AlfalfaGEDB | MsaG003717

M. sativa ssp. caerulea / MsaG003717

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003717	XP_013468831.1	67.857	112	11	1	1	87	1	112	9.80e-43	147

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003717	sp\|O80805\|PHE1_ARATH	52.113	71	34	0	3	73	2	72	1.60e-19	83.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003717	tr\|A0A072VNB0\|A0A072VNB0_MEDTR	67.857	112	11	1	1	87	1	112	4.68e-43	147

TFs/TRs:

Gene ID	Type	Classification
MsaG003717	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000115	MsaG003717	0.806171	1.585946e-49	2.471211e-47
MsaG000535	MsaG003717	0.819096	2.428598e-52	5.215697e-50
MsaG000689	MsaG003717	0.811261	1.311340e-50	2.309440e-48
MsaG003329	MsaG003717	0.810202	2.215974e-50	3.802896e-48
MsaG003717	MsaG004441	0.808356	5.490384e-50	9.011429e-48
MsaG003717	MsaG005495	0.817763	4.851037e-52	1.006346e-49
MsaG003717	MsaG005593	0.820454	1.192772e-52	2.655087e-50
MsaG003717	MsaG008126	0.805019	2.760179e-49	4.186569e-47
MsaG003717	MsaG009725	0.816613	8.776714e-52	1.767551e-49
MsaG003717	MsaG009792	0.803435	5.876929e-49	8.592320e-47
MsaG003717	MsaG011861	0.854159	2.855661e-61	1.790378e-58
MsaG003717	MsaG011873	0.824880	1.126123e-53	2.823472e-51
MsaG003717	MsaG012308	0.817055	6.989383e-52	1.423809e-49
MsaG003717	MsaG013530	0.809909	2.560800e-50	4.363596e-48
MsaG003717	MsaG014318	0.812679	6.459071e-51	1.178002e-48
MsaG003717	MsaG016397	0.806557	1.316300e-49	2.069879e-47
MsaG003717	MsaG018107	0.802050	1.131433e-48	1.602396e-46
MsaG003717	MsaG018595	0.803044	7.074319e-49	1.025002e-46
MsaG003717	MsaG020215	0.823498	2.369859e-53	5.722441e-51
MsaG003717	MsaG020596	0.843390	2.691658e-58	1.170342e-55
MsaG003717	MsaG026812	0.809612	2.965422e-50	5.016846e-48
MsaG003717	MsaG027376	0.802201	1.054048e-48	1.497927e-46
MsaG003717	MsaG027777	0.813346	4.619917e-51	8.567127e-49
MsaG003717	MsaG029391	0.820402	1.225249e-52	2.723657e-50
MsaG003717	MsaG033700	0.817031	7.077276e-52	1.440825e-49
MsaG003717	MsaG034047	0.811277	1.300557e-50	2.291360e-48
MsaG003717	MsaG044190	0.807156	9.847024e-50	1.570417e-47
MsaG003717	MsaG046266	0.820708	1.043418e-52	2.338077e-50
MsaG003717	MsaG002309	0.812159	8.380596e-51	1.508764e-48
MsaG003717	MsaG003279	0.836497	1.653380e-56	5.792779e-54
MsaG003717	MsaG003540	0.887665	2.544522e-72	6.653627e-69
MsaG003717	MsaG012729	0.803196	6.582446e-49	9.571080e-47
MsaG003717	MsaG012523	0.822264	4.579753e-53	1.069588e-50
MsaG003717	MsaG018513	0.802445	9.390936e-49	1.342122e-46
MsaG003717	MsaG029566	0.837841	7.521679e-57	2.745130e-54
MsaG003717	MsaG027639	0.813102	5.224350e-51	9.629095e-49
MsaG003717	MsaG026806	0.803516	5.656495e-49	8.285502e-47
MsaG003717	MsaG031891	0.803132	6.784858e-49	9.850726e-47
MsaG003717	MsaG036754	0.809438	3.230102e-50	5.441881e-48
MsaG003717	MsaG038027	0.842357	5.053578e-58	2.125714e-55
MsaG003717	MsaG039898	0.818984	2.573440e-52	5.510605e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003717	MtrunA17_Chr1g0188681	67.857	112	11	1	1	87	1	112	9.01e-47	147
MsaG003717	MtrunA17_Chr5g0420351	61.207	116	20	1	1	91	1	116	3.06e-42	135
MsaG003717	MtrunA17_Chr1g0188661	61.864	118	19	2	1	92	1	118	1.68e-35	118
MsaG003717	MtrunA17_Chr1g0197321	50.847	118	32	2	1	93	1	117	1.40e-33	113
MsaG003717	MtrunA17_Chr4g0012761	59.322	118	22	2	1	92	1	118	1.82e-33	113
MsaG003717	MtrunA17_Chr7g0218151	58.120	117	24	1	1	92	1	117	6.10e-33	112
MsaG003717	MtrunA17_Chr1g0193311	56.452	124	28	2	1	99	1	123	1.84e-32	110
MsaG003717	MtrunA17_Chr1g0197261	50.893	112	30	1	1	87	1	112	1.35e-30	106
MsaG003717	MtrunA17_Chr1g0188651	60.360	111	19	1	1	86	1	111	2.60e-30	105
MsaG003717	MtrunA17_Chr1g0197461	65.000	80	28	0	3	82	2	81	4.92e-30	104
MsaG003717	MtrunA17_Chr4g0013041	38.400	125	49	2	1	99	1	123	1.78e-22	86.7
MsaG003717	MtrunA17_Chr4g0014321	48.101	79	41	0	1	79	1	79	2.80e-22	86.7
MsaG003717	MtrunA17_Chr8g0351751	55.714	70	31	0	1	70	1	70	3.74e-22	86.7
MsaG003717	MtrunA17_Chr3g0110131	47.458	118	36	1	1	92	1	118	6.51e-21	83.2
MsaG003717	MtrunA17_Chr3g0107331	63.793	58	21	0	3	60	2	59	2.80e-20	79.7
MsaG003717	MtrunA17_Chr4g0014031	45.133	113	36	1	1	87	1	113	4.61e-20	80.9
MsaG003717	MtrunA17_Chr3g0107381	40.678	118	44	2	1	92	1	118	7.57e-20	80.1
MsaG003717	MtrunA17_Chr2g0283941	41.880	117	42	2	1	91	1	117	2.83e-19	79.0
MsaG003717	MtrunA17_Chr3g0107351	62.069	58	22	0	3	60	2	59	4.62e-19	76.6
MsaG003717	MtrunA17_Chr3g0107361	63.158	57	21	0	3	59	2	58	6.66e-19	76.3
MsaG003717	MtrunA17_Chr5g0432691	48.438	64	33	0	1	64	1	64	2.09e-18	75.1
MsaG003717	MtrunA17_Chr2g0296831	50.000	86	41	1	1	84	1	86	7.85e-18	72.4
MsaG003717	MtrunA17_Chr3g0107341	60.345	58	23	0	3	60	2	59	1.18e-17	73.2
MsaG003717	MtrunA17_Chr4g0008701	40.426	94	52	2	1	91	1	93	3.09e-15	69.7
MsaG003717	MtrunA17_Chr3g0107461	45.833	72	39	0	1	72	1	72	3.89e-15	66.6
MsaG003717	MtrunA17_Chr7g0267601	38.298	94	54	2	1	91	1	93	1.42e-14	67.8
MsaG003717	MtrunA17_Chr4g0014121	43.363	113	38	1	1	87	29	141	4.39e-14	64.3
MsaG003717	MtrunA17_Chr4g0014131	42.735	117	41	1	1	91	1	117	4.62e-14	65.1
MsaG003717	MtrunA17_Chr2g0296861	42.188	64	37	0	1	64	1	64	1.46e-13	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG003717	AT3G05860.2	51.852	81	38	1	1	81	1	80	2.94e-25	94.4
MsaG003717	AT3G05860.3	51.852	81	38	1	1	81	1	80	5.03e-25	94.7
MsaG003717	AT3G05860.1	51.852	81	38	1	1	81	1	80	8.14e-25	94.4
MsaG003717	AT1G65330.1	52.113	71	34	0	3	73	2	72	1.63e-20	83.2
MsaG003717	AT5G26650.1	38.261	115	45	1	4	92	2	116	4.37e-20	83.2
MsaG003717	AT5G27960.1	38.261	115	45	1	4	92	2	116	7.61e-20	82.0
MsaG003717	AT1G65300.1	35.345	116	49	1	3	92	2	117	1.83e-19	80.5
MsaG003717	AT5G48670.1	46.903	113	34	1	1	87	1	113	1.84e-18	78.2
MsaG003717	AT1G31630.1	34.483	116	49	3	3	92	2	116	6.49e-18	77.0
MsaG003717	AT1G22590.2	47.541	61	32	0	1	61	1	61	4.09e-17	72.0
MsaG003717	AT2G28700.1	40.698	86	49	2	1	85	1	85	3.72e-16	72.0
MsaG003717	AT5G26580.1	37.500	104	53	1	1	92	1	104	1.36e-15	70.9
MsaG003717	AT1G31640.1	34.483	116	49	2	3	92	2	116	1.63e-15	70.9
MsaG003717	AT5G26630.1	45.349	86	45	1	1	84	1	86	3.34e-15	68.2
MsaG003717	AT2G40210.1	38.636	88	51	1	1	85	1	88	3.83e-13	63.9
MsaG003717	AT5G06500.1	45.312	64	35	0	1	64	1	64	3.09e-11	58.2

Find 24 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAATAATGAGAATGAAATT+TGG	0.296551	1:+61039690	MsaT003717.1:CDS
ATGAAATTTGGACATTCATA+TGG	0.355921	1:+61039702	MsaT003717.1:CDS
AGCCTTTCTTGCCGAATCAT+TGG	0.368149	1:-61039408	None:intergenic
TGTATGGCTCCCTCTAGATT+TGG	0.415378	1:-61039548	None:intergenic
CTCCAATGATTCGGCAAGAA+AGG	0.422860	1:+61039406	MsaT003717.1:CDS
AGAGAGCTCTCTATTCTTTG+TGG	0.458868	1:+61039470	MsaT003717.1:CDS
AAATCTAGAGGGAGCCATAC+AGG	0.466931	1:+61039550	MsaT003717.1:CDS
TTTCACCTTCTTCCTAGTCA+TGG	0.474514	1:-61039372	None:intergenic
AAAGAAGGGTATCATTAAGA+AGG	0.475254	1:+61039445	MsaT003717.1:CDS
ACCAAATCATCAGTAACTCT+TGG	0.476287	1:-61039741	None:intergenic
TGTTATAGAATCAAACGGAT+TGG	0.483520	1:-61039516	None:intergenic
ATACCTCTCAATCACCTGTA+TGG	0.492173	1:-61039564	None:intergenic
GCAGCAGATTACTAAAACCA+GGG	0.508508	1:+61039643	MsaT003717.1:intron
TGCAGCAGATTACTAAAACC+AGG	0.515480	1:+61039642	MsaT003717.1:intron
ATTTGGACATTCATATGGTT+AGG	0.523333	1:+61039707	MsaT003717.1:CDS
TAGCTACAACAAGAGAAAGA+AGG	0.531524	1:+61039430	MsaT003717.1:CDS
ACCTCTGTTATAGAATCAAA+CGG	0.538136	1:-61039521	None:intergenic
CGGATTGGAAATTATAGCAC+AGG	0.556765	1:-61039501	None:intergenic
AGCTACAACAAGAGAAAGAA+GGG	0.560386	1:+61039431	MsaT003717.1:CDS
TCCGTTTGATTCTATAACAG+AGG	0.561391	1:+61039520	MsaT003717.1:CDS
TTGGAAATTATAGCACAGGC+TGG	0.567823	1:-61039497	None:intergenic
AGAGGTGTGGCCAAATCTAG+AGG	0.598360	1:+61039538	MsaT003717.1:CDS
GAGGTGTGGCCAAATCTAGA+GGG	0.655326	1:+61039539	MsaT003717.1:CDS
TTGATTCTATAACAGAGGTG+TGG	0.661112	1:+61039525	MsaT003717.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	61039374	61039754	61039374	ID=MsaG003717
Chr1	mRNA	61039374	61039754	61039374	ID=MsaT003717.1;Parent=MsaG003717
Chr1	exon	61039374	61039571	61039374	ID=MsaT003717.1.exon1;Parent=MsaT003717.1
Chr1	CDS	61039374	61039571	61039374	ID=cds.MsaT003717.1;Parent=MsaT003717.1
Chr1	exon	61039647	61039754	61039647	ID=MsaT003717.1.exon2;Parent=MsaT003717.1
Chr1	CDS	61039647	61039754	61039647	ID=cds.MsaT003717.1;Parent=MsaT003717.1

Gene Sequence	>MsaT003717.1 ATGACTAGGAAGAAGGTGAAACTTGCTTTTATCTCCAATGATTCGGCAAGAAAGGCTAGCTACAACAAGAGAAAGAAGGGTATCATTAAGAAGGTGAGAGAGCTCTCTATTCTTTGTGGTATTCCAGCCTGTGCTATAATTTCCAATCCGTTTGATTCTATAACAGAGGTGTGGCCAAATCTAGAGGGAGCCATACAGCAGATTACTAAAACCAGGGAACAACTAGAAAAGCAAAGACAACAGAATAATGAGAATGAAATTTGGACATTCATATGGTTAGGCACTTGCAAAACCAAGAGTTACTGA
Protein sequence	>MsaT003717.1 MTRKKVKLAFISNDSARKASYNKRKKGIIKKVRELSILCGIPACAIISNPFDSITEVWPNLEGAIQQITKTREQLEKQRQQNNENEIWTFIWLGTCKTKSY*