AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003699


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003699 MtrunA17_Chr1g0188651 74.522 157 15 1 1 132 1 157 1.54e-67 201
MsaG003699 MtrunA17_Chr1g0188661 62.264 159 33 2 1 132 1 159 4.96e-56 172
MsaG003699 MtrunA17_Chr4g0012761 62.893 159 32 2 1 132 1 159 5.22e-55 169
MsaG003699 MtrunA17_Chr1g0188681 57.962 157 39 2 1 130 1 157 4.32e-54 167
MsaG003699 MtrunA17_Chr7g0218151 61.006 159 35 2 1 132 1 159 7.48e-53 164
MsaG003699 MtrunA17_Chr1g0193311 64.516 155 28 2 1 128 1 155 2.15e-52 162
MsaG003699 MtrunA17_Chr5g0420351 58.333 156 38 2 1 129 1 156 2.79e-52 162
MsaG003699 MtrunA17_Chr1g0197261 46.795 156 56 2 1 129 1 156 7.84e-39 128
MsaG003699 MtrunA17_Chr1g0197461 47.403 154 54 2 2 128 1 154 1.42e-37 125
MsaG003699 MtrunA17_Chr1g0197321 44.586 157 59 2 1 129 1 157 7.09e-36 120
MsaG003699 MtrunA17_Chr5g0432691 33.974 156 75 2 1 128 1 156 3.38e-27 99.0
MsaG003699 MtrunA17_Chr3g0107381 36.601 153 69 2 1 125 1 153 1.50e-25 96.3
MsaG003699 MtrunA17_Chr4g0014321 31.818 154 76 2 1 125 1 154 2.59e-24 93.2
MsaG003699 MtrunA17_Chr3g0110131 38.854 157 68 3 1 129 1 157 4.45e-22 87.8
MsaG003699 MtrunA17_Chr2g0283941 34.416 154 72 3 1 125 1 154 4.73e-22 87.4
MsaG003699 MtrunA17_Chr4g0013041 32.278 158 70 4 1 126 1 153 3.92e-21 84.3
MsaG003699 MtrunA17_Chr3g0107331 36.076 158 73 2 2 131 1 158 5.27e-21 82.8
MsaG003699 MtrunA17_Chr3g0107461 29.814 161 84 2 1 132 1 161 2.53e-20 81.3
MsaG003699 MtrunA17_Chr8g0351751 57.627 59 25 0 1 59 1 59 6.13e-20 82.0
MsaG003699 MtrunA17_Chr4g0014031 32.919 161 79 2 1 132 1 161 1.56e-18 78.2
MsaG003699 MtrunA17_Chr3g0107351 35.220 159 75 2 2 132 1 159 2.00e-18 76.3
MsaG003699 MtrunA17_Chr3g0107361 33.962 159 77 2 2 132 1 159 4.55e-18 75.5
MsaG003699 MtrunA17_Chr4g0014121 34.375 160 76 2 1 131 29 188 1.35e-17 74.7
MsaG003699 MtrunA17_Chr4g0014131 32.919 161 79 2 1 132 1 161 9.51e-17 73.6
MsaG003699 MtrunA17_Chr3g0107341 45.745 94 48 1 2 92 1 94 3.72e-16 70.5
MsaG003699 MtrunA17_Chr4g0008701 35.433 127 65 3 3 127 6 117 6.81e-16 72.8
MsaG003699 MtrunA17_Chr7g0267601 37.647 85 53 0 3 87 6 90 8.70e-16 72.4
MsaG003699 MtrunA17_Chr2g0296831 35.652 115 48 1 1 89 1 115 2.11e-14 65.1
MsaG003699 MtrunA17_Chr2g0296861 30.323 155 76 3 1 125 1 153 5.64e-14 64.7
MsaG003699 MtrunA17_Chr3g0105791 27.820 133 67 2 29 132 1 133 2.17e-13 62.8
MsaG003699 MtrunA17_Chr4g0031421 27.820 133 67 2 29 132 1 133 1.20e-12 60.8
MsaG003699 MtrunA17_Chr3g0128721 35.294 85 55 0 3 87 6 90 8.69e-12 61.2
MsaG003699 MtrunA17_Chr3g0106501 29.771 131 63 3 29 130 1 131 5.44e-11 56.6
MsaG003699 MtrunA17_Chr3g0106421 30.952 126 58 4 29 125 1 126 7.27e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003699 AT3G05860.2 40.580 138 70 6 1 132 1 132 4.54e-25 95.1
MsaG003699 AT3G05860.3 40.580 138 70 6 1 132 1 132 1.15e-24 95.1
MsaG003699 AT3G05860.1 40.580 138 70 6 1 132 1 132 1.35e-24 95.1
MsaG003699 AT5G48670.1 39.355 155 62 3 1 125 1 153 3.48e-21 87.0
MsaG003699 AT1G22590.2 45.205 73 40 0 1 73 1 73 1.20e-18 77.4
MsaG003699 AT5G27960.1 39.640 111 41 1 4 88 2 112 2.49e-18 79.3
MsaG003699 AT1G65330.1 31.757 148 73 2 2 121 1 148 2.68e-18 78.6
MsaG003699 AT1G65300.1 29.605 152 79 2 2 125 1 152 2.79e-18 78.6
MsaG003699 AT5G26650.1 35.065 154 64 4 4 125 2 151 3.01e-18 79.3
MsaG003699 AT1G31630.1 29.299 157 78 4 2 128 1 154 5.62e-18 78.6
MsaG003699 AT5G26630.1 32.680 153 75 2 1 125 1 153 5.47e-17 74.3
MsaG003699 AT2G28700.1 37.234 94 56 1 1 91 1 94 1.72e-16 74.3
MsaG003699 AT1G31640.1 36.283 113 45 2 2 88 1 112 6.47e-16 73.2
MsaG003699 AT5G26580.1 40.196 102 47 3 1 90 1 100 6.80e-14 67.4

Find 22 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCTAAAGATTCGTATAAT+TGG 0.305787 1:-60802029 MsaT003699.1:CDS
CTCCTTTCTTCTTGAAGCAT+TGG 0.353516 1:+60802325 None:intergenic
GCTTTGGCGTCCTTCAGATT+TGG 0.393835 1:+60802185 None:intergenic
TCTGAAACAGTCAAATCATC+TGG 0.455315 1:+60801990 None:intergenic
CTACCTCTCAATCACCGCTT+TGG 0.465393 1:+60802169 None:intergenic
CTCCAATGCTTCAAGAAGAA+AGG 0.468651 1:-60802327 MsaT003699.1:CDS
CAAGGCCATGGTTCGGAAGA+AGG 0.471800 1:-60802366 None:intergenic
TACATTGCAAGGCCATGGTT+CGG 0.507534 1:-60802373 None:intergenic
GACATACAGAAAGAGAAAGA+AGG 0.512113 1:-60802303 MsaT003699.1:CDS
AGAGAACTCACTATTCTTTG+TGG 0.526508 1:-60802263 MsaT003699.1:CDS
ACATACAGAAAGAGAAAGAA+GGG 0.541098 1:-60802302 MsaT003699.1:CDS
TGCAGCAGATTACTAAAGCT+AGG 0.546331 1:-60802091 MsaT003699.1:intron
AAACAGTCAAATCATCTGGC+AGG 0.548613 1:+60801994 None:intergenic
AGGATCGGAAATTATAGCAC+AGG 0.559456 1:+60802232 None:intergenic
TCGGAAATTATAGCACAGGC+TGG 0.570119 1:+60802236 None:intergenic
AGAGGTGTGGCCAAATCTGA+AGG 0.576828 1:-60802195 MsaT003699.1:CDS
AAAGAAGGGTATCATCAAGA+AGG 0.577075 1:-60802288 MsaT003699.1:CDS
CTTCACCTTCTTCCGAACCA+TGG 0.581108 1:+60802361 None:intergenic
GCAGCAGATTACTAAAGCTA+GGG 0.592338 1:-60802090 MsaT003699.1:intron
GAAACAAGACAATCGTGAGA+AGG 0.628912 1:-60802051 MsaT003699.1:CDS
TTGATTCTAAAACAGAGGTG+TGG 0.633348 1:-60802208 MsaT003699.1:CDS
AAATCTGAAGGACGCCAAAG+CGG 0.705263 1:-60802183 MsaT003699.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 60801908 60802381 60801908 ID=MsaG003699
Chr1 mRNA 60801908 60802381 60801908 ID=MsaT003699.1;Parent=MsaG003699
Chr1 exon 60801908 60802108 60801908 ID=MsaT003699.1.exon2;Parent=MsaT003699.1
Chr1 CDS 60801908 60802108 60801908 ID=cds.MsaT003699.1;Parent=MsaT003699.1
Chr1 exon 60802184 60802381 60802184 ID=MsaT003699.1.exon1;Parent=MsaT003699.1
Chr1 CDS 60802184 60802381 60802184 ID=cds.MsaT003699.1;Parent=MsaT003699.1
Gene Sequence

>MsaT003699.1

ATGGTTCGGAAGAAGGTGAAGCTTGCTTTTATCTCCAATGCTTCAAGAAGAAAGGAGACATACAGAAAGAGAAAGAAGGGTATCATCAAGAAGGTGAGAGAACTCACTATTCTTTGTGGTATTCCAGCCTGTGCTATAATTTCCGATCCTTTTGATTCTAAAACAGAGGTGTGGCCAAATCTGAAGGACGCCAAAGCGCAGATTACTAAAGCTAGGGAACAACTGAAAAAGCTGAAACAAGACAATCGTGAGAAGGAGCTAAAGATTCGTATAATTGGATACATGCAAAACCTGCCAGATGATTTGACTGTTTCAGATTTGAAAGAACTTGATACGATAATTGAGAAAAATATGAAGGAAATTGATGAAAAGATTGTCGCACTCAATTTATCAGATTGA

Protein sequence

>MsaT003699.1

MVRKKVKLAFISNASRRKETYRKRKKGIIKKVRELTILCGIPACAIISDPFDSKTEVWPNLKDAKAQITKAREQLKKLKQDNREKELKIRIIGYMQNLPDDLTVSDLKELDTIIEKNMKEIDEKIVALNLSD*