AlfalfaGEDB | MsaG003406

M. sativa ssp. caerulea / MsaG003406

KEGG: K08967 K09419
GO: GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032268 GO:0042802 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051246 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00447.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003406	XP_039684164.1	90.645	310	26	3	1	309	1	308	0.0	556

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003406	sp\|Q9LUH8\|HFA6B_ARATH	49.254	335	125	8	13	303	41	374	7.49e-94	287

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003406	tr\|A0A072VKS2\|A0A072VKS2_MEDTR	90.645	310	26	3	1	309	1	308	0.0	556

TFs/TRs:

Gene ID	Type	Classification
MsaG003406	TF	HSF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000167	MsaG003406	0.820377	1.241768e-52	2.758556e-50
MsaG000566	MsaG003406	0.834837	4.332910e-56	1.444303e-53
MsaG000761	MsaG003406	0.817389	5.886139e-52	1.209392e-49
MsaG001506	MsaG003406	0.859046	1.062007e-62	7.963592e-60
MsaG001516	MsaG003406	0.819772	1.705303e-52	3.727815e-50
MsaG001633	MsaG003406	0.803326	6.188675e-49	9.025401e-47
MsaG001682	MsaG003406	0.852396	9.095032e-61	5.357494e-58
MsaG002810	MsaG003406	0.803010	7.191073e-49	1.041095e-46
MsaG003406	MsaG003512	0.804399	3.713731e-49	5.552322e-47
MsaG003406	MsaG004091	0.820933	9.268383e-53	2.089392e-50
MsaG003406	MsaG004166	0.821444	7.074081e-53	1.616434e-50
MsaG003406	MsaG004956	0.815670	1.421985e-51	2.795901e-49
MsaG003406	MsaG005054	0.826193	5.519763e-54	1.435145e-51
MsaG003406	MsaG005349	0.824627	1.290685e-53	3.213779e-51
MsaG003406	MsaG005545	0.802366	9.747129e-49	1.390511e-46
MsaG003406	MsaG005814	0.822397	4.266904e-53	1.000137e-50
MsaG003406	MsaG006332	0.819919	1.578957e-52	3.465208e-50
MsaG003406	MsaG007074	0.817762	4.854680e-52	1.007066e-49
MsaG003406	MsaG007077	0.809543	3.067492e-50	5.181057e-48
MsaG003406	MsaG008330	0.858248	1.832454e-62	1.333694e-59
MsaG003406	MsaG008939	0.829045	1.149179e-54	3.236172e-52
MsaG003406	MsaG008941	0.828542	1.519310e-54	4.218542e-52
MsaG003406	MsaG008954	0.818232	3.805595e-52	7.990852e-50
MsaG003406	MsaG008959	0.825763	6.974816e-54	1.791899e-51
MsaG003406	MsaG009518	0.804221	4.042534e-49	6.019184e-47
MsaG003406	MsaG010305	0.805857	1.845588e-49	2.854443e-47
MsaG003406	MsaG011625	0.826061	5.933670e-54	1.537107e-51
MsaG003406	MsaG011678	0.827802	2.286207e-54	6.217341e-52
MsaG003406	MsaG012487	0.823382	2.520840e-53	6.068262e-51
MsaG003406	MsaG012470	0.863212	5.807075e-64	5.110155e-61
MsaG003406	MsaG012592	0.821534	6.745860e-53	1.545118e-50
MsaG003406	MsaG012714	0.842660	4.203079e-58	1.785284e-55
MsaG003406	MsaG013055	0.844759	1.160491e-58	5.275890e-56
MsaG003406	MsaG014135	0.819169	2.337222e-52	5.029210e-50
MsaG003406	MsaG014651	0.825878	6.552954e-54	1.688972e-51
MsaG003406	MsaG015883	0.832082	2.092693e-55	6.432129e-53
MsaG003406	MsaG016056	0.812180	8.292116e-51	1.493666e-48
MsaG003406	MsaG016118	0.824981	1.066363e-53	2.681045e-51
MsaG003406	MsaG016154	0.808183	5.973992e-50	9.764749e-48
MsaG003406	MsaG016459	0.861587	1.824085e-63	1.506632e-60
MsaG003406	MsaG017106	0.811120	1.406775e-50	2.468916e-48
MsaG003406	MsaG017285	0.825281	9.062262e-54	2.297267e-51
MsaG003406	MsaG017608	0.804225	4.034214e-49	6.007364e-47
MsaG003406	MsaG017625	0.862316	1.093949e-63	9.294295e-61
MsaG003406	MsaG017629	0.874875	9.927219e-68	1.419978e-64
MsaG003406	MsaG018114	0.804887	2.941002e-49	4.447085e-47
MsaG003406	MsaG018840	0.809967	2.488456e-50	4.246183e-48
MsaG003406	MsaG019435	0.810100	2.331103e-50	3.990569e-48
MsaG003406	MsaG019568	0.825445	8.290013e-54	2.111056e-51
MsaG003406	MsaG019582	0.800795	2.039708e-48	2.807833e-46
MsaG003406	MsaG020730	0.800718	2.114854e-48	2.906195e-46
MsaG003406	MsaG021164	0.816700	8.390544e-52	1.693613e-49
MsaG003406	MsaG021165	0.804676	3.253655e-49	4.895702e-47
MsaG003406	MsaG021264	0.826031	6.029527e-54	1.560688e-51
MsaG003406	MsaG021680	0.860125	5.046813e-63	3.943115e-60
MsaG003406	MsaG021695	0.830892	4.095498e-55	1.215915e-52
MsaG003406	MsaG022767	0.891647	7.268588e-74	2.335176e-70
MsaG003406	MsaG023214	0.883466	9.372189e-71	1.993146e-67
MsaG003406	MsaG023873	0.801531	1.444727e-48	2.022085e-46
MsaG003406	MsaG024236	0.801812	1.265805e-48	1.783054e-46
MsaG003406	MsaG024728	0.826044	5.988787e-54	1.550692e-51
MsaG003406	MsaG025231	0.830504	5.092727e-55	1.495161e-52
MsaG003406	MsaG028024	0.800327	2.537879e-48	3.456801e-46
MsaG003406	MsaG028188	0.829940	6.980023e-55	2.016687e-52
MsaG003406	MsaG028311	0.804945	2.860176e-49	4.330641e-47
MsaG003406	MsaG029029	0.810734	1.703105e-50	2.960939e-48
MsaG003406	MsaG029384	0.811407	1.219244e-50	2.154954e-48
MsaG003406	MsaG029635	0.843623	2.333707e-58	1.022327e-55
MsaG003406	MsaG029698	0.801226	1.666936e-48	2.316979e-46
MsaG003406	MsaG030089	0.809177	3.671608e-50	6.146691e-48
MsaG003406	MsaG030975	0.805717	1.974606e-49	3.043959e-47
MsaG003406	MsaG031647	0.828102	1.936917e-54	5.312011e-52
MsaG003406	MsaG032594	0.809705	2.831678e-50	4.801438e-48
MsaG003406	MsaG035139	0.827340	2.947493e-54	7.912263e-52
MsaG003406	MsaG036379	0.817667	5.098519e-52	1.055044e-49
MsaG003406	MsaG037421	0.839315	3.143679e-57	1.201078e-54
MsaG003406	MsaG038596	0.808684	4.676886e-50	7.737626e-48
MsaG003406	MsaG038520	0.835466	3.010695e-56	1.022653e-53
MsaG003406	MsaG038522	0.819693	1.777815e-52	3.878186e-50
MsaG003406	MsaG039899	0.836390	1.760147e-56	6.147344e-54
MsaG003406	MsaG040511	0.808117	6.169134e-50	1.006784e-47
MsaG003406	MsaG040640	0.858282	1.790917e-62	1.305141e-59
MsaG003406	MsaG041735	0.866440	5.713819e-65	5.718885e-62
MsaG003406	MsaG041778	0.819567	1.898419e-52	4.127619e-50
MsaG003406	MsaG042022	0.838486	5.139770e-57	1.913733e-54
MsaG003406	MsaG042149	0.844917	1.052252e-58	4.809648e-56
MsaG003406	MsaG042926	0.809889	2.586476e-50	4.405178e-48
MsaG003406	MsaG044050	0.815890	1.270854e-51	2.512682e-49
MsaG003406	MsaG044630	0.814521	2.551171e-51	4.872462e-49
MsaG003406	MsaG044922	0.806415	1.409986e-49	2.209819e-47
MsaG003406	MsaG044923	0.850589	2.936401e-60	1.624178e-57
MsaG003406	MsaG045018	0.811820	9.924818e-51	1.772052e-48
MsaG003406	MsaG045733	0.830002	6.743875e-55	1.951893e-52
MsaG003406	MsaG045757	0.831233	3.379974e-55	1.013366e-52
MsaG003406	MsaG046150	0.814079	3.191776e-51	6.028720e-49
MsaG003406	MsaG046615	0.811503	1.162220e-50	2.059018e-48
MsaG003406	MsaG046740	0.809182	3.663548e-50	6.133879e-48
MsaG003406	MsaG046830	0.830609	4.801498e-55	1.413901e-52
MsaG003406	MsaG046957	0.812290	7.849811e-51	1.417819e-48
MsaG003406	MsaG002644	0.807608	7.905407e-50	1.274414e-47
MsaG003406	MsaG001905	0.831412	3.055326e-55	9.208623e-53
MsaG003406	MsaG011701	0.813246	4.859816e-51	8.989364e-49
MsaG003406	MsaG014700	0.835521	2.917317e-56	9.925683e-54
MsaG003406	MsaG011898	0.827450	2.774561e-54	7.471028e-52
MsaG003406	MsaG023219	0.825573	7.736483e-54	1.977027e-51
MsaG003406	MsaG019826	0.807265	9.341607e-50	1.493618e-47
MsaG003406	MsaG022158	0.813935	3.432887e-51	6.460904e-49
MsaG003406	MsaG026779	0.851580	1.547109e-60	8.854173e-58
MsaG003406	MsaG028048	0.816480	9.393703e-52	1.885402e-49
MsaG003406	MsaG027785	0.846701	3.468024e-59	1.681264e-56
MsaG003406	MsaG031376	0.809946	2.514493e-50	4.288430e-48
MsaG003406	MsaG033463	0.832417	1.730149e-55	5.369459e-53
MsaG003406	MsaG032821	0.834292	5.929289e-56	1.944501e-53
MsaG003406	MsaG033042	0.827493	2.709998e-54	7.306002e-52
MsaG003406	MsaG032454	0.848158	1.385796e-59	7.055190e-57
MsaG003406	MsaG032707	0.908184	5.401688e-81	4.540290e-77
MsaG003406	MsaG030573	0.852246	1.003112e-60	5.877567e-58
MsaG003406	MsaG033446	0.800576	2.259905e-48	3.095574e-46
MsaG003406	MsaG034914	0.822265	4.576807e-53	1.068930e-50
MsaG003406	MsaG036619	0.870925	2.058913e-66	2.481334e-63
MsaG003406	MsaG036344	0.812668	6.494118e-51	1.184053e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003406	MtrunA17_Chr1g0185131	90.645	310	26	3	1	309	1	308	0.0	556
MsaG003406	MtrunA17_Chr7g0260331	54.839	341	108	9	1	309	1	327	5.65e-107	316
MsaG003406	MtrunA17_Chr1g0204741	59.276	221	76	5	20	228	27	245	1.82e-80	247
MsaG003406	MtrunA17_Chr3g0135831	45.000	300	145	5	20	303	33	328	3.37e-76	238
MsaG003406	MtrunA17_Chr4g0039891	51.887	212	83	3	33	227	12	221	2.16e-68	221
MsaG003406	MtrunA17_Chr5g0410881	51.376	218	83	4	31	228	30	244	1.44e-67	219
MsaG003406	MtrunA17_Chr4g0071051	46.364	220	108	4	20	231	88	305	1.13e-61	202
MsaG003406	MtrunA17_Chr6g0467041	43.902	205	96	4	33	226	22	218	6.94e-50	168
MsaG003406	MtrunA17_Chr8g0378061	44.221	199	98	4	34	224	23	216	3.72e-48	168
MsaG003406	MtrunA17_Chr8g0392001	47.090	189	82	3	33	203	22	210	6.25e-47	162
MsaG003406	MtrunA17_Chr8g0379701	36.965	257	132	6	33	263	12	264	6.42e-46	160
MsaG003406	MtrunA17_Chr2g0330821	41.885	191	100	2	23	206	73	259	1.63e-45	158
MsaG003406	MtrunA17_Chr6g0485571	39.111	225	121	4	33	242	11	234	6.88e-44	153
MsaG003406	MtrunA17_Chr5g0397881	65.957	94	32	0	31	124	22	115	1.80e-42	150
MsaG003406	MtrunA17_Chr5g0402291	41.530	183	63	2	31	169	7	189	1.69e-41	145
MsaG003406	MtrunA17_Chr6g0472421	63.830	94	34	0	31	124	31	124	4.97e-41	145
MsaG003406	MtrunA17_Chr1g0203601	63.830	94	34	0	31	124	16	109	3.85e-40	142
MsaG003406	MtrunA17_Chr3g0134031	64.078	103	36	1	31	133	22	123	6.72e-40	143
MsaG003406	MtrunA17_Chr5g0441531	43.860	171	65	3	31	171	23	192	2.79e-39	139
MsaG003406	MtrunA17_Chr7g0257331	45.679	162	64	3	24	161	9	170	1.97e-38	135
MsaG003406	MtrunA17_Chr2g0284481	39.560	182	90	4	33	197	15	193	2.59e-37	137
MsaG003406	MtrunA17_Chr4g0047421	38.764	178	90	4	31	196	31	201	1.67e-33	122
MsaG003406	MtrunA17_Chr8g0375041	53.636	110	50	1	28	137	4	112	4.33e-33	122
MsaG003406	MtrunA17_Chr1g0179311	40.136	147	76	3	90	227	11	154	1.18e-26	107
MsaG003406	MtrunA17_Chr1g0179321	64.384	73	26	0	20	92	36	108	9.66e-25	96.3
MsaG003406	MtrunA17_Chr2g0330841	35.252	139	80	2	93	224	41	176	5.15e-22	95.5
MsaG003406	MtrunA17_Chr4g0023771	55.172	58	26	0	33	90	15	72	5.90e-15	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003406	AT3G22830.1	49.254	335	125	8	13	303	41	374	7.62e-95	287
MsaG003406	AT3G51910.1	52.281	285	90	7	24	303	20	263	8.64e-87	261
MsaG003406	AT3G63350.1	46.333	300	109	8	18	307	13	270	1.65e-78	241
MsaG003406	AT2G26150.1	49.770	217	98	1	20	225	31	247	6.84e-72	226
MsaG003406	AT2G26150.4	49.770	217	98	1	20	225	31	247	6.84e-72	226
MsaG003406	AT1G32330.2	49.533	214	88	1	10	203	14	227	2.52e-65	213
MsaG003406	AT1G32330.1	49.533	214	88	1	10	203	14	227	2.67e-65	213
MsaG003406	AT4G17750.1	44.961	258	109	4	15	242	31	285	6.91e-65	212
MsaG003406	AT5G43840.1	42.606	284	141	7	20	292	6	278	1.17e-64	205
MsaG003406	AT5G16820.1	48.246	228	92	4	14	224	16	234	8.95e-64	209
MsaG003406	AT5G16820.2	48.246	228	92	4	14	224	16	234	8.95e-64	209
MsaG003406	AT3G02990.1	48.325	209	88	3	33	224	23	228	5.79e-61	201
MsaG003406	AT5G03720.2	47.805	205	87	3	33	223	55	253	1.81e-56	188
MsaG003406	AT5G03720.1	47.805	205	87	3	33	223	55	253	1.81e-56	188
MsaG003406	AT1G67970.1	44.907	216	104	3	33	236	19	231	1.99e-53	179
MsaG003406	AT5G54070.1	40.704	199	112	3	33	226	71	268	1.49e-50	171
MsaG003406	AT4G13980.1	43.000	200	104	2	34	226	24	220	1.36e-49	171
MsaG003406	AT3G24520.1	41.451	193	101	2	33	225	17	197	4.27e-45	156
MsaG003406	AT4G36990.1	41.808	177	65	1	31	169	12	188	3.06e-41	145
MsaG003406	AT1G46264.1	38.222	225	80	4	24	190	25	248	4.67e-40	144
MsaG003406	AT5G62020.1	62.766	94	35	0	31	124	21	114	7.76e-39	139
MsaG003406	AT4G18880.1	43.850	187	93	2	23	197	5	191	2.07e-38	140
MsaG003406	AT4G11660.1	62.626	99	36	1	31	129	57	154	4.66e-38	139
MsaG003406	AT5G45710.3	35.714	210	124	2	33	231	13	222	8.93e-37	135
MsaG003406	AT5G45710.1	35.714	210	124	2	33	231	13	222	8.93e-37	135
MsaG003406	AT2G41690.1	62.766	94	35	0	31	124	38	131	5.49e-36	130
MsaG003406	AT2G26150.3	43.357	143	70	1	93	224	49	191	2.89e-32	121
MsaG003406	AT2G26150.2	43.357	143	70	1	93	224	49	191	2.89e-32	121
MsaG003406	AT4G18870.1	52.809	89	37	2	34	120	14	99	9.73e-24	99.0
MsaG003406	AT4G18870.1	46.591	88	47	0	33	120	146	233	1.58e-21	92.8
MsaG003406	AT1G77570.1	35.227	88	54	2	34	118	27	114	8.79e-12	62.8

Find 65 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATTACGTGATTATGAATT+TGG	0.128353	1:-56244029	MsaT003406.1:CDS
ATGCAGAAATTGATCGTTTA+AGG	0.140595	1:-56244349	MsaT003406.1:CDS
ATTATTGTGTTTGAAGTATC+TGG	0.159629	1:+56244700	None:intergenic
TTCTGTTCCATGAAATTATA+TGG	0.235121	1:+56244777	None:intergenic
AACAAGTCCTTCATTTGGTT+GGG	0.266336	1:+56244862	None:intergenic
AATTACGTGATTATGAATTT+GGG	0.282722	1:-56244028	MsaT003406.1:CDS
TTTGCAAATTCCCATCTATC+TGG	0.293795	1:+56244467	None:intergenic
CCTTCATTTGGTTGGGGTGG+TGG	0.306285	1:+56244869	None:intergenic
ACAACAGACAGAGAAGAAAA+AGG	0.341766	1:-56244153	MsaT003406.1:CDS
GAGATGGAACATAGGTTAGA+AGG	0.365971	1:-56244251	MsaT003406.1:CDS
TGCTTGATGATGAAGGGTTC+TGG	0.373815	1:-56243887	MsaT003406.1:CDS
TCATTTGGTTGGGGTGGTGG+TGG	0.381814	1:+56244872	None:intergenic
TCTTCTCTGTCTGTTGTAGT+TGG	0.385092	1:+56244159	None:intergenic
ATTTCAACAAGTCCTTCATT+TGG	0.388996	1:+56244857	None:intergenic
GTACCAACCATATAATTTCA+TGG	0.396015	1:-56244784	MsaT003406.1:CDS
TCTTGTGATTCTAGTGCCTC+AGG	0.405629	1:+56243924	None:intergenic
TGAATTTGGGGTGTCGGAGT+TGG	0.413715	1:-56244015	MsaT003406.1:CDS
TGTATCCCAAACCACAAAAC+TGG	0.420129	1:+56244748	None:intergenic
CAACAAGTCCTTCATTTGGT+TGG	0.420416	1:+56244861	None:intergenic
CCAACACAACCAACTTCAAT+AGG	0.420869	1:+56244377	None:intergenic
CTCGGTGAAAGAAGAATACT+TGG	0.431235	1:-56244906	MsaT003406.1:CDS
GACATCTATTGAGGAACATA+AGG	0.431334	1:-56244421	MsaT003406.1:CDS
CCTATTGAAGTTGGTTGTGT+TGG	0.439872	1:-56244377	MsaT003406.1:CDS
ATAGATGTCTTTGCCCTCTC+AGG	0.446863	1:+56244435	None:intergenic
CCTTCTCAGCCTATTGAAGT+TGG	0.448492	1:-56244386	MsaT003406.1:CDS
TGGGGTGGTGGTGGAATCGT+TGG	0.458015	1:+56244881	None:intergenic
GTATCTGGGAAGGAGATGAT+TGG	0.458621	1:+56244715	None:intergenic
ACAAGTCCTTCATTTGGTTG+GGG	0.464149	1:+56244863	None:intergenic
GGGGAAAATAATGGATAAAG+AGG	0.470921	1:-56243958	MsaT003406.1:CDS
AGTCCTTCATTTGGTTGGGG+TGG	0.486652	1:+56244866	None:intergenic
GTTCCATGAAATTATATGGT+TGG	0.492988	1:+56244781	None:intergenic
TGATTATGAATTTGGGGTGT+CGG	0.498346	1:-56244021	MsaT003406.1:CDS
GCTTGATGATGAAGGGTTCT+GGG	0.499647	1:-56243886	MsaT003406.1:CDS
AATGTTAGCTATGGAAATGC+AGG	0.499978	1:-56243991	MsaT003406.1:CDS
TTATTGTGTTTGAAGTATCT+GGG	0.502771	1:+56244701	None:intergenic
CAAGGAACTAGACTAGGTAT+TGG	0.504481	1:-56244083	MsaT003406.1:CDS
ATGTTTAGTACGAAATTTGA+GGG	0.507534	1:-56243855	MsaT003406.1:intron
GAGGAACATAAGGAGAAAGA+AGG	0.510760	1:-56244411	MsaT003406.1:CDS
AATGTTTAGTACGAAATTTG+AGG	0.531411	1:-56243856	MsaT003406.1:CDS
GGAGTTGGAAATGTTAGCTA+TGG	0.532242	1:-56244000	MsaT003406.1:CDS
ACTAGAATCACAAGAGAAGA+TGG	0.536908	1:-56243916	MsaT003406.1:CDS
CCACCACCCCAACCAAATGA+AGG	0.544676	1:-56244869	MsaT003406.1:CDS
TGTGTTTGAAGTATCTGGGA+AGG	0.547658	1:+56244705	None:intergenic
CAAATGAAAGATTCCTGAGA+GGG	0.550958	1:-56244448	MsaT003406.1:CDS
ATTACGTGATTATGAATTTG+GGG	0.552478	1:-56244027	MsaT003406.1:CDS
AGGAACATAAGGAGAAAGAA+GGG	0.559452	1:-56244410	MsaT003406.1:CDS
GAAAGAGTGCTTGATGATGA+AGG	0.560834	1:-56243894	MsaT003406.1:CDS
TGTAGTTGGTGAATGAAGAC+AGG	0.561937	1:+56244173	None:intergenic
AGATGGAACATAGGTTAGAA+GGG	0.564470	1:-56244250	MsaT003406.1:CDS
CACACTGTATCTGCTAGAGA+TGG	0.569897	1:-56244267	MsaT003406.1:CDS
CCAACTTCAATAGGCTGAGA+AGG	0.573783	1:+56244386	None:intergenic
AAGAGAAAGCTTGTTGAACA+AGG	0.580615	1:-56244101	MsaT003406.1:CDS
TCATAATCACGTAATTCTAA+CGG	0.586096	1:+56244035	None:intergenic
GGGAAGGAGATGATTGGAGA+AGG	0.595385	1:+56244721	None:intergenic
GAGAATGAATTATATGTACT+CGG	0.595766	1:-56244924	None:intergenic
GAGGGCAAAGACATCTATTG+AGG	0.598712	1:-56244430	MsaT003406.1:CDS
ATAATTTCATGGAACAGAAG+TGG	0.614186	1:-56244773	MsaT003406.1:CDS
GCAAATGAAAGATTCCTGAG+AGG	0.614961	1:-56244449	MsaT003406.1:CDS
AGAGAAACACGTGTTGATGT+CGG	0.625455	1:-56244324	MsaT003406.1:CDS
GTTGAACAAGGAACTAGACT+AGG	0.638387	1:-56244089	MsaT003406.1:CDS
ATCTGCTAGAGATGGAACAT+AGG	0.648151	1:-56244259	MsaT003406.1:CDS
AGAGGAAGTACATGAACCTG+AGG	0.670115	1:-56243940	MsaT003406.1:CDS
AAAGAGTGCTTGATGATGAA+GGG	0.670867	1:-56243893	MsaT003406.1:CDS
GTAGTTGGTGAATGAAGACA+GGG	0.704135	1:+56244174	None:intergenic
CATCTCTAGCAGATACAGTG+TGG	0.710812	1:+56244268	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	56242888	56244942	56242888	ID=MsaG003406
Chr1	mRNA	56242888	56244942	56242888	ID=MsaT003406.1;Parent=MsaG003406
Chr1	exon	56242888	56242910	56242888	ID=MsaT003406.1.exon3;Parent=MsaT003406.1
Chr1	CDS	56242888	56242910	56242888	ID=cds.MsaT003406.1;Parent=MsaT003406.1
Chr1	exon	56243856	56244507	56243856	ID=MsaT003406.1.exon2;Parent=MsaT003406.1
Chr1	CDS	56243856	56244507	56243856	ID=cds.MsaT003406.1;Parent=MsaT003406.1
Chr1	exon	56244667	56244942	56244667	ID=MsaT003406.1.exon1;Parent=MsaT003406.1
Chr1	CDS	56244667	56244942	56244667	ID=cds.MsaT003406.1;Parent=MsaT003406.1

Gene Sequence

>MsaT003406.1

ATGAATTATATGTACTCGGTGAAAGAAGAATACTTGGAACCAACGATTCCACCACCACCCCAACCAAATGAAGGACTTGTTGAAATTAGACCACAACCATTCCTCACAAAAACATTTGATGTAGTTGATGATCAAAGTACCAACCATATAATTTCATGGAACAGAAGTGGCACCAGTTTTGTGGTTTGGGATACACATGCCTTCTCCAATCATCTCCTTCCCAGATACTTCAAACACAATAATTTCTCAAGTTTTGTCAGACAATTAAACACTTATGGATTTAGAAAAATTGATCCAGATAGATGGGAATTTGCAAATGAAAGATTCCTGAGAGGGCAAAGACATCTATTGAGGAACATAAGGAGAAAGAAGGGACCTTCTCAGCCTATTGAAGTTGGTTGTGTTGGACTAGATGCAGAAATTGATCGTTTAAGGCAAGAGAAACACGTGTTGATGTCGGAGCTTGTGAGTCTTAGAAAGCAGCAATATGATACCACACTGTATCTGCTAGAGATGGAACATAGGTTAGAAGGGATAGAAATCAATCAGAAGAAAATGATGTCTTTCTTAGCTAGAGCTATGAAAAACCCTGTCTTCATTCACCAACTACAACAGACAGAGAAGAAAAAGGAGCTTGAAGAATCCGCGACCATGACAAAAAAGAGAAAGCTTGTTGAACAAGGAACTAGACTAGGTATTGGACAATCAAGCAGTGTTAAAGTTGAGCCGTTAGAATTACGTGATTATGAATTTGGGGTGTCGGAGTTGGAAATGTTAGCTATGGAAATGCAGGTTTTTGGAAGGGGGAAAATAATGGATAAAGAGGAAGTACATGAACCTGAGGCACTAGAATCACAAGAGAAGATGGAAAGAGTGCTTGATGATGAAGGGTTCTGGGAAGATCTAATGTTTAGTACGAAATTTGAGGTAAAGGGTGTTGCTTTGCTGTAG

Protein sequence

>MsaT003406.1

MNYMYSVKEEYLEPTIPPPPQPNEGLVEIRPQPFLTKTFDVVDDQSTNHIISWNRSGTSFVVWDTHAFSNHLLPRYFKHNNFSSFVRQLNTYGFRKIDPDRWEFANERFLRGQRHLLRNIRRKKGPSQPIEVGCVGLDAEIDRLRQEKHVLMSELVSLRKQQYDTTLYLLEMEHRLEGIEINQKKMMSFLARAMKNPVFIHQLQQTEKKKELEESATMTKKRKLVEQGTRLGIGQSSSVKVEPLELRDYEFGVSELEMLAMEMQVFGRGKIMDKEEVHEPEALESQEKMERVLDDEGFWEDLMFSTKFEVKGVALL*