsaG004282	0.829271	1.013676e-54	2.872858e-52
MsaG003287	MsaG004726	0.801890	1.220023e-48	1.721655e-46
MsaG003287	MsaG005001	0.804330	3.838692e-49	5.730039e-47
MsaG003287	MsaG006533	0.805733	1.958637e-49	3.020576e-47
MsaG003287	MsaG007067	0.804610	3.357313e-49	5.043909e-47
MsaG003287	MsaG007423	0.813374	4.554869e-51	8.452562e-49
MsaG003287	MsaG007428	0.812750	6.232561e-51	1.138707e-48
MsaG003287	MsaG008538	0.815879	1.277868e-51	2.525859e-49
MsaG003287	MsaG010095	0.827649	2.486129e-54	6.732102e-52
MsaG003287	MsaG010107	0.807096	1.013992e-49	1.614828e-47
MsaG003287	MsaG011505	0.838267	5.850436e-57	2.163485e-54
MsaG003287	MsaG011598	0.825321	8.867815e-54	2.250541e-51
MsaG003287	MsaG012326	0.802944	7.416822e-49	1.072149e-46
MsaG003287	MsaG012933	0.805786	1.910079e-49	2.949260e-47
MsaG003287	MsaG012941	0.804321	3.853751e-49	5.751396e-47
MsaG003287	MsaG012943	0.805540	2.149429e-49	3.299751e-47
MsaG003287	MsaG013909	0.811823	9.910496e-51	1.769627e-48
MsaG003287	MsaG014422	0.808681	4.683441e-50	7.747916e-48
MsaG003287	MsaG015173	0.809121	3.773724e-50	6.309385e-48
MsaG003287	MsaG016724	0.812772	6.166320e-51	1.127199e-48
MsaG003287	MsaG016685	0.813077	5.289145e-51	9.742713e-49
MsaG003287	MsaG016920	0.804420	3.676954e-49	5.499964e-47
MsaG003287	MsaG018183	0.811550	1.135557e-50	2.014081e-48
MsaG003287	MsaG018452	0.834913	4.146158e-56	1.385188e-53
MsaG003287	MsaG018553	0.836908	1.300763e-56	4.614051e-54
MsaG003287	MsaG019182	0.814943	2.059690e-51	3.975805e-49
MsaG003287	MsaG020314	0.843461	2.578020e-58	1.123507e-55
MsaG003287	MsaG020450	0.800755	2.078546e-48	2.858657e-46
MsaG003287	MsaG020983	0.816422	9.676502e-52	1.939290e-49
MsaG003287	MsaG020984	0.825857	6.630344e-54	1.707865e-51
MsaG003287	MsaG021088	0.813986	3.346146e-51	6.305513e-49
MsaG003287	MsaG021405	0.823363	2.546890e-53	6.127836e-51
MsaG003287	MsaG021575	0.811879	9.640097e-51	1.723682e-48
MsaG003287	MsaG021896	0.818276	3.719000e-52	7.817823e-50
MsaG003287	MsaG022059	0.805908	1.800483e-49	2.788061e-47
MsaG003287	MsaG022683	0.801638	1.373753e-48	1.927455e-46
MsaG003287	MsaG022923	0.800508	2.332699e-48	3.190320e-46
MsaG003287	MsaG023744	0.819207	2.291099e-52	4.934932e-50
MsaG003287	MsaG023683	0.829739	7.809301e-55	2.243247e-52
MsaG003287	MsaG023856	0.813987	3.344432e-51	6.302469e-49
MsaG003287	MsaG025691	0.802658	8.495053e-49	1.219997e-46
MsaG003287	MsaG028456	0.813524	4.223534e-51	7.867374e-49
MsaG003287	MsaG028457	0.806899	1.115766e-49	1.768658e-47
MsaG003287	MsaG029388	0.801803	1.271233e-48	1.790341e-46
MsaG003287	MsaG029340	0.809753	2.765647e-50	4.694903e-48
MsaG003287	MsaG030840	0.833199	1.108543e-55	3.520117e-53
MsaG003287	MsaG031277	0.800214	2.676161e-48	3.635699e-46
MsaG003287	MsaG031436	0.838792	4.288934e-57	1.612264e-54
MsaG003287	MsaG033354	0.812759	6.206225e-51	1.134130e-48
MsaG003287	MsaG033369	0.808100	6.220926e-50	1.014814e-47
MsaG003287	MsaG033371	0.817238	6.361341e-52	1.302014e-49
MsaG003287	MsaG033437	0.811454	1.190993e-50	2.107468e-48
MsaG003287	MsaG034287	0.815550	1.511976e-51	2.963743e-49
MsaG003287	MsaG035210	0.801233	1.661254e-48	2.309461e-46
MsaG003287	MsaG035270	0.809900	2.572104e-50	4.381909e-48
MsaG003287	MsaG036217	0.816491	9.343507e-52	1.875854e-49
MsaG003287	MsaG038415	0.814768	2.250724e-51	4.325548e-49
MsaG003287	MsaG038986	0.803358	6.095886e-49	8.896713e-47
MsaG003287	MsaG039739	0.809009	3.987784e-50	6.649192e-48
MsaG003287	MsaG040077	0.821430	7.125286e-53	1.627514e-50
MsaG003287	MsaG040740	0.817425	5.776778e-52	1.188036e-49
MsaG003287	MsaG040775	0.838647	4.674974e-57	1.749380e-54
MsaG003287	MsaG040994	0.828604	1.467616e-54	4.082117e-52
MsaG003287	MsaG042086	0.824679	1.255189e-53	3.129784e-51
MsaG003287	MsaG043665	0.854002	3.168223e-61	1.975321e-58
MsaG003287	MsaG044131	0.823612	2.228914e-53	5.399181e-51
MsaG003287	MsaG044284	0.813370	4.565010e-51	8.470388e-49
MsaG003287	MsaG044323	0.808952	4.101498e-50	6.829408e-48
MsaG003287	MsaG044352	0.838781	4.317421e-57	1.622392e-54
MsaG003287	MsaG044354	0.845437	7.625925e-59	3.545035e-56
MsaG003287	MsaG044606	0.807048	1.037843e-49	1.650944e-47
MsaG003287	MsaG045125	0.828235	1.799936e-54	4.954678e-52
MsaG003287	MsaG046307	0.817120	6.762546e-52	1.379881e-49
MsaG003287	MsaG046384	0.801758	1.298397e-48	1.826702e-46
MsaG003287	MsaG003538	0.800792	2.042268e-48	2.811200e-46
MsaG003287	MsaG008553	0.820353	1.257367e-52	2.791488e-50
MsaG003287	MsaG008937	0.807995	6.548607e-50	1.065585e-47
MsaG003287	MsaG010026	0.805515	2.175210e-49	3.337380e-47
MsaG003287	MsaG019920	0.801050	1.810452e-48	2.506458e-46
MsaG003287	MsaG028544	0.803057	7.030971e-49	1.019032e-46
MsaG003287	MsaG027786	0.800209	2.681665e-48	3.642803e-46
MsaG003287	MsaG032859	0.800219	2.669613e-48	3.627273e-46
MsaG003287	MsaG033582	0.827204	3.176132e-54	8.493495e-52
MsaG003287	MsaG036352	0.804830	3.022498e-49	4.564268e-47
MsaG003287	MsaG038716	0.808285	5.684942e-50	9.315102e-48
MsaG003287	MsaG035298	0.807654	7.730657e-50	1.247624e-47
MsaG003287	MsaG035677	0.801083	1.782131e-48	2.469150e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003287	MtrunA17_Chr1g0183631	97.577	454	5	1	1	448	1	454	0.0	919
MsaG003287	MtrunA17_Chr7g0261501	78.065	465	83	7	1	448	1	463	0.0	726
MsaG003287	MtrunA17_Chr1g0187571	71.304	460	115	6	1	446	2	458	0.0	641
MsaG003287	MtrunA17_Chr1g0166821	71.145	454	120	5	1	446	2	452	0.0	632
MsaG003287	MtrunA17_Chr2g0329981	65.358	433	114	8	1	425	1	405	0.0	551
MsaG003287	MtrunA17_Chr4g0071031	72.222	360	87	4	1	347	1	360	0.0	545
MsaG003287	MtrunA17_Chr8g0342821	59.958	472	154	10	1	444	1	465	0.0	540
MsaG003287	MtrunA17_Chr2g0299661	55.274	474	176	6	1	447	1	465	0.0	516
MsaG003287	MtrunA17_Chr6g0486611	76.797	306	71	0	1	306	1	306	2.51e-179	508
MsaG003287	MtrunA17_Chr1g0167261	66.935	372	90	4	1	370	2	342	6.97e-178	501
MsaG003287	MtrunA17_Chr8g0390211	77.181	298	68	0	1	298	1	298	3.13e-177	502
MsaG003287	MtrunA17_Chr3g0140071	55.582	421	171	4	1	417	1	409	3.06e-164	469
MsaG003287	MtrunA17_Chr1g0146151	70.508	295	87	0	1	295	1	295	1.34e-162	465
MsaG003287	MtrunA17_Chr7g0214531	37.716	289	161	6	5	277	31	316	9.44e-58	200
MsaG003287	MtrunA17_Chr3g0140431	37.276	279	162	6	5	273	32	307	6.90e-55	192
MsaG003287	MtrunA17_Chr1g0146301	36.918	279	163	6	5	273	118	393	3.86e-54	191
MsaG003287	MtrunA17_Chr1g0213321	36.268	284	167	6	5	277	113	393	1.13e-53	190
MsaG003287	MtrunA17_Chr7g0253211	36.525	282	163	5	9	277	324	602	6.49e-53	190
MsaG003287	MtrunA17_Chr8g0340491	36.170	282	158	7	9	273	128	404	9.78e-50	179
MsaG003287	MtrunA17_Chr5g0405421	26.818	220	144	6	8	219	98	308	3.11e-14	74.7
MsaG003287	MtrunA17_Chr7g0267751	29.167	216	131	9	9	215	135	337	9.08e-14	73.6
MsaG003287	MtrunA17_Chr4g0072831	32.680	153	71	6	7	153	8	134	2.91e-13	70.5
MsaG003287	MtrunA17_Chr3g0107051	29.817	218	133	9	5	214	17	222	8.51e-13	70.1
MsaG003287	MtrunA17_Chr7g0245031	34.375	128	70	4	109	236	252	365	1.92e-12	68.9
MsaG003287	MtrunA17_Chr8g0346071	30.415	217	126	9	7	213	15	216	1.01e-11	66.6
MsaG003287	MtrunA17_Chr7g0267771	26.522	230	142	8	9	228	88	300	1.06e-11	66.6
MsaG003287	MtrunA17_Chr5g0444171	27.488	211	137	6	9	213	92	292	2.81e-11	65.5
MsaG003287	MtrunA17_Chr8g0374701	27.948	229	139	10	6	219	122	339	3.10e-11	65.5
MsaG003287	MtrunA17_Chr1g0201571	28.302	212	127	9	9	209	126	323	3.46e-11	65.5
MsaG003287	MtrunA17_Chr1g0157131	27.652	264	158	10	4	251	69	315	5.09e-11	64.7
MsaG003287	MtrunA17_Chr4g0075541	25.887	282	179	10	11	281	89	351	5.17e-11	64.3
MsaG003287	MtrunA17_Chr8g0387671	29.437	231	134	11	9	228	66	278	6.83e-11	64.3
MsaG003287	MtrunA17_Chr3g0107011	26.070	257	137	10	3	236	8	234	8.99e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003287	AT4G28540.1	72.594	478	98	11	1	448	5	479	0.0	679
MsaG003287	AT4G14340.1	70.861	453	116	7	1	446	7	450	0.0	642
MsaG003287	AT3G23340.1	71.938	449	110	7	1	446	1	436	0.0	642
MsaG003287	AT1G03930.1	68.354	474	121	8	1	448	1	471	0.0	626
MsaG003287	AT5G43320.1	67.579	475	124	7	1	446	1	474	0.0	625
MsaG003287	AT3G23340.2	69.265	449	108	8	1	446	1	422	0.0	608
MsaG003287	AT1G04440.1	66.164	464	137	8	1	446	1	462	0.0	599
MsaG003287	AT5G44100.1	82.000	350	58	2	1	346	1	349	0.0	597
MsaG003287	AT5G44100.2	82.000	350	58	2	1	346	1	349	0.0	597
MsaG003287	AT4G14340.2	83.537	328	53	1	1	328	7	333	0.0	576
MsaG003287	AT5G43320.2	81.024	332	60	1	1	329	1	332	0.0	565
MsaG003287	AT5G43320.3	81.024	332	60	1	1	329	1	332	0.0	565
MsaG003287	AT1G72710.1	75.152	330	79	2	1	328	1	329	0.0	536
MsaG003287	AT4G26100.1	80.872	298	57	0	1	298	1	298	0.0	535
MsaG003287	AT5G57015.1	80.537	298	58	0	1	298	1	298	0.0	529
MsaG003287	AT2G19470.1	73.556	329	84	3	1	326	1	329	0.0	524
MsaG003287	AT4G28880.3	71.160	319	92	0	1	319	1	319	7.65e-176	499
MsaG003287	AT4G28880.2	71.160	319	92	0	1	319	1	319	7.65e-176	499
MsaG003287	AT4G28880.1	71.160	319	92	0	1	319	1	319	7.65e-176	499
MsaG003287	AT4G28880.4	71.160	319	92	0	1	319	1	319	7.65e-176	499
MsaG003287	AT4G28860.2	70.533	319	94	0	1	319	1	319	1.08e-174	494
MsaG003287	AT4G28860.1	74.832	298	75	0	1	298	1	298	2.02e-174	495
MsaG003287	AT4G08800.1	66.216	296	68	2	1	296	1	264	3.73e-145	416
MsaG003287	AT2G25760.2	39.286	280	155	4	9	273	107	386	7.33e-59	204
MsaG003287	AT2G25760.1	38.929	280	153	5	9	273	107	383	3.87e-56	197
MsaG003287	AT3G13670.1	35.986	289	166	6	5	277	131	416	3.68e-53	189
MsaG003287	AT5G18190.2	35.461	282	166	5	9	277	130	408	1.84e-52	187
MsaG003287	AT5G18190.1	35.461	282	166	5	9	277	130	408	1.84e-52	187
MsaG003287	AT3G03940.1	35.461	282	166	5	9	277	140	418	4.67e-52	186
MsaG003287	AT3G50530.1	28.319	226	141	7	5	219	144	359	1.10e-13	73.6
MsaG003287	AT5G21326.1	29.870	231	113	10	7	214	11	215	3.38e-13	71.6
MsaG003287	AT3G20410.2	28.384	229	143	8	9	230	91	305	5.47e-12	68.2
MsaG003287	AT3G20410.1	28.384	229	143	8	9	230	91	305	5.47e-12	68.2
MsaG003287	AT2G17890.1	25.909	220	146	7	8	219	107	317	5.77e-12	68.2
MsaG003287	AT1G30270.1	27.386	241	147	10	7	236	29	252	6.34e-12	67.8
MsaG003287	AT1G30270.2	27.386	241	147	10	7	236	29	252	6.50e-12	67.8
MsaG003287	AT2G38910.1	28.063	253	144	10	1	230	119	356	1.07e-11	67.4
MsaG003287	AT3G50530.2	26.104	249	148	9	5	219	144	390	1.42e-11	67.0
MsaG003287	AT4G36070.2	25.000	220	148	6	8	219	70	280	1.47e-11	67.0
MsaG003287	AT4G36070.1	25.000	220	148	6	8	219	70	280	1.80e-11	66.6
MsaG003287	AT3G61960.2	24.843	318	204	10	7	318	8	296	1.89e-11	66.6
MsaG003287	AT3G61960.1	24.843	318	204	10	7	318	8	296	2.02e-11	66.6
MsaG003287	AT4G31170.7	28.319	226	128	8	8	218	130	336	2.86e-11	65.5
MsaG003287	AT4G31170.6	28.319	226	128	8	8	218	130	336	2.86e-11	65.5
MsaG003287	AT4G31170.5	28.319	226	128	8	8	218	130	336	2.86e-11	65.5
MsaG003287	AT4G31170.1	28.319	226	128	8	8	218	130	336	2.86e-11	65.5
MsaG003287	AT4G31170.2	28.319	226	128	8	8	218	130	336	2.86e-11	65.5
MsaG003287	AT4G31170.3	28.319	226	128	8	8	218	130	336	2.86e-11	65.5
MsaG003287	AT4G31170.8	28.319	226	128	8	8	218	130	336	2.86e-11	65.5
MsaG003287	AT1G29230.1	27.397	219	141	9	2	213	67	274	2.91e-11	65.9
MsaG003287	AT1G01140.1	28.689	244	140	13	7	236	17	240	3.12e-11	65.5
MsaG003287	AT1G01140.3	28.689	244	140	13	7	236	17	240	3.32e-11	65.5
MsaG003287	AT1G50700.3	25.597	293	192	10	9	293	73	347	4.36e-11	65.1
MsaG003287	AT1G76040.3	27.586	232	147	8	9	233	112	329	4.65e-11	65.1
MsaG003287	AT1G76040.2	28.037	214	136	7	9	215	112	314	4.68e-11	65.5
MsaG003287	AT4G23650.1	29.167	216	131	7	9	215	78	280	6.36e-11	64.7
MsaG003287	AT1G50700.1	25.597	293	192	10	9	293	73	347	6.56e-11	64.7
MsaG003287	AT4G04720.2	23.052	308	201	9	2	293	66	353	8.09e-11	64.3
MsaG003287	AT4G04720.1	23.052	308	201	9	2	293	66	353	8.09e-11	64.3
MsaG003287	AT1G50700.2	25.357	280	184	9	9	281	73	334	8.96e-11	64.3

Find 121 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTAAACTTGGAAGGAAAAT+TGG	0.141980	1:-54356445	MsaT003287.1:CDS
TAGAATTTGCGCGGTTCCTC+TGG	0.208716	1:+54350628	None:intergenic
GAATGGTCACCTGAGGAATT+TGG	0.252408	1:+54350081	None:intergenic
CCGACTTCAAAGTGAATTTC+CGG	0.284081	1:+54355584	None:intergenic
ACATACCTTCTCGAATAAAA+AGG	0.309439	1:+54351188	None:intergenic
CTCTTTAAATATGAATAATC+TGG	0.309704	1:+54351223	None:intergenic
CAAGTCATAATTACCTCTAT+TGG	0.319914	1:+54352540	None:intergenic
AAAATTGGGAGTGGTTCTTT+TGG	0.322856	1:-54356430	MsaT003287.1:CDS
TTCAGTTGGGAGAGAGATTC+GGG	0.329420	1:-54350685	MsaT003287.1:intron
CAACACAATTCAACTCGATA+TGG	0.357353	1:-54350138	MsaT003287.1:CDS
CATAGCGTGCCGTGCCTGTA+AGG	0.359631	1:+54352934	None:intergenic
AATTCCTCAGGTGACCATTC+TGG	0.359748	1:-54350078	MsaT003287.1:CDS
ATTTATTCAATTATTGTAAC+CGG	0.360177	1:-54355603	MsaT003287.1:CDS
GTGCTAATGTATTTCTTAAG+AGG	0.362458	1:-54352723	MsaT003287.1:CDS
TTAAACTTGGAAGGAAAATT+GGG	0.363410	1:-54356444	MsaT003287.1:CDS
TCCAGCCCTCAGTCCTTGCC+AGG	0.368539	1:+54352611	None:intergenic
AGGGAAAATAAGAACCTTAC+AGG	0.372840	1:-54352948	MsaT003287.1:CDS
CAGGTGTATGCCATTGATTT+CGG	0.375174	1:-54353308	MsaT003287.1:CDS
TTTCAGTTGGGAGAGAGATT+CGG	0.379707	1:-54350686	MsaT003287.1:intron
GTCATGGTCATCGACCTTCT+TGG	0.387600	1:-54355640	MsaT003287.1:CDS
CGACCTTCTTGGACCCAGTC+TGG	0.390528	1:-54355629	MsaT003287.1:CDS
ATGATTCTTCAAGGAGGAAC+TGG	0.404829	1:-54355706	MsaT003287.1:CDS
ACCTCAACGAACGGCAATAG+TGG	0.405356	1:+54351257	None:intergenic
CCTCAACTCCAAACCATTTG+AGG	0.409202	1:+54355674	None:intergenic
GCTTTGAGCTCCTCACTATC+AGG	0.414666	1:-54349930	MsaT003287.1:CDS
GAGATTCGGGAGAAATTATC+TGG	0.415618	1:-54350672	MsaT003287.1:CDS
CTCAAATGGTTTGGAGTTGA+GGG	0.423483	1:-54355673	MsaT003287.1:CDS
TCAGCTGTTAATATACAAAC+TGG	0.426788	1:-54356290	MsaT003287.1:intron
GCAAGTGTCAACACCCATCT+TGG	0.433561	1:-54352912	MsaT003287.1:CDS
ATAGGGATCTTCAGACTCAT+AGG	0.434481	1:-54353271	MsaT003287.1:CDS
TTTATCATGGATCATGTGAT+TGG	0.443086	1:-54356475	None:intergenic
CCTCAAATGGTTTGGAGTTG+AGG	0.448526	1:-54355674	MsaT003287.1:CDS
TTGCCGTTCGTTGAGGTTTG+AGG	0.451388	1:-54351251	MsaT003287.1:CDS
TTTCGCAAGGCCGAAATCAA+TGG	0.457496	1:+54353298	None:intergenic
ATATCGAGTTGAATTGTGTT+GGG	0.458129	1:+54350140	None:intergenic
ACAAAGTCGAAGGGATGATC+TGG	0.458546	1:-54352760	MsaT003287.1:CDS
ATTGAATAAATCTTCCAGAC+TGG	0.465309	1:+54355615	None:intergenic
CATAAAGCACAAAAGATTCC+AGG	0.469567	1:-54350891	MsaT003287.1:intron
AAACTGTATATGATTCTTCA+AGG	0.471640	1:-54355715	MsaT003287.1:CDS
GTGGAAAGTTTAAACTTGGA+AGG	0.472881	1:-54356453	MsaT003287.1:CDS
CTCCAAACCATTTGAGGTGG+GGG	0.473526	1:+54355680	None:intergenic
GGCAGGATGGCCACAAGTGG+GGG	0.486821	1:-54350057	MsaT003287.1:CDS
ATAGTGAAGCTGTGGATGCC+TGG	0.493685	1:+54355744	None:intergenic
TCTTCCCTGGCAAGGACTGA+GGG	0.499281	1:-54352616	MsaT003287.1:CDS
TTGTGGCCATCCTGCCAGAA+TGG	0.501550	1:+54350064	None:intergenic
CCCTGGCAAGGACTGAGGGC+TGG	0.502421	1:-54352612	MsaT003287.1:CDS
GTCAACACCCATCTTGGAAT+CGG	0.505171	1:-54352906	MsaT003287.1:intron
ATCATCATAAGTCCTATGTC+TGG	0.505727	1:+54350584	None:intergenic
CATATCGAGTTGAATTGTGT+TGG	0.505789	1:+54350139	None:intergenic
ATTGGTGGAAAGTTTAAACT+TGG	0.508093	1:-54356457	MsaT003287.1:CDS
AGAGGAGGTGGCTGTTAAGC+TGG	0.511655	1:-54356267	MsaT003287.1:intron
AGAATTTGCGCGGTTCCTCT+GGG	0.513807	1:+54350629	None:intergenic
CTGGCAGGATGGCCACAAGT+GGG	0.513957	1:-54350059	MsaT003287.1:CDS
AGGGATGATCTGGAATCTCT+TGG	0.515030	1:-54352750	MsaT003287.1:CDS
GAAGGTATCAACTCCAATAG+AGG	0.515426	1:-54352553	MsaT003287.1:intron
GTCTTCCCTGGCAAGGACTG+AGG	0.516641	1:-54352617	MsaT003287.1:CDS
AATTGTCTGGCTTTATGTCT+CGG	0.521040	1:+54355398	None:intergenic
ATCACTTACCGATTCCAAGA+TGG	0.522339	1:+54352898	None:intergenic
TCCACTATTGCCGTTCGTTG+AGG	0.522705	1:-54351258	MsaT003287.1:CDS
CAGAGTAGTGGCAGCTCTAG+AGG	0.523385	1:-54351033	MsaT003287.1:CDS
TCACTTACCGATTCCAAGAT+GGG	0.528445	1:+54352899	None:intergenic
AGGCCCATCAGAAAATTGTC+TGG	0.529417	1:+54355385	None:intergenic
AACTCCAAACCATTTGAGGT+GGG	0.533255	1:+54355678	None:intergenic
TGGCAGGATGGCCACAAGTG+GGG	0.534769	1:-54350058	MsaT003287.1:CDS
CTCTATGTGCGCCAGATGGA+CGG	0.535759	1:+54350034	None:intergenic
CTTGATTTGCTTTACGTCCG+AGG	0.536022	1:+54355365	None:intergenic
TTGTAACGACAGCATGGCAC+TGG	0.538838	1:-54350945	MsaT003287.1:intron
ATCATGGATCATGTGATTGG+TGG	0.540398	1:-54356472	MsaT003287.1:CDS
TATACAAACTGGAGAGGAGG+TGG	0.543406	1:-54356279	MsaT003287.1:CDS
TTGAATAAATCTTCCAGACT+GGG	0.547330	1:+54355616	None:intergenic
TAATGTATTTCTTAAGAGGA+AGG	0.552033	1:-54352719	MsaT003287.1:intron
TAGTGGCAGCTCTAGAGGAC+GGG	0.553447	1:-54351028	MsaT003287.1:intron
TCTGGCAGGATGGCCACAAG+TGG	0.554722	1:-54350060	MsaT003287.1:CDS
GTAGTGGCAGCTCTAGAGGA+CGG	0.557752	1:-54351029	MsaT003287.1:CDS
GTTGAATACATGCATTCAAG+AGG	0.558652	1:-54355430	MsaT003287.1:CDS
TCATCAAAGCCAAATTCCTC+AGG	0.559975	1:-54350090	MsaT003287.1:CDS
CAACTCCAAACCATTTGAGG+TGG	0.572196	1:+54355677	None:intergenic
TCCAGGAATCTGTGAAGACC+AGG	0.574379	1:-54355762	MsaT003287.1:intron
CCTCAGGTGACCATTCTGGC+AGG	0.574601	1:-54350074	MsaT003287.1:CDS
AGGTGACCATTCTGGCAGGA+TGG	0.577708	1:-54350070	MsaT003287.1:CDS
TGGTGTGTTCACCGTGATGA+GGG	0.579905	1:+54350604	None:intergenic
CTCATAGGCACATACCGTAC+AGG	0.580718	1:-54353256	MsaT003287.1:intron
AGACAGACTTACTAATTGAT+CGG	0.581760	1:+54355549	None:intergenic
CTTGGAAGGAAAATTGGGAG+TGG	0.582542	1:-54356439	MsaT003287.1:CDS
CTTCCAGACTGGGTCCAAGA+AGG	0.585280	1:+54355626	None:intergenic
GATCACAAAACCAACCTGTA+CGG	0.586380	1:+54353242	None:intergenic
GAGATTACTTCCTGATAGTG+AGG	0.590566	1:+54349920	None:intergenic
CCAGCCCTCAGTCCTTGCCA+GGG	0.593406	1:+54352612	None:intergenic
ACTCCAAACCATTTGAGGTG+GGG	0.595250	1:+54355679	None:intergenic
TGAACTCTATGTGCGCCAGA+TGG	0.596034	1:+54350030	None:intergenic
ATTTGGCTTTGATGATACCA+TGG	0.597247	1:+54350098	None:intergenic
TGGTGGCAGAACAAAGTCGA+AGG	0.597531	1:-54352770	MsaT003287.1:intron
GCTTGTTTCGTATCATACAC+AGG	0.597643	1:+54350006	None:intergenic
ACGGTGAACACACCAGACAT+AGG	0.599179	1:-54350596	MsaT003287.1:CDS
GCTCGCAATGGGTCTATGAG+AGG	0.601655	1:-54349976	MsaT003287.1:CDS
CCGGAAATTCACTTTGAAGT+CGG	0.603149	1:-54355584	MsaT003287.1:CDS
CTGGTGTGTTCACCGTGATG+AGG	0.605762	1:+54350603	None:intergenic
AGGAGCTCAAAGCTCCTCAG+AGG	0.611695	1:+54349940	None:intergenic
ACATAGGACTTATGATGATG+TGG	0.615199	1:-54350580	MsaT003287.1:intron
ACGACAGCATGGCACTGGCA+AGG	0.615258	1:-54350940	MsaT003287.1:intron
TTGAAGTATCCACAGAGTAG+TGG	0.619683	1:-54351045	MsaT003287.1:CDS
TTTGACTCATAGTGAAGCTG+TGG	0.620621	1:+54355736	None:intergenic
CGATAAAATCAGTGAGACGA+AGG	0.621170	1:-54352571	MsaT003287.1:CDS
TGTCCTCAAACCTCAACGAA+CGG	0.628598	1:+54351248	None:intergenic
TGAGGGAGAGTACAATGTCA+TGG	0.631924	1:-54355656	MsaT003287.1:CDS
AAATAAGAACCTTACAGGCA+CGG	0.632067	1:-54352943	MsaT003287.1:CDS
GATGAGGGCTAGAATTTGCG+CGG	0.634849	1:+54350619	None:intergenic
TAATATACAAACTGGAGAGG+AGG	0.635465	1:-54356282	MsaT003287.1:CDS
AAGCAGTTCAAGAAGAGCCA+TGG	0.646616	1:-54350115	MsaT003287.1:CDS
CTAGAGCTGCCACTACTCTG+TGG	0.650464	1:+54351036	None:intergenic
AAGTATGATACAAACTCAGA+GGG	0.655305	1:+54351280	None:intergenic
TGTTAATATACAAACTGGAG+AGG	0.660638	1:-54356285	MsaT003287.1:CDS
GAAGTATGATACAAACTCAG+AGG	0.660966	1:+54351279	None:intergenic
GGTGGCAGAACAAAGTCGAA+GGG	0.669079	1:-54352769	MsaT003287.1:intron
TTGTTGAAGCATTCACCCAG+AGG	0.669158	1:-54350644	MsaT003287.1:CDS
CCTGCCAGAATGGTCACCTG+AGG	0.679602	1:+54350074	None:intergenic
GCAAATTCTAGCCCTCATCA+CGG	0.684087	1:-54350615	MsaT003287.1:CDS
AGCTCCTCAGAGGATCATCA+CGG	0.696325	1:+54349950	None:intergenic
GCCACCGTGATGATCCTCTG+AGG	0.725239	1:-54349954	MsaT003287.1:CDS
CTGTATATGATTCTTCAAGG+AGG	0.728537	1:-54355712	MsaT003287.1:CDS
TCCTCAGAGGATCATCACGG+TGG	0.751052	1:+54349953	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	54349924	54356491	54349924	ID=MsaG003287
Chr1	mRNA	54349924	54356491	54349924	ID=MsaT003287.1;Parent=MsaG003287
Chr1	exon	54349924	54350169	54349924	ID=MsaT003287.1.exon13;Parent=MsaT003287.1
Chr1	CDS	54349924	54350169	54349924	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54350581	54350702	54350581	ID=MsaT003287.1.exon12;Parent=MsaT003287.1
Chr1	CDS	54350581	54350702	54350581	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54350892	54350955	54350892	ID=MsaT003287.1.exon11;Parent=MsaT003287.1
Chr1	CDS	54350892	54350955	54350892	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54351029	54351102	54351029	ID=MsaT003287.1.exon10;Parent=MsaT003287.1
Chr1	CDS	54351029	54351102	54351029	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54351194	54351320	54351194	ID=MsaT003287.1.exon9;Parent=MsaT003287.1
Chr1	CDS	54351194	54351320	54351194	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54352554	54352638	54352554	ID=MsaT003287.1.exon8;Parent=MsaT003287.1
Chr1	CDS	54352554	54352638	54352554	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54352720	54352783	54352720	ID=MsaT003287.1.exon7;Parent=MsaT003287.1
Chr1	CDS	54352720	54352783	54352720	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54352907	54352968	54352907	ID=MsaT003287.1.exon6;Parent=MsaT003287.1
Chr1	CDS	54352907	54352968	54352907	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54353257	54353327	54353257	ID=MsaT003287.1.exon5;Parent=MsaT003287.1
Chr1	CDS	54353257	54353327	54353257	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54355366	54355461	54355366	ID=MsaT003287.1.exon4;Parent=MsaT003287.1
Chr1	CDS	54355366	54355461	54355366	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54355561	54355779	54355561	ID=MsaT003287.1.exon3;Parent=MsaT003287.1
Chr1	CDS	54355561	54355779	54355561	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54356268	54356308	54356268	ID=MsaT003287.1.exon2;Parent=MsaT003287.1
Chr1	CDS	54356268	54356308	54356268	ID=cds.MsaT003287.1;Parent=MsaT003287.1
Chr1	exon	54356416	54356491	54356416	ID=MsaT003287.1.exon1;Parent=MsaT003287.1
Chr1	CDS	54356416	54356491	54356416	ID=cds.MsaT003287.1;Parent=MsaT003287.1

Gene Sequence

>MsaT003287.1

ATGGATCATGTGATTGGTGGAAAGTTTAAACTTGGAAGGAAAATTGGGAGTGGTTCTTTTGGAGAGCTCTATTTAGCTGTTAATATACAAACTGGAGAGGAGGTGGCTGTTAAGCTGGAATCTGTGAAGACCAGGCATCCACAGCTTCACTATGAGTCAAAACTGTATATGATTCTTCAAGGAGGAACTGGAATCCCCCACCTCAAATGGTTTGGAGTTGAGGGAGAGTACAATGTCATGGTCATCGACCTTCTTGGACCCAGTCTGGAAGATTTATTCAATTATTGTAACCGGAAATTCACTTTGAAGTCGGTGTTGATGCTTGCCGATCAATTAATTAACAGAGTTGAATACATGCATTCAAGAGGCTTTCTTCACCGAGACATAAAGCCAGACAATTTTCTGATGGGCCTCGGACGTAAAGCAAATCAAGTGTATGCCATTGATTTCGGCCTTGCGAAAAAATATAGGGATCTTCAGACTCATAGGCACATACCGTACAGGGAAAATAAGAACCTTACAGGCACGGCACGCTATGCAAGTGTCAACACCCATCTTGGAATCGAACAAAGTCGAAGGGATGATCTGGAATCTCTTGGTTATGTGCTAATGTATTTCTTAAGAGGAAGTCTTCCCTGGCAAGGACTGAGGGCTGGAAACAAAAAGCAAAAATACGATAAAATCAGTGAGACGAAGGTATCAACTCCAATAGAGGTGCTCTGTAAATCATATCCCTCTGAGTTTGTATCATACTTCCACTATTGCCGTTCGTTGAGGTTTGAGGACAAACCAGATTATTCATATTTAAAGAGACTTTTCCGAGACCTTTTTATTCGAGAAGGCTATCAATTTGACTATGTTTTTGACTGGACTGTATTGAAGTATCCACAGAGTAGTGGCAGCTCTAGAGGACGGCATGGCACTGGCAAGGCAGCTATGCATGCAGCTACACCAGTACATAAAGCACAAAAGATTCCAGTTGGGAGAGAGATTCGGGAGAAATTATCTGGTGCTGTTGTTGAAGCATTCACCCAGAGGAACCGCGCAAATTCTAGCCCTCATCACGGTGAACACACCAGACATAGGACTTATGATGATGTGCACTATGCCCAACACAATTCAACTCGATATGGAAGCAGTTCAAGAAGAGCCATGGTATCATCAAAGCCAAATTCCTCAGGTGACCATTCTGGCAGGATGGCCACAAGTGGGGGCCGTCCATCTGGCGCACATAGAGTTCAACCTGTGTATGATACGAAACAAGCAACTTTTGCTCGCAATGGGTCTATGAGAGGCCACCGTGATGATCCTCTGAGGAGCTTTGAGCTCCTCACTATCAGGAAGTAA

Protein sequence

>MsaT003287.1

MDHVIGGKFKLGRKIGSGSFGELYLAVNIQTGEEVAVKLESVKTRHPQLHYESKLYMILQGGTGIPHLKWFGVEGEYNVMVIDLLGPSLEDLFNYCNRKFTLKSVLMLADQLINRVEYMHSRGFLHRDIKPDNFLMGLGRKANQVYAIDFGLAKKYRDLQTHRHIPYRENKNLTGTARYASVNTHLGIEQSRRDDLESLGYVLMYFLRGSLPWQGLRAGNKKQKYDKISETKVSTPIEVLCKSYPSEFVSYFHYCRSLRFEDKPDYSYLKRLFRDLFIREGYQFDYVFDWTVLKYPQSSGSSRGRHGTGKAAMHAATPVHKAQKIPVGREIREKLSGAVVEAFTQRNRANSSPHHGEHTRHRTYDDVHYAQHNSTRYGSSSRRAMVSSKPNSSGDHSGRMATSGGRPSGAHRVQPVYDTKQATFARNGSMRGHRDDPLRSFELLTIRK*