Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003554 | AES61869.1 | 54.237 | 177 | 30 | 1 | 1 | 177 | 1 | 126 | 4.42e-51 | 171 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003554 | tr|G7I2W4|G7I2W4_MEDTR | 54.237 | 177 | 30 | 1 | 1 | 177 | 1 | 126 | 2.11e-51 | 171 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG003554 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG003554 | MtrunA17_Chr5g0399781 | 53.846 | 52 | 23 | 1 | 4 | 55 | 13 | 63 | 4.28e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTTGTGGTTTACATTTCA+AGG | 0.339580 | 1:+58808552 | MsaT003554.1:CDS |
| ATTGGTGTCGGCGTCTCTAT+TGG | 0.349108 | 1:-58809522 | None:intergenic |
| AATCTTTCAAATCAAACTTC+AGG | 0.363377 | 1:+58808667 | MsaT003554.1:CDS |
| CCACAAGCGTTACACAATAT+TGG | 0.380139 | 1:-58808538 | None:intergenic |
| CCAAGCAATGAGATAGGTCT+TGG | 0.425187 | 1:+58809651 | MsaT003554.1:CDS |
| CACAATATTGGCGTCTCGTA+TGG | 0.440494 | 1:-58808526 | None:intergenic |
| TTGCAACAGAAACTCAAGTT+TGG | 0.443870 | 1:+58808494 | MsaT003554.1:CDS |
| GGTTTACATTTCAAGGCAAA+AGG | 0.472477 | 1:+58808559 | MsaT003554.1:CDS |
| TCAAACAACAAAATATCGTC+AGG | 0.502407 | 1:-58809609 | None:intergenic |
| TCTTGGATGCATTCGTCTAA+AGG | 0.502885 | 1:+58809668 | MsaT003554.1:CDS |
| TTTATACCAAGCAATGAGAT+AGG | 0.511374 | 1:+58809645 | MsaT003554.1:CDS |
| CCAATATTGTGTAACGCTTG+TGG | 0.532300 | 1:+58808538 | MsaT003554.1:CDS |
| GAAACTCAAGTTTGGCGCAA+AGG | 0.544030 | 1:+58808502 | MsaT003554.1:CDS |
| CCAAGACCTATCTCATTGCT+TGG | 0.545705 | 1:-58809651 | None:intergenic |
| AATAGAGACGCCGACACCAA+TGG | 0.575440 | 1:+58809524 | MsaT003554.1:CDS |
| TCTTCGGGAGATAGTTAGCA+AGG | 0.579240 | 1:-58808585 | None:intergenic |
| AAAAGCGTTCATTTGTAGAG+AGG | 0.581892 | 1:+58809475 | MsaT003554.1:CDS |
| GGAAACTCATCCATTGGTGT+CGG | 0.599667 | 1:-58809534 | None:intergenic |
| ATAAGTAGTGTAGAATGGGC+AGG | 0.608004 | 1:+58808905 | MsaT003554.1:intron |
| GATACTTCTACAGATCAAGA+TGG | 0.615323 | 1:+58809696 | MsaT003554.1:CDS |
| CTTCGGGAGATAGTTAGCAA+GGG | 0.642252 | 1:-58808584 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 58808490 | 58809722 | 58808490 | ID=MsaG003554 |
| Chr1 | mRNA | 58808490 | 58809722 | 58808490 | ID=MsaT003554.1;Parent=MsaG003554 |
| Chr1 | exon | 58808490 | 58808688 | 58808490 | ID=MsaT003554.1.exon1;Parent=MsaT003554.1 |
| Chr1 | CDS | 58808490 | 58808688 | 58808490 | ID=cds.MsaT003554.1;Parent=MsaT003554.1 |
| Chr1 | exon | 58808918 | 58808958 | 58808918 | ID=MsaT003554.1.exon2;Parent=MsaT003554.1 |
| Chr1 | CDS | 58808918 | 58808958 | 58808918 | ID=cds.MsaT003554.1;Parent=MsaT003554.1 |
| Chr1 | exon | 58809417 | 58809722 | 58809417 | ID=MsaT003554.1.exon3;Parent=MsaT003554.1 |
| Chr1 | CDS | 58809417 | 58809722 | 58809417 | ID=cds.MsaT003554.1;Parent=MsaT003554.1 |
| Gene Sequence |
| Protein sequence |
