AlfalfaGEDB | MsaG003428

M. sativa ssp. caerulea / MsaG003428

KEGG: -
GO: -
PFAM: PF04844.16

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003428	XP_003590633.1	95.417	240	8	2	1	240	1	237	2.21e-161	456

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003428	sp\|F4I8R6\|OFP12_ARATH	37.220	223	87	7	49	239	19	220	1.02e-26	105

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003428	tr\|G7IDP8\|G7IDP8_MEDTR	95.417	240	8	2	1	240	1	237	1.06e-161	456

TFs/TRs:

Gene ID	Type	Classification
MsaG003428	TF	OFP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG003428	MsaG006383	0.818320	3.635188e-52	7.650250e-50
MsaG003428	MsaG029521	0.800194	2.700392e-48	3.667010e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003428	MtrunA17_Chr1g0185481	95.417	240	8	2	1	240	33	269	7.09e-165	456
MsaG003428	MtrunA17_Chr7g0259991	51.452	241	58	10	6	238	12	201	2.76e-60	189
MsaG003428	MtrunA17_Chr1g0205341	47.101	138	50	3	102	237	63	179	1.11e-34	124
MsaG003428	MtrunA17_Chr2g0331871	38.636	132	69	3	118	237	142	273	1.21e-17	80.5
MsaG003428	MtrunA17_Chr7g0267101	40.157	127	52	5	111	237	97	199	1.55e-17	79.7
MsaG003428	MtrunA17_Chr4g0004341	50.649	77	35	2	159	234	175	249	8.03e-16	75.1
MsaG003428	MtrunA17_Chr1g0210351	51.429	70	29	2	166	235	135	199	6.45e-15	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003428	AT1G05420.1	37.220	223	87	7	49	239	19	220	1.04e-27	105
MsaG003428	AT1G05420.2	37.220	223	87	7	49	239	35	236	1.56e-27	105
MsaG003428	AT2G32100.1	37.423	163	72	5	95	239	88	238	7.70e-24	96.3
MsaG003428	AT4G14860.1	57.143	77	31	1	165	239	96	172	3.54e-22	90.5
MsaG003428	AT1G79960.1	45.946	74	40	0	164	237	187	260	3.46e-17	79.3
MsaG003428	AT2G36050.1	33.600	125	55	2	113	237	77	173	8.87e-13	66.6
MsaG003428	AT3G52540.1	31.200	125	58	3	113	237	104	200	1.47e-11	63.2

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACTGATAGTTGTAGAAAT+TGG	0.240910	1:-56732845	None:intergenic
TTCCTCAGATTTCTTGTGTT+TGG	0.279960	1:-56732767	None:intergenic
AACACTCACAAGTTCATTCT+TGG	0.302219	1:+56733424	MsaT003428.1:CDS
CTACTCACCTGATCCTTATA+TGG	0.303517	1:+56733285	MsaT003428.1:CDS
CTTCTCACGCTCAATACTAT+TGG	0.339069	1:-56733206	None:intergenic
AAATCTGCTGGCTCAGGTTC+GGG	0.339475	1:-56733064	None:intergenic
TAAAGTGTGGTCTGAGGTTA+AGG	0.350644	1:-56732981	None:intergenic
ATGAACTTGTGAGTGTTCTT+TGG	0.354503	1:-56733418	None:intergenic
GATCTTCTAAAATCCATATA+AGG	0.364006	1:-56733298	None:intergenic
TGATGGATGTGAAATCAAAT+TGG	0.370791	1:+56733353	MsaT003428.1:CDS
GAAATCTGCTGGCTCAGGTT+CGG	0.383556	1:-56733065	None:intergenic
AAGGCTGCTGCGAAATCTGC+TGG	0.394882	1:-56733076	None:intergenic
TGAACTTGTGAGTGTTCTTT+GGG	0.402029	1:-56733417	None:intergenic
TGTATATTCAATTAATGAGT+TGG	0.404911	1:-56733137	None:intergenic
GTTGTAGAAATTGGACTTGT+GGG	0.416852	1:-56732836	None:intergenic
CGCTTCTTCTTCTCCTCCCC+CGG	0.419956	1:+56733109	MsaT003428.1:CDS
GAAACAGGAGAAAGAGAAAG+AGG	0.423520	1:+56733228	MsaT003428.1:CDS
TATTGAGCGTGAGAAGAAAC+AGG	0.440175	1:+56733213	MsaT003428.1:CDS
AATTCTTGATCATAATTGAC+GGG	0.444957	1:-56732937	None:intergenic
GCTGCGAAATCTGCTGGCTC+AGG	0.454177	1:-56733070	None:intergenic
AGAGATGGTGGAGGCGCGAC+CGG	0.455026	1:+56733327	MsaT003428.1:CDS
CTAAAATCCATATAAGGATC+AGG	0.462583	1:-56733292	None:intergenic
AATTAATGAGTTGGAGCTAC+CGG	0.467305	1:-56733128	None:intergenic
ATACTATTGGACTTATCCAT+TGG	0.483511	1:-56733193	None:intergenic
GGAGGCGCGACCGGAGTTGA+TGG	0.488669	1:+56733336	MsaT003428.1:CDS
AGATTGCATTCAGCATTCTT+CGG	0.492875	1:+56733165	MsaT003428.1:CDS
TGTTCTTTGGGTTGAGAGCA+AGG	0.508816	1:-56733405	None:intergenic
GAAGAAGCGTTGAGAGGCGA+AGG	0.509416	1:-56733095	None:intergenic
ATTAATGAGTTGGAGCTACC+GGG	0.511814	1:-56733127	None:intergenic
CTATTGGACTTATCCATTGG+TGG	0.513854	1:-56733190	None:intergenic
TTCAACTTCGAGTTCAAAAG+AGG	0.515870	1:-56733041	None:intergenic
GCATTCTTCGGTACCACCAA+TGG	0.516823	1:+56733177	MsaT003428.1:CDS
AAAAGAGGTTGATGTAGTAA+AGG	0.520478	1:-56733026	None:intergenic
AAATTCTTGATCATAATTGA+CGG	0.524121	1:-56732938	None:intergenic
GATGCAGATGTAGATGAAGA+AGG	0.533865	1:-56732911	None:intergenic
AGAGGTTGATGTAGTAAAGG+TGG	0.557401	1:-56733023	None:intergenic
ACCATGACTAAAATGACATG+AGG	0.563547	1:-56733471	None:intergenic
CATATAAGGATCAGGTGAGT+AGG	0.566346	1:-56733284	None:intergenic
GTGTGGTCTGAGGTTAAGGA+AGG	0.576057	1:-56732977	None:intergenic
TAGAAGATCGATGCAAGAGA+TGG	0.580424	1:+56733312	MsaT003428.1:CDS
GATTTCACATCCATCAACTC+CGG	0.581756	1:-56733346	None:intergenic
AAGATCGATGCAAGAGATGG+TGG	0.583074	1:+56733315	MsaT003428.1:CDS
AGTTGTAGAAATTGGACTTG+TGG	0.600672	1:-56732837	None:intergenic
ATCGATGCAAGAGATGGTGG+AGG	0.602628	1:+56733318	MsaT003428.1:CDS
GGAGAAGAAGAAGCGTTGAG+AGG	0.620274	1:-56733101	None:intergenic
CATGAGGGTTACGAGAAGAT+CGG	0.623248	1:-56733455	None:intergenic
TTAATGAGTTGGAGCTACCG+GGG	0.639288	1:-56733126	None:intergenic
GTAGAAATTGGACTTGTGGG+TGG	0.648560	1:-56732833	None:intergenic
AGAGCATAAAGTGTGGTCTG+AGG	0.656840	1:-56732987	None:intergenic
TAAGGATCAGGTGAGTAGGT+CGG	0.659190	1:-56733280	None:intergenic
ATTCTTGATCATAATTGACG+GGG	0.668719	1:-56732936	None:intergenic
GTGAAGAAGAGCATAAAGTG+TGG	0.669604	1:-56732994	None:intergenic
CCATGACTAAAATGACATGA+GGG	0.681032	1:-56733470	None:intergenic
TGTGGTCTGAGGTTAAGGAA+GGG	0.682154	1:-56732976	None:intergenic
TTGATGATGATGTTGATGTG+AGG	0.685082	1:-56732876	None:intergenic
TAATGAGTTGGAGCTACCGG+GGG	0.686460	1:-56733125	None:intergenic
TGCCAAACACAAGAAATCTG+AGG	0.686722	1:+56732765	MsaT003428.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	56732764	56733486	56732764	ID=MsaG003428
Chr1	mRNA	56732764	56733486	56732764	ID=MsaT003428.1;Parent=MsaG003428
Chr1	exon	56732764	56733486	56732764	ID=MsaT003428.1.exon1;Parent=MsaT003428.1
Chr1	CDS	56732764	56733486	56732764	ID=cds.MsaT003428.1;Parent=MsaT003428.1

Gene Sequence

>MsaT003428.1

ATGCCAAACACAAGAAATCTGAGGAACCTTAACCTATGTTTTTCAAATTCAACTCTTGAATCAAAATCTCCACCCACAAGTCCAATTTCTACAACTATCAGTGAAGCAGCTTCCTCACATCAACATCATCATCAAATTCATCACAAACCTTCTTCATCTACATCTGCATCTTCCCCGTCAATTATGATCAAGAATTTCAACTCTCTCTATGACCCTTCCTTAACCTCAGACCACACTTTATGCTCTTCTTCACTCTCTACCACCTTTACTACATCAACCTCTTTTGAACTCGAAGTTGAACCCGAACCTGAGCCAGCAGATTTCGCAGCAGCCTTCGCCTCTCAACGCTTCTTCTTCTCCTCCCCCGGTAGCTCCAACTCATTAATTGAATATACAAACACAGATTGCATTCAGCATTCTTCGGTACCACCAATGGATAAGTCCAATAGTATTGAGCGTGAGAAGAAACAGGAGAAAGAGAAAGAGGAAGTTTTGTTCAACGGAAGTGTGGCAGTACCGACCTACTCACCTGATCCTTATATGGATTTTAGAAGATCGATGCAAGAGATGGTGGAGGCGCGACCGGAGTTGATGGATGTGAAATCAAATTGGAACATCTTGCATGAGTTGCTTCTTTGTTACCTTGCTCTCAACCCAAAGAACACTCACAAGTTCATTCTTGGTGCTTTTGCCGATCTTCTCGTAACCCTCATGTCATTTTAG

Protein sequence

>MsaT003428.1

MPNTRNLRNLNLCFSNSTLESKSPPTSPISTTISEAASSHQHHHQIHHKPSSSTSASSPSIMIKNFNSLYDPSLTSDHTLCSSSLSTTFTTSTSFELEVEPEPEPADFAAAFASQRFFFSSPGSSNSLIEYTNTDCIQHSSVPPMDKSNSIEREKKQEKEKEEVLFNGSVAVPTYSPDPYMDFRRSMQEMVEARPELMDVKSNWNILHELLLCYLALNPKNTHKFILGAFADLLVTLMSF*