AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG003830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003830 MtrunA17_Chr1g0190301 97.938 388 8 0 1 388 1 388 0.0 776
MsaG003830 MtrunA17_Chr7g0274471 54.415 419 134 11 8 385 37 439 8.52e-129 377
MsaG003830 MtrunA17_Chr7g0261911 44.656 421 177 11 21 388 72 489 7.48e-95 292
MsaG003830 MtrunA17_Chr7g0258551 58.955 134 54 1 174 307 211 343 3.57e-51 176
MsaG003830 MtrunA17_Chr1g0175091 55.488 164 59 5 166 329 253 402 3.94e-50 174
MsaG003830 MtrunA17_Chr2g0291021 55.921 152 63 2 175 322 49 200 5.29e-47 163
MsaG003830 MtrunA17_Chr7g0254981 52.903 155 70 2 174 325 49 203 1.14e-44 155
MsaG003830 MtrunA17_Chr6g0461641 45.026 191 64 5 171 327 38 221 1.36e-40 148
MsaG003830 MtrunA17_Chr2g0321461 43.558 163 72 2 161 303 31 193 1.32e-36 137
MsaG003830 MtrunA17_Chr5g0409731 42.857 182 81 5 168 326 18 199 1.92e-34 129
MsaG003830 MtrunA17_Chr5g0417521 40.625 160 77 3 171 314 11 168 4.24e-34 129
MsaG003830 MtrunA17_Chr8g0356501 42.759 145 74 2 165 303 27 168 6.45e-34 129
MsaG003830 MtrunA17_Chr1g0175071 46.479 142 75 1 165 306 167 307 8.34e-34 128
MsaG003830 MtrunA17_Chr4g0042451 41.176 170 78 4 171 325 13 175 1.12e-33 128
MsaG003830 MtrunA17_Chr2g0310011 45.985 137 71 2 165 298 25 161 2.13e-32 124
MsaG003830 MtrunA17_Chr7g0242521 40.373 161 91 3 175 331 13 172 1.43e-30 117
MsaG003830 MtrunA17_Chr2g0290991 41.781 146 73 3 167 300 31 176 1.62e-28 113
MsaG003830 MtrunA17_Chr1g0197171 40.559 143 76 2 167 300 36 178 2.79e-28 112
MsaG003830 MtrunA17_Chr1g0175081 55.263 76 33 1 164 239 12 86 8.30e-24 94.7
MsaG003830 MtrunA17_Chr7g0258531 42.636 129 71 2 174 300 212 339 5.21e-23 99.0
MsaG003830 MtrunA17_Chr1g0162931 69.643 56 17 0 176 231 19 74 3.05e-20 89.7
MsaG003830 MtrunA17_Chr1g0210911 67.857 56 18 0 176 231 23 78 6.28e-20 89.7
MsaG003830 MtrunA17_Chr3g0095611 59.649 57 23 0 175 231 19 75 1.21e-19 86.7
MsaG003830 MtrunA17_Chr2g0288601 63.793 58 21 0 176 233 72 129 2.45e-19 83.6
MsaG003830 MtrunA17_Chr7g0243081 59.701 67 26 1 166 231 61 127 6.35e-19 87.0
MsaG003830 MtrunA17_Chr2g0320231 61.194 67 25 1 166 231 87 153 1.14e-18 86.7
MsaG003830 MtrunA17_Chr8g0379121 64.286 56 20 0 176 231 112 167 2.26e-18 86.3
MsaG003830 MtrunA17_Chr3g0109981 42.982 114 49 3 143 254 254 353 2.44e-18 86.3
MsaG003830 MtrunA17_Chr1g0154261 60.938 64 25 0 176 239 127 190 3.90e-18 84.0
MsaG003830 MtrunA17_Chr8g0366281 57.534 73 29 1 169 239 167 239 8.60e-18 84.0
MsaG003830 MtrunA17_Chr6g0479431 62.500 64 22 1 176 237 203 266 1.23e-17 83.6
MsaG003830 MtrunA17_Chr5g0410741 63.333 60 22 0 172 231 216 275 3.78e-17 82.4
MsaG003830 MtrunA17_Chr2g0283951 58.571 70 28 1 157 225 70 139 5.39e-17 81.6
MsaG003830 MtrunA17_Chr4g0062921 48.235 85 42 1 166 248 226 310 2.75e-15 77.0
MsaG003830 MtrunA17_Chr7g0275401 60.345 58 22 1 171 228 188 244 1.35e-14 73.9
MsaG003830 MtrunA17_Chr2g0304841 58.621 58 23 1 171 228 209 265 1.42e-14 75.5
MsaG003830 MtrunA17_Chr4g0009901 50.000 76 36 2 171 246 164 237 2.60e-14 72.8
MsaG003830 MtrunA17_Chr4g0045361 61.111 54 21 0 175 228 274 327 1.53e-13 71.6
MsaG003830 MtrunA17_Chr1g0149841 52.941 68 31 1 171 238 187 253 4.73e-13 70.5
MsaG003830 MtrunA17_Chr3g0137101 56.897 58 24 1 171 228 194 250 4.93e-13 70.5
MsaG003830 MtrunA17_Chr5g0422621 58.929 56 23 0 173 228 196 251 6.15e-13 69.3
MsaG003830 MtrunA17_Chr1g0198601 49.231 65 33 0 173 237 209 273 8.33e-13 69.3
MsaG003830 MtrunA17_Chr5g0402631 53.731 67 30 1 172 237 211 277 8.96e-13 68.9
MsaG003830 MtrunA17_Chr4g0067981 43.478 92 43 2 175 258 215 305 1.47e-12 69.3
MsaG003830 MtrunA17_Chr4g0031961 50.000 72 34 2 158 228 113 183 1.61e-12 67.8
MsaG003830 MtrunA17_Chr4g0074401 50.000 64 31 1 171 234 199 261 2.59e-12 68.2
MsaG003830 MtrunA17_Chr1g0149831 55.932 59 22 1 170 228 123 177 2.90e-12 65.9
MsaG003830 MtrunA17_Chr4g0054981 55.172 58 25 1 171 228 306 362 4.10e-12 67.8
MsaG003830 MtrunA17_Chr1g0161431 52.941 68 25 2 161 228 192 252 4.16e-12 67.8
MsaG003830 MtrunA17_Chr4g0074411 51.562 64 30 1 171 234 197 259 5.02e-12 67.4
MsaG003830 MtrunA17_Chr4g0009981 53.448 58 26 1 171 228 112 168 6.22e-12 65.5
MsaG003830 MtrunA17_Chr7g0255711 55.556 54 23 1 175 228 91 143 1.03e-11 65.1
MsaG003830 MtrunA17_Chr4g0074421 53.571 56 25 1 170 225 128 182 1.20e-11 63.5
MsaG003830 MtrunA17_Chr2g0294911 50.847 59 28 1 170 228 206 263 3.52e-11 64.7
MsaG003830 MtrunA17_Chr4g0040481 49.153 59 29 1 173 231 156 213 4.04e-11 64.3
MsaG003830 MtrunA17_Chr2g0324891 49.153 59 29 1 173 231 159 216 4.66e-11 63.9
MsaG003830 MtrunA17_Chr5g0400241 53.448 58 26 1 171 228 307 363 6.33e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG003830 AT5G29000.1 56.972 251 99 5 61 306 76 322 8.49e-84 260
MsaG003830 AT5G29000.3 56.972 251 99 5 61 306 76 322 8.49e-84 260
MsaG003830 AT5G29000.2 56.972 251 99 5 61 306 119 365 2.20e-83 260
MsaG003830 AT4G28610.1 49.020 306 131 9 17 304 57 355 3.34e-77 244
MsaG003830 AT3G04450.4 50.365 274 120 7 43 309 71 335 2.23e-76 242
MsaG003830 AT3G04450.2 50.365 274 120 7 43 309 71 335 2.23e-76 242
MsaG003830 AT3G04450.1 50.545 275 118 7 43 309 111 375 4.84e-76 242
MsaG003830 AT2G20400.1 49.333 225 95 4 93 304 142 360 1.73e-56 190
MsaG003830 AT2G20400.2 49.115 226 95 5 93 304 142 361 1.04e-54 186
MsaG003830 AT2G01060.1 58.125 160 58 3 161 315 5 160 2.21e-51 174
MsaG003830 AT5G29000.4 52.778 180 79 3 61 238 119 294 1.15e-49 170
MsaG003830 AT5G06800.1 57.343 143 60 1 167 309 184 325 3.66e-49 170
MsaG003830 AT5G06800.2 55.405 148 60 2 167 309 184 330 7.26e-47 164
MsaG003830 AT3G04450.3 47.000 200 93 6 43 238 111 301 1.16e-46 162
MsaG003830 AT3G13040.3 56.643 143 57 1 170 307 236 378 6.70e-43 155
MsaG003830 AT3G13040.1 56.643 143 57 1 170 307 236 378 6.70e-43 155
MsaG003830 AT3G13040.2 56.643 143 57 1 170 307 236 378 6.70e-43 155
MsaG003830 AT4G13640.1 42.105 209 96 6 161 356 22 218 6.45e-42 149
MsaG003830 AT3G24120.1 45.882 170 78 4 162 317 27 196 3.88e-40 144
MsaG003830 AT4G13640.2 41.509 212 96 7 161 356 22 221 8.25e-40 144
MsaG003830 AT3G24120.2 45.087 173 78 5 162 317 27 199 4.82e-38 139
MsaG003830 AT3G04030.3 44.304 158 65 2 165 303 35 188 7.65e-38 140
MsaG003830 AT5G18240.1 45.122 164 63 3 161 303 31 188 2.73e-37 139
MsaG003830 AT5G18240.4 45.122 164 63 3 161 303 31 188 2.73e-37 139
MsaG003830 AT1G69580.1 44.311 167 73 2 167 316 26 189 3.58e-37 137
MsaG003830 AT1G69580.2 44.706 170 69 5 167 316 26 190 1.24e-36 136
MsaG003830 AT3G04030.2 43.038 158 66 3 165 303 35 187 8.97e-35 132
MsaG003830 AT1G79430.2 44.295 149 74 3 165 307 24 169 2.70e-34 130
MsaG003830 AT5G18240.5 44.512 164 62 4 161 303 31 186 3.03e-34 131
MsaG003830 AT5G45580.2 40.373 161 78 1 165 307 13 173 3.74e-33 125
MsaG003830 AT3G04030.1 41.772 158 63 3 165 303 35 182 3.81e-32 125
MsaG003830 AT5G45580.1 40.645 155 74 1 171 307 3 157 5.32e-32 122
MsaG003830 AT5G18240.2 42.683 164 61 4 161 303 31 182 1.23e-31 124
MsaG003830 AT5G18240.3 42.683 164 61 4 161 303 31 182 1.23e-31 124
MsaG003830 AT2G01060.2 56.757 111 43 2 210 315 1 111 8.16e-31 118
MsaG003830 AT3G12730.1 39.752 161 89 4 159 311 7 167 5.12e-30 115
MsaG003830 AT5G06800.3 61.644 73 28 0 167 239 184 256 3.17e-25 103
MsaG003830 AT1G14600.1 69.643 56 17 0 176 231 24 79 9.11e-20 88.2
MsaG003830 AT2G42660.1 56.923 65 28 0 176 240 51 115 1.15e-19 88.2
MsaG003830 AT4G04605.1 49.425 87 43 1 163 248 1 87 4.24e-19 85.5
MsaG003830 AT5G42630.2 60.938 64 25 0 176 239 106 169 2.22e-18 83.6
MsaG003830 AT2G02060.2 67.925 53 17 0 176 228 30 82 3.17e-18 84.0
MsaG003830 AT2G02060.1 67.925 53 17 0 176 228 30 82 3.17e-18 84.0
MsaG003830 AT2G40260.1 60.606 66 25 1 167 231 73 138 3.68e-18 85.9
MsaG003830 AT5G42630.1 60.938 64 25 0 176 239 106 169 5.17e-18 84.0
MsaG003830 AT4G17695.1 62.121 66 23 1 176 239 165 230 5.53e-18 84.3
MsaG003830 AT3G04030.5 39.655 116 47 2 207 303 1 112 2.15e-17 82.8
MsaG003830 AT5G16560.1 57.143 77 30 2 172 245 216 292 3.15e-17 83.2
MsaG003830 AT1G32240.2 63.333 60 22 0 172 231 210 269 3.43e-17 82.8
MsaG003830 AT2G06020.1 52.308 65 31 0 167 231 78 142 4.02e-17 81.6
MsaG003830 AT1G32240.1 63.333 60 22 0 172 231 210 269 4.11e-17 82.8
MsaG003830 AT2G38300.1 60.714 56 22 0 176 231 55 110 4.42e-17 82.0
MsaG003830 AT1G79430.1 38.318 107 57 3 207 307 1 104 9.75e-16 77.4
MsaG003830 AT4G04580.1 55.385 65 28 1 176 240 15 78 1.62e-15 74.3
MsaG003830 AT2G03500.1 57.746 71 28 1 169 237 225 295 1.92e-15 77.8
MsaG003830 AT1G25550.1 57.143 63 27 0 172 234 206 268 7.25e-15 75.5
MsaG003830 AT4G37180.1 52.308 65 31 0 164 228 199 263 7.46e-14 72.8
MsaG003830 AT4G37180.2 52.308 65 31 0 164 228 206 270 8.54e-14 72.4
MsaG003830 AT1G68670.1 51.515 66 32 0 172 237 212 277 1.50e-13 71.6
MsaG003830 AT4G31920.1 48.101 79 39 2 171 249 179 255 1.91e-13 72.0
MsaG003830 AT3G16857.3 64.815 54 18 1 175 228 167 219 5.81e-13 70.9
MsaG003830 AT3G16857.2 64.815 54 18 1 175 228 237 289 6.24e-13 70.9
MsaG003830 AT3G16857.1 64.815 54 18 1 175 228 237 289 6.71e-13 70.9
MsaG003830 AT5G58080.1 57.895 57 23 1 172 228 191 246 8.82e-13 70.1
MsaG003830 AT4G04555.1 47.143 70 36 1 169 238 8 76 9.39e-13 64.7
MsaG003830 AT5G44190.1 54.237 59 26 1 173 231 145 202 1.51e-12 68.9
MsaG003830 AT2G25180.1 59.322 59 23 1 170 228 190 247 1.54e-12 69.3
MsaG003830 AT5G44190.2 54.237 59 26 1 173 231 145 202 1.71e-12 68.6
MsaG003830 AT1G49560.1 55.556 63 28 0 175 237 192 254 2.40e-12 67.8
MsaG003830 AT2G40970.1 57.407 54 22 1 175 228 105 157 2.63e-12 66.6
MsaG003830 AT1G25550.2 59.259 54 22 0 172 225 206 259 2.64e-12 67.0
MsaG003830 AT5G05090.1 55.556 54 23 1 175 228 81 133 3.54e-12 66.6
MsaG003830 AT3G62670.1 53.968 63 25 1 175 233 211 273 4.57e-12 67.8
MsaG003830 AT5G59570.2 50.820 61 29 1 168 228 134 193 4.89e-12 66.6
MsaG003830 AT5G59570.1 50.820 61 29 1 168 228 134 193 4.89e-12 66.6
MsaG003830 AT3G10760.1 55.556 54 23 1 175 228 105 157 4.97e-12 67.0
MsaG003830 AT3G25790.1 54.545 55 25 0 180 234 201 255 6.12e-12 67.0
MsaG003830 AT3G46640.3 45.833 72 38 1 157 228 126 196 7.05e-12 66.2
MsaG003830 AT3G46640.2 45.833 72 38 1 157 228 126 196 7.54e-12 66.2
MsaG003830 AT3G46640.1 45.833 72 38 1 157 228 126 196 7.54e-12 66.2
MsaG003830 AT2G20570.1 55.932 59 25 1 173 231 151 208 1.18e-11 66.2
MsaG003830 AT4G37180.3 50.000 62 31 0 164 225 199 260 1.32e-11 65.1
MsaG003830 AT4G18020.8 50.769 65 31 1 167 231 288 351 1.84e-11 65.9
MsaG003830 AT4G18020.11 50.769 65 31 1 167 231 288 351 1.84e-11 65.9
MsaG003830 AT4G18020.4 50.769 65 31 1 167 231 288 351 1.84e-11 65.9
MsaG003830 AT4G18020.5 50.769 65 31 1 167 231 288 351 1.84e-11 65.9
MsaG003830 AT4G18020.10 50.769 65 31 1 167 231 288 351 1.84e-11 65.9
MsaG003830 AT4G18020.7 50.769 65 31 1 167 231 288 351 1.84e-11 65.9
MsaG003830 AT4G18020.3 50.769 65 31 1 167 231 288 351 1.95e-11 65.9
MsaG003830 AT4G18020.2 50.769 65 31 1 167 231 288 351 1.95e-11 65.9
MsaG003830 AT4G18020.1 50.769 65 31 1 167 231 288 351 1.95e-11 65.9
MsaG003830 AT4G18020.6 50.769 65 31 1 167 231 288 351 1.95e-11 65.9
MsaG003830 AT4G18020.9 50.769 65 31 1 167 231 288 351 1.95e-11 65.9
MsaG003830 AT2G20570.2 58.182 55 22 1 177 231 171 224 2.35e-11 65.5
MsaG003830 AT1G49190.1 52.632 57 24 1 175 228 215 271 2.93e-11 65.1
MsaG003830 AT1G13300.1 47.619 63 33 0 175 237 181 243 6.91e-11 63.5
MsaG003830 AT1G67710.3 57.692 52 22 0 177 228 121 172 8.90e-11 63.5
MsaG003830 AT1G67710.2 57.692 52 22 0 177 228 130 181 9.83e-11 63.5

Find 93 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCTCTGTGCTTGTATTC+TGG 0.101134 1:+62581545 None:intergenic
TGTATTCCACATGGTCATTC+TGG 0.149403 1:+62583835 None:intergenic
AGGAAGATCATTAACTTCTT+TGG 0.221222 1:+62583159 None:intergenic
CACATACCTTCTGATGATTC+TGG 0.228637 1:+62582757 None:intergenic
TTGCTCTGTGCTTGTATTCT+GGG 0.237413 1:+62581546 None:intergenic
CCGAGAAGTTCATTCCAATT+TGG 0.239899 1:+62583720 None:intergenic
ATGATTCTATTCAGCCAAAT+TGG 0.254038 1:-62583734 MsaT003830.1:CDS
CTCCTTGAAATCCGTTGTTT+TGG 0.268176 1:+62583785 None:intergenic
GTTGTTCATGAAGCCGCTTC+TGG 0.275179 1:+62581918 None:intergenic
TCTGGAGTCCAACGCATTCT+AGG 0.277206 1:+62583103 None:intergenic
GTATTCTGGGAACTTCCTTC+TGG 0.323449 1:+62581559 None:intergenic
GAATACAAGCACAGAGCAAA+AGG 0.372831 1:-62581542 MsaT003830.1:CDS
TTCTGGGTGGGAACATTATC+AGG 0.378603 1:+62583852 None:intergenic
ATTGAAGTCAACTTCTTCTC+TGG 0.390129 1:+62582307 None:intergenic
GTAGTCGACACAGAAGAATT+AGG 0.399816 1:+62583139 None:intergenic
GTATTCCACATGGTCATTCT+GGG 0.400152 1:+62583836 None:intergenic
AGAACTTCATGAGGCCTTTG+TGG 0.428094 1:-62583083 MsaT003830.1:CDS
AGCTACCGGTGAACTTGAGC+TGG 0.429993 1:-62581506 MsaT003830.1:CDS
TGCCAAAACAACGGATTTCA+AGG 0.441739 1:-62583787 MsaT003830.1:CDS
CCAAATTGGAATGAACTTCT+CGG 0.447252 1:-62583720 MsaT003830.1:CDS
ACAAAGGCCTCATGAAGTTC+TGG 0.447758 1:+62583085 None:intergenic
TATTCCCTCTTCCTCGATGC+AGG 0.454247 1:-62584048 MsaT003830.1:CDS
ATCAAGTTCAAGACACCCTT+AGG 0.470004 1:+62582939 None:intergenic
ATATGTAGAGGCTACTCCTA+AGG 0.470935 1:-62582955 MsaT003830.1:intron
TATACTGCTGCTGTGAAACC+TGG 0.475770 1:+62583197 None:intergenic
GATAGTGCCACACTTGATAG+TGG 0.493745 1:+62581661 None:intergenic
ACATAACGTCACTTTCTTGA+GGG 0.494013 1:+62583913 None:intergenic
TGATGATTCTGGTTTGTACC+TGG 0.500432 1:+62582768 None:intergenic
AGAAAGTGACGTTATGTCCT+GGG 0.500596 1:-62583907 MsaT003830.1:CDS
AATTGAAGAATATCTTCAAA+TGG 0.505497 1:+62583876 None:intergenic
ATTGTGGTTGTATAGGCCTA+AGG 0.506694 1:-62581975 MsaT003830.1:intron
TGCAGCCTGCATCGAGGAAG+AGG 0.509129 1:+62584043 None:intergenic
AGAAGCATTGCGGCTGCAGA+TGG 0.512926 1:-62581945 MsaT003830.1:CDS
TACAATGGTTCTGAAGTCTT+GGG 0.514053 1:-62583816 MsaT003830.1:CDS
AAGAAAGTGACGTTATGTCC+TGG 0.515386 1:-62583908 MsaT003830.1:CDS
TCTCTTGATGCAAGAGACCC+AGG 0.517615 1:-62583215 MsaT003830.1:CDS
AATTCCAACATCAGAAGTGT+TGG 0.526621 1:-62584014 MsaT003830.1:CDS
ATACAATGGTTCTGAAGTCT+TGG 0.526812 1:-62583817 MsaT003830.1:CDS
TTGAACTTGATGAAAGTTGA+GGG 0.529752 1:-62582927 MsaT003830.1:CDS
TTGTGGGTGGTGCGGTAAGT+TGG 0.530504 1:+62581476 None:intergenic
CGTTATCAGGCTGTTCAGAA+GGG 0.533325 1:+62583976 None:intergenic
TTCTCCCAGCTCAAGTTCAC+CGG 0.534132 1:+62581501 None:intergenic
GTCTCTTGCATCAAGAGAGA+AGG 0.539948 1:+62583220 None:intergenic
AATTTAGAAAACACAAATGA+AGG 0.541617 1:-62581625 MsaT003830.1:CDS
TCATCTGTAGATGCTTCCCT+TGG 0.544034 1:+62581722 None:intergenic
TCATGTGAAAAGCCATCTGC+AGG 0.546156 1:-62582892 MsaT003830.1:intron
ACGTTATCAGGCTGTTCAGA+AGG 0.551148 1:+62583975 None:intergenic
TTGTGGTTGTATAGGCCTAA+GGG 0.553041 1:-62581974 MsaT003830.1:intron
TCTCTTGCATCAAGAGAGAA+GGG 0.556360 1:+62583221 None:intergenic
AGATCATTAACTTCTTTGGA+TGG 0.562377 1:+62583163 None:intergenic
TTCAAATGGATCAGTTCCCC+AGG 0.563936 1:+62583890 None:intergenic
AATGCTTCTGTAATCCCCTT+AGG 0.564243 1:+62581959 None:intergenic
AATACAAGCACAGAGCAAAA+GGG 0.565422 1:-62581541 MsaT003830.1:CDS
TATGTAGAGGCTACTCCTAA+GGG 0.566576 1:-62582954 MsaT003830.1:intron
CTGATTGACTGCTTCCACAA+AGG 0.570201 1:+62583069 None:intergenic
GACATAACGTCACTTTCTTG+AGG 0.571867 1:+62583912 None:intergenic
TTCAGAAATATAGAACTGCC+AGG 0.576431 1:-62582786 MsaT003830.1:intron
GGAGATGATGCTAAAGCTAC+CGG 0.580790 1:-62581520 MsaT003830.1:CDS
AATCTAAGCCTAGAATGCGT+TGG 0.584166 1:-62583111 MsaT003830.1:CDS
AGATTCAGATTGAGAACCAA+GGG 0.585597 1:-62581738 MsaT003830.1:CDS
GTGGAAGCAGTCAATCAGCT+TGG 0.585863 1:-62583064 MsaT003830.1:CDS
AACAACGGATTTCAAGGAGT+GGG 0.590584 1:-62583781 MsaT003830.1:CDS
GAAGCAGTCAATCAGCTTGG+TGG 0.592276 1:-62583061 MsaT003830.1:CDS
AATGGTTCTGAAGTCTTGGG+TGG 0.595066 1:-62583813 MsaT003830.1:CDS
ACACTTGATAGTGGAGCAGA+AGG 0.602869 1:+62581670 None:intergenic
TATGACAGAACCAAAATCAC+AGG 0.603724 1:-62583688 MsaT003830.1:intron
CTTGAACTTGATGAAAGTTG+AGG 0.606827 1:-62582928 MsaT003830.1:CDS
GTGTCCAACACTTCTGATGT+TGG 0.607725 1:+62584010 None:intergenic
ATACTGCTGCTGTGAAACCT+GGG 0.609600 1:+62583198 None:intergenic
GCGGCTTCATGAACAACTTG+AGG 0.611704 1:-62581912 MsaT003830.1:intron
CAGATTCAGATTGAGAACCA+AGG 0.612607 1:-62581739 MsaT003830.1:CDS
AGAATGTATTTCTGAAGCAG+AGG 0.614653 1:+62583944 None:intergenic
TTCCACATGGTCATTCTGGG+TGG 0.615350 1:+62583839 None:intergenic
GCTACCGGTGAACTTGAGCT+GGG 0.615664 1:-62581505 MsaT003830.1:CDS
TCCACATGGTCATTCTGGGT+GGG 0.616235 1:+62583840 None:intergenic
GCAGCCTGCATCGAGGAAGA+GGG 0.625876 1:+62584044 None:intergenic
AAACAACGGATTTCAAGGAG+TGG 0.628215 1:-62583782 MsaT003830.1:CDS
GTTATCAGGCTGTTCAGAAG+GGG 0.629071 1:+62583977 None:intergenic
CAGAACCATTGTATTCCACA+TGG 0.632141 1:+62583826 None:intergenic
TGCAGATGGAACTCCAGAAG+CGG 0.633918 1:-62581931 MsaT003830.1:CDS
TACAAACCAGAATCATCAGA+AGG 0.635144 1:-62582763 MsaT003830.1:intron
TGTGGTTGTATAGGCCTAAG+GGG 0.641482 1:-62581973 MsaT003830.1:intron
TTCTGCTCCACTATCAAGTG+TGG 0.642875 1:-62581668 MsaT003830.1:CDS
ACTTCAGACAACATGCCAGA+AGG 0.644538 1:-62581574 MsaT003830.1:CDS
AATGACCATGTGGAATACAA+TGG 0.649000 1:-62583831 MsaT003830.1:CDS
TTGGACTCCAGAACTTCATG+AGG 0.657310 1:-62583092 MsaT003830.1:CDS
GAAAGTGACGTTATGTCCTG+GGG 0.673471 1:-62583906 MsaT003830.1:CDS
GGGTGGAAATGCCAAAACAA+CGG 0.682227 1:-62583796 MsaT003830.1:CDS
GTTTGAGCAACAGATGAAGT+CGG 0.685798 1:-62581698 MsaT003830.1:CDS
CTCTTGCATCAAGAGAGAAG+GGG 0.697249 1:+62583222 None:intergenic
AGGGGATTACAGAAGCATTG+CGG 0.709657 1:-62581955 MsaT003830.1:CDS
AATTGATGCAGCCTGCATCG+AGG 0.729819 1:+62584037 None:intergenic
TCCCACCCAGAATGACCATG+TGG 0.730540 1:-62583841 MsaT003830.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 62581453 62584081 62581453 ID=MsaG003830
Chr1 mRNA 62581453 62584081 62581453 ID=MsaT003830.1;Parent=MsaG003830
Chr1 exon 62581453 62581776 62581453 ID=MsaT003830.1.exon7;Parent=MsaT003830.1
Chr1 CDS 62581453 62581776 62581453 ID=cds.MsaT003830.1;Parent=MsaT003830.1
Chr1 exon 62581913 62581982 62581913 ID=MsaT003830.1.exon6;Parent=MsaT003830.1
Chr1 CDS 62581913 62581982 62581913 ID=cds.MsaT003830.1;Parent=MsaT003830.1
Chr1 exon 62582277 62582334 62582277 ID=MsaT003830.1.exon5;Parent=MsaT003830.1
Chr1 CDS 62582277 62582334 62582277 ID=cds.MsaT003830.1;Parent=MsaT003830.1
Chr1 exon 62582764 62582803 62582764 ID=MsaT003830.1.exon4;Parent=MsaT003830.1
Chr1 CDS 62582764 62582803 62582764 ID=cds.MsaT003830.1;Parent=MsaT003830.1
Chr1 exon 62582893 62582969 62582893 ID=MsaT003830.1.exon3;Parent=MsaT003830.1
Chr1 CDS 62582893 62582969 62582893 ID=cds.MsaT003830.1;Parent=MsaT003830.1
Chr1 exon 62583053 62583257 62583053 ID=MsaT003830.1.exon2;Parent=MsaT003830.1
Chr1 CDS 62583053 62583257 62583053 ID=cds.MsaT003830.1;Parent=MsaT003830.1
Chr1 exon 62583689 62584081 62583689 ID=MsaT003830.1.exon1;Parent=MsaT003830.1
Chr1 CDS 62583689 62584081 62583689 ID=cds.MsaT003830.1;Parent=MsaT003830.1
Gene Sequence

>MsaT003830.1

ATGTCTTCGTCTATTCCCTCTTCCTCGATGCAGGCTGCATCAATTAATTCCAACATCAGAAGTGTTGGACACATGTTTTCAACCCCTTCTGAACAGCCTGATAACGTCCATTTTTCCTCTGCTTCAGAAATACATTCTATGACATTCCCTCAAGAAAGTGACGTTATGTCCTGGGGAACTGATCCATTTGAAGATATTCTTCAATTTCCTGATAATGTTCCCACCCAGAATGACCATGTGGAATACAATGGTTCTGAAGTCTTGGGTGGAAATGCCAAAACAACGGATTTCAAGGAGTGGGTTGATCAGTTGATGTCAGTCGATGATGATTCTATTCAGCCAAATTGGAATGAACTTCTCGGTGATAACAATATGACAGAACCAAAATCACAGGATGCACAAATGTCCCCTTCTCTCTTGATGCAAGAGACCCAGGTTTCACAGCAGCAGTATATACTATCACTACCATCCAAAGAAGTTAATGATCTTCCTAATTCTTCTGTGTCGACTACATCGCAATCTAAGCCTAGAATGCGTTGGACTCCAGAACTTCATGAGGCCTTTGTGGAAGCAGTCAATCAGCTTGGTGGTAGTGAGAAGGCTACTCCTAAGGGTGTCTTGAACTTGATGAAAGTTGAGGGCCTTACCATCTATCATGTGAAAAGCCATCTGCAGAAATATAGAACTGCCAGGTACAAACCAGAATCATCAGAAGGAATTCCAGAGAAGAAGTTGACTTCAATCGACGAAATGCCGTCTATAGACTTAAAAACGCCTAAGGGGATTACAGAAGCATTGCGGCTGCAGATGGAACTCCAGAAGCGGCTTCATGAACAACTTGAGATTCAAAGAAATTTACAGATTCAGATTGAGAACCAAGGGAAGCATCTACAGATGATGTTTGAGCAACAGATGAAGTCGGATGAGCCTTCTGCTCCACTATCAAGTGTGGCACTATCTTCTCCAGTTGAAAATTTAGAAAACACAAATGAAGGTCACGAAAAAATTGGAATCAATGGCAGCACTTCAGACAACATGCCAGAAGGAAGTTCCCAGAATACAAGCACAGAGCAAAAGGGAGATGATGCTAAAGCTACCGGTGAACTTGAGCTGGGAGAAGACCAACTTACCGCACCACCCACAAAACGAGTGAAGACTGACAAGTAG
Protein sequence

>MsaT003830.1

MSSSIPSSSMQAASINSNIRSVGHMFSTPSEQPDNVHFSSASEIHSMTFPQESDVMSWGTDPFEDILQFPDNVPTQNDHVEYNGSEVLGGNAKTTDFKEWVDQLMSVDDDSIQPNWNELLGDNNMTEPKSQDAQMSPSLLMQETQVSQQQYILSLPSKEVNDLPNSSVSTTSQSKPRMRWTPELHEAFVEAVNQLGGSEKATPKGVLNLMKVEGLTIYHVKSHLQKYRTARYKPESSEGIPEKKLTSIDEMPSIDLKTPKGITEALRLQMELQKRLHEQLEIQRNLQIQIENQGKHLQMMFEQQMKSDEPSAPLSSVALSSPVENLENTNEGHEKIGINGSTSDNMPEGSSQNTSTEQKGDDAKATGELELGEDQLTAPPTKRVKTDK*