AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003445

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003445 MtrunA17_Chr1g0185621 91.512 648 40 1 1 633 14 661 0.0 1169
MsaG003445 MtrunA17_Chr8g0386921 55.376 186 82 1 2 186 10 195 4.76e-65 224
MsaG003445 MtrunA17_Chr8g0386921 31.802 283 152 7 331 592 307 569 2.10e-26 114
MsaG003445 MtrunA17_Chr5g0411341 36.066 183 101 6 2 177 49 222 4.22e-25 110
MsaG003445 MtrunA17_Chr5g0411341 30.435 184 122 3 429 606 472 655 6.36e-17 84.7
MsaG003445 MtrunA17_Chr5g0411331 35.912 181 104 5 2 177 32 205 4.29e-25 105
MsaG003445 MtrunA17_Chr8g0366751 32.275 189 106 6 2 180 58 234 5.99e-21 97.4
MsaG003445 MtrunA17_Chr8g0366751 31.250 176 117 2 427 599 443 617 4.03e-18 88.6
MsaG003445 MtrunA17_Chr3g0131691 35.204 196 114 5 433 625 289 474 1.24e-20 95.5
MsaG003445 MtrunA17_Chr2g0280161 35.032 157 92 3 447 602 177 324 2.29e-19 90.1
MsaG003445 MtrunA17_Chr8g0346151 33.880 183 93 5 434 606 271 435 1.24e-18 89.0
MsaG003445 MtrunA17_Chr8g0346151 24.444 180 83 3 8 180 35 168 1.40e-14 76.6
MsaG003445 MtrunA17_Chr2g0307141 32.258 186 105 6 447 627 183 352 1.46e-18 87.8
MsaG003445 MtrunA17_Chr8g0346161 32.948 173 104 3 434 606 283 443 4.80e-18 87.4
MsaG003445 MtrunA17_Chr8g0346161 26.519 181 90 5 7 180 37 181 6.61e-17 84.0
MsaG003445 MtrunA17_Chr7g0225901 30.061 163 98 4 428 588 73 221 8.04e-18 83.6
MsaG003445 MtrunA17_Chr4g0033391 31.313 198 104 6 447 633 147 323 8.83e-18 85.1
MsaG003445 MtrunA17_Chr5g0400621 32.468 154 95 2 435 587 144 289 1.08e-17 84.7
MsaG003445 MtrunA17_Chr4g0033291 26.780 295 178 9 342 623 48 317 1.21e-17 84.7
MsaG003445 MtrunA17_Chr2g0307131 34.028 144 83 3 447 588 179 312 1.25e-17 85.1
MsaG003445 MtrunA17_Chr4g0054581 32.886 149 91 3 447 593 198 339 2.46e-17 84.3
MsaG003445 MtrunA17_Chr5g0400641 28.931 159 100 2 450 603 1 151 9.45e-17 78.2
MsaG003445 MtrunA17_Chr5g0400671 34.532 139 80 2 448 586 155 282 1.06e-16 81.6
MsaG003445 MtrunA17_Chr7g0252041 31.579 190 115 5 444 625 283 465 2.12e-16 82.4
MsaG003445 MtrunA17_Chr4g0049331 29.310 174 113 3 413 586 103 266 2.13e-16 80.5
MsaG003445 MtrunA17_Chr4g0002801 26.263 198 132 5 435 629 143 329 1.12e-15 78.6
MsaG003445 MtrunA17_Chr6g0468491 27.136 199 113 5 406 588 88 270 1.88e-15 77.8
MsaG003445 MtrunA17_Chr5g0400661 29.139 151 90 2 447 586 149 293 3.38e-15 77.4
MsaG003445 MtrunA17_Chr1g0211081 26.636 214 126 5 418 608 293 498 5.51e-15 78.2
MsaG003445 MtrunA17_Chr7g0260841 42.857 98 51 2 415 511 326 419 1.37e-14 76.6
MsaG003445 MtrunA17_Chr4g0002781 27.273 154 104 2 435 588 165 310 2.53e-14 74.7
MsaG003445 MtrunA17_Chr7g0275821 50.704 71 34 1 447 517 252 321 4.26e-14 74.7
MsaG003445 MtrunA17_Chr2g0333621 27.545 167 103 4 429 588 117 272 1.21e-13 72.4
MsaG003445 MtrunA17_Chr4g0054551 61.111 54 21 0 447 500 155 208 1.21e-13 72.4
MsaG003445 MtrunA17_Chr6g0468461 28.497 193 108 5 404 588 122 292 2.33e-13 72.0
MsaG003445 MtrunA17_Chr4g0054571 47.541 61 32 0 447 507 150 210 3.08e-13 71.2
MsaG003445 MtrunA17_Chr4g0054621 26.630 184 107 5 447 625 172 332 3.91e-13 71.2
MsaG003445 MtrunA17_Chr4g0054561 55.556 54 24 0 447 500 155 208 7.59e-13 70.1
MsaG003445 MtrunA17_Chr4g0033281 31.944 144 82 3 447 588 153 282 8.68e-13 70.1
MsaG003445 MtrunA17_Chr2g0333641 25.000 204 137 5 429 629 116 306 8.76e-13 69.7
MsaG003445 MtrunA17_Chr4g0052821 30.723 166 84 5 435 586 283 431 1.77e-12 70.1
MsaG003445 MtrunA17_Chr5g0411541 30.348 201 113 4 426 625 140 314 3.87e-12 67.8
MsaG003445 MtrunA17_Chr0c01g0489461 28.387 155 96 4 448 587 197 351 4.42e-12 68.6
MsaG003445 MtrunA17_Chr3g0141091 32.544 169 84 6 421 586 149 290 7.21e-12 67.4
MsaG003445 MtrunA17_Chr5g0426851 25.641 195 123 8 448 628 102 288 7.26e-12 66.6
MsaG003445 MtrunA17_Chr4g0052841 30.435 161 92 5 436 586 283 433 7.92e-12 68.2
MsaG003445 MtrunA17_Chr2g0333611 29.577 142 84 4 448 586 46 174 1.23e-11 64.7
MsaG003445 MtrunA17_Chr8g0336681 26.543 162 95 4 434 586 148 294 2.15e-11 65.9
MsaG003445 MtrunA17_Chr1g0153241 51.786 56 27 0 448 503 96 151 3.42e-11 64.3
MsaG003445 MtrunA17_Chr2g0298751 38.202 89 48 1 404 492 16 97 5.39e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003445 AT4G09820.1 73.096 197 52 1 1 197 23 218 1.52e-102 322
MsaG003445 AT1G63650.3 32.722 654 335 21 2 628 17 592 6.32e-95 304
MsaG003445 AT1G63650.1 32.722 654 335 21 2 628 17 592 6.32e-95 304
MsaG003445 AT1G63650.2 32.722 654 335 21 2 628 17 592 6.32e-95 304
MsaG003445 AT5G41315.1 32.635 668 357 18 2 628 18 633 1.74e-94 304
MsaG003445 AT5G41315.2 32.635 668 357 18 2 628 26 641 1.83e-94 304
MsaG003445 AT5G41315.3 32.635 668 357 18 2 628 23 638 2.21e-94 304
MsaG003445 AT4G00480.3 40.816 196 98 4 2 182 27 219 7.75e-41 156
MsaG003445 AT4G00480.3 28.082 292 167 11 329 606 241 503 3.86e-17 85.1
MsaG003445 AT4G00480.1 40.816 196 98 4 2 182 27 219 1.29e-40 155
MsaG003445 AT4G00480.1 26.984 315 183 12 329 629 241 522 2.94e-17 85.9
MsaG003445 AT4G00480.2 40.816 196 98 4 2 182 27 219 5.91e-40 154
MsaG003445 AT4G00480.2 26.984 315 183 12 329 629 241 522 5.43e-17 85.1
MsaG003445 AT1G32640.1 31.746 189 111 5 2 180 72 252 1.30e-24 109
MsaG003445 AT1G32640.1 33.951 162 96 3 447 606 452 604 1.43e-17 87.0
MsaG003445 AT4G17880.1 30.366 191 114 4 2 180 67 250 2.02e-24 108
MsaG003445 AT4G17880.1 34.254 181 108 4 429 606 395 567 2.74e-19 92.4
MsaG003445 AT1G01260.1 35.263 190 108 6 11 193 66 247 4.08e-23 104
MsaG003445 AT1G01260.1 53.333 60 28 0 447 506 433 492 3.26e-13 73.2
MsaG003445 AT1G01260.2 35.263 190 108 6 11 193 66 247 4.08e-23 104
MsaG003445 AT1G01260.2 53.333 60 28 0 447 506 433 492 3.26e-13 73.2
MsaG003445 AT1G01260.3 35.263 190 108 6 11 193 66 247 4.08e-23 104
MsaG003445 AT1G01260.3 53.333 60 28 0 447 506 433 492 3.26e-13 73.2
MsaG003445 AT1G12860.2 37.195 164 99 2 444 606 264 424 2.69e-22 100
MsaG003445 AT5G46760.1 31.053 190 102 5 2 176 56 231 7.81e-22 100
MsaG003445 AT5G46760.1 33.750 160 102 2 447 606 415 570 2.30e-18 89.4
MsaG003445 AT1G12860.1 36.810 163 99 2 444 605 264 423 1.61e-21 98.2
MsaG003445 AT2G46510.1 30.366 191 117 4 7 187 62 246 1.37e-20 96.3
MsaG003445 AT2G46510.1 62.963 54 20 0 447 500 395 448 1.15e-14 77.8
MsaG003445 AT5G46830.1 28.571 182 113 4 11 180 49 225 3.55e-19 91.7
MsaG003445 AT5G46830.1 33.742 163 92 6 447 606 343 492 1.95e-12 70.5
MsaG003445 AT3G26744.6 36.145 166 102 3 444 606 304 468 9.40e-19 90.1
MsaG003445 AT3G26744.1 35.294 170 106 3 444 610 304 472 2.70e-18 89.0
MsaG003445 AT3G26744.4 35.294 170 106 3 444 610 304 472 2.70e-18 89.0
MsaG003445 AT3G26744.2 35.294 170 106 3 444 610 304 472 2.70e-18 89.0
MsaG003445 AT4G37850.2 33.133 166 91 5 435 596 145 294 5.90e-17 82.8
MsaG003445 AT4G37850.1 33.133 166 91 5 435 596 145 294 5.90e-17 82.8
MsaG003445 AT2G22760.2 33.533 167 89 5 433 585 96 254 1.14e-14 75.5
MsaG003445 AT2G22760.1 33.533 167 89 5 433 585 96 254 2.43e-14 74.7
MsaG003445 AT2G16910.1 45.122 82 39 1 425 500 286 367 1.18e-13 74.3
MsaG003445 AT4G16430.1 29.730 185 112 5 2 180 54 226 1.83e-13 73.6
MsaG003445 AT4G16430.1 41.748 103 56 3 410 512 286 384 1.90e-13 73.6
MsaG003445 AT4G00870.1 52.459 61 29 0 447 507 249 309 4.60e-13 72.0
MsaG003445 AT4G38070.1 30.405 148 86 4 416 546 54 201 4.73e-13 70.1
MsaG003445 AT2G22770.1 28.402 169 114 2 426 587 111 279 5.79e-13 70.9
MsaG003445 AT1G72210.1 26.842 190 125 5 448 629 127 310 6.95e-13 70.5
MsaG003445 AT5G57150.5 32.812 128 65 3 415 537 38 149 2.52e-12 67.4
MsaG003445 AT5G57150.3 32.812 128 65 3 415 537 39 150 2.78e-12 67.0
MsaG003445 AT5G57150.2 32.812 128 65 3 415 537 39 150 3.04e-12 67.0
MsaG003445 AT5G57150.1 32.812 128 65 3 415 537 38 149 3.60e-12 67.4
MsaG003445 AT2G22750.4 38.384 99 48 3 411 496 105 203 4.72e-12 68.2
MsaG003445 AT2G22750.3 38.384 99 48 3 411 496 105 203 4.80e-12 68.2
MsaG003445 AT2G22750.1 38.384 99 48 3 411 496 77 175 4.88e-12 67.8
MsaG003445 AT2G22750.5 38.384 99 48 3 411 496 101 199 4.95e-12 68.2
MsaG003445 AT2G22750.2 38.384 99 48 3 411 496 77 175 5.25e-12 67.8
MsaG003445 AT4G29930.4 32.667 150 82 6 426 567 35 173 5.94e-12 65.5
MsaG003445 AT5G57150.4 32.812 128 65 3 415 537 39 150 7.98e-12 66.6
MsaG003445 AT4G29930.2 39.759 83 48 1 426 508 35 115 9.43e-12 65.5
MsaG003445 AT4G29930.6 29.630 162 99 4 426 587 35 181 1.04e-11 65.5
MsaG003445 AT4G29930.1 37.383 107 59 3 426 532 35 133 1.41e-11 65.9
MsaG003445 AT4G29930.5 39.759 83 48 1 426 508 35 115 1.75e-11 65.1
MsaG003445 AT4G29930.3 39.759 83 48 1 426 508 35 115 2.56e-11 65.1
MsaG003445 AT4G29930.7 39.759 83 48 1 426 508 35 115 2.56e-11 65.1
MsaG003445 AT3G61950.6 29.730 148 84 4 447 587 128 262 7.02e-11 64.3

Find 176 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGTTGTGAGGTATAATT+TGG 0.209014 1:+56931770 None:intergenic
CTCTTTGTGTATGCCCTTCC+TGG 0.213816 1:+56935796 None:intergenic
TCACGTGTTTGATGAAATTA+AGG 0.245342 1:+56934977 None:intergenic
GAGGTGGTGGAGGTTGTTTC+CGG 0.247905 1:+56932480 None:intergenic
ATGGACAAAGCTTCTATATT+AGG 0.273239 1:-56932222 MsaT003445.1:CDS
GGTTCCATTCGTGACTAAAA+TGG 0.273619 1:-56932241 MsaT003445.1:CDS
TTCATCCATGTCGTCTTGAT+TGG 0.294810 1:+56934819 None:intergenic
TTGAAATGCCCGATGATATT+CGG 0.302845 1:-56934663 MsaT003445.1:CDS
TGATTTGATATACAGGATTC+TGG 0.312781 1:-56939341 MsaT003445.1:intron
CATAGAGTTCTCTTAGTTGC+TGG 0.317996 1:+56939224 None:intergenic
GAGTTGAGGATTGAGGTTAT+TGG 0.318064 1:-56931907 MsaT003445.1:CDS
GTTCAATCTTCTCTTAATAA+TGG 0.319413 1:-56931883 MsaT003445.1:CDS
TAGAAGTGGTGTAACGGTTT+TGG 0.322614 1:-56932112 MsaT003445.1:CDS
AGGATTGTTGCTTGATGTTA+TGG 0.331605 1:-56931941 MsaT003445.1:CDS
GTGAAGGAAAATGGTGGAAA+TGG 0.337697 1:-56931832 MsaT003445.1:CDS
AATTATTTGACCCGTCGTTT+GGG 0.340247 1:+56934632 None:intergenic
AAATTATTTGACCCGTCGTT+TGG 0.342913 1:+56934631 None:intergenic
GTGGAGGTTGTTTCCGGTGG+TGG 0.345072 1:+56932486 None:intergenic
TTACCCACGGGTTCTTCAAT+TGG 0.352267 1:+56934514 None:intergenic
GGAGGTTGCTGCTTCGGTTC+AGG 0.361607 1:-56932016 MsaT003445.1:CDS
GGTTGAGGAAGTTGGATTAG+AGG 0.367769 1:+56934894 None:intergenic
GAGGCATTGGGAGGTGGTGG+AGG 0.370793 1:+56932470 None:intergenic
CCCAATCCTCTTTCACCAAC+TGG 0.373009 1:-56932536 MsaT003445.1:CDS
TGAAATGCCCGATGATATTC+GGG 0.373074 1:-56934662 MsaT003445.1:CDS
TGAGACGGAGGTTGCTGCTT+CGG 0.382360 1:-56932022 MsaT003445.1:CDS
TTTGAGGAGGCATTGGGAGG+TGG 0.387314 1:+56932464 None:intergenic
TGGGGTGATGGATATTATAA+TGG 0.391029 1:-56939316 MsaT003445.1:CDS
AGAACCATTCAGATTCTGTT+AGG 0.392300 1:+56939134 None:intergenic
AGGGCTAAGGTGAAGGAAAA+TGG 0.395483 1:-56931841 MsaT003445.1:CDS
TGTTCCTTTGCCGCGTAACT+TGG 0.398145 1:+56932353 None:intergenic
TCATCCATGTCGTCTTGATT+GGG 0.400362 1:+56934820 None:intergenic
ATGTGATCGGTTGAGGAAGT+TGG 0.404252 1:+56934886 None:intergenic
GGAGTAAGTGATGTAGTTGA+TGG 0.405895 1:+56932560 None:intergenic
TAAGCTTCAAAACATGTTGC+AGG 0.406229 1:-56940272 None:intergenic
TTACTAGTTGTGAGTAATCA+AGG 0.407904 1:-56934592 MsaT003445.1:CDS
CAAGTTCAACTACCATCTTC+AGG 0.412992 1:-56934484 MsaT003445.1:intron
TCAGATTCTGTTAGGTCCTC+AGG 0.415594 1:+56939142 None:intergenic
GTGGTGGCAGCGCGTGGAAG+TGG 0.418946 1:+56932502 None:intergenic
GGATGTATTTGAGGAGGCAT+TGG 0.421906 1:+56932457 None:intergenic
GTTGAGGGAGTTGAGGATTG+AGG 0.424843 1:-56931914 MsaT003445.1:CDS
TGTTCTGAGAGTGCTGGCTT+TGG 0.425999 1:+56934922 None:intergenic
TATTGAGAGTGATGCTTTGT+TGG 0.427384 1:-56931986 MsaT003445.1:CDS
AACCCATCTTTGTGTTGAAT+CGG 0.430063 1:+56934537 None:intergenic
CTACCTGCATCATGGGTTTG+AGG 0.432030 1:+56932387 None:intergenic
TGAGTTTGGCACAACGGATA+AGG 0.438119 1:-56935347 MsaT003445.1:intron
TTGGATGGCGTAGTTGAGTT+TGG 0.438891 1:-56935361 MsaT003445.1:CDS
TCCTCTGCACTAACCTCCAT+TGG 0.444642 1:+56939265 None:intergenic
TGATATACAGGATTCTGGTA+TGG 0.452389 1:-56939336 MsaT003445.1:intron
TCCTTTGCCGCGTAACTTGG+CGG 0.452672 1:+56932356 None:intergenic
GAGGAGGCATTGGGAGGTGG+TGG 0.453489 1:+56932467 None:intergenic
AGTGGACTTATAGCCTCTTC+TGG 0.455737 1:-56940231 MsaT003445.1:CDS
ACTATTAATTTACCTGAAGA+TGG 0.457762 1:+56934472 None:intergenic
CCAGTTGGTGAAAGAGGATT+GGG 0.458813 1:+56932536 None:intergenic
GCAGCGCGTGGAAGTGGTGG+TGG 0.460378 1:+56932508 None:intergenic
TCTGAGAGTGCTGGCTTTGG+CGG 0.460852 1:+56934925 None:intergenic
TGGTAATGTTGAGGGAGTTG+AGG 0.465496 1:-56931921 MsaT003445.1:CDS
GGGTCCAACAGAGAAGAAAA+AGG 0.466081 1:-56932088 MsaT003445.1:CDS
TCCAGTTGGTGAAAGAGGAT+TGG 0.466348 1:+56932535 None:intergenic
CTGTGAAGAGGATGTATTTG+AGG 0.466470 1:+56932448 None:intergenic
GTGGTGGTGGTTGGTCCAGT+TGG 0.466928 1:+56932521 None:intergenic
GATTGGGATTTGTAGAGGGT+GGG 0.467067 1:+56934836 None:intergenic
TGAAGGAAAATGGTGGAAAT+GGG 0.468007 1:-56931831 MsaT003445.1:CDS
GTTGGTCCAGTTGGTGAAAG+AGG 0.468465 1:+56932530 None:intergenic
TTGCCGCGTAACTTGGCGGA+GGG 0.469002 1:+56932360 None:intergenic
TTCAAGAGCTATTCTAGCTA+AGG 0.470826 1:-56935725 MsaT003445.1:intron
TACCTGCATCATGGGTTTGA+GGG 0.471392 1:+56932388 None:intergenic
ATTGAGTGTTTGCATAGAGA+AGG 0.471413 1:-56931961 MsaT003445.1:CDS
GATGTATTTGAGGAGGCATT+GGG 0.472065 1:+56932458 None:intergenic
TGTGGTATGCATTCCTGTAT+TGG 0.472277 1:-56935380 MsaT003445.1:CDS
CAATGTTCCATTGTGTTGAC+TGG 0.472479 1:+56932595 None:intergenic
GATATACAGGATTCTGGTAT+GGG 0.472998 1:-56939335 MsaT003445.1:intron
ACACAAAGAGACAGCATGTA+TGG 0.473379 1:-56935783 MsaT003445.1:CDS
TGGCTAGAGTGCTAATATAC+AGG 0.479087 1:-56935536 MsaT003445.1:intron
TTCTTCGCTCCGCGAGGACG+TGG 0.479679 1:+56932307 None:intergenic
TGCATCATGGGTTTGAGGGG+AGG 0.480084 1:+56932392 None:intergenic
TGATTGGGATTTGTAGAGGG+TGG 0.486223 1:+56934835 None:intergenic
AAACCGATTCAACACAAAGA+TGG 0.487993 1:-56934540 MsaT003445.1:CDS
TGCCCGATGATATTCGGGTT+GGG 0.493884 1:-56934657 MsaT003445.1:CDS
GTGGCAGCGCGTGGAAGTGG+TGG 0.496583 1:+56932505 None:intergenic
TCAACTACGCCATCCAATAC+AGG 0.497764 1:+56935367 None:intergenic
CTTCCACGCGCTGCCACCAC+CGG 0.497913 1:-56932499 MsaT003445.1:CDS
TATATATAAGGTTGCCAGGA+AGG 0.500355 1:-56935810 MsaT003445.1:intron
AGGGGTCACTACCTGCATCA+TGG 0.501217 1:+56932379 None:intergenic
CTCTATGAATCATTGTCTGC+CGG 0.501432 1:-56939208 MsaT003445.1:CDS
AGGATTCTGGTATGGGGTGA+TGG 0.503669 1:-56939328 MsaT003445.1:CDS
TTTCCGGTGGTGGCAGCGCG+TGG 0.505786 1:+56932496 None:intergenic
GCACTATATATAAGGTTGCC+AGG 0.507294 1:-56935814 MsaT003445.1:intron
GGTAGGAATGTGATCGGTTG+AGG 0.510024 1:+56934879 None:intergenic
CTCAACTGCTTTCGCTCTCG+TGG 0.513238 1:+56932041 None:intergenic
TCCCCTCAAACCCATGATGC+AGG 0.514528 1:-56932390 MsaT003445.1:CDS
TCGCTCCGCGAGGACGTGGT+TGG 0.516505 1:+56932311 None:intergenic
GGGGTCACTACCTGCATCAT+GGG 0.516990 1:+56932380 None:intergenic
TGTTGCGGAGTTGAGGGCTA+AGG 0.522771 1:-56931854 MsaT003445.1:CDS
GTTGTTCAGTCTCTATCTGA+CGG 0.523038 1:+56932139 None:intergenic
CGCGTGGAAGTGGTGGTGGT+TGG 0.524425 1:+56932512 None:intergenic
CATGGATGAAGACGAGGAAG+AGG 0.525017 1:-56934806 MsaT003445.1:CDS
GTTGAGTGTTCTGAGAGTGC+TGG 0.525057 1:+56934916 None:intergenic
TCCGCCAAGTTACGCGGCAA+AGG 0.528253 1:-56932357 MsaT003445.1:CDS
TGATGTTATGGTAATGTTGA+GGG 0.530343 1:-56931929 MsaT003445.1:CDS
AGTTGGTGGATTTGTCTCTC+CGG 0.531556 1:+56939189 None:intergenic
GTGGTGGAGGTTGTTTCCGG+TGG 0.532605 1:+56932483 None:intergenic
TACGAGTCCACGTGTCCTGA+CGG 0.533941 1:+56934565 None:intergenic
GACCCAACCCGAATATCATC+GGG 0.537575 1:+56934655 None:intergenic
GCACATGGACGACGAGTTGG+TGG 0.545968 1:+56939175 None:intergenic
AGTAATCAAGGAAACCCGTC+AGG 0.550819 1:-56934580 MsaT003445.1:CDS
AACCGATTCAACACAAAGAT+GGG 0.550895 1:-56934539 MsaT003445.1:CDS
GCTTTGGCGGTAAAGAGTGT+TGG 0.551248 1:+56934938 None:intergenic
GGACCCAACCCGAATATCAT+CGG 0.555510 1:+56934654 None:intergenic
TCTTGATTGGGATTTGTAGA+GGG 0.555864 1:+56934832 None:intergenic
GAGAGCGAAAGCAGTTGAGA+CGG 0.556085 1:-56932037 MsaT003445.1:CDS
ATGCCCGATGATATTCGGGT+TGG 0.557418 1:-56934658 MsaT003445.1:CDS
ACGAGTCCACGTGTCCTGAC+GGG 0.558448 1:+56934566 None:intergenic
AGCGAAAGCAGTTGAGACGG+AGG 0.558576 1:-56932034 MsaT003445.1:CDS
TTTGCCGCGTAACTTGGCGG+AGG 0.559864 1:+56932359 None:intergenic
AGGACCTAACAGAATCTGAA+TGG 0.560680 1:-56939138 MsaT003445.1:CDS
ACTGAACAACAAAGTAGAAG+TGG 0.562802 1:-56932126 MsaT003445.1:CDS
GAAGCACATGGACGACGAGT+TGG 0.564910 1:+56939172 None:intergenic
AGACAGCATGTATGGCTCAC+GGG 0.565294 1:-56935775 MsaT003445.1:CDS
GGGTTGGGTCCCCAAACGAC+GGG 0.567731 1:-56934642 MsaT003445.1:CDS
ATATATAAGGTTGCCAGGAA+GGG 0.568067 1:-56935809 MsaT003445.1:intron
TGCCGCGTAACTTGGCGGAG+GGG 0.569500 1:+56932361 None:intergenic
GTGAGGATTGTTGAAGAATG+TGG 0.571926 1:-56932066 MsaT003445.1:CDS
TTGGCGCTCAGCTCGTCTTG+AGG 0.573080 1:+56932330 None:intergenic
AACTTGTAGCGAATTACCCA+CGG 0.574291 1:+56934501 None:intergenic
ACCTGCATCATGGGTTTGAG+GGG 0.574484 1:+56932389 None:intergenic
ATGTGCTTCCTTGTCACCTG+AGG 0.574896 1:-56939158 MsaT003445.1:CDS
GTATTTGAGGAGGCATTGGG+AGG 0.574965 1:+56932461 None:intergenic
TTGATGTTATGGTAATGTTG+AGG 0.575487 1:-56931930 MsaT003445.1:CDS
GTCTTGATTGGGATTTGTAG+AGG 0.577629 1:+56934831 None:intergenic
TGGGAAGAAAGTTAGTATTG+TGG 0.581181 1:-56931812 MsaT003445.1:CDS
GGAGTTGAGGGCTAAGGTGA+AGG 0.582755 1:-56931848 MsaT003445.1:CDS
TGTAGAGGGTGGGTCTGCCA+TGG 0.583492 1:+56934846 None:intergenic
CGGGTCAAATAATTTAGACT+CGG 0.585244 1:-56934623 MsaT003445.1:CDS
ATGATGACGTACAGTTGTTG+TGG 0.586185 1:+56940199 None:intergenic
GAAGAGAGAAACTCAATGAG+AGG 0.590853 1:-56932284 MsaT003445.1:CDS
CTCTTCTTCTTCGCTCCGCG+AGG 0.591427 1:+56932301 None:intergenic
GTCTGCCATGGTGTACATGA+TGG 0.591460 1:+56934858 None:intergenic
GACCCCTCCGCCAAGTTACG+CGG 0.591527 1:-56932363 MsaT003445.1:CDS
GAGACAGCATGTATGGCTCA+CGG 0.591616 1:-56935776 MsaT003445.1:CDS
CGGGTTGGGTCCCCAAACGA+CGG 0.593444 1:-56934643 MsaT003445.1:CDS
CTGCAGTGCAATCTGTTCAG+TGG 0.594326 1:-56940249 MsaT003445.1:CDS
ATTATTTGACCCGTCGTTTG+GGG 0.598865 1:+56934633 None:intergenic
TGATGACGTACAGTTGTTGT+GGG 0.599654 1:+56940200 None:intergenic
CGTAGTTGAGTTTGGCACAA+CGG 0.601213 1:-56935353 MsaT003445.1:CDS
TGTTAGGTCCTCAGGTGACA+AGG 0.602958 1:+56939150 None:intergenic
GTGGGCAAAGTTGCCAGAAG+AGG 0.606474 1:+56940218 None:intergenic
ACAAAGTAGAAGTGGTGTAA+CGG 0.607690 1:-56932118 MsaT003445.1:CDS
GGTTCCAATTGAAGAACCCG+TGG 0.613679 1:-56934518 MsaT003445.1:CDS
AGGAAACCCGTCAGGACACG+TGG 0.614112 1:-56934572 MsaT003445.1:CDS
TCGAAAGACAGTGCAACCAA+TGG 0.615013 1:-56939281 MsaT003445.1:CDS
TAAGTGATGTAGTTGATGGA+AGG 0.615344 1:+56932564 None:intergenic
ACCAATGGAGGTTAGTGCAG+AGG 0.616075 1:-56939266 MsaT003445.1:CDS
AAAGACAGTGCAACCAATGG+AGG 0.619772 1:-56939278 MsaT003445.1:CDS
ATGGCTCACGGGTGCAAATG+AGG 0.624999 1:-56935764 MsaT003445.1:CDS
GTATGCATTCCTGTATTGGA+TGG 0.632942 1:-56935376 MsaT003445.1:CDS
TGAGTTACTTACCCTACACC+AGG 0.633728 1:+56939082 None:intergenic
TTCAAAACCAGTCAACACAA+TGG 0.637914 1:-56932602 MsaT003445.1:CDS
AAATCCCAATCAAGACGACA+TGG 0.638757 1:-56934824 MsaT003445.1:CDS
GCCATGGTGTACATGATGGT+AGG 0.641072 1:+56934862 None:intergenic
GCTAAGGTGAAGGAAAATGG+TGG 0.644258 1:-56931838 MsaT003445.1:CDS
TCAGGTGACAAGGAAGCACA+TGG 0.644950 1:+56939160 None:intergenic
GGAGGTATGGGACTGTGAAG+AGG 0.648050 1:+56932436 None:intergenic
ATATACAGGATTCTGGTATG+GGG 0.653259 1:-56939334 MsaT003445.1:intron
TGAAGAGGATGTATTTGAGG+AGG 0.655583 1:+56932451 None:intergenic
AAAATGCACTAAATGTTGTG+AGG 0.655990 1:+56931760 None:intergenic
GCTCACGGGTGCAAATGAGG+TGG 0.662470 1:-56935761 MsaT003445.1:CDS
GTTGGTGAAAGAGGATTGGG+AGG 0.663743 1:+56932539 None:intergenic
ACTTGTAGCGAATTACCCAC+GGG 0.667266 1:+56934502 None:intergenic
TTTGAGGGGAGGTTTCGTCG+TGG 0.671898 1:+56932403 None:intergenic
GAGCGCCAACCACGTCCTCG+CGG 0.675341 1:-56932316 MsaT003445.1:CDS
CATGATGGTAGGAATGTGAT+CGG 0.682068 1:+56934873 None:intergenic
AGACGACATGGATGAAGACG+AGG 0.694697 1:-56934812 MsaT003445.1:CDS
AAGACGAAGCATTTAAACAG+AGG 0.697175 1:-56934744 MsaT003445.1:intron
GTTCCAATTGAAGAACCCGT+GGG 0.703614 1:-56934517 MsaT003445.1:CDS
TCCTACCATCATGTACACCA+TGG 0.714693 1:-56934863 MsaT003445.1:CDS
GTTACTTACCCTACACCAGG+AGG 0.750268 1:+56939085 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 56931768 56940281 56931768 ID=MsaG003445
Chr1 mRNA 56931768 56940281 56931768 ID=MsaT003445.1;Parent=MsaG003445
Chr1 exon 56931768 56932693 56931768 ID=MsaT003445.1.exon8;Parent=MsaT003445.1
Chr1 CDS 56931768 56932693 56931768 ID=cds.MsaT003445.1;Parent=MsaT003445.1
Chr1 exon 56934485 56934699 56934485 ID=MsaT003445.1.exon7;Parent=MsaT003445.1
Chr1 CDS 56934485 56934699 56934485 ID=cds.MsaT003445.1;Parent=MsaT003445.1
Chr1 exon 56934745 56935010 56934745 ID=MsaT003445.1.exon6;Parent=MsaT003445.1
Chr1 CDS 56934745 56935010 56934745 ID=cds.MsaT003445.1;Parent=MsaT003445.1
Chr1 exon 56935348 56935404 56935348 ID=MsaT003445.1.exon5;Parent=MsaT003445.1
Chr1 CDS 56935348 56935404 56935348 ID=cds.MsaT003445.1;Parent=MsaT003445.1
Chr1 exon 56935537 56935551 56935537 ID=MsaT003445.1.exon4;Parent=MsaT003445.1
Chr1 CDS 56935537 56935551 56935537 ID=cds.MsaT003445.1;Parent=MsaT003445.1
Chr1 exon 56935726 56935822 56935726 ID=MsaT003445.1.exon3;Parent=MsaT003445.1
Chr1 CDS 56935726 56935822 56935726 ID=cds.MsaT003445.1;Parent=MsaT003445.1
Chr1 exon 56939094 56939348 56939094 ID=MsaT003445.1.exon2;Parent=MsaT003445.1
Chr1 CDS 56939094 56939348 56939094 ID=cds.MsaT003445.1;Parent=MsaT003445.1
Chr1 exon 56940211 56940281 56940211 ID=MsaT003445.1.exon1;Parent=MsaT003445.1
Chr1 CDS 56940211 56940281 56940211 ID=cds.MsaT003445.1;Parent=MsaT003445.1
Gene Sequence

>MsaT003445.1

ATGTTGCAGGCTGCAGTGCAATCTGTTCAGTGGACTTATAGCCTCTTCTGGCAACTTTGCCCACAACAACTGATTCTGGTATGGGGTGATGGATATTATAATGGATCAATTAAGACTCGAAAGACAGTGCAACCAATGGAGGTTAGTGCAGAGGAAGCATCTCTACAAAGAAGCCAGCAACTAAGAGAACTCTATGAATCATTGTCTGCCGGAGAGACAAATCCACCAACTCGTCGTCCATGTGCTTCCTTGTCACCTGAGGACCTAACAGAATCTGAATGGTTCTATTTGATGTGTGTCTCTTTCTCTTTTCCTCCTGGTGTAGGGTTGCCAGGAAGGGCATACACAAAGAGACAGCATGTATGGCTCACGGGTGCAAATGAGGTGGATAGCAAAACATTTTCAAGAGCTATTCTAGCTAAGAGTGCTAATATACAGACTGTGGTATGCATTCCTGTATTGGATGGCGTAGTTGAGTTTGGCACAACGGATAAGATTCAAGAAGACCTTAATTTCATCAAACACGTGAAGAGCTTCTTTCTCGACCAACACTCTTTACCGCCAAAGCCAGCACTCTCAGAACACTCAACCTCTAATCCAACTTCCTCAACCGATCACATTCCTACCATCATGTACACCATGGCAGACCCACCCTCTACAAATCCCAATCAAGACGACATGGATGAAGACGAGGAAGAGGAAGAAGATGAAGAAGACGATGAAGTCGAATCAGAATCTGAAGACGAAGCATTTAAACAGAGTGAGTTGATGCAGATTGAAATGCCCGATGATATTCGGGTTGGGTCCCCAAACGACGGGTCAAATAATTTAGACTCGGATTTTCATTTACTAGTTGTGAGTAATCAAGGAAACCCGTCAGGACACGTGGACTCGTACAAAACCGATTCAACACAAAGATGGGTTCCAATTGAAGAACCCGTGGGTAATTCGCTACAAGTTCAACTACCATCTTCAGTACTTCATCATCAACTAGAAGACTTAACACAAGAAGACACACATTACTCTCAAACAGTATCAACCATCATTCAAAACCAGTCAACACAATGGAACATTGATTTTTCACCTTCCATCAACTACATCACTTACTCCTCCCAATCCTCTTTCACCAACTGGACCAACCACCACCACTTCCACGCGCTGCCACCACCGGAAACAACCTCCACCACCTCCCAATGCCTCCTCAAATACATCCTCTTCACAGTCCCATACCTCCACACCAAAAACCACGACGAAACCTCCCCTCAAACCCATGATGCAGGTAGTGACCCCTCCGCCAAGTTACGCGGCAAAGGAACACCTCAAGACGAGCTGAGCGCCAACCACGTCCTCGCGGAGCGAAGAAGAAGAGAGAAACTCAATGAGAGGTTCATTATTTTAAGATCATTGGTTCCATTCGTGACTAAAATGGACAAAGCTTCTATATTAGGTGACACTATTGAGTACTTGAAACAGCTAAGAAGAAAGATACAAGATCTTGAAACACGTAACCGTCAGATAGAGACTGAACAACAAAGTAGAAGTGGTGTAACGGTTTTGGTGGGTCCAACAGAGAAGAAAAAGGTGAGGATTGTTGAAGAATGTGGAGCCACGAGAGCGAAAGCAGTTGAGACGGAGGTTGCTGCTTCGGTTCAGGTTTCGATTATTGAGAGTGATGCTTTGTTGGAGATTGAGTGTTTGCATAGAGAAGGATTGTTGCTTGATGTTATGGTAATGTTGAGGGAGTTGAGGATTGAGGTTATTGGAGTTCAATCTTCTCTTAATAATGGTGTTTTTGTTGCGGAGTTGAGGGCTAAGGTGAAGGAAAATGGTGGAAATGGGAAGAAAGTTAGTATTGTGGAAGTTAAGAAAGCACTTAACCAAATTATACCTCACAACATTTAG
Protein sequence

>MsaT003445.1

MLQAAVQSVQWTYSLFWQLCPQQLILVWGDGYYNGSIKTRKTVQPMEVSAEEASLQRSQQLRELYESLSAGETNPPTRRPCASLSPEDLTESEWFYLMCVSFSFPPGVGLPGRAYTKRQHVWLTGANEVDSKTFSRAILAKSANIQTVVCIPVLDGVVEFGTTDKIQEDLNFIKHVKSFFLDQHSLPPKPALSEHSTSNPTSSTDHIPTIMYTMADPPSTNPNQDDMDEDEEEEEDEEDDEVESESEDEAFKQSELMQIEMPDDIRVGSPNDGSNNLDSDFHLLVVSNQGNPSGHVDSYKTDSTQRWVPIEEPVGNSLQVQLPSSVLHHQLEDLTQEDTHYSQTVSTIIQNQSTQWNIDFSPSINYITYSSQSSFTNWTNHHHFHALPPPETTSTTSQCLLKYILFTVPYLHTKNHDETSPQTHDAGSDPSAKLRGKGTPQDELSANHVLAERRRREKLNERFIILRSLVPFVTKMDKASILGDTIEYLKQLRRKIQDLETRNRQIETEQQSRSGVTVLVGPTEKKKVRIVEECGATRAKAVETEVAASVQVSIIESDALLEIECLHREGLLLDVMVMLRELRIEVIGVQSSLNNGVFVAELRAKVKENGGNGKKVSIVEVKKALNQIIPHNI*