Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003458 | XP_003590676.1 | 93.538 | 325 | 21 | 0 | 1 | 325 | 1 | 325 | 0.0 | 634 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003458 | sp|Q8S3D5|LRL2_ARATH | 36.154 | 130 | 81 | 1 | 190 | 317 | 136 | 265 | 3.37e-17 | 84.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003458 | tr|G7IEF8|G7IEF8_MEDTR | 93.538 | 325 | 21 | 0 | 1 | 325 | 1 | 325 | 0.0 | 634 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG003458 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG003210 | MsaG003458 | 0.810465 | 1.946327e-50 | 3.361735e-48 |
| MsaG003458 | MsaG003459 | 0.820186 | 1.372893e-52 | 3.034540e-50 |
| MsaG003458 | MsaG008551 | 0.823127 | 2.890343e-53 | 6.909513e-51 |
| MsaG003458 | MsaG011806 | 0.800381 | 2.475588e-48 | 3.376040e-46 |
| MsaG003458 | MsaG020983 | 0.815125 | 1.877288e-51 | 3.640315e-49 |
| MsaG003458 | MsaG020984 | 0.815778 | 1.345678e-51 | 2.653020e-49 |
| MsaG003458 | MsaG021863 | 0.802785 | 7.996726e-49 | 1.151746e-46 |
| MsaG003458 | MsaG028034 | 0.830379 | 5.460115e-55 | 1.597479e-52 |
| MsaG003458 | MsaG028716 | 0.829995 | 6.770097e-55 | 1.959085e-52 |
| MsaG003458 | MsaG029320 | 0.808818 | 4.379996e-50 | 7.269572e-48 |
| MsaG003458 | MsaG041899 | 0.809516 | 3.107959e-50 | 5.246102e-48 |
| MsaG003458 | MsaG041901 | 0.800892 | 1.949038e-48 | 2.688783e-46 |
| MsaG003458 | MsaG044439 | 0.811505 | 1.161323e-50 | 2.057524e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003458 | MtrunA17_Chr1g0185741 | 92.739 | 303 | 22 | 0 | 1 | 303 | 1 | 303 | 0.0 | 588 |
| MsaG003458 | MtrunA17_Chr4g0074161 | 33.735 | 166 | 81 | 4 | 181 | 317 | 194 | 359 | 7.38e-18 | 83.6 |
| MsaG003458 | MtrunA17_Chr1g0190731 | 33.577 | 137 | 79 | 1 | 190 | 314 | 199 | 335 | 1.11e-15 | 77.0 |
| MsaG003458 | MtrunA17_Chr1g0184841 | 33.333 | 117 | 66 | 1 | 213 | 317 | 149 | 265 | 1.15e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003458 | AT5G64980.1 | 44.715 | 123 | 66 | 2 | 191 | 311 | 218 | 340 | 1.89e-22 | 96.7 |
| MsaG003458 | AT5G64980.2 | 44.715 | 123 | 66 | 2 | 191 | 311 | 218 | 340 | 1.89e-22 | 96.7 |
| MsaG003458 | AT2G24260.2 | 34.132 | 167 | 83 | 5 | 170 | 317 | 23 | 181 | 3.21e-18 | 82.8 |
| MsaG003458 | AT4G30980.1 | 36.154 | 130 | 81 | 1 | 190 | 317 | 136 | 265 | 3.43e-18 | 84.0 |
| MsaG003458 | AT4G30980.2 | 34.000 | 150 | 94 | 2 | 170 | 317 | 167 | 313 | 6.38e-18 | 84.0 |
| MsaG003458 | AT2G24260.1 | 34.132 | 167 | 83 | 5 | 170 | 317 | 126 | 284 | 1.23e-17 | 82.8 |
| MsaG003458 | AT5G58010.1 | 33.108 | 148 | 75 | 4 | 190 | 315 | 105 | 250 | 2.02e-14 | 73.2 |
| MsaG003458 | AT1G03040.2 | 28.934 | 197 | 120 | 4 | 133 | 311 | 79 | 273 | 3.93e-13 | 69.3 |
| MsaG003458 | AT1G03040.1 | 29.851 | 201 | 116 | 5 | 134 | 311 | 80 | 278 | 4.22e-13 | 69.3 |
| MsaG003458 | AT4G02590.3 | 33.628 | 113 | 61 | 1 | 213 | 311 | 112 | 224 | 1.75e-11 | 63.9 |
| MsaG003458 | AT4G02590.2 | 33.628 | 113 | 61 | 1 | 213 | 311 | 175 | 287 | 1.98e-11 | 64.3 |
| MsaG003458 | AT4G02590.1 | 33.628 | 113 | 61 | 1 | 213 | 311 | 175 | 287 | 1.98e-11 | 64.3 |
Find 53 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGAGCTGCCTCCTGATTT+TGG | 0.146986 | 1:-57145989 | None:intergenic |
| GATCCTGGAAAAGCTGATTT+TGG | 0.197185 | 1:+57145035 | MsaT003458.1:CDS |
| GATTCTTCAAGTCATGATTT+TGG | 0.211671 | 1:+57145071 | MsaT003458.1:CDS |
| TGCTTCACAATTTGATCATA+TGG | 0.268319 | 1:+57145934 | MsaT003458.1:CDS |
| ATGATTTATTCACAGGGTTA+TGG | 0.279023 | 1:+57147108 | MsaT003458.1:intron |
| TTACTTCCAAATCCAAAATC+AGG | 0.321359 | 1:+57145977 | MsaT003458.1:CDS |
| TGAGCCTCTTGAAGAGATAA+TGG | 0.328914 | 1:+57147158 | MsaT003458.1:CDS |
| TGATTTATTCACAGGGTTAT+GGG | 0.337574 | 1:+57147109 | MsaT003458.1:intron |
| TGTTCTGCTGCCTATTGCTT+TGG | 0.355110 | 1:+57147239 | MsaT003458.1:CDS |
| TGCTTATCTCCAACTGAATC+AGG | 0.357388 | 1:-57145908 | None:intergenic |
| TCAGTTGTTGTAGTAGTGTT+TGG | 0.377494 | 1:-57144951 | None:intergenic |
| GAGCCTCTTGAAGAGATAAT+GGG | 0.382191 | 1:+57147159 | MsaT003458.1:CDS |
| TACTACCAAGAGTGTGATAA+TGG | 0.397730 | 1:-57144995 | None:intergenic |
| ACAACTGAGTATAATATGAT+TGG | 0.400574 | 1:+57144966 | MsaT003458.1:CDS |
| TTTGGATTTGGAAGTAAAGA+AGG | 0.414312 | 1:-57145971 | None:intergenic |
| AAACAACAGATTACCACATC+TGG | 0.423786 | 1:-57146279 | None:intergenic |
| GGTTCAACCATTGCATAGCT+TGG | 0.429150 | 1:-57147275 | None:intergenic |
| GCTTCACAATTTGATCATAT+GGG | 0.439933 | 1:+57145935 | MsaT003458.1:CDS |
| GGGTTATGGGCATTATATAA+AGG | 0.442119 | 1:+57147122 | MsaT003458.1:intron |
| AGTTCATCAACCAAAGCAAT+AGG | 0.445410 | 1:-57147249 | None:intergenic |
| GCAATTCATGTAAAGCTTTG+AGG | 0.446403 | 1:-57146248 | None:intergenic |
| TCCCACTTCTTCCAAAGTGT+TGG | 0.449123 | 1:-57144854 | None:intergenic |
| ATTAACTAACCTCAGTATAG+TGG | 0.456570 | 1:-57145291 | None:intergenic |
| GAAGCTCATATATTTGAAAC+CGG | 0.467992 | 1:+57145253 | MsaT003458.1:CDS |
| AAATAGTTAGCTAGCTAGCT+TGG | 0.473472 | 1:-57147296 | None:intergenic |
| CAAATTCAAAACCAACACTT+TGG | 0.479840 | 1:+57144843 | MsaT003458.1:CDS |
| GGGTAGTCAAGCTTACATAT+TGG | 0.487201 | 1:+57146438 | MsaT003458.1:intron |
| AACCAACACTTTGGAAGAAG+TGG | 0.489754 | 1:+57144852 | MsaT003458.1:CDS |
| CAACTGAGTATAATATGATT+GGG | 0.500571 | 1:+57144967 | MsaT003458.1:CDS |
| AGCAGCAGTTTCAATGATCC+TGG | 0.501372 | 1:+57145020 | MsaT003458.1:CDS |
| TTCCCCATTATCTCTTCAAG+AGG | 0.517037 | 1:-57147162 | None:intergenic |
| CAACTTTCATATGAAAATGA+AGG | 0.525784 | 1:+57144789 | MsaT003458.1:CDS |
| AATAGCCATTATCACACTCT+TGG | 0.527667 | 1:+57144990 | MsaT003458.1:CDS |
| GATGTTCCTCAGAAACATCA+TGG | 0.529826 | 1:-57144821 | None:intergenic |
| TTGGAGAAATGATGAAGGAG+AGG | 0.544579 | 1:+57145228 | MsaT003458.1:CDS |
| GTTCCTTTGCAGGAACTAAG+TGG | 0.552010 | 1:+57146642 | MsaT003458.1:intron |
| TTGGAAGAAGTGGGATAGAG+TGG | 0.574092 | 1:+57144862 | MsaT003458.1:CDS |
| ATACTTGCAGATTCAAGTGA+AGG | 0.584258 | 1:+57146483 | MsaT003458.1:CDS |
| CTCTTTGAATACAGCAACGC+AGG | 0.587080 | 1:+57146208 | MsaT003458.1:intron |
| ACATGGACACCTGATTCAGT+TGG | 0.593145 | 1:+57145899 | MsaT003458.1:CDS |
| TGTAATAGGGTCATGCAACA+TGG | 0.593241 | 1:+57145882 | MsaT003458.1:intron |
| GTAGTGAATAAAATAATGGA+AGG | 0.599004 | 1:+57144735 | None:intergenic |
| ACCAACACTTTGGAAGAAGT+GGG | 0.607841 | 1:+57144853 | MsaT003458.1:CDS |
| AGTGTTTGGAAGATAGCATG+AGG | 0.616860 | 1:-57144937 | None:intergenic |
| CTTCCAAATCCAAAATCAGG+AGG | 0.618532 | 1:+57145980 | MsaT003458.1:CDS |
| AAGTAGCCATGATGTTTCTG+AGG | 0.620893 | 1:+57144815 | MsaT003458.1:CDS |
| AGCCTCTTGAAGAGATAATG+GGG | 0.633642 | 1:+57147160 | MsaT003458.1:CDS |
| TGTACCTCATGGAAAACTAG+TGG | 0.648110 | 1:-57146696 | None:intergenic |
| AACTGAACCAAGCTATGCAA+TGG | 0.660513 | 1:+57147268 | MsaT003458.1:CDS |
| CAGATTACCACATCTGGAGT+CGG | 0.663959 | 1:-57146273 | None:intergenic |
| TGTCAGCTACTTGAAAACAA+AGG | 0.685270 | 1:+57147213 | MsaT003458.1:CDS |
| TCAAAATCTCCACTATACTG+AGG | 0.696504 | 1:+57145282 | MsaT003458.1:CDS |
| ATTGCTGCCGACTCCAGATG+TGG | 0.727085 | 1:+57146266 | MsaT003458.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 57144750 | 57147308 | 57144750 | ID=MsaG003458 |
| Chr1 | mRNA | 57144750 | 57147308 | 57144750 | ID=MsaT003458.1;Parent=MsaG003458 |
| Chr1 | exon | 57144750 | 57145103 | 57144750 | ID=MsaT003458.1.exon1;Parent=MsaT003458.1 |
| Chr1 | CDS | 57144750 | 57145103 | 57144750 | ID=cds.MsaT003458.1;Parent=MsaT003458.1 |
| Chr1 | exon | 57145217 | 57145303 | 57145217 | ID=MsaT003458.1.exon2;Parent=MsaT003458.1 |
| Chr1 | CDS | 57145217 | 57145303 | 57145217 | ID=cds.MsaT003458.1;Parent=MsaT003458.1 |
| Chr1 | exon | 57145890 | 57146048 | 57145890 | ID=MsaT003458.1.exon3;Parent=MsaT003458.1 |
| Chr1 | CDS | 57145890 | 57146048 | 57145890 | ID=cds.MsaT003458.1;Parent=MsaT003458.1 |
| Chr1 | exon | 57146222 | 57146287 | 57146222 | ID=MsaT003458.1.exon4;Parent=MsaT003458.1 |
| Chr1 | CDS | 57146222 | 57146287 | 57146222 | ID=cds.MsaT003458.1;Parent=MsaT003458.1 |
| Chr1 | exon | 57146439 | 57146504 | 57146439 | ID=MsaT003458.1.exon5;Parent=MsaT003458.1 |
| Chr1 | CDS | 57146439 | 57146504 | 57146439 | ID=cds.MsaT003458.1;Parent=MsaT003458.1 |
| Chr1 | exon | 57146654 | 57146713 | 57146654 | ID=MsaT003458.1.exon6;Parent=MsaT003458.1 |
| Chr1 | CDS | 57146654 | 57146713 | 57146654 | ID=cds.MsaT003458.1;Parent=MsaT003458.1 |
| Chr1 | exon | 57147123 | 57147308 | 57147123 | ID=MsaT003458.1.exon7;Parent=MsaT003458.1 |
| Chr1 | CDS | 57147123 | 57147308 | 57147123 | ID=cds.MsaT003458.1;Parent=MsaT003458.1 |
| Gene Sequence |
| Protein sequence |
