AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003702


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003702 MtrunA17_Chr1g0188681 90.769 130 12 0 1 130 1 130 3.90e-86 248
MsaG003702 MtrunA17_Chr5g0420351 73.643 129 34 0 1 129 1 129 4.83e-66 197
MsaG003702 MtrunA17_Chr1g0188661 76.154 130 31 0 1 130 1 130 1.06e-63 191
MsaG003702 MtrunA17_Chr7g0218151 74.615 130 33 0 1 130 1 130 3.89e-60 182
MsaG003702 MtrunA17_Chr4g0012761 73.077 130 35 0 1 130 1 130 1.05e-59 181
MsaG003702 MtrunA17_Chr1g0193311 70.769 130 38 0 1 130 1 130 4.89e-56 171
MsaG003702 MtrunA17_Chr1g0188651 70.769 130 36 1 1 130 1 128 5.29e-54 166
MsaG003702 MtrunA17_Chr1g0197321 59.504 121 49 0 1 121 1 121 1.43e-49 155
MsaG003702 MtrunA17_Chr1g0197261 56.923 130 56 0 1 130 1 130 1.85e-48 152
MsaG003702 MtrunA17_Chr1g0197461 57.812 128 54 0 3 130 2 129 8.08e-48 150
MsaG003702 MtrunA17_Chr4g0014321 42.975 121 68 1 1 120 1 121 2.09e-32 114
MsaG003702 MtrunA17_Chr3g0110131 55.372 121 53 1 1 120 1 121 5.95e-32 113
MsaG003702 MtrunA17_Chr4g0014031 51.240 121 58 1 1 120 1 121 4.67e-30 108
MsaG003702 MtrunA17_Chr4g0013041 41.270 126 71 2 1 125 1 124 7.53e-30 107
MsaG003702 MtrunA17_Chr3g0107331 43.750 128 71 1 3 129 2 129 9.95e-30 105
MsaG003702 MtrunA17_Chr3g0107381 45.312 128 69 1 1 127 1 128 1.01e-29 107
MsaG003702 MtrunA17_Chr8g0351751 41.985 131 73 1 1 128 1 131 4.80e-28 103
MsaG003702 MtrunA17_Chr3g0107351 42.188 128 73 1 3 129 2 129 1.52e-27 99.8
MsaG003702 MtrunA17_Chr5g0432691 36.154 130 82 1 1 129 1 130 3.49e-27 99.0
MsaG003702 MtrunA17_Chr2g0296831 44.628 121 66 1 1 120 1 121 5.13e-27 97.1
MsaG003702 MtrunA17_Chr3g0107361 47.273 110 57 1 3 111 2 111 1.08e-26 97.4
MsaG003702 MtrunA17_Chr2g0283941 43.697 119 66 1 1 118 1 119 1.81e-25 96.7
MsaG003702 MtrunA17_Chr3g0107341 39.844 128 76 1 3 129 2 129 3.34e-25 93.6
MsaG003702 MtrunA17_Chr4g0014131 49.587 121 60 1 1 120 1 121 1.05e-23 91.7
MsaG003702 MtrunA17_Chr3g0107461 41.803 122 68 2 1 119 1 122 1.08e-23 89.7
MsaG003702 MtrunA17_Chr4g0014121 44.186 129 71 1 1 128 29 157 2.46e-22 87.0
MsaG003702 MtrunA17_Chr4g0008701 35.000 120 74 2 1 116 1 120 1.08e-17 77.8
MsaG003702 MtrunA17_Chr2g0296861 36.170 94 59 1 1 93 1 94 9.00e-17 72.0
MsaG003702 MtrunA17_Chr7g0267601 32.174 115 77 1 3 116 6 120 1.51e-16 74.7
MsaG003702 MtrunA17_Chr3g0105791 41.414 99 55 2 29 125 1 98 1.10e-15 68.6
MsaG003702 MtrunA17_Chr4g0031421 38.776 98 59 1 29 125 1 98 3.85e-15 67.4
MsaG003702 MtrunA17_Chr3g0106501 35.714 98 62 1 29 125 1 98 1.27e-14 66.2
MsaG003702 MtrunA17_Chr3g0106421 36.275 102 62 2 29 127 1 102 3.08e-13 63.9
MsaG003702 MtrunA17_Chr6g0449861 31.858 113 69 3 1 106 9 120 2.51e-12 62.4
MsaG003702 MtrunA17_Chr3g0128721 29.204 113 79 1 3 114 6 118 2.61e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003702 AT1G65330.1 41.727 139 71 2 3 131 2 140 1.04e-29 108
MsaG003702 AT3G05860.2 43.548 124 69 1 1 123 1 124 3.87e-29 105
MsaG003702 AT3G05860.3 47.273 110 57 1 1 109 1 110 1.30e-28 105
MsaG003702 AT3G05860.1 47.273 110 57 1 1 109 1 110 2.03e-28 104
MsaG003702 AT5G48670.1 53.097 113 52 1 1 112 1 113 1.65e-27 103
MsaG003702 AT1G65300.1 41.441 111 64 1 3 112 2 112 2.16e-27 102
MsaG003702 AT5G26630.1 45.238 126 68 1 1 125 1 126 6.85e-26 97.4
MsaG003702 AT5G26650.1 45.794 107 57 1 4 109 2 108 6.95e-26 100
MsaG003702 AT5G27960.1 43.925 107 59 1 4 109 2 108 7.87e-25 96.7
MsaG003702 AT1G31630.1 36.937 111 68 2 3 112 2 111 1.32e-21 88.2
MsaG003702 AT1G31640.1 40.870 115 66 2 3 116 2 115 2.13e-20 85.9
MsaG003702 AT1G22590.2 33.043 115 76 1 1 114 1 115 1.23e-19 79.7
MsaG003702 AT5G27810.1 44.086 93 51 1 29 120 1 93 2.13e-19 78.2
MsaG003702 AT2G28700.1 37.190 121 75 1 1 120 1 121 5.32e-19 81.3
MsaG003702 AT5G26580.1 39.450 109 53 1 1 109 1 96 3.61e-18 79.0
MsaG003702 AT2G40210.1 36.923 130 77 2 1 127 1 128 7.29e-16 72.8
MsaG003702 AT5G06500.1 34.109 129 82 2 1 127 1 128 8.87e-15 68.6
MsaG003702 AT5G58890.1 31.532 111 68 3 1 104 1 110 5.22e-11 58.9

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGCAAAAGAAAACTTTCT+TGG 0.221526 1:+60846794 None:intergenic
CCAAGAGTTGCCTGATGATT+TGG 0.265725 1:-60846680 MsaT003702.1:CDS
AGCCTTTCTTGCCGAATCAT+TGG 0.368149 1:+60847011 None:intergenic
TGTATGGCTCCCTCTAGATT+TGG 0.415378 1:+60846871 None:intergenic
CTCCAATGATTCGGCAAGAA+AGG 0.422860 1:-60847013 MsaT003702.1:CDS
CCAAATCATCAGGCAACTCT+TGG 0.424898 1:+60846680 None:intergenic
AATCAGAATACCAAATCATC+AGG 0.453928 1:+60846670 None:intergenic
AGAGAGCTCTCTATTCTTTG+TGG 0.458868 1:-60846949 MsaT003702.1:CDS
AAATCTAGAGGGAGCCATAC+AGG 0.466931 1:-60846869 MsaT003702.1:CDS
TTTCACCTTCTTCCTAGTCA+TGG 0.474514 1:+60847047 None:intergenic
AAAGAAGGGTATCATTAAGA+AGG 0.475254 1:-60846974 MsaT003702.1:CDS
TGTTATAGAATCAAACGGAT+TGG 0.483520 1:+60846903 None:intergenic
ATACCTCTCAATCACCTGTA+TGG 0.492173 1:+60846855 None:intergenic
GCAGCAGATTACTAAAACCA+GGG 0.508508 1:-60846776 MsaT003702.1:CDS
GAATGAGCTGGACATTCATA+TGG 0.511354 1:-60846719 MsaT003702.1:CDS
TGCAGCAGATTACTAAAACC+AGG 0.515480 1:-60846777 MsaT003702.1:CDS
TAGCTACAACAAGAGAAAGA+AGG 0.531524 1:-60846989 MsaT003702.1:CDS
ACCTCTGTTATAGAATCAAA+CGG 0.538136 1:+60846898 None:intergenic
CGGATTGGAAATTATAGCAC+AGG 0.556765 1:+60846918 None:intergenic
AGCTACAACAAGAGAAAGAA+GGG 0.560386 1:-60846988 MsaT003702.1:CDS
TCCGTTTGATTCTATAACAG+AGG 0.561391 1:-60846899 MsaT003702.1:CDS
ACAGAATAATGAGAATGAGC+TGG 0.567812 1:-60846731 MsaT003702.1:CDS
TTGGAAATTATAGCACAGGC+TGG 0.567823 1:+60846922 None:intergenic
AGAGGTGTGGCCAAATCTAG+AGG 0.598360 1:-60846881 MsaT003702.1:CDS
AGCTGGACATTCATATGGTT+AGG 0.635359 1:-60846714 MsaT003702.1:CDS
GAGCCATACAGGTGATTGAG+AGG 0.637552 1:-60846858 MsaT003702.1:CDS
GAGGTGTGGCCAAATCTAGA+GGG 0.655326 1:-60846880 MsaT003702.1:CDS
TTGATTCTATAACAGAGGTG+TGG 0.661112 1:-60846894 MsaT003702.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 60846672 60847067 60846672 ID=MsaG003702
Chr1 mRNA 60846672 60847067 60846672 ID=MsaT003702.1;Parent=MsaG003702
Chr1 exon 60846672 60847067 60846672 ID=MsaT003702.1.exon1;Parent=MsaT003702.1
Chr1 CDS 60846672 60847067 60846672 ID=cds.MsaT003702.1;Parent=MsaT003702.1
Gene Sequence

>MsaT003702.1

ATGACTAGGAAGAAGGTGAAACTTGCTTTTATCTCCAATGATTCGGCAAGAAAGGCTAGCTACAACAAGAGAAAGAAGGGTATCATTAAGAAGGTGAGAGAGCTCTCTATTCTTTGTGGTATTCCAGCCTGTGCTATAATTTCCAATCCGTTTGATTCTATAACAGAGGTGTGGCCAAATCTAGAGGGAGCCATACAGGTGATTGAGAGGTATCAAAATGCATATATCAAACACAAGAAAAATAATGTGAACCAAGAAAGTTTTCTTTTGCAGCAGATTACTAAAACCAGGGAACAACTAGAAAAGCAAAGACAACAGAATAATGAGAATGAGCTGGACATTCATATGGTTAGGCACTTGCAAAACCAAGAGTTGCCTGATGATTTGGTATTCTGA

Protein sequence

>MsaT003702.1

MTRKKVKLAFISNDSARKASYNKRKKGIIKKVRELSILCGIPACAIISNPFDSITEVWPNLEGAIQVIERYQNAYIKHKKNNVNQESFLLQQITKTREQLEKQRQQNNENELDIHMVRHLQNQELPDDLVF*